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ROCHA, MARCELO SALLES, and FRANK RAYNNER V. RIBEIRO. "A new species of Pimelodus LaCépède, 1803 (Siluriformes: Pimelodidae) from rio Itacaiunas, rio Tocantins basin, Brazil." Zootaxa 2343, no. 1 (January 25, 2010): 57. http://dx.doi.org/10.11646/zootaxa.2343.1.5.
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Pimelodus luciae is described from rio Itacaiunas, rio Tocantins basin. The new species exhibits a unique spotted pigmentation pattern for Pimelodidae. Pimelodus luciae differs further from remaining valid Pimelodus species by the following combination of characters: the relatively short distance between the posterior nostril and the anterior orbital border; dorsal surface of the supraoccipital process rounded; horizontal orbital diameter greater than interorbital distance; and distal portion of dorsal fin hyaline. Pimelodus luciae is known only from the type-locality.
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Fournès, Christine. "Lucien Bailly (1871–1940): An eccentric troublemaker and a real precursor. A short story of shareholders’ activism in France at the beginning of the twentieth century." Accounting History 25, no. 4 (November 25, 2019): 518–35. http://dx.doi.org/10.1177/1032373219882434.
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The article highlights the role of an eccentric troublemaker at the beginning of the twentieth century – Lucien Bailly. The Pont-à-Mousson company’s archives, one of the major joint stock companies in the mining industry at that time, provide a wealth of information about this very interesting character. It is argued that Lucien Bailly paved the way for present-day activism. While the nature of shareholder-activists is far different today, there is a similar dichotomy between private and public or cooperative and hostile actions. This is the true legacy of Lucien Bailly. He was also the pioneer of proxy fights with the creation of the first association defending minority shareholders and the precursor of social activism.
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Milczanowski, Maciej. "Syndrom efektu Lucyfera w polityce międzykulturowej – wnioski dla Europy." Politeja 17, no. 3(66) (June 25, 2020): 199–210. http://dx.doi.org/10.12797/politeja.17.2020.66.14.
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Lucifer Effect Syndrome in the Intercultural Politics – Conclusions for Europe
Lucifer Effect is a process which progress gradually, slowly usually in mostly imperceptible way. That makes it especially dangerous because the society has just a little ability to identify its symptoms and phases. That is why, even if academics verify their concepts empirically it is always a crucial problem to warn the society of destructive conflict approaching. The paradox of conflict is that it is the fundamental for democracy (different opinions, minority rights etc.) and at the same time can be disastrous for this system and people living in. That paradox makes the “events horizon” very hard to determine and be aware of it. When society pass it, it can be too late to reconstruct the stability and security in short term, and even worst disasters happen. The aim of this text is to present the Lucifer Effect as the example realizing to the society the threats arising from the negative intercultural conflicts.
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Jeon, Ji-Hyeon, and Seung-Chul Kim. "Comparative Analysis of the Complete Chloroplast Genome Sequences of Three Closely Related East-Asian Wild Roses (Rosa sect. Synstylae; Rosaceae)." Genes 10, no. 1 (January 3, 2019): 23. http://dx.doi.org/10.3390/genes10010023.
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Species belonging to Rosa section Synstylae (Rosaceae) are mainly distributed in East Asia, and represent recently diverged lineages within the genus. Over decades, inferring phylogenetic relationships within section Synstylae have been exceptional challenges, due to short branch lengths and low support values. Of approximately 36 species in the section Synstylae, Rosa multiflora, Rosa luciae and Rosa maximowicziana are widely distributed in the Sino-Japanese floristic region. In this study, we assembled chloroplast genomes of these three species to compare the genomic features within section Synstylae, and to compare with other infrageneric groups. We found that three Rosa sect. Synstylae species had lost infA genes with pseudogenization, and they were almost identical to each other. Two protein-coding gene regions (ndhF and ycf1) and five non-coding regions (5’matK-trnK, psbI-trnS-trnG, rps16-trnG, rpoB-trnC, and rps4-trnT) were identified as being highly informative markers. Within three section Synstylae chloroplast genomes, 85 simple sequence repeat (SSR) motifs were detected, of which at least 13 motifs were identified to be effective markers. The phylogenetic relationships of R. multiflora, R. luciae and R. maximowicziana could not be resolved, even with chloroplast genome-wide data. This study reveals the chloroplast genomic data of Rosa sect. Synstylae, and it provides valuable markers for DNA barcoding and phylogenetic analyses for further studies.
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Vidyasagar, Sadasivan, Christian Barmeyer, John Geibel, Henry J. Binder, and Vazhaikkurichi M. Rajendran. "Role of short-chain fatty acids in colonic HCO3secretion." American Journal of Physiology-Gastrointestinal and Liver Physiology 288, no. 6 (June 2005): G1217—G1226. http://dx.doi.org/10.1152/ajpgi.00415.2004.
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Luminal isobutyrate, a relatively poor metabolized short-chain fatty acid (SCFA), induces HCO3secretion via a Cl-independent, DIDS-insensitive, carrier-mediated process as well as inhibiting both Cl-dependent and cAMP-induced HCO3secretion. The mechanism(s) responsible for these processes have not been well characterized. HCO3secretion was measured in isolated colonic mucosa mounted in Lucite chambers using pH stat technique and during microperfusion of isolated colonic crypts.14C-labeled butyrate,14C-labeled isobutyrate, and36Cl uptake were also determined by apical membrane vesicles (AMV) isolated from surface and/or crypt cells. Butyrate stimulation of Cl-independent, DIDS-insensitive 5-nitro-3-(3-phenylpropyl-amino)benzoic acid-insensitive HCO3secretion is greater than that by isobutyrate, suggesting that both SCFA transport and metabolism are critical for HCO3secretion. Both lumen and serosal 25 mM butyrate inhibit cAMP-induced HCO3secretion to a comparable degree (98 vs. 90%). In contrast, Cl-dependent HCO3secretion is downregulated by lumen 25 mM butyrate considerably more than by serosal butyrate (98 vs. 37%). Butyrate did not induce HCO3secretion in isolated microperfused crypts, whereas an outward-directed HCO3gradient-driven induced14C-butyrate uptake by surface but not crypt cell AMV. Both36Cl/HCO3exchange and potential-dependent36Cl movement in AMV were inhibited by 96–98% by 20 mM butyrate. We conclude that 1) SCFA-dependent HCO3secretion is the result of SCFA transport across the apical membrane via a SCFA/HCO3exchange more than intracellular SCFA metabolism; 2) SCFA-dependent HCO3secretion is most likely a result of an apical membrane SCFA/HCO3exchange in surface epithelial cells; 3) SCFA downregulates Cl-dependent and cAMP-induced HCO3secretion secondary to SCFA inhibition of apical membrane Cl/HCO3exchange and anion channel activity, respectively.
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Chu, Shuhan, Hongxu Zhang, and Lixin Ding. "Simultaneous Determination of Three Effective Components in Different Compatibility Proportion of the Couple of Sophora flavescens-Fructus Ligustri lucidi by HPLC." MATEC Web of Conferences 242 (2018): 01015. http://dx.doi.org/10.1051/matecconf/201824201015.
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Sophora flavescens - Ligustrum lucidum has the effect of increasing the anti-cancer effect of white blood cells and treating liver fibrosis. In order to determine the optimal compatibility ratio of this drug pair, the rationality of the drug pairs in different proportions was revealed. In this experiment, three active ingredients of the Sophora flavescens-Fructus Ligustri lucidi were extracted by reflux extraction, which were matrine, oxymatrine and specnuezhenide. A method for simultaneously determining the content of matrine, oxymatrine and specnuezhenide in a short time by HPLC was established. The method is simple, precise, and stable. Reproducibility can be used to determine the content of three active ingredients of matrine, oxymatrineand specnuezhenide. The analysis results was indicated that the different proportions of compatibility and different extraction solvents have significant effects on the content of active ingredients and dissolution rate of Sophora flavescens- Fructus Ligustri lucidi, after the combination of Sophora flavescens- Fructus Ligustri lucidi in different proportions, the content of matrine, oxymatrine and specnuezhenide is greater than the content of the active ingredients of each single herb. When the ratio of compatibility of Sophora flavescens-Ligustrum Ligustri lucidi is <1:1, it is beneficial to the dissolution of the three active ingredients. By determining the optimal compatibility ratio of this drug pair, provided a theoretical basis for the study of late pharmacodynamics and provide theoretical basis for clinicalapplication of traditional Chinese medicine.
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Quílez Esteve, Laia. "Hacia una teoría de la posmemoria. Reflexiones en torno a las representaciones de la memoria generacional / Towards a Theory of Postmemory. Reflections on the Representations of Generational Memory." Historiografías, no. 8 (December 28, 2017): 57. http://dx.doi.org/10.26754/ojs_historiografias/hrht.201482417.
