Academic literature on the topic 'Logan Express'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Logan Express.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Logan Express"
1
Barta, Jim, and Linda L'Ai. "Galleons, Magic Potions, and Quidditch: The Mathematics of Harry Potter." Teaching Children Mathematics 11, no. 4 (November 2004): 210–16. http://dx.doi.org/10.5951/tcm.11.4.0210.
Full textAbstract:
“All aboard the Hogwarts Express!” shout the students busily immersed in their studies. No, this is not a casting call for young actors and actresses living out their fantasies of starring in a new Harry Potter movie. Rather, the children are studying mathematics at the Edith Bowen Laboratory School in Logan, Utah. These fourth-grade students are developing mathematical activities around the topic of the child magician Harry Potter.
2
Velikiy, Yu, K. Netudyhata, and T. Pigar. "EXPRESS DIAGNOSTICS OF BANK LOAN PORTFOLIO QUALITY." Agrosvit, no. 21 (November 23, 2019): 22. http://dx.doi.org/10.32702/2306-6792.2019.21.22.
Full text
3
Dhaigude, Amol S., Soham Ray, and Dhrubojit Konwar. "ZeNXL express logistics: route optimization." Emerald Emerging Markets Case Studies 9, no. 2 (November 4, 2019): 1–20. http://dx.doi.org/10.1108/eemcs-05-2019-0134.
Full textAbstract:
Learning outcomes This case has four major learning outcomes using hands-on spreadsheet tool. First is to introduce and apply the Clarke and Wright’s Savings algorithm. Second is to conduct a cost-benefit analysis in transportation set up. Third is to find out the optimal route to be taken to fulfill given demand while satisfying time and capacity constraints. Finally, one has to optimize the number of vehicles required for daily operations. Case overview/synopsis Dhruvam, the protagonist of the case, working at ZeNXL, a third-party logistics service provider, was assigned the task of reducing the operational cost of the company as part of the new service offering called “Route optimization.” This new offering would help optimize vehicle delivery routes to meet daily customer demand. The launch of the new service offering was due in the next 10 days with client LG Electronics to be the first beneficiary. Complexity academic level MBA-1 (Logistics Management), MBA-2 (Route Optimization). This case provides an opportunity for instructors to introduce vehicle routing and scheduling as part of logistics management. Students are expected to use the data given in the case and exhibits to develop the optimal routes (using Clarke and Wright’s Savings algorithm) and conduct cost-benefit analysis. This case also provides insights on the challenges associated with start-up operations. Supplementary materials Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes. Subject code CSS 9: Operations and Logistics
4
Gómez, E., A. Rodríguez, C. De Frutos, J. N. Caamaño, N. Facal, and C. Díez. "178 IN VITRO CULTURE OF BOVINE EMBRYOS WITH NEUROTROPHINS." Reproduction, Fertility and Development 19, no. 1 (2007): 205. http://dx.doi.org/10.1071/rdv19n1ab178.
Full textAbstract:
Neurotrophins (NTs) mediate human embryonic stem (hES) cell survival and may also improve methods for hES cell derivation (Pyle et al. 2006 Nature Biotech. 24, 344–350) and quality of the inner cell mass (ICM). We searched published microarray data sets for tyrosine kinase receptors (TRK) (geo data base: GSM27469, GSM27470, GSM27471). The analysis suggested that bovine embryos in vitro at unspecified stages express TRKA, for nerve growth factor (NGF); TRKC, for neurotrophin-3 (NT3); and TRKB, for both neurotrophin-4 (NT4) and brain-derived neurotrophic factor (BDNF). NTs functionally cooperate among them and also with basic fibroblast growth factor (bFGF) (Pyle et al. 2006; Logan et al. 2006 Brain 129, 490–502). Experiments in progress include detection of TRK expression by RT-PCR at defined development stages, and analysis of embryonic development with NTs and without bFGF. In this work we cultured embryos matured and fertilized in vitro from slaughterhouse oocytes for 8 days in SOF medium with 6 g L-1 BSA and 2 ng mL-1 bFGF (negative control). Development was monitored and cells were differentially counted in the ICM and trophectoderm (TE) of expanded and hatched blastocysts. NTs were used during the whole culture at 20 ng mL-1 as single (4 experimental groups: NGF, NT3, NT4, and BDNF) or as pooled (1 group) NT compounds. Data (5 replicates; 1403 oocytes) were processed by GLM