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Journal articles on the topic 'Long alpha helix'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Funato, Noriko, Yuki Taga, Lindsay E. Laurie, Chisa Tometsuka, Masashi Kusubata, and Kiyoko Ogawa-Goto. "The Transcription Factor HAND1 Is Involved in Cortical Bone Mass through the Regulation of Collagen Expression." International Journal of Molecular Sciences 21, no. 22 (2020): 8638. http://dx.doi.org/10.3390/ijms21228638.

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Temporal and/or spatial alteration of collagen family gene expression results in bone defects. However, how collagen expression controls bone size remains largely unknown. The basic helix-loop-helix transcription factor HAND1 is expressed in developing long bones and is involved in their morphogenesis. To understand the functional role of HAND1 and collagen in the postnatal development of long bones, we overexpressed Hand1 in the osteochondroprogenitors of model mice and found that the bone volumes of cortical bones decreased in Hand1Tg/+;Twist2-Cre mice. Continuous Hand1 expression downregula

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Ruggiero, F., J. Comte, C. Cabanas, and R. Garrone. "Structural requirements for alpha 1 beta 1 and alpha 2 beta 1 integrin mediated cell adhesion to collagen V." Journal of Cell Science 109, no. 7 (1996): 1865–74. http://dx.doi.org/10.1242/jcs.109.7.1865.

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A large variety of cells adhere to and spread on specific regions within the triple helix of collagens, mainly via alpha 1 beta 1 and alpha 2 beta 1 integrins. Disruption of collagen triple helical integrity generally affects the efficiency of cell adhesion on different collagens including collagen V. This report addresses the question of the importance of the linear sequence of the constitutive alpha-chains versus the triple helical conformation in the recognition of collagen V binding sites. To investigate this question, in vitro renaturation of the isolated alpha 1 (V) and alpha 2 (V) chain

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3

DEWEY, T. GREGORY. "SEQUENCE GENERATING FUNCTIONS AND THE MULTIFRACTAL FORMALISM: APPLICATIONS TO BIOPOLYMERS." Fractals 03, no. 01 (1995): 9–22. http://dx.doi.org/10.1142/s0218348x95000035.

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Helix-coil configurations in biopolymers can be represented as a symbolic sequence of helix and coil units. A given configuration can be generated by progressing down the branches of a binary tree. The probability weighting of the branches in the tree will depend on the specific model under consideration. The multifractal nature of this binary tree can be described by an adaptation of a statistical mechanical formalism that uses sequence generating functions. It is seen that the sequence generating function approach is equivalent to transfer matrix methods for processes involving one-step memo

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4

Klein, G., CA Muller, E. Tillet, ML Chu, and R. Timpl. "Collagen type VI in the human bone marrow microenvironment: a strong cytoadhesive component." Blood 86, no. 5 (1995): 1740–48. http://dx.doi.org/10.1182/blood.v86.5.1740.bloodjournal8651740.

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Collagen type VI, which forms characteristic microfibrillar structures, is assembled from three individual alpha(VI) chains that form a short triple helix and two adjacent globular domains. Expression of all three alpha (VI) collagen chains in the human bone marrow (BM) microenvironment could be detected by chain-specific antibodies in tissue sections and in the adherent stromal layer of long-term BM cultures. In functional studies, collagen type VI was shown to be a strong adhesive substrate for various hematopoietic cell lines and light-density BM mononuclear cells. The adhesive site within

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5

Muljaningsih, Sri, Multifiah Multifiah, and Ajeng Kartika Galuh. "Synergicity of Triple Helix Creative Economy Model in Malang." MIMBAR : Jurnal Sosial dan Pembangunan 34, no. 1 (2018): 185–94. http://dx.doi.org/10.29313/mimbar.v34i1.3327.

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The Triple Helix concept, the government-academics-business relationship, has been established in response to the 2005-2025 Creative Economy Long-term National Development Plan, especially in the mission of economic value creation of creative innovation. This study explores the factors influencing sinergicity of the triple helix model with SMEs' organic product in Malang as its object. This research was conducted with mixed methodology of FGD and survey. Validity and reliability test were performed based on the Cronbach's Alpha value and after being fulfilled, continue with analysis factor. Th

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Cieślik-Boczula, Katarzyna. "Alpha-helix to beta-sheet transition in long-chain poly- l -lysine: Formation of alpha-helical fibrils by poly- l -lysine." Biochimie 137 (June 2017): 106–14. http://dx.doi.org/10.1016/j.biochi.2017.03.006.

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7

Yanaka-Okada, Saeko, Emiko Sano, Norio Naruse, et al. "Targeted-glycosylation of "non-core region" of interleukin-11 modulates its biological function. (57.15)." Journal of Immunology 186, no. 1_Supplement (2011): 57.15. http://dx.doi.org/10.4049/jimmunol.186.supp.57.15.

