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Academic literature on the topic 'LuxAB'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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Journal articles on the topic "LuxAB"

1

Katayama, Mitsunori, Nicholas F. Tsinoremas, Takao Kondo, and Susan S. Golden. "cpmA, a Gene Involved in an Output Pathway of the Cyanobacterial Circadian System." Journal of Bacteriology 181, no. 11 (June 1, 1999): 3516–24. http://dx.doi.org/10.1128/jb.181.11.3516-3524.1999.

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ABSTRACT We generated random mutations in Synechococcus sp. strain PCC 7942 to look for genes of output pathways in the cyanobacterial circadian system. A derivative of transposon Tn5 was introduced into the chromosomes of reporter strains in which cyanobacterial promoters drive the Vibrio harveyi luxAB genes and produce an oscillation of bioluminescence as a function of circadian gene expression. Among low-amplitude mutants, one mutant, tnp6, had an insertion in a 780-bp open reading frame. The tnp6 mutation produced an altered circadian phasing phenotype in the expression rhythms of psbAI::luxAB,psbAII::luxAB, andkaiA::luxAB but had no or little effect on those of psbAIII::luxAB,purF::luxAB,kaiB::luxAB,rpoD2::luxAB,ndhD::luxAB, andconII::luxAB. This suggests that the interrupted gene in tnp6, named cpmA (circadian phase modifier), is part of a circadian output pathway that regulates the expression rhythms of psbAI, psbAII, andkaiA.

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SHARP, NATASHA J., JOSHUA P. VANDAMM, IAN J. MOLINEUX, and DAVID A. SCHOFIELD. "Rapid Detection of Bacillus anthracis in Complex Food Matrices Using Phage-Mediated Bioluminescence." Journal of Food Protection 78, no. 5 (May 1, 2015): 963–68. http://dx.doi.org/10.4315/0362-028x.jfp-14-534.

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Bacillus anthracis, the causative agent of anthrax, is considered a high-priority agent that may be used in a food-related terrorist attack because it can be contracted by ingestion and it also forms spores with heat and chemical resistance. Thus, novel surveillance methodologies to detect B. anthracis on adulterated foods are important for bioterrorism preparedness. We describe the development of a phage-based bioluminescence assay for the detection of B. anthracis on deliberately contaminated foods. We previously engineered the B. anthracis phage Wβ with genes encoding bacterial luciferase (luxA and luxB) to create a “light-tagged” reporter (Wβ::luxAB) that is able to rapidly detect B. anthracis by transducing a bioluminescent signal response. Here, we investigate the ability of Wβ::luxAB to detect B. anthracis Sterne, an attenuated select agent strain, in inoculated food (ground beef) and milk (2%, baby formula, and half and half) matrices after incubation with spores for 72 h at 4°C as per AOAC testing guidelines. The majority of B. anthracis bacilli remained in spore form, and thus were potentially infectious, within each of the liquid matrices for 14 days. Detection limits were 80 CFU/ml after 7 h of enrichment; sensitivity of detection increased to 8 CFU/ml when enrichment was extended to 16 h. The limit of detection in ground beef was 3.2 × 103 CFU/g after 7 h of enrichment, improving to 3.2 × 102 CFU/g after 16 h. Because the time to result is rapid and minimal processing is required, and because gastrointestinal anthrax can be fatal, the reporter technology displays promise for the protection of our food supply following a deliberate release of this priority pathogen.

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O'Connell, Kevin P., Ann M. Gustafson, M. Deane Lehmann, and Michael F. Thomashow. "Identification of Cold Shock Gene Loci inSinorhizobium meliloti by Using a luxABReporter Transposon." Applied and Environmental Microbiology 66, no. 1 (January 1, 2000): 401–5. http://dx.doi.org/10.1128/aem.66.1.401-405.2000.

