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Journal articles on the topic 'Lowenstein–Jensen'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Almeida, Elisabete Aparecida de, Manoel Armando Azevedo dos Santos, Jorge Barros Afiune, Delurce Tadeu de Araújo Spada, and Fernando Augusto Fiuza de Melo. "Rendimento da cultura de escarro na comparação de um sistema de diagnóstico automatizado com o meio de Lowenstein-Jensen para o diagnóstico da tuberculose pulmonar." Jornal Brasileiro de Pneumologia 31, no. 3 (2005): 231–36. http://dx.doi.org/10.1590/s1806-37132005000300009.

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INTRODUÇÃO: A tuberculose permanece um problema de saúde pública mundial. OBJETIVO: Avaliar um sistema de diagnóstico automatizado de tuberculose, comparando-o com baciloscopia e cultura em meio de Lowenstein-Jensen. MÉTODO: Estudo comparativo entre os resultados obtidos no sistema automatizado, baciloscopia do escarro e em meio de Lowenstein-Jensen, em 844 amostras de escarro de setembro a dezembro de 1999, em centro de referência para tuberculose em São Paulo (SP). RESULTADOS: Das 844 amostras, 27,1% mostraram-se positivas para bacilo álcool-acidorresistente e 72,9% negativas. Nos cultivos e

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Tyas, Syanindhita Wikanthining, Linosefa Linosefa, Fika Tri Anggraini, and Andani Eka Putra. "Perbandingan Nilai Diagnostik Menggunakan Real Time Polymerase Chain Reaction dan Kultur Sebagai Baku Emas dalam Mendeteksi Mycobacterium tuberculosis." Jurnal Ilmu Kesehatan Indonesia 6, no. 2 (2025): 122–28. https://doi.org/10.25077/jikesi.v6i2.1400.

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Latar Belakang: Tuberkulosis merupakan penyakit menular yang disebabkan oleh Mycobacterium tuberculosis yang menduduki peringkat kedua penyebab kematian terbanyak. Faktor yang menyebabkan tingginya angka kejadian adalah keterlambatan dalam penegakan diagnosis. Pemeriksaan baku emas untuk tuberkulosis adalah kultur Lowenstein-Jensen yang membutuhkan waktu cukup lama untuk pertumbuhan koloni bakteri. Objektif: Penelitian ini bertujuan untuk mengetahui sensitivitas dan spesifisitas dari RT PCR menggunakan kit RT PCR yang bermerk Kit Crown Lab TB Dx dibandingkan dengan Kultur Lowenstein-Jensen. Me

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Umar, A., J. A. Hamza, Mujahid H., and P. Igba. "Comparative Studies of the Methods of Detecting Rifampicin Resistant Mycobacterium tuberculosis among Tuberculosis Patients Attending Aminu Kano Teaching Hospital, Kano." UMYU Journal of Microbiology Research (UJMR) 6, no. 2 (2021): 19–23. http://dx.doi.org/10.47430/ujmr.2162.003.

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This study was conducted to compare the specificity and sensitivity of GeneXpert MTB/Rif and Lowenstein-Jensen (LJ) Proportion methods of detecting rifampicin-resistant Mycobacterium tuberculosis among Acid Fast Bacilli (AFB) positive patients attending the Directly Observed Treatment (DOT) Centre of Aminu Kano Teaching Hospital Kano. A total of 150 AFB positive samples were collected and processed according to the guideline given by National TB and Leprosy Control Program (2015) and WHO (2012), The result revealed that rifampicin-resistant Mycobacterium tuberculosis (RR-TB) from the samples w

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Tortoli, Enrico, Paola Cichero, M. Gabriella Chirillo, et al. "Multicenter Comparison of ESP Culture System II with BACTEC 460TB and with Lowenstein-Jensen Medium for Recovery of Mycobacteria from Different Clinical Specimens, Including Blood." Journal of Clinical Microbiology 36, no. 5 (1998): 1378–81. http://dx.doi.org/10.1128/jcm.36.5.1378-1381.1998.

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The recently developed ESP Culture System II (AccuMed, Chicago, Ill.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-Jensen medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tuberculosis complex, followed by 37 isolates of theMycobacterium avium complex (MAC) and 53 isolates of eight other mycobacterial species. Rates of recovery obtained with BACTEC, ESP, and Lowenstein-Jensen med

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Susanty, Elva, Zainuddin Amir, Parluhutan Siagian, Rina Yunita, and Putri Chairani Eyanoer. "Uji Diagnostik Genexpert Mtb/Rif Di Rumah Sakit Umum Pusat Haji Adam Malik Medan." JURNAL BIOSAINS 1, no. 2 (2016): 19. http://dx.doi.org/10.24114/jbio.v1i2.2783.

