To see the other types of publications on this topic, follow the link: Luteus.
Dissertations / Theses on the topic 'Luteus'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Luteus.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Lauer, Iris. "Untersuchung der Strukturdynamik arbeitender F1-ATPase und ATP-Synthase aus Micrococcus luteus in Einzelschuss-Experimenten mit Synchrotronstrahlung." [S.l. : s.n.], 2001. http://ArchiMeD.uni-mainz.de/pub/2001/0127/diss.pdf.
Full text
2
OLIVEIRA, Louise Melo de Souza. "Atividades biológicas de metabólitos de cactaceae e Micrococcus luteus." Universidade Federal de Pernambuco, 2017. https://repositorio.ufpe.br/handle/123456789/26927.
Full textAbstract:
Submitted by Pedro Barros (pedro.silvabarros@ufpe.br) on 2018-09-13T21:32:55Z
No. of bitstreams: 2
license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)
TESE Louise Melo de Souza Oliveira.pdf: 1478143 bytes, checksum: 5477c219843e225280059e0100acaa49 (MD5)<br>Rejected by Alice Araujo (alice.caraujo@ufpe.br), reason: Por favor, atualizar com novo arquivo que está no banco.
Obrigada! on 2018-09-25T22:03:27Z (GMT)<br>Submitted by Pedro Barros (pedro.silvabarros@ufpe.br) on 2018-09-25T22:15:31Z
No. of bitstreams: 2
license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)
TESE Louise Melo de Souza Oliveira.pdf: 1697579 bytes, checksum: 37970b02f8a78c64db77c1f4f7dded6f (MD5)<br>Approved for entry into archive by Alice Araujo (alice.caraujo@ufpe.br) on 2018-09-25T22:18:05Z (GMT) No. of bitstreams: 2
license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)
TESE Louise Melo de Souza Oliveira.pdf: 1697579 bytes, checksum: 37970b02f8a78c64db77c1f4f7dded6f (MD5)<br>Made available in DSpace on 2018-09-25T22:18:05Z (GMT). No. of bitstreams: 2
license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)
TESE Louise Melo de Souza Oliveira.pdf: 1697579 bytes, checksum: 37970b02f8a78c64db77c1f4f7dded6f (MD5)
Previous issue date: 2017-05-29<br>FACEPE<br>Caatinga é um bioma com características distinta, encontrada apenas no território brasileiro, onde são encontradas muitas espécies endêmicas. A busca por novas moléculas biotivas é crescente, e os produtos naturais desempenham um papel significativo na procura por novos agentes terapêuticos. As Cactaceas que sobrevivem no ambiente da caatinga possuem adaptações anatômicas, fisiológicas e consequentemente modificações no metabolismo secundário. Micrococcus luteus é uma actinobactéria produtora do carotenoide amarelo, sarcinaxantina, esses pigmentos têm importantes funções biológicas, principalmente antioxidante. Neste contexto esse trabalho teve o objetivo de realizar uma revisão bibliográfica da família Cactaceae, analisar a atividade antimicrobiana e antioxidante de Cereus jamacaru, e identificar o pigmento carotenoíde de M. Luteus. Realizou-se um levantamento das espécies das cactaceas encontradas na região de Caatinga do Brasil, através de uma análise fitoquimica e farmacológica, evidenciando as principais atividades biológicas já descritas para essa família. Apesar do número reduzido de trabalhos observou-se que este grupo possui atividade antimicrobiana, antiviral, antiparasitária e antioxidante. Cereus jamacaru (mandacaru) é uma espécie pouco estudada do ponto de vista químico e farmacológico. Neste trabalho foi encontrado, em extrato metanólico, a presença de flavonoídes, fenilpropanoídes, monoterpenos, triterpeno e esteroides, possuindo atividade bactericida contra: Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, Streptococcus mitis, Escherichia coli e Staphylococcus aureus. Apresentou também atividade antioxidante pelo método DPPH (2,2- difenil-1-picril-hidrazila) e ABTS(2,2-azino-bis-(3-etil-benzotiazolina-6-ácido sulfônico). Duas cepas de M. luteus foram isolados do solo e identificadas por análises bioquímicas e moleculares. O pigmento foi caracterizado através de Cromatografia Líquida de Alta Eficiência (HPLC) e identificado como sarcinaxantina. Verificou-se também que este carotenoide possui atividade antioxidante, antimicrobiana e fator de proteção solar.<br>Caatinga is a biome with distinct characteristics, found only in Brazilian territory, and many endemic species are found. The search for new bioactive molecules is increasing, and natural products play a significant role in the search for new therapeutic agents. Cactaceae that survive in the caatinga environment have anatomical, physiological and consequent modifications in secondary metabolism. Micrococcus luteus is an actinobacterium producing the yellow carotenoid, sarcinaxanthin, these pigments have important biological functions, mainly antioxidant. In this context, the objective of this work was to carry out a bibliographic review of the Cactaceae family, to analyze the antimicrobial and antioxidant activity of Cereus jamacaru, and to identify the carotenid pigment of M. luteus. A survey of the species of cactaceae found in the Caatinga region of Brazil was carried out through a phytochemical and pharmacological analysis, evidencing the main biological activities already described for this family. Despite the small number of studies, it was observed that this group has antimicrobial, antiviral, antiparasitic and antioxidant activity. Cereus jamacaru (mandacaru) is a species little studied from the chemical and pharmacological point of view. In this work, the presence of flavonoids, phenylpropanoids, monoterpenes, triterpenes and steroids was found in methanolic extract, having bactericidal activity against: Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, Streptococcus mitis, Escherichia coli and Staphylococcus aureus. It also showed antioxidant activity by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) and ABTS (2,2-azino-bis- (3-ethyl-benzothiazoline-6-sulfonic acid) method). Two strains of M. luteus were isolated from the soil and identified by biochemical and molecular analyzes.The pigment was characterized by High Performance Liquid Chromatography (HPLC) and identified as sarcinaxanthin. This carotenoid was also found to have antioxidant, antimicrobial and sun protection factor.
3
Grechana, O. V., O. O. Saliy, A. G. Serbin, and L. A. Fukleva. "The pharmacognosy investigation of associated coumarins Lupinus luteus L." Thesis, Technical University in Zvolen, 2019. https://er.knutd.edu.ua/handle/123456789/15141.
Full textAbstract:
The genus Lupinus (Lupinus L.) is attributed to the legumes family (Fabaceae L.). About 1000 herbaceous, semi-bush and shrub, annual, winter and perennial lupines are described. In this paper we focuses on the issue of pharmacognostical study of plants is widely used in animal breeding, is the active substance in the manufacture of dietary supplements - the yellow lupine (Fabaceae). Harvested grass were analyzed after acid hydrolysis. Gas-liquid chromatography was first performed with mass spectrometric detection of hydrolysed raw material of Lupinus luteus L., where 56 compounds were found, of which 35 components were identified. After hydrolysis, the raw material of L. luteus L. contained a number of biologically active substances – fatty acids, alcohols, ketones etc. Two components from the class of true coumarins were identified: coumarin (16.4 mg%) and 6–methylcoumarin (2.7 mg%).
4
Florez, Roncancio Victor Julio. "Estudo do controle fotoperiodico e hormonal da floração em Solidago X luteus." [s.n.], 1997. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315342.
Full textAbstract:
Orientador: Maria de Fatima Domingos Aleixo Pereira<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-07-22T08:12:42Z (GMT). No. of bitstreams: 1
FlorezRoncancio_VictorJulio_D.pdf: 5014406 bytes, checksum: 8225825b1d38abb07d08b9566454e2b4 (MD5)
Previous issue date: 1997<br>Resumo: O efeito do fotoperíodo sobre o desenvolvimento vegetativo e a indução floral em Aster ericoides cv. Montecasino, Solidago chilensis M. e Solidago x luteus foi estudado em experimentos preliminares. Verificou-se que, de forma geral, os DL (20h) promoveram a indução floral e o aumento no número de ramificações laterais e de folhas, ao passo que, sob DC (8h) , as plantas de S. chilensis permaneceram em roseta, as de S. x luteus tiveram indução e antese floral, enquanto que em A. ericoides havia plantas em roseta e plantas induzidas. Em experimento sazonal com s. x luteus em diferentes fotoperíodos observou-se interação fotoperíodo-temperatura, pois, em fotoperíodos curtos (8h a 14h) e em NI prevaleceu o efeito da temperatura, permanecendo as plantas em roseta em "temperaturas baixas" (outono-inverno) e havendo indução floral em "temperaturas altas" (verão-outono), porém, com altura de caule inferior a 0,45m; ao passo que, em fotoperíodos longos(16h, 18h, 20h e LC), prevaleceu o efeito de indução floral do fotoperíodo, havendo alongamento caulinar próximo a O,90m mesmo em "temperaturas baixas". O tratamento de vernalização das estacas manteve as plantas em roseta em DC e retardou a indução floral nas plantas sob DL. Quando há "bolting" no verão-outono, nas plantas mantidas em fotoperíodos de 8h e NI, há um acúmulo de massa seca na raiz, semelhante ao da parte aérea. Em fotoperíodos, onde foi observada uma menor intensidade resposta indutiva de floração (8h, 10h, 12h, 14h e NI) e "temperatura baixa", ocorreu direcionamento preferencial de assimilados para a raiz. Nas melhores condições indutoras de floração (16h, 18h e 2Oh), que independem da temperatura, verificou-se direcionamento de fotoassimilados para a parte aérea. Como a indução floral ocorreu sob todos os fotoperíodos estudados, verificando-se diferenças quantitativas entre os diferentes fotoperíodos, o híbrido em estudo ficou caracterizado como uma PDL quantitativa, com fotoperíodo mais favorável para indução floral em torno de 16h; contudo, a antese floral foi antecipada em fotoperíodos curtos (8h, 10h e 12h) , refletindo também uma resposta quanti tativa no desenvolvimento floral. Não foi detectada atividade giberelínica em nenhum dos tratamento estudados. Foi constatada maior concentração de AIA em folhas e botões florais de plantas em DL em comparação aos DC, o que poderia estar relacionado à maior velocidade de antese floral em plantas sob DC. As maiores concentrações de ABA, em relação ao início dostratamentos fotoperiódicos ocorreram em folhas e botões florais de plantas em DC; assim, no balanço AIA:ABA, estes estariam em concentrações opostas, principalmente no botão floral em DC, onde se observou a maior quantidade de ABA e a ausência de AIA. A atividade citocinínica, detectada nos extratos provenientes de folhas de plantas sob DC e de botões florais de plantas no início dos tratamentos, sob DL e DC, associado ao fato de o tratamento com KI, em botões florais de plantas em DL, ter acelerado a antese floral, foram as evidências de que citocininas endógenas estariam envolvidas no processo; porém, entre as quatro citocininas dosadas (Z, ZR, iP e iPA) somente o iP e iPA foram detectados. Uma diminuição significativa de iP em folhas de plantas sob DC pode estar sendo estimulada por uma taxa de transporte mais ativa na forma de iPA para o botão floral, o que, por sua vez, estimularia a maior velocidade de antese floral observada sob DC<br>Abstract: The effect of photoperiod on vegetative development and floral induction in Aster ericoides cv. Montecasino, Solidago chilensis M. and Solidago x luteus was studied in the preliminary experiments. Floral induction was generally promoted, and the number of lateral branches and leaves increased under long days (20h). The S. chilensis plants remained rosettes, while in S. x luteus floral induction occurred under short days (8h). However, A. ericoides showed both responses, rosettes and floral induction. In seasonal experiments with different lengths of photoperiods the habit of S. x luteus changed as a result of interaction photoperiod-temperature. In plants under short photoperiods (8h to 14h) and night break the temperature effect prevailed: the plants remained rosettes in the autumn-winter period, and the bolting was promoted by the summer-autumn temperatures with stem elongation not superior to O.45m. In the longer photoperiods (16h, 18h, 20h and continuous light) the photoperiodic effect prevailed, promoting floral induction with stem elongation near to O.90m even in the autumn-winter temperatures. The vernalization of cuttings kept the plants as rosettes under 8h, and delayed the floral induction in plants under 18h. In plants in which bolting was promoted in summer-autumn temperatures, under photoperiods of 8h and night break, root and shoot partitioning of dry matter occurred. Autumn-winter season and photoperiods with less intensive induction response (8h, 10h, 12h, 14h and night break) resulted in accumulation of dry matter in the roots, while in both seasons, autumn-winter and summer-autumn, shoot diversion of assimilates was found under longer photoperiods (16h, 18h and 20h). As floral induction occurred under all photoperiods, but with a more rapid and intense response being recorded under photoperiods next to 16h, this hybrid was characterized as a quantitative long-day plant, although the rate of flower bud development to anthesis was more rapid in the shorter photoperiods (8h, 10h, 12h). In any treatment, the activity of gibberellin-like substances was not detected. In long-day (18h) plants, it was observed a higher concentration of AIA in leaves and floral buds than in short-day (8h) plants, suggesting that it may be related to a more rapid anthesis in plants kept in short days. In extracts from leaves and flower buds in short-day plants the higher levels of ABA were found in relation to the beginning of the photoperiodic treatments. Therefore, it seems to exist an indirect relation between the levels of AIA and ABA mainly in the flower buds under 8h, in which the higher amount of ABA is coincident to disappearance of AIA. Substances with cytokinin activity were detected in leaves of plants kept in short days, and in flower buds of plants at the beginning of the photoperiodic treatments, and plants kept in long days and in short days, along with the fact that the kinetin treatment accelerated the development of anthesis of flower buds of plants under long days, point to a possible role of the endogenous cytokinins in the process. The levels of zeatin-type (Z, ZR) and isopentenyladenosine-type (iP, iPA) cytokinins were examined but only iP and iPA were detected. The decrease in the iP content of plants kept in short days could be the consequence of a more active transport rate of iPA, speeding up the flower bud anthesis in the plants under short days<br>Doutorado<br>Doutor em Ciências Biológicas
5
Ruiz, Posadas Lucero del Mar. "Rhizospheric calcium and stomatal behaviour in Lupinus luteus L. and Commelina communis L." Thesis, Lancaster University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296972.