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This article attempts to address, from the theoretical field, the different approaches to “postmemory” that have emerged in Western culture since the 1990s. After mapping the genealogical origins of this term, paying attention to its transformations and to discussions that this category has prompted, we shall examine the formal issues that govern most of this kind of cultural productions. To this end we expose three examples that have been produced in very different artistic fields, periods, geographies, and traumatic pasts: Maus, a graphic novel by Art Spiegelman; Arqueología de la ausencia, a photographic project by the Argentinian Lucila Quieto; and Haciendo memoria, a short documentary by the Spanish Sandra Ruesga.Key WordsPostmemory, generational memory, memory mediatisation, traumatic past, audiovisual culture, contemporary narratives.ResumenEl presente artículo se propone abordar desde el ámbito teórico las distintas aproximaciones que desde los años noventa del pasado siglo se han hecho al concepto de la “posmemoria”. Tras este repaso genealógico por los orígenes, transformaciones y debates que ha suscitado dicha categoría, se delinean las cuestiones formales que rigen la mayor parte las producciones culturales inspiradas por la posmemoria. Para tal fin se han seleccionado tres ejemplos que abarcan ámbitos artísticos, épocas, geografías y pasados traumáticos muy diversos: Maus, la novela gráfica de Art Spiegelman; Arqueología de la ausencia, el proyecto fotográfico de la argentina Lucila Quieto; y Haciendo memoria, un cortometraje documental de la española Sandra RuesgaPalabras clavePosmemoria, memoria generacional, mediatización de la memoria, cultura audiovisual, pasado traumático, narrativa contemporánea.
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Kang, Lu, Keyuan Zheng, Yuqing Xie, Yanwen Deng, Yina Yu, Mulan Zhu, Ruchun Xi, and Xiaomei Deng. "Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants." Plants 9, no. 8 (August 5, 2020): 997. http://dx.doi.org/10.3390/plants9080997.
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Magnolia lucida (Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype of M. lucida as the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth of M. lucida. Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting in M. lucida plants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant.
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Hong, Ying, and Zeng Min Geng. "Research and Realization of a Search Engine System for Professional Field." Advanced Materials Research 850-851 (December 2013): 745–50. http://dx.doi.org/10.4028/www.scientific.net/amr.850-851.745.
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In the light of the deficiency of general search engine technology in professional retrieval,This paper researched and designed a search engine system for professional field (SESPF for short).This system automatically crawls web pages by the spider program.It introduced professional dictionary and filtered the webpages information according to certain rules.At the same time,the system improved the PageRank algorithm and Lucene webpage ranking algorithm.The experimental results show that this system has a higher precision in professional field retrieval compared with the general search engine.
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Alonso, Mariângela. "A especularidade na produção jornalística de Clarice Lispector." Journal of Lusophone Studies 4, no. 2 (January 1, 2020): 97–116. http://dx.doi.org/10.21471/jls.v4i2.337.
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This article aims to understand the narrative technique of mise-enabyme in Clarice Lispector’s short story “A quinta história” (published in A legião estrangeira (1964)). Here Lispector quickly builds variations on the same argument, a sort of unfolding of stories that succeed each other, originating in the same idea: how to kill cockroaches. Based on the studies of Lucien Dällenbach and Verónique Labeille among others, the article confronts the versions of A quinta história in the periodicals Casa e Jardim and Senhor, focusing on mise-enabyme as the seminal and questioning force of Lispector’s work.
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SETYAYUDI, ALI, KRISNAWATI KRISNAWATI, and RYKE NANDINI. "Short Communication: Exploration of bidara laut (Strychnos lucida) parent trees in Gunung Tunak Ecotourism Park, West Nusa Tenggara, Indonesia." Biodiversitas Journal of Biological Diversity 20, no. 1 (December 4, 2018): 274–78. http://dx.doi.org/10.13057/biodiv/d200144.
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Setyayudi A, Krisnawati, Nandini R. 2019. Short Communication: Exploration of bidara laut (Strychnos lucida) parent trees in Gunung Tunak Ecotourism Park, West Nusa Tenggara, Indonesia. Biodiversitas 20: 274-278. Bidara laut (Strychnos lucida R. Brown) is one of the widely used medicinal plants. There is a bidara laut wood processing industry in West Nusa Tenggara that produces drinking cups called ‘health cups’ utilizing bidara laut. All raw materials used for the production of health cups are, however, obtained from bidara laut stands in forest areas, which may cause the extinction of this medicinal plant species if the highly intensive raw material harvesting in the forest is not supported with cultivation of this plant species. To preserve this plant species, the Institute for Research and Development of Non-Timber Forest Products Technology (NTFP-RDI) has carried out breeding activities by developing a collection garden of this species from the populations of Bali and West Nusa Tenggara (Lombok and Sumbawa Islands). The initial step carried out was the exploration of the parent trees followed by collection of generative materials (seeds) and nurseries. Exploration of bidara laut parent trees on Lombok Island was carried out in Gunung Tunak Ecotourism Park using a purposive sampling method, followed by collection of fruits to obtain seed propagating materials for establishment of seedling nurseries. Twenty-five parent trees were identified with average height of nine m, stem diameter of 24 cm and stem straightness scales of 1-3. The average seed produced was 413 seeds tree-1. Three parent trees were selected based on the highest seed germination rates, namely parent trees number 6, 14 and 20. This result provides useful information for the next bidara laut breeding activities.
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Schittny, J. C., R. Timpl, and J. Engel. "High resolution immunoelectron microscopic localization of functional domains of laminin, nidogen, and heparan sulfate proteoglycan in epithelial basement membrane of mouse cornea reveals different topological orientations." Journal of Cell Biology 107, no. 4 (October 1, 1988): 1599–610. http://dx.doi.org/10.1083/jcb.107.4.1599.
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Thin and ultrathin cryosections of mouse cornea were labeled with affinity-purified antibodies directed against either laminin, its central segments (domain 1), the end of its long arm (domain 3), the end of one of its short arms (domain 4), nidogen, or low density heparan sulfate proteoglycan. All basement membrane proteins are detected by indirect immunofluorescence exclusively in the epithelial basement membrane, in Descemet's membrane, and in small amorphous plaques located in the stroma. Immunoelectron microscopy using the protein A-gold technique demonstrated laminin domain 1 and nidogen in a narrow segment of the lamina densa at the junction to the lamina lucida within the epithelial basement membrane. Domain 3 shows three preferred locations at both the cellular and stromal boundaries of the epithelial basement membrane and in its center. Domain 4 is located predominantly in the lamina lucida and the adjacent half of the lamina densa. The low density heparan sulfate proteoglycan is found all across the basement membrane showing a similar uniform distribution as with antibodies against the whole laminin molecule. In Descemet's membrane an even distribution was found with all these antibodies. It is concluded that within the epithelial basement membrane the center of the laminin molecule is located near the lamina densa/lamina lucida junction and that its long arm favors three major orientations. One is close to the cell surface indicating binding to a cell receptor, while the other two are directed to internal matrix structures. The apparent codistribution of laminin domain 1 and nidogen agrees with biochemical evidence that nidogen binds to this domain.
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Grecu, Valentin, and Călin Deneş. "Teaching Sustainability Through Movie Making Activities." Balkan Region Conference on Engineering and Business Education 1, no. 1 (August 15, 2014): 367–72. http://dx.doi.org/10.2478/cplbu-2014-0058.
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AbstractThe role of education in shaping the future is widely recognized. The world is becoming more complex, interdependent and unsustainable and this calls for a change in lifestyle. Thus, education for sustainable development is given increased attention in universities worldwide. Transformation of education into sustainability education implies systemic thinking and interdisciplinary approaches. At “Lucian Blaga” University of Sibiu there has been carried out an experimental, optional course which aimed to achieve an integrated approach to sustainability, fostering dialogue across multiple areas of knowledge. This paper presents an analysis of the activity of creating short video-clips related to sustainability and the impact that this teaching method has on the students.
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Janzsó, Miklós. "„Vivere omnes beate volunt...”." Belvedere Meridionale 31, no. 1 (2019): 80–88. http://dx.doi.org/10.14232/belv.2019.1.5.
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In this paper I tried to demonstrate parallels between the works of Seneca the Younger, and the so-called Tabula Cebetis. This short dialogue from the first century AD contains moral teachings about reaching true happiness. Contemporary authors like Lucian, Dio Chrysostom and Plutarch are the closest parallels in using allegories and phrases, but the ethical teaching of Seneca is the most comparable with the whole teaching of the Tabula Cebetis. I tried to highlight some phrases which can prove the similarity of the thoughts of both authors. The works of Seneca may also provide a clue to reveal the author of this anonymous work.
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Chen, Beibei, Junping Wei, Ruyuan Zhu, Hao Zhang, Bingke Xia, Yage Liu, Xuan Dai, et al. "Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats." Journal of Ethnopharmacology 279 (October 2021): 114348. http://dx.doi.org/10.1016/j.jep.2021.114348.
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Bergen-Aurand, Brian. "Screened Women." Screen Bodies 1, no. 2 (December 1, 2016): 1–3. http://dx.doi.org/10.3167/screen.2016.010201.
