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Du, Yanhai. "Interactions and Compatibilities of LSGM Electrolyte and LSCM Anode." ECS Proceedings Volumes 2005-07, no. 1 (January 2005): 1127–36. http://dx.doi.org/10.1149/200507.1127pv.
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Zhou, Kunhua, Zhizheng Wu, Tianyu Zhang, Feng Li, Azhar Iqbal, and Suresh Sivanandam. "Active Aberration Correction with Adaptive Coefficient SPGD Algorithm for Laser Scanning Confocal Microscope." Sensors 22, no. 10 (May 15, 2022): 3755. http://dx.doi.org/10.3390/s22103755.
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A laser scanning confocal microscope (LSCM) is an effective scientific instrument for studying sub-micron structures, and it has been widely used in the field of biological detection. However, the illumination depth of LSCMs is limited due to the optical aberrations introduced by living biological tissue, which acts as an optical medium with a non-uniform refractive index, resulting in a significant dispersion of the focus of LSCM illumination light and, hence, a loss in the resolution of the image. In this study, to minimize the effect of optical aberrations, an image-based adaptive optics technology using an optimized stochastic parallel gradient descent (SPGD) algorithm with an adaptive coefficient is applied to the optical path of an LSCM system. The effectiveness of the proposed aberration correction approach is experimentally evaluated in the LSCM system. The results illustrate that the proposed adaptive optics system with an adaptive coefficient SPGD algorithm can effectively reduce the interference caused by aberrations during depth imaging.
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Yu, Jie, Wen Hui Ma, Hang Sheng Lin, Hong Yan Sun, Xiu Hua Chen, and Bin Yang. "Fabrication of LSGM Thin Film Electrolyte on LSCM Anode by RF Magnetron Sputtering for IT-SOFC." Materials Science Forum 675-677 (February 2011): 81–84. http://dx.doi.org/10.4028/www.scientific.net/msf.675-677.81.
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La0.9Sr0.1Ga0.8Mg0.2O3-δ (LSGM) thin film electrolytes were fabricated on La0.7Sr0.3Cr0.5Mn0.5O3-δ (LSCM) porous anodes by radio-frequency (RF) magnetron sputtering. The formation and microstructure of LSGM thin films were characterized by X-ray diffraction(XRD) and scanning electron microscopy (SEM). The effects of different sputtering conditions, such as Ar gas pressure, substrate temperature and sputtering power, on the performance of LSGM electrolyte film were estimated. Dense LSGM thin film electrolytes with thickness of about 2μm, which are compatible with LSCM-based anodes and without crack, have been successfully fabricated on LSCM-based anode supports by RF magnetron sputtering when sputtering power density is 5.2W·cm-2, Ar gas pressure is 5Pa and substrate temperature is 300°C. It is found that high sputtering power density and high Ar gas pressure, as well as high substrate temperature, are beneficial to deposition of dense electrolyte thin film, close bonding of electrolyte thin film with anode substrate, and formation of large three phase boundaries between anode and electrolyte.
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Aljawarneh, Nader Mohammad, Mohamad M. Taamneh, Nouh Alhndawi, Khaled Abed AlQader alomari, and Fawzieh Masa'd. "Fog computing-based logistic supply chain management and organizational agility: The mediating role of user satisfaction." Uncertain Supply Chain Management 9, no. 3 (2021): 767–78. http://dx.doi.org/10.5267/j.uscm.2021.4.001.
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Although fog computing-based logistic supply chain management (Fog computing-based LSCM) is an emerging technology that proved a high impact on services and products, little research has focused on fog computing-based LSCM. Drawing on the Kano model and organization's theory this paper investigates the effect of fog computing-based LSCM on organizational agility. And the role of user satisfaction as mediator between fog computing-based LSCM and organizational agility. A quantitative approach was used, a questionnaire was designed for data collection, Cronbach's Alpha test was performed on a pilot study to examine the internal consistency of questionnaire items. Fog computing-based LSCM was studied based on Supply chain awareness, Connectivity and Logistics, Integration Process, Seamless Supply Chain, Integration of Processes. Data was collected from a random sample of 550 employees of Al-Hassan industrial city in Jordan. Building on the proposed model, Researchers show that fog computing-based LSCM has a positive impact on organizational agility, fog computing-based LSCM has a positive impact on user satisfaction and finally user satisfaction mediates the relationship between fog computing-based LSCM and organizational agility. Implications for the model are discussed.
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Li, Rui, Xiu Hua Chen, Wen Hui Ma, Jie Xing, Jian Jun Yang, and Jie Yu. "Preparation and Characterization of NiO-LSCM/LSCM Dual Composite Anode for IT-SOFC." Advanced Materials Research 898 (February 2014): 128–31. http://dx.doi.org/10.4028/www.scientific.net/amr.898.128.
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The LSCM (La0.7Sr0.3Cr0.5Mn0.5O3-δ) powders were synthesized by glycine-nitrate process (GNP). An anode functional layer (AFL) LSCM was fabricated on an porous NiO-LSCM anode substrate by slurry spin coating. The effect of pore-former, sintering temperature and sintering time on the quality of thin film was investigated. The film with good apparent morphology was obtained when the operating parameters were fixed as follows: the pore-former is ethyl cellulose, the content of ethyl cellulose is 5 wt.%, the sintering temperature is 1000 °C and the sintering time is 4 h.
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JBR, Subarna Bir, and Umesh Singh Yadav. "A Fit between Logistics and Supply Chain Management Education and Jobs Market Need in Nepal." Journal of Nepalese Business Studies 14, no. 1 (December 20, 2021): 54–68. http://dx.doi.org/10.3126/jnbs.v14i1.41489.
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The purpose of this paper is to explore a fit between Logistics and Supply Chain Management (LSCM) related course content and the industry needs in the Nepalese context. Since this study is undertaken using the Nepalese sample, the knowledge and skills prioritized by employers, it can be of value to educators while designing their LSCM course content. Desk-based research involving content analyses was done to understand the supply side information i.e. relative coverage of LSCM topics in business-related courses and degrees offered at selected five Nepalese Universities and for the demand side information i.e. analysis of job description of the LSCM related vacancies in the Nepalese job market over eighteen weeks. The study reveals that the inclusion of LSCM courses in the business programs at Nepalese Universities is currently negligible as none of them offered a separate program dedicated to LSCM. Besides, the LSCM courses were limited to just one course per program weighing not more than three credit hours. Instead, there seems to be an unprecedented number of business schools and colleges leaning towards more sellable traditional business degrees related to finance, marketing, human resource management, IT, and hospitality. Finally, when comparing the relative coverage of LSCM topics in the curriculum to the relative demand for such knowledge by the employers, there seems to be an over-emphasis or under-emphasis of courses related to LSCM both at the bachelors and masters level indicating a mismatch between the expectations of employers and education offered by the universities.
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Merkert, Rico, Kai Hoberg, and Kumaraguru Mahadevan. "Logistics and Supply Chain Superpowers and Skills for Survival in the “New Normal” Globalized World." Transportation Journal 62, no. 4 (2023): 369–96. http://dx.doi.org/10.5325/transportationj.62.4.0369.
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Abstract Disruptions and rising costs in global supply chains due to COVID-19, climate change, and geopolitical tensions have led to supernormal profits, substantially more recognition of the importance of the sector and management functions associated with it, and a potential “golden age” for a career in logistics and supply chain management (LSCM). This article provides a general overview of the opportunities arising from global LSCM trends. Motivated by student views and comments, the authors have surveyed leading academics and senior practitioners globally across the key LSCM functions to obtain a comprehensive view on the future skills required to excel in a career in supply chain management. Their findings suggest a multitude of future-proof skills centred around technical (e.g., data analytics), soft (e.g., creativity) and cross-functional skills, which they then use to discuss implications for job profiles, graduates (i.e., learn to learn) and LSCM professionals. Given the variety and complexity of the identified skills and LSCM functions, in addition to a compounding LSCM talent shortage, the authors propose changes in LSCM offerings at universities and other tertiary education providers, with a focus on unlearning and life-long learning.