Full text
6
PIERRE-HEBERT, JOSIANE. "Etapes initiales de la reparation de l'adn modifie par alkylation chez micrococcus luteus." Paris 6, 1986. http://www.theses.fr/1986PA066652.
Full text
7
Borowska, Magdalena. "Wpływ wybranych regulatorów wzrostu i Ekolistu na plonowanie łubinu żółtego (Lupinus luteus L.)." Rozprawa doktorska, [Nakł.aut.], 2001. http://dlibra.utp.edu.pl/Content/341.
Full textAbstract:
Badania własne miały określić reakcję łubinu żółtego na regulatory wzrostu oraz mikro - i makroskładniki zastosowane w formie oprysku na początku kwitnienia w celu ograniczenia zjawiska opadania organów generatywnych, a tym samym zwiększenia produkcyjności roślin, wartości pokarmowej nasion, ich żywotności i wigoru. Przedmiotem badań była odmiana łubinu żółtego "Teo"
8
Spit, Anthony. "The primary structures and the evolutionary consequences of the olisthodiscus luteus histone proteins." Doctoral thesis, University of Cape Town, 1993. http://hdl.handle.net/11427/21498.
Full textAbstract:
Bibliography: pages 187-194.<br>During the course of this study, the histones of the algae Olisthodiscus luteus were isolated, purified and fractionated. Identification of the histones was achieved by partial primary structure analysis. The histones Hl, H2A, H2B, H3 and H4 were found to be present in the O. luteus nucleus. The complete structure of H2A and H4 was determined. There is no evidence of the existence of the unique histone HO1 (Rizzo et al., 1985). Construction of phylogenetic trees suggests that the alga Olisthodiscus luteus diverged from the animal line. By sequence comparison, the most closely related histone sequence to the algae was found to be that of the echinodermata. An endosymbiotic event between an echinodermata ancestor and a primitive unicellular alga is hypothesised in an attempt to explain the smilarity between the histones.
9
Whitcombe, David Mark. "Molecular cloning and analysis of a β-1,3-glucanase from Arthrobacter luteus (Oerskovia xanthineolytica)". Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/35179.
Full textAbstract:
Species of Arthrobacter luteus, also known as Oerskovia xanthineolytica, can utilise yeast cells as a growth substrate. This unusual ability is due to the secretion of a battery of hydrolytic enzymes which degrade the yeast cell wall and thus lyse the cells. Although many hydrolytic enzymes are important in the degradation of the yeast cell wall, the key activities are endo beta--l,3-glucanases. In order to characterise components of the yeast lytic system and the genetic organisation of this little-understood organism, a molecular cloning approach was adopted. Large clones expressing beta-1,3-glucanase were isolated from a library of A. luteus DNA constructed in the positive selection vector pKGW. By a combination of subcloning, restriction mapping and Southern analysis, it was determined that the clones contained virtually the same inserts. Additional subcloning, transposon mutagenesis, deletion mapping and nucleotide sequencing were used to identify at least one glucanase gene. The predicted protein product had a molecular weight of about 46 kD. When the gene was expressed in a number of in vivo and vitro systems including E. coli minicells and a Streptomyces coupled transcription/translation system, the protein observed had a similar molecular weight. Furthermore, when the protein was produced in E. coli and run on activity stained gels, the beta-glucanase activity co-migrated with the major glucanase of A. luteus. In addition the E. coli-produced glucanase had the ability to cause limited lysis of yeast.
10
Prusiński, Janusz. "Biologiczne i agrotechniczne uwarunkowania rozwoju i plonowania zróżnicowanych genotypów łubinu żółtego (Lupinus luteus L.)." Rozprawa habilitacyjna, Wydaw. Uczelniane Akademii Techniczno-Rolniczej, 1995. http://dlibra.utp.edu.pl/Content/486.
Full text
11
Lança, Augusto Joaquim de Carvalho. "Estudo do valor alimentar da tremocilha brava (Lupinus luteus L.), em ovinos, e do seu efeito sobre a composição química do leite e qualidade do queijo de Serpa." Doctoral thesis, Universidade de Évora, 1999. http://hdl.handle.net/10174/11639.
Full textAbstract:
O objectivo deste trabalho foi averiguar se o sabor amargo e as propriedades anti-microbianas dos alcalóides da tremocilha brava afectavam a ingestão e moção digestiva da dieta, a composição química do leite e a qualidade do queijo de ovelha. Numa P experiência comparou-se um suplemento de tremocilha brava (TB) (1,0% alcalõides) com suplementos constituidos por variedades doces de tremoço (T) e tremoço-de-folhas-estreitas (TFE), e com um suplemento testemunha de bagaço de soja (BS). Esta suplementação foi fornecida aos animais com feno de baixa qualidade e de modo a manter as dietas isoazotadas. Quer a ingestão de MS total (g/KgPV0.75) quer a DNDF e DPB das 4 dietas não diferiram significativamente entre si. A DMO da dieta TB (72,0%), não diferiu significativamente (P>0,05) das apresentadas pelas dietas BS (70,5%) e T (74,2%) mas foi inferior à da dieta TFE (77,5%), a que continha maior proporção de concentrado. Também a DADF da dieta TB (66,4%) não diferiu significativamente das determinadas nas dietas BS (61,0%) e T (69,6%) mas foi inferior à da dieta TFE (74,4%). Tanto os valores do balanço azotado de cada dieta como os coeficientes de digestibilidade dos suplementos não mostraram diferenças significativas.
Numa 2ª experiência estudou-se o efeito dos alcalóides da TB sobre a actividade microbiana do líquido ruminai ovino incubado in vitro. Num primeiro conjunto de incubações submeteu-se o líquido ruminal a 6 concentrações crescentes de esparteína (0, 1, 2, 3, 4 e 5 mM). Ao fim de 24h de incubação, os líquidos ruminais sem esparteína produziram significativamente mais gás (603 ml) (P<0,05) do que aqueles em que a concentração do alcalóide foi de 2 mM (578 ml), 3 mM (570 ml), 4 mM (573 ml) e 5 mM (567 ml). Também às 6 e 12 h de incubação se assistiu a um declínio da produção de gás conforme aumentou a concentração de esparteína (P<0,05), que foi no entanto mais nítido às 6 h, com as máximas concentrações a registarem apenas -61% do gás produzido nos fermentadores testemunha (P<0,05). A concentração de N-amoniacal só apresentou valores significativamente diferentes (P4,05) às 12 h da fermentação, com o líquido ruminai sem esparteína a exibir uma concentração de N-amoniacal de 34,2 mg/100m1, inferior ao teor de 37,0 mg/100 ml dos fermentadores com 1 e 3 mM de esparteína e 37,9 e 38,0 mg/100m1 naqueles com 4 e 5 mM. Os valores do pH do líquido ruminai livre de esparteína foram sempre inferiores àqueles registados nas outras concentrações, mas só às 6 e 12 h e para as duas concentrações máximas estas diferenças foram significativas (P<0,05). 0 pH registado nos fermentadores correspondentres às situações extremas (0 e 5 mM) foram, respectivamente, de 6,86 e 7,20 às 6 h e de 6,17 e 6,40 às 12 h. Noutro conjunto de incubações, comparou-se a vitalidade do líquido ruminai quando incubado com tremocilha.brava ou com a mesma quantidade de tremocilha doce Topaz aos níveis de 5,10 e 15 g de MS. Em qualquer das situações não se obtiveram produções de gás significativamente diferentes, à excepção do nível de 5 g às 12 h em que a TB originou mesmo uma maior produção de gás (P<0,05). Ao ,cabo de 12 e 24 h de incubação registaram-se também diferenças sicativas (P<0,05) nos valores do pH, com os fermentadores com TB a apresentarem um pH inferior aos da tremocilha doce. ##### ABSTRACT - The objective of this work was to determine if the bitter taste and the anti-microbial properties of bitter yellow lupin alkaloids affected the intake and digestive utilization of the diet, the chemical composition of milk and the quality of ewe cheese. In a 1st experiment, a bitter yellow lupin supplement (BYL) (1.0 % alkaloids) was compared to supplements constituted by sweet varieties of white lupin (WL), narrow leafed lupin (NLL) and by a control supplement of soybean meal (SBM). This supplementation was given to animals along with poor quality hay in order to mantain the isonitrogen level in the diets. Total dry matter (DM) intake (g/KGLW0,75) as well as NDF and CP digestibilitiesfrom the four diets were not significantly different. The organic matter (OM) digestibility from de BYL diet (72.0%) was not significantly different (P>0.05) from those presented by SBM (70.5%) and WL diets (74,2%), but was lower than that presented by the NLL diet (77.5%). This last diet had the greatest proportion of concentrate. In the same way, ADF digestibility of the BYL diet (66,4%) did not differ significantly from the digestibility of SBM (61.0%) and WL (69.6%) diets, although it was lower than the digestibility of the NLL diet (74.4%). Both the nitrogen balance values from each diet and the digestibility coefficients for supplements showed no significant differences.