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ABSTRACT Using a luxAB reporter transposon, seven mutants ofSinorhizobium meliloti were identified as containing insertions in four cold shock loci. LuxAB activity was strongly induced (25- to 160-fold) after a temperature shift from 30 to 15°C. The transposon and flanking host DNA from each mutant was cloned, and the nucleic acid sequence of the insertion site was determined. Unexpectedly, five of the seven luxAB mutants contained transposon insertions in the 16S and 23S rRNA genes of two of the threerrn operons of S. meliloti. Directed insertion of luxAB genes into each of the three rrnoperons revealed that all three operons were similarly affected by cold shock. Two other insertions were found to be located downstream of a homolog of the major Escherichia coli cold shock gene,cspA. Although the cold shock loci were highly induced in response to a shift to low temperature, none of the insertions resulted in a statistically significant decrease in growth rate at 15°C.

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Cohen, Michael F., Yasuko Sakihama, Yojiro C. Takagi, Toshio Ichiba, and Hideo Yamasaki. "Synergistic Effect of Deoxyanthocyanins from Symbiotic Fern Azolla spp. on hrmA Gene Induction in the Cyanobacterium Nostoc punctiforme." Molecular Plant-Microbe Interactions® 15, no. 9 (September 2002): 875–82. http://dx.doi.org/10.1094/mpmi.2002.15.9.875.

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The hrmA gene of the N2-fixing cyanobacterium Nostoc punctiforme functions in repressing the formation of transitory motile filaments, termed hormogonia, by plant-associated vegetative filaments. Here, we report that anthocyanins can contribute to induction of hrmA expression. Aqueous extract from fronds of the fern Azolla pinnata, a host of symbiotic Nostoc spp., was found to be a potent inducer of hrmA-luxAB in N. punctiforme strain UCD 328. The hrmA-luxAB inducing activities of A. pinnata, as well as Azolla filiculoides, were positively correlated with levels of frond deoxyanthocyanins. Analyses of the deoxyanthocyanins in frond extracts revealed, in order of predominance, an acetylated glycoside derivative of luteolinidin (m/z 475) and of apigeninidin (m/z 459) and minor amounts of a second luteolinidin derivative. At up to 150 μM, a purified preparation of deoxyanthocyanins only weakly induced hrmA-luxAB on its own, but mixtures with hrmA-luxAB inducers (A. filiculoides extract or the flavonoid naringin) synergistically doubled to tripled their inducing activities. These results suggest that appropriately localized deoxyanthocyanins could function in plant-mediated mechanisms for repressing Nostoc spp. hormogonium formation.

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Gustafson, Ann M., Kevin P. O'Connell, and Michael F. Thomashow. "Regulation ofSinorhizobium meliloti1021rrnA-reporter gene fusions in response to cold shock." Canadian Journal of Microbiology 48, no. 9 (September 1, 2002): 821–30. http://dx.doi.org/10.1139/w02-078.

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We previously reported that mutants of Sinorhizobium meliloti 1021 carrying luxAB insertions in each of the three 16S rRNA genes exhibited a dramatic ([Formula: see text]28-fold) increase in luminescence following a temperature downshift from 30 to 15°C. These results raised the possibility that the rRNA operons (rrn) of S. meliloti were cold shock loci. In testing this possibility, we found that fusion of the S. meliloti 1021 rrnA promoter to two different reporter genes, luxAB and uidA, resulted in hybrid genes that were transiently upregulated (as measured by transcript accumulation) about four- to sixfold in response to a temperature downshift. These results are consistent with the hypothesis that the rrn promoters are transiently upregulated in response to cold shock. However, much of the apparent cold shock regulation of the initial luxAB insertions was due to an unexpected mechanism: an apparent temperature-dependent inhibition of translation. Specifically, the rrnA sequences from +1 to +172 (relative to the start of transcription) were found to greatly decrease the ability of S. meliloti to translate hybrid rrnluxAB transcripts into active protein at 30°C. This effect, however, was largely eliminated at 15°C. Possible mechanisms for the apparent transient increase in rrnA promoter activity and temperature-dependent inhibition of translation are discussed.Key words: rhizobium, gene expression, luciferase.

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Wiles, Siouxsie, Kathryn Ferguson, Martha Stefanidou, Douglas B. Young, and Brian D. Robertson. "Alternative Luciferase for Monitoring Bacterial Cells under Adverse Conditions." Applied and Environmental Microbiology 71, no. 7 (July 2005): 3427–32. http://dx.doi.org/10.1128/aem.71.7.3427-3432.2005.

