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Academic literature on the topic 'Luteus'

Author: Grafiati

Published: 5 June 2025

Last updated: 24 June 2025

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Journal articles on the topic "Luteus"

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García-Rodríguez, José-Leonardo, Jesús Pérez-Moreno, Darcy Ríos-Leal, et al. "In vitro GROWTH OF ECTOMYCORRHIZAL FUNGI ASSOCIATED WITH Pinus radiata PLANTATIONS IN CHILE." Revista Fitotecnia Mexicana 40, no. 4 (2017): 415–23. http://dx.doi.org/10.35196/rfm.2017.4.415-423.

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A comparative study of in vitro growth of three species of ectomycorrhizal fungi (ECMF) (Rhizopogon luteolus, Suillus granulatus and Suillus luteus) was performed. Fungal material was collected in adult Pinus radiata plantations. Isolation and purification of the strains were performed on potato-dextrose-agar medium and the evaluation of the radial growth rate and the increase in mycelial biomass, under different culture conditions, was performed on the Modified Melin Norkrans growth medium. The effects of temperature (24, 28 and 32 ºC) and pH (4.8, 5.3, 5.8, 6.3 and 6.8) of the growth medium were tested for the three fungal species in two independent assays. The results indicate that the temperature had a significant effect on the radial growth rate (RG) and mycelial biomass increase (MB) in all of the evaluated fungal species. The highest RGR and MBI were recorded in R. luteolus, and the lowest values for these variables were registered in S. luteus. Rhizopogon luteolus had the highest sensitivity to pH changes. Meanwhile, there was no pattern in S. granulatus and S. luteus growth response under different pH conditions. When cultivated in vitro, the three studied species of ECMF presented adaptation, exponential, declining and stationary growth phases. The in vitro growth responses recorded in the present study showed the great potential of R. luteolus to be used in future programs using mycorrhizal inoculation in the production of Pinus radiata trees in nurseries in Chile.

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An, Qinghui, Jianghua Zheng, Jingyun Guan, et al. "Predicting the Effects of Future Climate Change on the Potential Distribution of Eolagurus luteus in Xinjiang." Sustainability 15, no. 10 (2023): 7916. http://dx.doi.org/10.3390/su15107916.

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Eolagurus luteus (yellow steppe lemming Eolagurus luteus Eversmann, 1840) is a keystone species in the desert steppe of northern Xinjiang, one of the regions most affected by global climate change. Their behavior of eating grassland vegetation and digging holes has resulted in the reduction of grassland vegetation and soil erosion in northern Xinjiang, which has seriously affected the ecological balance of the grassland in northern Xinjiang, and pathogens carried by E luteus pose a great threat to human health. Climate change exacerbates the uncertainty of the outbreak of E luteus. Predicting the suitable habitat area of this species under climate change scenarios will help farmers and herders deal with the potential threat of an E luteus outbreak. In this study, 117 actual occurrence points of E luteus were used, and 24 climate models, 6 soil factors and 3 topographic factors from the Coupled Model Intercomparison Project (CMIP6) were taken into account. Combining the MaxEnt model and the overlay analysis function of ArcGIS software, the potential geographic distribution of E luteus in 2030 and 2050 for the green development path (SSP126), the intermediate development path (SSP245), the regional competition path (SSP370), and the high development path (SSP585) was predicted. The change trend of the suitable area and distribution pattern of E luteus in Xinjiang under future climate conditions was analyzed, and the main environmental factors affecting the distribution of E luteus are discussed. The results show that the average area under curve (AUC) and true skill statistics (TSS) of the MaxEnt model are 0.993 and 0.8816, respectively, indicating that the model has a good prediction effect. The analysis of environmental factors showed that the main environmental factors affecting the potential geographical distribution of E luteus are average annual temperature, isotherm, average temperature in the wettest quarter, average temperature in the driest quarter, and precipitation variation coefficient. With the increase of radiation intensity and time, the suitable areas of E luteus will continue to decrease. Especially in the 2050s under the SSP585 scenario, the middle and high suitable areas will decrease by 2.58 × 104 km2 and 1.52 × 104 km2, respectively. Although the potential habitat area of E luteus is shrinking, the future threat of E luteus to grassland ecological security and human health should not be underestimated due to ecological adaptation of the community and the frequent occurrence of extreme weather. Therefore, studying changes in the potential geographic distribution of E luteus under climate change scenarios and developing appropriate monitoring programs are of great importance for grassland ecological security and human health. This study fills in the gaps in the study of the potential geographical distribution of E luteus and provides methodological and literature support for the study of the potential geographical distribution of other rodents.

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Dixon, Robert, and Paul Johnson. "Synthesis of Ectomycorrhizae on Northern Red Oak Seedlings in a Michigan Nursery." Arboriculture & Urban Forestry 18, no. 5 (1992): 266–72. http://dx.doi.org/10.48044/jauf.1992.052.

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Vegetative inoculum of the ectomycorrhizal fungus Suillus luteus was thoroughly mixed into fumigated nursery soil, and northern red oak seedlings of four families were evaluated one and two years after sowing for ectomycorrhizal development, growth, and nutrition. At the end of year 1, treated seedlings were successfully inoculated with S. luteus, but the percentage varied significantly with family. Suillus luteus persisted on lateral roots two years following sowing. Two of four seedling families inoculated with S. luteus were significantly larger in size than control plants. These results suggest that the fungal symbiont S. luteus can be successfully introduced into nurseries and that early ectomycorrhizal development improves the growth of northern red oak seedlings.

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Achmad, Ayu Ashari, M. Saifur Rohman, and Irfan D. Prijambada. "Biochemical Properties of Crude Extracellular Proteases from Chromohalobacter salexigens BKL5 and Micrococcus luteus 11A." Indonesian Journal of Biotechnology 21, no. 1 (2017): 56. http://dx.doi.org/10.22146/ijbiotech.26705.

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In this work, we have reported an enzymatic activity and biochemical properties of extracellular proteases from Chromohalobacter salexigens BKL5 and Micrococcus luteus 11A. C. salexigens BKL5 and M. luteus 11A were previously isolated from Bledug Kuwu mud volcano and dietary industry wastewater treatment, respectively. Both bacterial strains were able to produce extracellular proteases, when grown on minimal agar medium supplemented with 1% of skim milk. Proteolytic indexes of C. salexigens BKL5 and M. luteus 11A were 2.5±0.14 and 2.9±0.42, respectively. Both extracellular proteases exhibited optimum enzymatic activity at pH 7, with specific activity of C. salexigens BKL5 was 13.3% higher than that of M. luteus 11A. Optimum temperature for enzymatic activity of both proteases was 45°C. Metal cofactor preferences assay showed that extracellular protease from C. salexigens BKL5 preferred Zn2+, meanwhile extracellular protease from M. luteus 11A mainly preferred Ca2+ ion. Metal cofactor preferences assay also suggested that crude extracellular protease from C. salexigens BKL5 was categorized as metalloprotease, meanwhile crude extracellular protease of M. luteus 11A was common neutral protease. The enzymatic stability assay against various salt concentrations showed that crude extracellular protease from C. salexigens BKL5 was more stable than that of M. luteus 11A.