In a 2nd experiment, the effect of BYL alkaloids on the microbial activity of in vitro incubated sheep rumen fluid was studied. In a first set of incubations, rumenfluid was submitted to 6 increasing sparteine concentrations (0, 1, 2, 3, 4 and 5 mM). After 24h of incubation, sparteine-free rumen fluids produced significantly more gas (603 mi) (P<0.05) than those in wich alkaloid concentration was 2 mM (578 ml), 3 mM (570 ml), 4 mm (573 ml) and 5 mM (567 m1). Between the 6th and 12th hour of incubation, a decrease was observed in the production of gas when the concentration of sparteine increased (P<0.05). However, this decrease was more cleariy seen at 6 hours when maximum concentrations registered only 61% of the gas produced in the control fermentators (P<0.05). The concentration of ammoniated N only presented significantly different values (P<0.05) at 12 hours of fermentation, while the sparteine-free rumen fluid presented an ammoniated N concentration of 34.2 mg/100 ml. This value was lower than the 37.0 mg/100m1 value recorded in fermentators with 1 and 3 mM of sparteine and 37.9 and 38.0 mg/100m1 in those with 4 and 5 mM. The pH values of sparteine free rumen fluid were always lower than those recorded in other concentrations. However, differences were only significant (P<0.05) for the two maximum concentrations at 6 and 12 hours. The pH recorded in extreme situation fermentators (0 and 5 mM) was 6.86 and 7.20 at 6 hours and 6.17 and 6.40 at 12 hours, respectively. The vitality of rumen fluid when incubated with BYL or with the same quantity of Topaz sweet yellow lupin at 5, 10 and 15 g DM will be compared in another group of incubations. The production of gas was not significantly different in any of these cases, except for the 5 g level at 12 hours, in wich BYL led to a greater production of gas (P<0.05). After 12 and 24 hours of incubation, significant differences (P<0.05) were recorded in pH values, while BYL fermentators presented a lower pH than those with sweet lupin.
The effect of sheep rumen flora on in vitro BYL alkaloids was studied in a 3rd experiment. Small quantities of lupin (0.5g DM) were incubated in rumen fluid and the concentrations of lupanine, sparteine, gramine and lupanine were measured at the beginning of fermentations and 24 and 48 hours. The concentration of lupinine, the predominant alkaloid, did not show any significant change (P>0.05), presenting 65.5 pg/ml at 0 hours and 67.3 pg/ml at 48 hours. The concentrations of the other alkaloids changed significantly during fermentation.
12
Pellon, Gérard. "Le système de pontage du peptidoglycane de Micrococcus luteus : DD-peptidases et récepteurs β-lactamiques". Lyon 1, 1986. http://www.theses.fr/1986LYO19006.
Full text
13
Pellon, Gérard. "Le Système de pontage du peptidoglycane de Micrococcus luteus DD-peptidases et récepteurs ß-lactamiques /." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb376003575.
Full text
14
Nogueira, Elaine. "Estudo da relação estrutura-atividades e de propriedades do Hb40-61a, uma hemocidina sintética." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-07022008-145303/.
Full textAbstract:
A hemoglobina (Hb) é uma fonte reconhecida de peptídeos com funções biológicas diversas. O fragmento 33-61 da cadeia α da Hb, isolado do trato gastrointestinal do carrapato Boophilus microplus, foi o primeiro a ser descrito com ação antimicrobiana. O seu análogo sintético amidado, Hb33-61a, mostrou-se ativo contra bactérias Gram-positivas e fungos [Fogaça et al. (1999) J. Biol. Chem. 274, 25330-4]. O estudo de análogos do Hb33-61 nas formas amidada e com carboxila livre revelou que a amidação provoca aumento significativo da atividade frente a Candida albicans. Por apresentar propriedades biológicas e estruturais idênticas às do Hb33-61a, o Hb40-61a pareceu ser a sua porção mÌnima ativa [Sforça et al. (2005) Biochemistry 44, 6440- 51; Machado et al. (2007) Biopolymers 88, 413-26]. Para comprovar tal sugestão, no presente trabalho, sintetizamos, purificamos e caracterizamos novos an·logos do Hb33-61a, bem como os avaliamos quanto às suas atividades frente a C. albicans e Micrococcus luteus. Os resultados confirmaram a sugestão apenas para a ação antifúngica. O análogo Hb40-61a também se mostrou ativo frente a C. albicans resistente a fluconazol. A sua atividade antifúngica se mostrou fortemente dependente da força iônica do meio. A sua baixa atividade hemolítica foi confirmada mesmo em meio de baixa força iônica. O peptídeo Hb40-61a não apresentou sinergismo com o fluconazol frente a C. albicans. A cinética de morte celular mostrou que ele mata a levedura de forma rápida. Portanto, esta hemocidina sintética pode apresentar valor comercial se a via de administração for tópica ou se o seu uso envolver meios de baixa força iônica. Além disso, ela é um modelo valioso para o estudo do mecanismo de ação de peptídeos antimicrobianos com características estruturais similares e pode servir de base para o desenho de novos agentes antibiôticos.<br>It is well known that hemoglobin (Hb) is a source of biologically active peptides. The fragment 33-61 of bovine hemoglobin α-chain, isolated from the gut contents of the tick Boophilus microplus, was the first identified with antimicrobial activity . Its amidated analogue, Hb33-61a, showed to be active against Gram-positive bacteria and fungi strains [FogaÁa et al. (1999) J. Biol. Chem. 274, 25330-4]. The study of a series of carboxyl-free and amidated synthetic analogues of Hb33-61 revealed that C-terminus amidation enhances the activity against Candida albicans. Since Hb33-61a and Hb40-61a presented identical biological and structural properties, it seemed that Hb40-61a was Hb33-61a minimal active motif [SforÁa et al. (2005) Biochemistry 44, 6440- To test this suggestion, in the present study 51; Machado et al. (2007) Biopolymers 88, 413-26]. we synthesized, purified and characterized Hb40-61a analogues and assayed them against C. albicans and Micrococcus luteus. The results confirmed the suggestion only for the antifungal activity. When tested against fluconazole-resistant C. albicans, Hb40-61a was also active. Its antifungal activity showed to be dependent on the ionic strength of the medium. Its low hemolytic activity was confirmed even under low ionic strength conditions. Hb40-61a had no synergic effect with fluconazole on C. albicans. In vitro time-kill assays demonstrated that Hb40-61a kills the yeast rapidly. Therefore, this synthetic hemocidin may be of commercial interest for topical application or other uses involving low ionic strength medium. Moreover, it can serve as a template for the study of the mechanism of action of structurally related antimicrobial peptides or for the design of novel antibiotic drugs.
15
Pohl, Pohl Nélida Beatriz Mercedes. "Importancia de la guía de néctar en la interacción entre herbivoría floral y polinización en Mimulus Luteus." Tesis, Universidad de Chile, 2003. http://www.repositorio.uchile.cl/handle/2250/106703.
Full textAbstract:
Magíster en Ciencias Biológicas con mención en Ecología y Biología Evolutiva<br>Mimulus luteus
es una planta herbácea cuyas flores son visitadas por insectos y por el picaflor
cordillerano,
Oreotrochilus leucopleurus
. Presenta una guía de néctar que puede ser blanco de
herbivoría floral. En el presente trabajo se analiza la relación entre herbivoría de señales y
polinización en
M. luteus
, relación que no ha sido abordada en los escasos estudios que asocian
herbivoría y polinización, y sus efectos sobre los caracteres vegetales.
Una serie de cruzamientos experimentales demostraron que
M. luteus
requiere un vector de
polen para asegurar su reproducción. La herbivoría natural sobre la guía de néctar disminuyó la
producción de semillas de
M. luteus.
Al evaluar la producción de semillas en un experimento
factorial de 2 X 2 con exclusión de picaflores y daño artificial como factores principales, se
observó que el efecto del daño es contingente a la presencia de los picaflores. Las flores dañadas
artificialmente y que no excluyen a los picaflores presentaron una menor producción de semillas,
lo que indica que tales visitantes florales discriminan negativamente las flores con la guía de
néctar dañada. Se sugiere que esta discriminación se debería a una preferencia aprendida por los
fenotipos florales de mayor tamaño de guía de néctar, señal que los picaflores asociarían a una
mayor recompensa.
Se concluye que la guía de néctar afecta no sólo la polinización de
M. luteus,
sino también la
interacción entre herbivoría floral y polinización, a través de su efecto sobre la atracción al
polinizador
O. leucopleurus.
16
ESNAULT, BLANCHARD MARIE-ANDREE. "Les protéines de réserve des graines de lupin jaune (lupinus luteus l. Légumineuses) : purification, composition et biosynthèse." Rennes 1, 1994. http://www.theses.fr/1994REN10028.
Full textAbstract:
Les globulines du lupin jaune sont constituees de quatre types de proteines. Les plus representees quantitativement, les conglutines alpha et beta, appartiennent aux classes des legumines et vicilines. La conglutine alpha est formee de polypeptides acides associes par des liaisons disulfures a des polypeptides basiques. Deux sortes d'associations sont mises en evidence. Elles permettent de proposer un schema de maturation du pro-polypeptide precurseur. A l'oppose des autres legumineuses, les legumines du lupin jaune sont synthetisees avant les vicilines. Ces dernieres ne sont matures que tres tardivement, avant la dessiccation de la graine. Les deux autres proteines de reserve des lupins sont les conglutines delta et gamma. La premiere est complexe dans le lupin jaune. Toutefois, la purification de la proteine dans d'autres especes de lupins met en evidence un modele commun. La conglutine gamma, simple sur le plan de sa structure a un role encore inconnu dans la graine. Ses differentes caracteristiques sont discutees dans ce sens, et notamment, sa biosynthese precoce et son elution dans l'eau d'imbibition des graines
17
Barron, Vincent N. (Vincent Neal). "Comparison of Aspartate Transcarbamoylase and Pyrimidine Salvage in Sporosarcina urea, Sprolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus." Thesis, University of North Texas, 1994. https://digital.library.unt.edu/ark:/67531/metadc278938/.
Full textAbstract:
The enzyme that catalyzes the committed step in pyrimidine biosynthesis, aspartate transcarbamoylase, has been compared in selected endospore-forming organisms and in morphologically similar control organisms. The ATCases and pyrimidine salvage from Sporosarcina ureae, Sporolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus were compared to those of Bacillus subtilis. While the ATCases from Sporosarcina ureae, Sporolactobacillus inulinus, and L. fermentum were found to exhibit characteristics to that of Bacillus with respect to molecular weight and kinetics, M. luteus ATCase was larger at approximately 480 kDa. Furthermore, pyrimidine salvage in Sporosarcina ureae and M. luteus was identical to those of B. subtilis, while pyrimidine salvage of Sporolactobacillus inulinus and L. fermentum resembled that of the pseudomonads.
