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Journal articles on the topic 'LptA'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

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1

Dai, Xiaowei, Min Yuan, Yu Lu, et al. "Identification of a Small Molecule That Inhibits the Interaction of LPS Transporters LptA and LptC." Antibiotics 11, no. 10 (2022): 1385. http://dx.doi.org/10.3390/antibiotics11101385.

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The need for novel antibiotics has become imperative with the increasing prevalence of antibiotic resistance in Gram-negative bacteria in clinics. Acting as a permeability barrier, lipopolysaccharide (LPS) protects Gram-negative bacteria against drugs. LPS is synthesized in cells and transported to the outer membrane (OM) via seven lipopolysaccharide transport (Lpt) proteins (LptA–LptG). Of these seven Lpt proteins, LptC interacts with LptA to transfer LPS from the inner membrane (IM) to the OM, and assembly is aided by LptD/LptE. This interaction among the Lpt proteins is important for the bi

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2

Sperandeo, Paola, Fion K. Lau, Andrea Carpentieri, et al. "Functional Analysis of the Protein Machinery Required for Transport of Lipopolysaccharide to the Outer Membrane of Escherichia coli." Journal of Bacteriology 190, no. 13 (2008): 4460–69. http://dx.doi.org/10.1128/jb.00270-08.

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ABSTRACT Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure and biosynthetic pathway are well known. Nevertheless, the mechanisms of transport and assembly of this molecule at the cell surface are poorly understood. The inner membrane (IM) transport protein MsbA is responsible for flipping LPS across the IM. Additional components of the LPS transport machinery downstream of MsbA have been identified, including the OM protein complex LptD/LptE (formerly Imp/RlpB), the periplasmic LptA protein, the IM-associated cytopla

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3

Vetterli, Stefan U., Katja Zerbe, Maik Müller, et al. "Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport inEscherichia coli." Science Advances 4, no. 11 (2018): eaau2634. http://dx.doi.org/10.1126/sciadv.aau2634.

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With the increasing resistance of many Gram-negative bacteria to existing classes of antibiotics, identifying new paradigms in antimicrobial discovery is an important research priority. Of special interest are the proteins required for the biogenesis of the asymmetric Gram-negative bacterial outer membrane (OM). Seven Lpt proteins (LptA to LptG) associate in most Gram-negative bacteria to form a macromolecular complex spanning the entire envelope, which transports lipopolysaccharide (LPS) molecules from their site of assembly at the inner membrane to the cell surface, powered by adenosine 5′-t

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4

Schultz, Kathryn M., Jimmy B. Feix, and Candice S. Klug. "Disruption of LptA oligomerization and affinity of the LptA-LptC interaction." Protein Science 22, no. 11 (2013): 1639–45. http://dx.doi.org/10.1002/pro.2369.

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5

Sperandeo, Paola, Rachele Cescutti, Riccardo Villa, et al. "Characterization of lptA and lptB, Two Essential Genes Implicated in Lipopolysaccharide Transport to the Outer Membrane of Escherichia coli." Journal of Bacteriology 189, no. 1 (2006): 244–53. http://dx.doi.org/10.1128/jb.01126-06.

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ABSTRACT The outer membrane (OM) of gram-negative bacteria is an asymmetric lipid bilayer that protects the cell from toxic molecules. Lipopolysaccharide (LPS) is an essential component of the OM in most gram-negative bacteria, and its structure and biosynthesis are well known. Nevertheless, the mechanisms of transport and assembly of this molecule in the OM are poorly understood. To date, the only proteins implicated in LPS transport are MsbA, responsible for LPS flipping across the inner membrane, and the Imp/RlpB complex, involved in LPS targeting to the OM. Here, we present evidence that t

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6

Martorana, Alessandra M., Mattia Benedet, Elisa A. Maccagni, et al. "Functional Interaction between the Cytoplasmic ABC Protein LptB and the Inner Membrane LptC Protein, Components of the Lipopolysaccharide Transport Machinery in Escherichia coli." Journal of Bacteriology 198, no. 16 (2016): 2192–203. http://dx.doi.org/10.1128/jb.00329-16.