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As this issue of Screen Bodies went into production, the 2016 Democratic National Convention in Philadelphia, Pennsylvania, was concluding, and Vogue published a short online piece on Hillary Clinton’s sartorial choices for her acceptance speech. While Clinton had often worn brighter, bolder colors and styles for her major appearances, on this evening she donned an ivory suit—a simple canvas against which to continue painting an image of the woman behind the candidacy. At the same time, the Internet continued to disseminate more negative reviews of the rebooted Ghostbusters (2016)—starring Melissa McCarthy, Kristen Wiig, Kate McKinnon, and Leslie Jones, directed by Paul Feig, written by Katie Dippold and Paul Feig. I think the film is (pace Lucille Ball) funny and brave. It hilariously reverses many sexist tropes and assumptions out of mainstream Hollywood films; it is crude and inappropriate in the best of ways; and responses to it—especially patriarchal, masculinist, heteronormative, and racist ones—continue to demonstrate the importance of critical comedic revisions for feminist interruptions of established paradigms.
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Suzuki, Takuya, Shoko Yoshida, and Hiroshi Hara. "Physiological concentrations of short-chain fatty acids immediately suppress colonic epithelial permeability." British Journal of Nutrition 100, no. 2 (August 2008): 297–305. http://dx.doi.org/10.1017/s0007114508888733.
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Colonic fermentation products, SCFA, have various effects on colonic functions. Here, we found that physiological concentrations of SCFA immediately promote epithelial barrier function in the large intestine. Solutions of mixed and individual SCFA were applied to the caecal walls mounted on Ussing-type chambers. Transepithelial electrical resistance (TER) increased rapidly and reached a peak 35 % higher than that in the control specimen within 10 min post application of the SCFA mixture (80 acetate, 40 propionate, 20 butyrate (mmol/l)). The Lucifer yellow permeability, a paracellular transport marker, was dose-dependently reduced by the mixed SCFA, acetate and propionate solutions. Inhibition of monocarboxylate transporter-1 did not influence the increase in TER with acetate; however, lowering the pH (from 7·5 to 5·5) clearly enhanced the effect of acetate. Non-metabolizable, bromo and chloro derivatives of SCFA also increased TER. These results suggest that passive diffusion of SCFA is dominant and the metabolism of SCFA is not required for the promotive effect of SCFA on barrier function. We also observed that individual SCFA dose-dependently increased TER in T84 and Caco-2 cells, which indicates that SCFA directly stimulate epithelial cells. Depletion of membrane cholesterol and inhibitors of phosphatidylinositol-3 kinase and Gq protein attenuated the acetate-mediated promotive effect. Finally, we found that the mucosal application of the SCFA mixture dose-dependently suppressed [3H] mannitol transport from the caecal lumen to the mesenteric blood in the anaesthetized rats. We conclude that physiological concentrations of SCFA immediately enhance barrier function of the colonic epithelium through cholesterol-rich microdomain in the plasma membrane.
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Shen, Weiwei, Bin Wang, Jian Pu, and Jun Wang. "The Kelly Growth Optimal Portfolio with Ensemble Learning." Proceedings of the AAAI Conference on Artificial Intelligence 33 (July 17, 2019): 1134–41. http://dx.doi.org/10.1609/aaai.v33i01.33011134.
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As a competitive alternative to the Markowitz mean-variance portfolio, the Kelly growth optimal portfolio has drawn sufficient attention in investment science. While the growth optimal portfolio is theoretically guaranteed to dominate any other portfolio with probability 1 in the long run, it practically tends to be highly risky in the short term. Moreover, empirical analysis and performance enhancement studies under practical settings are surprisingly short. In particular, how to handle the challenging but realistic condition with insufficient training data has barely been investigated. In order to fill voids, especially grappling with the difficulty from small samples, in this paper, we propose a growth optimal portfolio strategy equipped with ensemble learning. We synergically leverage the bootstrap aggregating algorithm and the random subspace method into portfolio construction to mitigate estimation error. We analyze the behavior and hyperparameter selection of the proposed strategy by simulation, and then corroborate its effectiveness by comparing its out-of-sample performance with those of 10 competing strategies on four datasets. Experimental results lucidly confirm that the new strategy has superiority in extensive evaluation criteria.
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Lowy, R. J., D. C. Dawson, and S. A. Ernst. "Primary culture of duck salt gland. II. Neurohormonal stimulation of active transport." American Journal of Physiology-Cell Physiology 249, no. 1 (July 1, 1985): C41—C47. http://dx.doi.org/10.1152/ajpcell.1985.249.1.c41.
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Primary cultures of structurally polarized sheets of avian salt gland secretory cells were mounted in Lucite chambers for transmural electrophysiological analysis. Transmural resistance values increased during the first 3 days of culture to 293 +/- 35 omega X cm2 and then decreased slowly thereafter. There was little short-circuit current (Isc) in the absence of secretagogues. Serosal addition of either carbachol or epinephrine resulted in a Isc consistent with positive charge flow from mucosa to serosa, thus demonstrating that these cell layers were capable of active ion transport in response to either cholinergic or adrenergic neurohormonal stimulation. Serosal ouabain or furosemide abolished the response to either agonist, while theophylline enhanced the response. Receptor specificity for the electrical responses was shown by selective inhibition of carbachol- and epinephrine-induced Isc by atropine and propranolol, respectively. The results demonstrate that these primary epithelial cell cultures are capable of active ion transport and are sensitive to known inhibitors of secretory transport, and suggest that intracellular coupling mechanisms for hormonal control are retained in culture. These cultures should be useful for studying mechanisms of ion secretory transport and their regulatory control.
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Kabiling, Michael B., Michael E. Trudnak, and Richard Bouchard. "EVALUATION OF SHORELINE STABILIZATION USING A HYBRID SHORELINE MORPHOLOGY MODEL." Coastal Engineering Proceedings, no. 36 (December 30, 2018): 50. http://dx.doi.org/10.9753/icce.v36.sediment.50.
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The Fort Pierce Shore Protection Project, located in St. Lucie County, Florida, nourishes a 2.09-km Atlantic Ocean shoreline south of Fort Pierce Inlet on Florida’s east coast. After each beach nourishment, historical measurements indicate the beach fill erodes nonuniformly, with a hotspot along the northernmost 0.7 km requiring nourishment after about two years of normal wave regimes. However, storms can quickly erode the beach fill and require nourishment earlier than the normal two-year interval. Longshore transport carries most of the eroded fill to the south, creating a strong feeder-beach effect. This study evaluated several designs and combinations of coastal structures to produce more uniform erosion throughout the project area and to increase the nourishment interval. The study applied several state-of-the-art numerical models to evaluate the two-dimensional effect of a “coastal structures†alternative on hydrodynamics, waves, sediment transport, and beach morphology.
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Rosenbaum, Yudith. "Entre a loucura e a lucidez: crônicas de Clarice Lispector no Jornal do Brasil." Journal of Lusophone Studies 4, no. 2 (January 1, 2020): 75–96. http://dx.doi.org/10.21471/jls.v4i2.336.
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This article approaches the territory of madness and unreason in Lispector’s fiction, an aspect of her work that is present in her press contributions “Das doçuras de Deus” (16 Dec. 1967), “Sem nosso sentido humano” (28 Jun. 1969), “Loucura diferente” (8 Dec. 1970), and “Lucidez perigosa” (2 May 1972). None of these texts became an autonomous short story or novel, as happened with many others originally published in Jornal do Brasil between 1967 and 1973. This study examines these four texts with a view to grasping a Claricean poetics, as it is represented through extreme perceptions of madness and unreason. I consider these texts as pre-figurations or instances of contact with motives such as freedom, adaptation, fear and habit. The texts examined here are shown to defy hegemonic rationality while pointing to the limits of discursive language.
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Buzdugan, Adrian. "The Cioran case – a heideggerian case?" Sæculum 48, no. 2 (December 1, 2019): 22–28. http://dx.doi.org/10.2478/saec-2019-0026.
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AbstractIf Cioran’s articles could be easily examined in terms of their political message, his aforisms, short texts, or essays have nothing to justify their analysis from the perspective of their “political” content. Cioran’s thoughts, bordering on the poetic, are not comparable to Heidegger’s writings in which, as some try to convince us, the totalitarian ideology has deeply penetrated the very core of the ontology he developed. Even if scholars have identified features of national-socialism in Heidegger’s works, it is still difficult to blame him for his ideas since he has quickly and lucidly reconsidered his approach to the Nazi ideology. Cioran’s writings have nothing in common with Corneliu Zelea Codreanu’s books, For My Legionaries or The Nest Leader’s Manual. Corneliu Zelea Codreanu’s hatred for Judeo-Communism has no correspondent in Emil Cioran’s writings.
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Oprean, Constantin, Mihaela Alina Vanu, and Silvia Florea. "An Analysis Of New Graduate-Tracing Metrics Of University Management Performance At The Lucian Blaga University Of Sibiu (Romania)." Balkan Region Conference on Engineering and Business Education 1, no. 1 (August 15, 2014): 513–16. http://dx.doi.org/10.2478/cplbu-2014-0088.
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AbstractLucian Blaga University of Sibiu, Romania is moving rapidly towards more evidence-based accountability and, in this context, it has a growing concern with an ever more adequate provision for labor market demands in the region. The present paper analyzes the new graduate-tracing metrics of university management performance and goals between 2004 and 2012 and sets the short-term goals agenda for enhancement of graduate employment and employability at LBUS.
