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Journal articles on the topic 'Luciferin-luciferase bioluminescence'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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1

Simonyan, Hayk, Chansol Hurr, and Colin N. Young. "A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions." Physiological Genomics 48, no. 10 (2016): 762–70. http://dx.doi.org/10.1152/physiolgenomics.00055.2016.

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Bioluminescence imaging is an effective tool for in vivo investigation of molecular processes. We have demonstrated the applicability of bioluminescence imaging to spatiotemporally monitor gene expression in cardioregulatory brain nuclei during the development of cardiovascular disease, via incorporation of firefly luciferase into living animals, combined with exogenous d-luciferin substrate administration. Nevertheless, d-luciferin uptake into the brain tissue is low, which decreases the sensitivity of bioluminescence detection, particularly when considering small changes in gene expression i

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Kotlobay, Alexey A., Maxim A. Dubinnyi, Konstantin V. Purtov, et al. "Bioluminescence chemistry of fireworm Odontosyllis." Proceedings of the National Academy of Sciences 116, no. 38 (2019): 18911–16. http://dx.doi.org/10.1073/pnas.1902095116.

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Marine polychaetes Odontosyllis undecimdonta, commonly known as fireworms, emit bright blue-green bioluminescence. Until the recent identification of the Odontosyllis luciferase enzyme, little progress had been made toward characterizing the key components of this bioluminescence system. Here we present the biomolecular mechanisms of enzymatic (leading to light emission) and nonenzymatic (dark) oxidation pathways of newly described O. undecimdonta luciferin. Spectral studies, including 1D and 2D NMR spectroscopy, mass spectrometry, and X-ray diffraction, of isolated substances allowed us to ch

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Saito-Moriya, Ryohei, Jun Nakayama, Genta Kamiya, et al. "How to Select Firefly Luciferin Analogues for In Vivo Imaging." International Journal of Molecular Sciences 22, no. 4 (2021): 1848. http://dx.doi.org/10.3390/ijms22041848.

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Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly B

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Yull, Fiona E., Wei Han, E. Duco Jansen, et al. "Bioluminescent Detection of Endotoxin Effects on HIV-1 LTR-driven Transcription in Vivo." Journal of Histochemistry & Cytochemistry 51, no. 6 (2003): 741–49. http://dx.doi.org/10.1177/002215540305100605.

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We investigated the effects of Gram-negative bacterial lipopolysaccharide (LPS) on luciferase expression in transgenic reporter mice in which luciferase expression is driven by the nuclear factor κB (NF-κB)-dependent portion of the human immunodeficiency virus-1 (HIV-1) long terminal repeat (HIV-1 LTR). Using these mice, we dissected the sources of luciferase activity at the organ level by (a) assessing luciferase activity in organ homogenates, (b) bioluminescence imaging in vivo, and (c) bioluminescence imaging of individual organs ex vivo. Luciferin dosage was a critical determinant of the m

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Desjardins, Michel, and David Morse. "The polypeptide components of scintillons, the bioluminescence organelles of the dinoflagellate Gonyaulax polyedra." Biochemistry and Cell Biology 71, no. 3-4 (1993): 176–82. http://dx.doi.org/10.1139/o93-028.

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Scintillons, the bioluminescence organelles of Gonyaulax polyedra, were purified by isopycnic density gradient centrifugation until only low levels of contaminating chloroplasts and mitochondria were detected by fluorescence and electron microscopy. Purified scintillons catalyzed the luminescent reaction with kinetics identical to those observed during the bioluminescence flash in vivo. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate indicated that the organelles appeared to contain only two proteins. These proteins were identified as luciferase (135 kilodaltons) a

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Jones, Krysten A., William B. Porterfield, Colin M. Rathbun, David C. McCutcheon, Miranda A. Paley, and Jennifer A. Prescher. "Orthogonal Luciferase–Luciferin Pairs for Bioluminescence Imaging." Journal of the American Chemical Society 139, no. 6 (2017): 2351–58. http://dx.doi.org/10.1021/jacs.6b11737.