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Background: Cases of multidrug resistant tuberculosis (MDR TB) is increasing in number in the world and requires early detection to prevent further transmission. GeneXpert MTB/RIF is a tool that can be used for detection of rifampicin resistance, as a surrogate marker for MDR TB. This study aims to assess the sensitivity and specificity of the GeneXpert MTB/RIF in diagnosis of MDR TB. Methods: diagnostic test study was conducted at a poly MDR TB General Hospital Haji Adam Malik Medan. The subjects were all suspected MDR TB who had results positive GeneXpert MTB/ RIF with sensitive rifampin or

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6

Asmar, S., S. Chatellier, C. Mirande, et al. "A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens." Journal of Clinical Microbiology 53, no. 8 (2015): 2566–69. http://dx.doi.org/10.1128/jcm.01149-15.

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The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causativeMycobacterium tuberculosisbacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21M. tuberculosisisolates at 105, 103, and 10 CFU yielded colonies in 5.7 ± 1.5 days, 7.6 ± 0.8 days, and 10.8 ± 1.7 days versus 1.5 ± 0.4 days, 3.5 ± 0.6 days, and 4.9 ± 1 days in MOD9 (P< 0.05, Student'sttest). Further, the

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Lyamin, Artem Viktorovich, Maxim Olegovich Zolotov, Andrei Vladimirovich Kozlov, Danir Damirovich Ismatullin, Tatyana Rudolfovna Nikitina, and Alexander Mikhailovich Kovalyov. "Comparison of Protein Profiles Obtained by Matrix-assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry in Representatives of the Family Mycobacteriaceae Grown on Various Nutrient Media." International Journal of Mycobacteriology 12, no. 3 (2023): 305–9. https://doi.org/10.4103/ijmy.ijmy_137_23.

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Abstract Background: The nutrient medium effects on the quality of the matrix-assisted laser desorption/ionization–time-of-flight (MALDI-ToF) mass spectra. The standard library includes spectra of microorganisms of the family Mycobacteriaceae grown on the Lowenstein–Jensen and Middlebrook Media. There are new methods for culturing microorganisms from this group, including inoculation on chromogenic media. Methods: The study included 240 strains of NTM isolated from patients during tuberculosis examination. The inoculation of the biological material was carried out on solid culture media of Low

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JO., Ajobiewe, Yashim NA., Sidi II, et al. "Level of Correlation of Opportunistic Mycobacterium Spp. Infection with Western Blot Confirmed Positive HIV-1 Infection in GOPD and S.T.C. Patients Attending National Hospital Abuja F.C.T. Nigeria." Scholars Journal of Applied Medical Sciences 9, no. 9 (2021): 1362–66. http://dx.doi.org/10.36347/sjams.2021.v09i09.009.

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The sole aim of this study is to monitor the level of correlation between GOPD and STC Patients whose samples (sera and sputa) were tested and confirmed with Western Blot, for HIV-1 and Ziehl Nielsen, Z N Stain/ Lowenstein Jensen, L J slope culture for Sputa respectively. These samples were screened for HIV- 1 using three test principles- Determine, Capillus and Genie. Positive samples were confirmed with the Western Blot assay. Sputa samples of the same patients were collected and tested for Mycobacterium spp. infection using the Ziehl Nielsen staining technique and the Lowenstein Jensen Slop

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Cortez, Marcio Valle, Cintia Mara Costa de Oliveira, Rossicléia Lins Monte, et al. "HIV-associated tuberculous lymphadenitis: the importance of polymerase chain reaction (PCR) as a complementary tool for the diagnosis of tuberculosis - a study of 104 patients." Anais Brasileiros de Dermatologia 86, no. 5 (2011): 925–31. http://dx.doi.org/10.1590/s0365-05962011000500010.

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BACKGROUND: Lymphadenitis is common in HIV-positive patients. Diagnosis of the infections associated with this condition is complex, particularly in the case of tuberculosis. Rapid and specific detection of Mycobacterium tuberculosis (M. tuberculosis) is fundamental in ensuring adequate treatment. In addition, frequent causes of lymphadenitis such as those associated with lymphoma and histoplasmosis, among others, must be eliminated as possible causes. OBJECTIVES: To evaluate the accuracy of polymerase chain reaction as a tool for the diagnosis of lymphadenitis resulting from M. tuberculosis.

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Presialia, Athira, and Ariyani Kiranasari. "Perbandingan Tingkat Kontaminasi Kultur Mycobacterium tuberculosis pada Medium Lowenstein-Jensen dan BACTEC MGIT 960." Jurnal Kedokteran Meditek 26, no. 2 (2020): 36–42. http://dx.doi.org/10.36452/jkdoktmeditek.v26i2.1829.

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Sampai saat ini, kultur masih merupakan standar pemeriksaan diagnosis tuberkulosis. Metode yang sering dilakukan adalah menggunakan Medium Lowenstein-Jensen dan BACTEC MGIT 960, namun kontaminasi oleh bakteri lain maupun jamur dapat terjadi dan dapat mengganggu hasil interpretasi. Penelitian ini bertujuan untuk mengetahui perbandingan tingkat kontaminasi kultur Mycobacterium tuberculosis pada Medium Lowenstein-Jensen dan BACTEC MGIT 960. Penelitian ini menggunakan desain studi potong lintang dengan 204 sampel dari Laboratorium Mikrobiologi Klinik FKUI-RSCM yang memenuhi kriteria penelitian. Da

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Yu, X., G. Jiang, H. Li, et al. "Rifampin Stability in 7H9 Broth and Lowenstein-Jensen Medium." Journal of Clinical Microbiology 49, no. 3 (2010): 784–89. http://dx.doi.org/10.1128/jcm.01951-10.

