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Dissertations / Theses on the topic 'M-cels'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Tyrer, Peter Charles, and n/a. "Targeting M-cells for oral vaccine delivery." University of Canberra. Health Sciences, 2004. http://erl.canberra.edu.au./public/adt-AUC20060427.122012.

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An in vitro model of the follicle-associated epithelia that overlie the Peyer's patches of the small intestine was developed and validated to examine the mechanisms of mucosal antigen sampling. This model displays many phenotypic and physiological characteristics of M cells including apical expression of [alpha]5[beta]l integrin and enhanced energy dependent participate transport. CD4+ T-cells were shown to be an important influence on the development of Mlike cells. The model was used to examine the M cell mediated uptake of several putative whole-cell killed bacterial vaccines. Greater numbe

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2

Blakemore, Louise Margaret. "Curcumin-induced G2/M cell cycle arrest in colorectal cancer cells." Thesis, University of Leicester, 2011. http://hdl.handle.net/2381/9809.

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Curcumin, a diet-derived chemopreventive and chemotherapeutic agent has been shown to induce G2/M cell cycle arrest, and previous studies suggested that microtubule depolymerisation may be linked to M-phase arrest. However, mechanisms involved have not been elucidated and often non-physiological concentrations of curcumin were used. The goal of this study was to characterise in more detail curcumin-induced cell cycle arrest using a panel of human colorectal cancer cell (CRC) lines, HT-29, SW480, HCT116 p53+/+, HCT116 p53-/- and HCT116 p21-/-. Using physiologically relevant concentrations of cu

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3

Mairopoulos, Dimitrios. "M-Cell assembly." Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/99294.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Architecture, June 2015.<br>Cataloged from PDF version of thesis. "June 2015."<br>Includes bibliographical references (page 47).<br>In this thesis I propose a self- assembly procedure called the Morphocell(M-Cell) assembly. This procedure is based on an assembly unit called the M-Cell. The M-Cell is comprised out of two components, the M-Block and the M-Clay (in which the M-Block is embedded). During the assembly procedure the M-Clay acts as the environment of the assembly for the M-Blocks. This allows a global, parallel assemb

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4

Mason, Caroline Margaret. "Characterisation of intestinal M cells using monoclonal antibodies." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260952.

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5

Sehgal, Anuj. "Investigating the development and function of M cells." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28738.

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Gut-associated lymphoid tissues such as Peyer’s patches (PP) are inductive sites for immune response in the intestine. Unlike other peripheral lymphoid tissues, gut-associated lymphoid tissues lack afferent lymphatics and can directly sample mucosal antigens by specialized epithelial cells in the follicular associated epithelia (FAE), known as M cells. M cells derive from Lgr5+ intestinal stem cells in intestinal crypts, where the daughter cells of Lgr5+ cells differentiate into M cells after stimulation from the cytokine receptor activator of nuclear factor-κB ligand (RANKL). RANKL is produce

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6

Schäkel, Knut, Claudia Poppe, Elfriede Mayer, Christine Federle, Gert Riethmüller, and Ernst Peter Rieber. "M-DC8+ Leukocytes – A Novel Human Dendritic Cell Population." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-135252.

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Dendritic cells (DC) constitute a heterogeneous leukocyte population having in common a unique capacity to induce primary T cell responses and are therefore most attractive candidates for immunomodulatory strategies. Two populations of blood DC (CD11c+ CD123dim and CD11c– CD123high) have been defined so far. However, their direct isolation for experimental purposes is hampered by their low frequency and by the lack of selective markers allowing large scale purification from blood. Here we describe the monoclonal antibody (mAb) M-DC8, which was generated by immunizing mice with highly enriched

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7

Baker, Peter Kenneth. "The role of M-CSF in hairy-cell leukaemia." Thesis, University of Liverpool, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367653.

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8

Sansom, Nigel P. "Antigen sampling by porcine intestinal Peyer's patch M-cells." Thesis, University of Aberdeen, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322637.