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Viviani, Vadim R., Vanessa R. Bevilaqua, Daniel R. de Souza, Gabriel F. Pelentir, Michio Kakiuchi, and Takashi Hirano. "A Very Bright Far-Red Bioluminescence Emitting Combination Based on Engineered Railroad Worm Luciferase and 6′-Amino-Analogs for Bioimaging Purposes." International Journal of Molecular Sciences 22, no. 1 (2020): 303. http://dx.doi.org/10.3390/ijms22010303.

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Beetle luciferases produce bioluminescence (BL) colors ranging from green to red, having been extensively used for many bioanalytical purposes, including bioimaging of pathogen infections and metastasis proliferation in living animal models and cell culture. For bioimaging purposes in mammalian tissues, red bioluminescence is preferred, due to the lower self-absorption of light at longer wavelengths by hemoglobin, myoglobin and melanin. Red bioluminescence is naturally produced only by Phrixothrix hirtus railroad worm luciferase (PxRE), and by some engineered beetle luciferases. However, Far-R

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Endo, Mizuki, and Takeaki Ozawa. "Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission." International Journal of Molecular Sciences 21, no. 18 (2020): 6538. http://dx.doi.org/10.3390/ijms21186538.

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In vivo bioluminescence imaging (BLI), which is based on luminescence emitted by the luciferase–luciferin reaction, has enabled continuous monitoring of various biochemical processes in living animals. Bright luminescence with a high signal-to-background ratio, ideally red or near-infrared light as the emission maximum, is necessary for in vivo animal experiments. Various attempts have been undertaken to achieve this goal, including genetic engineering of luciferase, chemical modulation of luciferin, and utilization of bioluminescence resonance energy transfer (BRET). In this review, we overvi

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Osipova, Z. M., A. S. Shcheglov, and I. V. Yampolsky. "A bioluminescent system of fungi: prospects for application in medical research." Alternatives to antibiotics, no. (1)2018 (March 4, 2018): 74–77. http://dx.doi.org/10.24075/brsmu.2018.004.

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Bioluminescence is chemical oxidation of a small luciferin molecule by air catalyzed by luciferase and accompanied by the emission of photons in the visible spectrum. This reaction is used in bioluminescent bioimaging, the method for the visualization of organism’s interior. Bioimaging is a popular tool used in medical research. However, it has an unfortunate drawback: it requires introduction of external luciferin to the system before every experiment. In this work we discuss a possibility of developing an autonomous luminescent system in eukaryotes based on the bioluminescent system of highe

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Shi, Ce, Michael P. Killoran, Mary P. Hall, et al. "5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems." PLOS ONE 15, no. 12 (2020): e0243747. http://dx.doi.org/10.1371/journal.pone.0243747.

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Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings. Solutions of detection reagent, containing a mixture of a substrate and luciferase enzyme that produces photons in the presence of ATP, are relatively unstable and maintain only a limited shelf life even under refrigerated conditions. It is therefore common for the individual performing a hygiene test to manually prepare fresh reagent at the time of monitoring. To simplify sample processing, a liquid detection reagent with improved thermal

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Waldenmaier, Hans E., Anderson G. Oliveira, and Cassius V. Stevani. "Thoughts on the diversity of convergent evolution of bioluminescence on earth." International Journal of Astrobiology 11, no. 4 (2012): 335–43. http://dx.doi.org/10.1017/s1473550412000146.

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AbstractThe widespread independent evolution of analogous bioluminescent systems is one of the most impressive and diverse examples of convergent evolution on earth. There are roughly 30 extant bioluminescent systems that have evolved independently on Earth, with each system likely having unique enzymes responsible for catalysing the bioluminescent reaction. Bioluminescence is a chemical reaction involving a luciferin molecule and a luciferase or photoprotein that results in the emission of light. Some independent systems utilize the same luciferin, such as the use of tetrapyrrolic compounds b

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Tessler, Michael, Jean P. Gaffney, Jason M. Crawford, et al. "Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens." PeerJ 6 (September 14, 2018): e5506. http://dx.doi.org/10.7717/peerj.5506.