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Grant, David B., Sarah Shaw, Edward Sweeney, Witold Bahr, Siriwan Chaisurayakarn, and Pietro Evangelista. "Using mixed methods in logistics and supply chain management research: current state and future directions." International Journal of Logistics Management 34, no. 7 (November 10, 2023): 177–98. http://dx.doi.org/10.1108/ijlm-04-2023-0156.
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PurposeMixed methods research is useful to enhance theoretical and practical research contributions. However, single methods have predominated much logistics and supply chain management (LSCM) research. This paper presents a review of mixed methods research across ten years in LSCM to determine their usage, identify benefits and inhibitors, and provide suggestions for LSCM researchers to realise the benefits from using mixed methods.Design/methodology/approachThis paper adopts a mixed methods approach through a quantitative analysis of methods used in six leading LSCM journals, an e-mail survey of mixed methods article authors during the review period, and four published case studies that used mixed methods.FindingsOnly 144 (ten percent) of all empirical articles were published using mixed methods during the review period. A range of benefits and inhibitors regarding mixed methods adoption were found. Suggestions for LSCM authors include research training in mixed methods use and developing a project-specific research design due to the specificity and complexity associated with mixed methods research.Originality/valueLSCM is at a critical juncture, shaped by new contexts, themes and challenges, and would benefit from different research approaches and methods. This paper contributes to the LSCM domain through analysing the current state, benefits and inhibitors of mixed methods research in LSCM journals to provide a renewed call to action and guidelines for mixed methods LSCM research, and suggesting research design adaptation to enable agile and resilient research when investigating rapidly changing and complex phenomena.
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Tortorella, Guilherme Luz, Ricardo Giglio, and Jorge Limon-Romero. "Supply chain performance: how lean practices efficiently drive improvements." Journal of Manufacturing Technology Management 29, no. 5 (August 13, 2018): 829–45. http://dx.doi.org/10.1108/jmtm-09-2017-0194.
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Purpose The purpose of this paper aims at investigating which lean supply chain management (LSCM) practices efficiently improve the supply chain performance. Design/methodology/approach To achieve that, a cross-sectional survey was carried out with 113 manufacturing companies undergoing a lean implementation. The proposed method combines complementary methods of multivariate data analysis in order to determine which bundles of LSCM practices more efficiently entail improvements on supply chain performance. Findings The findings justify why some LSCM initiatives may find larger barriers than others, compromising their success due to misguided implementation efforts according to the desired performance improvement. Originality/value The empirical examination on the efficiency of LSCM practices with regards to a certain set of performance indicators provides guidelines with respect to LSCM implementation depending on which performance indicators are envisioned for improvement.
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El Jaouhari, Asmae, Soumaya Fellaki, Mohamed Amejwal, and Jabir Arif. "Industry 4.0 Implementation in Lean Supply Chain Management: a Systematic Literature Review." Statistics, Optimization & Information Computing 11, no. 1 (January 23, 2023): 178–85. http://dx.doi.org/10.19139/soic-2310-5070-1539.
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The major aspects and impacts of the interrelationships between Industry 4.0 (I4.0) technology and Lean Supply Chain Management are discussed in this article (LSCM). Many practical LSCM systems based on I4.0 have lately appeared. Despite this, there has been little research into the use of I4.0 technologies within LSCM. Machine learning, smart factories, blockchain, and the internet of services (IoS) are all possible LSCM revolution enablers. The goal of this research is to find out more about present and potential I4.0 technologies that can improve LSCM research and application in order to fill a gap in the current literature. A Systematic Literature Review (SLR) technique was used for the collection, selection, and evaluation of published literature. We looked at 79 studies published between 2010 and 2021 that were found in the Scopus database.
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-Duah, Gloria Pokuaa, and Devika Nadarajah. "Improving of Organizational Performance Using Lean Supply Chain Management Practices: The Mediating Role of Supply Chain Collaboration." Business Management and Strategy 12, no. 2 (June 24, 2021): 35. http://dx.doi.org/10.5296/bms.v12i2.18795.
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Purpose - Surviving in the face of growing globalization, competition and rapid changes in consumer expectations would mean that, firms have to adopt modern business strategies that are flexible and capable of sustaining performance improvements among firms. The aim of this paper is to establish the effect of lean supply chain management (LSCM) approach on performance improvement among firms through the influence of supply chain collaboration (SCC).Design/Approach/Methodology - This conceptual paper recommends an inclusive model that incorporate LSCM and SCC dimensions as well as organizational performance, underpinned by theories such as the Dynamic Capabilities (DCT) and Relational View (RV). The proposed model is to establish the direct and indirect effects of LSCM approach on firms’ performance through the influence of SCC (intra and inter firm) dimensions in the manufacturing context, as the main goal of LSCM implementation which will subsequently lead to improvements in organizational performance.Originality: The study effectively combines the DCT and RV to develop a comprehensive model involving the three broad operations and supply chain management concepts. Though there may be similar studies on LSCM practices among manufacturing firms, limited studies have explored similar model or work has limited discussion especially on exploring the mediating roles of individual dimensions of SCC in the LSCM practices – organizational performance among manufacturing firms. This is a novelty as it provides deeper insights on the mediating effects of each dimension of SCC in the relationship between LSCM practices and organizational performance. The proposed theoretical model will serve as a potential blueprint for the successful implementation of LSCM practices.
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Jasti, Naga Vamsi Krishna, and Suresh Kurra. "An empirical investigation on lean supply chain management frameworks in Indian manufacturing industry." International Journal of Productivity and Performance Management 66, no. 6 (July 10, 2017): 699–723. http://dx.doi.org/10.1108/ijppm-12-2015-0185.
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Purpose Supply chain management plays a vital role in deciding the final cost of products. A huge number of non-value-added activities are performed throughout supply chain process. Lean principles are useful to identify and eliminate waste activities across the supply chain management processes. The purpose of this paper is to examine the validity and reliability of the existing lean supply chain management (LSCM) frameworks in the Indian manufacturing industry through questionnaire survey methodology. Design/methodology/approach The present study collected empirical data from 180 top- and middle-level management personnel from the Indian manufacturing industry. Factor analysis was performed to check unidimensionality of LSCM frameworks by using empirical data. Cronbach’s α value of each selected LSCM framework was calculated to find out its reliability. Finally, frequency distribution analysis was performed on the selected framework to identify and reveal critical constructs of LSCM. Findings It was found that nine LSCM frameworks show unidimensionality. The study established that most of the selected frameworks exposed a high level of reliability. The frequency distribution analysis revealed that the larger part of the constructs had a high mean score and mode. It is concluded that there is a need for a new LSCM framework to fulfill the requirements of the Indian manufacturing industry. Originality/value The present study is focused on checking the suitability of existing LSCM frameworks in the Indian manufacturing sector. It is anticipated that the study will be helpful to the professionals who wish to execute appropriate LSCM framework in the manufacturing organization.
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Khorasani, Sasan T., Jennifer Cross, and Omid Maghazei. "Lean supply chain management in healthcare: a systematic review and meta-study." International Journal of Lean Six Sigma 11, no. 1 (January 9, 2020): 1–34. http://dx.doi.org/10.1108/ijlss-07-2018-0069.