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ABSTRACTThe assembly of lipopolysaccharide (LPS) in the outer leaflet of the outer membrane (OM) requires the transenvelope Lpt (lipopolysaccharide transport) complex, made inEscherichia coliof seven essential proteins located in the inner membrane (IM) (LptBCFG), periplasm (LptA), and OM (LptDE). At the IM, LptBFG constitute an unusual ATP binding cassette (ABC) transporter, composed by the transmembrane LptFG proteins and the cytoplasmic LptB ATPase, which is thought to extract LPS from the IM and to provide the energy for its export across the periplasm to the cell surface. LptC is a small

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7

Naclerio, George A., and Herman O. Sintim. "Multiple ways to kill bacteria via inhibiting novel cell wall or membrane targets." Future Medicinal Chemistry 12, no. 13 (2020): 1253–79. http://dx.doi.org/10.4155/fmc-2020-0046.

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The rise of antibiotic-resistant infections has been well documented and the need for novel antibiotics cannot be overemphasized. US FDA approved antibiotics target only a small fraction of bacterial cell wall or membrane components, well-validated antimicrobial targets. In this review, we highlight small molecules that inhibit relatively unexplored cell wall and membrane targets. Some of these targets include teichoic acids-related proteins (DltA, LtaS, TarG and TarO), lipid II, Mur family enzymes, components of LPS assembly (MsbA, LptA, LptB and LptD), penicillin-binding protein 2a in methic

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8

Myszka, Kamila, Natalia Tomaś, Łukasz Wolko, et al. "In situ approaches show the limitation of the spoilage potential of Juniperus phoenicea L. essential oil against cold-tolerant Pseudomonas fluorescens KM24." Applied Microbiology and Biotechnology 105, no. 10 (2021): 4255–68. http://dx.doi.org/10.1007/s00253-021-11338-3.

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Abstract The present study aimed to elucidate the effect of subinhibitory concentrations (sub-MICs) of juniper essential oil (EO), α-pinene, and sabinene on the quorum-sensing (QS)–mediated proteolytic and lipolytic properties of Pseudomonas fluorescens KM24. These activities were verified under in situ conditions, in which sub-MICs of the agents altered the morphology of KM24 cells. RNA-Seq studies revealed key coding sequences (CDSs)/genes related to QS and the proteolytic/lipolytic activities of pseudomonads. In this work, all the examined agents decreased autoinducer synthesis and influenc

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9

Hsueh, Yi-Ching, Eva-M. Brouwer, Julian Marzi, Oliver Mirus, and Enrico Schleiff. "Functional properties of LptA and LptD in Anabaena sp. PCC 7120." Biological Chemistry 396, no. 9-10 (2015): 1151–62. http://dx.doi.org/10.1515/hsz-2014-0322.

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Abstract Lipopolysaccharides (LPS) are central components of the outer membrane and consist of Lipid A, the core polysaccharide, and the O-antigen. The synthesis of LPS is initiated at the cytosolic face of the cytoplasmic membrane. The subsequent transport to and across the outer membrane involves multiple lipopolysaccharide transport (Lpt) proteins. Among those proteins, the periplasmic-localized LptA and the outer membrane-embedded LptD participate in the last steps of transfer and insertion of LPS into the outer membrane. While the process is described for proteobacterial model systems, no

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10

Romagnoli, P. "Serum (LPTS) and ascitic leptin (LPTA) levels in decompensated cirrhotic patients." Journal of Hepatology 34 (April 2001): 203. http://dx.doi.org/10.1016/s0168-8278(01)80747-9.

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11

Romagnoli, P., F. Botta, A. Fasoli, et al. "Serum (LPTS) and ascitic leptin (LPTA) levels in decompensated cirrhotic patients." Journal of Hepatology 34 (April 2001): 202. http://dx.doi.org/10.1016/s0168-8278(01)81622-6.

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12

Hicks, Greg, and Zongchao Jia. "Structural Basis for the Lipopolysaccharide Export Activity of the Bacterial Lipopolysaccharide Transport System." International Journal of Molecular Sciences 19, no. 9 (2018): 2680. http://dx.doi.org/10.3390/ijms19092680.