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Jain, Rohit, Abigail L. Voss, John R. Tagg, and John D. F. Hale. "Evaluation of the Preliminary Safety, Tolerability and Colonisation Efficacy of Topical Probiotic Formulations Containing Micrococcus luteus Q24 in Healthy Human Adults." Cosmetics 9, no. 6 (2022): 121. http://dx.doi.org/10.3390/cosmetics9060121.

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Probiotics developed for topical applications in humans have the potential to beneficially modulate microbial imbalances on the skin surface and thereby improve skin health. This study was conducted to determine whether topical formulations containing the human skin commensal Micrococcus luteus strain Q24 (BLIS Q24) are safe, tolerable and efficacious when used by healthy human subjects. M. luteus Q24 was assessed in vitro for haemolytic activity and its antibiotic susceptibility profile. Formulations of strain Q24 were evaluated for the preliminary safety and tolerability in healthy human participants. Forty-seven adults were randomly assigned to four single-site, single-blind randomised placebo or baseline controlled or active-controlled trials. Skin swab samples were collected for differential viable counts to monitor levels of probiotic colonisation. M. luteus Q24 was found to be non-haemolytic and susceptible to commonly used antibiotics. The M. luteus Q24 formulations were safe and tolerable and >90% of the participants reported improvements from baseline in the appearance (e.g., radiance and hydration) of their treated skin. Additionally, participants observed a reduction in pore size, skin clarity and enhanced skin softness. No adverse effects were reported. A dose-related significant increase was observed in the levels of M. luteus Q24 isolated from skin swabs of the probiotic-treated subjects. Placebo-controlled trials in human subjects involving the topical application of different doses of M. luteus Q24 formulations were supportive of the safety, tolerability and efficacy of probiotic M. luteus Q24. Self-reported skin health assessments by the subjects indicated that M. luteus Q24 has good potential as a probiotic for improving skin health quality.

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Papazov, Penko, and Panteley Denev. "DETERMINATION PHOSPHOLIPIDS OF WILD EDIBLE MUSHROOMS (SUILLUS LUTEUS)." Applied Researches in Technics, Technologies and Education 6, no. 4 (2018): 311–20. http://dx.doi.org/10.15547/artte.2018.04.005.

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Mushroom samples were collected in 2017 and 2018 from the Batak mountain by the authors themselves. The aim of this study was to determine phospholipids composition of species wild edible mushrooms (Suillus luteus) grown in the Batak mountain, Bulgaria. Phosphatidic acids were the main components in lipids from Suillus luteus (34.2 %). The content of the other phospholipids in the oils from Suillus luteus was from 8.4 to 19.6%. Phosphatidylcholine was identified in Suillus luteus (19.6 %). Based on the results, can be concluded that the examined species of mushrooms is a potential source of phospholipids and can be used as a functional food or as food additives in many products.

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Chemerovska, I. O. "Research on the microflora of cattle and deer during the development of wounds, abscesses and endometritis." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 27, no. 117 (2025): 158–65. https://doi.org/10.32718/nvlvet11722.

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To date, pathogenic and opportunistic microorganisms are common. From the biological material collected from the site of abscess development, the predominant microflora were in cattle: Micrococcus luteus, Esherichia coli, Enterococcus faecalis, Staph. epidermidis, Bac. subtilis, Staph. intermedius, Staph. aureus, Streptococcus spp, Staph. haemolyticus, Staph. eguorum, Staph. cohnii, Staph. felis, Corinebacterium xerosis, Kocuria rhizophia, Enterobacter spp: Micrococcus luteus, Staph. epidermidis, Bac. megaterium, Staphylococcus spp. Streptococcus spp. Pseudomonas aeruginosa, Enterococcus faecalis, Esherichia coli. From the biological material collected from the site of wound development, the predominant microflora were in cattle: Bac. megaterium, Acinetobacter spp. 17.65 %, Enterococcus faecalis, Pseudomonas spp: Staph. epidermidis, Staphylococcus spp., Micrococcus luteus, Streptococcus spp., Staph. aureus, Bac. subtilis, Proteus spp., Enterococcus faecalis, Esherichia coli, Pseudomonas aeruginosa, Staph. simulans, Staph. intermedius, Staph. sciuri, Acinetobacter spp., Pseudomonas spp. The predominant microflora of the biological material collected during the development of endometritis were in cattle: Micrococcus luteus, Enterococcus faecalis, Staph. aureus, Staph. chromogenes, Pseudomonas aeruginosa, Staph. haemolyticus, Staph. gallinarium, Staph. simulans, Staph. eguorum, Streptococcus spp. From the results obtained, it can be concluded that in cattle we most often identified Micrococcus luteus – 15.39 % and Enterococcus faecalis – 13.46 % in the study of endometritis, Micrococcus luteus – 23.53 %, Staphylococcus spp. – 23.53 %, but for the study of abscesses of Micrococcus luteus – 20 %, Escherichia coli – 13.33 % of deer for the study of wounds of Escherichia coli – 21.05 %, Staph. aureus – 15.79 % of wounds, and abscesses of Staph. aureus – 31.81 %, Escherichia coli – 27.27 %.

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Pederson, Addie, R. Boone Coleman, and Mary Irving. "An unknown source of Micrococcus luteus bacteremia." Southwest Respiratory and Critical Care Chronicles 11, no. 48 (2023): 43–45. http://dx.doi.org/10.12746/swrccc.v11i48.1187.

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Micrococcus luteus is a common organism in the human skin flora. It is an uncommon cause of pathogenic bloodstream infections, which occur mostly in immunocompromised patients. We report the clinical course of an 82-year-old man who underwent complex management of multiple diverticular abscesses and had three serial positive blood cultures for M. luteus. Despite treatment with vancomycin, the patient ultimately died due to septicemia secondary to mixed gastrointestinal flora and M. luteus. A source this infection was never definitely identified.

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Usó, Jordi, María Gil, Bárbara Gomila, and Mª Dolores Tirado. "Endocarditis por Micrococcus luteus." Enfermedades Infecciosas y Microbiología Clínica 21, no. 2 (2003): 117. http://dx.doi.org/10.1157/13042871.

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Usó, Jordi, María Gil, Bárbara Gomilab, and M. Dolores Tiradob. "Endocarditis por Micrococcus luteus." Enfermedades Infecciosas y Microbiología Clínica 21, no. 2 (2003): 117. http://dx.doi.org/10.1016/s0213-005x(03)72895-x.

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Dissertations / Theses on the topic "Luteus"

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Lauer, Iris. "Untersuchung der Strukturdynamik arbeitender F1-ATPase und ATP-Synthase aus Micrococcus luteus in Einzelschuss-Experimenten mit Synchrotronstrahlung." [S.l. : s.n.], 2001. http://ArchiMeD.uni-mainz.de/pub/2001/0127/diss.pdf.