and Duncan's test, and expressed as LSM � SE (a,b: P < 0.05). At Day 3, no differences were found at the 5- to 8-cell stage, but NT3 and NT4 increased the proportions of embryos at the 8- to 16-cell stage (19.1 � 2.2 and 20.5 � 2.2, respectively, vs. 12.9 � 2.2 to 13.7 � 2.2 within the other groups). On Day 6, NT4 yielded more morulae than controls, BDNF, and NGF (35.3 � 2.7 vs. 26.1 � 2.7, 27.4 � 2.7, and 27.8 � 2.7, respectively), and did not differ from the other groups. NT4 produced more total Day 7 blastocysts than NT3 and BDNF (12.5 � 2.2 vs. 8.1 � 2.2 and 9.9 � 2.2, respectively), whereas there were no differences within medium and expanded blastocysts and Day 8 blastocysts. Proportions of morulae that formed blastocysts were appreciably lower than in concomitant experiments without bFGF. Pooled NTs showed decreased values as compared to some single NTs within the ICM [13.0 � 4.0 vs. 29.1 � 4.6 (NT3) and 24.9 � 4.3 (NGF)], the TE [89.0 � 8.4 vs. 120 � 11.9 (BDNF)], total cells [102.0 � 8.5 vs. 134.0 � 9.9 (NT3), and 140.0 � 12.1 (BDNF)], and tended to differ (P = 0.08) within ICM/total cells [13.1 � 3.1 vs. 21.6 � 3.6 (controls) and 22.2 � 3.6 (NT3)]. Controls differed from BDNF (TE: 88.1 � 9.8 vs. 120.2 � 11.9; total cells: 110.8 � 10.0 vs. 140.0 � 12.1, respectively), and from NT4 for ICM/total cells (21.6 � 3.6 vs. 11.5 � 2.9, respectively). NT4 is likely to exert a role during early embryonic development. However, these blastocysts showed decreased cell counts in the ICM, probably reflected in the pooled NTs group. Targeting proliferation stimuli specifically to the ICM is difficult to get when the ICM is enclosed in the embryo, in contrast with the isolated ICM or the derived stem cells. This work was supported by Grant AGL2005-04479.
5
Moore, G. W. Kent, Keith Alverson, and Gerald Holdsworth. "Variability in the climate of the Pacific Ocean and North America as expressed in the Mount Logan ice core." Annals of Glaciology 35 (2002): 423–29. http://dx.doi.org/10.3189/172756402781817185.
Full textAbstract:
AbstractIn this paper, we explore the climate signal contained in the annual snow-accumulation time series from a high-altitude ice core drilled on Mount Logan in the Saint Elias mountain range of western Canada. With the global meteorological fields from the U.S. National Centers for Environmental Prediction re-analysis, we construct composites of the atmospheric circulation and temperature patterns associated with anomalous snow accumulation at the Mount Logan site over the period 1948–87. These results confirm, with an independent method, previous work that identified the existence of a coherent upper-tropospheric circulation anomaly extending over much of the North Pacific Ocean and North America that is associated with snow accumulation at the site. This anomaly has a similar structure to that associated with the extratropical response to the El Niño–Southern Oscillation. Coherent structures consistent with this circulation pattern also exist in both air- and land-temperature fields. In particular, heavy (light) snow accumulation at the site is associated with warmer (colder) air and surface temperatures over the North Pacific Ocean and North America. Over the North Pacific, the sea-surface temperature anomaly associated with heavy snow accumulation at the site has a “horseshoe” pattern that is similar to that associated with the Pacific Decadal Oscillation.
6
Boatman, Angela, Brent J. Evans, and Adela Soliz. "Understanding Loan Aversion in Education." AERA Open 3, no. 1 (January 2017): 233285841668364. http://dx.doi.org/10.1177/2332858416683649.
Full textAbstract:
Although prior research has suggested that some students may be averse to taking out loans to finance their college education, there is little empirical evidence showing the extent to which loan aversion exists or how it affects different populations of students. This study provides the first large-scale quantitative evidence of levels of loan aversion in the United States. Using survey data collected on more than 6,000 individuals, we examine the frequency of loan aversion in three distinct populations. Depending on the measure, between 20 and 40% of high school seniors exhibit loan aversion with lower rates among community college students and adults not in college. Women are less likely to express loan-averse attitudes than men, and Hispanic respondents are more likely to be loan averse than White respondents.