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9

Schäkel, Knut, Claudia Poppe, Elfriede Mayer, Christine Federle, Gert Riethmüller, and Ernst Peter Rieber. "M-DC8+ Leukocytes – A Novel Human Dendritic Cell Population." Karger, 1999. https://tud.qucosa.de/id/qucosa%3A27632.

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Dendritic cells (DC) constitute a heterogeneous leukocyte population having in common a unique capacity to induce primary T cell responses and are therefore most attractive candidates for immunomodulatory strategies. Two populations of blood DC (CD11c+ CD123dim and CD11c– CD123high) have been defined so far. However, their direct isolation for experimental purposes is hampered by their low frequency and by the lack of selective markers allowing large scale purification from blood. Here we describe the monoclonal antibody (mAb) M-DC8, which was generated by immunizing mice with highly enriched

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10

Messerly, Erin. "M-CSF Stress-Induced Priming of the Macrophage Cell." Ohio Dominican University Honors Theses / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1449484244.

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11

Frick, Florian Verfasser], John M. [Akademischer Betreuer] Sullivan, and Imre [Akademischer Betreuer] [Bárány. "Combinatorial restrictions on cell complexes / Florian Frick. Gutachter: Imre Bárány ; John M. Sullivan. Betreuer: John M. Sullivan." Berlin : Technische Universität Berlin, 2015. http://d-nb.info/1076082246/34.

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12

Fowler, Daniel William. "The anti-tumour immune responses of γδ T cells activated with BCG, M. vaccae and M. obuense". Thesis, St George's, University of London, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559387.

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Introduction: BCG and heat-killed M. vaccae and M. obuense are currently being used as cancer immunotherapeutic agents; however, the induced immune response is poorly characterised and potential biomarkers of response ill-defined. γδ T cells have recently emerged as key players in immunity against both bacterial infections and malignant transformations, yet their role in cancer immunotherapies such as BCG, M. vaccae and M. obuense has been largely over-looked. This project investigates whether these three bacterial preparations can elicit anti-tumour responses in human peripheral blood γδ T ce

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13

Frick, Florian [Verfasser], John M. Akademischer Betreuer] Sullivan, and Imre [Akademischer Betreuer] [Bárány. "Combinatorial restrictions on cell complexes / Florian Frick. Gutachter: Imre Bárány ; John M. Sullivan. Betreuer: John M. Sullivan." Berlin : Technische Universität Berlin, 2015. http://d-nb.info/1076082246/34.

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14

Petris, Carisa Kay. "Identification and characterization of M cells in the mammalian conjunctiva." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4888.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on December 12, 2007) Vita. Includes bibliographical references.

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15

Mcshane, Helen Irene. "Immunisation strategies for enhancing T cell responses against M. tuberculosis." Thesis, University of London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271722.

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16

Montanez-Wiscovich, Marjorie E. "Discerning the Role of LMO4 as a Global Modulator of G2/M Cell Cycle Progression and Centrosome Cycle in Breast Cancer Cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1259899890.

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17

LeaÌƒo, Maria JoaÌƒo de Lemos Pinto Estrela. "Modulation of G2/M cell cycle checkpoints by Epstein-Barr virus." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414473.

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18

CHEN, WENDY YUNTIEN. "SYNTHESIS AND OLIGOSACCHARIDE PROCESSING OF IMMUNOGLOBULIN-M DURING B-CELL DIFFERENTIATION." Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184207.

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In order to understand glycoprotein biosynthesis and processing, we have studied the glycosylation and intracellular assembly kinetics of murine IgM which are expressed functionally only at specific stages of B-cell differentiation by the corresponding tumor cell lines. We have shown that the majority of carbohydrate chains on intracellular IgM contain predominantly Man₈GlcNac₂ rate limiting step in the carbohydrate processing is the transport from the RER to the Golgi apparatus. We made comparisons of carbohydrate structures on secretory and membrane-bound u chains produced by different cell

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19

Quinn, Jennifer E. "BRCA1 mediated G2/M cell cycle arrest in response to taxol." Thesis, Queen's University Belfast, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326034.