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ABSTRACT The availability of cloned luciferase genes from fireflies (luc) and from bacteria (luxAB) has led to the widespread use of bioluminescence as a reporter to measure cell viability and gene expression. The most commonly occurring bioluminescence system in nature is the deep-sea imidazolopyrazine bioluminescence system. Coelenterazine is an imidazolopyrazine derivative which, when oxidized by an appropriate luciferase enzyme, produces carbon dioxide, coelenteramide, and light. The luciferase from the marine copepod Gaussia princeps (Gluc) has recently been cloned. We expressed the Gluc gene in Mycobacterium smegmatis using a shuttle vector and compared its performance with that of an existing luxAB reporter. In contrast to luxAB, the Gluc luciferase retained its luminescence output in the stationary phase of growth and exhibited enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature. The work presented here demonstrated the utility of the copepod luciferase bioluminescent reporter as an alternative to bacterial luciferase, particularly for monitoring responses to environmental stress stimuli.

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Escher, A., D. J. O'Kane, J. Lee, and A. A. Szalay. "Bacterial luciferase alpha beta fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature." Proceedings of the National Academy of Sciences 86, no. 17 (September 1989): 6528–32. http://dx.doi.org/10.1073/pnas.86.17.6528.

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A 2.2-kilobase-pair (kbp) DNA fragment from Vibrio harveyi contains the luxA and luxB genes separated by a 26-base-pair (bp) intergenic region. The two genes were converted to a single open reading frame by site-specific mutagenesis. A full-length fusion protein is obtained when the new gene is placed under transcriptional control of a T7 promoter in Escherichia coli. Bioluminescence of colonies containing the gene fusion is 0.002% of the wild-type luciferase [alkanal monooxygenase (FMN-linked); alkanal, reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] at 37 degrees C. Growth at 23 degrees C results in a greater than 50,000-fold increase in light emission in cells containing fusion protein, whereas only a 3-fold increase in observed with cells containing the luxAB dicistron. Purified fusion protein isolated from E. coli grown at 19 degrees C exists in both monomeric and dimeric forms with specific bioluminescence activities comparable to the heterodimeric wild-type enzyme at 23 degrees C and 37 degrees C. These findings show that the alpha beta fusion polypeptide is functional as a monomer and suggest that its folding is drastically affected at elevated temperature. We hypothesize that the two-subunit bacterial luciferase may have evolved from a monomer as a result of a temperature increase in the environment.

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HOSSEINKHANI, Saman, Rose SZITTNER, and Edward A. MEIGHEN. "Random mutagenesis of bacterial luciferase: critical role of Glu175 in the control of luminescence decay." Biochemical Journal 385, no. 2 (January 7, 2005): 575–80. http://dx.doi.org/10.1042/bj20040863.

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Bacterial luciferases (LuxAB) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. Luciferases from Vibrio harveyi and Xenorhabdus (Photorhabdus) luminescens have slow decay rates, and those from the Photobacterium genus, such as Photobacterium fisheri, P. phosphoreum and P. leiognathi, have rapid decay rates. By substitution of a 67-amino-acid stretch of P. phosphoreum LuxA in the central region of the LuxA subunit, the ‘slow’ X. luminescens luciferase was converted into a chimaeric luciferase with a significantly more rapid decay rate [Valkova, Szittner and Meighen (1999) Biochemistry 38, 13820–13828]. To understand better the role of specific residues in the classification of luciferases as slow and fast decay, we have conducted random mutagenesis on this region. One of the mutants generated by a single mutation on LuxA at position 175 [E175G (Glu175→Gly)] resulted in the ‘slow decay’ X. luminescens luciferase being converted into a luciferase with a significantly more rapid decay rate. These results indicate the importance of Glu175 in LuxA as a critical residue for differentiating between ‘slow’ and ‘fast’ luciferases and show that this distinction is primarily due to differences in aldehyde affinity and in the decomposition of the luciferase–flavin–oxygen intermediate.

