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Relevant bibliographies by topics / Luciferase reporter assays

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Academic literature on the topic 'Luciferase reporter assays'

Author: Grafiati

Published: 4 June 2021

Last updated: 9 February 2022

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Journal articles on the topic "Luciferase reporter assays"

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Kenda, Maša, Jan Vegelj, Barbara Herlah, Andrej Perdih, Přemysl Mladěnka, and Marija Sollner Dolenc. "Evaluation of Firefly and Renilla Luciferase Inhibition in Reporter-Gene Assays: A Case of Isoflavonoids." International Journal of Molecular Sciences 22, no. 13 (2021): 6927. http://dx.doi.org/10.3390/ijms22136927.

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Firefly luciferase is susceptible to inhibition and stabilization by compounds under investigation for biological activity and toxicity. This can lead to false-positive results in in vitro cell-based assays. However, firefly luciferase remains one of the most commonly used reporter genes. Here, we evaluated isoflavonoids for inhibition of firefly luciferase. These natural compounds are often studied using luciferase reporter-gene assays. We used a quantitative structure–activity relationship (QSAR) model to compare the results of in silico predictions with a newly developed in vitro assay that

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Lai, Chunfai, Xin Jiang, and Xianqiang Li. "Development of Luciferase Reporter-Based Cell Assays." ASSAY and Drug Development Technologies 4, no. 3 (2006): 307–15. http://dx.doi.org/10.1089/adt.2006.4.307.

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Paguio, Aileen, Pete Stecha, Keith V. Wood, and Frank Fan. "Improved Dual-Luciferase Reporter Assays for Nuclear Receptors." Current Chemical Genomics 4 (May 26, 2010): 43–49. http://dx.doi.org/10.2174/1875397301004010043.

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Roelant, Christiaan H., David A. Burns, and Winfried Scheirer. "Accelerating the Pace of Luciferase Reporter Gene Assays." BioTechniques 20, no. 5 (1996): 914–17. http://dx.doi.org/10.2144/96205pf01.

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Kolb, Alfred J., and Kenneth Neumann. "Luciferase Measurements in High Throughput Screening." Journal of Biomolecular Screening 1, no. 2 (1996): 85–88. http://dx.doi.org/10.1177/108705719600100207.

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Luminescence assays are becoming more popular in high throughput screening (HTS) laboratories with the luciferase reporter gene being the most common. As with other assays that are adapted to HTS, improvements have been made to the luciferase assay to make it better suited to the requirements of HTS. For the luciferase reporter gene, these improvements included stabilization of the enzyme, increasing the half-life of the luminescence signal to 5 h, and eliminating separation steps (centrifugation and aliquot transfer) after cell lysis. The improved assay, LucLite, is homogeneous and is measure

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Hancock, Michael K., Myleen N. Medina, Brendan M. Smith, and Anthony P. Orth. "Microplate Orbital Mixing Improves High-Throughput Cell-Based Reporter Assay Readouts." Journal of Biomolecular Screening 12, no. 1 (2006): 140–44. http://dx.doi.org/10.1177/1087057106296046.

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Reporter assays are commonly used for high-throughput cell-based screening of compounds, cDNAs, and siRNAs due to robust signal, ease of miniaturization, and simple detection and analysis. Among the most widely used reporter genes is the bioluminescent enzyme luciferase, which, when exposed to its substrate luciferin upon cell lysis, yields linear signal over a dynamic range of several orders of magnitude. Commercially available luciferase assay formulations have been developed permitting homogeneous, single-step cell lysis and reporter activity measurements. Assay conditions employed with the

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Didiot, Marie-Cecile, Sergio Serafini, Martin J. Pfeifer, Frederick J. King, and Christian N. Parker. "Multiplexed Reporter Gene Assays: Monitoring the Cell Viability and the Compound Kinetics on Luciferase Activity." Journal of Biomolecular Screening 16, no. 7 (2011): 786–93. http://dx.doi.org/10.1177/1087057111407768.