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PurposeBy applying a systematic literature review, this paper aims to identify the major healthcare problem domains (i.e.target areas) for lean supply chain management (LSCM) and to provide a list of the most common techniques for implementing LSCM in healthcare. Moreover, this study intends to investigate various contingency factors that may have influenced the selection of LSCM target areas or the application of LSCM techniques by healthcare organizations.Design/methodology/approachA systematic literature review was carried out following the method presented by Tranfield et al. (2003). Thereby, 280 peer-reviewed journal articles, published between 1995 and 2018, were selected, profiled and reviewed. In total, 75 papers were also selected for a qualitative analysis, known as meta-study, on the basis of high relevancy to the research objectives.FindingsThis work extracts, from previous research, a set of target areas for improving supply chain in healthcare by applying lean approaches. The work also unifies the language of lean thinking and supply chain in healthcare by defining metaphors in circumstances under which healthcare organizations pursue similar objectives from their supply chain management and lean programs (Schmitt, 2005). This paper also outlines a list of applications of lean for supply chain improvement in healthcare. Finally, a set of contingency factors in the field of lean supply chain in healthcare is found via the published literature.Practical implicationsThis paper provides insights for decision-makers in the healthcare industry regarding the benefits of implementing LSCM, and it identifies contingency factors affecting the implementation of LSCM principles for healthcare. Implementing LSCM can help healthcare organizations improve the following domains: internal interaction between employees, supply chain cost management, medication distribution systems, patient safety and instrument utilization.Social implicationsThe research shows potential synthesis of LSCM with the healthcare industry’s objectives, and, thus, the outcome of this research is likely to have positive influence on the quality and cost of healthcare services. The objectives of the healthcare industry are cost reduction and providing better service quality, and LSCM implementation could be an effective solution to help healthcare to achieve these objectives.Originality/valueThe prime value of this paper lies in conducting a systematic literature review using a meta-study to identify the major factors of implementing LSCM in healthcare. Only a few other studies have been published in the literature about LSCM in healthcare.
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Kummer, Sebastian, David M. Herold, Mario Dobrovnik, Jasmin Mikl, and Nicole Schäfer. "A Systematic Review of Blockchain Literature in Logistics and Supply Chain Management: Identifying Research Questions and Future Directions." Future Internet 12, no. 3 (March 23, 2020): 60. http://dx.doi.org/10.3390/fi12030060.
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Potential blockchain applications in logistics and transport (LSCM) have gained increasing attention within both academia and industry. However, as a field in its infancy, blockchain research often lacks theoretical foundations, and it is not clear which and to what extent organizational theories are used to investigate blockchain technology in the field of LSCM. In response, based upon a systematic literature review, this paper: (a) identifies the most relevant organizational theories used in blockchain literature in the context of LSCM; and (b) examines the content of the identified organizational theories to formulate relevant research questions for investigating blockchain technology in LSCM. Our results show that blockchain literature in LSCM is based around six organizational theories, namely: agency theory, information theory, institutional theory, network theory, the resource-based view and transaction cost analysis. We also present how these theories can be used to examine specific blockchain problems by identifying blockchain-specific research questions that are worthy of investigation.
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Nitsche, Benjamin. "Decrypting the Belt and Road Initiative: Barriers and Development Paths for Global Logistics Networks." Sustainability 12, no. 21 (November 2, 2020): 9110. http://dx.doi.org/10.3390/su12219110.
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The Belt and Road Initiative (BRI) is an ambitious infrastructure and development project promoting sustainable economic growth through facilitating prosperous trade across Eurasia and Africa; however, its potential remains underexploited. Logistics networks along BRI routes face several challenges that hinder efficient operations. In addition, although uncertain of how the initiative will develop, logistics and supply chain management (LSCM) practitioners must align their networks with future developments. This study aims to synthesize current barriers to the BRI from an LSCM perspective; propose strategies for dealing with them; and outline and assess conceivable BRI development scenarios to create awareness for possible international logistics network developments. The study builds on a structured and moderated Nominal Group Technique exercise among 15 LSCM professionals to extract current barriers, mitigation strategies, and potential development scenarios, followed by a survey among 52 LSCM professionals to assess those issues. The study synthesizes and assesses 17 BRI barriers for LSCM practice and proposes 20 strategies for dealing with them, assessed in terms of effectiveness and complexity. Moreover, 14 development scenarios are assessed in terms of their probability of becoming reality and impact on the vulnerability of logistics networks, categorized into four scenario clusters (monitor, prepare, propel, and exploit) for guiding LSCM practice.
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Hanson, Hugo H., James E. Reilly, Rebecca Lee, William G. Janssen, and Greg R. Phillips. "Streamlined Embedding of Cell Monolayers on Gridded Glass-Bottom Imaging Dishes for Correlative Light and Electron Microscopy." Microscopy and Microanalysis 16, no. 6 (October 20, 2010): 747–54. http://dx.doi.org/10.1017/s1431927610094092.
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AbstractCorrelative light and electron microscopy (CLEM) has facilitated study of intracellular trafficking. Routine application of CLEM would be advantageous for many laboratories but previously described techniques are particularly demanding, even for those with access to laser scanning confocal microscopy (LSCM) and transmission electron microscopy (TEM). We describe streamlined methods for TEM of green fluorescent protein (GFP)-labeled organelles after imaging by LSCM using gridded glass bottom imaging dishes. GFP-MAP 1A/1B LC3 (GFP-LC3) transfected cells were treated with rapamycin, fixed and imaged by LSCM. Confocal image stacks were acquired enabling full visualization of each GFP-LC3 labeled organelle. After LSCM, cells were embedded for TEM using a simplified two step method that stabilizes the glass bottom such that the block can be separated from the glass by mild heating. All imaging and TEM processing are performed in the same dish. The LSCM imaged cells were relocated on the block and serial sectioned. Correlation of LSCM, DIC, and TEM images was facilitated by cellular landmarks. All GFP labeled structures were successfully reidentified and imaged by serial section TEM. This method could make CLEM more accessible to nonspecialized laboratories with basic electron microscopy expertise and could be used routinely to confirm organelle localization of fluorescent puncta.
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Mahadi, Fazera. "LOGISTICS AND SUPPLY CHAIN MANAGEMENT IN HALAL PHARMACEUTICAL RESEARCH: A SYSTEMATIC LITERATURE REVIEW USING PRISMA." Journal of Halal Science and Technology 2, no. 1 (June 30, 2023): 54–67. http://dx.doi.org/10.59202/jhst.v2i1.668.
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Halal food research has gained significant attention, overshadowing the exploration of halal pharmaceutical research. Nevertheless, the importance of addressing halal status concerns and ensuring the appropriate handling of halal pharmaceutical products is gradually rising. Consequently, the complexities of logistics, supply chain management, and transparency in the context of halal pharmaceuticals have become pertinent issues. Surprisingly, the existing literature on halal-related topics remains a 'grey area' when it comes to LSCM (LSCM) in the pharmaceutical sector. To address this gap, this paper aims to investigate the LSCM aspects of halal pharmaceutical research. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology was employed to systematically screen, review, and synthesise the literature on halal pharmaceutical research. Through this review, we aim to examine the current LSCM trends and the state of research in the field of halal pharmaceuticals. Despite some notable advancements, halal pharmaceutical research remains largely understudied. Moreover, there are significant gaps in the existing literature, particularly from an LSCM perspective, which hinders knowledge expansion and practical applications. By conducting a systematic review from the LSCM lens, this study aims to contribute valuable conceptual insights and practical implications that will serve as a foundation for future research in the field of halal pharmaceuticals.
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Yue, Xiangling, Ana Arenillas, and John T. S. Irvine. "Application of infiltrated LSCM–GDC oxide anode in direct carbon/coal fuel cells." Faraday Discussions 190 (2016): 269–89. http://dx.doi.org/10.1039/c6fd00001k.
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Hybrid direct carbon/coal fuel cells (HDCFCs) utilise an anode based upon a molten carbonate salt with an oxide conducting solid electrolyte for direct carbon/coal conversion. They can be fuelled by a wide range of carbon sources, and offer higher potential chemical to electrical energy conversion efficiency and have the potential to decrease CO2 emissions compared to coal-fired power plants. In this study, the application of (La, Sr)(Cr, Mn)O3 (LSCM) and (Gd, Ce)O2 (GDC) oxide anodes was explored in a HDCFC system running with two different carbon fuels, an organic xerogel and a raw bituminous coal. The electrochemical performance of the HDCFC based on a 1–2 mm thick 8 mol% yttria stabilised zirconia (YSZ) electrolyte and the GDC–LSCM anode fabricated by wet impregnation procedures was characterized and discussed. The infiltrated oxide anode showed a significantly higher performance than the conventional Ni–YSZ anode, without suffering from impurity formation under HDCFC operation conditions. Total polarisation resistance (Rp) reached 0.8–0.9 Ω cm2 from DCFC with an oxide anode on xerogel and bituminous coal at 750 °C, with open circuit voltage (OCV) values in the range 1.1–1.2 V on both carbon forms. These indicated the potential application of LSCM–GDC oxide anode in HDCFCs. The chemical compatibility of LSCM/GDC with carbon/carbonate investigation revealed the emergence of an A2BO4 type oxide in place of an ABO3 perovskite structure in the LSCM in a reducing environment, due to Li attack as a result of intimate contact between the LSCM and Li2CO3, with GDC being stable under identical conditions. Such reaction between LSCM and Li2CO3 was not observed on a LSCM–YSZ pellet treated with Li–K carbonate in 5% H2/Ar at 700 °C, nor on a GDC–LSCM anode after HDCFC operation. The HDCFC durability tests of GDC–LSCM oxide on a xerogel and on raw bituminous coal were performed under potentiostatic operation at 0.7 V at 750 °C. The degradation mechanisms were addressed, especially on raw coal.