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Gram-negative bacteria have a dense outer membrane (OM) coating of lipopolysaccharides, which is essential to their survival. This coating is assembled by the LPS (lipopolysaccharide) transport (Lpt) system, a coordinated seven-subunit protein complex that spans the cellular envelope. LPS transport is driven by an ATPase-dependent mechanism dubbed the “PEZ” model, whereby a continuous stream of LPS molecules is pushed from subunit to subunit. This review explores recent structural and functional findings that have elucidated the subunit-scale mechanisms of LPS transport, including the novel AB

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13

Schultz, Kathryn M., Tanner J. Lundquist, Matthew A. Fischer, and Candice S. Klug. "LPS Binding to LptA." Biophysical Journal 112, no. 3 (2017): 446a. http://dx.doi.org/10.1016/j.bpj.2016.11.2390.

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14

Xiang, Quanju, Haiyan Wang, Zhongshan Wang, Yizheng Zhang, and Changjiang Dong. "Characterization of lipopolysaccharide transport protein complex." Open Life Sciences 9, no. 2 (2014): 131–38. http://dx.doi.org/10.2478/s11535-013-0250-5.

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AbstractLipopolysaccharide (LPS) is an essential component of the outer membranes (OM) of most Gram-negative bacteria, which plays a crucial role in protection of the bacteria from toxic compounds and harsh conditions. The LPS is biosynthesized at the cytoplasmic side of inner membrane (IM), and then transported across the aqueous periplasmic compartment and assembled correctly at the outer membrane. This process is accomplished by seven LPS transport proteins (LptA-G), but the transport mechanism remains poorly understood. Here, we present findings by pull down assays in which the periplasmic

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15

Schultz, Kathryn M., Matthew A. Fischer, Elizabeth L. Noey, and Candice S. Klug. "Disruption of the E. coli LptC dimerization interface and characterization of lipopolysaccharide and LptA binding to monomeric LptC." Protein Science 27, no. 8 (2018): 1407–17. http://dx.doi.org/10.1002/pro.3429.

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16

Takahashi, Hideyuki, Russel W. Carlson, Artur Muszynski, et al. "Modification of Lipooligosaccharide with Phosphoethanolamine by LptA in Neisseria meningitidis Enhances Meningococcal Adhesion to Human Endothelial and Epithelial Cells." Infection and Immunity 76, no. 12 (2008): 5777–89. http://dx.doi.org/10.1128/iai.00676-08.

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ABSTRACT The lipooligosaccharide (LOS) of Neisseria meningitidis can be decorated with phosphoethanolamine (PEA) at the 4′ position of lipid A and at the O-3 and O-6 positions of the inner core of the heptose II residue. The biological role of PEA modification in N. meningitidis remains unclear. During the course of our studies to elucidate the pathogenicity of the ST-2032 (invasive) meningococcal clonal group, disruption of lptA, the gene that encodes the PEA transferase for 4′ lipid A, led to a approximately 10-fold decrease in N. meningitidis adhesion to four kinds of human endothelial and

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17

Sperandeo, P., R. Villa, A. M. Martorana, et al. "New Insights into the Lpt Machinery for Lipopolysaccharide Transport to the Cell Surface: LptA-LptC Interaction and LptA Stability as Sensors of a Properly Assembled Transenvelope Complex." Journal of Bacteriology 193, no. 5 (2010): 1042–53. http://dx.doi.org/10.1128/jb.01037-10.

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18

Vrielink, Alice, Anandhi Anandan, Susannah Piek, Isabel Moares, and Charlene Kahler. "Structure of an endotoxin modifying enzyme and virulence factor in Neisseria." Acta Crystallographica Section A Foundations and Advances 70, a1 (2014): C1047. http://dx.doi.org/10.1107/s2053273314089529.

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Multiple drug resistance (MDR) in Gram-negative bacteria represents one of the most intractable problems facing modern medicine. Not only is antibiotic resistance incrementally increasing during clinical treatment of infections, but also the evolution and acquisition of new mechanisms of antibiotic resistance lead to the sudden loss of the capacity to treat infections. The most recent superbug, MDR-Neisseria gonorrhoeae, causes the untreatable sexually transmitted infection gonorrhoeae. Chronic gonococcal infections have a high morbidity rate and, due to the explosion in cases worldwide, the c

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19

Lewis, Lisa A., Biswa Choudhury, Jacqueline T. Balthazar, et al. "Phosphoethanolamine Substitution of Lipid A and Resistance of Neisseria gonorrhoeae to Cationic Antimicrobial Peptides and Complement-Mediated Killing by Normal Human Serum." Infection and Immunity 77, no. 3 (2008): 1112–20. http://dx.doi.org/10.1128/iai.01280-08.