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Bayer, Thomas, Aileen Becker, Henrik Terholsen, In Jung Kim, Ina Menyes, Saskia Buchwald, Kathleen Balke, Suvi Santala, Steven C. Almo, and Uwe T. Bornscheuer. "LuxAB-Based Microbial Cell Factories for the Sensing, Manufacturing and Transformation of Industrial Aldehydes." Catalysts 11, no. 8 (August 10, 2021): 953. http://dx.doi.org/10.3390/catal11080953.

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The application of genetically encoded biosensors enables the detection of small molecules in living cells and has facilitated the characterization of enzymes, their directed evolution and the engineering of (natural) metabolic pathways. In this work, the LuxAB biosensor system from Photorhabdus luminescens was implemented in Escherichia coli to monitor the enzymatic production of aldehydes from primary alcohols and carboxylic acid substrates. A simple high-throughput assay utilized the bacterial luciferase—previously reported to only accept aliphatic long-chain aldehydes—to detect structurally diverse aldehydes, including aromatic and monoterpene aldehydes. LuxAB was used to screen the substrate scopes of three prokaryotic oxidoreductases: an alcohol dehydrogenase (Pseudomonas putida), a choline oxidase variant (Arthrobacter chlorophenolicus) and a carboxylic acid reductase (Mycobacterium marinum). Consequently, high-value aldehydes such as cinnamaldehyde, citral and citronellal could be produced in vivo in up to 80% yield. Furthermore, the dual role of LuxAB as sensor and monooxygenase, emitting bioluminescence through the oxidation of aldehydes to the corresponding carboxylates, promises implementation in artificial enzyme cascades for the synthesis of carboxylic acids. These findings advance the bio-based detection, preparation and transformation of industrially important aldehydes in living cells.

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Wang, Chunxia, Daoben Wang, and Qi Zhou. "Colonization and persistence of a plant growth-promoting bacterium Pseudomonas fluorescens strain CS85, on roots of cotton seedlings." Canadian Journal of Microbiology 50, no. 7 (July 1, 2004): 475–81. http://dx.doi.org/10.1139/w04-040.

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Pseudomonas fluorescens CS85, which was previously isolated from the rhizosphere of cotton seedlings, acts as both a plant growth-promoting bacterium and a biocontrol agent against cotton pathogens, including Rhizoctonia solani, Colletotrichum gossypii, Fusarium oxysporum f sp. vasinfectum, and Verticillium dahliae. Strain CS85 was labeled separately with luxAB and gusA. The labeled strains were stably maintained and had high levels of expression of the marker genes, luxAB and gusA, after successive transfers on nonselective medium, long-term preservation, and after recovery from soil. The labeled strains displayed similar biocontrol characteristics (e.g., antibiosis, effects of growth -promotion and disease -control) to the original strain. The labeled strains colonized all surfaces of the young plant root zones, such as roots hairs and lateral roots, although the distribution of the labeled strains on the root surfaces was not uniform. Moreover, the population densities of the labeled strains on the root surface were stably maintained at high levels during the first 2 weeks of plant growth in the native soil, so that about 107–108 CFU/g root were detected, then decreased gradually. Nevertheless, approximately 106 CFU/g root of the labeled strains were observed on the root surfaces 35 d after planting.Key words: plant growth-promoting bacteria, luxAB, gusA, root colonization.

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Dissertations / Theses on the topic "LuxAB"

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Boyanapalli, Ramakrishna Bharadwaj. "Construction and Characterization of Cyanobacterial Bioreporters to Assess Nutrient (P, Fe) Availability in Marine Environments." Bowling Green State University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1145826324.

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Fulayfil, Nada Rashid. "Characterization of LuxA of novel strains of the genus Shewanella." FIU Digital Commons, 1994. http://digitalcommons.fiu.edu/etd/3425.