18
Oliveira, Cleber Barbosa de [UNESP]. "Avaliação das células luteais de fêmeas taurinas (Bos taurus taurus) e zebuínas (Bos taurus indicus)." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/105940.
Full textAbstract:
Made available in DSpace on 2014-06-11T19:35:11Z (GMT). No. of bitstreams: 0
Previous issue date: 2007-03-30Bitstream added on 2014-06-13T20:06:40Z : No. of bitstreams: 1
oliveira_cb_dr_jabo.pdf: 442407 bytes, checksum: be6648eb5ab8b4cba1b22c5554dd2b9d (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>O objetivo deste trabalho foi determinar o número de células luteais bovinas, comparando fêmeas taurinas com zebuínas no início e final do ciclo estral. Foram coletados corpos lúteos de 16 fêmeas sendo 8 taurinas e 8 zebuínas, distribuídas em 4 grupos sendo coletados os ovários nos dias 3 a 5 (2 grupos: taurino e zebuíno) e 16 a 18 (2 grupos: taurino e zebuíno) do ciclo estral. Os corpos lúteos foram processados para microscopia óptica e avaliouse as células luteais pequenas, grandes e intermediárias, quanto ao número celular, diâmetro, área e perímetro. Os animais taurinos apresentaram maior quantidade de células luteais pequenas que os zebuínos no início do ciclo estral (p<O,05) e final do ciclo estral (p<O,05). Registrou-se diferença nos valores médios do diâmetro, perímetro e área das células luteais grandes, pequenas e intermediárias entre animais taurinos e zebuínos, tanto no início quanto no final do ciclo estral.<br>The aim of this work was to determine the number of bovine luteal cells comparing 80S taurus females with 80S indicus females at the beginning and at the end of estrous cycle. Sixteen corpus luteum were collected in eight 80S taurus cattle and in eight 80S indicus cattle, distributed into four groups. The ovaries were collected from the third and the fifth days (two groups: 80S taurus females and 80S indicus females) and from the sixteenth and the eighteenth days (two groups: 80S taurus caUle and 80S indicus cattle) of the estrous cycle. The corpus luteum was processed to optical microscopy and the small, big and intermediate-sized luteal cells were evaluated considering the number of cells, diameter, area and perimeter. The 80S taurus females presented a bigger amount of smallluteal cells than the 80S indicus females at the beginning of the estrous cycle (p,O,05) and at the end of the estrous cycle (p<O,05). The difference in the average values of the diameter, perimeter and the area of big, small and intermediate-sized luteal cells have been registered among 80S taurus cattle and 80S indicus cattle, at the beginning and also at the end of the estrous cycle.
19
Closson, Maude Marie Morais. "Estudo do efeito da substituição do bagaço de soja por tremoço branco (Lupinus Albus cv. Estoril) e por tremocilha (Lupinus Luteus cv. Mister) na alimentação de coelhos." Master's thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, 2019. http://hdl.handle.net/10400.5/18040.
Full textAbstract:
Dissertação de Mestrado em Engenharia Zootécnica – Produção Animal<br>O trabalho experimental apresentado teve como objetivo estudar o efeito da inclusão do tremoço branco e da tremocilha, como substitutos integrais ao bagaço de soja na alimentação de coelhos. Estudou-se o efeito nas performances, na digestibilidade e na avaliação das características pós-morte. Utilizaram-se sessenta coelhos, distribuídos de forma uniforme pelos três regimes diferentes: bagaço de soja (C), tremoço branco (TMC) e tremocilha (TLH).
Observou-se um aumento de peso diário dos coelhos de TLH inferior (42,1g/dia) ao de C e o índice de conversão pior nas últimas três semanas de ensaio (P<0,05). A digestibilidade da MO de C foi significativamente superior (62,2%) ao de TMC (59,6%), já a digestibilidade da PB e da GB de TMC foi significativamente inferior (71,4% e 81,6% respectivamente) ao registado em TLH (73,8% e 86,3% respectivamente).
A concentração de propionato no conteúdo cecal de C foi significativamente superior a TLH e o pH cecal inferior (P<0,05). O pool AGV de C foi superior ao de TMC (P <0,05). Não houve diferenças na ingestão média diária, no desenvolvimento do aparelho digestivo e nas características pós-morte. A incorporação de Lupinus ao nível testado (15%) prejudicou ligeiramente as performances. Estudar um nível de incorporação inferior poderá mostrar-se interessante.<br>ABSTRACT - Study of the effect of soybean meal substitution on white lupine ("Lupinus Albus" cv. Estoril) and for yellow lupine ("Lupinus Luteus" cv. "Mister") on rabbit feeding - This experimental work presented had as objective to study the effect of the inclusion of white lupine and yellow lupine as integral substitutes of soybean meal in the feeding of rabbits. It has been studied the effect in performance, digestibility and evaluation of post-mortem characteristics. Sixty rabbits were used and uniformly distributed by three different regimes: soybean meal (C), white lupine (TMC) and yellow lupine (TLH).
It was observed that the daily weight increase of TLH (42,1 g/day) was lower at C and conversion index worse in the last three weeks of testing (P <0,05). The digestibility of MO in C was significantly higher (62,2%) than TMC (59,6%), and the digestibility of CP and GB in TMC was significantly lower (71,4% and 81,6%, respectively) to TLH (73,8% and 86,3%, respectively).
The concentration of propionate in the caecal content of C was significantly higher than TLH and caecal pH was lower (P <0,05). The pool AGV of C was higher than TMC (P <0,05). There were no differences in mean daily intake, digestive tract development and postmortem characteristics. The incorporation of Lupinus at the level tested (15%) slightly affected performances. Studying a lower level of incorporation may prove to be interesting.<br>N/A
20
Li, Dora. "The Ncm-1 gene for resistance to Cucumber mosaic virus in yellow lupin (Lupinus luteus): molecular studies and marker development." Thesis, Li, Dora (2012) The Ncm-1 gene for resistance to Cucumber mosaic virus in yellow lupin (Lupinus luteus): molecular studies and marker development. PhD thesis, Murdoch University, 2012. https://researchrepository.murdoch.edu.au/id/eprint/10623/.
Full textAbstract:
Cucumber mosaic virus (CMV) is an important virus pathogen of lupins in Australia which causes serious yield losses of up to 60% in epidemic years. In commercially grown lupin (Lupinus angustifolius and L. luteus) crops CMV is spread non-persistently by aphid vectors, but it can also be seed borne and this extends virus infection into successive generations. Resistance to CMV has been identified in L. luteus cv. Wodjil and is the conferred by the Ncm-1 gene. The aims of this research were to study the Ncm-1 gene in order to gain a better understanding of resistance in yellow lupin, and to develop a molecular marker linked to Ncm-1 for use in marker assisted selection.
Previously published data by Jones et al (1996) identified Ncm-1 as being a single dominant resistance gene, however, phenotypic analysis of CMV infection in a segregating L. luteus mapping population in this thesis was consistent with the Ncm-1 gene being a dominant gene modified by at least one other minor gene. The polygenic nature of CMV resistance in this genetic background was further supported by AFLP analysis which identified one major and one minor QTL associated with resistance.
A PCR based approach, using degenerate primers designed on conserved disease resistance protein motifs, was used to identify resistance gene analogues (RGA) in L. luteus. Comparative analysis revealed that RGAs isolated from L. luteus were members of the TIR-NBS-LRR class of R proteins and were similar to the TMV resistance gene N identified in tobacco and the RT4-4 CMV resistance gene from pepper. Extensive comparative analysis using the genomes of model species (including Medicago truncatula, Glycine max, Arabidopsis thaliana and Lotus japonicus) was explored and validated the assignment from L. luteus RGAs to the category of candidate gene for CMV resistance. The RGAs identified in L. luteus were found to be highly conserved in both the CMV resistant and susceptible varieties tested. SNPs which resulted in non-synonymous mutations were identified using cDNA based 5’ RACE and used to develop a single nucleotide primer extension (SNuPE) assays for MALDI-ToF mass spectrophotometric analysis. As SNuPE is based on the allele specific extension of a single nucleotide, genotyping is highly accurate and provides co-dominant information. Two SNuPE assays were developed based on the RGAs isolated and validated on bulked samples from two L.luteus populations segregating for CMV resistance. One assay, SNuPE A267→C was found to associate with CMV resistance. This co-dominant assay is the first of its kind reported for yellow lupin.
21
Sen, Aritro. "Cellular mechanisms of luteal regression in the bovine corpus luteum (CL)." Morgantown, W. Va. : [West Virginia University Libraries], 2005. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4298.
Full textAbstract:
Thesis (Ph. D.)--West Virginia University, 2005.<br>Title from document title page. Document formatted into pages; contains xvi, 155 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 103-152).
22
Bramley, Helen. "Water flow in the roots of three crop species : the influence of root structure, aquaporin activity and waterlogging." University of Western Australia. School of Plant Biology, 2006. http://theses.library.uwa.edu.au/adt-WU2006.0102.
Full textAbstract:
[Truncated abstract] The hydraulic properties of the roots of three crop species important to Western Australia were examined: wheat (Triticum aestivum), narrow-leafed lupin (Lupinus angustifolius) and yellow lupin (L. luteus). Generally, the hydraulic conductivity (Lpr) of root systems differs between species and can change in response to adverse conditions. To determine the significance of root anatomy and aquaporin activity on the pathway of water flow through roots, water flow was measured across cell membranes, individual roots and whole root systems. The combination of measurements identified that wheat and lupin roots have contrasting hydraulic properties. Wheat roots absorb water preferentially in the apical region, whereas lupin roots appear to absorb water more evenly along the entire root length. Lupin roots have a greater axial hydraulic conductance than wheat, due to more abundant xylem vessels and axial conductance increases with root length, in conjunction with xylem vessel development. However, water flow through the radial pathway is the limiting factor in whole root hydraulic conductance, in all species. Modelling and the inhibition of aquaporin activity with mercuric chloride demonstrated that radial water flow in wheat roots occurs by a combination of the cell-to-cell and apoplastic pathways, but in lupins, water flow appears to be predominantly apoplastic. Despite the presence of aquaporins in root cell membranes of all species, their role in regulating bulk water flow across roots is not clear in lupins, because of the significance of the apoplastic pathway ... After draining the chambers, the root systems of yellow lupin resumed growth, but there was no subsequent recovery in narrow-leafed lupin root systems. The growth and survival strategies of wheat and lupin root systems are disparate. Wheat root systems are comprised of numerous fine, highly branched, individual roots that extract water near the root tips and have the ability to regulate flow. These attributes may be advantageous in non-uniform or variable environments. Moreover, the ability of wheat roots to regulate flow may not only support survival during waterlogging, but also enhance recovery. In comparison, lupin root systems are designed like conduits, for the rapid uptake and transport of water when conditions are favourable. However, their thick taproots and lack of regulation of water transport or anatomical changes make them unsuitable for very wet soils.
23
Sargent, Eva Lee. "The effects of intraluteal infusion of prostaglandin-synthesis inhibitors on the function of the primate corpus luteum." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184406.