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ABSTRACT The capacity of Neisseria gonorrhoeae to cause disseminated gonococcal infection requires that such strains resist the bactericidal action of normal human serum. The bactericidal action of normal human serum against N. gonorrhoeae is mediated by the classical complement pathway through an antibody-dependent mechanism. The mechanism(s) by which certain strains of gonococci resist normal human serum is not fully understood, but alterations in lipooligosaccharide structure can affect such resistance. During an investigation of the biological significance of phosphoethanolamine extensions

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20

Schultz, Kathryn M., and Candice S. Klug. "High-Pressure EPR Spectroscopy Studies of the E. coli Lipopolysaccharide Transport Proteins LptA and LptC." Applied Magnetic Resonance 48, no. 11-12 (2017): 1341–53. http://dx.doi.org/10.1007/s00723-017-0948-z.

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21

WRIGHT, Lesley C., Jackie PAYNE, Rosemary T. SANTANGELO, et al. "Cryptococcal phospholipases: a novel lysophospholipase discovered in the pathogenic fungus Cryptococcus gattii." Biochemical Journal 384, no. 2 (2004): 377–84. http://dx.doi.org/10.1042/bj20041079.

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The pathogenic fungus Cryptococcus neoformans produces an extracellular PLB1 (phospholipase B1), shown previously to be a virulence factor. A novel phospholipase (LPL1) with only LPL (lysophospholipase) and LPTA (transacylase) activities has now been characterized in C. gattii, and found to be a 66-kDa glycoprotein (by SDS/PAGE), with a native molecular mass of 670 kDa. The pI was 6.3, and it was active at high temperatures (to 70 °C), as well as at both acidic and neutral pH values. It was stimulated by calcium and palmitoyl carnitine at pH 7.0, but not at pH 5.0, and palmitoyl lysophosphatid

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22

Schultz, Kathryn M., Tanner J. Lundquist, and Candice S. Klug. "Lipopolysaccharide binding to the periplasmic protein LptA." Protein Science 26, no. 8 (2017): 1517–23. http://dx.doi.org/10.1002/pro.3177.

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23

CHEN, Sharon C. A., Lesley C. WRIGHT, John C. GOLDING, and Tania C. SORRELL. "Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans." Biochemical Journal 347, no. 2 (2000): 431–39. http://dx.doi.org/10.1042/bj3470431.

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Infection caused by the fungus Cryptococcus neoformans is potentially fatal. A highly active extracellular phospholipase, demonstrating phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities, was purified to homogeneity from C. neoformans using (NH4)2SO4 fractionation, and hydrophobic-interaction, anion-exchange and gel-filtration chromatography. All three enzyme activities co-purified as a single protein with an apparent molecular mass of 70-90 kDa by SDS/PAGE and 160-180 kDa by gel filtration. The ratio of the three activities remained constant af

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24

Zhang, Xuelian, Yan Li, Weiwei Wang, et al. "Identification of an anti-Gram-negative bacteria agent disrupting the interaction between lipopolysaccharide transporters LptA and LptC." International Journal of Antimicrobial Agents 53, no. 4 (2019): 442–48. http://dx.doi.org/10.1016/j.ijantimicag.2018.11.016.

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25

Merten, Jacqueline A., Kathryn M. Schultz, and Candice S. Klug. "Concentration-dependent oligomerization and oligomeric arrangement of LptA." Protein Science 21, no. 2 (2011): 211–18. http://dx.doi.org/10.1002/pro.2004.

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26

Shapiro, Adam B., Rong-Fang Gu, and Ning Gao. "Dimerization of isolated Pseudomonas aeruginosa lipopolysaccharide transporter component LptA." Biochemical and Biophysical Research Communications 450, no. 4 (2014): 1327–32. http://dx.doi.org/10.1016/j.bbrc.2014.06.138.