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Kaswabuli, Sylvia, Emmanuel Musisi, Patrick Byanyima, et al. "Accuracy of GenoQuick MTB test in detection of Mycobacterium tuberculosis in sputum from TB presumptive patients in Uganda." SAGE Open Medicine 10 (January 2022): 205031212211168. http://dx.doi.org/10.1177/20503121221116861.

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Objective: The objective of the study was to determine the diagnostic performance of the GenoQuick MTB test on heated sputum against the conventional Lowenstein–Jensen Mycobacterium tuberculosis culture as the reference method for tuberculosis diagnosis. Introduction: Fast, reliable, and easy-to-use tests for tuberculosis diagnosis are essential to achieving the Sustainable Development Goal of diagnosing and treating 90% of tuberculosis patients by 2030. We evaluated the diagnostic performance of the GenoQuick MTB, a polymerase chain reaction–lateral flow test, in Uganda, a resource-constraine

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S., Vanathi, Hemalatha R., Umamaheswari, Lallitha S., and Swarna S. "Comparative Study on Isolation of Mycobacterium Tuberculosis in Lowenstein Jensen Medium and Middle Brook 7H9 Broth from Sputum Samples of Suspected Cases of Pulmonary Tuberculosis and their Molecular Characterization by CBNAAT in a Tertiary Care Hospital, Thanjavur." International Journal of Pharmaceutical and Clinical Research 16, no. 8 (2024): 642–47. https://doi.org/10.5281/zenodo.13388277.

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Tuberculosis is caused by <em>Mycobacterium tuberculosis</em>, an acid-fast bacillus. The most common form of TB is pulmonary tuberculosis and can also cause extra pulmonary Tuberculosis. Nowadays we are facing the co-infection with HIV and the emergence of MDR-TB, XDR-TB. In developing countries like India, it causes major public health problem. The delay in diagnosis and the improper treatment are the important causes of mortality. In India, in our health care setup we mainly depend upon the Sputum Microscopy. The sputum microscopy and culture both of the diagnostic methods are very imp

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Nurdin, Gaby Maulida, and Irnayanti Bahar. "Skrining Antituberkulosis Ekstrak Tanaman Obat Lokal terhadap Mycobacterium tuberculosis Galur h37rv dan he dengan Metode Lowenstein-Jensen." CELEBES BIODIVERSITAS : Jurnal Sains dan Pendidikan Biologi 3, no. 1 (2020): 6. http://dx.doi.org/10.51336/cb.v3i1.200.

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Tuberkulosis (TB) menjadi masalah utama di negara berkembang termasuk Indonesia. Hal ini disebabkanoleh peningkatan kasus multidrug-resistant (MDR) dan extensively drug-resistant (XDR) pada strain Mycobacterium. Pengembangan senyawa aktif dari tanaman obat terus digalakkan. Penelitian ini bertujuanuntuk menyeleksi (skrining) antituberkulosis tanaman obat lokal terhadap pertumbuhan Mycobacteriumtuberculosis (Mtb) galur H37Rv dan HE dengan metode LJ (Lowenstein-Jensen). Sampel penelitian yangdigunakan berupa tanaman obat yang diduga berpotensi sebagai antituberkulosis, diantaranya mengkudu(Morin

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BUKHARI, AA, MSN KHAN, A. AKRAM, AA BUKHARI, N. NAEEM, and A. NAZ. "RETROSPECTIVE STUDY EVALUATING THE EFFICACY OF LJ MEDIUM ALONG WITH PENICILLIN TO IMPROVE CULTURE YIELD OF MYCOBACTERIUM TUBERCULOSIS IN PATIENTS." Biological and Clinical Sciences Research Journal 2023, no. 1 (2023): 327. http://dx.doi.org/10.54112/bcsrj.v2023i1.327.

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This study evaluated the efficacy of penicillin in improving the culture yield of M. Tuberculosis in cases of pulmonary tuberculosis. A retrospective study was conducted in the Department of Pathology, MMDC, Multan. A total of 350 suspected pulmonary tuberculosis patients were selected for the study. An early morning sputum specimen was collected. NAOH citrate and N-acetyl-L-cysteine were used for digesting, decontaminating, and homogenizing the sputum. The samples were processed on Lowenstein Jensen media and then Lowenstein Jensen media with penicillin. The cultures were then examined after

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Rahman, Fahmida, Sadia Sharmin, Md Mustafa Kamal, and Md Ruhul Amin Miah. "Drug resistance pattern of M. tuberculosis in category II treatment failure pulmonary tuberculosis patients." Ibrahim Medical College Journal 7, no. 1 (2014): 9–11. http://dx.doi.org/10.3329/imcj.v7i1.17741.