7
Smith, Fred W. "GIL Express in Georgia: An Interlibrary Loan/Circulation Hybrid for Circulating Books." Journal of Interlibrary Loan,Document Delivery & Electronic Reserve 22, no. 2 (April 2012): 73–81. http://dx.doi.org/10.1080/1072303x.2012.690838.
Full text
8
Krajíček, Jan. "Lower bounds to the size of constant-depth propositional proofs." Journal of Symbolic Logic 59, no. 1 (March 1994): 73–86. http://dx.doi.org/10.2307/2275250.
Full textAbstract:
AbstractLK is a natural modification of Gentzen sequent calculus for propositional logic with connectives ¬ and ∧,∨ (both of bounded arity). Then for every d ≥ 0 and n ≥ 2, there is a set of depth d sequents of total size O(n3+d) which are refutable in LK by depth d + 1 proof of size exp(O(log2n)) but such that every depth d refutation must have the size at least exp(nΩ(1)). The sets express a weaker form of the pigeonhole principle.
9
Fysal, M. Ahamed, P. Sahaya Jenitha, and S. Ganapathy Subramanian. "Online MCA (Pok)." International Journal on Cybernetics & Informatics 10, no. 2 (May 31, 2021): 81–90. http://dx.doi.org/10.5121/ijci.2021.100210.
Full textAbstract:
The purpose of the project only for students knowledge. Students using the username & password to login the website .These project have some new features. In this blog, Daily morning uploading top 5 technological news and entrepreneurs motivational videos, Technical videos, Technological Q&A, Alumni projects with them the contacts, user can get marks and daily timetables.The newly joined students knows about MCA Rank holder, cultural, symposium and students can express their opinions and can say theirneeds.The students can knows about the In-campus drives and alumni placements. This document is meant for describing all the features and procedures that were followed while developing the system. This document specially mentions the details of the project how it was developed, the primary requirement, as well as various features and functionalities of the project and the procedures followed in achieving these objectives. The purpose of the project only for student knowledge. When students comes to website. They will learn one thing about technology. Its prominent intensity of this project .Students using the username & password to login the website .These project have some new features. In this blog, Daily morning uploading 5 technological news and entrepreneurs motivational videos, technical videos, technologic Q&A, Alumni project and download the project with zip file. The newly joined students knows about MCA cultural, symposium and students can express their opinions and can say their needs. The student can knows about the alumni placements details and contact with their social media.
10
Zaida Gonzalez Abrante. "La segunda persona objetivadora (tú) en las redes sociales y revistas: datos de textos escritos." Borealis – An International Journal of Hispanic Linguistics 9, no. 2 (November 10, 2020): 209–31. http://dx.doi.org/10.7557/1.9.2.5599.
Full textAbstract:
La segunda persona del singular tú en el español puede adquirir dos tipos de referencias diferentes, dependiendo de las situaciones discursivas y comunicativas en las que se utilice. El primer uso es el deíctico, es decir, aquella que apela directamente al oyente. El segundo es el uso no deíctico, es decir, el que se utiliza no para referirse a un oyente real, sino para transmitir significados que tradicionalmente se han denominado como generales o inespecíficos. Este fenómeno ha sido denominado como tú genérico o inespecífico. Sin embargo, en investigaciones recientes que han profundizado en las propiedades cognitivas y discursivas de este uso, se ha optado por denominarlo como tú objetivador debido a que se trata de un recurso comunicativo que tiende a expresar el discurso de forma objetiva mediante la prominencia cognitiva. Se analizará el uso de la segunda persona del singular tú como recurso objetivador en el discurso escrito de las redes sociales y publicaciones periódicas, partiendo de la idea de que, al ser la expresión de opiniones, argumentaciones o ideas propias y la propia experiencia personal las funciones que más aparecen en las redes sociales y revistas, este debe usarse para construir un significado que ayude a logar tal objetivo comunicativo. Este presenta dos variantes: expresa y omitida, lo que implica que cada una de estas expresa un significado diferente. Además de esto, hay elementos gramaticales o inductores de genericidad que promueven ese uso objetivador del pronombre tú.
Dissertations / Theses on the topic "Logan Express"
1
Ara?jo, Sinara Carla da Silva. "Express?o heter?loga de biossurfactantes identificados em bibliotecas metagen?micas." Universidade Federal do Rio Grande do Norte, 2014. http://repositorio.ufrn.br/handle/123456789/19512.
