Academic literature on the topic 'Luciferin-luciferase bioluminescence'
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Journal articles on the topic "Luciferin-luciferase bioluminescence"
Simonyan, Hayk, Chansol Hurr, and Colin N. Young. "A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions." Physiological Genomics 48, no. 10 (October 1, 2016): 762–70. http://dx.doi.org/10.1152/physiolgenomics.00055.2016.
Full textKotlobay, Alexey A., Maxim A. Dubinnyi, Konstantin V. Purtov, Elena B. Guglya, Natalja S. Rodionova, Valentin N. Petushkov, Yaroslav V. Bolt, et al. "Bioluminescence chemistry of fireworm Odontosyllis." Proceedings of the National Academy of Sciences 116, no. 38 (August 28, 2019): 18911–16. http://dx.doi.org/10.1073/pnas.1902095116.
Full textSaito-Moriya, Ryohei, Jun Nakayama, Genta Kamiya, Nobuo Kitada, Rika Obata, Shojiro A. Maki, and Hiroshi Aoyama. "How to Select Firefly Luciferin Analogues for In Vivo Imaging." International Journal of Molecular Sciences 22, no. 4 (February 12, 2021): 1848. http://dx.doi.org/10.3390/ijms22041848.
Full textYull, Fiona E., Wei Han, E. Duco Jansen, M. Brett Everhart, Ruxana T. Sadikot, John W. Christman, and Timothy S. Blackwell. "Bioluminescent Detection of Endotoxin Effects on HIV-1 LTR-driven Transcription in Vivo." Journal of Histochemistry & Cytochemistry 51, no. 6 (June 2003): 741–49. http://dx.doi.org/10.1177/002215540305100605.
Full textDesjardins, Michel, and David Morse. "The polypeptide components of scintillons, the bioluminescence organelles of the dinoflagellate Gonyaulax polyedra." Biochemistry and Cell Biology 71, no. 3-4 (March 1, 1993): 176–82. http://dx.doi.org/10.1139/o93-028.
Full textJones, Krysten A., William B. Porterfield, Colin M. Rathbun, David C. McCutcheon, Miranda A. Paley, and Jennifer A. Prescher. "Orthogonal Luciferase–Luciferin Pairs for Bioluminescence Imaging." Journal of the American Chemical Society 139, no. 6 (February 3, 2017): 2351–58. http://dx.doi.org/10.1021/jacs.6b11737.
Full textViviani, Vadim R., Vanessa R. Bevilaqua, Daniel R. de Souza, Gabriel F. Pelentir, Michio Kakiuchi, and Takashi Hirano. "A Very Bright Far-Red Bioluminescence Emitting Combination Based on Engineered Railroad Worm Luciferase and 6′-Amino-Analogs for Bioimaging Purposes." International Journal of Molecular Sciences 22, no. 1 (December 30, 2020): 303. http://dx.doi.org/10.3390/ijms22010303.
Full textEndo, Mizuki, and Takeaki Ozawa. "Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission." International Journal of Molecular Sciences 21, no. 18 (September 7, 2020): 6538. http://dx.doi.org/10.3390/ijms21186538.
Full textOsipova, Z. M., A. S. Shcheglov, and I. V. Yampolsky. "A bioluminescent system of fungi: prospects for application in medical research." Alternatives to antibiotics, no. (1)2018 (March 4, 2018): 74–77. http://dx.doi.org/10.24075/brsmu.2018.004.
Full textShi, Ce, Michael P. Killoran, Mary P. Hall, Paul Otto, Monika G. Wood, Ethan Strauss, Lance P. Encell, Thomas Machleidt, Keith V. Wood, and Thomas A. Kirkland. "5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems." PLOS ONE 15, no. 12 (December 14, 2020): e0243747. http://dx.doi.org/10.1371/journal.pone.0243747.
Full textDissertations / Theses on the topic "Luciferin-luciferase bioluminescence"
Chan, Wai Shing. "Applications of the bacterial luciferin-luciferase system." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1454.
Full textOliveira, Anderson Garbuglio de. "Estudo mecanístico da bioluminescência de fungos." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/46/46135/tde-08112010-093327/.