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This study was designed to determine the extent of drug resistance of M. tuberculosis (MTB) isolated from category II treatment failure pulmonary tuberculosis (PTB) patients. A total of 100 Ziehl-Neelsen (Z-N) smear positive category II failure PTB patients were included in this study. Sputum culture was done in Lowenstein-Jensen (L-J) media. Conventional proportion method on Lowenstein-Jensen (L-J) media was used to determine the drug susceptibility of M. tuberculosis to isoniazid (INH), rifampicin (RMP), ofloxacin (OFX) and kanamycin (KA). Out of 100 sputum samples, a total of 87 samples wer

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Tortoli, Enrico, Paola Cichero, Claudio Piersimoni, M. Tullia Simonetti, Giampietro Gesu, and Domenico Nista. "Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study." Journal of Clinical Microbiology 37, no. 11 (1999): 3578–82. http://dx.doi.org/10.1128/jcm.37.11.3578-3582.1999.

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The BACTEC MGIT 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Its performance was compared to those of the radiometric BACTEC 460 instrument and egg-based Lowenstein-Jensen medium. An identical volume of sample was inoculated in different media, and incubation was carried out for 6 weeks with the automatic systems and for 8 weeks on solid media. A total of 2,567 specimens obtained from 1,631 patients were cultured in parallel. Mycobacteria belonging to nine different taxa were isolated by at least one

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Annapurna, M. Mathrusri, M. E. Bhanoji Rao, and B. V. V. Ravi Kumar. "Synthesis, Spectral Characterization and Evaluation of Pharmacodynamic Activity of Copper and Nickel Complexes of Ethambutol Dihydrochloride." E-Journal of Chemistry 3, no. 4 (2006): 274–77. http://dx.doi.org/10.1155/2006/476513.

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Nickel and copper complexes of ethambutol [EB] were synthesized and characterized. On the basis of spectral and thermal data an octahedral geometry was assigned to the complexes with molecular formulae [Cu(C10H24N2O2)2Cl2] 5H2O and [Ni(C10H24N2O2)2Cl2] 2H2O. Coordination of ethylene diamine derivative to the metal ion was proposed. The anti-tubercular activity of the complexes was evaluated using Lowenstein–Jensen medium and MABA [Microplate Alamar Blue Assay] methods.

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Silva, Wellington Erasmo Lima e., Gisele Veneroni Gouveia, Maria da Conceição Aquino de Sá, et al. "Bacteria isolated from abscesses of small ruminants inspected in the semiarid region of Brazil." Semina: Ciências Agrárias 37, no. 3 (2016): 1337. http://dx.doi.org/10.5433/1679-0359.2016v37n3p1337.

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Loss in the supply chain of small ruminants owing to condemnations of carcasses in the abattoirs and slaughterhouses is common in northeastern Brazil. This study aims to identify bacterial agents, including Mycobacterium spp., in the abscesses found in the postmortem analysis of the carcasses of sheep and goats bred in northeastern Brazil. Our analysis involved 679 goats and 1,838 sheep carcasses. Abscess samples were extracted and inoculated on blood agar and Lowenstein Jensen with pyruvate or glycerol for bacterial isolation. We then performed polymerase chain reaction of the hps 65 gene; sa

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Kassaza, K., P. Orikiriza, A. Llosa, et al. "Lowenstein-Jensen Selective Medium for Reducing Contamination in Mycobacterium tuberculosis Culture." Journal of Clinical Microbiology 52, no. 7 (2014): 2671–73. http://dx.doi.org/10.1128/jcm.00749-14.

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Stewart, Linda D., Lyanne McCallan, James McNair, et al. "Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens." Journal of Clinical Microbiology 55, no. 12 (2017): 3411–25. http://dx.doi.org/10.1128/jcm.00728-17.

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ABSTRACTA novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific forMycobacterium bovisandM.capraecells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotyping or quantitative PCR [qPCR]) in each laboratory; liquid (MGIT or BacT/Alert) and/or solid (Stonebrink, Coletsos, or Lowenstein-Jensen) cultures were tested. In comparison to spoligotyping of acid-fast-positive MGIT cultures, percent agreement between positive LFD and spoligot

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Mardining Raras, Tri Yudhani, Almira Fahrinda,, Yuliati Yuliati, Dwi Yuni Nurhidayati, Hidayat Sujuti, and Sumarno Reto Prawiro. "IMMUNE RESPONSE ON THE ADMINISTRATION OF RECOMBINANT PROTEIN ANTIBODIES AG-38 KDA MYCOBACTERIUM TUBERCULOSIS AND RIFAMPICIN EX-VIVO." African Journal of Infectious Diseases 16, no. 2 (2022): 71–79. http://dx.doi.org/10.21010/ajid.v16i2.8.

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Background: Development a granuloma model resembling latent tuberculosis in vitro is needed with a fast and efficient time to be used as an effective therapy. This study aimed to form efficient granulomas, increase cellular immunity and humoral immunity, and evaluate growth on media using recombinant protein antibody Ag38kDa, Rifampicin, and a combination of both. Peripheral Blood Mononuclear Cell (PBMC) in vitro is derived from a healthy individual separated from monocytes and lymphocytes. Materials and methods: Monocytes are matured into macrophages and then combined macrophages and lymphocy

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Cvetkovikj, Iskra, Slavcho Mrenoshki, Kiril Krstevski, et al. "Bovine Tuberculosis in the Republic of Macedonia: Postmortem, Microbiological and Molecular Study in Slaughtered Reactor Cattle." Macedonian Veterinary Review 40, no. 1 (2017): 43–52. http://dx.doi.org/10.1515/macvetrev-2016-0097.