Full textAbstract:
Exogenous prostaglandins (PGs) have been reported to suppress or to promote the function of the primate corpus luteum in vitro and in vivo, but the role of endogenous ovarian prostaglandins in regulating luteal function during the menstrual cycle is unknown. Infusion (via osmotic pump) of the prostaglandin-synthesis inhibitor sodium meclofenamate into the corpus luteum, but not via the jugular vein, during the midluteal phase of the menstrual cycle resulted in a decline in progesterone levels and premature menses in rhesus monkeys (Macaca mulatta). These results suggest that meclofenamate suppresses the production of an obligatory luteotropic prostaglandin or other metabolite of arachidonic acid. We were unable to confirm that ovarian prostaglandin synthesis was diminished during treatment, since we could not consistently measure a gradient in PGE or PGF₂(α) across the ovary. Dispersed cells from the macaque corpus luteum produced PGF₂(α) in vitro. Production was stimulated by exposure to arachidonic acid and was inhibited by meclofenamate and another prostaglandin-synthesis inhibitor, flurbiprofen. Although the two drugs were potent inhibitors of prostaglandin synthesis in vitro, intraluteal infusion of flurbiprofen in monkeys did not mimic the luteolytic effects of meclofenamate. These studies provide the first evidence of an obligatory luteotropic role for a metabolite of arachidonic acid during the primate luteal phase. However the data suggest that the luteolytic effect of meclofenamate in vivo is not mediated entirely by the inhibition of local prostaglandin synthesis. Further studies are needed to determine the mechanism(s) of meclofenamate-induced luteolysis and to identify the putative obligatory luteotropin.
24
Oliveira, Cleber Barbosa de. "Avaliação das células luteais de fêmeas taurinas (Bos taurus taurus) e zebuínas (Bos taurus indicus) /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/105940.
Full textAbstract:
Orientador: César Roberto Esper<br>Banca: José Domingos Guimarães<br>Banca: José Octávio Jacomini<br>Banca: Paulo Henrique Franceschini<br>Banca: Francisco Guilherme Leite<br>Resumo: O objetivo deste trabalho foi determinar o número de células luteais bovinas, comparando fêmeas taurinas com zebuínas no início e final do ciclo estral. Foram coletados corpos lúteos de 16 fêmeas sendo 8 taurinas e 8 zebuínas, distribuídas em 4 grupos sendo coletados os ovários nos dias 3 a 5 (2 grupos: taurino e zebuíno) e 16 a 18 (2 grupos: taurino e zebuíno) do ciclo estral. Os corpos lúteos foram processados para microscopia óptica e avaliouse as células luteais pequenas, grandes e intermediárias, quanto ao número celular, diâmetro, área e perímetro. Os animais taurinos apresentaram maior quantidade de células luteais pequenas que os zebuínos no início do ciclo estral (p<O,05) e final do ciclo estral (p<O,05). Registrou-se diferença nos valores médios do diâmetro, perímetro e área das células luteais grandes, pequenas e intermediárias entre animais taurinos e zebuínos, tanto no início quanto no final do ciclo estral.<br>Abstract: The aim of this work was to determine the number of bovine luteal cells comparing 80S taurus females with 80S indicus females at the beginning and at the end of estrous cycle. Sixteen corpus luteum were collected in eight 80S taurus cattle and in eight 80S indicus cattle, distributed into four groups. The ovaries were collected from the third and the fifth days (two groups: 80S taurus females and 80S indicus females) and from the sixteenth and the eighteenth days (two groups: 80S taurus caUle and 80S indicus cattle) of the estrous cycle. The corpus luteum was processed to optical microscopy and the small, big and intermediate-sized luteal cells were evaluated considering the number of cells, diameter, area and perimeter. The 80S taurus females presented a bigger amount of smallluteal cells than the 80S indicus females at the beginning of the estrous cycle (p,O,05) and at the end of the estrous cycle (p<O,05). The difference in the average values of the diameter, perimeter and the area of big, small and intermediate-sized luteal cells have been registered among 80S taurus cattle and 80S indicus cattle, at the beginning and also at the end of the estrous cycle.<br>Doutor
25
Mlatečková, Tereza. "Antimikrobiální účinky extraktů ze stévie cukrové." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216422.
Full textAbstract:
This master thesis is oriented on study antimicrobial effects extracts and macerates from cure Stevia rebaudiana Bertoni. Teoretical part describes basic information about plant Stevia, summary of health significant matters contained in Stevia and posobilities preparing extracts from Stevia. Antimicrobial effects extracts and macerates from cure Stevia were testing on food-borne bacteria (Bacillus cereus and Micrococcus luteus) and yeasts (Geotrichum candidum and Hansenula anomala). Microorganism, extracts and macerates were chosen on basis previous study (Study of antimicrobial effects Stevia Rebaudiana extracts, Eva Rakovská). For screening antimicrobial activity were determined the growth curves by using turbidimetrie for bacteria and direct treetment metod of cells number for yeasts. Antimicrobial effects were confirmed aplication with diffusion pit method on the agar ranges. From the results flow the testing extracts and macerates from stevia analysed antimicrobial effects. The best effect was demostrated on macerates and the most sensitive was bacteria Micrococcus luteus with the best inhibitoring effects.
26
Ochoa, Julián Camilo [UNESP]. "Expressão gênica do corpo lúteo após pulsos intrauterinos com doses baixas de prostaglandina E1 e F-2 alfa em vacas." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/144324.
Full textAbstract:
Submitted by JULIAN CAMILO OCHOA CUERVO null (julianca-8@hotmail.com) on 2016-10-05T14:14:09Z
No. of bitstreams: 1
PGE1 THESIS. Defensa 09-20-16 VERSION CORREGIDA.pdf: 1584231 bytes, checksum: 86c9e6ac7c8a76a29acc91e5f800c2fd (MD5)<br>Rejected by Ana Paula Grisoto (grisotoana@reitoria.unesp.br), reason: Solicitamos que realize uma nova submissão seguindo a orientação abaixo:
O arquivo submetido está sem a ficha catalográfica.
A versão submetida por você é considerada a versão final da dissertação/tese, portanto não poderá ocorrer qualquer alteração em seu conteúdo após a aprovação.
Corrija esta informação e realize uma nova submissão com o arquivo correto.
Agradecemos a compreensão. on 2016-10-06T17:53:32Z (GMT)<br>Submitted by JULIAN CAMILO OCHOA CUERVO null (julianca-8@hotmail.com) on 2016-10-06T19:41:40Z
No. of bitstreams: 1
PGE1 THESIS. Defensa 09-20-16 VERSION CORREGIDA FICHA CATALOGRAFICA.pdf: 1619163 bytes, checksum: 981fd7797ad79edef078beb911c594c4 (MD5)<br>Rejected by Ana Paula Grisoto (grisotoana@reitoria.unesp.br), reason: Solicitamos que realize uma nova submissão seguindo as orientações abaixo:
Insira a ficha catalográfica antes da folha da comissão examinadora.
Corrija estas informações e realize uma nova submissão com o arquivo correto.
Agradecemos a compreensão. on 2016-10-06T19:53:02Z (GMT)<br>Submitted by JULIAN CAMILO OCHOA CUERVO null (julianca-8@hotmail.com) on 2016-10-06T20:03:19Z
No. of bitstreams: 1
PGE1 THESIS. Defensa 09-20-16 com ficha catalografica.pdf: 1620368 bytes, checksum: d662e09785a2bb73aaa15aaf92df4d19 (MD5)<br>Approved for entry into archive by Ana Paula Grisoto (grisotoana@reitoria.unesp.br) on 2016-10-06T20:12:34Z (GMT) No. of bitstreams: 1
cuervo_jco_me_bot.pdf: 1620368 bytes, checksum: d662e09785a2bb73aaa15aaf92df4d19 (MD5)<br>Made available in DSpace on 2016-10-06T20:12:34Z (GMT). No. of bitstreams: 1
cuervo_jco_me_bot.pdf: 1620368 bytes, checksum: d662e09785a2bb73aaa15aaf92df4d19 (MD5)
Previous issue date: 2016-09-29<br>Em ruminantes a luteólise natural é caraterizada pela liberação de vários pulsos de prostaglandina F2alfa (PGF) produzidos pelo útero. A PGF é o hormônio luteolítico, enquanto a prostaglandina E1 (PGE1) é considerada um mediador luteoprotetor. Em estudos anteriores, infusões com doses baixas de PGF no útero com intervalos de 6 horas (h) resultou em regressão do corpo luteo (CL). A proposta deste experimento é desenvolver um modelo para avaliar o efeito de baixas doses de PGE1, também infundidas no lúmen uterino sobre a resposta luteal à PGF intrauterina (IU). Vacas no dia 10 do ciclo estral receberam infusões IU de salina (0,1 ml de salina + 0,1 ml de DMSO), PGE (2 mg de PGE1 em 0,1ml de DMSO) ou PGF (0,25 mg of PGF em 0,1 ml de salina) em intervalos de 6 h em um desenho experimental 2 X 2. Portanto os animais foram agrupados em quatro tratamentos: SALINA (4 infusões de salina; n=5), PGE (4 infusões de PGE1; n=5), PGF (4 infusões de PGF; n=5) e PGE+PGF (4 infusões de PGE1+PGF; n=5). As concentrações plasmáticas de progesterona (P4) foram dosadas por radioimunoensaio e o volume luteal foi determinado por ultrassonografia transretal. As concentrações circulantes de PGFM e PGEM foram dosadas antes e 10 minutos após as primeiras duas infusões. Biopsias luteais foram coletadas de cada vaca 30 minutos após cada infusão para determiner a expressão de genes em resposta a cada tratamento. As concentrações circulantes de PGFM 10 minutos após as infusões foram maiores em vacas que receberam tratamentos com PGF e PGE+PGF em comparação com as vacas tratadas com salina e PGE. Da mesma forma, as concentrações de PGEM 10 minutos após cada infusão foram maiores em vacas tratadas com PGE e PGE+PGF em comparação com vacas dos grupos salina e PGF. As concentrações de P4 diminuiram no grupo PGF em comparação com o grupo Salina no tempo 12-h (48,9% do controle) após a primeira infusão de PGF, no tempo 24-h (20,2% do controle), e em todos tempos subsequentes (P < 0,05). Não foram encontradas diferenças nas concentrações circulantes de P4 entre os grupos Salina, PGE e PGF+PGE. Houve também uma diminuição do volume luteal entre o grupo PGF e os outros três grupos que foi observada nos tempos 24-h (56,4% do controle), 48-h (30,6% do controle), e 72-h (20,4% do controle) após o tratamento com PGF (P<0.05). Não houve diferenças no volume luteal entre os tratamentos salina, PGE e PGE+PGF. Por tanto, infusões IU simultâneas de baixas doses de PGE1 bloquearam a ação luteolitica de pulsos IU de PGF em vacas, como observado nas mudanças circulantes de P4 e volume luteal. Análises da expressão génica nas biopsias luteais coletadas após o terceiro pulso de PGF, indicam o padrão tipico de expressão de genes em resposta ao tratamento com PGF (FGF2, EGR1, FOS e FAS aumentaram; PTGFR, VEGFA, NR5A1 e STAR diminuiram) e o tratamento PGE+PGF bloqueou completamente as mudanças na expressão destes genes. Infusões IU de PGF e PGE1 parecem ser um excelente modelo para determiner o padrão de expressão de genes envolvidos no efeito luteoprotetor da PGE1.<br>In ruminants, natural luteolysis is characterized by the release of several pulses of prostaglandin F2alpha (PGF) produced by the uterus. Prostaglandin F2alpha is the luteolytic hormone, whereas prostaglandin E1 (PGE1) is considered to be a luteoprotective mediator. In previous studies, low doses of PGF infused into the uterus at 6 hour (h) intervals resulted in regression of the corpus luteum (CL). This study was designed to develop a model to study the effect of low doses of PGE1, also infused into the uterine lumen, on the luteal responses to intrauterine (IU) PGF. Cows on day 10 of the estrous cycle received IU infusions of saline (0,1 ml of saline + 0,1 ml of DMSO), PGE (2 mg of PGE1 in 0,1ml of DMSO) or PGF (0,25 mg of PGF in 0,1 ml of saline) at 6-h intervals in a 2 X 2 experimental design. Thus, there were four treatment groups: SALINE (4 saline infusions; n=5), PGE (4 PGE1 infusions; n=5), PGF (4 PGF infusions; n=5), and PGE+PGF (4 PGE1+PGF infusions; n=5). Radioimmunoassay was used to measure circulating progesterone (P4) concentrations and luteal volume was determined by transrectal ultrasonography. Circulating concentrations of PGFM and PGEM were measured before and 10 minutes after the first two infusions. A luteal biopsy was collected from each cow at 30 minutes after each infusion for later determination of gene expression in response to each treatment. Circulating concentrations of PGFM 10 minutes after infusions were greater in cows receiving treatments with PGF and PGE+PGF than in Saline or PGE-treated cows. In the same way, concentrations of PGEM 10 minutes after infusions, were greater in cows that were treated with PGE and PGE+PGF than in saline and PGF-treated cows. Concentrations of P4 in the PGF group decreased compared to those in the saline group by 12-h (48.9% of control) after first infusion of PGF, at 24-h (20,2% of control), and all subsequent time points (P < 0,05). No differences in circulating P4 concentrations were found between Saline, PGE, and PGF+PGE. There was also a decrease of luteal volume between the PGF group and the other three groups that was detectable at 24 (56,4% of control), 48 (30,6% of control), and 72 (20,4% of control) h after PGF treatment (P<0.05). There were no differences in luteal volume between Saline, PGE, or PGE+PGF. Thus, simultaneous IU infusion of a low dose of PGE1 blocked the luteolytic actions of IU PGF pulses in cattle, as measured by changes in circulating P4 and luteal volume. Analyses of gene expression in the luteal biopsy taken after the third PGF pulse indicate a typical pattern of gene expression in response to the PGF treatments (FGF2, EGR1, FOS and FAS increased; PTGFR, VEGFA, NR5A1 and STAR decreased) and that simultaneous PGE1 treatment completely blocked these gene expression changes. Thus, IU infusion of PGF and PGE1 seems to provide an excellent model for determining the patterns of gene expression involved in the luteoprotective effect of PGE1.