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Bioluminescent copepods are often the most abundant marine zooplankton and play critical roles in oceanic food webs. Metridia copepods exhibit particularly bright bioluminescence, and the molecular basis of their light production has just recently begun to be explored. Here we add to this body of work by transcriptomically profiling Metridia lucens, a common species found in temperate, northern, and southern latitudes. In this previously molecularly-uncharacterized species, we find the typical luciferase paralog gene set found in Metridia. More surprisingly, we recover noteworthy putative luci

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KrishnaMurthy, N. V., T. Sudhaharan, and A. Ram Reddy. "Dye induced quenching of firefly luciferase–luciferin bioluminescence." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 68, no. 3 (2007): 851–59. http://dx.doi.org/10.1016/j.saa.2007.01.003.

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14

Feeney, Kevin A., Marrit Putker, Marco Brancaccio, and John S. O’Neill. "In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells." Journal of Biological Rhythms 31, no. 6 (2016): 540–50. http://dx.doi.org/10.1177/0748730416668898.

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Firefly luciferase (Fluc) is frequently used to report circadian gene expression rhythms in mammalian cells and tissues. During longitudinal assays it is generally assumed that enzymatic substrates are in saturating excess, such that total bioluminescence is directly proportional to Fluc protein level. To test this assumption, we compared the enzyme kinetics of purified luciferase with its activity in mammalian cells. We found that Fluc activity in solution has a lower Michaelis constant (Km) for luciferin, lower temperature dependence, and lower catalytic half-life than Fluc in cells. In cons

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15

Gregor, Carola, Jasmin K. Pape, Klaus C. Gwosch, Tanja Gilat, Steffen J. Sahl, and Stefan W. Hell. "Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system." Proceedings of the National Academy of Sciences 116, no. 52 (2019): 26491–96. http://dx.doi.org/10.1073/pnas.1913616116.

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Bioluminescence-based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotemporal resolution. The bacterial bioluminescence system is fully genetically encodable and hence produces autonomous bioluminescence without an external luciferin, but its brightness in cell types other than bacteria has, so far, not been sufficient for imaging single cells. We coe

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16

Yeh, Hsien-Wei, Omran Karmach, Ao Ji, David Carter, Manuela M. Martins-Green, and Hui-wang Ai. "Red-shifted luciferase–luciferin pairs for enhanced bioluminescence imaging." Nature Methods 14, no. 10 (2017): 971–74. http://dx.doi.org/10.1038/nmeth.4400.

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17

Fajardo, Carlos, Marcos De Donato, Hectorina Rodulfo, et al. "New Perspectives Related to the Bioluminescent System in Dinoflagellates: Pyrocystis lunula, a Case Study." International Journal of Molecular Sciences 21, no. 5 (2020): 1784. http://dx.doi.org/10.3390/ijms21051784.

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Pyrocystis lunula is considered a model organism due to its bioluminescence capacity linked to circadian rhythms. The mechanisms underlying the bioluminescent phenomenon have been well characterized in dinoflagellates; however, there are still some aspects that remain an enigma. Such is the case of the presence and diversity of the luciferin-binding protein (LBP), as well as the synthesis process of luciferin. Here we carry out a review of the literature in relation to the molecular players responsible for bioluminescence in dinoflagellates, with particular interest in P. lunula. We also carri

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18

Miceli, Matteo, Silvana Casati, Pietro Allevi, et al. "A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase." International Journal of Molecular Sciences 22, no. 11 (2021): 6148. http://dx.doi.org/10.3390/ijms22116148.

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A novel bioluminescent Monoacylglycerol lipase (MAGL) substrate 6-O-arachidonoylluciferin, a D-luciferin derivative, was synthesized, physico-chemically characterized, and used as highly sensitive substrate for MAGL in an assay developed for this purpose. We present here a new method based on the enzymatic cleavage of arachidonic acid with luciferin release using human Monoacylglycerol lipase (hMAGL) followed by its reaction with a chimeric luciferase, PLG2, to produce bioluminescence. Enzymatic cleavage of the new substrate by MAGL was demonstrated, and kinetic constants Km and Vmax were dete

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19

Ikeda, Yuma, Tsuyoshi Saitoh, Kazuki Niwa, et al. "An allylated firefly luciferin analogue with luciferase specific response in living cells." Chemical Communications 54, no. 14 (2018): 1774–77. http://dx.doi.org/10.1039/c7cc09720d.