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Abstract Bovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonotic potential. The main purpose of the study was to determine the presence and distribution of the tuberculous lesions in reactor cattle, and to isolate and identify the causative agents of bovine tuberculosis in the Republic of Macedonia. Lymph nodes and affected organs from 188 reactor catt

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Wang, Chun Fang, Hua Rui Qi, Xiu Yun Jiang, et al. "Molecular Differentiation of Nontuberculous Mycobacterium Isolated from Different Animals." Applied Mechanics and Materials 421 (September 2013): 300–303. http://dx.doi.org/10.4028/www.scientific.net/amm.421.300.

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To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Lowenstein-Jensen medium and Middlebrook 7H9 agar, and then identified by nacterial smear, sequencing of16SrDNAand the 65-kDa heat-shock protein gene (hsp65 gene). The most frequently isolated species was Mycobacterium fortuitum. The result demonstrated that animals

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Tiamiyu, Abdulwasiu Bolaji, Garba Iliyasu, Farouq Muhammad Dayyab, et al. "Evaluation of GeneXpert MTB/RIF as a diagnostic tool in patients with sputum smear-negative TB in a high HIV burden region in Nigeria." Transactions of The Royal Society of Tropical Medicine and Hygiene 114, no. 9 (2020): 697–99. http://dx.doi.org/10.1093/trstmh/traa019.

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Abstract Background There are challenges in the diagnosis of TB in people with smear-negative pulmonary TB (SNPTB) in resource-limited settings. We evaluated the diagnostic usefulness of Xpert MTB/RIF compared with TB culture among SNPTB. Methods The study was a cross-sectional study among patients with SNPTB. The Xpert MTB/RIF tests and sputum culture (using Lowenstein-Jensen medium) were performed. Sensitivity and specificity were calculated. Results Of 150 patients studied, the sensitivity and specificity of GeneXpert MTB/RIF were 81.8 and 97.4%, respectively. Conclusion The sensitivity and

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Rasheed, M. U., and Getachew Belay. "Nocardiosis in HIV seropositive clinically suspected pulmonary tuberculosis patients." Tropical Doctor 38, no. 1 (2008): 34–35. http://dx.doi.org/10.1258/td.2007.060055.

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To determine the frequency of nocardiosis in HIV-positive individuals clinically suspected of having tuberculosis (TB), 140 sputum samples were collected and processed by Gram stain, modified Ziehl-Neelsen staining and by culture on Lowenstein Jensen medium. Four (2.85%) patients were positive for nocardia by microscopy and five (3.6%) had positive culture for Nocardia asterioides. In areas where HIV-associated TB is common, some patients diagnosed as smear-negative pulmonary TB will actually have nocardiosis. Clinicians should be aware of this entity in HIV/immunocompromised patients with res

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Saadatmand, Babak, James Kevin Poulton, and Catharine Lisa Kauffman. "Mycobacterium Marinum with Associated Bursitis." Journal of Cutaneous Medicine and Surgery 3, no. 4 (1999): 218–20. http://dx.doi.org/10.1177/120347549900300413.

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Background: Mycobacterium marinum infections have been reported for over 50 years, mostly in association with trauma in the setting of water exposure. Objective: The differential diagnosis for nodules in a sporotrichoid distribution with simultaneous bursitis is discussed. Mycobacterium marinum treatment regimens for skin and joint involvement are reviewed. Methods: Mycobacterium marinum was identified by skin tissue culture with Lowenstein-Jensen medium at 32°C. Histopathologic findings support mycobacterial infection. Results: Bursitis and nodules resolved in the first 2 months of a 6-month

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Misnarliah and Andi Ernawati. "EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEH HIJAU TERHADAP BAKTERI MYCOBACTERIUM TUBERCULOSIS." Jurnal Biogenerasi 10, no. 1 (2024): 160–65. https://doi.org/10.30605/biogenerasi.v10i1.4431.

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Tuberculosis is still a global health problem. Prolonged treatment of tuberculosis and using several anti-tuberculosis drugs (OAT) can cause side effects, one of which is multidrug resistance. Cases of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium strains continue to increase. . Research and development of active compounds from medicinal plants to achieve more effective tuberculosis treatment is still being promoted. This research aims to determine the potential and effectiveness of green tea plant (Camellia sinensis) leaf extract in inhibiting the growth of Myco

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Permatasari, Silvani, Vani Vrenika, Florence Felicia, et al. "Validitas Metode Real Time PCR GeneXpert pada Suspek TB Paru BTA Negatif di RSUD Dr. Doris Sylvanus." Jurnal Surya Medika 7, no. 1 (2021): 88–93. http://dx.doi.org/10.33084/jsm.v7i1.2037.