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Bioluminescence is a trait observed among different genera and families of bacteria. In this study part of the luxA gene was characterized from the new MS isolates and compared to luxA of other bacteria. The polymerase chain reaction (PCR) was used to amplify a fragment of the luxA gene of strain MS32 and Vibrio harveyi. These fragments were used as probes in hybridization experiments with luminous and nonluminous bacteria. The results from these experiments suggest that some nonluminous species may possess lux like regions in their chromosomal DNA and that luxA probes can demonstrate species identity. The MS32 luxA fragment was also sequenced and used in a phylogenetic analysis to identify the taxonomic affinities of MS strains. It was found that MS1 and MS32 were closely related, however, Shewanella hanedai was not. Thus there was a concordance between the phenotypic and genotypic approaches, which will help in establishing a consistent taxonomic affinity between these bacteria.

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Di, Bonito Rita. "Use of LuxA sequences for investigation on Luciferases kinetics and characterization of luminous bacteria." FIU Digital Commons, 2002. http://digitalcommons.fiu.edu/etd/2796.

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Known luminous bacteria belong to the genera Vibrio, Photobacterium, Shewanella, and Photorhabdus. The enzyme luciferase catalyzing the luminous reaction is composed by the α and β polypeptides and subunit α is responsible for substrate binding and catalytic activities. Luciferases are classified into "slow " of Vibrio harveyi and "fast" of Photobacterium sps. on the basis of enzyme kinetics. Shewanella woodyi has "intermediate" kinetics. This research has tested the hypothesis of existence of three kinetic classes by sequencing luxA gene (coding for c subunit) of new strains and comparing these clusters to phenotypic analysis and sequencing of 16S rRNA. Phenotypic analysis has shown strains distinct from the known. LuxA amino acids and nucleotides and 16S rRNA sequences have shown 5 major lineages corresponding to known species. A dlade distinct from the known species was present. Geographic location and fish habitat didn't affect the distribution of strains.

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Al-Barkuli, al-Hudayri H. A. Al-R. "An edition of 'Lubb al-Lubab wa Nuzhat al-Ahbab' by Ahmad b. Muhammad b. Ibrahim al-Ash'ari." Thesis, University of Exeter, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355188.

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Valkova, Nelly. "Determination of the luminescence decay rate and of the flavin binding affinity by the luxA carboxyl terminal regions in chimeric bacterial luciferases." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0031/MQ64471.pdf.

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Mohd, Zarif Muhammad Mustaqim. "Jawah hadith scholarship in the nineteenth century : a comparative study of the adaptions of Lubab al-Hadith composed by Nawawi of Banten (d.1314/1897) and Wan Ali of Kelantan (d.1331/1913)." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/3448.

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Hadīth scholarship and its erudition among the Jāwah or the Muslims from the Malay Archipelago (the term applied to them in the Hejaz) in the periods prior to the twentieth century is almost a neglected area of study on Islam and its development in the Southeast Asian region. While this may be surprising considering the sublime status and influence of hadīth on the religious outlook of the Jāwah, perhaps the dearth of surviving materials on hadīth and its study during these periods might have also aggravated this apparent gap in their religious and intellectual history in the pre-modern era. However, this study proposes that despite the feasibility of an early presence of hadīth studies and its scholarship among the Jāwah, it was actually in the nineteenth century that significant development in its scholarship and discourse took place through Lubāb al-Hadīth. This is a collection of four hundred traditions attributed to al-Suyūtī (d. 911/1505), which has managed to attract serious scholarly interests from two important Jāwah scholars in Mecca namely, Nawawī of Banten (d. 1314/1897) and Wan ‘Alī of Kelantan (d. 1331/1913), who undertook their adaptations and commentaries of the text. Even though both scholars shared similar cultural and scholarly milieu of Arabia, their approaches, methods, and choices of languages in commenting on the text are markedly divergent. The fact that both works are still being distributed and read until the present day indicates their significance and relevance as an influential legacy of Jāwah h}adīth scholarship and its discourse in the nineteenth century. Thus, this study examines the important issue of hadīth scholarship in the nineteenth century through the case of Lubāb al-Hadīth and a comparative study of its two commentaries as mentioned above. Although the primary focus of discussion is on their methods on hadīth and selected religious views as presented in their commentaries, the anonymities surrounding the origin, authorship and significance of the base work is also analyzed. In turn, this has lead to a more detailed account on the place and influence of these works on the general development and characteristics of Jāwah hadīth scholarship and its discourse in the nineteenth century which also had their impacts in later years.