Full textAbstract:
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2015-12-15T17:45:12Z
No. of bitstreams: 1
SinaraCarlaDaSilvaAraujo_DISSERT.pdf: 1885448 bytes, checksum: b4a8226a4f1778e4f626da3394657f9a (MD5)Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2015-12-29T20:51:37Z (GMT) No. of bitstreams: 1 SinaraCarlaDaSilvaAraujo_DISSERT.pdf: 1885448 bytes, checksum: b4a8226a4f1778e4f626da3394657f9a (MD5)
Made available in DSpace on 2015-12-29T20:51:37Z (GMT). No. of bitstreams: 1 SinaraCarlaDaSilvaAraujo_DISSERT.pdf: 1885448 bytes, checksum: b4a8226a4f1778e4f626da3394657f9a (MD5) Previous issue date: 2014-08-22
Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq
Os microrganismos apresentam uma imensa diversidade gen?tica e est?o presentes em toda biosfera, no entanto cerca de 1% das esp?cies pode ser cultivada por t?cnicas laboratoriais padr?o. A metagen?mica tornou poss?vel o acesso direto ao genoma microbiano derivado de amostras ambientais, utilizando t?cnicas independentes de cultivo. A metodologia permite obter informa??o funcional de prote?nas, assim como a identifica??o de potenciais produtos com interesse biotecnol?gico e de novos recursos biol?gicos industrialmente explor?veis, a exemplo novas solu??es para impactos ambientais. ?reas contaminadas com petr?leo s?o caracterizadas por um grande ac?mulo de hidrocarbonetos e surfactantes s?o utilizados para sua biorremedia??o. Sendo assim, a abordagem metagen?nima foi utilizada para selecionar genes envolvidos no processo de degrada??o e biossurfacta??o de hidrocarbonetos. Em um trabalho anterior, o DNA ambiental (eDNA) foi extra?do de amostras de solo coletadas em duas diferentes ?reas (Caatinga e rio salino) do Rio Grande do Norte (Brasil), as bibliotecas metagen?micas foram constru?das e analisadas funcionalmente. Os clones capazes de degradar o ?leo foram avaliados quanto ? capacidade de sintetizar biossurfactante. O clone foi sequenciado e an?lise da sequencia revelou uma ORF com 897 pb, 298 amino?cidos e prote?na de peso molecular pr?ximo a 34 kDa. A busca por homologia no GenBank revelou similaridade com a sequ?ncia que codifica uma prote?na hipot?tica de representantes da fam?lia Halobacteriaceae, que foram mostradas recentemente como produtoras de biossurfactantes. A presen?a da sequ?ncia codificante inserida e do fen?tipo adquirido foram confirmadas. Primers foram desenhados e suas ORFs amplificadas por PCR. Em seguida, foram subclonadas em vetor de express?o pETDuet-1, contendo uma cauda de histidina, para express?o e posterior purifica??o da prote?na de interesse. Os testes foram realizados para confirma??o da atividade de biossurfactante e degrada??o de hidrocarbonetos e apresentaram resultados positivos. O ensaio de imunodetec??o (western blot) com a utiliza??o do anticorpo monoclonal Anti-His? confirmou a presen?a da prote?na ambiental. Esse estudo foi o primeiro a relatar uma poss?vel prote?na com atividade biossurfactante obtida a partir de uma abordagem metagen?mica.
The microorganisms have a vast genetic diversity and they are present throughout the biosphere, however, only about 1% of the species can be cultivated by traditional cultivation techniques. Within this diversity there is a huge pool genetic and biological being explored. The metagenomics has enabled direct access to microbial genome derived from environmental samples using independent methods of cultivation. The methodology enables to obtain functional information about the proteins, as well as identify potential products with biotechnological interest and new industrially exploitable biological resources, such as new solutions to environmental impacts. Oil-contaminated areas are characterized by a large accumulation of hydrocarbons and surfactants may be used for bioremediation. Thus, the metagenomic approach was used in this study in order to select genes involved in the degradation and hydrocarbon emulsification. In a previous work, the environmental DNA (eDNA) was extracted from soil samples collected from two different areas (Caatinga and Saline River) of Rio Grande do Norte (Brazil), the metagenomic libraries were constructed and functionally analyzed. The clone able to degrade the oil was evaluated for the ability to synthesize biosurfactants. The sequence analysis revealed an ORF with 897 bp, 298 amino acids and a protein with around 34 kDa. The search for homology in GenBank revealed sequence similarity with a hypothetical protein of representatives Halobacteriaceae family, who were recently shown as strains producing biosurfactants. The presence of the inserted coding sequence and the acquired phenotype was confirmed. Primers were designed and the ORF amplified by PCR. The ORF was subcloned into pETDuet-1 expression vector for subsequent purification of the protein of interest containing a histidine tail. The tests performed to confirm the biosurfactant activity and the ability of hydrocarbon degradation showed positive results. The immunodetection test (western blot) using the monoclonal AntiHis? confirmed the presence of the environmental protein. This study was the first to report a possible protein with biosurfactant activity obtained from a metagenomic approach
2
Cobacho, Nicole Berwanger. "Produ????o heter??loga dos pept??deos antimicrobianos Cm-p5 e Cn-AMP1 em sistema procarioto." Universidade Cat??lica de Bras??lia, 2017. https://bdtd.ucb.br:8443/jspui/handle/tede/2294.