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OLIVEIRA, Louise Melo de Souza. "Atividades biológicas de metabólitos de cactaceae e Micrococcus luteus." Universidade Federal de Pernambuco, 2017. https://repositorio.ufpe.br/handle/123456789/26927.

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Submitted by Pedro Barros (pedro.silvabarros@ufpe.br) on 2018-09-13T21:32:55Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Louise Melo de Souza Oliveira.pdf: 1478143 bytes, checksum: 5477c219843e225280059e0100acaa49 (MD5)<br>Rejected by Alice Araujo (alice.caraujo@ufpe.br), reason: Por favor, atualizar com novo arquivo que está no banco. Obrigada! on 2018-09-25T22:03:27Z (GMT)<br>Submitted by Pedro Barros (pedro.silvabarros@ufpe.br) on 2018-09-25T22:15:31Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Louise Melo de Souza Oliveira.pdf: 1697579 bytes, checksum: 37970b02f8a78c64db77c1f4f7dded6f (MD5)<br>Approved for entry into archive by Alice Araujo (alice.caraujo@ufpe.br) on 2018-09-25T22:18:05Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Louise Melo de Souza Oliveira.pdf: 1697579 bytes, checksum: 37970b02f8a78c64db77c1f4f7dded6f (MD5)<br>Made available in DSpace on 2018-09-25T22:18:05Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) TESE Louise Melo de Souza Oliveira.pdf: 1697579 bytes, checksum: 37970b02f8a78c64db77c1f4f7dded6f (MD5) Previous issue date: 2017-05-29<br>FACEPE<br>Caatinga é um bioma com características distinta, encontrada apenas no território brasileiro, onde são encontradas muitas espécies endêmicas. A busca por novas moléculas biotivas é crescente, e os produtos naturais desempenham um papel significativo na procura por novos agentes terapêuticos. As Cactaceas que sobrevivem no ambiente da caatinga possuem adaptações anatômicas, fisiológicas e consequentemente modificações no metabolismo secundário. Micrococcus luteus é uma actinobactéria produtora do carotenoide amarelo, sarcinaxantina, esses pigmentos têm importantes funções biológicas, principalmente antioxidante. Neste contexto esse trabalho teve o objetivo de realizar uma revisão bibliográfica da família Cactaceae, analisar a atividade antimicrobiana e antioxidante de Cereus jamacaru, e identificar o pigmento carotenoíde de M. Luteus. Realizou-se um levantamento das espécies das cactaceas encontradas na região de Caatinga do Brasil, através de uma análise fitoquimica e farmacológica, evidenciando as principais atividades biológicas já descritas para essa família. Apesar do número reduzido de trabalhos observou-se que este grupo possui atividade antimicrobiana, antiviral, antiparasitária e antioxidante. Cereus jamacaru (mandacaru) é uma espécie pouco estudada do ponto de vista químico e farmacológico. Neste trabalho foi encontrado, em extrato metanólico, a presença de flavonoídes, fenilpropanoídes, monoterpenos, triterpeno e esteroides, possuindo atividade bactericida contra: Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, Streptococcus mitis, Escherichia coli e Staphylococcus aureus. Apresentou também atividade antioxidante pelo método DPPH (2,2- difenil-1-picril-hidrazila) e ABTS(2,2-azino-bis-(3-etil-benzotiazolina-6-ácido sulfônico). Duas cepas de M. luteus foram isolados do solo e identificadas por análises bioquímicas e moleculares. O pigmento foi caracterizado através de Cromatografia Líquida de Alta Eficiência (HPLC) e identificado como sarcinaxantina. Verificou-se também que este carotenoide possui atividade antioxidante, antimicrobiana e fator de proteção solar.<br>Caatinga is a biome with distinct characteristics, found only in Brazilian territory, and many endemic species are found. The search for new bioactive molecules is increasing, and natural products play a significant role in the search for new therapeutic agents. Cactaceae that survive in the caatinga environment have anatomical, physiological and consequent modifications in secondary metabolism. Micrococcus luteus is an actinobacterium producing the yellow carotenoid, sarcinaxanthin, these pigments have important biological functions, mainly antioxidant. In this context, the objective of this work was to carry out a bibliographic review of the Cactaceae family, to analyze the antimicrobial and antioxidant activity of Cereus jamacaru, and to identify the carotenid pigment of M. luteus. A survey of the species of cactaceae found in the Caatinga region of Brazil was carried out through a phytochemical and pharmacological analysis, evidencing the main biological activities already described for this family. Despite the small number of studies, it was observed that this group has antimicrobial, antiviral, antiparasitic and antioxidant activity. Cereus jamacaru (mandacaru) is a species little studied from the chemical and pharmacological point of view. In this work, the presence of flavonoids, phenylpropanoids, monoterpenes, triterpenes and steroids was found in methanolic extract, having bactericidal activity against: Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, Streptococcus mitis, Escherichia coli and Staphylococcus aureus. It also showed antioxidant activity by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) and ABTS (2,2-azino-bis- (3-ethyl-benzothiazoline-6-sulfonic acid) method). Two strains of M. luteus were isolated from the soil and identified by biochemical and molecular analyzes.The pigment was characterized by High Performance Liquid Chromatography (HPLC) and identified as sarcinaxanthin. This carotenoid was also found to have antioxidant, antimicrobial and sun protection factor.

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Grechana, O. V., O. O. Saliy, A. G. Serbin, and L. A. Fukleva. "The pharmacognosy investigation of associated coumarins Lupinus luteus L." Thesis, Technical University in Zvolen, 2019. https://er.knutd.edu.ua/handle/123456789/15141.

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The genus Lupinus (Lupinus L.) is attributed to the legumes family (Fabaceae L.). About 1000 herbaceous, semi-bush and shrub, annual, winter and perennial lupines are described. In this paper we focuses on the issue of pharmacognostical study of plants is widely used in animal breeding, is the active substance in the manufacture of dietary supplements - the yellow lupine (Fabaceae). Harvested grass were analyzed after acid hydrolysis. Gas-liquid chromatography was first performed with mass spectrometric detection of hydrolysed raw material of Lupinus luteus L., where 56 compounds were found, of which 35 components were identified. After hydrolysis, the raw material of L. luteus L. contained a number of biologically active substances – fatty acids, alcohols, ketones etc. Two components from the class of true coumarins were identified: coumarin (16.4 mg%) and 6–methylcoumarin (2.7 mg%).

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Florez, Roncancio Victor Julio. "Estudo do controle fotoperiodico e hormonal da floração em Solidago X luteus." [s.n.], 1997. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315342.