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Zheng, Jiming, Zheng Luo, and Zhirui Tang. "An Image Encryption Algorithm Based on Multichaotic System and DNA Coding." Discrete Dynamics in Nature and Society 2020 (September 27, 2020): 1–16. http://dx.doi.org/10.1155/2020/5982743.
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In this paper, an improved two-dimensional logistic-sine coupling map (N2D-LSCM) and an improved Henon map (NHenon) are proposed. Furthermore, by combining N2D-LSCM and NHenon map, an image encryption algorithm is proposed based on these two chaotic systems and DNA coding. The chaotic sequences generated by N2D-LSCM are used as the parameters of NHenon. In the scrambling stage, DNA encoding is carried out for pixels after scrambling by two chaotic sequences generated by N2D-LSCM; in the stage of diffusion, DNA random coding acts on random matrix obtained by two chaotic sequences generated by NHenon, and DNA XOR operation is carried out with the image obtained in the scrambling stage to diffuse. Compared with other 2D map for image encryption algorithm, this algorithm exhibits good security and holds high efficiency.
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Pardi, Hamizah, Mohd Nizam Ab Rahman, Nizaroyani Saibani, and Ariff Azly Muhamed. "A Systematic Literature Review of Critical Success Factors and Barriers in Lean Supply Chain Management." Jurnal Kejuruteraan 36, no. 2 (March 30, 2024): 533–58. http://dx.doi.org/10.17576/jkukm-2024-36(2)-15.
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The objective of this study is to conduct a systematic literature review to identify the barriers and critical success factor that influence in implementation of Lean Supply Chain Management (LSCM) practices in industry. Through this systematic literature review (SLR), it is expected to identify the main gaps related to LSCM implementation in industry and discuss the relevance of research in this topic for indicating the future research direction. A SLR was developed and determined, which involved the selecting of keyword, developing of research question, searching and evaluation of literature for 53 research articles. The content of present of literature was reviewed, critically analysed and synthesised from the perspective of barrier and critical success factors that influence the implementation of LSCM. This study reveals that relationship with supplier, commitment and support by top management are the key critical success factor in lean implementation. Furthermore, the results showed that lack of management commitment and leadership, lack of training and skills, lack of system view or strategy are the barriers in lean implementation. Based on the extensive systematic review of literature, we found of the consistency data between Barriers and Critical Success Factor. This study extends previous studies on LSCM, strengthens the body of knowledge on the issue and consolidates the key LSCM activities, barriers and critical success factors associated with its implementation. The clear identification of these topics can allow researchers and practitioners to predict occasional problems and to set the right standards for the implementation of LSCM.
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Mastropasqua, Leonardo, Luca Agnifili, Rodolfo Mastropasqua, Vincenzo Fasanella, Mario Nubile, Lisa Toto, Paolo Carpineto, and Marco Ciancaglini. "In Vivo Laser Scanning Confocal Microscopy of the Ocular Surface in Glaucoma." Microscopy and Microanalysis 20, no. 3 (February 27, 2014): 879–94. http://dx.doi.org/10.1017/s1431927614000324.
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AbstractOver the past decade, knowledge about the ocular surface in glaucoma has significantly increased through the use of in vivo laser scanning confocal microscopy (LSCM). This in vivo imaging method can show modifications at the cellular level induced by anti-glaucoma drugs on ocular surface structures and adnexa in the eye. High-quality images of the conjunctiva, cornea, limbus, meibomian glands, and lymphoid structures during therapy can be obtained. In addition, LSCM opened new fields of research on the patho-physiology of aqueous humor (AH) hydrodynamics in untreated, and in medically or surgically treated glaucomatous patients. In these conditions, an enhancement of the trans-scleral AH outflow contributed to clarification of the mechanism of action of different anti-glaucoma medications and surgical approaches. Finally, the use of LSCM represented a huge advance in evaluation of bleb functionality after filtration surgery, defining the hallmarks of AH filtration through the bleb-wall and distinguishing functional from nonfunctional blebs. Thus, signs seen with LSCM may anticipate clinical failure, guiding the clinician in planning the appropriate timing of the various steps in bleb management. In this review we summarize the current knowledge about in vivo LSCM of the ocular surface in glaucoma.
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Li, Jian Guo, and Ying Li. "Characterizing the Influence of Drying on Ink Absorption Using Reconstructed Images by Laser Scanning Confocal Microscopy." Advanced Materials Research 904 (March 2014): 59–62. http://dx.doi.org/10.4028/www.scientific.net/amr.904.59.
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The objective of this experiment was to investigate the relationship between drying and ink absorption using laser scanning confocal microscopy (LSCM). Fluorescent ink was used to observe and characterize ink penetration and distribution by LSCM. Three-dimensional images of ink penetration were obtained by reconstructing all XY plane images. Reconstructed images were used to describe ink absorption in coated paper by LSCM. The results implied that it was reliable and effective using LSCM to characterize the ink penetration depth and distribution uniformity. This method could not damage the specimen and did not need fluorescent dye to stain the specimen, which decreased the errors by hand operation. The results indicated that drying temperature affected ink penetration depth and distribution evenness. Higher and lower drying temperature could not contribute to ink absorption uniformity. With the drying temperature increasing, ink penetration depth in coated paper increased.
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Michálek, Jan, Karel Štěpka, Michal Kozubek, Jarmila Navrátilová, Barbora Pavlatovská, Markéta Machálková, Jan Preisler, and Adam Pruška. "Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids." Microscopy and Microanalysis 25, no. 6 (October 1, 2019): 1311–22. http://dx.doi.org/10.1017/s1431927619014983.
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AbstractSpheroids—three-dimensional aggregates of cells grown from a cancer cell line—represent a model of living tissue for chemotherapy investigation. Distribution of chemotherapeutics in spheroid sections was determined using the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Proliferating or apoptotic cells were immunohistochemically labeled and visualized by laser scanning confocal fluorescence microscopy (LSCM). Drug efficacy was evaluated by comparing coregistered MALDI MSI and LSCM data of drug-treated spheroids with LSCM only data of untreated control spheroids. We developed a fiducial-based workflow for coregistration of low-resolution MALDI MS with high-resolution LSCM images. To allow comparison of drug and cell distribution between the drug-treated and untreated spheroids of different shapes or diameters, we introduced a common diffusion-related coordinate, the distance from the spheroid boundary. In a procedure referred to as “peeling”, we correlated average drug distribution at a certain distance with the average reduction in the affected cells between the untreated and the treated spheroids. This novel approach makes it possible to differentiate between peripheral cells that died due to therapy and the innermost cells which died naturally. Two novel algorithms—for MALDI MS image denoising and for weighting of MALDI MSI and LSCM data by the presence of cell nuclei—are also presented.
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Terasaki, Hidenori, and Yu Ichi Komizo. "In Situ Study of Microstructural Development during Phase Transformation in Welding Process." Solid State Phenomena 172-174 (June 2011): 1261–66. http://dx.doi.org/10.4028/www.scientific.net/ssp.172-174.1261.