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Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Indonesia is one of the developing countries with high TB patients in the world. The most common detection used for TB diagnosis is sputum smear examination. Smear negative pulmonary TB still have a risk of infection and can develop into active. The GeneXpert molecular examination is a rapid diagnosis of TB by the real-time PCR method. The purpose of this study was to assess the validity of GeneXpert in smear-negative pulmonary TB compared to Lowenstein Jensen's culture. The design of this diagnostic test study is

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Aman, Munaza, Anjum Ara Mir, Gulnaz Bashir, Sayim Wani, Syed Khursheed, and Irfan Nisar Ahangar. "Evaluation of Colorimetric Nitrate Reductase Assay in Liquid Medium for Detection of Resistance to First-line Antitubercular Drugs." International Journal of Mycobacteriology 13, no. 2 (2024): 191–96. http://dx.doi.org/10.4103/ijmy.ijmy_69_24.

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Background: On a global scale, India holds the distinction of having the greatest number of tuberculosis (TB) cases caused by Mycobacterium tuberculosis (MTB) complex. The study aimed at evaluating the sensitivity, specificity, accuracy, cost, rapidity, and feasibility of the performance of the colorimetric nitrate reductase-based antibiotic susceptibility (CONRAS) test against the indirect proportion method (IPM) on Lowenstein–Jensen media as the gold standard. Methods: A comparative cross-sectional study was performed on 51 MTB isolates. Fresh subcultures were used for drug susceptibility te

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Anargyros, P., D. S. Astill, and I. S. Lim. "Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens." Journal of Clinical Microbiology 28, no. 6 (1990): 1288–91. http://dx.doi.org/10.1128/jcm.28.6.1288-1291.1990.

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Essa, Sherif Ahmed, Sohair Abdel-Rahman Abdel-Samea, Yasser Mahmoud Ismaeil, and Ahmad Abdelsadek Mohammad. "Comparative study between using Lowenstein Jensen and Bio-FM media in identification of Mycobacterium tuberculosis." Egyptian Journal of Chest Diseases and Tuberculosis 62, no. 2 (2013): 249–55. http://dx.doi.org/10.1016/j.ejcdt.2013.05.009.

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Abu Amero, K. K., and M. A. Halablab. "Evaluation of the COBAS AMPLICOR MTB test for the detection of Mycobacterium tuberculosis complex." Eastern Mediterranean Health Journal 10, no. 3 (2004): 329–35. http://dx.doi.org/10.26719/2004.10.3.329.

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Wevaluated the COBAS AMPLICOR polymerase chain reaction [PCR] based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. Acid-fast staining and culture on Lowenstein-Jensen medium were also performed on all samples. Of the 866 samples tested, 87 [10.0%] were PCR-positive compared to 94 [10.9%] culture positive. There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. A PCR inhibitory rate of 2.0% [17/866] was observed in respiratory samp

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Faburay, Alieu K., Francis S. Mendy, Jacob K. Otu, Tutty Isatou Faal-Jawara, F. Gehre, and Ousman Secka. "Performance comparison of a pair of Lowenstein–Jensen media supplemented with pyruvate or glycerol, and the combination of both supplements in a single Lowenstein–Jensen medium for the growth support of the Mycobacterium Tuberculosis complex." International Journal of Mycobacteriology 5 (December 2016): S169. http://dx.doi.org/10.1016/j.ijmyco.2016.09.019.

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Alam, Habiba Binte, Md Ruhul Amin Miah, Mostofa Kamal, Chandan Kumar Roy, and Ahmed Abu Saleh. "Thin-layer agar (TL7H11) for rapid isolation of Mycobacterium tuberculosis in sputum specimens." Bangabandhu Sheikh Mujib Medical University Journal 7, no. 1 (2016): 39. http://dx.doi.org/10.3329/bsmmuj.v7i1.29144.

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<p><strong>Background:</strong> Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. The early detection of new cases of pulmonary tuberculosis is an important goal in tuberculosis control program.</p><p><strong>Objective:</strong> 1n this study, thin layer agar (TLA) culture was compared with Lowenstein-Jensen (LJ) culture for rapid detection of pulmonary tuberculosis.<strong> </strong></p><p><strong>Methods:</strong> It was a cross sectional study conducted in Nationa

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Ghaleb, Khaled Ismail, Magdy Mohamed Afifi, and Mohamed Mohamed El Gohary. "ASSESSMENT OF DIAGNOSTIC TECHNIQUES OF URINARY TUBERCULOSIS." Mediterranean Journal of Hematology and Infectious Diseases 5, no. 1 (2013): e2013034. http://dx.doi.org/10.4084/mjhid.2013.034.

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Khalid Ghaleb a,* , Magdy Afifib, Mohamad El-Gohary c aDepartment of Medical Laboratories, Faculty of Applied Medical Science, King Khalid University, Bisha 551, Saudia Arabia bDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assuit 71524, Egypt cDepartment of Internal Medicine, Faculty of Medicine, Al-Azhar University, Assuit, Egypt The corresponding author e-mail: kh_ghaleb4@hotmail.com Current Tel: 00966595388496 Saudia, 00201119338055 Egypt The place of the study worked : Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assuit 7

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K., Mamuda, S. Olonitola O., D. Jatau E., and Nicholas E. "Evaluation of Nitrate Reductase Assay for Detection of Multi-drug Resistant Mycobacterium tuberculosis among Patients at National Tuberculosis Reference Laboratory Zaria Nigeria." Journal of Advances in Medical and Pharmaceutical Sciences 14, no. 1 (2017): 1–7. https://doi.org/10.9734/JAMPS/2017/33705.