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High-throughput screening assays with multiple readouts enable one to monitor multiple assay parameters. By capturing as much information about the underlying biology as possible, the detection of true actives can be improved. This report describes an extension to standard luciferase reporter gene assays that enables multiple parameters to be monitored from each sample. The report describes multiplexing luciferase assays with an orthogonal readout monitoring cell viability using reduction of resazurin. In addition, this technical note shows that by using the luciferin substrate in live cells,

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Repele, Andrea, and Manu. "Robust Normalization of Luciferase Reporter Data." Methods and Protocols 2, no. 3 (2019): 62. http://dx.doi.org/10.3390/mps2030062.

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Transient Luciferase reporter assays are widely used in the study of gene regulation and intracellular cell signaling. In order to control for sample-to-sample variation in luminescence arising from variability in transfection efficiency and other sources, an internal control reporter is co-transfected with the experimental reporter. The luminescence of the experimental reporter is normalized against the control by taking the ratio of the two. Here we show that this method of normalization, “ratiometric”, performs poorly when the transfection efficiency is low and leads to biased estimates of

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Siebring-van Olst, Ellen, Christie Vermeulen, Renee X. de Menezes, Michael Howell, Egbert F. Smit, and Victor W. van Beusechem. "Affordable Luciferase Reporter Assay for Cell-Based High-Throughput Screening." Journal of Biomolecular Screening 18, no. 4 (2012): 453–61. http://dx.doi.org/10.1177/1087057112465184.

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The firefly luciferase gene is commonly used in cell-based reporter assays. Convenient luciferase assay reagents for use in high-throughput screening (HTS) are commercially available. However, the high cost of these reagents is not within the means of some academic laboratories. Therefore, we set out to develop an affordable luciferase assay reagent applicable in an HTS format using simple liquid-handling steps. The reagent was homemade from individual chemical components and optimized for luminescence intensity and stability. We determined the minimal concentrations of the most expensive comp

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Braeuning, Albert, та Silvia Vetter. "The nuclear factor κB inhibitor (E)-2-fluoro-4′-methoxystilbene inhibits firefly luciferase". Bioscience Reports 32, № 6 (2012): 531–37. http://dx.doi.org/10.1042/bsr20120043.

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Photinus pyralis (firefly) luciferase is widely used as a reporter system to monitor alterations in gene promoter and/or signalling pathway activities in vitro. The enzyme catalyses the formation of oxyluciferin from D-luciferin in an ATP-consuming reaction involving photon emission. The purpose of the present study was to characterize the luciferase-inhibiting potential of (E)-2-fluoro-4′-methoxystilbene, which is known as a potent inhibitor of the NF-κB (nuclear factor κB) signalling pathway that is used to modulate the NF-κB signalling pathway in vitro. Results show that (E)-2-fluoro-4′-met

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Dissertations / Theses on the topic "Luciferase reporter assays"

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Dukorn, Stefanie [Verfasser], та A. [Akademischer Betreuer] Buschauer. "Pharmacological tools for the NPY receptors: [³⁵S]GTPγS binding assays, luciferase gene reporter assays and labeled peptides / Stefanie Dukorn ; Betreuer: A. Buschauer". Regensburg : Universitätsbibliothek Regensburg, 2018. http://d-nb.info/115170007X/34.

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Wiencierz, Anne Maria. "Entwicklung eines Dual-Luciferase-Reportergen-Assays zum Nachweis der Induktion antioxidativer Enzyme durch Nahrungsbestandteile." Master's thesis, Universität Potsdam, 2008. http://opus.kobv.de/ubp/volltexte/2009/2790/.