Full textThis thesis describes how in vitro light emission can be enzymatically obtained from the hot and cold extracts assay using different species of fungi, which also indicates a common mechanism of light emission for all these organisms. Kinetic data suggest a consecutive two-step mechanism and corroborate the 1960\'s enzymatic proposal of Airth and Foerster. Finally, using hot and cold extracts assay we were also able to purify and to determine the molecular weight of the fungal luciferin (298.1837 m/z). The isolated substance emits light enzymatically in vitro, whose light emission spectrum matches with the fungal bioluminescence one thus confirming that the substance is the fungal luciferin
Pereira, Tatiana Araujo. "Purificação e caracterização de enzimas envolvidas na bioluminescência de fungos." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-12042018-105954/.
Full textThis work describes studies performed to purify and characterize enzymes responsible for the fungal bioluminescence. Also, it shows important data that contributes to understand the mechanism for bioluminescence reaction in fungi. First, we tried to isolate two enzymes suspected of being involved on fungal bioluminescence. Optimum activity parameters (pH and temperature) and kinetic behavior were investigated. However, the discovery that fungal luciferin is the hispidin derivative 3-hydroxyhispidin demanded adaptations in the project. First of all, concentrates efforts to luciferase purification was priority, since hydroxylase is not part of the bioluminescent system of fungi. Studies on the luciferase interaction with different substrates showed some promiscuity for the enzyme. The results indicated higher intensity of light from luciferin-luciferase reaction in alkaline solutions (pH ~ 8) at ~ 20 °C. The reaction in medium with 18O2 revealed that insertion of oxygen into the luciferin structure produces an intermediate whose decarboxylation generates oxyluciferin. In parallel, the in vitro synthesis of hispidin using caffeic acid and malonyl-CoA with the mycelium extract resulted in the emission of light, confirming that luciferin is recycled in the process.
Adams, Spencer T. Jr. "Deconstructing bioluminescence: from molecular detail to in vivo imaging." eScholarship@UMMS, 2020. https://escholarship.umassmed.edu/gsbs_diss/1064.
Full textBollinger, Robert Albin. "Evaluation of the light emission kinetics in luciferin/luciferase-based in vivo bioluminescence imaging for guidance in the development of small animal imaging study design." 2006. http://edissertations.library.swmed.edu/pdf/BollingerR051506/BollingerRobert.pdf.
Full textTan, Ju Jing. "Mechanosensitive ATP release in the lungs." Thesis, 2019. http://hdl.handle.net/1866/24849.
Full textATP is widely known to be an energy carrier within cells, but outside of the cell, it acts as an extracellular signaling molecule. Upon binding to purinergic receptors, extracellular ATP initiates the purinergic signaling to regulate certain physiological and pathophysiological processes. In the lungs, ATP stimulates surfactant secretion and promotes mucociliary clearance. Given the critical role of extracellular ATP in the lungs, it is important to understand the mechanism of cellular ATP release — the first step of purinergic signaling. Because mechanical forces constitute the primary trigger of ATP release, this thesis aims to investigate the physiological mechanism(s) and cellular sources of such mechanosensitive ATP release. This work is divided into three parts: 1) To study the spatial and temporal characteristics of ATP release, I developed a highly sensitive imaging technique based on luciferin-luciferase bioluminescence coupled with a custom-designed lens system, which combined a wide field of view (WFOV) and high light-gathering power. To evaluate our imaging approach, I subjected A549 cells, derived from human lung adenocarcinoma, to stretch or 50% hypotonic shock to trigger ATP release. I demonstrated that our technique allows us to precisely quantify the amount and the rate (or efflux) of ATP escaping from cells. The WFOV constitutes an essential tool used in the studies described in this thesis to determine the mechanism and cellular source of ATP release in the alveolus. 