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<strong>Aims: </strong>To evaluate nitrate reductase assay for detection of multi-drug resistant <em>Mycobacterium tuberculosis</em> among patients at National Tuberculosis Reference Laboratory Zaria Nigeria. <strong>Study Design:</strong> Hospital based cross sectional study <strong>Place and Duration of Study:</strong> National Tuberculosis Reference Laboratory Zaria Nigeria from December 2015 to June 2016. <strong>Methodology:</strong> A total of 437 re-treatment patients’ samples were screened for Acid Fast Bacilli (AFB), 72 were smear positive. Out of 72 smears positive, 62 were culture p

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Nikmawati, A., Windarwati Windarwati, and Hardjoeno Hardjoeno. "RESISTENSI Mycobacterium Tuberculosis TERHADAP OBAT ANTI TUBERKULOSIS." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 12, no. 2 (2018): 58. http://dx.doi.org/10.24293/ijcpml.v12i2.843.

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Patients infected with resistant Mycobacterium tuberculosis strain will be very difficult to cure by standard treatment. To evaluatethe drug resistance of Mycobacterium tuberculosis. A Cross sectional study was performed from January until July 2005. Samples wereobtained from sputum of the suspect tuberculosis. All samples were cultured in Lowenstein-Jensen Media and followed by sensitivity testaccording to resistance ratio method. Of 236 samples, there were 30% positive cultures. The percentage of mono-resistance to Isoniazidwas 70%, to Rifampicin 64.3%, to Ethambutol 62.8% and Streptomycin 6

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Narang, Rahul, Reema Ghodmare, and Pratibha Narang. "Evaluation of nitrate reductase assay on Lowenstein–Jensen medium for drug susceptibility testing of Mycobacterium tuberculosis." Journal of Mahatma Gandhi Institute of Medical Sciences 24, no. 2 (2019): 78. http://dx.doi.org/10.4103/jmgims.jmgims_59_16.

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Joseph, NoyalMariya, and Chettiyath Athira. "Evaluation of hypertonic saline–sodium hydroxide method for isolation of Mycobacterium tuberculosis on Lowenstein–Jensen medium." Journal of Current Research in Scientific Medicine 7, no. 2 (2021): 82. http://dx.doi.org/10.4103/jcrsm.jcrsm_6_21.

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Salfinger, M., and F. M. Kafader. "Comparison of two pretreatment methods for the detection of mycobacteria of BACTEC and Lowenstein-Jensen slants." Journal of Microbiological Methods 6, no. 6 (1987): 315–21. http://dx.doi.org/10.1016/0167-7012(87)90016-9.

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Worodria, William, Jillian Anderson, Adithya Cattamanchi, et al. "The Role of Speciation in Positive Lowenstein-Jensen Culture Isolates from a High Tuberculosis Burden Country." PLoS ONE 6, no. 11 (2011): e27017. http://dx.doi.org/10.1371/journal.pone.0027017.

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Shah, Sudeep R., Shubhada Shenai, Devendra C. Desai, Anand Joshi, Philip Abraham, and Camilla Rodrigues. "Comparison of Mycobacterium tuberculosis culture using liquid culture medium and Lowenstein Jensen medium in abdominal tuberculosis." Indian Journal of Gastroenterology 29, no. 6 (2010): 237–39. http://dx.doi.org/10.1007/s12664-010-0075-3.

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Armah, Zulfian, Rafika Rafika, Rahman Rahman, Arwin Arwin, Herdiana Herdiana, and Nurul Syafika. "AKTIVITAS PERTUMBUHAN Mycobacterium tuberculosis PADA MEDIA COCO BLOOD MALACHITE GREEN SEBAGAI ALTERNATIF PENGGANTI MEDIA LOWENSTEIN JENSEN." Jurnal Media Analis Kesehatan 14, no. 1 (2023): 43. http://dx.doi.org/10.32382/mak.v14i1.3329.

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Prasad, Ratna R., Choure Archana C, Wankhede Sachinkumar V, and Muley Vrishali A. "Subcutaneous Nocardiosis in a child with T- cell Immunodeficiency: A case report." IP International Journal of Medical Microbiology and Tropical Diseases 8, no. 1 (2022): 90–92. http://dx.doi.org/10.18231/j.ijmmtd.2022.019.