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The applications of laser scanning confocal microscopy (LSCM) system were introduced to the track the phase transformation of steel. For the low and high carbon steel, the microstructural change along various thermal cycles was directly observed with LSCM system. The nature of non-themoelastic martensite was directly observed. Furthermore, direct checking of the nucleation site of ferrite phase was carried out for aluminum killed weld metal and titanium killed-steel. It was carried out concerning to acicular ferrite formation in high strength and low alloy steel. The assessment of the pinning effect of alumiunu nitride on the austenite phase at high temperature was also carried out by using LSCM system.
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López-Cepero, José M., J. J. Quispe Cancapa, António Ramirez de Arellano-López, and Julian Martínez-Fernández. "Fractographic Studies of Sapphire Fibers Using Laser Scanning Confocal Microscopy." Key Engineering Materials 290 (July 2005): 280–83. http://dx.doi.org/10.4028/www.scientific.net/kem.290.280.
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Laser scanning confocal microscopy (LSCM) is a microscopic technique which allows for height discrimination. The ability to gather 3D data, along with adequate resolution (around 400 nm), makes the technique suitable for fractography; however, its applications in this area are not sufficiently explored. In this work, LSCM and SEM are applied to the study of fracture surfaces in sapphire and ruby fibers submitted to tensile stress in high-temperature conditions. The obtained qualitative and quantitative information demonstrates the validity of LSCM as a fractographical technique, allowing for clear identification of fractographical features and providing novel insight in the phenomenon of subcritical crack growth (SCG).
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Gerlach, Benno, Simon Zarnitz, Benjamin Nitsche, and Frank Straube. "Digital Supply Chain Twins—Conceptual Clarification, Use Cases and Benefits." Logistics 5, no. 4 (December 6, 2021): 86. http://dx.doi.org/10.3390/logistics5040086.
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Background: Digital supply chain twins (DSCT) are gaining increased attention in academia and practice as they emerge as one of the most important trends in logistics and supply chain management (LSCM). Still, there seems to be no common understanding of the term in scientific literature. Moreover, the broad field of LSCM allows for a multitude of feasible application areas and use cases, yet there exists no conclusive list of them as to date. Methods: This study builds upon a systematic literature review of 66 DSCT articles to identify application areas of DSCT in LSCM as well as specific use cases and their respective intended benefits. Results: To start with, the study derives a unified definition of DSCTs, including possible scopes of applications. Afterwards, five application areas of DSCT in LSCM are synthesized as well as 14 individual use cases and their respective intended benefits. Conclusions: The study leads towards a conceptual clarification of DSCT that is of importance for research and practice alike. For managers it additionally provides up-to-date use cases to guide DSCT applications in practice.
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Takeda-Berger, Satie Ledoux, Guilherme Luz Tortorella, Carlos Manuel Taboada Rodriguez, Enzo Morosini Frazzon, Tamie Takeda Yokoyama, and Marco Aurélio de Oliveira. "Analysis of the relationship between barriers and practices in the lean supply chain management." International Journal of Lean Six Sigma 12, no. 3 (January 13, 2021): 607–26. http://dx.doi.org/10.1108/ijlss-01-2019-0003.
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Purpose The purpose of this paper is to classify the main barriers related to the implementation of lean supply chain management (LSCM) and prioritize its main practices. A case study approach was conducted to obtain values for the intensity of the relationship between LSCM practices and barriers to mitigate or even anticipate difficulties in its implementation. Design/methodology/approach The case study in a company in southern Brazil that is in lean implementation is used for conducting this research. The methodology was structured in three steps, namely, classification of the main barriers through risk analysis, selection of LSCM practices appropriate to the company context and ranking and prioritization of the main practices of LSCM related to the barriers to lean implementation. Findings As a result, with the combination of two approaches, failure mode and effect analysis (FMEA) and analytical hierarchical process (AHP), it was possible to obtain values for the intensity of the relationship between LSCM practices and barriers. From the 12 barriers, 5 reached a high-risk degree that can be mitigated, and among the 18 practices found, 7 have the potential to be implemented. Originality/value This research allows direct efforts to continuously improve the supply chain to mitigate or even anticipate difficulties in its implementation. The proposed methodology, combining FMEA and AHP is easy to apply and understand, allowing managers and professionals to replicate it in the context of their supply chain and verify results similar and comparable to those obtained here.
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Marinova, Teodora L., Vesela Ivancheva, Svilena S. Peeva, and Christina N. Grupcheva. "Comparison of Four Methods for Corneal Thickness Measurement." Journal of Biomedical and Clinical Research 6, no. 1 (September 1, 2013): 37–42. http://dx.doi.org/10.1515/jbcr-2015-0101.
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Summary The aim of the study was to evaluate the thickness of the normal cornea in order to establish correlation between four methods of measuring including: ultrasound pachymetry (USP), anterior segment optical coherence tomography (ASOCT), non-contact tono/pachymetry (TONOPACHY) and laser-scanning confocal microscopy (LSCM). The study was based on evaluating repeatability and comparability of four different methods formeasuring the corneal thickness. Non contact specular microscopy was first performed on all 27 patients (aged between 20 and 24 years) to evaluate corneal characteristics and confirm the absence of pathological changes. Each participant was examined by USP, ASOCT and TONOPACHY, and 13 eyes of 10 persons were also examined by LSCM. The values of average central corneal thickness measured by USP, ASOCT, TONOPACHY and LSCM were 532.20±4.5 pm, 553.33±12.1 pm, were 548.20±5.62 pm and 573.33±7.22 pm, respectively. There was a high correlation between the instruments. The mean differences for central corneal thickness measurements were 41.43±1.67 pm between USP and LSCM, 20.43±2.4 pm between USP and ASOCT, and 22.1 ±3.88 pm between USB and TONOPACHY. Anterior segment optical coherence tomography overestimated corneal thickness as compared with that measured by USP, which is believed to be a gold standard. Anterior segment optical coherence tomography had better agreement with USP, as compared with LSCM. However, the results of measured cornea thickness by TONOPACHY were very close to cornea thickness measured by ASOCT.
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Li, Ying, and Bei Hai He. "Investigation into the Effect of Coating Thickness on Ink Penetration by LSCM." Advanced Materials Research 174 (December 2010): 350–53. http://dx.doi.org/10.4028/www.scientific.net/amr.174.350.
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Cutting is a relative accurate method in the literatures about ink penetration measurement at present. But cutting may bring some damages to paper, which reduces reliability of measurement result. The objective of this experiment was to investigate the influence of coating thickness on ink penetration and characterize the penetration depth through quantitative analysis by Laser Scanning Confocal Microscope (LSCM). Fluorescent ink was used to observe and characterize ink penetration by LSCM. Three-dimensional images of ink pigment penetration were obtained by reconstructing all XY plane images. The results implied that it was reliable and effective method using LSCM to characterize the ink penetration depth and distribution uniformity. It could be concluded that the print quality could be adjusted and improved by increasing the coating thickness.
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Li, Hongqiao, and Matthias S. Ullrich. "Characterization and Mutational Analysis of Three Allelic lsc Genes Encoding Levansucrase inPseudomonas syringae." Journal of Bacteriology 183, no. 11 (June 1, 2001): 3282–92. http://dx.doi.org/10.1128/jb.183.11.3282-3292.2001.
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ABSTRACT In the plant pathogen Pseudomonas syringaepv. glycinea PG4180 and other bacterial species, synthesis of the exopolysaccharide levan is catalyzed by the extracellular enzyme levansucrase. The results of Southern blotting and PCR analysis indicated the presence of three levansucrase-encoding genes in strain PG4180: lscA, lscB, andlscC. In this study, lscB andlscC were cloned from a genomic library of strain PG4180. Sequence analysis of the two lsc genes showed that they were virtually identical to each other and highly similar to the previously characterized lscA gene.lscA and lscC had a chromosomal location, whereas lscB resided on an indigenous plasmid of PG4180. Mutants with impaired expression of individual lsc genes and double mutants were generated by marker exchange mutagenesis. Determination of levansucrase activities in these mutants revealed that the lscB gene product was secreted but not that oflscA or lscC. Our results indicated thatlscB and lscC but not lscAcontributed to periplasmic levan synthesis of PG4180. The lscB lscC double mutant was completely defective in levan formation and could be complemented by either lscB orlscC. Our data suggested a compartment-specific localization of two lsc gene products, with LscB being the secreted, extracellular enzyme and LscC being the predominantly periplasmic levansucrase. Results of Western blot analyses indicated that lscA was not expressed and that lscAwas not associated with levansucrase activities in any particular protein fraction. LscA could be detected in PG4180 only when transcribed from the vector-borne P lac promoter. PCR screening in various P. syringae strains with primers derived from the three characterized lscgenes demonstrated the presence of multiple Lsc isoenzymes in otherP. syringae pathovars.