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20

Kotlobay, A. A., Z. M. Kaskova, and I. V. Yampolsky. "Palette of Luciferases: Natural Biotools for New Applications in Biomedicine." Acta Naturae 12, no. 2 (2020): 15–27. http://dx.doi.org/10.32607/actanaturae.10967.

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Optoanalytical methods based on using genetically encoded bioluminescent enzymes,luciferases, allow one to obtain highly sensitive signals, are non-invasive, and require no external irradiation. Bioluminescence is based on the chemical reaction of oxidation of a low-molecular-weight substrate (luciferin) by atmospheric oxygen, which is catalyzed by an enzyme (luciferase). Relaxation of the luciferin oxidation product from its excited state is accompanied by a release of a quantum of light, which can be detected as an analytical signal.The ability to express luciferase genes in various heterolo

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Kotlobay, A. A., Z. M. Kaskova, and I. V. Yampolsky. "Palette of Luciferases: Natural Biotools for New Applications in Biomedicine." Acta Naturae 12, no. 2 (2020): 15–27. http://dx.doi.org/10.32607/actanaturae.11152.

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Optoanalytical methods based on using genetically encoded bioluminescent enzymes,luciferases, allow one to obtain highly sensitive signals, are non-invasive, and require no external irradiation. Bioluminescence is based on the chemical reaction of oxidation of a low-molecular-weight substrate (luciferin) by atmospheric oxygen, which is catalyzed by an enzyme (luciferase). Relaxation of the luciferin oxidation product from its excited state is accompanied by a release of a quantum of light, which can be detected as an analytical signal.The ability to express luciferase genes in various heterolo

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22

Shao, C. Y., C. J. Howe, A. J. R. Porter, and L. A. Glover. "Novel Cyanobacterial Biosensor for Detection of Herbicides." Applied and Environmental Microbiology 68, no. 10 (2002): 5026–33. http://dx.doi.org/10.1128/aem.68.10.5026-5033.2002.

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ABSTRACT The aim of this work was to generate a cyanobacterial biosensor that could be used to detect herbicides and other environmental pollutants. A representative freshwater cyanobacterium, Synechocystis sp. strain PCC6803, was chromosomally marked with the luciferase gene luc (from the firefly Photinus pyralis) to create a novel bioluminescent cyanobacterial strain. Successful expression of the luc gene during growth of Synechocystis sp. strain PCC6803 cultures was characterized by measuring optical density and bioluminescence. Bioluminescence was optimized with regard to uptake of the luc

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23

Minekawa, Takayuki, Hiroshi Ohkuma, Katsushi Abe, Hiroaki Maekawa, and Hidetoshi Arakawa. "Practical application of bioluminescence enzyme immunoassay using enhancer for firefly luciferin-luciferase bioluminescence." Luminescence 26, no. 3 (2010): 167–71. http://dx.doi.org/10.1002/bio.1200.

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24

Vongsangnak, Wanwipa, Pramote Chumnanpuen, and Ajaraporn Sriboonlert. "Transcriptome analysis reveals candidate genes involved in luciferin metabolism inLuciola aquatilis(Coleoptera: Lampyridae)." PeerJ 4 (October 4, 2016): e2534. http://dx.doi.org/10.7717/peerj.2534.

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Bioluminescence, which living organisms such as fireflies emit light, has been studied extensively for over half a century. This intriguing reaction, having its origins in nature where glowing insects can signal things such as attraction or defense, is now widely used in biotechnology with applications of bioluminescence and chemiluminescence. Luciferase, a key enzyme in this reaction, has been well characterized; however, the enzymes involved in the biosynthetic pathway of its substrate, luciferin, remains unsolved at present. To elucidate the luciferin metabolism, we performed ade novotransc

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Jawhara, Samir, and Serge Mordon. "In Vivo Imaging of Bioluminescent Escherichia coli in a Cutaneous Wound Infection Model for Evaluation of an Antibiotic Therapy." Antimicrobial Agents and Chemotherapy 48, no. 9 (2004): 3436–41. http://dx.doi.org/10.1128/aac.48.9.3436-3441.2004.