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Orientador: Maria de Fatima Domingos Aleixo Pereira<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-07-22T08:12:42Z (GMT). No. of bitstreams: 1 FlorezRoncancio_VictorJulio_D.pdf: 5014406 bytes, checksum: 8225825b1d38abb07d08b9566454e2b4 (MD5) Previous issue date: 1997<br>Resumo: O efeito do fotoperíodo sobre o desenvolvimento vegetativo e a indução floral em Aster ericoides cv. Montecasino, Solidago chilensis M. e Solidago x luteus foi estudado em experimentos preliminares. Verificou-se que, de forma geral, os DL (20h) promoveram a indução floral e o aumento no número de ramificações laterais e de folhas, ao passo que, sob DC (8h) , as plantas de S. chilensis permaneceram em roseta, as de S. x luteus tiveram indução e antese floral, enquanto que em A. ericoides havia plantas em roseta e plantas induzidas. Em experimento sazonal com s. x luteus em diferentes fotoperíodos observou-se interação fotoperíodo-temperatura, pois, em fotoperíodos curtos (8h a 14h) e em NI prevaleceu o efeito da temperatura, permanecendo as plantas em roseta em "temperaturas baixas" (outono-inverno) e havendo indução floral em "temperaturas altas" (verão-outono), porém, com altura de caule inferior a 0,45m; ao passo que, em fotoperíodos longos(16h, 18h, 20h e LC), prevaleceu o efeito de indução floral do fotoperíodo, havendo alongamento caulinar próximo a O,90m mesmo em "temperaturas baixas". O tratamento de vernalização das estacas manteve as plantas em roseta em DC e retardou a indução floral nas plantas sob DL. Quando há "bolting" no verão-outono, nas plantas mantidas em fotoperíodos de 8h e NI, há um acúmulo de massa seca na raiz, semelhante ao da parte aérea. Em fotoperíodos, onde foi observada uma menor intensidade resposta indutiva de floração (8h, 10h, 12h, 14h e NI) e "temperatura baixa", ocorreu direcionamento preferencial de assimilados para a raiz. Nas melhores condições indutoras de floração (16h, 18h e 2Oh), que independem da temperatura, verificou-se direcionamento de fotoassimilados para a parte aérea. Como a indução floral ocorreu sob todos os fotoperíodos estudados, verificando-se diferenças quantitativas entre os diferentes fotoperíodos, o híbrido em estudo ficou caracterizado como uma PDL quantitativa, com fotoperíodo mais favorável para indução floral em torno de 16h; contudo, a antese floral foi antecipada em fotoperíodos curtos (8h, 10h e 12h) , refletindo também uma resposta quanti tativa no desenvolvimento floral. Não foi detectada atividade giberelínica em nenhum dos tratamento estudados. Foi constatada maior concentração de AIA em folhas e botões florais de plantas em DL em comparação aos DC, o que poderia estar relacionado à maior velocidade de antese floral em plantas sob DC. As maiores concentrações de ABA, em relação ao início dostratamentos fotoperiódicos ocorreram em folhas e botões florais de plantas em DC; assim, no balanço AIA:ABA, estes estariam em concentrações opostas, principalmente no botão floral em DC, onde se observou a maior quantidade de ABA e a ausência de AIA. A atividade citocinínica, detectada nos extratos provenientes de folhas de plantas sob DC e de botões florais de plantas no início dos tratamentos, sob DL e DC, associado ao fato de o tratamento com KI, em botões florais de plantas em DL, ter acelerado a antese floral, foram as evidências de que citocininas endógenas estariam envolvidas no processo; porém, entre as quatro citocininas dosadas (Z, ZR, iP e iPA) somente o iP e iPA foram detectados. Uma diminuição significativa de iP em folhas de plantas sob DC pode estar sendo estimulada por uma taxa de transporte mais ativa na forma de iPA para o botão floral, o que, por sua vez, estimularia a maior velocidade de antese floral observada sob DC<br>Abstract: The effect of photoperiod on vegetative development and floral induction in Aster ericoides cv. Montecasino, Solidago chilensis M. and Solidago x luteus was studied in the preliminary experiments. Floral induction was generally promoted, and the number of lateral branches and leaves increased under long days (20h). The S. chilensis plants remained rosettes, while in S. x luteus floral induction occurred under short days (8h). However, A. ericoides showed both responses, rosettes and floral induction. In seasonal experiments with different lengths of photoperiods the habit of S. x luteus changed as a result of interaction photoperiod-temperature. In plants under short photoperiods (8h to 14h) and night break the temperature effect prevailed: the plants remained rosettes in the autumn-winter period, and the bolting was promoted by the summer-autumn temperatures with stem elongation not superior to O.45m. In the longer photoperiods (16h, 18h, 20h and continuous light) the photoperiodic effect prevailed, promoting floral induction with stem elongation near to O.90m even in the autumn-winter temperatures. The vernalization of cuttings kept the plants as rosettes under 8h, and delayed the floral induction in plants under 18h. In plants in which bolting was promoted in summer-autumn temperatures, under photoperiods of 8h and night break, root and shoot partitioning of dry matter occurred. Autumn-winter season and photoperiods with less intensive induction response (8h, 10h, 12h, 14h and night break) resulted in accumulation of dry matter in the roots, while in both seasons, autumn-winter and summer-autumn, shoot diversion of assimilates was found under longer photoperiods (16h, 18h and 20h). As floral induction occurred under all photoperiods, but with a more rapid and intense response being recorded under photoperiods next to 16h, this hybrid was characterized as a quantitative long-day plant, although the rate of flower bud development to anthesis was more rapid in the shorter photoperiods (8h, 10h, 12h). In any treatment, the activity of gibberellin-like substances was not detected. In long-day (18h) plants, it was observed a higher concentration of AIA in leaves and floral buds than in short-day (8h) plants, suggesting that it may be related to a more rapid anthesis in plants kept in short days. In extracts from leaves and flower buds in short-day plants the higher levels of ABA were found in relation to the beginning of the photoperiodic treatments. Therefore, it seems to exist an indirect relation between the levels of AIA and ABA mainly in the flower buds under 8h, in which the higher amount of ABA is coincident to disappearance of AIA. Substances with cytokinin activity were detected in leaves of plants kept in short days, and in flower buds of plants at the beginning of the photoperiodic treatments, and plants kept in long days and in short days, along with the fact that the kinetin treatment accelerated the development of anthesis of flower buds of plants under long days, point to a possible role of the endogenous cytokinins in the process. The levels of zeatin-type (Z, ZR) and isopentenyladenosine-type (iP, iPA) cytokinins were examined but only iP and iPA were detected. The decrease in the iP content of plants kept in short days could be the consequence of a more active transport rate of iPA, speeding up the flower bud anthesis in the plants under short days<br>Doutorado<br>Doutor em Ciências Biológicas

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Ruiz, Posadas Lucero del Mar. "Rhizospheric calcium and stomatal behaviour in Lupinus luteus L. and Commelina communis L." Thesis, Lancaster University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296972.

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PIERRE-HEBERT, JOSIANE. "Etapes initiales de la reparation de l'adn modifie par alkylation chez micrococcus luteus." Paris 6, 1986. http://www.theses.fr/1986PA066652.

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Borowska, Magdalena. "Wpływ wybranych regulatorów wzrostu i Ekolistu na plonowanie łubinu żółtego (Lupinus luteus L.)." Rozprawa doktorska, [Nakł.aut.], 2001. http://dlibra.utp.edu.pl/Content/341.