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Die Induktion antioxidativer Enzyme gilt als eine Möglichkeit, die antioxidative Kapazität von Zellen zu steigern und dadurch mit oxidativem Stress assoziierten Erkrankungen (z. B. Herz-Kreislauf-Erkrankungen, Neurodegeneration, Atherosklerose) vorzubeugen. Ausgehend davon wurde in der vorliegenden Arbeit der Dual-Luciferase-Reportergen-(DLR)-Assay zum Nachweis der Induktion der antioxidativen Enzyme Katalase (CAT), zytosolische Glutathion-Peroxidase (GPX1) und Kupfer-Zink-Superoxid-Dismutase (SOD1) entwickelt. Im Zuge dessen wurden drei Säugetierzelllinien (CaCo2, IEC-18, V79) auf ihre Eignun

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Douvris, Adrianna. "Functional Analysis of the TRIB1 Locus in Coronary Artery Disease." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20115.

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The TRIB1 locus (8q24.13) is a novel locus associated with plasma TGs and CAD risk. Trib1 is a regulator of MAPK activity, and has been shown to regulate hepatic lipogenesis and VLDL production in mice. However, the functional relationship between common SNPs at the TRIB1 locus and plasma lipid traits is unknown; TRIB1 has not been identified as an eQTL. This cluster of SNPs falls within an intergenic region 25kb to 50kb downstream of the TRIB1 coding region. By phylogenetic footprinting analysis and DNA genotyping, we identified an evolutionarily conserved region (CNS1) within the risk locus

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Zheng, Qiaoyun. "Study of Cancer Related Proteins: LRG-1 and PD-L1." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1495735827467521.

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Lai, John. "Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16454/.

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This PhD aimed to elucidate the mechanisms by which polymorphisms may alter androgen-induced transactivation of androgen receptor (AR) target genes which may be important in prostate cancer aetiology. The second aspect of this PhD focused on identifying and characterising functional polymorphisms that may have utility as predictive risk indicators for prostate cancer and which may aid in earlier therapeutic intervention and better disease management. Analyses were carried out on the kallikrein-related peptidase 3 (KLK3), also known as the prostate specific antigen (PSA), gene and the kallikrei

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Hofmann, Peter Josef. "Aufbau eines Reportergenassays zur Untersuchung der Wechselwirkung endokriner Disruptoren mit der T 3-regulierten Transaktivierung." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2008. http://dx.doi.org/10.18452/15813.

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Das Schilddrüsenhormon Triiodthyronin (T3) ist ein essenzieller Regulator physiologischer Prozesse der Entwicklung, des Wachstums und im Intermediärstoffwechsel. Täglich werden zahlreiche natürliche und synthetische Stoffe aufgenommen, die mit dem endokrinen System interferieren und deshalb als Endokrine Disruptoren (ED) bezeichnet werden. Zur Untersuchung einer direkten Interferenz von ED mit den Schilddrüsenhormonrezeptoren (TR) und ihrer transkriptionellen Aktivität wurde im Rahmen dieser Arbeit ein neues Luziferase-basiertes T3 Reportergensystem mit TRalpha1-transfizierten humanen Leberzel

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Lu, Yu-Jou, and 陸羽柔. "Analysis of HHP1 Promoter Using Luciferase Reporter Assay System." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/54762340551517489882.

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碩士<br>國立臺灣大學<br>生化科技學系<br>99<br>HHP1 (heptahelical transmembrane protein 1), a protein with a predicted seven transmembrane domain structure homologous to mPRs (membrane progestin receptors), is determined to be a novel negative regulator in ABA and osmotic signaling. Sequence analysis showed that the fragment upstream to the coding region of HHP1 (5’ UTR + -1 ~ -1552 bp) contains many ABA-, dehydration-, salinity- and low temperature-responsive elements. In this study, we tried to investigate the stress-responsive elements which are involved in HHP1 transcriptional regulation using Dual-Lu

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Lin, Yun-Tong, and 林筠彤. "The small RNA of Japanese encephalitis virus inhibits translation efficiency analyzed by a luciferase reporter gene assay." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/82w7a2.