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Carter, J. P., H. G. Poulos, and R. I. Tanner. "John Robert Booker 19421998." Historical Records of Australian Science 14, no. 2 (2002): 143. http://dx.doi.org/10.1071/hr02008.
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Professor John Robert Booker died in Concord Hospital in Sydney on 13 January 1998, after a long and courageously-fought battle against cancer. His death cut short a brilliant academic career and deprived the Australian geotechnical and engineering mechanics communities of one of its most eminent members. At the time of his death John Booker held a personal chair in engineering mechanics in the Department of Civil Engineering at the University of Sydney, and he was widely regarded as one of the finest researchers of his generation working in the field of theoretical geomechanics. His long battle with cancer did not deflect him from his life's work. While understandably, he was unable to hold formal classes during the last months of his life, it is significant that he was active in research until his very last weeks, such was his love for and dedication to his work. John Booker was a warm, friendly, caring man who touched many lives. He was mentor to most with whom he came into close contact, students and colleagues alike. He is survived by his second wife Elizabeth, daughters from his first marriage, Katie and Lucie, sister Judith and mother Joan.
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Ermentrout, B., Jing W. Wang, Jorge Flores, and A. Gelperin. "Model for Olfactory Discrimination and Learning inLimax Procerebrum Incorporating Oscillatory Dynamics and Wave Propagation." Journal of Neurophysiology 85, no. 4 (April 1, 2001): 1444–52. http://dx.doi.org/10.1152/jn.2001.85.4.1444.
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We extend our model of the procerebral (PC) lobe of Limax, which is comprised of a layer of coupled oscillators and a layer of memory neurons, each layer 4 rows by 20 columns, corresponding to the cell body layer (burster cells) and neuropil layer (nonburster cells) of the PC lobe. A gradient of connections in the layer of model burster cells induces periodic wave propagation, as measured in the PC lobe. We study odor representations in the biological PC lobe using the technique of Kimura and coworkers. Lucifer yellow injection into intact Limax after appetitive or aversive odor learning results in a band or patch of labeled cells in the PC lobe with the band long axis normal to the axis of wave propagation. Learning two odors yields two parallel bands of labeled PC cells. We introduce olfactory input to our model PC lobe such that each odor maximally activates a unique row of four cells which produces a short-term memory trace of odor stimulation. A winner-take-all synaptic competition enabled by collapse of the phase gradient during odor presentation produces a single short-term memory band for each odor. The short-term memory is converted to long-term memory if odor stimulation is followed by activation of an input pathway for the unconditioned stimulus (US) which presumably results in release of one or more neuromodulatory amines or peptides in the PC lobe.
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BROADIE, MARK, and WEIWEI SHEN. "HIGH-DIMENSIONAL PORTFOLIO OPTIMIZATION WITH TRANSACTION COSTS." International Journal of Theoretical and Applied Finance 19, no. 04 (May 25, 2016): 1650025. http://dx.doi.org/10.1142/s0219024916500254.
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This paper studies Merton’s portfolio optimization problem with proportional transaction costs in a discrete-time finite horizon. Facing short-sale and borrowing constraints, investors have access to a risk-free asset and multiple risky assets whose returns follow a multivariate geometric Brownian motion. Lower and upper bounds for optimal solutions up to the problem with 20 risky assets and 40 investment periods are computed. Three lower bounds are proposed: the value function optimization (VF), the hyper-sphere and the hyper-cube policy parameterizations (HS and HC). VF attacks the conundrums in traditional value function iteration for high-dimensional dynamic programs with continuous decision and state spaces. HS and HC respectively approximate the geometry of the trading policy in the high-dimensional state space by two surfaces. To evaluate lower bounds, two new upper bounds are provided via a duality method based on a new auxiliary problem (OMG and OMG2). Compared with existing methods across various suites of parameters, new methods lucidly show superiority. The three lower bound methods always achieve higher utilities, HS and HC cut run times by a factor of 100, and OMG and OMG2 mostly provide tighter upper bounds. In addition, how the no-trading region characterizing the optimal policy deforms when short-sale and borrowing constraints bind is investigated.
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Kabiling, Michael B. "A 3-D MODEL USING REPRESENTATIVE WAVES METHOD FOR SHORELINE MORPHODYNAMICS AROUND COASTAL STRUCTURES." Coastal Engineering Proceedings, no. 36v (December 28, 2020): 28. http://dx.doi.org/10.9753/icce.v36v.sediment.28.
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The Fort Pierce Shore Protection Project nourishes a 2.1-km Atlantic Ocean shoreline south of Fort Pierce Inlet in St. Lucie County, Florida. The beach fill erodes nonuniformly with a hotspot along the northernmost 0.7 km requiring nourishment after about two years of normal wave regime. This study validated and applied to beach stabilization a shoreline morphology model to evaluate designs and combinations of coastal structures to increase the nourishment interval. Simulations of long-term normal tides, waves, and storm conditions show (a) shoreline movement pattern similar to the general historical pattern observed in the project area, including accurately indicating the largest erosion rate and shoreline retreat along the first 0.7 km south of the jetty; (b) the coastal structures retain beach fill longer, resulting in lower erosion rates from 0.8 to 6.4 km south of the jetty; and (c) the coastal structures alternative extends the normal beach nourishment interval from the current two years to four years. Application of representative waves allows accurate and efficient modeling of long-term 3-D beach and shoreline deformation.
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Coletes-Blanco, Agustín. "A Forgotten ‘Romantic’ Excursion: Joseph Blanco White's A Journey to the Trosacks in 1816." Romanticism 27, no. 2 (July 2021): 214–25. http://dx.doi.org/10.3366/rom.2021.0510.
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In 1816 Joseph Blanco White visited the Trossachs, having travelled to Edinburgh as a member of the household of Lord and Lady Holland. Soon afterwards he wrote A Journey to the Trosacks in 1816, a short but fascinating account of his trip which has remained unpublished until now. Lucidly penned, this autograph text shows admiration for the Scottish wilderness and interest in technological feats such as the steamboat that he takes on the Firth of Forth, an absolute novelty at the time. Observations on Highlands customs and language, and literary allusions to Sterne, Scott, Johnson and Boswell add to the interest of this forgotten piece, as do remarks about John Murray the publisher and Dugald Stewart the philosopher. The aim of this article is to present for the first time this work as a document of literary and cultural importance, given the renewed interest of Romantic era scholarship in travel writing and in Blanco White, the most important Spanish cultural mediator in Britain during the first decades of the 19th century.
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Shibli, Rida, L. Art Spomer, and Mary Ann Lila Smith. "OSMOTIC ADJUSTMENT AND GROWTH RESPONSE OF THREE in vitro GROWN Chrysanthemum morifolium Ramat. CULTIVARS TO OSMOTIC STRESS." HortScience 25, no. 9 (September 1990): 1125a—1125. http://dx.doi.org/10.21273/hortsci.25.9.1125a.
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Osmotic adjustment in response to decreasing media water availability was observed for in vitro Chrysanthemum morifolium Ramat. cultivars Bright Golden Anne, Deep Luv, and Lucido. Water stress was induced by increasing sorbitol (0, 0.1, 0.2, 0.3, 0.4 M), mannitol (0, 0.1, 0.2, 0.3, 0.4 M), and sucrose (30, 45, 60, 75, 90 g·l-1) concentrations in modified MS media (2 mg·l-1 BA and 0.1 mg·l-1 NAA). Osmotic adjustment was evidenced by a significant reduction in measured cell sap osmotic potential (R2 = 0.78, 0.96, 0.91 for sucrose, sorbitol, and mannitol respectively) in all cultivars. Shoot length, weighted density (apparent mass), and proliferation were significantly reduced by sorbitol and mannitol treatments. Sucrose reduced shoot proliferation, increased length, and had an inconsistent effect on weighted density. Cultures grown on media without hormones showed tremendous increase in root number up to 60 g·l-1 sucrose. Sorbitol had a negligible effect on rooting at 0.1 M but no roots developed at higher sorbitol concentrations or in any mannitol treatments. Plants transferred to a non-water-stress media after they had experienced in vitro water stress exhibited no change in osmotic properties from the stress treatments.
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Guan, Yanfang, Roger T. Worrell, Timothy A. Pritts, and Marshall H. Montrose. "Intestinal ischemia-reperfusion injury: reversible and irreversible damage imaged in vivo." American Journal of Physiology-Gastrointestinal and Liver Physiology 297, no. 1 (July 2009): G187—G196. http://dx.doi.org/10.1152/ajpgi.90595.2008.