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Badania własne miały określić reakcję łubinu żółtego na regulatory wzrostu oraz mikro - i makroskładniki zastosowane w formie oprysku na początku kwitnienia w celu ograniczenia zjawiska opadania organów generatywnych, a tym samym zwiększenia produkcyjności roślin, wartości pokarmowej nasion, ich żywotności i wigoru. Przedmiotem badań była odmiana łubinu żółtego "Teo"

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Spit, Anthony. "The primary structures and the evolutionary consequences of the olisthodiscus luteus histone proteins." Doctoral thesis, University of Cape Town, 1993. http://hdl.handle.net/11427/21498.

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Bibliography: pages 187-194.<br>During the course of this study, the histones of the algae Olisthodiscus luteus were isolated, purified and fractionated. Identification of the histones was achieved by partial primary structure analysis. The histones Hl, H2A, H2B, H3 and H4 were found to be present in the O. luteus nucleus. The complete structure of H2A and H4 was determined. There is no evidence of the existence of the unique histone HO1 (Rizzo et al., 1985). Construction of phylogenetic trees suggests that the alga Olisthodiscus luteus diverged from the animal line. By sequence comparison, the most closely related histone sequence to the algae was found to be that of the echinodermata. An endosymbiotic event between an echinodermata ancestor and a primitive unicellular alga is hypothesised in an attempt to explain the smilarity between the histones.

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Whitcombe, David Mark. "Molecular cloning and analysis of a β-1,3-glucanase from Arthrobacter luteus (Oerskovia xanthineolytica)". Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/35179.

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Species of Arthrobacter luteus, also known as Oerskovia xanthineolytica, can utilise yeast cells as a growth substrate. This unusual ability is due to the secretion of a battery of hydrolytic enzymes which degrade the yeast cell wall and thus lyse the cells. Although many hydrolytic enzymes are important in the degradation of the yeast cell wall, the key activities are endo beta--l,3-glucanases. In order to characterise components of the yeast lytic system and the genetic organisation of this little-understood organism, a molecular cloning approach was adopted. Large clones expressing beta-1,3-glucanase were isolated from a library of A. luteus DNA constructed in the positive selection vector pKGW. By a combination of subcloning, restriction mapping and Southern analysis, it was determined that the clones contained virtually the same inserts. Additional subcloning, transposon mutagenesis, deletion mapping and nucleotide sequencing were used to identify at least one glucanase gene. The predicted protein product had a molecular weight of about 46 kD. When the gene was expressed in a number of in vivo and vitro systems including E. coli minicells and a Streptomyces coupled transcription/translation system, the protein observed had a similar molecular weight. Furthermore, when the protein was produced in E. coli and run on activity stained gels, the beta-glucanase activity co-migrated with the major glucanase of A. luteus. In addition the E. coli-produced glucanase had the ability to cause limited lysis of yeast.

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Prusiński, Janusz. "Biologiczne i agrotechniczne uwarunkowania rozwoju i plonowania zróżnicowanych genotypów łubinu żółtego (Lupinus luteus L.)." Rozprawa habilitacyjna, Wydaw. Uczelniane Akademii Techniczno-Rolniczej, 1995. http://dlibra.utp.edu.pl/Content/486.

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Books on the topic "Luteus"

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Tomaszewska, Barbara. Rola jabłczanu w metaboliźmie łubinu. Uniwersytet im. Adama Mickiewicza w Poznaniu, 1990.

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Regensburger, Angela. Konstruktion spezifischer Hybridisierungssonden und Sequenzierung eines 23S rRNS-Gens [S-rRNS-Gens] von Micrococcus luteus. [s.n.], 1987.

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Kurz, Christine. Einfluss von Saatzeit, Bestandesdichte und Wasserangebot auf Kornertrag und Ertradsstruktur von Lupinen (L. luteus, L. angustifolius, L. albus, and L. mutabilis). [s.n.], 1988.

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Lutes, Jim. Jim Lutes. Edited by Reimer Karen and University of Chicago. Renaissance Society. The Renaissance Society at the University of Chicago, 2009.

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Lutens, Serge. L'esprit Serge Lutens. Editions Assouline, 1992.

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ill, Cieslawski Steve, ed. The lute's tune. Doubleday, 1992.

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Miegge, Giovanni. Lutero. 4th ed. Editrice Claudiana, 2003.

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Lisman, A. J. A. M., Munnig Schmidt E, Wyck, H. W. M. van der., Stichting "Nederlandse Buitenplaatsen en Historische Landschappen"., and Oudheidkundig Genootschap Niftarlake, eds. Gezigten aan De Rivier de Vecht: Petrus Josephus Lutgens/Lutgers, 24 augustus 1808-19 april 1874. De Stichting Nederlandse Buitenplaatsen en Historische Landschappen, 2001.

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Morel, Nicolas. Lutteurs: Roman. Seuil, 1988.

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Ar-Rehman, Shahid. Sharif Lutere. haider Pub., 2000.

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Book chapters on the topic "Luteus"

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Bährle-Rapp, Marina. "Lupinus Luteus Extract." In Springer Lexikon Kosmetik und Körperpflege. Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_6149.

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Azimova, Shakhnoza S., and Anna I. Glushenkova. "Lupinus luteus L." In Lipids, Lipophilic Components and Essential Oils from Plant Sources. Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_1912.

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Sousa, I. M. N., M. L. Beirão da Costa, S. E. Hill, J. R. Mitchell, and S. E. Harding. "Functional Properties of Lupinus luteus Proteins." In Developments in Food Engineering. Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2674-2_61.

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Iqbal, Muhammad Munir, William Erskine, Jens D. Berger, Joshua A. Udall, and Matthew N. Nelson. "Genomics of Yellow Lupin (Lupinus luteus L.)." In Compendium of Plant Genomes. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-21270-4_11.

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Legocki, A. B., J. Biesiadka, W. Golinowski, et al. "Molecular Genetics of a Model Plant: Lupinus luteus." In Biological Nitrogen Fixation for the 21st Century. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_174.

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Jorjadze, Angelina, Inga Kupradze, Ketevan Batsatsashvili, and Rainer W. Bussmann. "Suillus granulatus (L.) Roussel Suillus luteus (L.) Roussel Suillaceae." In Ethnobotany of Mountain Regions. Springer Nature Switzerland, 2024. https://doi.org/10.1007/978-3-319-50009-6_508-1.

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Seliga, Henryka. "The role of copper in nitrogen fixation in Lupinus luteus L." In Plant Nutrition — from Genetic Engineering to Field Practice. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1880-4_96.

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Ouasti, Imane, Noureddine Chaachouay, Rainer W. Bussmann, and Mostafa Elachouri. "Lupinus albus subsp. albus L.Lupinus angustifolius L.Lupinus digitatus Forssk.Lupinus luteus L.Lupinus pilosus L.Fabaceae." In Ethnobotany of Mountain Regions. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-13933-8_245-1.