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碩士<br>國立東華大學<br>生物技術研究所<br>96<br>Japanese encephalitis virus (JEV) contains a single positive-strand RNA genome of 10,976 nucleotides in length. The 5’- and 3’-terminal regions contain conserved and highly ordered secondary structures that have been shown to play important roles in viral translation and replication. Previous studies in our laboratory have shown the abundant accumulation of a 3’-terminal 521- to 523-nucleotide genome fragment, representing the highly conserved region of the 3’-untranslated region, in JEV-infected cells. Function of the small RNA remains unclear. The small R

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Pohlová, Lenka. "Onkogenní promotor c-myc jako cíl pro nový typ heterocyklických dikationtů stabilizujících G-kvadruplex." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-353836.

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Targeting oncogene promoters: a novel heterocyclic cations as G-quadruplex stabilizing ligands Lenka Pohlová Abstract: The diploma thesis studies an effect of newly synthesized group of compounds - helquats - on the expression of c-myc as a major player in malignant transformation and tumorigenesis via the stabilization of G-quadruplex in c-myc promotor. The G-quadruplex c-myc stabilization ability was tested for 101 helquats using dual luciferase reporter assay. The G-quadruplex c-myc stabilization ability was found for 13 helquats by this method. 8 successful helquats was selected by a compa

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Kožantová, Jana. "Měření aktivace signálních drah v myší makrofágové linii IC-21 a primárních dendritických buňkách po infekci virem klíšťové encefalitidy." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-367677.

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Tick-borne encephalitis is a serious disease of the central nervous system. It is caused by tick-borne encephalitis virus, which is transmitted by ticks. The Czech Republic is one of the countries with the highest prevalence of this disease. Tick-borne encephalitis virus is able to replicate in several cell types. In this work we focused on macrophage line IC-21 and dendritic cells, because these cells are the first, which encounter the virus and support its spreading in the host at early stage of infection. So far there is not known any specific receptor for virus entry into cells or which si

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Book chapters on the topic "Luciferase reporter assays"

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Bauer, Paul. "Luciferase Reporter Gene Assays." In Encyclopedia of Cancer. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-16483-5_3430.

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Bauer, Paul. "Luciferase Reporter Gene Assays." In Encyclopedia of Cancer. Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27841-9_3430-2.

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Bauer, Paul. "Luciferase Reporter Gene Assays." In Encyclopedia of Cancer. Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-46875-3_3430.

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Siebring-van Olst, Ellen, and Victor W. van Beusechem. "High-Throughput Firefly Luciferase Reporter Assays." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7724-6_2.

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Gage, Matthew C., Benoit Pourcet, and Inés Pineda-Torra. "Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3170-5_7.

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Clément, Thomas, Véronique Salone, and Mathieu Rederstorff. "Dual Luciferase Gene Reporter Assays to Study miRNA Function." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2547-6_17.

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Dupont, Sirio. "Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8910-2_11.

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Nair, Anup K., and Leslie J. Baier. "Using Luciferase Reporter Assays to Identify Functional Variants at Disease-Associated Loci." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7471-9_17.

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Hölting, Tilman L. B., and Maximilian M. L. Knott. "Analysis of Regulatory DNA Sequences by Dual-Luciferase Reporter Assays in Ewing Sarcoma." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1020-6_10.

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Pandolfi, Silvia, and Barbara Stecca. "Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Cancer Cells." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2772-2_7.

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Conference papers on the topic "Luciferase reporter assays"

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Liu, Haiyun, Brian Della Fera, John Foulke, Luping Chen, and Fang Tian. "Abstract 1306: Primary NK cells and luciferase expressing reporter cell lines for use in developing ADCC assays for immuno-oncology drug screening." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-1306.

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Shi, Yihui, Jaehyeon Park, Chandraiah Lagisetti, Wei Zhou, Lidia C. Sambucetti, and Thomas R. Webb. "Abstract 4193: A triple exon-skipping luciferase reporter assay identifies a new CLK inhibitor scaffold." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-4193.

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Fields, Wanda R., Wolfgang Liedtke, Jinju Li, and Betsy Bombick. "Abstract 1693: Assessment of Nrf2 luciferase reporter assay and heme oxygenase expression as models of oxidative stress inin vitrocultures of lung cells exposed to cigarette smoke condensate." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-1693.

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