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Abstract Human interleukin-11 (hIL-11) is a pleiotropic cytokine administered to patients for recovering low platelet counts. From a structural point of view hIL-11 belongs to the long-helix cytokine superfamily, which is characterized by a conserved core motif consisting of four alpha-helices. We have investigated the region of hIL-11 that does not belong to the alpha-helical bundle motif, which we have termed “non-core region”. The interleukin was N- or O-glycosylated at specific positions within the non-core region, and the functional consequences of these modifications were examined in cel

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Weinzierl, Robert O. J. "The Bridge Helix of RNA Polymerase Acts as a Central Nanomechanical Switchboard for Coordinating Catalysis and Substrate Movement." Archaea 2011 (2011): 1–7. http://dx.doi.org/10.1155/2011/608385.

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The availability ofin vitroassembly systems to produce recombinant archaeal RNA polymerases (RNAPs) offers one of the most powerful experimental tools for investigating the still relatively poorly understood molecular mechanisms underlying RNAP function. Over the last few years, we pioneered new robot-based high-throughput mutagenesis approaches to study structure/function relationships within various domains surrounding the catalytic center. The Bridge Helix domain, which appears in numerous X-ray structures as a 35-amino-acid-long alpha helix, coordinates the concerted movement of several ot

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9

Keller, Paul W., Marc C. Johnson, and Volker M. Vogt. "Mutations in the Spacer Peptide and Adjoining Sequences in Rous Sarcoma Virus Gag Lead to Tubular Budding." Journal of Virology 82, no. 14 (2008): 6788–97. http://dx.doi.org/10.1128/jvi.00213-08.

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ABSTRACT All orthoretroviruses encode a single structural protein, Gag, which is necessary and sufficient for the assembly and budding of enveloped virus-like particles from the cell. The Gag proteins of Rous sarcoma virus (RSV) and human immunodeficiency virus type 1 (HIV-1) contain a short spacer peptide (SP or SP1, respectively) separating the capsid (CA) and nucleocapsid (NC) domains. SP or SP1 and the residues immediately upstream are known to be critical for proper assembly. Using mutagenesis and electron microscopy analysis of insect cells or chicken cells overexpressing RSV Gag, we def

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10

Batebi, Hossein, Guillermo Pérez-Hernández, Sabrina N. Rahman, et al. "Mechanistic insights into G-protein coupling with an agonist-bound G-protein-coupled receptor." Nature Structural & Molecular Biology 31, no. 11 (2024): 1692–701. https://doi.org/10.1038/s41594-024-01334-2.

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G-protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by promoting guanine nucleotide exchange. Here, we investigate the coupling of G proteins with GPCRs and describe the events that ultimately lead to the ejection of GDP from its binding pocket in the Gα subunit, the rate-limiting step during G-protein activation. Using molecular dynamics simulations, we investigate the temporal progression of structural rearrangements of GDP-bound G<sub>s</sub> protein (G<sub>s</sub>·GDP; hereafter G<sub>s</sub><sup>GDP</sup>) upon coupling to the β<sub>2</sub>-adrene

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11

Kazaks, Andris, I.-Na Lu, Sophie Farinelle, et al. "Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates." BMC Biotechnology 17, no. 1 (2017): 79. https://doi.org/10.1186/s12896-017-0396-8.

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<strong>Background: </strong>The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus strains. The long alpha helix (LAH) is an attractive vaccine component since it is one of the most conserved influenza hemagglutinin (HA) stalk regions. For an improved immune response, the LAH domain from H3N2 strain has been incorporated into virus-like particles (VLPs) derived from hepatitis B virus core protein (HBc) using recently developed tandem core te

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Castañeda-Arriaga, Romina, Adrián Domínguez-Castro, JinGyu Lee, J. Raul Alvarez-Idaboy, and Nelaine Mora-Diez. "Chemical repair of protein carbon-centred radicals: long-distance dynamic factors." Canadian Journal of Chemistry 94, no. 12 (2016): 1119–26. http://dx.doi.org/10.1139/cjc-2016-0230.

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The thermodynamic and kinetic study of the repair reactions of three damaged aliphatic amino acids (alanine, valine, and leucine) with dihydrolipoic acid (DHLA) in a polar and a nonpolar solvent is presented in this work. Two simplified protein models were explored in the most common conformations (alpha helix and beta sheet). Calculations are performed at the M06-2X-SMD/6-31++G(d,p) level of theory. DHLA has shown to be an excellent antioxidant repair agent through hydrogen-transfer reaction involving the thiol groups, with rate constants close to diffusion control in most cases. The stabilit

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13

Elliott, S., T. Lorenzini, D. Chang, et al. "Fine-structure epitope mapping of antierythropoietin monoclonal antibodies reveals a model of recombinant human erythropoietin structure." Blood 87, no. 7 (1996): 2702–13. http://dx.doi.org/10.1182/blood.v87.7.2702.bloodjournal8772702.

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We have isolated and mapped the rHuEPO epitopes for three noncompeting anti-EPO monoclonal antibodies (MoAbs). The MoAb 9G8A recognizes a linear epitope that includes amino acids 13, 16, and 17. MoAb F12 recognizes a conformational epitope that includes amino acids 31 through 33, 86 through 91, and 138. MoAb D11 recognizes a conformational epitope that includes amino acids 64 through 78 and 99 through 110. MoAb D11 neutralizes rHuEPO activity which suggests that its epitope may contain the receptor binding domain. Analysis of the effect of mutations on folding allowed the identification of bur

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14

SHALYGIN, SERGEI, KATHERINE J. KAVULIC, NICOLE PIETRASIAK, et al. "Neotypification of Pleurocapsa fuliginosa and epitypification of P. minor (Pleurocapsales): resolving a polyphyletic cyanobacterial genus." Phytotaxa 392, no. 4 (2019): 245. http://dx.doi.org/10.11646/phytotaxa.392.4.1.