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ABSTRACT A rapid, continuous method for noninvasively monitoring the effectiveness of several antibacterial agents in real time by using a model of wound infection was developed. This study was divided into three steps: (i) construction of a plasmid to transform Escherichia coli into a bioluminescent variant, (ii) study of the bioluminescent E. coli in vitro as a function of temperature and pH, and (iii) determination of the MIC and the minimal bactericidal concentration of sulfamethoxazole-trimethoprim (SMX-TMP). Finally, the efficacy of SMX-TMP was monitored in vivo in a cutaneous wound mode

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Kotlobay, Alexey A., Karen S. Sarkisyan, Yuliana A. Mokrushina, et al. "Genetically encodable bioluminescent system from fungi." Proceedings of the National Academy of Sciences 115, no. 50 (2018): 12728–32. http://dx.doi.org/10.1073/pnas.1803615115.

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Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Int

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Oba, Y., K. Konishi, D. Yano, H. Shibata, D. Kato, and T. Shirai. "Resurrecting the ancient glow of the fireflies." Science Advances 6, no. 49 (2020): eabc5705. http://dx.doi.org/10.1126/sciadv.abc5705.

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The color of firefly bioluminescence is determined by the structure of luciferase. Firefly luciferase genes have been isolated from more than 30 species, producing light ranging in color from green to orange-yellow. Here, we reconstructed seven ancestral firefly luciferase genes, characterized the enzymatic properties of the recombinant proteins, and determined the crystal structures of the gene from ancestral Lampyridae. Results showed that the synthetic luciferase for the last common firefly ancestor exhibited green light caused by a spatial constraint on the luciferin molecule in enzyme, wh

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Li, Shufeng, Zhiyang Ruan, Hang Zhang, and Haiwei Xu. "Recent achievements of bioluminescence imaging based on firefly luciferin-luciferase system." European Journal of Medicinal Chemistry 211 (February 2021): 113111. http://dx.doi.org/10.1016/j.ejmech.2020.113111.

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Niwa, Kazuki, Yoshihiro Nakajima, and Yoshihiro Ohmiya. "Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase." Analytical Biochemistry 396, no. 2 (2010): 316–18. http://dx.doi.org/10.1016/j.ab.2009.09.014.

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Wang, Anni, Xuewei Li, Yong Ju, Dongying Chen, and Jianzhong Lu. "Bioluminescence imaging of carbon monoxide in living cells based on a selective deiodination reaction." Analyst 145, no. 2 (2020): 550–56. http://dx.doi.org/10.1039/c9an02107h.

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Modification of a heavy iodine atom for d-Luciferin was explored as a “turn-on” transduction scheme for CO detection. This new probe could image exogenous and endogenous CO in the luciferase-transfected cancer cells.

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Delroisse, Jérôme, Esther Ullrich-Lüter, Stefanie Blaue, et al. "A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence." Open Biology 7, no. 4 (2017): 160300. http://dx.doi.org/10.1098/rsob.160300.

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Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes. Whereas luciferins can be shared by phylogenetically distant organisms which feed on organisms producing them, luciferases have been thought to be lineage-specific enzymes. Numerous light emission systems would then have co-emerged independently along the tree of life resulting in a plethora of non-homologous luciferases. Here, we identify for the first time a candidate luciferase of a luminou

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Zambito, Giorgia, Natasa Gaspar, Yanto Ridwan, et al. "Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging." Molecular Imaging and Biology 22, no. 6 (2020): 1523–31. http://dx.doi.org/10.1007/s11307-020-01523-7.

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Abstract Purpose Currently, a variety of red and green beetle luciferase variants are available for bioluminescence imaging (BLI). In addition, new luciferin analogues providing longer wavelength luminescence have been developed that show promise for improved deep tissue imaging. However, a detailed assessment of these analogues (e.g., Akalumine-HCl, CycLuc1, and amino naphthyl luciferin (NH2-NpLH2)) combined with state of the art luciferases has not been performed. The aim of this study was to evaluate for the first time the in vivo brightness and spectral characteristics of firefly (Luc2), c

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Fang, Zhongjian, Houchao Xu, Xiangjun Ji, et al. "Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements." Journal of Nanomaterials 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/8542806.