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Nocardia are weakly gram-positive, filamentous bacteria found worldwide in soils. Infection with Nocardia is rare inimmunocompetent patientsbut may leadto severe disease in immunocompromised patients. Reported cases in paediatric age group are few, and the literature is limited. We present a case of Nocardia isolated from pus from a skin lesion of eleven year old immunocompromised male child. Pus from Skin lesion on Ziehl-Neelsen (ZN) stain showed Acid Fast, Branching filamentous Bacteria. Pus culture on Lowenstein-Jensen (LJ) media showed glabrous, tough and waxycolonies. It was confirmed to

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Safitri, Ratu, Ida Indrawati, Mas Rizky A. A. Syamsunarno, Mohammad Ghozali, Basri A Gani, and Ramdan Panigoro. "ANTI-MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV AND IRON CHELATION ACTIVITY OF SAPPAN WOOD EXTRACT (CAESALPINIA SAPPAN L.) IN VITRO." Asian Journal of Pharmaceutical and Clinical Research 11, no. 6 (2018): 444. http://dx.doi.org/10.22159/ajpcr.2018.v11i6.25303.

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Objective: The objective of this study is to determine anti-Mycobacterium tuberculosis (MTB) strain H37Rv and iron chelation activities of sappan wood extract (SWE).Methods: The evaluation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by proportion methods. Whereas the iron chelate in Lowenstein–Jensen (LJ) medium as the indicator of Mycobacterium growth and SWE effect.Results: The SWE has bacteriocidal to MTB of 10−3 and 10−5 dilutions in of all concentrations (250, 500, 750, 1000, 2000, 4000, 8000, and 16000 part per millions [ppm]) also bacteriostati

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Pradhan, Prasil, Nimesh Poudyal, Rajendra Gurung, Anju Acharya, and Shyamal Kumar Bhattacharya. "Drug resistance pattern of Mycobacteria isolated from smear positive cases of pulmonary tuberculosis in eastern part of Nepal." Journal of General Practice and Emergency Medicine of Nepal 3, no. 4 (2014): 7–12. http://dx.doi.org/10.59284/jgpeman148.

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Introduction: The emergence of drug resistance in tuberculosis has become a major challenge for effective control of tuberculosis. This study was done with an aim to determine the drug resistance pattern of mycobacteria isolated from pulmonary tuberculosis. Methods: Sputum samples were collected from patients attending B.P. Koirala Institute of Health Sciences, Dharan during 1st June 2006 to 30th May 2007. The smear positive samples were cultured on Lowenstein Jensen medium and drug sensitivity was performed by proportion method. Results: Among 202 Mycobacterial isolates, 96 (47.52

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G. V. Coelho, Andréa, Liliana A . Zamarioli, Marilice P. Vicente, and Regina Ruivo Ferro e Silva. "Avaliação de método de Ogawa-Kudoh para o isolamento de micobactérias." Revista do Instituto Adolfo Lutz 58, no. 2 (1999): 57–61. http://dx.doi.org/10.53393/rial.1999.58.36694.

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O objetivo deste estudo foi avaliar o desempenho do método de descontaminação de Ogawa - Kudoh e a técnica de "swab"em meio de Ogawa (MOK), paralelamente à metodologia tradicional de Petroff em meio de Lowenstein - Jensen (MP) para o isolamento de micobactérias de espécimes clínicos de origem pulmonar e extrapulmonar. Foram analisadas 779 amostras de pacientes com suspeita clínica de tuberculose pulmonar e/ou micobacteriose. Destas, a positividade do MP foi de 13,96% e do MOK foi de 16,23% e a sensibilidade do MOK foi de 79% e a especificidade 94%. Observou-se ainda o crescimento das culturas

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Rohmi, Rohmi, Maruni Wiwin Diarti, and Yunan Jiwintarum. "Media Modifikasi Tepung Ikan Teri, Rumput Laut, Ubi Jalar Ungu dapat Mempercepat Pertumbuhan Mycobacterium Tuberculosis." Jurnal Kesehatan Manarang 7, no. 1 (2021): 7. http://dx.doi.org/10.33490/jkm.v7i1.312.

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Tuberculosis (TB) is one of the infectious diseases which is still a health problem in Indonesia caused by M. Tuberculosis. The common growth medium used to growth M. Tuberculosis is Lowenstein-Jensen. Examination by culture method has several disadvantages: the incubation period is too long to calculate the growth of M. tuberculosis that reaches 8 weeks to be able to declare negative results. LJ medium is relatively expensive and requires special manufacture that requires precision. Objective to know the result of growth rate and growth rate of M. tuberculosis from combination formulation med

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Nepali, S., P. Ghimire, DK Khadka, and S. Acharya. "Selective Inhibition of Mycobacterium tuberculosis by Para-Nitrobenzoic Acid (PNB) used in Lowenstein-Jensen Medium." SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS 5, no. 1 (2010): 25–28. http://dx.doi.org/10.3126/saarctb.v5i1.3080.

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Background: Mycobacterial growth in media to which inhibitory substances are added has been used in species identification. Mycobacterium tuberculosis does not grow in Lowenstein-Jensen (LJ) medium containing para-nitrobenzoic acid (PNB); which can be a basis for its identification from other mycobacteria. Setting: National Tuberculosis Centre (NTC), Thimi, Bhaktapur, Nepal. Objectives: To evaluate usefulness of PNB containing LJ medium in identifying mycobacterial isolates. Methodology: This diagnostic evaluation study based at NTC was conducted from Sep 2006-Jun 2007. During the study period

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