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Svanberg, Martin. "Guidelines for establishing practical relevance in logistics and supply chain management research." International Journal of Physical Distribution & Logistics Management 50, no. 2 (March 2, 2020): 215–32. http://dx.doi.org/10.1108/ijpdlm-11-2018-0373.
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PurposeRigor and practical relevance are the foundations for logistics and supply chain management (LSCM) as an applied discipline. Whereas there are well-founded criteria for establishing methodological rigor, researchers must provide their own credible logic as to why their papers can influence practice. Accordingly, this paper aims to develop guidelines for establishing practical relevance in research papers.Design/methodology/approachA literature review of LSCM, marketing, operations management and management journals forms the foundation for these guidelines.FindingsRelevance criteria are identified; research should be problem-driven, timely and important, and findings should be implementable, nonobvious, novel and not too costly. Measures for researchers demonstrating the fulfillment of these criteria are identified as practitioner input, gray literature, funding, practitioner involvement and feedback. Researchers should also clearly articulate both problem relevance and the relevance of their findings.Research limitations/implicationsA lack of practical relevance is among the reasons for the rejection of papers by LSCM journals, but researchers can overcome this obstacle using these guidelines.Practical implicationsAt a metalevel, this paper contributes to research with greater practical relevance.Originality/valuePractical relevance is emphasized in the editorials of LSCM journals but has not yet been fully conceptualized from the authors' perspective.
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Arkhipov, Arsenii Y., Eduard F. Khaziev, Andrey I. Skorinkin, Ellya A. Bukharaeva, and Dmitry V. Samigullin. "Enhancement of the Temporal Resolution of Fluorescent Signals Acquired by the Confocal Microscope." Microscopy and Microanalysis 26, no. 2 (March 2, 2020): 204–10. http://dx.doi.org/10.1017/s1431927620000148.
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AbstractHere, we describe a method of acquisition of fast fluorescent signals with the help of the laser scanning confocal microscope (LSCM). Our method permits an increase in the temporal resolution of acquired signals. The method is based on LSCM recordings of fast fluorescent signals with the shortest achievable time sweep, which are performed with the help of a proprietary algorithm. A series of recordings is made in multiple steps; at each step, the fluorescent signal is incremented by a time interval smaller than the time sweep of the frame of LSCM. The size of the increment determines the achievable time resolution. The convolution of the recorded images results in a signal with the temporal resolution determined by the chosen time increment. This method was applied to register the change in fluorescence (calcium transient) of calcium dye preloaded into peripheral nerve endings by electrical stimulation of the motor nerve. Calculated parameters of the calcium transient were identical to the parameters obtained earlier with the help of a high-speed camera and photodiode. We conclude that the method described here can be applied for the registration of fast fluorescent signals by LSCM with a high spatial and temporal resolution.
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Erokhina, Maria V., Larisa N. Lepekha, Elena E. Voronezhskaya, Leonid P. Nezlin, Vadim G. Avdienko, and Atadzhan E. Ergeshov. "Application of Laser Scanning Confocal Microscopy for the Visualization of M. tuberculosis in Lung Tissue Samples with Weak Ziehl–Neelsen Staining." Journal of Clinical Medicine 8, no. 8 (August 7, 2019): 1185. http://dx.doi.org/10.3390/jcm8081185.
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One of the key requirements for the diagnosis of pulmonary tuberculosis is the identification of M. tuberculosis in tissue. In this paper, we present the advantages of specific fluorescent antibody labelling, combined with laser scanning confocal microscopy (LSCM), for the detection of M. tuberculosis in histological specimens of lung tissues. We demonstrate that the application of LSCM allows: (i) The automatic acquisition of images of the whole slice and, hence, the determination of regions for subsequent analysis; (ii) the acquisition of images of thick (20–40 μm) slices at high resolution; (iii) single bacteria identification; and (iv) 3D reconstruction, in order to obtain additional information about the distribution, size, and morphology of solitary M. tuberculosis; as well as their aggregates and colonies, in various regions of tuberculosis inflammation. LSCM allows for the discrimination of the non-specific fluorescence of bacteria-like particles and their aggregates presented in histological lung samples, from the specific fluorescence of labelled M. tuberculosis, using spectrum emission analysis. The applied method was effective in the identification of M. tuberculosis in lung histological samples with weak Ziehl–Neelsen staining. Altogether, combining immunofluorescent labelling with the application of LSCM visualization significantly increases the effectiveness of M. tuberculosis detection.
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Podhorská, Bohumila, Miroslav Vetrík, Eva Chylíková-Krumbholcová, Lucie Kománková, Niloufar Rashedi Banafshehvaragh, Miroslav Šlouf, Miroslava Dušková-Smrčková, and Olga Janoušková. "Revealing the True Morphological Structure of Macroporous Soft Hydrogels for Tissue Engineering." Applied Sciences 10, no. 19 (September 24, 2020): 6672. http://dx.doi.org/10.3390/app10196672.
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(1) Background: Macroporous hydrogel scaffolds based on poly [N-(2-hydroxypropyl) methacrylamide] are one of the widely studied biocompatible materials for tissue reparation and regeneration. This study investigated the morphological changes during hydrogel characterization which can significantly influence their future application. (2) Methods: Three types of macroporous soft hydrogels differing in pore size were prepared. The macroporosity was achieved by the addition of sacrificial template particles of sodium chloride of various sizes (0–30, 30–50, and 50–90 µm) to the polymerizing mixture. The 3D structure of the hydrogels was then investigated by scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). The SEM was performed with specimens rapidly frozen to various temperatures, while non-frozen gels were visualized with LSCM. (3 and 4) Results and Conclusion: In comparison to LSCM, the SEM images revealed a significant alteration in the mean pore size and appearance of newly formed multiple connections between the pores, depending on the freezing conditions. Additionally, after freezing for SEM, the gel matrix between the pores and the fine pores collapsed. LSCM visualization aided the understanding of the dynamics of pore generation using sodium chloride, providing the direct observation of hydrogel scaffolds with the growing cells. Moreover, the reconstructed confocal z-stacks were a promising tool to quantify the swollen hydrogel volume reconstruction which is not possible with SEM.
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Wong, Chee Yew. "Celebrating IJPDLM's 50th anniversary: a reflection on its contributions and future directions." International Journal of Physical Distribution & Logistics Management 51, no. 10 (November 16, 2021): 1049–64. http://dx.doi.org/10.1108/ijpdlm-10-2021-0427.
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PurposeThis article celebrates the 50th anniversary of IJPDLM, reflects on the contribution of IJPDLM to the field of logistics and supply chain management (LSCM) and discusses future directions for the journal.Design/methodology/approachDescriptive analysis of manuscripts received and accepted by IJPDLM during 2015–2019 is used to provide an overview of the journal. Content analysis of selected articles is used to highlight important contributions of the journal. Changes made since 2020 are highlighted to inform future directions of IJPDLM. Invited articles are discussed and used to clarify future directions.FindingsIJPDLM has made tremendous progress in informing and shaping the field of LSCM. Key issues addressed include sustainability and reverse logistics, omni-channel, e-commerce, retail logistics, risk, resilience, volatility, and complexity and digital technology innovation. The journal has expanded the use of methods beyond the typical qualitative and quantitative methods to explore the use of design science, experiment, conjoint analysis, qualitative comparative analysis, narrative analysis. The invited articles provide (1) a historical reflection of the purpose of the journal when it was launched, (2) new guidance on how to develop theories using literature review and grounded theories and (3) understanding of startups and supply chain ecosystems.Practical implicationsSome exemplar articles are highlighted to explain how IJPDLM informs LSCM managers, companies and policy makers.Originality/valueThis article explains the recent development and sets future directions for the LSCM field.