Full textAbstract:
Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-11-09T10:26:59Z
No. of bitstreams: 1
NicoleBerwangerCobachoDissertacao2017.pdf: 2681393 bytes, checksum: 06c28d06b89b63056ccfd84351dd8d36 (MD5)Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-11-09T11:17:54Z (GMT) No. of bitstreams: 1 NicoleBerwangerCobachoDissertacao2017.pdf: 2681393 bytes, checksum: 06c28d06b89b63056ccfd84351dd8d36 (MD5)
Made available in DSpace on 2017-11-09T11:17:55Z (GMT). No. of bitstreams: 1 NicoleBerwangerCobachoDissertacao2017.pdf: 2681393 bytes, checksum: 06c28d06b89b63056ccfd84351dd8d36 (MD5) Previous issue date: 2017-08-22
UCB
The resistance of microorganisms to commonly used antibiotics has increased dramatically in recent years, suggesting that we will soon enter a post-antibiotic era where no therapy currently used will be effective in the fighting against infection. Thus, the search and study of new drugs and models of action of compounds that prevent or reduce the development of pathogens is essential. In this context, antimicrobial peptides (PAMs) appear as a new generation of compounds that demonstrate a great therapeutic potential. These molecules present themselves in low concentration in the organism of origin, making their isolation from natural sources an expensive and often impracticable process. In order to obtain a greater quantity of these peptides, recombinant expression via the heterologous system can be considered an efficient alternative in terms of time, cost and productivity. In 2009, Mandal et al. isolated three coconut water (Coco nucifera) peptides, called Cn-AMP1-2 and -3. Among them, Cn-AMP1 presented better activity against bacteria and fungi of medical importance. Subsequently, Lopez-Abarrategui et al. in 2012 isolated peptides with antimicrobial activity from the mollusk Cenchritis muricatus called Cm-p1 and Cm-p2. From these, a series of variant peptides were theoretically proposed in silico and evaluated against Candida albicans and the peptide named Cm-p5 demonstrated, among them, better antifungal activity. In this work, several constructs containing the peptides Cm-p5 and Cn-AMP1 were drawn in tandem and inserted into the pETSUMO (Life Technologies) vector. Genes were expressed in large quantities in strains derived from Escherichia coli BL21 (DE3) and after affinity column purification, they were evaluated, still fused with SUMO protein, against pathogenic microorganisms. However, none of the expressed peptides demonstrated bactericidal activity against the strains evaluated.
A resist??ncia dos microrganismos aos antibi??ticos que s??o comumente utilizados vem aumentando drasticamente nos ??ltimos anos, sugerindo que brevemente, entraremos em uma era p??s-antibi??ticos onde terapias utilizadas atualmente n??o ser??o mais eficientes no combate a infec????es. Desta forma, a procura e o estudo de novas drogas e modelos de a????o de compostos que impe??am ou reduzam o desenvolvimento de pat??genos ?? essencial. Neste contexto, os pept??deos antimicrobianos (PAMs) surgem como uma nova gera????o de compostos que demonstram um grande potencial terap??utico. Essas mol??culas apresentam-se em baixa concentra????o no organismo de origem, tornando o seu isolamento a partir de fontes naturais um processo dispendioso e, por muitas vezes, invi??vel. Para a obten????o de maior quantidade desses pept??deos, a express??o recombinante via sistema heter??logo pode ser considerada uma alternativa eficiente em rela????o ao tempo, custo e produtividade. Em 2009, Mandal e colaboradores isolaram tr??s pept??deos da agua de coco (Coco nucifera), denominados Cn-AMP1 -2 e -3. Dentre eles, o Cn-AMP1 apresentou melhor atividade contra bact??rias e fungos de import??ncia medica. Posteriormente, Lopez-Abarrategui e colaboradores em 2012 isolaram pept??deos com atividade antimicrobiana do molusco Centrichis muricatus denominados Cm-p1 e Cm-p2. A partir destes, uma serie de pept??deos variantes foram teoricamente propostos in silico e avaliados contra C. albicans sendo que o pept??deo nominado o Cm-p5 demonstrou, entre todos, melhor atividade antif??ngica. Neste trabalho, v??rias constru????es contendo os pept??deos Cm-p5 e Cn-AMP1 foram desenhadas em tandem e inseridas no vetor pETSUMO (Life Technologies). Os genes foram expressos em grandes quantidades em cepas derivadas de Escherichia coli BL21(DE3) e ap??s purifica????o em coluna de afinidade, os mesmos foram avaliados, ainda fusionados com a prote??na SUMO, contra microrganismos patog??nicos. No entanto, nenhum dos pept??deos expressos demonstrou atividade bactericida contra as cepas avaliadas.