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27

Correia, Karoliny, and Aline Cassol Daga. "Compreensões sobre a formação para o/do ato de escrever na esfera acadêmica sob a perspectiva histórico-cultural." Revista Linguagem & Ensino 22, no. 3 (2019): 595. http://dx.doi.org/10.15210/rle.v22i3.16672.

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Este artigo discute processos de instrução e desenvolvimento de sujeitos em eventos com a escrita na esfera acadêmica na tentativa de compreender o percurso de formação para o/do ato de escrever. Os dados gerados correspondem à disciplina de Leitura e Produção Textual (LPTA) de uma universidade pública do sul do Brasil, tendo como participantes graduandas em Letras-Português e tutora a distância. Para a análise dos dados, adotou-se epistemologia analítica de base histórico-cultural, que tem como fundamento estudos bakhtinianos e vigotskianos com ênfase na educação em linguagem para a emancipaç

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Ganendren, Ranjini, Fred Widmer, Vatsala Singhal, Christabel Wilson, Tania Sorrell, and Lesley Wright. "In Vitro Antifungal Activities of Inhibitors of Phospholipases from the Fungal Pathogen Cryptococcus neoformans." Antimicrobial Agents and Chemotherapy 48, no. 5 (2004): 1561–69. http://dx.doi.org/10.1128/aac.48.5.1561-1569.2004.

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ABSTRACT Secreted phospholipase B is a proven virulence factor for the pathogenic fungus Cryptococcus neoformans and exhibits three phospholipase activities in the one protein. These are phospholipase B (PLB), lysophospholipase (LPL), and lysophospholipase transacylase (LPTA). Our aim was to investigate the feasibility of using this enzyme as a target for antifungal therapy. We determined in C. neoformans var. grubii strain H99 that 82% of PLB activity was secreted but that 64% of LPL activity and 70% of LPTA activity were cell associated. Cell-associated activities (cytosolic and membrane) we

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29

Baeta, Tiago, Karine Giandoreggio-Barranco, Isabel Ayala, et al. "The lipopolysaccharide-transporter complex LptB2FG also displays adenylate kinase activity in vitro dependent on the binding partners LptC/LptA." Journal of Biological Chemistry 297, no. 6 (2021): 101313. http://dx.doi.org/10.1016/j.jbc.2021.101313.

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Sulaiman, Luqman, Inga-Lena Nilsson, C. Christofer Juhlin, et al. "Genetic characterization of large parathyroid adenomas." Endocrine-Related Cancer 19, no. 3 (2012): 389–407. http://dx.doi.org/10.1530/erc-11-0140.

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In this study, we genetically characterized parathyroid adenomas with large glandular weights, for which independent observations suggest pronounced clinical manifestations. Large parathyroid adenomas (LPTAs) were defined as the 5% largest sporadic parathyroid adenomas identified among the 590 cases operated in our institution during 2005–2009. The LPTA group showed a higher relative number of male cases and significantly higher levels of total plasma and ionized serum calcium (P<0.001). Further analysis of 21 LPTAs revealed low MIB1 proliferation index (0.1–1.5%),MEN1mutations in five case

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31

Tzeng, Yih-Ling, Karita D. Ambrose, Susu Zughaier, et al. "Cationic Antimicrobial Peptide Resistance in Neisseria meningitidis." Journal of Bacteriology 187, no. 15 (2005): 5387–96. http://dx.doi.org/10.1128/jb.187.15.5387-5396.2005.

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ABSTRACT Cationic antimicrobial peptides (CAMPs) are important components of the innate host defense system against microbial infections and microbial products. However, the human pathogen Neisseria meningitidis is intrinsically highly resistant to CAMPs, such as polymyxin B (PxB) (MIC ≥ 512 μg/ml). To ascertain the mechanisms by which meningococci resist PxB, mutants that displayed increased sensitivity (≥4-fold) to PxB were identified from a library of mariner transposon mutants generated in a meningococcal strain, NMB. Surprisingly, more than half of the initial PxB-sensitive mutants had in

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Baysse, Christine, Méabh Cullinane, Valérie Dénervaud, et al. "Modulation of quorum sensing in Pseudomonas aeruginosa through alteration of membrane properties." Microbiology 151, no. 8 (2005): 2529–42. http://dx.doi.org/10.1099/mic.0.28185-0.