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Brennan, Ross F., and Mike D. A. Bolland. "Lupinus luteus cv. Wodjil takes up more phosphorus and cadmium than Lupinus angustifolius cv. Kalya." In Structure and Functioning of Cluster Roots and Plant Responses to Phosphate Deficiency. Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-010-0243-1_14.

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Berdalet, E., M. Latasa, and M. Estrada. "Variations in biochemical parameters of Heterocapsa sp. and Olisthodiscus luteus grown in 12:12 light:dark cycles." In The Daily Growth Cycle of Phytoplankton. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2805-6_12.

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Conference papers on the topic "Luteus"

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Ptashnik, O. P. "Results of the introduction of Lupinus varieties and samples in the Steppe Crimea." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-75.

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Within a framework of Lupinus varieties and samples assessment, we have found that the growing season of Lupinus albus L. was 93, Lupinus angustifolius L. – 99, and Lupinus luteus L. – 95 days under conditions of the Steppe Crimea. The average yield of white lupin seeds was 1.63 t/ha; blue or narrow-leafed lupin – 1.18 t/ha; yellow lupin – 0.72 t/ha. L. albus is more productive compared to L. angustifolius and L. luteus. The seed yield of all studied varieties and samples of white lupin was higher than that of the standard one ‘Michurinsky’. Samples CH-2-17 and CH-78-16 were the most high-yielding (1.77 and 1.74 t/ha, respectively). Variety ‘Belorozovy 144’ was the most promising among the representatives of narrow-leafed lupin; its yield reached 1.64 t/ha. The best in grain size was white lupin; 1000-grains weight was within the range of 200-222g. Varieties of narrowleafed lupin ‘Belorozovy 144’ and ‘Bryansky kormovoy’ contained the least amount of alkaloids (0.021 and 0.022%, respectively).

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Feng, Lei, Xiufen Li, and Jian Chen. "Fouling Behavior of Extra-Cellular Polysaccharide Produced by Micrococcus luteus during Dead-End Microfiltration." In 2009 International Conference on Energy and Environment Technology. IEEE, 2009. http://dx.doi.org/10.1109/iceet.2009.550.

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Bounedjoum, Naila Bounedjoum, Attila Bodor, György Erik Vincze, Krisztián Laczi, Katalin Perei, and Gábor Rákhely. "Evaluation of Extracellular Organic Matter from Micrococcus luteus Effects on Hydrocarbon-Degrading Bacterial Communities." In The 6th World Congress on New Technologies. Avestia Publishing, 2020. http://dx.doi.org/10.11159/icepr20.148.

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Gaikwad, Shikha V., Esha S. Waghdhare, Priyanka S. Nawal, and Vikrant D. Gaikwad. "Isolation, screening of micrococcus luteus S2B1 bacteria and optimization of its biosurfactant producing conditions." In 2ND INTERNATIONAL CONFERENCE & EXPOSITION ON MECHANICAL, MATERIAL, AND MANUFACTURING TECHNOLOGY (ICE3MT 2022). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0183321.

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Bodor, Attila, Péter Petrovszki, György Erik Vincze, et al. "Extracellular Organic Matter from Micrococcus luteus Enhances the Bioconversion of Used Lubricants in Polluted Soil." In The 6th World Congress on New Technologies. Avestia Publishing, 2020. http://dx.doi.org/10.11159/icepr20.131.

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Topchiy, I., D. Amsheev, D. Stom, A. Kupchinsky, and M. Tolstoy. "Inhibition of Electricity Generation by Micrococcus Luteus 1-I in a Biofuel Cell by Respiratory Poisons." In I International Conference "Methods, models, technologies for sustainable development: agroclimatic projects and carbon neutrality", Kadyrov Chechen State University Chechen Republic, Grozny, st. Sher. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0011571200003524.

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Steen, Noelle, and Andrew L. Swindle. "COMPARING THE CAPACITY OF BACILLUS SUBTILIS, MICROCOCCUS LUTEUS, AND PSEUDOMONAS AERUGINOSA TO REDUCE CHROMIUM (VI) TO CHROMIUM (III)." In GSA 2020 Connects Online. Geological Society of America, 2020. http://dx.doi.org/10.1130/abs/2020am-357588.

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Licapa-Redolfo, Gladys S., Jhon Aguilar Reyna, Cristofer Oblitas Jauregui, and Shonel Cáceres Perez. "Innovations in Environmental Remediation: Use of Suillus Luteus in the Removal of Heavy Metals from Acid Mine Drainage." In 4th LACCEI International Multiconference on Entrepreneurship, Innovation and Regional Development (LEIRD 2024): "Creating solutions for a sustainable future: technology-based entrepreneurship". Latin American and Caribbean Consortium of Engineering Institutions, 2024. https://doi.org/10.18687/leird2024.1.1.378.

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OYMAK, Ahmet, and Arif PARMAKSIZ. "Determination of genetic variations of Carasobarbus luteus populations living in Euphrates River based on mitochondrial DNA cyt b sequences." In International Conference on Innovation in Bioinformatics and Biomedical Engineering. acavent, 2018. http://dx.doi.org/10.33422/icibbe.2018.12.11.

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Amsheev, Dmitry, Alexey Kashevskii, Devard Stom, Ivan Topchiy, and Alexander Safronov. "Research of the adhesion of the strain Micrococcus luteus 1-I on various electrode materials by electrochemical impedance spectroscopy." In INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “CURRENT ISSUES OF BIOLOGY, BREEDING, TECHNOLOGY AND PROCESSING OF AGRICULTURAL CROPS” (CIBTA2022) (To the 110th anniversary of V.S. Pustovoit All-Russian Research Institute of Oil Crops). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0140359.

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Reports on the topic "Luteus"

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วีรกุล, ปราจีน, สาโรช งามขำ, เกรียงศักดิ์ พูนสุข та จันทร์เพ็ญ สุวิมลธีระบุตร. อุบัติการเกิดมดลูกอักเสบในโคนม 30 วันหลังคลอดเนื่องจากเชื้อชนิดแบคทีเรียและผลตอบสนองต่อยาต้านจุลชีพ. จุฬาลงกรณ์มหาวิทยาลัย, 1995. https://doi.org/10.58837/chula.res.1995.34.

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การวิจัยครั้งนี้มีจุดมุ่งหมาย เพื่อตรวจหาเชื้อแบคทีเรียที่ทำให้เกิดมดลูกอักเสบของโคนม 30 วันหลังคลอด และทดสอบการตอบสนองของเชื้อแบคทีเรียที่พบต่อยาต้านจุลชีพของโคนม 67 ตัว ซึ่งมีเมือกจากช่องคลอดสีขุ่นผิดปกติจาก 17 ฟาร์มในเขตจังหวัดราชบุรี โดยเก็บตัวอย่างจากมดลูก ตรวจเพาะแยกเชื้อแบคทีเรียชนิดต้องใช้ออกซิเจน พบแบคทีเรีย 42 ชนิด ในโค 37 ตัว (55.2%) แบคทีเรียที่พบมาก ได้แก่ Staphylococcus aureus 28.2%, Corynebacterium pyogenes 23.1%, Acinetobacter calcoaceticus 10.3%, Escherichia coli 17.9%, ส่วนแบคทีเรียที่พบส่วนน้อย ได้แก่ Bacillus spp. 5.1% Streptococcus agalactiae 2.6%, Micrococcus luteus 2.6%, Bordetella spp. 2.6% Morexella urethralis 2.6% และ Pseudomonas spp. 2.6% ได้รายงานผลตอบสนองต่อยาต้านจุลชีพของแบคทีเรียที่ตรวจพบได้แก่ Gentamicin, Polymyxin-B, Ampicillin, Nitrofurantoin, Erythromycin, Colistin, Neomycin, Sulfamethoxazole – trimethoprim, Kanamycin, Tetracycline, Bacitracin, Penicillin G และ Streptomycin.