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The early events in an intestinal ischemic episode have been difficult to evaluate. Using in vivo microscopy we have analyzed in real-time the effects of short (15 min) and long (40–50 min) ischemia with subsequent reperfusion (IR), evaluating structure, integrity, and functioning of the mouse jejunal mucosa while monitoring blood flow by confocal microscopy. IR was imposed by inflation/deflation of a vascular occluder, and blood flow was monitored and confirmed with scanning confocal imaging. After short ischemia, villus tip cells revealed a rapid increase (23%) in the intracellular NAD(P)H concentration (confocal autofluorescence microscopy), and the pH-sensitive probe BCECF showed a biphasic response of the intracellular pH (pHi), quickly alkalinizing from the resting value of 6.8 ± 0.1 to 7.1 ± 0.1 but then strongly acidifying to 6.3 ± 0.1. Upon reperfusion, values returned toward control. In contrast, results were heterogeneous after long IR. During long ischemia, one-third of the epithelial cells remained viable with reversible changes upon reperfusion, but remaining cells lost membrane integrity (Lucifer Yellow uptake, LY) and had membrane blebs during ischemia. These effects became more pronounced as the reperfusion interval progressed when cells exhibited more severely affected NAD(P)H and pHi values, larger blebs, and more LY uptake and eventually were shed from the villus. Results from stereo microscopy suggest that these irreversible effects of IR may have occurred as a result of incomplete restorations of local blood flow, especially at the antimesenteric side of the intestine. We conclude that the adverse effects of short ischemia on the jejunum epithelium are fully reversible during the reperfusion interval. However, after long ischemia, reperfusion cannot restore normal structure and functioning of a majority of cells, which deteriorate further. Our results provide a basis for defining the cellular events that cause tissue to transit from reversible to irreversible damage during IR.
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Hoque, Kazi Mirajul, Vazhaikkurichi M. Rajendran, and Henry J. Binder. "Zinc inhibits cAMP-stimulated Cl secretion via basolateral K-channel blockade in rat ileum." American Journal of Physiology-Gastrointestinal and Liver Physiology 288, no. 5 (May 2005): G956—G963. http://dx.doi.org/10.1152/ajpgi.00441.2004.
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Zn, an essential micronutrient and second most abundant trace element in cell and tissues, reduces stool output when administered to children with acute diarrhea. The mechanism by which Zn improves diarrhea is not known but could result from stimulating Na absorption and/or inhibiting anion secretion. The aim of this study was to investigate the direct effect of Zn on intestinal epithelial ion absorption and secretion. Rat ileum was partially stripped of serosal and muscle layers, and the mucosa was mounted in lucite chambers. Potential difference and short-circuit current were measured by conventional current-voltage clamp method.86Rb efflux and uptake were assessed for serosal K channel and Na-K-2Cl cotransport activity, respectively. Efflux experiments were performed in isolated cells preloaded with86Rb in the presence of ouabain and bumetanide, whereas uptake experiments were performed in low-Cl isotonic buffer containing Ba and ouabain. Neither mucosal nor serosal Zn affected glucose-stimulated Na absorption. In contrast, forskolin-induced Cl secretion was markedly reduced by serosal but not mucosal addition of Zn. Zn also substantially reversed the increase in Cl secretion induced by 8-bromoadenosine 3′,5′-cyclic monophosphate (8-BrcAMP) with half-maximal inhibitory concentration of 0.43 mM. In contrast, serosal Zn did not alter Cl secretion stimulated by carbachol, a Ca-dependent agonist. Zn inhibited 8-BrcAMP-stimulated86Rb efflux but not carbachol-stimulated86Rb efflux. Zn had no effect on bumetanide-sensitive86Rb uptake, Na-K-ATPase, or CFTR. We conclude from these studies that Zn inhibits cAMP-induced Cl secretion by blocking basolateral membrane K channels.
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Nasako, Haruka, Yui Takashina, Hiroaki Eguchi, Ayaka Ito, Yoshinobu Ishikawa, Toshiyuki Matsunaga, Satoshi Endo, and Akira Ikari. "Increase in Toxicity of Anticancer Drugs by PMTPV, a Claudin-1-Binding Peptide, Mediated via Down-Regulation of Claudin-1 in Human Lung Adenocarcinoma A549 Cells." International Journal of Molecular Sciences 21, no. 16 (August 17, 2020): 5909. http://dx.doi.org/10.3390/ijms21165909.
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Claudin-1 (CLDN1), a tight junctional protein, is highly expressed in lung cancer cells and may contribute to chemoresistance. A drug which decreases CLDN1 expression could be a chemosensitizer for enhancing the efficacy of anticancer drugs, but there is no such drug known. We found that PMTPV, a short peptide, which mimics the structure of second extracellular loop (ECL2) of CLDN1, can reduce the protein level of CLDN1 without affecting the mRNA level in A549 cells derived from human lung adenocarcinoma. The PMTPV-induced decrease in CLDN1 expression was inhibited by monodansylcadaverine, a clathrin-mediated endocytosis inhibitor, and chloroquine, a lysosome inhibitor. Quartz crystal microbalance assay showed that PMTPV can directly bind to the ECL2 of CLDN1. In transwell assay, PMTPV increased fluxes of Lucifer yellow (LY), a paracellular flux marker, and doxorubicin (DXR), an anthracycline anticancer drug, without affecting transepithelial electrical resistance. In three-dimensional spheroid culture, the size and cell viability were unchanged by short peptides, but the fluorescence intensity of hypoxia probe LOX-1 was decreased by PMTPV. PMTPV elevated the accumulation and cytotoxicity of DXR in the spheroids. Similar results were observed by knockdown of CLDN1. Furthermore, the sensitivities to cisplatin (CDDP), docetaxel, and gefitinib were enhanced by PMTPV. The level of CLDN1 expression in CDDP-resistant cells was higher than that in parental A549 cells, which was reduced by PMTPV. PMTPV restored the toxicity to DXR in the CDDP-resistant cells. Our data suggest that PMTPV may become a novel chemosensitizer for lung adenocarcinoma.
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Lucotte, Gerard, and Peter Hrechdakian. "New Advances Reconstructing the Y Chromosome Haplotype of Napoleon the First Based on Three of his Living Descendants." Journal of Molecular Biology Research 5, no. 1 (December 19, 2014): 1. http://dx.doi.org/10.5539/jmbr.v5n1p1.
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This paper describes the findings of the complete reconstruction of the lineage Y chromosome haplotype of the French Emperor Napoléon I. In a previous study (Lucotte et al., 2013) we reconstructed, for more than one hundred Y-STRs (Y–short tandem repeats), the Y-chromosome haplotype of Napoléon I based on data comparing STR allelic values obtained from the DNA of two of his living descendants: Charles Napoléon (C.N.) and Alexandre Colonna Walewski (A.C.W.); in the present study we compare STR allelic values of C.N. and A.C.W. to those of Mike Clovis (M.C.), a living fifth generation descendant of Lucien (one of Napoléon’s brothers). When compared between M.C., C.N. and A.C.W., STR allelic values are identical for a total of 93 STRs; that permits us to propose those values, for which the three living descendants are identical, as expected allelic values of Napoléon I’s Y-chromosome haplotype. For seven STRs, allele values are variable between M.C., C.N. and A.C.W.; we propose for three of them (DYS442, DYS454 and DYS712) expected allelic values, based on data concerning the allele distributions of these STRs in the population.
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Ebrahimi, Maryam, Soheila Rouhani, Iraj Mobedi, Ali Rostami, Hoshang Khazan, and Mohammad Bagher Ahoo. "Prevalence and Morphological Characterization ofCheilospirura hamulosa, Diesing, 1861 (Nematoda: Acuarioidea), from Partridges in Iran." Journal of Parasitology Research 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/569340.
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This study reports data on the prevalence, morphology, and morphometry of the nematodeCheilospirura hamulosaon the basis of light and stereoscopic microscopy and also camera lucida. Specimens were recovered after necropsies of 100 partridges (Alectoris chukar) from Taleqan County in Alborz Province, Iran. The prevalence ofC. hamulosain partridges was of 30% with a mean intensity of 3.9 and range of infection of 1–12. The mean length and width of females were17.5±2.14and0.39±0.04 mm, while those of males were12.2±0.67and0.3±0.06 mm, respectively. The characteristic digitiform tail was observed in females, and the unequal spicules, caudal alae, and ten pairs of caudal papillae were seen in males. The taxonomic characteristic longitudinal cordons and muscular and glandular oesophagus were observed in both sexes. Ratio between cordons and body length in males and females was 1 : 1.33 and 1 : 1.68, respectively. Ratio between long and short spicules in males was 1 : 2.3. The average size of embryonated eggs was51.25×29.5 μm. In the present study,C. hamulosa(Nematoda: Acuarioidea) is recorded for the first time from partridges in Iran. Therefore, the morphological characters described in this study will be useful in the future diagnostic and taxonomic studies of Acuarioidea family.
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Whitmarsh, Tim. "Greek and Roman in dialogue: the pseudo-Lucianic Nero." Journal of Hellenic Studies 119 (November 1999): 142–60. http://dx.doi.org/10.2307/632315.