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The past two decades have witnessed the great growth of the development of novel drug carriers. However, the releasing dynamics of drug from drug carriers in vivo and the interactions between cells and drug carriers remain unclear. In this paper, liposomes were prepared to encapsulate D-luciferin, which was the substrate of luciferase and served as a model drug. Based on the theoretical calculation of active loading, methods of preparation for liposomes were optimized. Only when D-luciferin was released from liposomes or taken in by the cells could bioluminescence be produced under the catalys

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Hara, Kiyotaka Y., and Hideo Mori. "An Efficient Method for Quantitative Determination of Cellular ATP Synthetic Activity." Journal of Biomolecular Screening 11, no. 3 (2006): 310–17. http://dx.doi.org/10.1177/1087057105285112.

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The authors have developed an efficient method to measure cellular activity of ATP synthesis. Although ATP is a major energy source of biological reactions, it has been difficult tomeasure cellular ATP synthetic activity quantitatively. In this report, bioluminescence from the luciferin-luciferase reactionwas used for the quantitativemeasurement. Under the used condition, bioluminescence from standard ATP solution showed no attenuation within several minutes, and the intensity corresponded proportionally to ATP concentrations of the standards. To measure dynamic cellular ATP synthetic activity

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Yoshida, Masa-aki, Junichi Imoto, Yuri Kawai, et al. "Genomic and Transcriptomic Analyses of Bioluminescence Genes in the Enope Squid Watasenia scintillans." Marine Biotechnology 22, no. 6 (2020): 760–71. http://dx.doi.org/10.1007/s10126-020-10001-8.

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AbstractWatasenia scintillans, a sparkling enope squid, has bioluminescence organs to illuminate its body with its own luciferase activity. To clarify the molecular mechanism underlying its scintillation, we analysed high-throughput sequencing data acquired previously and obtained draft genome sequences accomplished with comparative genomic data among the cephalopods. The genome mapped by transcriptome data showed that (1) RNA editing contributed to transcriptome variation of lineage specific genes, such as W. scintillans luciferase, and (2) two types of luciferase enzymes were characterized w

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Bessho-Uehara, Manabu, Naoyuki Yamamoto, Shuji Shigenobu, Hitoshi Mori, Keiko Kuwata, and Yuichi Oba. "Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey." Science Advances 6, no. 2 (2020): eaax4942. http://dx.doi.org/10.1126/sciadv.aax4942.

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Through their diet, animals can obtain substances essential for imparting special characteristics, such as toxins in monarch butterflies and luminescent substances in jellyfishes. These substances are typically small molecules because they are less likely to be digested and may be hard for the consumer to biosynthesize. Here, we report that Parapriacanthus ransonneti, a bioluminescent fish, obtains not only its luciferin but also its luciferase enzyme from bioluminescent ostracod prey. The enzyme purified from the fish’s light organs was identical to the luciferase of Cypridina noctiluca, a bi

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Pajor, Katarzyna, Daniel Sypniewski, and Ilona Bednarek. "Bioluminescence as a tool in molecular biology." Postępy Higieny i Medycyny Doświadczalnej 71 (December 12, 2017): 0. http://dx.doi.org/10.5604/01.3001.0010.7011.

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Bioluminescence has been studied for many years by scientists. There are numerous mechanisms of that phenomenon; among them bacterial bioluminescence is the most frequently found in nature. This type of bioluminescence is determined by the appearance of lux operon, which encodes all elements necessary to produce light emission and it does not require any additional substrates supply. Another commonly found example of bioluminescence mechanism is performed by Photinus pyralis. Luciferase of P. pyralis named FLuc requires D-luciferin as a substrate. Bioluminescence is also characteristic for man

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Nakayama, Jun, Ryohei Saito, Yusuke Hayashi, et al. "High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai." International Journal of Molecular Sciences 21, no. 21 (2020): 7896. http://dx.doi.org/10.3390/ijms21217896.

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Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin anal

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Loimaranta, Vuokko, Jorma Tenovuo, Leeni Koivisto, and Matti Karp. "Generation of Bioluminescent Streptococcus mutans and Its Usage in Rapid Analysis of the Efficacy of Antimicrobial Compounds." Antimicrobial Agents and Chemotherapy 42, no. 8 (1998): 1906–10. http://dx.doi.org/10.1128/aac.42.8.1906.