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Strains with complete morphological match to Pleurocapsa fuliginosa and P. minor were isolated from Oahu, with another strain matching P. minor isolated from a wet rock face in Utah. Phylogenetically these baeocyte and pseudofilament producing strains fell in a single well-supported clade among a number of pleurocapsalean strains. They were sister to a clade of baeocyte-producing strains that lack the ability to form psuedofilaments and likely belong in an as-yet-to-be-described genus. Strains putatively named Pleurocapsa are scattered throughout the Pleurocapsales and Chroococcales, indicatin

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15

Noegel, A. A., S. Rapp, F. Lottspeich, M. Schleicher, and M. Stewart. "The Dictyostelium gelation factor shares a putative actin binding site with alpha-actinins and dystrophin and also has a rod domain containing six 100-residue motifs that appear to have a cross-beta conformation." Journal of Cell Biology 109, no. 2 (1989): 607–18. http://dx.doi.org/10.1083/jcb.109.2.607.

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The 120-kD gelation factor and alpha-actinin are among the most abundant F-actin cross-linking proteins in Dictyostelium discoideum. Both molecules are homodimers and have extended rod-like configurations that are respectively approximately 35 and 40 nm long. Here we report the complete cDNA sequence of the 120-kD gelation factor which codes for a protein of 857 amino acids. Its calculated molecular mass is 92.2 kD which is considerably smaller than suggested by its mobility in SDS-PAGE. Analysis of the sequence shows a region that is highly homologous to D. discoideum alpha-actinin, chicken f

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16

Davatelis, G., P. Tekamp-Olson, S. D. Wolpe, et al. "Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties." Journal of Experimental Medicine 167, no. 6 (1988): 1939–44. http://dx.doi.org/10.1084/jem.167.6.1939.

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In the course of studies on cachectin/TNF being conducted in our laboratory, a novel macrophage product has been detected and characterized. Termed macrophage inflammatory protein or MIP, this protein appears to be an endogenous mediator of the inflammatory events induced by endotoxin. A cDNA cloned probe for this protein has been isolated from a lambda gt10 phage library prepared from poly(A)+ RNA obtained of endotoxin-induced RAW264.7 cells. The sequence codes for a 92 amino acid-long polypeptide, of which 69 amino acids correspond to the mature product. The sequence predicts a molecular wei

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17

DEWEY, T. GREGORY. "FRACTAL ASPECTS OF PROTEIN STRUCTURE AND DYNAMICS." Fractals 01, no. 02 (1993): 179–89. http://dx.doi.org/10.1142/s0218348x93000198.

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Proteins have well-defined three dimensional structures which are dictated by their amino acid sequence. Despite this great specificity, general structural and dynamic properties exist. Scaling relationships for the radius of gyration and surface area of a large data set of proteins are demonstrated in this work. These results show that proteins scale as collapsed polymers. Thermal fluctuations are examined for two different proteins by an analysis of the Debye-Waller factors derived from X-ray crystallographic data. Long-range correlations exist between fluctuations along the backbone. A diso

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18

Meyer, R. K., and U. Aebi. "Bundling of actin filaments by alpha-actinin depends on its molecular length." Journal of Cell Biology 110, no. 6 (1990): 2013–24. http://dx.doi.org/10.1083/jcb.110.6.2013.

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Cross-linking of actin filaments (F-actin) into bundles and networks was investigated with three different isoforms of the dumbbell-shaped alpha-actinin homodimer under identical reaction conditions. These were isolated from chicken gizzard smooth muscle, Acanthamoeba, and Dictyostelium, respectively. Examination in the electron microscope revealed that each isoform was able to cross-link F-actin into networks. In addition, F-actin bundles were obtained with chicken gizzard and Acanthamoeba alpha-actinin, but not Dictyostelium alpha-actinin under conditions where actin by itself polymerized in

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19

Sauer, U. H., D. P. San, and B. W. Matthews. "Tolerance of T4 lysozyme to proline substitutions within the long interdomain alpha-helix illustrates the adaptability of proteins to potentially destabilizing lesions." Journal of Biological Chemistry 267, no. 4 (1992): 2393–99. http://dx.doi.org/10.1016/s0021-9258(18)45892-9.

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20

Curzon, Arie Yehuda, Andrey Shirak, Ayana Benet-Perlberg, et al. "Absence of Figla-like Gene Is Concordant with Femaleness in Cichlids Harboring the LG1 Sex-Determination System." International Journal of Molecular Sciences 23, no. 14 (2022): 7636. http://dx.doi.org/10.3390/ijms23147636.