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Li, Ying, and Bei Hai He. "Study on the Effect of Drying on Binder Z- Directional Distribution in Coated Paper." Advanced Materials Research 233-235 (May 2011): 1608–13. http://dx.doi.org/10.4028/www.scientific.net/amr.233-235.1608.
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The main objective of this study was to investigate the effect of drying condition on the binder distribution and characterize the binder migration in the method of numerical and visual analysis by Scanning Electrical Microscopy (SEM), Atomic Force Microscopy (AFM), and Laser Scanning Confocal Microscope (LSCM). In this study, Rhodamine B was used to stain the binder and as the probe to characterize z-directional distribution of the binder using LSCM. The results implied that it was reliable and effective method using LSCM to characterize the z- directional distribution of binder and analyze the influence of dry condition on binder migration. It could be concluded that the dry temperature played an important effect on binder distribution in coated paper and high dry temperature accelerated binder migration to the coating surface, however low drying temperature made binder migrate towards the interior of base paper. Moderate drying is good to binder distribution and coating layer forming.
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McCoy, Karen L., Andrew G. Dillin, and Ardythe A. McCracken. "Visualizing the intracellular membrane system of yeast cells using a laser scanning confocal microscope." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 274–75. http://dx.doi.org/10.1017/s0424820100147211.
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Progress has been made in developing new preparative techniques for electron microscopic visualization of the intracellular structures of yeast. In addition, development of the laser scanning confocal microscope (LSCM) has provided improved resolution for fluorescent microscopy. We asked whether the LSCM in combination with new preparative techniques could be used for comparable investigative research of the intracellular organizaton of the yeast cell.To investigate this possibility, a BioRad MRC600 LSCM equipped with a krypton/argon laser and integrated computer imaging capabilities, was used to study various dipliod strains of the yeast Saccharomyces cerevisiae. Cells were treated with the lipophilic, cationic fluorescent dye DiOC6 (3,3’-dihexyloxacarbocyanine iodide), which has been used to visualize intracellular membrane structures, and in particular the endoplasmic reticulum of mammalian cells and living yeast cells. Since one of our interests is the intracellular localization of proteins in the yeast cell, we utilized transformed yeast cells expressing a human gene encoding a protein that inappropriately accumulates in the endoplasmic reticulum (ER).
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Lee, Sang Won, Minkyu KIm, Seok Hee Lee, and Tae Ho Shin. "Enhancing the Catalytic Activity of Ce(Mn, Fe)O2 on a Perovskite-Based Electrode via Spraying for High-Temperature CO2 Electrolysis." ECS Transactions 111, no. 6 (May 19, 2023): 1153–58. http://dx.doi.org/10.1149/11106.1153ecst.
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Solid oxide electrolysis cells (SOECs) have the potential to be highly efficient devices for high-temperature CO2electrolysis. However, commonly used Ni-based cathodes suffer from redox instability under CO/CO2 conditions. La(Sr)Cr(Mn)O3 (LSCM) is a promising alternative due to its coking resistance, but it has poor catalytic activity on CO2reduction. To address this, Ce(Mn, Fe)O2 (CMF) was added as a catalyst to accelerate CO2 adsorption. In this study, the non-touchable coating process by spraying was employed to fabricate the well-decorated embedded CMF on LSCM surface, without complicated pretreatment and infiltration. The performances of the highly decorated electrode surface with CMF nano catalyst were measured at 1123 K, exhibiting a maximum power density of 0.503 W cm-2 in fuel cell mode (H2) and electrolysis performance of 0.835 A cm-2 at 1.5 V in CO2 reduction. This approach shows potential for enhancing the catalytic activity of LSCM cathodes and advancing the development of SOECs for sustainable energy production.
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Xing, Jie, Xiu Hua Chen, Wen Hui Ma, Rui Li, Jian Jun Yang, and Jie Yu. "Anode-Supported LSGM Films Prepared by Slurry Spin Coating." Advanced Materials Research 898 (February 2014): 123–27. http://dx.doi.org/10.4028/www.scientific.net/amr.898.123.
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La0.9Sr0.1Ga0.8Mg0.2O2.85 (LSGM) electrolyte films were successfully prepared by slurry spin coating method on porous La0.7Sr0.3Cr0.5Mn0.5O2.75 (LSCM) anode substrates. Ethyl celluloses content, coating cycles for slurry spin coating on the fabrication LSGM electrolyte films were investigated. The compatibility between LSGM and LSCM powders, microstructures and electricity conductivity of fabricated LSGM films were examined using XRD, SEM and electrochemical workstation. The film with good apparent morphology and electrical conductivity were obtained when the operating parameters were setted as the content of ethyl cellulose 10wt%, and the coating cycles 5.
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XIAO Yun, 肖昀, 张运海 ZHANG Yun-hai, 王真 WANG Zhen, and 黎发志 LI Fa-zhi. "Effect of incident laser on resolution of LSCM." Optics and Precision Engineering 22, no. 1 (2014): 31–38. http://dx.doi.org/10.3788/ope.20142201.0031.
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Clark Brelje, T., and Robert L. Sorenson. "Multi-color laser scanning confocal microscopy with a krypton/argon ion laser." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 406–7. http://dx.doi.org/10.1017/s0424820100086337.
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Fluorescence is presently the most important imaging mode in biological confocal microscopy. The optical properties of laser scanning confocal microscopy (LSCM) are particularly favorable for fluorescence microscopy since the generally high signal-to-background ratio is enhanced by LSCM by rejecting out-of-focus fluorescent emissions. In addition, this improved imaging capability along the optical (z-)axis allows the optical sectioning of specimens by adjusting the plane of focus. This removes one of the most severe limitations of convential fluorescence microscopy, the necessity to examine monolayers of cells or thin sections of tissues.However, the advantages of LSCM for multi-color fluorscence microscopy are critically dependent on the availability of suitable light sources and fluorophores. By far the most commonly used light source is a small, air-cooled argon ion laser with emission wavelengths at 488 and 514 nm. Although the most frequently used fluorophores, fluorescein (FITC) and tetramethylrhodamine, can be excited by these wavelengths, it is impossible to specifically excite each fluorophore in the presence of the other fluorophore.
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Amemiya, Kei, Xiankun Zeng, Jeremy J. Bearss, Christopher K. Cote, Carl Soffler, Robert C. Bernhards, Jennifer L. Dankmeyer, et al. "Laser Scanning Confocal Microscopy Was Used to Validate the Presence of Burkholderia pseudomallei or B. mallei in Formalin-Fixed Paraffin Embedded Tissues." Tropical Medicine and Infectious Disease 5, no. 2 (April 29, 2020): 65. http://dx.doi.org/10.3390/tropicalmed5020065.
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Burkholderia pseudomallei and B. mallei are Gram-negative, facultative intracellular bacteria that cause melioidosis and glanders, respectively. Currently, there are no vaccines for these two diseases. Animal models have been developed to evaluate vaccines and therapeutics. Tissues from infected animals, however, must be fixed in formalin and embedded in paraffin (FFPE) before analysis. A brownish staining material in infected tissues that represents the exopolysaccharide of the pathogen was seen by bright field microscopy but not the actual microorganism. Because of these results, FFPE tissue was examined by laser scanning confocal microscopy (LSCM) in an attempt to see the microorganism. Archival FFPE tissues were examined from ten mice, and five nonhuman primates after exposure to B. pseudomallei or B. mallei by LSCM. Additionally, a historical spleen biopsy from a human suspected of exposure to B. mallei was examined. B. pseudomallei was seen in many of the infected tissues from mice. Four out of five nonhuman primates were positive for the pathogen. In the human sample, B. mallei was seen in pyogranulomas in the spleen biopsy. Thus, the presence of the pathogen was validated by LSCM in murine, nonhuman primate, and human FFPE tissues.