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Changes in the cellular envelope are major physiological adaptations that occur when micro-organisms encounter extreme environmental conditions. An appropriate degree of membrane fluidity is crucial for survival, and alteration of membrane lipids is an essential adaptive response. Emerging data suggest that microbial cells may recognize alterations in their membrane viscosity resulting from certain environmental changes as a trigger for adaptive cellular responses. In Pseudomonas aeruginosa, the quorum-sensing (QS) system involves a complex regulatory circuitry that coordinates the expression

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Widmer, Fred, Lesley C. Wright, Daniel Obando, et al. "Hexadecylphosphocholine (Miltefosine) Has Broad-Spectrum Fungicidal Activity and Is Efficacious in a Mouse Model of Cryptococcosis." Antimicrobial Agents and Chemotherapy 50, no. 2 (2006): 414–21. http://dx.doi.org/10.1128/aac.50.2.414-421.2006.

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ABSTRACT The alkyl phosphocholine drug miltefosine is structurally similar to natural substrates of the fungal virulence determinant phospholipase B1 (PLB1), which is a potential drug target. We determined the MICs of miltefosine against key fungal pathogens, correlated antifungal activity with inhibition of the PLB1 activities (PLB, lysophospholipase [LPL], and lysophospholipase-transacylase [LPTA]), and investigated its efficacy in a mouse model of disseminated cryptococcosis. Miltefosine inhibited secreted cryptococcal LPTA activity by 35% at the subhemolytic concentration of 25 μM (10.2 μg

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34

Handing, Jonathan W., and Alison K. Criss. "The lipooligosaccharide-modifying enzyme LptA enhances gonococcal defence against human neutrophils." Cellular Microbiology 17, no. 6 (2015): 910–21. http://dx.doi.org/10.1111/cmi.12411.

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Santambrogio, Carlo, Paola Sperandeo, Riccardo Villa, Frank Sobott, Alessandra Polissi, and Rita Grandori. "LptA Assembles into Rod-Like Oligomers Involving Disorder-to-Order Transitions." Journal of The American Society for Mass Spectrometry 24, no. 10 (2013): 1593–602. http://dx.doi.org/10.1007/s13361-013-0687-9.

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Juibari, Aref Doozandeh, Sina Ramezani, and Mohammad Hosein Rezadoust. "Bioinformatics analysis of various signal peptides for periplasmic expression of parathyroid hormone in E.coli." Journal of Medicine and Life 12, no. 2 (2019): 184–91. http://dx.doi.org/10.25122/jml-2018-0049.

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Hypoparathyroidism is a rare endocrine disease which is characterized by the deficiency of serum calcium levels. RhPTH is prescribed as a therapy for the management of refractory hypoparathyroidism. The aim of this study is to investigate 32 signal peptides of gram-negative bacterial origin and evaluate their potential for efficient secretion of recombinant human PTH (1–84)In E.coli to obtain higher expression of recombinant PTH in bacterial systems by using this fusion partner. SignalP and ProtParam servers were employed to predict the presence and location of signal peptide cleavage sites in

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Wang, Jia, Wen-Jun Liu, Hou-Zhen Shi, Hong-Ru Zhai, Jin-Jun Qian, and Wei-Ning Zhang. "A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease." Cells 11, no. 18 (2022): 2849. http://dx.doi.org/10.3390/cells11182849.

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Emerging evidence suggests that the proper control of mitochondrial dynamics provides a window for therapeutic intervention for Alzheimer’s disease (AD) progression. The transcriptional coactivator peroxisome proliferator activated receptor gamma coactivator 1 (PGC-1a) has been shown to regulate mitochondrial biogenesis in neurons. Thus far, the roles of PGC-1a in Alzheimer’s disease and its potential value for restoring mitochondrial dysfunction remain largely unknown. In the present study, we explored the impacts of PGC-1a on AD pathology and neurobehavioral dysfunction and its potential mec

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Uddin, Md Bashir, S. M. Bayejed Hossain, Mahmudul Hasan, et al. "Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken." Animals 11, no. 1 (2021): 206. http://dx.doi.org/10.3390/ani11010206.