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Oreochromis niloticus has been used as a reference genome for studies of tilapia sex determination (SD) revealing segregating genetic loci on linkage groups (LGs) 1, 3, and 23. The master key regulator genes (MKR) underlying the SD regions on LGs 3 and 23 have been already found. To identify the MKR in fish that segregate for the LG1 XX/XY SD-system, we applied short variant discovery within the sequence reads of the genomic libraries of the Amherst hybrid stock, Coptodon zillii and Sarotherodon galilaeus, which were aligned to a 3-Mbp-region of the O. aureus genome. We obtained 66,372 variant

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Gallegos, M. T., R. Schleif, A. Bairoch, K. Hofmann, and J. L. Ramos. "Arac/XylS family of transcriptional regulators." Microbiology and Molecular Biology Reviews 61, no. 4 (1997): 393–410. http://dx.doi.org/10.1128/mmbr.61.4.393-410.1997.

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The ArC/XylS family of prokaryotic positive transcriptional regulators includes more than 100 proteins and polypeptides derived from open reading frames translated from DNA sequences. Members of this family are widely distributed and have been found in the gamma subgroup of the proteobacteria, low- and high-G + C-content gram-positive bacteria, and cyanobacteria. These proteins are defined by a profile that can be accessed from PROSITE PS01124. Members of the family are about 300 amino acids long and have three main regulatory functions in common: carbon metabolism, stress response, and pathog

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Maaloum, M., D. Chretien, E. Karsenti, and J. K. Horber. "Approaching microtubule structure with the scanning tunneling microscope (STM)." Journal of Cell Science 107, no. 11 (1994): 3127–31. http://dx.doi.org/10.1242/jcs.107.11.3127.

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We demonstrate that the scanning tunneling microscope can be used to obtain information about arrangement of tubulin subunits in the microtubule wall. Long rows of subunits with a periodicity of 3.8 +/- 0.4 nm were clearly visible in the images of microtubules. The separation between the rows of subunits was 4.8 +/- 0.4 nm. Close inspection of two images revealed another periodicity of 7.8 +/- 0.4 nm in the contour levels of the protofilaments. This indicates that alpha and beta tubulin monomers can be resolved. In these areas the monomers were arranged according to a ‘B-type’ lattice. Scannin

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Nakai, A., M. Satoh, K. Hirayoshi, and K. Nagata. "Involvement of the stress protein HSP47 in procollagen processing in the endoplasmic reticulum." Journal of Cell Biology 117, no. 4 (1992): 903–14. http://dx.doi.org/10.1083/jcb.117.4.903.

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The 47,000-D collagen-binding glycoprotein, heat shock protein 47 (HSP47), is a stress-inducible protein localized in the ER of collagen-secreting cells. The location and collagen-binding activity of this protein led to speculation that HSP47 might participate in collagen processing. Chemical crosslinking studies were used to test this hypothesis both before and after the perturbation of procollagen processing. The association of procollagen with HSP47 was demonstrated using cleavable bifunctional crosslinking reagents. HSP47 and procollagen were shown to be coprecipitated by the treatment of

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Hu, Jianrao, Mingfu Cao, and Jiong Chen. "Characterization of the cDNA encoding a BPI/LBP homologue in venom gland of the hundred-pace snake Deinagkistrodon acutus." Current Zoology 55, no. 5 (2009): 376–82. http://dx.doi.org/10.1093/czoolo/55.5.376.

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Abstract Bactericidal/permeability-increasing protein (BPI) and LPS-binding protein (LBP) play an important role in host defence. Current evidence shows that BPI/ LBP may be widely existed in different cells and tissue types of animals. A full-length cDNA clone encoding a BPI/LBP homologue (dBPI), 1757 bp in size, was characterized in venom gland of the hundred-pace snake Deinagkistrodon acutus. Its deduced amino acid sequence of 417 residues had 13.8% - 21.5% identity to BPI like 1 (BPILl) and BPI like 3 (BPIL3) of other animals. Conserved cysteine residues which are involved in disulfide bon

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Xiong, Yingxin, Zhirui Liu, Yuanqiang Wang, Jiawei Wang, Xing Zhou, and Xiaohui Li. "Development and Evaluation of a Water-Free In Situ Depot Gel Formulation for Long-Acting and Stable Delivery of Peptide Drug ACTY116." Pharmaceutics 16, no. 5 (2024): 620. http://dx.doi.org/10.3390/pharmaceutics16050620.

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In situ depot gel is a type of polymeric long-acting injectable (pLAI) drug delivery system; compared to microsphere technology, its preparation process is simpler and more conducive to industrialization. To ensure the chemical stability of peptide ACTY116, we avoided the use of harsh conditions such as high temperatures, high shear mixing, or homogenization; maintaining a water-free and oxygen-free environment was also critical to prevent hydrolysis and oxidation. Molecular dynamics (MDs) simulations were employed to assess the stability mechanism between ACTY116 and the pLAI system. The init

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Hauck, Nastasja, Josiane Kirpach, Christina Kiefer, et al. "Applying Unique Molecular Identifiers in Next Generation Sequencing Reveals a Constrained Viral Quasispecies Evolution under Cross-Reactive Antibody Pressure Targeting Long Alpha Helix of Hemagglutinin." Viruses 10, no. 4 (2018): 148. http://dx.doi.org/10.3390/v10040148.