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ABSTRACT The oral bacterium Streptococcus mutans was transformed by electroporation with a shuttle vector (pCSS945) containing insect luciferase gene from a click beetle (Pyrophorus plagiophthalamus) resulting in a bioluminescent phenotype. ThisS. mutans strain was used in experiments in which light emission was used as a rapid and, compared to conventional CFU counting, more convenient means of estimating the effects of various antimicrobial treatments. The basic parameters affecting in vivo light production by the strain were studied. It was found that pH 6.0 was optimal for incorporation of

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ZHENG, YING, QIAOYA LIN, HONGLIN JIN, JUAN CHEN, and ZHIHONG ZHANG. "VISUALIZATION OF HEAD AND NECK CANCER MODELS WITH A TRIPLE FUSION REPORTER GENE." Journal of Innovative Optical Health Sciences 05, no. 04 (2012): 1250028. http://dx.doi.org/10.1142/s1793545812500289.

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The development of experimental animal models for head and neck tumors generally rely on the bioluminescence imaging to achieve the dynamic monitoring of the tumor growth and metastasis due to the complicated anatomical structures. Since the bioluminescence imaging is largely affected by the intracellular luciferase expression level and external D-luciferin concentrations, its imaging accuracy requires further confirmation. Here, a new triple fusion reporter gene, which consists of a herpes simplex virus type 1 thymidine kinase (TK) gene for radioactive imaging, a far-red fluorescent protein (

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Lapointe, Mathieu, and David Morse. "Reassessing the role of a 3′-UTR-binding translational inhibitor in regulation of circadian bioluminescence rhythm in the dinoflagellate Gonyaulax." Biological Chemistry 389, no. 1 (2008): 13–19. http://dx.doi.org/10.1515/bc.2008.003.

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Abstract The nightly bioluminescence of the dinoflagellate Gonyaulax is a circadian rhythm caused by the presence in cells of specialized bioluminescent organelles, termed scintillons, containing the reaction catalyst luciferase, the substrate luciferin and a luciferin-binding protein (LBP). LBP levels increase at the start of the night phase because of increased protein synthesis rates in vivo, and this regulation has been ascribed to circadian binding of an inhibitory protein factor binding to the 3′ untranslated region (UTR) of lbp mRNA at times when LBP is not normally synthesized. To puri

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Heffern, Marie C., Hyo Min Park, Ho Yu Au-Yeung, et al. "In vivo bioluminescence imaging reveals copper deficiency in a murine model of nonalcoholic fatty liver disease." Proceedings of the National Academy of Sciences 113, no. 50 (2016): 14219–24. http://dx.doi.org/10.1073/pnas.1613628113.

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Copper is a required metal nutrient for life, but global or local alterations in its homeostasis are linked to diseases spanning genetic and metabolic disorders to cancer and neurodegeneration. Technologies that enable longitudinal in vivo monitoring of dynamic copper pools can help meet the need to study the complex interplay between copper status, health, and disease in the same living organism over time. Here, we present the synthesis, characterization, and in vivo imaging applications of Copper-Caged Luciferin-1 (CCL-1), a bioluminescent reporter for tissue-specific copper visualization in

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Gandelman, O. A., L. Yu Brovko, N. N. Ugarova, A. Yu Chikishev, and A. P. Shkurimov. "Oxyluciferin fluorescence is a model of native bioluminescence in the firefly luciferin—luciferase system." Journal of Photochemistry and Photobiology B: Biology 19, no. 3 (1993): 187–91. http://dx.doi.org/10.1016/1011-1344(93)87083-y.

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Mizuno, Gaku, Daichi Yano, José Paitio, Hiromitsu Endo, and Yuichi Oba. "Etmopterus lantern sharks use coelenterazine as the substrate for their luciferin-luciferase bioluminescence system." Biochemical and Biophysical Research Communications 577 (November 2021): 139–45. http://dx.doi.org/10.1016/j.bbrc.2021.09.007.