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James Biggs, Mary Mullen, and Kathryn Bennett. Development and Application of a Habitat Suitability Ranking Model for the New Mexico Meadow Jumping Mouse (Zapus hudsonius luteus). Office of Scientific and Technical Information (OSTI), 1999. http://dx.doi.org/10.2172/15132.

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Wolfenson, David, William W. Thatcher, and James E. Kinder. Regulation of LH Secretion in the Periovulatory Period as a Strategy to Enhance Ovarian Function and Fertility in Dairy and Beef Cows. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586458.bard.

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The general research objective was to increase herd pregnancy rates by enhancing corpus luteum (CL) function and optimizing follicle development, in order to increase conception rate and embryo survival. The specific objectives were: to determine the effect of the duration of the preovulatory LH surge on CL function; to determine the function of LH during the postovulatory period on CL development; to optimize CL differentiation and follicle development by means of a biodegradable GnRH implant; to test whether optimization of CL development and follicle dynamics in timed- insemination protocols would improve fertility in high-yielding dairy cows. Low fertility in cattle results in losses of hundreds of millions of dollars in the USA and Israel. Two major causes of low fertility are formation of a functionally impaired CL, and subsequent enhanced ovarian follicle development. A functionally impaired CL may result from suboptimal LH secretion. The two major causes of low fertility in dairy cattle in US and Israel are negative energy status and summer heat stress; in both situations, low fertility is associated with reductions in LH secretion and impaired development of the ovulatory follicle and of the CL. In Florida, the use of 450-mg deslorelin (GnRH analogue) implants to induce ovulation, under the Ovsynch protocol resulted in a higher pregnancy rates than use of 750-mg implants, and pregnancy losses tended to decrease compared to controls, due probably to decrease in follicular development and estradiol secretion at the time of conceptus signaling to maintain the CL. An alternative strategy to enhance progesterone concentrations involved induction of an accessory CL by injection of hCG on day 5 after the cows were inseminated. Treatment with hCG resulted in 86% of the cows having two CLs, compared with 23% of the control cows. Conception rates were higher among the hCG-treated cows than among the controls. Another approach was to replace the second injection of GnRH analogue, in a timed-insemination protocol, with estradiol cypionate (ECP) injected 24 h after the injection of PGF₂ₐ Pregnancy rates were comparable with those obtained under the regular Ovsynch (timed- AI) program. Use of ECP induced estrus, and cows inseminated at detected estrus are indeed more fertile than those not in estrus at the time of insemination. Collectively, the BARD-supported programs at the University of Florida have improved timed insemination programs. In Ohio, the importance of the frequency of LH episodes during the early stages of the estrous cycle of cattle, when the corpus luteum is developing, was studied in an in vivo experiment in which cows were subjected to various episodic exposures to exogenous bovine LH. Results indicate that the frequent LH episodes immediately following the time of ovulation are important in development of the corpus luteum, from the points of view of both size and functionality. In another study, rates of cell proliferation and numbers of endothelial cells were examined in vitro in CLs collected from cows that received post-ovulation pulsatile LH treatment at various frequencies. The results indicate that the corpora lutea growth that results from luteal cell proliferation is enhanced by the episodes of LH release that occur immediately after the time of ovulation in cattle. The results also show that luteal endothelial cell numbers did not differ among cows treated with different LH doses. In Israel. a longer duration of the preovulatory LH surge stimulated the steroidogenic capacity of granulosa-derived luteal cells, and might, thereby, contribute to a higher progesterone output from the bovine corpus luteum. In an in vivo study, a subgroup of high-yielding dairy cows with extended estrus to ovulation interval was identified. Associated with this extended interval were: low plasma progesterone and estradiol concentrations and a low preovulatory LH surge prior to ovulation, as well as low post- ovulation progesterone concentration. In experiments based on the above results, we found that injection of GnRH at the onset of estrus increased the LHpeak, prevented late ovulation, decreased the variability between cows and elicited high and uniform progesterone levels after ovulation. GnRH at estrus onset increased conception rates, especially in the summer, and among primiparous cows and those with low body condition. Another study compared ovarian functions in multiparous lactating cows with those in nulliparous non-lactating heifers. The results revealed differences in ovarian follicular dynamics, and in plasma concentrations of steroids and gonadotropins that may account for the differences in fertility between heifers and cows.

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Meidan, Rina, and Robert Milvae. Regulation of Bovine Corpus Luteum Function. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604935.bard.