27
Chirinos, Ruiz Flor Andrea, and María Galán Edika Guissela Santa. "Propuesta para la producción y comercialización de harina de hongo comestible Suillus Luteus de la comunidad San Isidro labrador de Marayhuaca para el mercado local en la ciudad de Chiclayo, Perú 2015." Bachelor's thesis, Universidad Católica Santo Toribio de Mogrovejo, 2015. http://tesis.usat.edu.pe/handle/usat/654.
Full textAbstract:
La presente tesis describe una propuesta de plan de negocios para determinar la viabilidad en la producción y comercialización de harina de hongos comestibles, como producto innovador dirigido a la industria de panificación. Siendo el mercado objetivo las principales panaderías de la ciudad. Estudios aplicados al consumidor de pan, revelaron que el 50% de los chiclayanos piensa que el pan no aporta valor nutricional a su dieta, sin embargo consumen entre 4 a 6 panes diarios, sus compras oscilan entre S/. 1.50 a S/. 3.00 y paga por cada unidad entre S/. 0.10 a S/. 0.20. Se halló también un 17% de consumidores que prefieren productos elaborados con ingredientes naturales, estos resultados reflejan las necesidades y atributos valorados por el consumidor chiclayano. La idea de negocio se centra en dos aspectos importantes: primero en los cambios de hábito de consumo saludable los que están transformando el mercado con las nuevas demandas y exigencias. El segundo identifica a los gastos familiares centrados en la alimentación. Frente a estos dos aspectos los productos de panadería son alimentos básicos y forman parte de nuestra dieta tradicional y cultura gastronómica. Un peruano consume en promedio 32 kilos de pan al año, que equivale 2 kilos 700 gr al mes y a 90 gr diario. Considerando estos hechos, el sector panadero se muestra atractivo para la comercialización de harina de hongo comestible (HHC), la misma que tiene como objetivo el desarrollo social, económico y sustentable de las comunidades Altoandinas de la Región de Lambayeque a través de sus productos elaborados bajo estándares de calidad definidos para cumplir con los requerimientos del empresario panadero.<br>Tesis
28
Wegner, Julie Anne. "The interaction of cytosolic-free calcium in PGF(2alpha)-induced luteal regression in ovine corpus luteum." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185210.
Full textAbstract:
The corpus luteum is an endocrine gland which forms in the ovary each reproductive cycle, secretes progesterone, and regresses if pregnancy does not occur. An understanding of the factors and mechanisms that determine the function and lifespan of the corpus luteum is fundamental to the understanding of the mechanisms that cause luteal dysfunction. Prostaglandin F₂(α)(PGF₂(α)) is the primary lutelytic agent in ewes and appears to initiate luteal regression by altering cytosolic-free calcium ([Ca²⁺]ᵢ) and stimulating calcium-dependent intracellular pathways. The primary focus of this dissertation was to investigate the roles of PGF₂(α) and calcium in the regulation of progesterone secretion in the ovine corpus luteum. In fura-2 loaded large cells, PGF₂(α) (0.5 μM) induced a rapid transient increase in [Ca²⁺]ᵢ followed by a sustained elevation of [Ca²⁺]ᵢ. The transient nature of the [Ca²⁺]ᵢ increase was due, at least in part, to the ability of PGF₂(α) to stimulate (p < 0.05)⁴⁵Ca²⁺ efflux. PGF₂(α) did not alter [Ca²⁺]ᵢ in small cells. The PGF₂(α)-induced calcium transient was modified by incubation of large cells in conditions known to alter calcium homeostasis. The transient was attenuated by incubation of large cells in Ca²⁺-free medium (±EGTA). These results suggest that PGF₂(α) induces release of calcium from intracellular calcium pools. However, pre-incubation (2 min) of large cells with 1mM LaCl₃ eliminated the PGF₂(α)-induced calcium transient, suggesting a role of extracellular calcium. Two different results were observed in this study regarding the role of calcium in the regulation of progesterone secretion. First, the inhibitory effect of PGF₂(α) on secretion of progesterone was reduced under conditions that reduced the magnitude of the PGF₂(α)-induced calcium transient. Second, a sustained elevation or reduction in [Ca²⁺]ᵢ level also reduced basal progesterone secretion in large cells. Thus, both phases of the PGF₂(α)-induced [Ca²⁺]ᵢ response, transient increase and sustained elevation, appear to be linked to the inhibitory action of PGF₂(α) on progesterone secretion. Finally, this study provides evidence to suggest that large and small cells differ in their ability to regulate calcium homeostasis.
29
Egawa, Miho. "Ephrin B1 is expressed on human luteinizing granulosa cells in corpora lutea of the early luteal phase : the possible involvement of the B-class Eph-ephrin system during corpus luteum formation." Kyoto University, 2005. http://hdl.handle.net/2433/144492.
Full text
30
Jost, Britta. "Untersuchungen und Kalkulationstabellen zur Schätzung der N₂-Fixierleistung und der N-Flächenbilanz beim Anbau von Lupinus albus und Lupinus luteus in Reinsaat und von Vicia faba und Pisum sativum in Reinsaat und im Gemenge mit Avena sativa." [S.l.] : [s.n.], 2003. http://webdoc.sub.gwdg.de/diss/2003/jost/jost.pdf.
Full text
31
Salfen, Brent Edward. "Effect of the dominant ovarian follicle on the establishment and regulation of postpartum estrous cycles in dairy and beef animals /." free to MU campus, to others for purchase, 2000. http://wwwlib.umi.com/cr/mo/fullcit?p9974683.
Full text
32
Löfkvist, John. "Modifying soil structure using plant roots /." Uppsala : Dept. of Soil Sciences, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/200560.pdf.
Full text
33
Moritz, Stacey. "PGF₂[alpha] inhibits VEGF expression in the corpus luteum in the mid but not early luteal phase of the sheep estrous cycle." Connect to resource, 2006. http://hdl.handle.net/1811/6615.
Full textAbstract:
Thesis (Honors)--Ohio State University, 2006.<br>Title from first page of PDF file. Document formatted into pages: contains 29 p.; also includes graphics. Non-Latin script record Includes bibliographical references (p. 25-29). Available online via Ohio State University's Knowledge Bank.
34
Vacková, Hana. "Studium biologických účinků technického konopí a jeho frakcí." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2017. http://www.nusl.cz/ntk/nusl-316189.
Full textAbstract:
Cannabis is the only plant which contains cannabinoids and thanks to these compounds it has enormous potential. This thesis deals with the analysis of technical hemp. Effects of cannabinoids and methods used for cannabis analysis are discussed in the theoretical part. The experimental part includes spectrophotometric characterization of cannabis, it´s antimicrobial effects and thin layer chromatography analysis. Three sorts of Cannabis sativa L. were analyzed, namely Finola, Fedora and Kompolti. Firstly, the content of polyphenols, flavonoids and antioxidant activity in prepared tinctures were determined. Moreover, antimicrobial test were performed using disk test and turbidity determination. Gram-positive and Gram-negative bacteria and yeast organism were tested. It was found that cannabis tinctures possess good antimicrobial effects. Some of them are comparable with synthetic antibiotics. Finally, thin layer chromatography enabled visualization of cannabinoids in prepared tinctures.
35
Poole, Jacob Marion. "Ovarian Modifications in Mice Exposed to Whole-Body Irradiation." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/honors/99.
Full textAbstract:
This experiment was designed to determine the involvement of varying levels of whole-body irradiation on ovarian follicular and corpora luteal development in mice. Previous research has indicated reduced counts of ovarian follicles and corpora lutea in mice flown in space. These differences may be the result of microgravity, increased exposure to radiation, or some combination of both. Fifty-six mice were divided into three groups (apocynin-treated, nox2 knockout, and wild-type control) before exposure to 0 Gy, 0.5 Gy, or 2.0 Gy radiation. The tissues were harvested, preserved, run through the appropriate paraffin embedding procedures, serially sectioned, mounted on microscope slides, and stained using a standard H&E staining technique. Total and mean follicular and corpora luteal counts were accessed and compared across treatment groups. Mean ovarian weight, mean total reproductive weight, mean ovarian weight percentage of total body weight, mean total reproductive weight percentage of total body weight, and the apparent estrous phase of the animals were also compared. Radiation from 0.5-2.0 Gy had no significant effect on mean ovarian weight, mean total reproductive weight, mean ovarian weight percentage of total body weight, or mean total reproductive weight percentage of total body weight. Radiation from 0.5-2.0 Gy significantly increased mean early-stage follicular count in the wildtype group only. Radiation of 2.0 Gy increased late-stage follicular count across all groups after accounting for mean ovarian percent of total body weight. Radiation of 2.0 Gy significantly increased mean corpora lutea count in the wildtype group only. This result not only suggests that low-dose radiation accelerates oocyte development in the murine ovary, but also that the inaction of NADPH-oxidase (via apocynin inhibition or genetic knockout) may ameliorate some of these effects.