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Khalil, Ashraf A., Mark J. Jameson, William C. Broaddus, et al. "Subcutaneous Administration of D-Luciferin is an Effective Alternative to Intraperitoneal Injection in Bioluminescence Imaging of Xenograft Tumors in Nude Mice." ISRN Molecular Imaging 2013 (December 18, 2013): 1–7. http://dx.doi.org/10.1155/2013/689279.

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Currently, intraperitoneal (IP) injection of D-luciferin is the preferred method of providing substrate for bioluminescence imaging (BLI); however it has a failure rate of 3–10% due to accidental intestinal injection. The present study evaluates the quality of BLI after subcutaneous (SC) injection of D-luciferin and demonstrates the effectiveness of SC injection in anatomically disparate tumor models. Mice bearing luciferase-expressing tumors underwent BLI after SC or IP injection of D-luciferin. The average time to maximal luminescence was 6 min (range 5–9 min) after SC injection and 8 min (r

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SALA-NEWBY, Graciela B., Catherine M. THOMSON, and Anthony K. CAMPBELL. "Sequence and biochemical similarities between the luciferases of the glow-worm Lampyris noctiluca and the firefly Photinus pyralis." Biochemical Journal 313, no. 3 (1996): 761–67. http://dx.doi.org/10.1042/bj3130761.

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A full-length clone encoding Lampyris noctiluca (British glow-worm) luciferase was isolated from a complementary DNA (cDNA) expression library constructed with mRNA extracted from light organs. The luciferase was a 547-residue protein, as deduced from the nucleotide sequence. The protein was closely related to those of other lampyrid beetles, the similarity to Photinus pyralis luciferase being 84% and to Luciola 67%. In contrast, Lampyris luciferase had less sequence similarity to the luciferases of the click beetle Pyrophorus, at 48%. Engineering Lampyris luciferase in vitro showed that the C

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Barbaro, A., and S. Rożek. "Studies on luciferin-luciferase ATP assay in plants (etiolated wheat germs, and bean leaves)." Acta Societatis Botanicorum Poloniae 44, no. 3 (2015): 377–92. http://dx.doi.org/10.5586/asbp.1975.034.

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For ATP determination by the method of bioluminescence apparatus of home production was adapted from the equipment available in any isotope laboratory. The measurement error did not exceed 1.5 per cent. Methodical experiments concerned the choice of the extraction, fixation and storage methods of plant material for determination at the given moment of the amount of ATP in the tissues, unchanged by the analytical procedure. The highest ATP amounts were recovered by extraction with perchloric acid at high (25%) concentrations of the tissue in the homogenate. The best way of fixation of the mater

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Kieβling, M., K. Winsel, and B. Freytag. "Bioluminescence Reactions of Nucleotides Part 2: Investigations of AMP/ATP Solutions in the Luciferin/Luciferase System." Isotopenpraxis Isotopes in Environmental and Health Studies 27, no. 1 (1991): 42–44. http://dx.doi.org/10.1080/10256019108622462.

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Fukuba, Tatsuhiro, Takuroh Noguchi, Kei Okamura, and Teruo Fujii. "Adenosine Triphosphate Measurement in Deep Sea Using a Microfluidic Device." Micromachines 9, no. 8 (2018): 370. http://dx.doi.org/10.3390/mi9080370.

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Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L–L (luciferin–luciferase) assay with a miniature pumping unit and a photometry module for the measur

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Daydé, David, Florence Dommange, Stéphanie Lerondel, et al. "Quantitative Bioluminescent Method Appropriate for Bimodality Analysis To Improve and Follow a Syngenic Murine Model of B-Cell Lymphoma Expressing Human CD20." Blood 110, no. 11 (2007): 1400. http://dx.doi.org/10.1182/blood.v110.11.1400.1400.

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Abstract The anti-CD20 monoclonal antibody rituximab (C2B8) has shown promising results in the clinical treatment of patients with B-cell Non-Hodgkin’s Lymphoma (B-NHL). However, its therapeutic effect is variable whereas a better knowledge of factors affecting rituximab response could lead to improve its efficacy. It has been suggested that tumour burden could influence rituximab exposure and response in human. The lack of method to assess precisely tumour burden has however prevent to confirm such influence. Study of factors affecting antibody exposure requires bimodality analysis allowing t

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