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Peat, Scott M. "Utilization of Phylogenetic Systematics, Molecular Evolution, and Comparative Transcriptomics to Address Aspects of Nematode and Bacterial Evolution." BYU ScholarsArchive, 2010. https://scholarsarchive.byu.edu/etd/2535.

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Both insect parasitic/entomopathogenic nematodes and plant parasitic nematodes are of great economic importance. Insect parasitic/entomopathogenic nematodes provide an environmentally safe and effective method to control numerous insect pests worldwide. Alternatively, plant parasitic nematodes cause billions of dollars in crop loss worldwide. Because of these impacts, it is important to understand how these nematodes evolve, and, in the case of entomopathogenic nematodes, how their bacterial symbionts evolve. This dissertation contains six chapters. Chapter one is a review of DNA markers and their use in the phylogenetic systematics of entomopathogenic and insect-parasitic nematodes as well as a review of phylogenetic, co-phylogenetic, and population genetic methodologies. Chapter two characterizes positive destabilizing selection on the luxA gene of bioluminescent bacteria. Our data suggests that bacterial ecology and environmental osmolarity are likely driving the evolution of the luxA gene in bioluminescent bacteria. Chapter 3 examines relationships among bacteria within the genus Photorhabdus. Our analyses produced the most robust phylogenetic hypothesis to date for the genus Photorhabdus. Additionally, we show that glnA is particularly useful in resolving specific and intra-specific relationships poorly resolved in other studies. We conclude that P. asymbiotica is the sister group to P. luminescens and that the new strains HIT and JUN should be given a new group designation within P. asymbiotica. Chapter 4 characterizes the morphology of the head and feeding apparatus of fungal feeding and insect infective female morphs of the nematode Deladenus siricidicola using scanning electron microscopy. Results showed dramatic differences in head, face, and stylet morphology between the two D. siricidicola female morphs that were not detected in previous studies using only light microscopy. Chapter five utilizes comparative transciptomics to identify putative plant and insect parasitism genes in the nematode Deladenus siricidicola. Results from this study provide the first transcriptomic characterization for the nematode Deladenus siricidicola and for an insect parasitic member of the nematode infraorder Tylenchomorpha. Additionally, numerous plant parasitism gene homologues were discovered in both D. siricidicola libraries suggesting that this nematode has co-opted these plant parasitism genes for other functions. Chapter six utilizes a phylogenomic approach to estimate the phylogeny of the nematode infraorder Tylenchomorpha.

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李馥君. "利用luxAB報導基因探討Xanthomonascampestrispv.campestris緊急求救反應之調控機制." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/69551428271842281083.

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碩士
國立中興大學
分子生物學研究所
91
Abstract To elucidate the regulatory mechanism of SOS response in Xanthomonas campestris pv. campestris (Xc17), activities of six DNA damage inducible promoters, recA, 2846-lexA, 5360-lexA, sulA, umuD and algU were analyzed. The promoter regions of these genes were cloned and fused to the luxAB gene from Vibrio fischeri. These promoter fusion luxAB reporter genes were then introduced into Xc17 chromosome by homologous recombination and identified by Southern blot. By measuring the luciferase activity, the transcriptional activities of these promoters in the presence or absence of mitomycin C were determined. Results of luciferase activity assay revealed that all the tested promoters except umuD promoter were DNA—damage inducible. The recA promoter was constitutively expressed during the MMC treatment and has 4 to 7 times higher activity when compared to that in the absence of mitomycin C. However, the 2846-lexA, 5360-lexA and sulA promoters could only be induced after longer exposure to MMC. To investigate the role of Xc17 lexA on the expression of recA, strains of recA-luxAB reporter gene in Xc17 2846- lexA- mutant and with 2846-lexA or 5360-lexA overexpression plasmid were constructed. Results showed that the induction ratio of recA promoter was decreased in 2846-lexA or 5360-lexA overexpression transformants. Although evidences suggested that Xc17 LexA can not specifically bind to recA promoter in vitro, the results indicated that lexA genes have an effect on the expression of recA gene. Moreover, the promoter activities of 2846-lexA and sulA were decreased in Xc17 2846-lexA- strain and the DNA damage induce expression of these promoters were at same level. Furthermore, in order to clone gene from Xc17 that allow expression of SOS response in E. coli, genomic DNA library of Xc17 was constructed on pSK- plasmid. The selection was carried out in E. coli ER1992 that contains the lacZ gene under the control of SOS—inducible dinD promoter. A total of 200 transformants that exhibited blue color on X-gal plate were identified and sequenced. Among these selected clones, four genes (ruvB、endonuclease V、groESL and xveII) were selected for further characterization. These four target genes were amplified and cloned into broad range host vector (pBBad22T) and then introduced into the Xc17 recA-luxAB reporter gene construct (XcRPlux). Results revealed that expression of recA promoter were increased three times when XveII and Endonuclease V were overexpressed. Moreover, by using two-dimensional gel electrophoresis analysis, the different expression patterns of Xc17 proteins in wild type and 2846-lexA- or recA- mutant were identified.