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The short dialogue entitled Nero or on the digging of the Isthmus, preserved in the manuscripts of Lucian, is an intriguing piece. The contents are quickly summarised. Nero's abandoned attempt to dig through the Isthmus of Corinth is discussed by the two interlocutors, a certain Menecrates and the philosopher Musonius Rufus, who is said to have taken part in the digging (1). The scene is apparently the rugged Aegean island of Gyara to which the historical Musonius was exiled. The discussion broadens out to include Nero's tour of Greece, with a particular focus upon his singing; and it concludes as the news breaks of Nero's death (11). Menecrates' role in the discussion is limited to that of ‘prompter’, while Musonius assumes the authoritative, pedagogic role in the dialogue. Is there any unified meaning to this text? And why the dialogue form (given that Menecrates' role in it is so perfunctory)? This paper proposes one set of answers to these questions, by siting the Nero in the context of the cultural history of Greco-Roman relations, an area that has attracted much attention over the years (and has been further reinvigorated in the light of post-colonial theory).
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Chu, S., W. E. Brownell, and M. H. Montrose. "Quantitative confocal imaging along the crypt-to-surface axis of colonic crypts." American Journal of Physiology-Cell Physiology 269, no. 6 (December 1, 1995): C1557—C1564. http://dx.doi.org/10.1152/ajpcell.1995.269.6.c1557.
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Quantitative confocal microscopy methods are used to measure events along the crypt-to-surface axis in living mouse colonic mucosa. Experiments visualize carboxyseminaphthorhodofluor-1 (SNARF-1; a pH-sensitive fluorescent dye) in the extracellular fluid to measure extracellular pH within an intact epithelium. Lucifer yellow (LY; a pH-insensitive dye) is used to control for fidelity of the optical path. Light scatter from colonic tissue caused SNARF-1 or LY fluorescence to decrease 3% per micrometer focal distance into tissue at both 640- and 580-nm emission wavelengths. However, dual emission ratios of LY fluorescence were constant as a function of focal distance into tissue or in the presence of short-chain fatty acids (SCFA). SCFA, known to cause changes in extracellular pH, cause maximal changes in crypt luminal pH at 10 microns from the crypt base. Maximal changes of pH in lamina propria occur higher along the crypt-to-surface axis than maximal changes in luminal pH. Lateral intercellular spaces between colonocytes are a separate microdomain in which pH is constant in absence vs. presence of SCFA and along the base-to-apex axis of colonocytes.
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ICHIKAWA, TOSHIO. "Architecture of Cerebral Neurosecretory Cell Systems in the Silkworm Bombyx Mori." Journal of Experimental Biology 161, no. 1 (November 1, 1991): 217–37. http://dx.doi.org/10.1242/jeb.161.1.217.
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Anatomical and physiological characteristics of putative neurosecretory cells (NSCs) in the medial and lateral areas of the larval brain of Bombyx mori, identifiable by the opalescent appearance of their somata, were examined by means of intracellular recording and staining. Intracellular injection of Lucifer Yellow revealed that the medial cell group consisted of at least six subgroups of cells distinguishable by the geometry of their dendritic branches. Five subgroups of cells project axons to the contralateral corpus allatum (CA) or to the corpus cardiacum (CC). The remaining subgroup sends an axon to the ipsilateral ventral nerve cord. Three subgroups of cells were identified in the lateral group, projecting axons to the ipsilateral CC, to the CA or to the contralateral CA. Large and prolonged action potentials, similar to those recorded in some neurosecretory systems, were recorded from these medial and lateral cells. However, two pairs of medial cells containing paraldehyde-fuchsin-positive (neurosecretory) material and with axons extending to the contralateral nerve cord had action potentials of a short duration, more typical of non-NSCs such as tritocerebral cells innervating the stomodeal dilator muscles via the CC.
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Ewald, D. A., and S. D. Roper. "Intercellular signaling in Necturus taste buds: chemical excitation of receptor cells elicits responses in basal cells." Journal of Neurophysiology 67, no. 5 (May 1, 1992): 1316–24. http://dx.doi.org/10.1152/jn.1992.67.5.1316.
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1. Taste cells in intact taste buds in slices of Necturus lingual epithelium were impaled with microelectrodes for intracellular recording. Two types of cells were investigated: taste receptor cells and basal cells. 2. Impaling cells in the apical end of taste buds resulted in intracellular records from taste receptor cells. Applying short pulses (100- to 200-ms duration) of 140 mM KCl solution to the apical pore elicited receptor potentials in the taste receptor cells. 3. Impaling cells in the base of the taste bud resulted in intracellular records from taste receptor cells and basal cells. KCl applied to the taste pore elicited responses in the basal region that varied greatly in both magnitude and time of onset. The latency of these responses (time of onset compared with the onset of the receptor potential) ranged from 0 to hundreds of milliseconds. 4. Impaled cells were identified by injecting Lucifer yellow after recording KCl responses for 21 cells. KCl responses recorded from identified basal cells all had latencies of greater than 75 ms. KCl responses from identified receptor cells all had latencies of less than 75 ms. 5. One explanation for the long latency of KCl responses recorded in basal cells is that the responses represent postsynaptic potentials. In agreement with this interpretation, long-latency responses, but not short-latency responses, were reversibly reduced by the Ca antagonist Cd (1 mM, 10- to 20-min bath exposure). 6. Long-latency responses also differed from short-latency responses in their voltage dependence. Short-latency responses had the same voltage dependence as apically recorded receptor potentials, increasing with hyperpolarization from resting potential with an extrapolated reversal potential near 0 mV. Long-latency responses were much less dependent on voltage in this range. 7. We measured the spread of exogenously applied KCl with potassium-sensitive electrodes. Long-latency responses were not generated by diffusion of applied KCl to the basal region of the taste bud. A small transient increase in extracellular potassium occurred at the base of the taste bud after chemostimulation at the apical pore. This increase was due to depolarization-evoked release of potassium from taste cells and did not cause the long-latency responses in basal cells. 8. We conclude that short-latency (less than 75 ms) responses recorded from cells situated in the bases of taste buds are electrotonically conducted receptor potentials generated at the apical region. Long-latency (greater than 75 ms) responses are consistent with recording postsynaptic responses in basal cells.
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Teodoro, Sarah de Souza Alves, Maria Lúcia Negreiros-Fransozo, Sabrina Morilhas Simões, Mateus Lopes, and Rogerio Caetano da Costa. "Population ecology of the planktonic shrimp Lucifer faxoni Borradaile, 1915 (Crustacea, Sergestoidea, Luciferidae) of the southeastern coast of Brazil." Brazilian Journal of Oceanography 60, no. 2 (June 2012): 245–53. http://dx.doi.org/10.1590/s1679-87592012000200014.
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Luciferid shrimps have short life spans and a rapid turnover of generations, engage in sequential spawning, and protect their eggs during incubation. This study investigates the ecology of Lucifer faxoni Borradaile, 1915 in the littoral zone, Ubatuba region, São Paulo. Sampling was conducted monthly from July 2005 to December 2006 using a Renfro net trawled over a distance of 50 m for a total sampling effort of 50 m² at each station. Nine stations were sampled, ranging from 1 to 15 m deep. Three stations each were grouped into zones 1, 2 and 3 (Z1, Z2 and Z3). Monthly values of salinity, temperature and rainfall were recorded at each station. The pre-buccal somite length (SL) of each specimen was measured. The results showed that in shallower zones (Z1 and Z2), 6306 individuals were captured, whereas in the deeper zone (Z3), 3808 specimens were captured, but no significant differences in SL was detected between the specimens from Z1 and Z2 and those from Z3 (ANOVA, p=0.25). The abundance of shrimps did not differ significantly between seasons (Tukey's test, p=0.02) except in the spring. The sex ratio differed significantly over the seasons (χ², p<0.05). The results were closely associated with environmental factors with respect to the spatial and seasonal distribution of L. faxoni. Rainfall affected salinity directly, and contributed to the displacement of these shrimps to deeper areas.
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Meulemans, W., and A. De Loof. "Transport of the cationic fluorochrome rhodamine 123 in an insect's Malpighian tubule: indications of a reabsorptive function of the secondary cell type." Journal of Cell Science 101, no. 2 (February 1, 1992): 349–61. http://dx.doi.org/10.1242/jcs.101.2.349.
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The pathway of rhodamine 123 was examined after injection into Sarcophaga flies and after in vitro labeling of the Malpighian tubules. After in vitro labeling the primary cells only retained this potential-sensitive dye for a short period while all secondary cells accumulated the dye from the tubule lumen. In vivo the secondary cells also accumulated rhodamine 123 from the lumen, but the primary cells in the distal parts of all four tubules retained the dye for prolonged periods. This was most pronounced in the distal part of the anterior Malpighian tubules, where rhodamine 123 was eventually precipitated on the luminal concretions. Rhodamine 123 initially accumulated in the secondary cell mitochondria and eventually in intensely fluorescing vesicles, probably lysosomes. No evidence for endocytotic processes from the lumen was found using Lucifer Yellow CH, fluorescent dextrans and fluorescent albumin. Prior incubation with the ionophores valinomycin, nigericin, CCCP (all 1 micrograms/ml), dinitrophenol (1 mM) and NaN3 (10(−2) M) inhibited the selective accumulation of rhodamine 123 to a large extent while monensin (1–5 micrograms/ml) showed little inhibitory effect. Furthermore, only cationic and no anionic or neutral dyes were accumulated by the secondary cells. In the fleshfly Calliphora and the fruitfly Drosophila, the dye rhodamine 123 also selectively accumulated in the secondary cells, as well in vitro as in vivo.