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Colistin (polymyxin E) is widely used in animal and human medicine and is increasingly used as one of the last-resort antibiotics against Gram-negative bacilli. Due to the increased use of colistin in treating infections caused by multidrug-resistant Gram-negative bacteria, resistance to this antibiotic ought to be monitored. The study was undertaken to elucidate the molecular mechanisms, genetic relationships and phenotype correlations of colistin-resistant isolates. Here, we report the detection of the mcr-1 gene in chicken-associated Salmonella isolates in Bangladesh and its in-silico funct

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Wanty, Christopher, Anandhi Anandan, Susannah Piek, et al. "The Structure of the Neisserial Lipooligosaccharide Phosphoethanolamine Transferase A (LptA) Required for Resistance to Polymyxin." Journal of Molecular Biology 425, no. 18 (2013): 3389–402. http://dx.doi.org/10.1016/j.jmb.2013.06.029.

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Wenzel, Cory Q., Frank St. Michael, Jacek Stupak, Jianjun Li, Andrew D. Cox, and James C. Richards. "Functional Characterization of Lpt3 and Lpt6, the Inner-Core Lipooligosaccharide Phosphoethanolamine Transferases from Neisseria meningitidis." Journal of Bacteriology 192, no. 1 (2009): 208–16. http://dx.doi.org/10.1128/jb.00558-09.

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ABSTRACT The lipooligosaccharide (LOS) of Neisseria meningitidis contains heptose (Hep) residues that are modified with phosphoethanolamine (PEtn) at the 3 (3-PEtn) and/or 6 (6-PEtn) position. The lpt3 (NMB2010) and lpt6 (NMA0408) genes of N. meningitidis, which are proposed to encode the required HepII 3- and 6-PEtn transferases, respectively, were cloned and overexpressed as C-terminally polyhistidine-tagged fusion proteins in Escherichia coli and found to localize to the inner membrane, based on sucrose density gradient centrifugation. Lpt3-His6 and Lpt6-His6 were purified from Triton X-100

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Wright, J. Claire, Derek W. Hood, Gaynor A. Randle, et al. "lpt6, a Gene Required for Addition of Phosphoethanolamine to Inner-Core Lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae." Journal of Bacteriology 186, no. 20 (2004): 6970–82. http://dx.doi.org/10.1128/jb.186.20.6970-6982.2004.

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ABSTRACT We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the β-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (PEtn-6) on HepII. We have now identified a gene, lpt6 (NMA0408), that is required for the addition of PEtn-6 on HepII. The lpt6 gene is located in a region previously identified as Lgt-3 and is associated with other LPS biosynthetic genes. We screened 113 strains, representi

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Fu, Hai-Gen, Zhi-Wen Li, Xin-Xin Hu, et al. "Synthesis and Biological Evaluation of Quinoline Derivatives as a Novel Class of Broad-Spectrum Antibacterial Agents." Molecules 24, no. 3 (2019): 548. http://dx.doi.org/10.3390/molecules24030548.

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Nineteen new quinoline derivatives were prepared via the Mannich reaction and evaluated for their antibacterial activities against both Gram-positive (G+) and Gram-negative (G−) bacteria, taking compound 1 as the lead. Among the target compounds, quinolone coupled hybrid 5d exerted the potential effect against most of the tested G+ and G− strains with MIC values of 0.125–8 μg/mL, much better than those of 1. Molecular-docking assay showed that compound 5d might target both bacterial LptA and Top IV proteins, thereby displaying a broad-spectrum antibacterial effect. This hybridization strategy

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Zhu, Chuan, Yao Wang, Guangjie Han, Joel J. P. C. Rodrigues, and Jaime Lloret. "LPTA: Location Predictive and Time Adaptive Data Gathering Scheme with Mobile Sink for Wireless Sensor Networks." Scientific World Journal 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/476253.