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Woolf, N. K., D. V. Jaquish, F. J. Koehrn, V. L. Woods, and D. A. Peterson. "Improved resolution of fibronectin mRNA expression in the inner ear using laser scanning confocal microscopy." Journal of Histochemistry & Cytochemistry 44, no. 1 (January 1996): 27–34. http://dx.doi.org/10.1177/44.1.8543778.
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We describe a modified in situ hybridization protocol for localizing and quantifying fibronectin gene expression at the cellular level in paraffin sections of rat temporal bone. When combined with a novel analytical approach using laser scanning confocal microscopy (LSCM), this protocol significantly improved the resolution, sensitivity, and specificity of existing procedures for evaluating fibronectin synthesis in developing inner ear. For simultaneous viewing of cochlear anatomy and the autoradiographic signal, transmitted light images of the cochlea were collected separately from LSCM reflected light images of the autoradiographic silver grains and then the two images were electronically merged. Within the first 2 microns below the surface of the emulsion, silver grains were clustered specifically over hybridized cells. In contrast, nonspecific silver grain development (i.e., background noise) was confined primarily to the lower 5 microns of the emulsion adjacent to the tissue section. Limiting the volume of the emulsion examined in the LSCM analysis, i.e., restricting the range of optical sectioning to the first 2 micron below the surface of the emulsion, effectively minimized nonspecific background noise and maximized the specificity of the hybridization signal. The improvements offered by the described methodological approaches are equally appropriate for non-calcified tissues.
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Li, Ying, Wen Juan Gu, and Bang Gui He. "Numerical Characterization of the Effect of Coating Solid on Binder Distribution." Applied Mechanics and Materials 496-500 (January 2014): 388–91. http://dx.doi.org/10.4028/www.scientific.net/amm.496-500.388.
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Binder is the essential component of coating color recipe. The amount and type of latex binder has a significant effect on both the structure and chemical-physical properties of coated paper, which affect critical surface properties, e.g., print gloss, roughness, ink setting rate via liquid absorption, and print mottle The main aim of this study was to investigate the effect of coating solid on the binder distribution in the method of numerical analysis by Laser Scanning Confocal Microscope (LSCM). In this study, Rhodamine B was used to stain the binder and as the probe to characterize z-directional distribution of the binder using LSCM. The results implied that it was reliable and effective method using LSCM to characterize the z-directional distribution of binder and analyze the influence of coating solid on binder distribution in the z-direction. It could be concluded that the coating solid played an important effect on binder distribution in coated paper and low coating solid with more water accelerated binder migration to the interior of base paper, sample with 60% coating solid is good to binder distribution and coating layer forming.
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Fasanella, Vincenzo, Luca Agnifili, Rodolfo Mastropasqua, Lorenza Brescia, Federico Di Staso, Marco Ciancaglini, and Leonardo Mastropasqua. "In VivoLaser Scanning Confocal Microscopy of Human Meibomian Glands in Aging and Ocular Surface Diseases." BioMed Research International 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/7432131.
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Meibomian glands (MGs) play a crucial role in the ocular surface homeostasis by providing lipids to the superficial tear film. Their dysfunction destabilizes the tear film leading to a progressive loss of the ocular surface equilibrium and increasing the risk for dry eye. In fact, nowadays, the meibomian gland dysfunction is one of the leading causes of dry eye. Over the past decades, MGs have been mainly studied by using meibography, which, however, cannot image the glandular structure at a cellular level. The diffusion of thein vivolaser scanning confocal microscopy (LSCM) provided a new approach for the structural assessment of MGs permitting a major step in the noninvasive evaluation of these structures. LSCM is capable of showing MGs modifications during aging and in the most diffuse ocular surface diseases such as dry eye, allergy, and autoimmune conditions and in the drug-induced ocular surface disease. On the other hand, LSCM may help clinicians in monitoring the tissue response to therapy. In this review, we summarized the current knowledge about the role ofin vivoLSCM in the assessment of MGs during aging and in the most diffuse ocular surface diseases.
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Shields, Lisa B. E., Ian S. Mutchnick, Dennis S. Peppas, and Eran Rosenberg. "Importance of Physical Examination and Imaging in the Detection of Tethered Cord Syndrome." Global Pediatric Health 6 (January 2019): 2333794X1985141. http://dx.doi.org/10.1177/2333794x19851419.
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Tethered cord syndrome (TCS) is a type of occult spinal dysraphism that may lead to permanent neurologic and orthopedic deficits. Infants with TCS may have lumbosacral cutaneous malformations (LsCMs). We studied 67 infants referred to a single pediatric urology practice for a urological concern unrelated to occult spinal dysraphism with no prior diagnosis of LsCM between March 1, 2015 and September 30, 2018. Each infant underwent a spinal ultrasound. If an abnormality was detected, they were referred to a pediatric neurosurgeon. The most common cutaneous manifestations were duplicated or bifurcated (46%) gluteal folds and gluteal asymmetry (16%). Fourteen (21%) of the 67 patients had an abnormal spinal ultrasound; 5 of the 14 infants underwent a lumbar magnetic resonance imaging. One infant had urodynamics studies and a tethered cord release. Pediatricians should be familiar with TCS and perform lumbar physical examinations for LsCMs suggestive of TCS to ensure prompt diagnosis and management and avoid potentially devastating complications.
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Spiridigliozzi, Luca, Elisabetta Di Bartolomeo, Gianfranco Dell’Agli, and Francesca Zurlo. "GDC-Based Infiltrated Electrodes for Solid Oxide Electrolyzer Cells (SOECs)." Applied Sciences 10, no. 11 (June 3, 2020): 3882. http://dx.doi.org/10.3390/app10113882.
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In this work, porous complex and metal-free cathodes based on a (La0.6Sr0.4) (Cr0.5Mn0.5) O3 (LSCM) screen-printed backbone infiltrated with Ce0.9Gd0.1O2 (GDC) were fabricated for solid oxide electrolyzer cells. GDC infiltration has been optimized by structural and microstructural investigation and tested by electrochemical measurements in CO/CO2 mixtures. Infiltrated electrodes with a non-aqueous GDC solution showed the best electro-catalytic activity towards CO2 reduction, exhibiting a much lower polarization resistance, i.e., Rpol = 0.3 Ω·cm2 at 900 °C. The electrochemical performance of LSCM/GDCE in terms of Rpol is comparable to the best-performing Ni-YSZ cathode in the same operating conditions (Rpol = 0.23 Ω·cm2).
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Li, Rui, Xiu Hua Chen, Wen Hui Ma, Jie Xing, Jian Jun Yang, and Jie Yu. "The Preparation Research for IT-SOFC Anode Material LSCM." Advanced Materials Research 581-582 (October 2012): 529–32. http://dx.doi.org/10.4028/www.scientific.net/amr.581-582.529.
Full textAbstract:
Perovskite-structure La0.7Sr0.3Cr0.5Mn0.5O3-δanode powder was prepared by glycine nitrate process. The result of characterization indicated that the pore morphology and mechanical property of anode support pellets using starch as pore-forming agent are superior to that of using activated carbon. The maximum value of porosity and specific surface area are 40%, 1.256m2/mg at 10MPa, respectively. The conductive mechanism is small-polaron conductive mechanism at low temperature, but it is metalloid conductive mechanism at high temperature.
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Lay, E., L. Dessemond, and G. Gauthier. "Ba-substituted LSCM anodes for solid oxide fuel cells." Journal of Power Sources 221 (January 2013): 149–56. http://dx.doi.org/10.1016/j.jpowsour.2012.07.126.
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Jung, Inyong, Daehee Lee, Seong Oh Lee, Dongha Kim, Joosun Kim, Sang-Hoon Hyun, and Jooho Moon. "LSCM–YSZ nanocomposites for a high performance SOFC anode." Ceramics International 39, no. 8 (December 2013): 9753–58. http://dx.doi.org/10.1016/j.ceramint.2013.05.022.
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