3
TOMAZETT, Patrícia Kott. "An€álise da resposta de Paracoccidioides brasiliensis a diferentes tipos de agentes estressores e osmoreguladores e express o heteró‚loga e localizaçムo de β-1.3-glicana sintase." Universidade Federal de Goiás, 2010. http://repositorio.bc.ufg.br/tede/handle/tde/1501.
Full textAbstract:
Made available in DSpace on 2014-07-29T15:25:12Z (GMT). No. of bitstreams: 1
patricia kott.pdf: 7658177 bytes, checksum: ad9ad7831fb8596c7e55d3b1f0eee117 (MD5)
Previous issue date: 2010-02-26Paracoccidioides brasiliensis is a thermo-dimorphic human pathogenic fungus that lives at 23”C in the mycelium phase (infecting phase) and at 37”C in the yeast phase (parasite phase). In attempt to survive, the cell wall of fungi can change its composition and/or structure in response to environmental stress by compensatory mechanisms. The molecules involved in these mechanisms are possible target for the development of effective antifungal agents. In P. brasiliensis, the main components of the cell wall are glucans and chitin polymers. These polymers make a primary barrier that is responsible for the structural integrity and form of the cell wall. In this work the behavior of P. brasiliensis was evaluated against stress conditions with the aim of study, for the first time, the mechanisms used by this fungus in the maintenance of the cell wall integrity. Our results shown that P. brasiliensis yeast cells are sensitive to cell wall stressors calcofluor white (CFW), congo red (CR), SDS, KCl, NaCl and sorbitol. There was an increase in the PbFKS1 transcripts expression and in the content of cell wall β- 1,3-glicana after treatment with all stressor agents. After treatment with SDS and KCl the PbGFA1 transcripts expression and the cell wall GlcNAc residues also increased. The transcript expression of PbGEL3 was also evaluated being increased after treatment with CFW, NaCl and sorbitol. Thus we showed that these molecules are involved in the maintenance of the cell wall against stress conditions. Apart from these analyses we obtained the active recombinant protein PbFks1pc. Through the anti-PbFks1pc antibody we performed immunocitolocalization assays. These experiments revealed the localization of PbFks1p in regions of apical growth in the mycelium phase and in the cell surface in yeast phase.
Paracoccidioides brasiliensis Š um fungo patog‰nico e termodim‚rfico que se apresenta na forma de micŠlio a 23”C (forma infectante) e na forma de levedura a 37”C (forma parasit€ria). A parede celular de fungos Š capaz de alterar sua composiƒ o e atŠ mesmo sua estrutura em resposta a condiƒ•es ambientais de estresse atravŠs de mecanismos compensat‚rios. As prote…nas envolvidas nestes mecanismos s o poss…veis alvos para o desenvolvimento de agentes antifˆngicos espec…ficos. Em P. brasiliensis, os principais pol…meros que constituem a parede celular s o glicanas e quitina. Estes pol…meros formam uma barreira prim€ria que Š respons€vel pela integridade estrutural e forma da parede celular. Neste trabalho, o comportamento de P. brasiliensis diante de situaƒ•es de estresse foi investigado com o intuito de estudar, pela primeira vez, os mecanismos utilizados por este fungo na manutenƒ o da integridade da parede celular. Nossos resultados mostram que cŠlulas leveduriformes de P. brasiliensis s o sens…veis aos agentes estressores da parede celular calcofluor white (CFW), congo red (CR), SDS, NaCl, KCl e sorbitol. Houve um aumento na express o de transcritos de PbFKS1 e no conteˆdo do pol…mero de β-1,3-glicana na parede celular ap‚s tratamento com todos os agentes estressores. Ap‚s tratamento com SDS e KCl a express o de transcritos de PbGFA1 assim como o conteˆdo de res…duos de GlcNAc na parede celular tambŠm aumentaram. A express o dos transcritos de PbGEL3 tambŠm foi avaliada estando aumentada ap‚s tratamento com CFW, NaCl e sorbitol. Desta forma, mostramos que estas molŠculas est o envolvidas na manutenƒ o da parede celular diante de situaƒ•es de estresse. AlŠm destas an€lises, a prote…na recombinante PbFks1pc ativa foi obtida. AtravŠs do anticorpo anti-PbFks1pc realizamos ensaios de imunocitolocalizaƒ o. Estes experimentos revelaram a presenƒa de PbFks1p em regi•es de crescimento apical no micŠlio e na superf…cie celular em cŠlulas leveduriformes.