36
Stirling, David. "Functional regulation of the corpus luteum." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/19321.
Full text
37
Smith, George W. "Local regulators of corpus luteum function /." free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9717154.
Full text
38
Ericsson, Daniel, and Ida Lagergren. "Lutens kretslopp på Södra Cell Värö." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-12795.
Full textAbstract:
Rapporten handlar om lutens kretslopp på Södra Cell Värö, dess betydelse för pappersmassaproduktionen samt riskerna som finns vid användning av lut. Lut används när pappersmassa framställs på Södra Cell Värö (sulfatprocess). Kokeriet kokar träflis, tunnlut (svartlut) och vitlut. Luten tillsätts för att lösa upp ligninet i träfibrerna. Lignin är som ett lim som håller ihop träfibrerna. Lutvätskan som lämnar kokeriet kallas för tunnlut och indunstningen torkar tunnluten i flera steg. När luten har torkats kallas den för brännlut (tjocklut) och förbränns i sodapannan. Smältan från sodapannan blandas med svaglut och bildar grönlut. I grönluten tillsätts bränd kalk och bildar kalkmjölk. Ur kalkmjölken separeras vitlut och mesa. Vitluten återinförs till kokeriet och kretsloppet fortsätter. Luten har stor betydelse för produktionen av pappersmassa på Södra Cell Värö. Om någon del i lutkretsloppet inte fungerar tvingas hela fabriken att stanna. Användning av lut är inte riskfritt. Lut är ett frätande ämne och det räcker med små kvantiteter för att orsaka stor skada. Därför är det viktigt att rätt skyddsutrustning används och respekt visas. Om Södra Cell Värö inte skulle använda lut skulle tillverkningsprocessen se annorlunda ut. Mekanisk process eller sulfitprocess skulle användas istället. Fördelarna med att använda lut är att kvalitén på pappersmassan blir bättre. Därför får pappersmassan ett större användningsområde. Nackdelarna med att använda lut är dels risken för olyckor, dels att vedutbytet endast är 50 %. Syftet med rapporten är att ge läsaren lärdom om hur lutens kretslopp fungerar och lutens betydelse på ett massabruk som Södra Cell Värö samt informera om vilka risker det finns vid arbete med lut.
39
Gåfvels, Mats. "Blood flow and metabolism in the corpus luteum of the rat : in vivo and in vitro studies on the ovarian luteal and follicular compartment of the rat." Doctoral thesis, Umeå universitet, Fysiologi, 1987. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-102824.
Full textAbstract:
The ovary undergoes cyclic changes in follicular growth and luteogenesis due to the action of gonadotropins and steroids. The ovary and especially the corpus luteum has an exteremely high blood flow. There is a gap in our knowledge about the physiological role of the high blood flow of the corpus luteum. The production of lactate, progesterone and cyclic AMP of follicles and corpora lutea incubated in vitro was analyzed and related to the tissue content of ATP to elucidate possible connections between oxygen and substrate levels and energy consumption, steroid output and LH responsiveness in vitro. It was also considered of interest to investigate if the oxygen tensions needed for ATP and progesterone production of the follicle and the corpus luteum differed. A corpus luteum model using adult pseudopregnant rats was developed and characterized according to criteria for identification of corpora lutea as well as levels of plasma steroids and gonadotropins. In vitro progesterone production was compared to plasma progesterone levels. The absolute blood flow of corpora lutea of different ages and the response to injection of hCG, noradrenaline and antidiuretic hormone was investigated with the microsphere technique. Relative blood flow changes of follicles and corpora lutea during follicular growth and luteogenesis in vivo were studied by injecting radiolabelled microspheres to anaesthetized immature rats at different time periods after injection of an ovulatory dose of pregnant mare serum gonadotropin. This approach was chosen to investigate the possible relation between follicular/luteal blood flow, steroid output and morphology in relation to the endogenous gonadotropin surge, ovulation and luteogenesis. Hormonal stimulation by injection of hCG and noradrenaline increased total ovarian blood flow but no evidence was found for a parallelism between luteotropism and blood flow. The increasing effect of hCG on ovarian blood flow was partly due to a shunting of blood from the uterus towards the ovary. The antidiuretic hormone potently decreased ovarian and uterine blood flow by 80-90% while blood flow of some other organs (e.g. kidney and spleen) were hardly affected. The corpus luteum of pseudopregnancy was found to produce 15“ 20 times more progesterone in vitro as compared to the preovulatory follicle. The steroidogenesis and energy production of corpora lutea was found to be more sensitive to decreases in oxygen tension in terms of tissue ATP levels and LH responsiveness of progesterone production while the follicle could compensate by increasing glycolysis. A parallelism between follicular/luteal blood flow and progesterone production in vivo was found. It was shown that the formation, growth and progesterone production of the corpus luteum was accompanied by an increase in blood flow as well as vascularization as seen under the light microscope. The endogenous gonadotropin surge did not change follicular blood flow due to the development of a follicular oedema. We hypothesize that the corpus luteum function in vivo and in vitro is dependent on higher energy levels than the preovulatory follicle and that the transformation of the follicle to a corpus luteum is supported by a high nutritive blood flow possibly to support a high demand for energy-rich substrates.<br><p>Diss. (sammanfattning) Umeå : Umeå universitet, 1987, härtill 7 uppsatser.</p><br>digitalisering@umu
40
Nicklin, Leah Theresa. "Functional development of the bovine corpus luteum." Thesis, University of Nottingham, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416289.
Full text
41
Shelton, K. "Endocrine studies on the bovine corpus luteum." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376416.
Full text
42
Rae, Michael T. "Progesterone binding in the bovine corpus luteum." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/21476.
Full textAbstract:
This project was designed to examine the intracellular location of progesterone in bovine luteal cells. These experiments demonstrated the existence of a particulate membrane fraction of luteal cells where much of the endogenous progesterone was located. Results suggest an association between this fraction and the plasma membrane. Moreover, it was shown that these membranes were able to bind exogenous radiolabelled progesterone in a highly specific manner. Other steroids, precursors etc. were bound poorly. Thus, the experiments herein describe the characterisation of this novel progesterone binding site, its distribution in the cells of the bovine corpus luteum and preovulatory follicle, and attempts to purify and identify the progesterone binding protein. Results from these experiments indicated that the progesterone binding site investigated was distinct from classical genomic progesterone receptors. This non-classical progesterone binding protein (NCP<SUB>4</SUB>-BP) was found in both large and small luteal cells of the corpus luteum, though levels were greater in large cells. NCP<SUB>4</SUB>-BP was also found in the theca and granulosa cells of the preovulatory follicle. Binding characteristics of the NCP<SUB>4</SUB>-BP were determined, and partial purification achieved. Results demonstrate that progesterone binding was not due to (i) steroid metabolizing enzymes (ii) non-specific intercalation of steroid into bi-layer membranes or (iii) the genomic progesterone receptor. Studies suggest that the binding site studied may represent a membrane located progesterone receptor with a potential role in the regulation of luteal function in cows.
43
Rodger, Faye Elizabeth. "Cellular interactions in the human corpus luteum." Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/22597.
Full textAbstract:
The corpus luteum is a transient endocrine gland which is essential for the maintenance of early pregnancy in mammals, however the physiological mechanisms which control luteal lifespan are poorly understood. The aim of this thesis is to investigate potential control points in luteal maintenance and regression by studies utilising human tissues from throughout the luteal phase and in simulated early pregnancy. In particular, the roles of luteal growth and angiogenesis, apoptosis and immune cells are examined. Results of these studies indicate that although angiogenesis is maximal in the early luteal phase, growth of blood vessels does not vary during luteal maintenance and regression. Similarly, although the apoptotic protoncogenes bcl-2 and bax are present in the human corpus luteum, expression of these factors remains constant as luteal function changes. Leukocytes are present throughout the luteal lifespan and local cytokine production may be important in causing the ingress of immune cells which is observed during luteal regression. Cells of steroidogenic, vascular and immune cell compartments of the human corpus luteum may interact in complex ways to bring about changes in the function and structure throughout the lifespan of the gland.
44
Sayce, Lynda. "The development of Italianate continuo lutes." Thesis, Open University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368804.
Full text
45
Martinez, Nuñez Gustavo Adolfo. "Densidades histológica e ecográfica do corpo lúteo de éguas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/102611.
Full textAbstract:
A morfologia e a fisiologia ovariana nos equinos têm sido motivo de estudo devido a seu córtex e medula invertidos em comparação às das outras espécies mamíferas e seu ciclo reprodutivo fotoperíodo-dependente e à existência de uma fossa de ovulação. Estes aspectos levam ao profissional do campo da reprodução equina a se interessar em aprofundar cada vez mais no desenvolvimento de pesquisas que possam auxiliar oferecendo métodos e técnicas que ajudem no trabalho de campo. Uma das melhores técnicas utilizadas para o estudo da atividade ovariana têm sido as imagens ultrassonográficas nos seus diferentes modos de operação. Uma das estruturas ovarianas que se pode avaliar e acompanhar com o ultrassom é o Corpo Lúteo (CL), sendo ele vital pela produção de Progesterona (P4), hormônio encarregado da preparação do endométrio para oferecer um ambiente ao embrião. É relatada uma relação positiva entre quantidade de células lúteas e produção de P4. O objetivo deste estudo foi testar uma metodologia que permitisse avaliar a densidade celular por meio da histologia e determinar se existe relação com o numero de pixéis lúteos numa imagem de ultrassom e assim ter um dado confiável para tomar decisões. Foram coletados 29 ovários de éguas crioulas em diferentes fases do diestro, analisados todos dentro de um único grupo. As amostras foram passadas pelo ultrassom para obter a imagem ultrasonográfica salva diretamente do ultrassom. Posteriormente, os CLs foram dessecados para fazer laminas corada pela Hematoxilina Eosina. Os resultados mostraram que entre as variáveis existe correlação inversa de R=-0.61. O coeficiente de determinação achado foi de aproximadamente R2=40% com uma probabilidade P=0.04%. Os resultados indicam que, existem variações no numero de pixéis lúteos que podem explicar em grande percentagem os valores totais do numero de células presentes no tecido lúteo.<br>The ovarian morphology and physiology in horses has been the subject of study due to its reversed cortex and medulla in comparison to other mammalian species, the existance of an ovulation fossa and for its photoperiod-dependent reproductive cycle. These aspects lead to the field practitioner specialist on equine to develop research that may help at fieldwork. One of the best techniques for the study of ovarian activity has been the ultrassonographic images in its different modes of operation. The corpus luteum (CL) is one of the ovarian structures that can be assessed and followed by ultrasound, being vital for the production of progesterone (P4), hormone which prepares the endometrium to provide a good environment for embryo development. A positive relationship between amount of luteal cells and P4 production has been reported before. The aim of this study was to test a methodology that would assess cell density by histology and determine whether there is a correlation between the number of ultrasound image pixels and the luteal cells concentration and thus have a really trusted ultrasound data which might help veterinarians to make decisions. Twenty nine ovaries from criollo mares were collected at different stages of diestrus, and all analyzed within a unique group. Samples were assessed by ultrasound to obtain the ultrasonographic image. After that, the CLs were dried to make microscopy slides stained with hematoxylin-eosin. The results showed that an inverse correlation (r = -0.61) exists between the variables studied. The determination coefficient found was approximately R2 = 40% with a probability of P = 0.04%. The results indicate that there are variations in the number of pixels that can explain differences in the numbers of luteal cells in the corpus luteum tissue.