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Yuan, H. S., S. S. Wang, W. Z. Yang, S. E. Finkel, and R. C. Johnson. "The structure of Fis mutant Pro61Ala illustrates that the kink within the long alpha-helix is not due to the presence of the proline residue." Journal of Biological Chemistry 269, no. 46 (1994): 28947–54. http://dx.doi.org/10.1016/s0021-9258(19)61998-8.

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Burnett, R. C., and D. E. Geraghty. "Structure and expression of a divergent canine class I gene." Journal of Immunology 155, no. 9 (1995): 4278–85. http://dx.doi.org/10.4049/jimmunol.155.9.4278.

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Abstract We have isolated and characterized a canine class I MHC (dog leukocyte Ag, DLA) gene, DLA-79. The deduced protein sequence shares only 65% identity with a previously published canine class I cDNA, designated DLA-A, and exhibits 64% amino acid identity with the HLA-A, -B, -C consensus. The peptide-binding region of DLA-79 is unusual. Three of four highly conserved tyrosine residues (Tyr7, 59, 159, and 171), proposed to interact with the N terminus of peptide-Ag, are substituted. Additionally, the long alpha-helix lining the peptide-binding region in the alpha 1 domain contains one more

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Bateman, J. F., D. Chan, I. Moeller, M. Hannagan та W. G. Cole. "A 5′ splice site mutation affecting the pre-mRNA splicing of two upstream exons in the collagen COL1A1 gene. Exon 8 skipping and altered definition of exon 7 generates truncated proα1(I) chains with a non-collagenous insertion destabilizing the triple helix". Biochemical Journal 302, № 3 (1994): 729–35. http://dx.doi.org/10.1042/bj3020729.

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A heterozygous de novo G to A point mutation in intron 8 at the +5 position of the splice donor site of the gene for the pro alpha 1(I) chain of type I procollagen, COL1A1, was defined in a patient with type IV osteogenesis imperfecta. The splice donor site mutation resulted not only in the skipping of the upstream exon 8 but also unexpectedly had the secondary effect of activating a cryptic splice site in the next upstream intron, intron 7, leading to re-definition of the 3′ limit of exon 7. These pre-mRNA splicing aberrations cause the deletion of exon 8 sequences from the mature mRNA and th

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Wyss, Stéphanie, Antony S. Dimitrov, Frédéric Baribaud, Terri G. Edwards, Robert Blumenthal, and James A. Hoxie. "Regulation of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Fusion by a Membrane-Interactive Domain in the gp41 Cytoplasmic Tail." Journal of Virology 79, no. 19 (2005): 12231–41. http://dx.doi.org/10.1128/jvi.79.19.12231-12241.2005.

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ABSTRACT Truncation of the human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) gp41 cytoplasmic tail (CT) can modulate the fusogenicity of the envelope glycoprotein (Env) on infected cells and virions. However, the CT domains involved and the underlying mechanism responsible for this “inside-out” regulation of Env function are unknown. HIV and SIV CTs are remarkably long and contain amphipathic alpha-helical domains (LLP1, LLP2, and LLP3) that likely interact with cellular membranes. Using a cell-cell fusion assay and a panel of HIV Envs with stop codons at various positi

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Pal, Aruna, Arjava Sharma, T. K. Bhattacharya, P. N. Chatterjee, and A. K. Chakravarty. "Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle." Molecular Biology International 2011 (October 25, 2011): 1–13. http://dx.doi.org/10.4061/2011/507346.

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CD14 is an important molecule for innate immunity that can act against a wide range of pathogens. The present paper has characterized CD14 gene of crossbred (CB) cattle (Bos indicus×Bos taurus). Cloning and sequence analysis of CD14 cDNA revealed 1119 nucleotide long open reading frame encoding 373 amino acids protein and 20 amino acids signal peptide. CB cattle CD14 gene exhibited a high percentage of nucleotide identity (59.3–98.1%) with the corresponding mammalian homologs. Cattle and buffalo appear to have diverged from a common ancestor in phylogenetic analysis. 25 SNPs with 17 amino acid

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Trapp-Fragnet, Laëtitia, Katia Courvoisier, Sylvie Rémy, G. Le Pape, Fabien Loustalot, and Caroline Denesvre. "Identification of Marek’s Disease Virus VP22 Tegument Protein Domains Essential for Virus Cell-to-Cell Spread, Nuclear Localization, Histone Association and Cell-Cycle Arrest." Viruses 11, no. 6 (2019): 537. http://dx.doi.org/10.3390/v11060537.

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VP22 is a major tegument protein of alphaherpesviruses encoded by the UL49 gene. Two properties of VP22 were discovered by studying Marek’s disease virus (MDV), the Mardivirus prototype; it has a major role in virus cell-to-cell spread and in cell cycle modulation. This 249 AA-long protein contains three regions including a conserved central domain. To decipher the functional VP22 domains and their relationships, we generated three series of recombinant MDV genomes harboring a modified UL49 gene and assessed their effect on virus spread. Mutated VP22 were also tested for their ability to arres

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Chen, Shaoheng, Dan Zheng, Changgui Li, et al. "Protection against Multiple Subtypes of Influenza Viruses by Virus-Like Particle Vaccines Based on a Hemagglutinin Conserved Epitope." BioMed Research International 2015 (2015): 1–12. http://dx.doi.org/10.1155/2015/901817.