2) To examine the physiological mechanism of stretch-induced ATP release in primary alveolar cells, I determined the individual contributions of alveolar type 1 (AT1) in comparison with alveolar type 2 (AT2) cells. To this end, freshly isolated AT2 cells from rat lungs were seeded on a flexible silicone chamber and were cultured for up to seven days, which allowed AT2 cells to progressively transdifferentiate into AT1-like cells. The ratio of alveolar cells (AT2:AT1), being 4:1 on day 3, became 1:4 on day 7. The quantity of released ATP decreased with the decreasing numbers of AT2 cells, implicating them as the main source of ATP release in response to stretch. While pharmacological ATP channel modulators, carbenoxolone and probenecid, did not diminish the amount of ATP release, BAPTA, an intracellular calcium ([Ca2+]i) chelator, significantly reduced it. Likewise, these three modulators had similar effects on intracellular calcium responses measured by Fura-2, suggesting a connection between ATP release and [Ca2+]i levels. 3) To explore the role of membrane viscoelastic properties in mechanosensitive ATP release, I demonstrated that a 30% strain induced transient ATP release that was accompanied by uptake of propidium iodide (PI) in AT2 cells. This is consistent with a strain-induced transient membrane rupture, big enough for the passage of ATP and PI. ATP efflux also increases with strain rate, and hold time prolongs the half-life of ATP release. Thus, these results provide clues on how stretching of the viscoelastic membrane may lead to ATP release via an alternate mechanism involving transient mechanoporation of the cell membrane. Overall, these findings demonstrate that stretch-induced ATP release does not occur through ATP-conducting channels but rather a transient membrane mechanoporation. Further studies on membrane injury induced by strain are needed to better understand its contribution to mechanosensitive ATP release and [Ca2+]i signaling. Such studies will elucidate purinergic signaling in organs that are constantly exposed to physical stresses. This could suggest novel therapeutic targets/approach to modulate the negative impacts of excessive ATP release observed under certain pathological conditions, such as ventilator-induced lung injury.
Book chapters on the topic "Luciferin-luciferase bioluminescence"
Brovko, L. Yu, E. Yu Cherednikova, A. Yu Chikishev, N. L. Koroteev, and N. N. Ugarova. "Light-Induced Intramolecular Dynamics in Luciferin-Luciferase Bioluminescent System." In Spectroscopy of Biological Molecules: Modern Trends, 175–76. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5622-6_78.
Full textSaito-Moriya, Ryohei, Rika Obata, and Shojiro A. Maki. "Near-Infrared Luciferin Analogs for In Vivo Optical Imaging." In Bioluminescence [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96760.
Full textNishihara, Ryo, Kazuki Niwa, Tatsunosuke Tomita, and Ryoji Kurita. "Design of Coelenterazine Analogue to Reveal Bioluminescent Reaction of Human Serum Albumin." In Bioluminescence [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97232.
Full textMirza, Jeremy, and Yuichi Oba. "Semi-Intrinsic Luminescence in Marine Organisms." In Bioluminescence [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99369.
Full textWoodland Hastings, J., and Jay C. Dunlap. "[28] Cell-free components in dinoflagellate bioluminescence. The particulate activity: Scintillons; the soluble components: Luciferase, luciferin, and luciferin-binding protein." In Methods in Enzymology, 307–27. Elsevier, 1986. http://dx.doi.org/10.1016/0076-6879(86)33077-5.
Full textConference papers on the topic "Luciferin-luciferase bioluminescence"
ITO, KATSUTOSHI, KAZUTO NAKAGAWA, SEIJI MURAKAMI, HIDETOSHI ARAKAWA, and MASAKO MAEDA. "DEVELOPMENT OF SIMULTANEOUS BIOLUMINESCENT ASSAY OF ACETATE KINASE AND PYRUVATE PHOSPHATE DIKINASE USING FIREFLY LUCIFERASE-LUCIFERIN REACTION." In Bioluminescence and Chemiluminescence - Progress and Current Applications - 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL). WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812776624_0102.
Full textMiyashita, Y., and M. Iwasaka. "Luciferin-luciferase bioluminescent emitting in the suspension of dia-magnetically aligned guanine microcrystals." In 2015 IEEE International Magnetics Conference (INTERMAG). IEEE, 2015. http://dx.doi.org/10.1109/intmag.2015.7157577.
Full textARAKAWA, H., M. SHIOKAWA, O. IMAMURA, A. KOKADO, and M. MAEDA. "NEW BIOLUMINESCENT ASSAY OF ALKALINE PHOSPHATASE USING ADENOSINE-3'-PHOSPHATE-5'-PHOSPHOSULFATE AS SUBSTRATE AND LUCIFERIN LUCIFERASE REACTION." In Proceedings of the 11th International Symposium. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811158_0064.
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