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ZHANG, WU-ZHONG, and 張武總. "以luxA與luxB為報導基因之載體構築與其在菸草細胞中的表現." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/99922432084528752278.

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Fernandes, Daniela Filipa de Brito Pais. "Estudo e conceção de um novo dispositivo médico auxiliar para a remoção de dentes e raízes." Master's thesis, 2012. http://hdl.handle.net/1822/23539.

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Dissertação de mestrado integrado em Engenharia Biomédica
Este trabalho tem como principal objectivo estudar e conceber um novo dispositivo médico que permita auxiliar o médico dentista aquando do processo de extração dentária. Pretende-se desenvolver um dispositivo útil, eficiente e seguro quer para o especialista quer para o paciente. Este surge como uma grande inovação nesta área, pois permitirá ao utilizador realizar quatro etapas diferentes – cortar, segurar, luxar e extrair – utilizando sempre o mesmo dispositivo. Além disso, poderá também ser utilizado para diferentes tipos de dentes, dado que as pontas ativas poderão ser alteradas de acordo com cada caso específico. No entanto, um dos pontos mais fortes assenta no fato do dispositivo possuir um sistema vibratório que auxiliará o médico dentista aquando do processo de luxação dentária, reduzindo então os esforços e as forças necessárias a aplicar pelo mesmo. Da mesma forma, este será benéfico para o paciente, dado que se pretende tornar o processo de extração menos moroso, menos doloroso e acima de tudo com um menor número de danos na cavidade oral do mesmo. Este projeto é constituído por diferentes fases, destacando-se o estudo da anatomia oral bem como todo o processo inerente à extração dentária, a análise dos dispositivos que foram surgindo nesta área e o corpo do projeto, onde são apresentados todos os passos realizados para chegar a uma solução capaz de cumprir com os objetivos delineados. O culminar deste trabalho é a apresentação do modelo computacional do dispositivo, realizado recorrendo ao software AutoCAD 2000.
This project’s main goal was the study and design of a new medical device to assist dentists during the process of dental extraction. The aim was to develop a useful, efficient and safe dental instrument, both for the specialist and the patient. This comes up as a great innovation in this area, because it will allow the user to accomplish different tasks - to cut, to hold, to luxate and to extract - while using the same device. Besides that, it will also be possible to use it for different types of teeth, since the end tips can be changed according to each specific case. However, one of the strongest points of the device is the vibrating system that will assist the dentist to perform the dental luxation process, decreasing the efforts and necessary forces to be applied. In the same way, it will benefit the patient, making the extraction an easy and less painful that will also result in a smaller number of damages to the oral cavity. This project is structured in different sections, including the study of oral anatomy, as well as the whole process inherent to dental extraction, the analysis of devices that have been appearing in this area and the project’s itself, where every step to get to a solution capable to fulfill the preset goals is presented. The outcome of this work is the presentation of a computerized model of the instrument device, carried out with the AutoCAD 2000’s software.