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We selected the conserved sequence in the stalk region of influenza virus hemagglutinin (HA) trimmer, the long alpha helix (LAH), as the vaccine candidate sequence, and inserted it into the major immunodominant region (MIR) of hepatitis B virus core protein (HBc), and, by using theE. coliexpression system, we prepared a recombinant protein vaccine LAH-HBc in the form of virus-like particles (VLP). Intranasal immunization of mice with this LAH-HBc VLP plus cholera toxin B subunit with 0.2% of cholera toxin (CTB*) adjuvant could effectively elicit humoral and cellular immune responses and protec

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Lein, P. J., D. Higgins, D. C. Turner, L. A. Flier, and V. P. Terranova. "The NC1 domain of type IV collagen promotes axonal growth in sympathetic neurons through interaction with the alpha 1 beta 1 integrin." Journal of Cell Biology 113, no. 2 (1991): 417–28. http://dx.doi.org/10.1083/jcb.113.2.417.

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We have examined the effects of collagen IV on the morphological development of embryonic rat sympathetic neurons in vitro. In short-term (less than or equal to 24 h) culture, collagen IV accelerated process outgrowth, causing increases in the number of neurites and total neuritic length. Analysis of proteolytic fragments of collagen IV indicated that the NC1 domain was nearly as active as the intact molecule in stimulating process outgrowth; in contrast, the 7S domain and triple helix-rich fragments of collagen IV were inactive. Moreover, anti-NC1 antiserum inhibited neuritic outgrowth on col

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Mezhenskaya, Daria, Irina Isakova-Sivak, Tatiana Gupalova, et al. "A Live Probiotic Vaccine Prototype Based on Conserved Influenza a Virus Antigens Protect Mice against Lethal Influenza Virus Infection." Biomedicines 9, no. 11 (2021): 1515. http://dx.doi.org/10.3390/biomedicines9111515.

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Background: Due to the highly variable nature of the antigenic properties of the influenza virus, many efforts have been made to develop broadly reactive influenza vaccines. Various vaccine platforms have been explored to deliver conserved viral antigens to the target cells to induce cross-reactive immune responses. Here, we assessed the feasibility of using Enterococcus faecium L3 as a bacterial vector for oral immunization against influenza virus. Methods: we generated two vaccine prototypes by inserting full-length HA2 (L3-HA2) protein or its long alpha helix (LAH) domain in combination wit

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Kirsteina, Anna, Inara Akopjana, Janis Bogans, et al. "Construction and Immunogenicity of a Novel Multivalent Vaccine Prototype Based on Conserved Influenza Virus Antigens." Vaccines 8, no. 2 (2020): 197. http://dx.doi.org/10.3390/vaccines8020197.

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Influenza, an acute, highly contagious respiratory disease, remains a significant threat to public health. More effective vaccination strategies aimed at inducing broad cross-protection not only against seasonal influenza variants, but also zoonotic and emerging pandemic influenza strains are urgently needed. A number of conserved protein targets to elicit such cross-protective immunity have been under investigation, with long alpha-helix (LAH) from hemagglutinin stalk and ectodomain of matrix protein 2 ion channel (M2e) being the most studied ones. Recently, we have reported the three-dimensi

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Snetkov, Xenia, Tafhima Haider, Dejan Mesner, Nicholas Groves, Schuyler B. van Engelenburg, and Clare Jolly. "A Conserved Tryptophan in the Envelope Cytoplasmic Tail Regulates HIV-1 Assembly and Spread." Viruses 14, no. 1 (2022): 129. http://dx.doi.org/10.3390/v14010129.

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The HIV-1 envelope (Env) is an essential determinant of viral infectivity, tropism and spread between T cells. Lentiviral Env contain an unusually long 150 amino acid cytoplasmic tail (EnvCT), but the function of the EnvCT and many conserved domains within it remain largely uncharacterised. Here, we identified a highly conserved tryptophan motif at position 757 (W757) in the LLP-2 alpha helix of the EnvCT as a key determinant for HIV-1 replication and spread between T cells. Alanine substitution at this position potently inhibited HIV-1 cell–cell spread (the dominant mode of HIV-1 disseminatio

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Hannah, Brian P., Ekaterina E. Heldwein, Florent C. Bender, Gary H. Cohen, and Roselyn J. Eisenberg. "Mutational Evidence of Internal Fusion Loops in Herpes Simplex Virus Glycoprotein B." Journal of Virology 81, no. 9 (2007): 4858–65. http://dx.doi.org/10.1128/jvi.02755-06.

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ABSTRACT Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) is one of four glycoproteins necessary and sufficient for HSV cellular entry. Recently, the crystal structures of HSV-1 gB and vesicular stomatitis virus glycoprotein G were determined. Surprisingly, the two proteins share remarkable structural homology. Both proteins are homotrimeric and center about a long alpha-helix, features reminiscent of class I fusion proteins, such as influenza virus hemagglutinin or paramyxovirus F. However, these structures revealed that G has internal fusion loops, similar to the fusion loops of the c

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Cui, Can, Cancan Yan, Ailin Wang, et al. "Understanding the Inhibition Mechanism of Lignin Adsorption to Cellulase in Terms of Changes in Composition and Conformation of Free Enzymes." Sustainability 15, no. 7 (2023): 6057. http://dx.doi.org/10.3390/su15076057.