4
Hsu, I.-Jou, and 許怡柔. "Construction of Byblis to express secretory proteins." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5111021%22.&searchmode=basic.
Full textAbstract:
碩士國立中興大學
生物科技學研究所
107
Carnivorous plants usually grow in a nutrient-poor environment. They have evolved various traps to capture and digest other organisms for nutritional purpose. Byblis guehoi, also known as rainbow grass, can grow fast and is easy to culture. Moreover, the secretory glandular trichomes that distributed all over the leaf can secrete viscous dewdrops which contain abundant enzymes including protease, phosphatase, galactosidase, esterase/lipase etc. Owing to the powerful secretion characteristics of Byblis, we aim to construct it as a bioreactor to produce secretory proteins. By RNA-seq analysis and de novo sequence assembly, we obtain 123,944 sequence contigs. We look for genes having high RPKM (Reads Per Kilobase per Million mapped reads) and encoding proteins with typical signal sequences at their N-terminus. Moreover, to confirm that which genes are for the secretome, the surface dewdrops were collected and the proteins were identified by mass spectrometry (LC/MS/MS). By cross comparison of the RNA-seq and LC/MS/MS data, nine genes were chosen as the targets for promoter hunting. qPCR experiment revealed the genome size of Byblis to be about 8.8 Gb, 23 times that of the rice genome. By nanopore sequencing, 3.1 GB genomic sequences were obtained and the promoter regions for the first three candidate genes were found for primer design. The promoter fragments, each with 2~3 kb, were PCR amplified, subcloned and sequencing confirmed. Together with the signal peptide, the vector systems will be constructed for future insertion of foreign genes. On the other hand, in order to generate transgenic Byblis, we established its Agrobacterium-mediated transformation protocol using GUS-intron vector as a reporter. About 30% callus did form green callus 2 weeks after transformation. Some shoot regenerated to normal looking transgenic Byblis, with GUS-staining confirmed. However, the shoot formation process was extremely long that took several months to go with unstable frequency. Therefore direct regeneration from tissue together with vacuum-aided Agrobacterium-mediated transformation are underway, hope to further improve the transformation efficiency of Byblis.
Books on the topic "Logan Express"
5
ValuSource. Loan Express (Valusource Accounting Software Products S.). John Wiley & Sons Inc, 1995.
Find full text
6
ValuSource. Loan Express (Valusource Accounting Software Products S.). John Wiley & Sons Inc, 1995.
Find full text
8
Loan Express Continuing Professional Education Course: 1995 DOS. Wiley Valusource, 1995.
Find full text
10
Loan Express Release 1.0 (Valusource Accounting Software Products S.). John Wiley & Sons, 1994.
Find full textBook chapters on the topic "Logan Express"
1
Pournelle, Jerry. "Dealing with Spam." In 1001 Computer Words You Need to Know. Oxford University Press, 2004. http://dx.doi.org/10.1093/oso/9780195167757.003.0012.