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This paper exploits sink mobility to prolong the lifetime of sensor networks while maintaining the data transmission delay relatively low. A location predictive and time adaptive data gathering scheme is proposed. In this paper, we introduce a sink location prediction principle based on loose time synchronization and deduce the time-location formulas of the mobile sink. According to local clocks and the time-location formulas of the mobile sink, nodes in the network are able to calculate the current location of the mobile sink accurately and route data packets timely toward the mobile sink by

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Ma, B., C. M. Reynolds, and C. R. H. Raetz. "Periplasmic orientation of nascent lipid A in the inner membrane of an Escherichia coli LptA mutant." Proceedings of the National Academy of Sciences 105, no. 37 (2008): 13823–28. http://dx.doi.org/10.1073/pnas.0807028105.

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Mirbod, Fariba, Yoshiko Banno, Mahmoud A. Ghannoum, et al. "Purification and characterization of lysophospholipase-transacylase (h-LPTA) from a highly virulent strain of Candida albicans." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1257, no. 2 (1995): 181–88. http://dx.doi.org/10.1016/0005-2760(95)00072-k.

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Araújo, Júlio, Marcilene Gaspar Barros, and Elinaldo Soares Silva. "Práticas de reescrita no ensino do gênero resenha." Revista Brasileira de Linguística Aplicada 15, no. 1 (2015): 109–30. http://dx.doi.org/10.1590/1984-639820155541.

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A escrita de gêneros acadêmicos exige perícia e reflexão e deve se configurar como um processo constante de reescrita, marcado por muitos ajustes linguístico-textuais. Acreditando que a vivência desse processo pode ser ensinada, o objetivo deste artigo é refletir sobre a reescrita do gênero resenha por estudantes do curso de Letras da Universidade Federal do Ceará (UFC). A base teórica desse exercício de análise procede da perspectiva de reescrita proposta por Fabre-Cols (2002) e da teoria de gêneros de Swales (1984; 1990). À luz desses autores, analisamos as mudanças efetuadas em resenhas aca

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Trombley, Michael P., Deborah M. B. Post, Sherri D. Rinker, et al. "Phosphoethanolamine Transferase LptA in Haemophilus ducreyi Modifies Lipid A and Contributes to Human Defensin Resistance In Vitro." PLOS ONE 10, no. 4 (2015): e0124373. http://dx.doi.org/10.1371/journal.pone.0124373.

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Tran, An X., M. Stephen Trent, and Chris Whitfield. "The LptA Protein ofEscherichia coliIs a Periplasmic Lipid A-binding Protein Involved in the Lipopolysaccharide Export Pathway." Journal of Biological Chemistry 283, no. 29 (2008): 20342–49. http://dx.doi.org/10.1074/jbc.m802503200.

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Firoved, Aaron M., J. Cliff Boucher та Vojo Deretic. "Global Genomic Analysis of AlgU (σE)-Dependent Promoters (Sigmulon) in Pseudomonas aeruginosa and Implications for Inflammatory Processes in Cystic Fibrosis". Journal of Bacteriology 184, № 4 (2002): 1057–64. http://dx.doi.org/10.1128/jb.184.4.1057-1064.2002.

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ABSTRACT The conversion of Pseudomonas aeruginosa to the mucoid phenotype coincides with the establishment of chronic respiratory infections in cystic fibrosis (CF). A major pathway of conversion to mucoidy in clinical strains of P. aeruginosa is dependent upon activation of the alternative sigma factor AlgU (P. aeruginosa σE). Here we initiated studies of AlgU-dependent global expression patterns in P. aeruginosa in order to assess whether additional genes, other than those involved in the production of the mucoid exopolysaccharide alginate, are turned on during conversion to mucoidy. Using g

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Araújo, Júlio César, and Messias Dieb. "Interação virtual e a autoria de artigos científicos: nos bastidores da produção acadêmica." Educação em Revista 26, no. 3 (2010): 387–406. http://dx.doi.org/10.1590/s0102-46982010000300020.

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Este artigo trata de uma experiência com o gênero digital fórum educacional na disciplina Leitura e Produção do Texto Acadêmico (LPTA), do curso de Letras da UFC, cujo propósito foi desenvolver habilidades de compreensão e produção de textos acadêmicos, em especial o artigo científico. Para isso, os estudantes, em aulas presenciais, aprenderam sobre o gênero artigo científico e seus movimentos retóricos mais comuns. A partir dessas aulas, começaram a produzir seu próprio texto e, nesse momento, o fórum serviu como ferramenta didática para a apropriação de saberes em torno do artigo. Como resul

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