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The main goal of this research plan was to elucidate regulatory mechanisms controlling the development, function of the bovine corpus luteum (CL). The CL contains two different sterodigenic cell types and therefore it was necessary to obtain pure cell population. A system was developed in which granulosa and theca interna cells, isolated from a preovulatory follicle, acquired characteristics typical of large (LL) and small (SL) luteal cells, respectively, as judged by several biochemical and morphological criteria. Experiments were conducted to determine the effects of granulosa cells removal on subsequent CL function, the results obtained support the concept that granulosa cells make a substaintial contribution to the output of progesterone by the cyclic CL but may have a limited role in determining the functional lifespan of the CL. This experimental model was also used to better understand the contribution of follicular granulosa cells to subsequent luteal SCC mRNA expression. The mitochondrial cytochrome side-chain cleavage enzyme (SCC), which converts cholesterol to pregnenolone, is the first and rate-limiting enzyme of the steroidogenic pathway. Experiments were conducted to characterize the gene expression of P450scc in bovine CL. Levels of P450scc mRNA were higher during mid-luteal phase than in either the early or late luteal phases. PGF 2a injection decreased luteal P450scc mRNA in a time-dependent manner; levels were significantly reduced by 2h after treatment. CLs obtained from heifers on day 8 of the estrous cycle which had granulosa cells removed had a 45% reduction in the levels of mRNA for SCC enzymes as well as a 78% reduction in the numbers of LL cells. To characterize SCC expression in each steroidogenic cell type we utilized pure cell populations. Upon luteinization, LL expressed 2-3 fold higher amounts of both SCC enzymes mRNAs than SL. Moreover, eight days after stimulant removal, LL retained their P4 production capacity, expressed P450scc mRNA and contained this protein. In our attempts to establish the in vitro luteinization model, we had to select the prevulatory and pre-gonadotropin surge follicles. The ratio of estradiol:P4 which is often used was unreliable since P4 levels are high in atretic follicles and also in preovulatory post-gonadotropin follicles. We have therefore examined whether oxytocin (OT) levels in follicular fluids could enhance our ability to correctly and easily define follicular status. Based on E2 and OT concentrations in follicular fluids we could more accurately identify follicles that are preovulatory and post gonadotropin surge. Next we studied OT biosynthesis in granulosa cells, cells which were incubated with forskolin contained stores of the precursor indicating that forskolin (which mimics gonadotropin action) is an effective stimulator of OT biosynthesis and release. While studying in vitro luteinization, we noticed that IGF-I induced effects were not identical to those induced by insulin despite the fact that megadoses of insulin were used. This was the first indication that the cells may secrete IGF binding protein(s) which regonize IGFs and not insulin. In a detailed study involving several techniques, we characterized the species of IGF binding proteins secreted by luteal cells. The effects of exogenous polyunsaturated fatty acids and arachidonic acid on the production of P4 and prostanoids by dispersed bovine luteal cells was examined. The addition of eicosapentaenoic acid and arachidonic acid resulted in a dose-dependent reduction in basal and LH-stimulated biosynthesis of P4 and PGI2 and an increase in production of PGF 2a and 5-HETE production. Indomethacin, an inhibitor of arachidonic acid metabolism via the production of 5-HETE was unaffected. Results of these experiments suggest that the inhibitory effect of arachidonic acid on the biosynthesis of luteal P4 is due to either a direct action of arachidonic acid, or its conversion to 5-HETE via the lipoxgenase pathway of metabolism. The detailed and important information gained by the two labs elucidated the mode of action of factors crucially important to the function of the bovine CL. The data indicate that follicular granulosa cells make a major contribution to numbers of large luteal cells, OT and basal P4 production, as well as the content of cytochrome P450 scc. Granulosa-derived large luteal cells have distinct features: when luteinized, the cell no longer possesses LH receptors, its cAMP response is diminished yet P4 synthesis is sustained. This may imply that maintenance of P4 (even in the absence of a Luteotropic signal) during critical periods such as pregnancy recognition, is dependent on the proper luteinization and function of the large luteal cell.

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Hagel, Stefan. ‘Coptic lutes’ scales – finding a robust approach. Verlag der Österreichischen Akademie der Wissenschaften, 2021. http://dx.doi.org/10.1553/oeai_ambh_4.

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Meidan, Rina, and Joy Pate. Roles of Endothelin 1 and Tumor Necrosis Factor-A in Determining Responsiveness of the Bovine Corpus Luteum to Prostaglandin F2a. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7695854.bard.

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The corpus luteum (CL) is a transient endocrine gland that has a vital role in the regulation of the estrous cycle, fertility and the maintenance of pregnancy. In the absence of appropriate support, such as occurs during maternal recognition of pregnancy, the CL will regress. Prostaglandin F2a (PGF) was first suggested as the physiological luteolysin in ruminants several decades ago. Yet, the cellular mechanisms by which PGF causes luteal regression remain poorly defined. In recent years it became evident that the process of luteal regression requires a close cooperation between steroidogenic, endothelial and immune cells, all resident cells of this gland. Changes in the population of these cells within the CL closely consort with the functional changes occurring during various stages of CL life span. The proposal aimed to gain a better understanding of the intra-ovarian regulation of luteolysis and focuses especially on the possible reasons causing the early CL (before day 5) to be refractory to the luteolytic actions of PGF. The specific aims of this proposal were to: determine if the refractoriness of the early CL to PGF is due to its inability to synthesize or respond to endothelin–1 (ET-1), determine the cellular localization of ET, PGF and tumor necrosis factor a (TNF a) receptors in early and mid luteal phases, determine the functional relationships among ET-1 and cytokines, and characterize the effects of PGF and ET-1 on prostaglandin production by luteal cell types. We found that in contrast to the mature CL, administration of PGF2a before day 5 of the bovine cycle failed to elevate ET-1, ETA receptors or to induce luteolysis. In fact, PGF₂ₐ prevented the upregulation of the ET-1 gene by ET-1 or TNFa in cultured luteal cells from day 4 CL. In addition, we reported that ECE-1 expression was elevated during the transitionof the CL from early to mid luteal phase and was accompanied by a significant rise in ET-1 peptide. This coincides with the time point at which the CL gains its responsiveness to PGF2a, suggesting that ability to synthesize ET-1 may be a prerequisite for luteolysis. We have shown that while ET-1 mRNA was exclusively localized to endothelial cells both in young and mature CL, ECE-1 was present in the endothelial cells and steroidogenic cells alike. We also found that the gene for TNF receptor I is only moderately affected by the cytokines tested, but that the gene for TNF receptor II is upregulated by ET-1 and PGF₂ₐ. However, these cytokines both increase expression of MCP-1, although TNFa is even more effective in this regard. In addition, we found that proteins involved in the transport and metabolism of PGF (PGT, PGDH, COX-2) change as the estrous cycle progresses, and could contribute to the refractoriness of young CL. The data obtained in this work illustrate ET-1 synthesis throughout the bovine cycle and provide a better understanding of the mechanisms regulating luteal regression and unravel reasons causing the CL to be refractory to PGF2a.

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Meidan, Rina, Jorge Flores, Keith Inskeep, and David Wolfenson. Controlling the bovine ovarian cycle by disrupting the endothelin system in corpora lutea and follicles with novel approaches: RNA interference (RNAi) and intra-luteal Atrigel implants. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695594.bard.

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In summary intensive studies carried out this year in both the US and Israel had established the methodology necessary for the achievement of the specific aims of the original proposal. Two complementary approaches to effectively neutralize the luteal ET- system were developed. In light of recent publications indicating that ET-2 might also have a physiological role in ovulation, the objectives of the original proposal have even more significant. Not only were the technologies to neutralize the luteal endothelin system developed in these studies, but additional important implications about the role of ET-1 were revealed. For example, direct early inhibitory effects of PGF2α were unmasked. It is possible that these early direct inhibitory effects could be related to functional aspects of luteal regression, while the effects observed after 12 hours of the PGF2α injection and that reversed by the ET receptor antagonist, could coincide with structural aspects of regression. Nevertheless, overall, the results clearly indicate that serum progesterone concentrations can effectively be elevated by the receptor antagonist which of great practical importance.

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Tarachiu, Alexandru. Lutein and some of its uses. ResearchHub Technologies, Inc., 2023. http://dx.doi.org/10.55277/researchhub.w6xi1bs1.

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Shemesh, Mordechai, and William Hansel. Hormone Production and Maintenance of the Corpus Luteum by the Bovine Blastocyst. United States Department of Agriculture, 1985. http://dx.doi.org/10.32747/1985.7562336.bard.

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Meidan, Rina, and Milo C. Wiltbank. Mechanisms involved in rescue of the corpus luteum during early pregnancy in lactating dairy cows. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600018.bard.

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