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PRZYSIEZNIAK, JAN, and ANDREW N. SPENCER. "Primary Culture of Identified Neurones from a Cnidarian." Journal of Experimental Biology 142, no. 1 (March 1, 1989): 97–113. http://dx.doi.org/10.1242/jeb.142.1.97.
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Several types of neurones were dissociated from the nerve-rings of the hydrozoan jellyfish Polyorchis penicillatus, using collagenase digestion preceded, and if necessary followed, by removal of external divalent cations. The neurones were cultured for up to 2 weeks in artificial sea water, on a mesogloeal substratum. One subset of large neurones, the swimming motor neurones (SMNs; soma approx. 20×50 μm), exhibited distinct morphological features in vitro, such as large size, wide processes, clear cytoplasm and membranous inclusions around the nucleus. These neurones retained their characteristic action potential shape in culture, with spikes measuring 50±11 mV (N=18) in peak amplitude and 37 ± 11 ms in duration. SMNs could be labelled in vivo with carboxyfluorescein or Lucifer Yellow, subsequently dissociated, and identified in vitro. Two subsets of small neurones were also identifiable. One exhibited electrophysiological similarities with B system neurones, known to be presynaptic to the SMNs in vivo, showing a burstlike pattern of spikes of short duration (5.4 ± 1.4 ms; N=6) and small amplitude (25 ± 7mV). Another subset of small neurones could be labelled with antiserum against the carboxy-terminal peptide moiety, Arg-Phe-amide. Biophysical and neurotransmitter studies at the level of the single identified hydrozoan neurone will be easier in isolated cell culture. This approach will avoid problems encountered in studying the semidissected nerve-ring preparation.
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STEPHENS, PHILIP J. "Electrical and Chemical Synapses Between Giant Interneurones and Giant Flexor Motor Neurones of the Hermit Crab (Pagurus Pollicaris)." Journal of Experimental Biology 123, no. 1 (July 1, 1986): 217–28. http://dx.doi.org/10.1242/jeb.123.1.217.
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1. An examination is made of the characteristics of the synapses between the single pair of giant interneurones (GIs) and the giant flexor motor neurones (GFMNs) in the fused thoracic-abdominal (TA) ganglion of the hermit crab Pagurus pollicaris. 2. There is an electrical synapse between each GI and its ipsilateral GFMN. Evidence for this includes (a) dye (Lucifer Yellow CH) coupling between the two neurones, (b) a short synaptic (0.2 ms) delay between spikes in the two axons, (c) the ability to pass hyperpolarizing current between the two neurones and (d) the sensitivity of the connection to bath applications of N-ethylmaleimide. This synaptic connection is rectifying, since a GFMN spike does not provoke an action potential in the GI. 3. There is a connection between the GI and the contralateral GFMN. Data indicating that this synaptic connection is chemical includes (a) a synaptic delay of between 0.6 and 0.8 ms, (b) transmission i9 easily and irreversibly fatigued, (c) the synapse is insensitive to N-ethylmaleimide and (d) there is no dye coupling between the two neurones. 4. Branches of the GFMN come in close proximity with the GI on both sides of the TA ganglion. However, it is not known whether there is a direct connection or an intervening neurone between the GI and the contralateral GFMN.
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Krupa-Małkiewicz, Marcelina, and Piotr Żurawik. "In vitro rooting of Crocosmia × crocosmiiflora ‘Lucifer’." Folia Horticulturae 29, no. 1 (June 27, 2017): 3–10. http://dx.doi.org/10.1515/fhort-2017-0001.
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Abstract The aim of this study was to determine the effect of various auxins and their concentrations on Crocosmia × crocosmiiflora ‘Lucifer’ rooting in in vitro cultures and in vivo conditions. The initial research materials were shoots obtained from a stabilized in vitro culture. MS media were supplemented with the following auxins: IAA, IBA and NAA at concentrations of 0.5, 1.0, 2.0 and 5.0 mg dm-3. The results of the study showed that hormone applications had a positive effect on the root development of Crocosmia microshoots. 100% rooted shoots were observed in each of the applied combinations of the experiment. The highest number of roots and the longest roots were formed in the presence of IBA at a concentration of 1.0 mg dm-3. IAA, IBA and NAA affected plant height and root morphology of Crocosmia differently. The addition of higher concentrations of auxins to the medium (2.0 and 5.0 mg dm-3) resulted in the formation of short roots. The applied IBA concentrations determined the percentage of the plants that were adapted to in vivo conditions, as well as their vegetative features and the yield of descendant tubers. At the end of the vegetation period, higher IBA concentrations, i.e., 1.0, 2.0 and 5.0 mg dm-3, caused a decrease of up to 50-75% in the obtained plants and also inhibited plant height, as well as the weight and diameter of the tubers formed, compared to the control.
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Lampe, P. D. "Analyzing phorbol ester effects on gap junctional communication: a dramatic inhibition of assembly." Journal of Cell Biology 127, no. 6 (December 15, 1994): 1895–905. http://dx.doi.org/10.1083/jcb.127.6.1895.
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The effect of 12-O-tetradeconylphorbol-13-acetate (TPA) on gap junction assembly between Novikoff hepatoma cells was examined. Cells were dissociated with EDTA to single cells and then reaggregated to form new junctions. When TPA (25 nM) was added to the cells at the onset of the 60-min reaggregation, dye transfer was detected at only 0.6% of the cell-cell interfaces compared to 72% for the untreated control and 74% for 4-alpha TPA, an inactive isomer of TPA. Freeze-fracture electron microscopy of reaggregated control cells showed interfaces containing an average of more than 600 aggregated intramembranous gap junction particles, while TPA-treated cells had no gap junctions. However, Lucifer yellow dye transfer between nondissociated cells via gap junctions was unaffected by 60 min of TPA treatment. Therefore, TPA dramatically inhibited gap junction assembly but did not alter channel gating nor enhance disassembly of preexisting gap junction structures. Short term TPA treatment (< 30 min) increased phosphorylation of the gap junction protein molecular weight of 43,000 (Cx43), but did not change the cellular level of Cx43. Cell surface biotinylation experiments suggested that TPA did not substantially reduce the plasma membrane concentration of Cx43. Therefore, the simple presence of Cx43 in the plasma membrane is not sufficient for gap junction assembly, and protein kinase C probably exerts an effect on assembly of gap junctions at the plasma membrane level.
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Impey, Chris David, Kevin K. Hardegree-Ullman, Anand Patikkal, and Carmen L. Austin. "Teach Astronomy A Comprehensive Online Astronomy Education and Outreach Resource." International Journal for Innovation Education and Research 4, no. 10 (October 31, 2016): 117–27. http://dx.doi.org/10.31686/ijier.vol4.iss10.603.
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A web site called Teach Astronomy (http://www.teachastronomy.com) has been created to serve astronomy instructors and their students, amateur astronomers, and members of the general public interested in astronomy. The content includes astronomy articles from an introductory level textbook and from the online resource Wikipedia, short video clips, astronomical images, podcasts, and recent news stories. This article describes the technology behind the delivery of those learning resources, which is relevant to the capabilities and limitations of the web site. One key innovation is the Wikimap, a Flash-based tool that presents the visual results of a real-time clustering analysis of hundreds or thousands of text items, displaying the item that best matches the search term and most closely related items. The clustering is carried out in a Lucene index, and it can operate on any database containing items of text. The astronomy content is routinely updated, in some cases daily. Due to the prevalence of smartphones, tablets, and other handheld devices, a simplified non-graphical version of the interface was developed using custom style sheets. Teach Astronomy has a large following of students taking introductory astronomy classes and members of the public with a recreational interest in astronomy. In the past year, there have been 250,000 unique visitors. Currently we are developing a new interface that uses HTML5 instead of Flash to display the Wikimap, an app version of the website for use on smartphones and tablets, and tool to support an instructor and learner community.
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Rossi, D. J., S. Alford, E. Mugnaini, and N. T. Slater. "Properties of transmission at a giant glutamatergic synapse in cerebellum: the mossy fiber-unipolar brush cell synapse." Journal of Neurophysiology 74, no. 1 (July 1, 1995): 24–42. http://dx.doi.org/10.1152/jn.1995.74.1.24.