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The adsorption of lignin to cellulase is the major obstacle in the sugar-platform conversion of lignocellulosic bioresources. In this study, the adsorption behavior of untreated and pretreated lignin samples from corn stover to cellulase was investigated, in particular the effects of lignin adsorption on the composition and spatial conformation of free enzymes were explored. The results showed that pretreatments decreased the hydrophobic groups contents of lignin, i.e., aromatic ring, ether and carbonyl, as well as the content of ionizable group, i.e., carboxyl, which reduced its hydrophobicit

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Peterson, C. B., and H. K. Schachman. "Long range effects of amino acid substitutions in the catalytic chain of aspartate transcarbamoylase. Localized replacements in the carboxyl-terminal alpha-helix cause marked alterations in allosteric properties and intersubunit interactions." Journal of Biological Chemistry 267, no. 4 (1992): 2443–50. http://dx.doi.org/10.1016/s0021-9258(18)45899-1.

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Madrigal, J. A., M. P. Belich, R. J. Benjamin, et al. "Molecular definition of a polymorphic antigen (LA45) of free HLA-A and -B heavy chains found on the surfaces of activated B and T cells." Journal of Experimental Medicine 174, no. 5 (1991): 1085–95. http://dx.doi.org/10.1084/jem.174.5.1085.

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A monomoprhic monoclonal antibody (LA45 antibody) reactive with "a new activation-induced surface structure on human T lymphocytes" (LA45 antigen) that resembled free class I heavy chains has recently been described (Schnabl, E., H. Stockinger, O. Majdic, H. Gaugitsch, I.J.D. Lindley, D. Maurer, A. Hajek-Rosenmayr, and W. Knapp. 1990. J. Exp. Med. 171:1431). This antibody was used to clone a class I-like heavy chain (LA45 gene) from the HUT 102 tumor cell, which paradoxically did not give rise to the LA45 antigen on transfection into monkey COS cells. We show here that the LA45 gene is HLA-Aw6

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Shacklett, Barbara L., Claudia Jo Weber, Karen E. S. Shaw, et al. "The Intracytoplasmic Domain of the Env Transmembrane Protein Is a Locus for Attenuation of Simian Immunodeficiency Virus SIVmac in Rhesus Macaques." Journal of Virology 74, no. 13 (2000): 5836–44. http://dx.doi.org/10.1128/jvi.74.13.5836-5844.2000.

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ABSTRACT The human and simian immunodeficiency virus (HIV-1 and SIVmac) transmembrane proteins contain unusually long intracytoplasmic domains (ICD-TM). These domains are suggested to play a role in envelope fusogenicity, interaction with the viral matrix protein during assembly, viral infectivity, binding of intracellular calmodulin, disruption of membranes, and induction of apoptosis. Here we describe a novel mutant virus, SIVmac-M4, containing multiple mutations in the coding region for the ICD-TM of pathogenic molecular clone SIVmac239. Parental SIVmac239-Nef+ produces high-level persisten

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Zhu, Yanjin, Bangting Pan, Xixi Fei, et al. "The Biological Characteristics and Differential Expression Patterns of TSSK1B Gene in Yak and Its Infertile Hybrid Offspring." Animals 13, no. 2 (2023): 320. http://dx.doi.org/10.3390/ani13020320.

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This study aimed to investigate the spatially and temporally expressed patterns and biological characteristics of TSSK1B in male yaks and explore the potential correlation between TSSK1B and male sterility of the yak hybrid offspring (termed cattle–yak). First, the coding sequence (CDS) of TSSK1B was cloned by RT-PCR, and bioinformatics analysis was conducted with relevant software. Quantitative real-time PCR (RT-qPCR) was employed to detect the expression profile of TSSK1B in various tissues of male adult yaks, the spatiotemporal expression of TSSK1B in different stages of yak testes, and the

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Dib, Elie G., Nicholas Hill, Jessica Wollard, et al. "Using bioinformatic tools to examine similarities between human PDL-1 protein and common tobacco (Nicotiana tabacum)." Journal of Clinical Oncology 38, no. 5_suppl (2020): 70. http://dx.doi.org/10.1200/jco.2020.38.5_suppl.70.

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70 Background: Human CD274 receptor, known as programmed cell death 1 ligand 1 (RefSeq NP_054862.1), is an inhibitory ligand for Programmed cell death protein 1 (PD1). We aimed in this bioinformatic experiment to identify a homologous PDL-1 protein in plants. Methods: We chose to use tBLASTn because it compares a protein query sequence against a nucleotide sequence database dynamically translated in all six reading frames (both strands). We chose the EST database and restricted our search to plants (Viridiplantae). Results: Using the FASTA sequence of PDL-1 protein, the tBLASTn of the CD274 pr

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Yang, Ruochen, Chunhui Duan, Yunxia Guo, et al. "Sequence analysis and mRNA expression of prolactin receptor gene isoforms in different tissues of sheep during lactation and the post-weaning period." PeerJ 9 (July 29, 2021): e11868. http://dx.doi.org/10.7717/peerj.11868.