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Books on the topic "LuxAB"

1

Elliot, H. M. Sir, 1808-1853. and Dowson John 1820-1881, eds. Muntakhab-ul lubab. Lahore: Sang-e-Meel Publications, 2006.

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al-Lubab: Syriac-Arabic dictionary. Piscataway, NJ: Gorgias Press, 2007.

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al-Aḥādīth al-wāridah fī al-luʻab wa-al-riyāḍah: Dirāsah ḥadīthīyah fiqhīyah. al-Dammām: Dār Ibn al-Jawzī lil-Nashr wa-al-Tawzīʻ, 2014.

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Imām, Ḥamādah. al-Ikhwān al-Muslimūn wa-al-Aqbāṭ min al-luʻab bi-al-dīn ilá al-luʻab bi-al-waṭan: Niṣf qarn min alāʻīb al-mukhābarāt al-Amrīkīyah maʻa al-mutaṭarrifīn al-Islāmīyīn wa-al-Masīḥīyīn. Imbābah [Giza]: Ḥiwār lil-Nashr wa-al-Tawzīʻ, 2007.

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Visita a las encomiendas de Indios de Córdoba 1692-1693: Transcripción y estudios sobre la visita de Antonio Martines Luxan de Vargas. Córdoba: Centro de Estudios Históricos "Prof. Carlos S.A. Segreti", 2009.

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Tihrani, Muhammad Husayn Husayni. Kernel of the kernel: Concerning the wayfaring and spiritual journey of the people of intellect : (Risala-yi Lubb al-lubab dar sayr wa suluk-i ulu'l albab) [sic] : a Shi'i approach to Sufism. Albany, NY: State University of New York Press, 2003.

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Fodors-Belgm & Luxb'88. Fodor's, 1987.

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Al Lubab Fi Tahdhib Al Ansab (1/2). Dar Al Ktub Al A'lmia, 2000.

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Lubab al-muhassal fi usul al-din (Maktabah al-falsafiyah). Dar al-Mashriq, 1995.

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Zarif, Muhammad Mustaqim Mohd. Peranan lubab al-Hadith dalam karya hadis di alam Melayu. Lubab al-hadith, 2014.

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Book chapters on the topic "LuxAB"

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Laakmann, Kathy. "Luxar 10.6 μm CO2 LX-20 Laser and Luxar Extend Systems for Adaptation to Other 10.6 μm CO2 Lasers for Arthroscopic Laser Surgery." In Arthroscopic Laser Surgery, 105–9. New York, NY: Springer New York, 1995. http://dx.doi.org/10.1007/978-1-4612-2468-6_18.

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"CHAPTER 11The Luxar Laser System." In Lasers in Aesthetic Surgery, edited by Gregory S. Keller, Patrick K. Lee, Victor G. Lacombe, James P. Watson, and Kenneth M. Toft. Stuttgart: Georg Thieme Verlag, 2001. http://dx.doi.org/10.1055/b-0034-50374.

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"Gabriel de Luxan to the House of Trade, Havana, June 29, 1586." In Further English Voyages to Spanish America, 1583-1594, 273. Hakluyt Society, 2017. http://dx.doi.org/10.4324/9781315583693-51.

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"Gabriel de Luxan and Diego Fernández de Quiñones to the Crown, Havana, June 28, 1586." In Further English Voyages to Spanish America, 1583-1594, 264–65. Hakluyt Society, 2017. http://dx.doi.org/10.4324/9781315583693-46.

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"Gabriel de Luxan and Diego Fernández de Quiñones to the Crown, Havana, July 1, 1586." In Further English Voyages to Spanish America, 1583-1594, 280–81. Hakluyt Society, 2017. http://dx.doi.org/10.4324/9781315583693-54.

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Conference papers on the topic "LuxAB"

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Arapov, Yu D., S. G. Grechin, and I. V. Kasyanov. "LuAB crystal for frequency conversion." In 2016 International Conference Laser Optics (LO). IEEE, 2016. http://dx.doi.org/10.1109/lo.2016.7549894.

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