Full textAbstract:
If you’ve used e-mail for any time at all, you’ve no doubt had your inbox deluged with messages pitching aphrodisiacs, mortgages, junk stocks, pornography, and substances that claim to help you lose weight in your sleep. But don’t despair. Such messages don’t have to be part of the Internet experience. You can avoid them, or certainly reduce the annoyance level, with a few simple measures. Some spammers harvest e-mail addresses from the Internet by using a “spider” (also known as a robot or crawler)—a computer program that creeps through the World Wide Web collecting information you’d prefer to keep private. So the better you are at hiding your address from faceless prowlers, the less spam you will receive. 1. If your Internet Service Provider gives you the choice, create a long, fairly complicated address preceding the @; don’t just use your first name: donquixote@example.com is better than don@example.com. Better still is donquixotedelamancha@example. com. It may be unwieldy, but your friends can always use a nickname—or just click on your address. Happily, longer addresses confuse spammers, who—without having exact user accounts in hand—use a “dictionary attack” to find them, testing the validity of every possible address from lists of common words and names: arline@example.com, barbara@example.com, and so forth. 2. Again, unless some online service makes it mandatory, don’t use your e-mail address as part of a login name or identity. However convenient that might make it when logging in to eBay, it’s equally convenient for spammers. 3. Use a second, public address for nonpersonal mail and for public posting. Create a free account with Hotmail, Yahoo, or Excite, or set up another screen name if you use either AOL or another Internet Service Provider that allows you to have multiple addresses. This keeps your main address private and exposes only your public address to spammers. Check the public account at least occasionally, in case something innocent has come in. Then massively delete the rest. It’s important to learn how your e-mail client (the program you use to send and receive e-mail) handles thorough deleting; in Outlook Express, for example, you have to delete an e-mail you don’t want from your inbox, delete it from the “Deleted” folder, and then compact folders before you are genuinely rid of it.
2
"ners increased (about 50 in 1770 and 204 in 1830). Unlike most firms of that period, it was not a family business. The Turning Period (1791-1820) The accounting system used in the 18th century achieved two main tasks: it computed the wealth (inventory) and enrichment (receipts and payments) of the partners, and it kept the internal movements of goods and cash under control with a comprehen sive system of vouchers. However, there does not seem to be any reckoning of costs before 1820. The Company waited for over 150 years before calcu lating a cost amount for its products. If one wants to prove the importance of that turning point, the quotes below from two man agers are evidence. In 1793, i.e. during the French Revolution, the Company delivered to each associate an “Instruction to help the interested parties in the Manufacture of glass with the declaration form they had to fill in about their interest in that trade, according to the Compulsory Loan Act of the 24th of August." Such a docu ment4 had four aims, the most important of which was providing knowledge of the profit of the year 1793. According to the order-in-council, “the benefit was that which went beyond the interests of the funds invested.” The interest was easily known (5% of the net worth) thanks to the inventory. But as regards the evaluation of benefits, the calculation seemed quite impossible from the au thors’ instruction: Things do not go with glass as they do with cloth, for which the cost is known even before we put it on the frame. Glass, on the contrary, never preserves its original value. The flaws entail scraps, that is why the benefit of the glass production is a random result and it is impos sible to calculate it. In 1829, the Baron Roederer, a director of the Company of Saint-Quirin, expressed quite an opposite point of view when he described the problems raised by the possible merger of the two competing companies.5 It seems that in this case, everything could be reduced on both sides to the calculation of a square foot of glass." In Accounting in France (RLE Accounting), 253. Routledge, 2014. http://dx.doi.org/10.4324/9781315871042-21.
Full textConference papers on the topic "Logan Express"
1
Topaloğlu, Mustafa. "An Evaluation of Turkish Mortgage System from the Perspective of Global Economic Crisis." In International Conference on Eurasian Economies. Eurasian Economists Association, 2011. http://dx.doi.org/10.36880/c02.00359.
Full textAbstract:
Turkish mortgage system was established by the law number 5582 and the title of "The Law Amending the Laws Related to Housing Finance System" in 2007. Even though the entry into force of this act expressed as "Pay the rent as the landlord-performing”, no bring up short of the interest rates of a housing loan were observed. In fact, Mortgage application could not be branch out yet. The distinguishing feature of the mortgage system, mortgage collateral pools of consumer loans with guaranteed by mortgage backed securities to be issued, sold in the capital market, also called the mortgage money is the safeguard of cheap funds. Using this fund for financing provided by banks as a result of re-housing resource for the consumer to pay the cost of housing loan interest rate is relatively go into a decline.
Meanwhile, after the abundance of finance in the world, the so-called subprime mortgage, loans to non-qualified borrower, triggered the world economic crisis occurred. May well be, Turkey was unimpressed the crisis because of the not being set secondary mortgage market. All the public in charge of economy has introduced prevention of packages of measures.