46
Mennecke, Ute. "Luthers Trostbriefe /." Gütersloh : Güterloher Verlagshaus G. Mohn, 1989. http://catalogue.bnf.fr/ark:/12148/cb36663684q.
Full textAbstract:
Texte remanié de: Diss.--Fachbereich Historisch-Philologische Wissenschaften--Göttingen--Georg-August-Universität, 1986/87. Titre de soutenance : De tristitiae spiritu. Luthers Trostbriefe in der Tradition spätmittelalterlicher Consolatio.
47
Brockhan-Lüdemann, Maren. "Farbdopplersonographische Untersuchungen zur Funktion des bovinen Corpus luteum." Hannover Bibliothek der Tierärztlichen Hochschule Hannover, 2010. http://d-nb.info/1000021076/34.
Full text
48
Amelkina, Olga. "Corpus luteum of the domestic cat and lynx." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2016. http://dx.doi.org/10.18452/17451.
Full textAbstract:
Der Gelbkörper (corpus luteum, CL) ist eine transitorische Drüse, die im Ovar nach der Ovulation gebildet wird und durch ihre Progesteron-(P4)-Produktion die Trächtigkeit unterstützt. Bei allen bisher untersuchten Säugetieren endet die sekretorische Aktivität der CL mit dem Ende der Trächtigkeit oder Laktation, danach werden die CL abgebaut. Der Zyklus von Katzenartigen, wie etwa der Hauskatze, folgt dem gleichen Muster. Anders verläuft der Zyklus bei Luchsen. Beim Eurasischen Luchs (Lynx lynx) und beim Pardelluchs (Lynx pardinus) persistieren die CL nach der Geburt für mehr als zwei Jahre und sezernieren weiter P4. Die vorgestellte Arbeit sollte die Funktion persistierender (per) CL im Luchs untersuchen und die Fortpflanzung von Katzen weiter aufklären. Dazu wurden grundlegende histologische und hormonelle Aspekte der Lutealphase analysiert und der Einfluss des apoptotischen Systems sowie die Rezeptivität gegenüber Steroiden bei der Regulation der CL-Funktion betrachtet. Die CL von Hauskatzen und Luchsen wurden histomorphologisch unterteilt. In allen Proben wurden intraluteales P4 und Östrogene bestimmt. Weiterhin wurde die mRNA- und, wenn möglich, die Proteinexpression der proapoptotischen Faktoren BAX, Caspase-3, FAS, Tumor necrosis factor (TNF), TNF Rezeptor 1 (TNFRSFA1) und der Überlebensfaktoren (BCL2, TNFRSFB1), sowie des Progesteronrezeptors (PGR), der PGR-Membrankomponente (PGRMC) 1 und 2, des Östrogenrezeptors (ESR) 1 und 2, des G-Protein-gekoppelten Östrogenrezeptors 1 (GPER1) und des Androgenrezeptors (AR) gemessen. Die Ergebnisse weisen darauf hin, dass die Lutealphase der Hauskatze durch FAS, Caspase-3 und die TNF Rezeptoren 1 und 2 reguliert sein könnte. Steroide könnten über ihre Rezeptoren PGR, PGRMC1 und PGRMC2, ESR1 und AR wirken. Die physiologische Persistenz der CL beim Luchs könnte über BCL2, FAS, TNFRSFB1, PGRMC1, PGRMC2, ESR1, GPER1 und AR vermittelt werden.<br>Corpus luteum (CL) is a transitory gland which forms in the ovary after ovulation and supports the pregnancy with its production of progesterone (P4). In all mammals studied so far, the CL loses its secretory activity after pregnancy and regresses from the ovary. The feline luteal cycle follows the same pattern, and CL of the domestic cat functionally and structurally regress after lactation. However, the story is different for the lynx. In the Eurasian (Lynx lynx) and Iberian (Lynx pardinus) lynx, CL persist after parturition, weaning and for up to two years, still retaining their ability to secrete P4. Current work was initiated to understand the control of unusual persistent (per) CL in lynx and to learn more about feline reproduction in general. For this, studies on the basic histological and endocrinological aspects of the feline luteal phase, as well as potential involvement of systems of apoptosis and steroid receptivity in the CL regulation were performed. Collected CL from domestic cats and lynx were classified based on their histomorphology. In all samples, intraluteal P4 and estrogens were measured. Moreover, mRNA and where possible protein levels were determined for pro-apoptotic BAX, caspase-3, FAS, tumor necrosis factor (TNF), TNF receptor 1 (TNFRSFA1), pro-survival BCL2, TNFRSFB1, and for progesterone receptor (PGR), PGR membrane components (PGRMC) 1 and 2, estrogen receptors (ESR) 1 and 2, G protein-coupled estrogen receptor 1 (GPER1) and androgen receptor (AR). The results suggest that the luteal phase of the domestic cat is potentially regulated by caspase-3, FAS, TNFRSF1A, TNFRSF1B, and by actions of steroids via PGR, PGRMC1, PGRMC2, ESR1 and AR. Physiological persistence of Iberian lynx CL might be mediated by BCL2, FAS, TNFRSFB1, PGRMC1, PGRMC2, ESR1, GPER1 and AR. Current work indicates profound differences between the CL function and regulation in domestic cats and lynx, and promotes a highly species-specific approach in reproduction studies.
49
Choudhary, Ekta. "Prostaglandin F₂[subscript alpha] (PGF₂[subscript alpha])--independent and--dependent regulation of the bovine luteal endothelin system." Morgantown, W. Va. : [West Virginia University Libraries], 2005. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4182.
Full textAbstract:
Thesis (M.S.)--West Virginia University, 2005.<br>Title from document title page. Document formatted into pages; contains viii, 40 p. : ill. Includes abstract. Includes bibliographical references (p. 36-40).
50
ALMEIDA, José Bruno Araújo de. "Efeito de características ambientais no uso dos canais-de-maré por três espécies de peixes bentófagos durante o período transição-seca no estuário de Curuçá (Pará-Brasil)." Universidade Federal do Pará, 2009. http://repositorio.ufpa.br/jspui/handle/2011/3504.
Full textAbstract:
Submitted by Edisangela Bastos (edisangela@ufpa.br) on 2013-02-26T21:41:27Z
No. of bitstreams: 2
license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Dissertacao_EfeitoCaracteristicasAmbientais.pdf: 1424534 bytes, checksum: 90b0a9ccb4539c1936ab56dfa68d26b4 (MD5)<br>Approved for entry into archive by Ana Rosa Silva(arosa@ufpa.br) on 2013-03-01T12:35:57Z (GMT) No. of bitstreams: 2
license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Dissertacao_EfeitoCaracteristicasAmbientais.pdf: 1424534 bytes, checksum: 90b0a9ccb4539c1936ab56dfa68d26b4 (MD5)<br>Made available in DSpace on 2013-03-01T12:35:57Z (GMT). No. of bitstreams: 2
license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)
Dissertacao_EfeitoCaracteristicasAmbientais.pdf: 1424534 bytes, checksum: 90b0a9ccb4539c1936ab56dfa68d26b4 (MD5)
Previous issue date: 2009<br>Fundação Gordon e Betty Moore<br>O estuário de Curuçá é parte de uma Unidade de Conservação (RESEX Mãe Grande de Curuçá) desde 2002 e tem como principais cursos d’agua o Rio Curuçá e do Furo Muriá. O presente estudo objetivou analisar o efeito de características ambientais no uso dos canais-de-maré pelas espécies bentófagas Colomesus psittacus, Sciades herzbergii e Genyatremus luteus. A análise envolveu a relação de dados ambientais (salinidade, área de inundação e cobertura arbórea) com dados bióticos das três espécies estudadas (densidade, biomassa, intensidade alimentar e distribuição dos comprimentos totais). A primeira expedição ocorreu em julho de 2008 a fim de coletar a ictiofauna e a segunda em outubro de 2008 para realizar o inventário arbóreo, sendo que cada campanha teve a duração de três dias. Por todo o estuário de Curuçá, mais precisamente, ao longo do Rio Curuçá e do Furo Muriá, foram distribuídos um total de seis sítios de coleta, onde no interior de cada sítio foram amostrados dois canais-de-maré, perfazendo um total de doze canais-de-maré amostrados. As espécies C. psittacus, S. herzbergii e G. luteus estiveram entre as cinco mais abundantes e ocorreram em todos os sítios, com exceção do S. herzbergii, o qual foi capturado somente em cinco dos seis sítios. As três espécies estudadas apresentaram, entre os sítios, diferenças altamente significativas para as variações de suas médias de densidade, biomassa, índice de plenitude e comprimento total, com exceção de G. luteus que não apresentou diferenças estatísticas em suas médias de comprimento total. Todas as variações das médias das características ambientais referentes à cobertura arbórea (densidade, altura e diâmetro), assim como, a área de inundação dos canais-de-maré apresentaram significativas diferenças entre os sítios. Neste trabalho foi possível verificar: a influência do gradiente de salinidade na distribuição das espécies estudadas no interior do estuário; as relações espaciais entre a área de inundação e disponibilidade de recurso alimentar, onde os canais com maior área inundada mostraram-se mais favoráveis ao forrageio da ictiofauna bentófaga; que provavelmente, existe um melhor rendimento alimentar quando as espécies bentófagas frequentam os canais-de-maré cercados por vegetação desenvolvida.<br>The Curuçá estuary is part of a Conservation Unit (RESEX Great Mother of Curuçá) since 2002 and has as main water courses the Curuçá River and Muriá bore. This study aimed to analyze the effect of environmental characteristics in the use of the creek on the benthophagous species Colomesus psittacus, Sciades herzbergii and Genyatremus luteus. The analysis involved the relationship of environmental data (salinity, submergence area and tree cover) and biotic data from the three species studied (density, biomass, feeding intensity and distribution of total length). The first expedition took place in July 2008 to collect the fish fauna and the second in October 2008 to conduct the tree inventory, and each campaign lasted for three days. For all the estuary of Curuçá, more precisely, along the Curuçá River and Muriá bore were allotted a total of six study sites, where within each site were sampled two creeks, making a total of twelve creeks sampled. The species C. psittacus, S. herzbergii and G. luteus were among the five most abundant and occurred in all sites, except for S. herzbergii, which was captured only in five of the six sites. The three species showed, among sites, highly significant differences for changes in their average density, biomass, index of fullness and length, except for G. luteus which did not show statistical differences in their average total length. All variations of the average environmental characteristics related to tree cover (density, height and diameter), as well as, the area of flood creek showed significant differences between sites. This study has showed: the influence of salinity gradient in the distribution of species within the estuary; spatial relations between the flooded area and availability of food resources, where the creek with larger flooded area were more conducive to forage fish fauna benthophagous; likely, there is a better yield when feeding species benthophagous occurs in creek surrounded by vegetation developed.