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Few studies on mRNA expression of the prolactin receptor (PRLR) isoforms in different tissues of sheep were reported. The objective of this study was to analyze the gene sequence and mRNA expression of PRLR isoforms in the uterus, mammary gland, ovary, spleen and lymph tissue of ewes during the lactation and post-weaning periods. Ten lactating crossbred ewes (Dorper×Hu sheep) with twin lambs were used in this study. Five ewes were chosen randomly and slaughtered at mid-lactation (35 days after lambing). The remaining five ewes were slaughtered on the 5th day after weaning. Samples of uterus, m

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Obiaga, Chinwe, Niren Patel, Hernan Sabio та ін. "Diagnostic Complications of Thalassemic Hb Showa-Yakushiji (β110[G12] Leu→Pro),Observed in An African American Child with Co-Inherited Hb B2 (δ16[A13]Gly→Arg) and α-Thal-2 (-α3.7 deletion): Effects of Triple Globin Gene Abnormality On Phenotype." Blood 114, № 22 (2009): 2558. http://dx.doi.org/10.1182/blood.v114.22.2558.2558.

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Abstract Abstract 2558 Poster Board II-535 Hemoglobinopathies can usually be classified under two major categories. Qualitative abnormalities resulting from missense mutations in the globin genes, leading to the production of mostly asymptomatic Hb variants, and quantitative defects, which result in the synthesis of structurally normal globin chains in reduced quantities (thalassemias). However there are known globin chain variants that cause alterations of the globin structure as well as a decrease in synthesis, leading to a thalassemic phenotype. The occurrence of multiple abnormalities of α

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Carvajal, Luis A., Daniela Ben-Neriah, Adrien Senecal, et al. "Dual Inhibition of Mdmx and Mdm2 Using an Alpha-Helical P53 Stapled Peptide (ALRN-6924) As a Novel Therapeutic Strategy in Acute Myeloid Leukemia." Blood 130, Suppl_1 (2017): 795. http://dx.doi.org/10.1182/blood.v130.suppl_1.795.795.

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Abstract While the TP53 tumor suppressor gene is mutated in more than 50% of human tumors, in Acute Myeloid Leukemia (AML) TP53 mutations are rare, occurring in less than 10% of cases. Yet, functional inactivation of wild-type p53 due to non-mutational abnormalities occurs frequently in AML and other hematological malignancies. A major mechanism of p53 inactivation results from the overexpression of its endogenous inhibitors MDMX (also known as MDM4, HDMX, and HDM4) and MDM2 which are frequently overexpressed in various p53 wild-type human cancers, including AML. Strikingly, MDMX has been repo

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Civettini, Ivan, Paola Corti, Arianna Zappaterra, et al. "Hemoglobin Monza: A New Variant of Unstable Hemoglobin." Blood 142, Supplement 1 (2023): 5273. http://dx.doi.org/10.1182/blood-2023-184846.

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Introduction: Unstable hemoglobins are variants with structural abnormalities; while not hindering protein formation, they lead to protein destabilization and can cause hemolytic crises. Most unstable hemoglobins are caused by point mutations that result in the premature dissociation of heme from the globin chain and usually involve His residues (Weatherall et al. 2001 Nat.Rev.Genetics; Scheps et al. 2018 EJH). Here a new unstable hemoglobin, named Hb-Monza is presented, exhibiting a long duplication in the HBB gene without apparent alterations in α-β subunit interaction or involvement of spec

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Quintero-Hernández, Verónica. "Editorial 6(21) AyTBUAP. La próxima pandemia: Bacterias multiresistentes a antibióticos." Alianzas y Tendencias BUAP 6, no. 21 (2021): i—vii. https://doi.org/10.5281/zenodo.5104911.

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<strong>RESUMEN</strong> Actualmente la humanidad se encuentra viviendo una pandemia inesperada provocada por el virus SARS-CoV-2 que causa la enfermedad denominada COVID-19. Afortunadamente se han desarrollado vacunas contra el SARS-CoV-2 en un tiempo récord, gracias al enorme esfuerzo de grandes laboratorios científicos y empresas farmacéuticas en todo el mundo. Sin embargo, aún se ve lejos su término y nos deja como lección que debemos vivir de ahora en adelante con la cultura de la prevención. Una vez que se supere esta pandemia, la humanida

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Attia, Abdelrahman M., Mohammad Saeid Rezaee-Zavareh, Soo Young Hwang, et al. "Novel Biomarkers for Early Detection of Hepatocellular Carcinoma." Diagnostics 14, no. 20 (2024): 2278. http://dx.doi.org/10.3390/diagnostics14202278.

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Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality globally. Most patients present with late diagnosis, leading to poor prognosis. This narrative review explores novel biomarkers for early HCC detection. We conducted a comprehensive literature review analyzing protein, circulating nucleic acid, metabolite, and quantitative proteomics-based biomarkers, evaluating the advantages and limitations of each approach. While established markers like alpha-fetoprotein (AFP), des-gamma-carboxy prothrombin, and AFP-L3 remain relevant, promising candidates include circulating tumor DNA,

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