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1. The synaptic activation by mossy fibers (MFs) of unipolar brush cells (UBCs) in the vestibular cerebellum (nodulus and uvula) was examined using patch-clamp recording methods in thin, rat cerebellar slices with Lucifer yellow-filled pipettes for subsequent fluorescence microscopic verification of the cell morphology. 2. UBCs were distinguished from adjacent granule cells in thin cerebellar slices in the uvula and nodulus regions by their larger soma diameters and short dendritic brush, greater whole-cell capacitance, and a prolonged, biphasic excitatory postsynaptic current (EPSC) to stimulation of MFs. 3. Thin-section transmission electron micrographs of the MF-UBC synapse displayed an unusually extensive area of synaptic apposition estimated to measure 12-40 microns2. The majority of UBCs was innervated by a single MF. At high magnification, individual clusters of presynaptic vesicles could be discerned, separated by regions of presynaptic membrane lacking vesicles, but apposed to continuous regions of postsynaptic density. Thus, after release, transmitter diffusion from the synaptic cleft must traverse considerable stretches of postsynaptic membrane before escape into extracellular space. In contrast, MF-granule cell synapses in these cerebellar regions resembled glutamate synapses in other brain regions in that the total synaptic area measured < or = 4 microns2. These synaptic junctions were flanked by short stretches of unspecialized plasma membrane, providing a short (0.5 micron) diffusional path from the site of neurotransmitter release to a branch point of the extracellular space. 4. The MF-evoked EPSC in UBCs was composed of a fast (10-90% rise time: 0.70 ms) and slow (10-90% rise time: 395 ms; 10-90% decay time: 3.1 s) component. The fast component was blocked by the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate (AMPA/KA) antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (10 microM) and displayed linear current-voltage (I-V) relations in the presence or absence of external magnesium. 5. The slow EPSC was also mediated by glutamate receptors, but in most neurons both AMPA/KA and N-methyl-D-aspartate (NMDA) receptors contributed to the slow EPSC, with the contribution of NMDA receptors predominating in the majority of cells. Consequently, although all cells displayed linear I-V relations in Mg(2+)-free saline, cells in which the slow EPSC was predominently mediated by NMDA receptors exhibited voltage-dependent rectification in the presence of external Mg2+ (1 mM). 6. With increasing postnatal age (10-30 d), the contribution made to the slow EPSC by NMDA receptors declined, with a reciprocal increase in the contribution being made by AMPA/KA receptors.(ABSTRACT TRUNCATED AT 400 WORDS)
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Aquino Júnior, Francisco De. "Clodovis Boff e o método da Teologia da Libertação. Uma aproximação crítica." Revista Eclesiástica Brasileira 68, no. 271 (April 5, 2019): 597. http://dx.doi.org/10.29386/reb.v68i271.1428.
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Todo saber que se pretenda crítico está obrigado a dar razão de si mesmo. E disso não está isenta a teologia, pelo menos na medida em que se pretenda crítica. De vez em quando precisa voltar-se sobre si mesma, explicitar seus pressupostos práxico-teóricos, rever o caminho feito, ponderar os resultados, identificar e corrigir possíveis desvios ou deformações, enfim, justificar-se a si mesma para poder seguir adiante com lucidez e vigor. No caso da Teologia da Libertação (TdL), quem mais se confrontou com essa problemática foi Clodovis Boff. O presente artigo retoma, criticamente, sua compreensão e formulação da TdL e de seu método com o intuito de aprofundar, fortalecer e impulsionar um quefazer teológico verdadeiramente libertador.Abstract: Every field of knowledge that claims to be critical must be able to justify itself. Theology is no exception, at least as long as it aspires to be critical. Every now and then it must turn to itself, make its practical-theoretical presuppositions clear, look again at the path already covered, weigh its results, identify and correct possible deviations or distortions, in short, justify itself in order to be able to go forward with clarity of thought and energy. In the case of the Theology of Liberation (TdL), Clodovis Boff was the person who most often had to confront this problem. The present article resumes in a critical manner his understanding and formulation of the TdL and of his own method in the hope of deepening, strengthening and fostering a truly liberating theological work.
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Fukami, Hideyuki, and Robert M. Bradley. "Biophysical and Morphological Properties of Parasympathetic Neurons Controling the Parotid and von Ebner Salivary Glands in Rats." Journal of Neurophysiology 93, no. 2 (February 2005): 678–86. http://dx.doi.org/10.1152/jn.00277.2004.
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The inferior salivatory nucleus (ISN) contains parasympathetic neurons controlling the parotid and von Ebner salivary glands. To characterize the neurophysiological and morphological properties of these neurons, intracellular recordings were made from anatomically identified ISN neurons in rat brain slices. Neurons were also filled with Lucifer yellow and morphometrically analyzed. Based on responses to membrane hyperpolarization followed by depolarization, three types of repetitive discharge patterns were defined for neurons innervating the parotid gland. The regular, repetitive discharge response to membrane depolarization was changed by hyperpolarization resulting either in a delay in the occurrence of the first spike or to an increase in the length of the first interspike interval in the action potential train. Membrane hyperpolarization had little effect on the discharge pattern of some neurons. Similar response discharge patterns were found for neurons innervating the von Ebner salivary gland, which also included a further group of neurons that responded with a short burst of action potentials. Neurons innervating the parotid salivary glands differed morphologically from the von Ebner salivary glands having significantly larger soma and more and longer dendrites than von Ebner gland neurons. In addition, the mean membrane input resistance, time constant, and spike half-width of parotid gland neurons was significantly lower than in von Ebner gland neurons. These differences in intrinsic membrane properties and morphology may relate to the functions of the von Ebner and parotid glands. von Ebner glands are involved in taste stimulus delivery and removal from posterior tongue papillae while the parotid glands contribute saliva to the entire mouth.
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Kim, H. G., and R. F. Miller. "Physiological and morphological correlations of horizontal cells in the mudpuppy retina." Journal of Neurophysiology 67, no. 4 (April 1, 1992): 829–40. http://dx.doi.org/10.1152/jn.1992.67.4.829.
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1. Horizontal cells (HCs) of the mudpuppy (Necturus maculosus) retina were physiologically characterized with the use of intracellular recordings in a superfused, dark-adapted, retina-eyecup preparation. 2. Physiological analysis included an evaluation of rod versus cone input and a determination of the receptive field size with the use of a displaced slit of light. 3. The morphology of HCs was established through intracellular staining with horseradish peroxidase (HRP) and Lucifer yellow mixed in a single electrode. 4. Three types of horizontal cells were identified, each associated with a distinct morphology. Physiological subtypes included luminosity (L) and chromaticity (C) cells. Morphological diversities included single axon-bearing, multiple axon-bearing and, nonaxon-bearing cells. All C-type HCs lacked axons. 5. Approximately 90% of HCs encountered in this study were L-type cells, which received sign-conserving inputs from both rods and cones. These cell types contained one or more long axons that often stretched greater than 500 microns. This group was morphologically diverse, particularly with respect to variations in the number of axons, but we were unable to correlate this diversity with any unique set of physiological properties. 6. Several C-type HCs were identified (n = 8). These cells depolarized to a low-intensity, short-wavelength (SW) stimulus, whereas they hyperpolarized to high-intensity, long-wavelength stimuli. Morphologically, these cells were axonless (n = 4), with relatively small dendritic fields. 7. A third group of HCs were classified as "short wavelength preferring" HCs (n = 7). These cells responded better to a SW stimulus at all intensity levels. They were thus dissimilar to the common L-type HCs, which showed an apparent rod to cone transition as the stimulus intensity increased, suggestive of a shift from rod to cone preference. Morphologically, these cells were axonless (n = 2), but had broader dendritic fields than the C-type HCs. 8. Our observations indicate that the horizontal cell population of the mudpuppy retina is considerably more complex than previously supposed. The existence of both axon-bearing and axonless HCs, which could be correlated with L- and C-type physiology, implies that HCs may support more than one function in outer retina processing.
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Miosge, N., E. Günther, E. Heyder, B. Manshausen, and R. Herken. "Light and electron microscopic localization of the alpha 1-chain and the E1 and E8 domains of laminin-1 in mouse kidney using monoclonal antibodies to establish the orientation of laminin-1 within basement membranes." Journal of Histochemistry & Cytochemistry 43, no. 7 (July 1995): 675–80. http://dx.doi.org/10.1177/43.7.7608521.
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To localize the different domains of the laminin-1 molecule in tissues and gain insight into their in vivo relevance, we raised rat anti-mouse monoclonal antibodies (MAbs) against the entire molecule. Then we tested eight of the 20 clones producing anti-laminin-1 MAbs to specify their reactivity towards the alpha 1-, beta 1-, and gamma 1-chains and the elastase-cleaved fragments of the laminin-1 molecule. We found three MAbs with high titers in ELISA that showed good reactivity in embedded tissue. One of these reacted specifically against the E1 fragment, one against the E8 fragment, and one MAb detected the alpha 1-chain of laminin-1 but not the beta 1- or gamma 1-chain. All three MAbs are useful for light immunohistochemical investigations on cryosections and on paraffin-embedded material, and for ultrastructural localization of laminin-1 in LR Gold-embedded mouse tissue. Antibody staining of the E1 and E8 domains of laminin-1 revealed distinct localization of the molecule in the proximal tubule basement membranes of mouse kidney. The short arms (E1) of the laminin-1 molecule are predominantly located in the lamina lucida and the long arms (E8) are oriented towards the lamina fibroreticularis. Therefore, both MAbs are useful for studies of the orientation of the laminin-1 molecule in basement membranes. The distal tubule basement membranes did not show any distinct pattern of laminin-1 distribution. In general, the distal tubules showed the strongest reactions over the entire width of the basement membrane for all three MAbs. In contrast, the proximal tubule basement membranes showed somewhat weaker reactivity but a distinct pattern of laminin-1 distribution, with the E1 fragments oriented towards the adjacent epithelial cell surface.