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20

Gunn, Lena Elizabeth. "Validation of the M-Vac cell collection system for forensic purposes." Thesis, Boston University, 2013. https://hdl.handle.net/2144/21161.

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Thesis (M.S.F.S.)<br>There is need for further development of cellular collection techniques in the field of forensic science. Currently, forensic analysts are limited to the use of swabs, taping, cutting, and scraping methods to collect cellular material. Each of these methods has its own benefits and drawbacks, however, none of them result in 100% recovery of the cells. The Microbial Vacuum system (M-Vac®), developed by MSI, is a liquid based cellular collection system that was originally developed to collect microbes in the food-processing industry from various surfaces. This research re

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21

Samaraweera, Preminda. "Oligosaccharides of mouse immunoglobulin-M: Structural variations in hybridoma and myeloma cells." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184496.

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Many protein-linked oligosaccharides are believed to impart biological specificities to the molecules. The knowledge of detailed structural characteristics of oligosaccharides is essential for understanding their functions. In order to develop methodology for characterization of oligosaccharides of glycoproteins, and to compare glycosylation patterns of different immunoglobulins, oligosaccharides of IgM from two cell lines, MOPC 104E and PC 700, were analyzed. Homogeneous preparations of glycopeptides carrying individual glycosylation sites of the heavy chain were obtained from the two IgM's.

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22

Hirabayashi, Shigeki. "APOBEC3B is preferentially expressed at the G2/M phase of cell cycle." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/264663.

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京都大学<br>新制・課程博士<br>博士(医学)<br>甲第23382号<br>医博第4751号<br>新制||医||1052(附属図書館)<br>京都大学大学院医学研究科医学専攻<br>(主査)教授伊藤貴浩, 教授滝田順子, 教授江藤浩之<br>学位規則第4条第1項該当<br>Doctor of Medical Science<br>Kyoto University<br>DFAM

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23

Alsadoon, Hamadah S. "Use of Cell Phones in Education at King Saud University in the Kingdom of Saudi Arabia." Ohio University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1353509728.

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24

Roberts, Carol Louise. "The interaction of adherent invasive escherichia coli with intestinal epithelial cells, in vitro derived M-cells and macrophages." Thesis, University of Liverpool, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501692.

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The initial lesions observed in Crohn's disease typically occur over lymphoid aggregates in tne large bowel and Heyers patches in the small bowel where M-cells are present. An increased presence of Adherent and Invasive Escherichia coli (AlEC) has been reported in Crohn's disease mucosa. Crohn's disease is common in westernised countries where low levels of soluble fibre are eaten and where the use of emulsifiers in processed food is common. It is possible that dietary factors may be implicated in the pathogenesis of the disease. The aims of this work were to establish if AIEC interact with in

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25

Pröller, Stephan [Verfasser], Eva M. [Akademischer Betreuer] Herzig, Peter [Gutachter] Müller-Buschbaum, and Eva M. [Gutachter] Herzig. "Morphology Formation and Manipulation in Printed Organic Solar Cells / Stephan Pröller ; Gutachter: Peter Müller-Buschbaum, Eva M. Herzig ; Betreuer: Eva M. Herzig." München : Universitätsbibliothek der TU München, 2018. http://d-nb.info/1155303512/34.

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26

Pröller, Stephan Verfasser], Eva M. [Akademischer Betreuer] [Herzig, Peter [Gutachter] Müller-Buschbaum, and Eva M. [Gutachter] Herzig. "Morphology Formation and Manipulation in Printed Organic Solar Cells / Stephan Pröller ; Gutachter: Peter Müller-Buschbaum, Eva M. Herzig ; Betreuer: Eva M. Herzig." München : Universitätsbibliothek der TU München, 2018. http://d-nb.info/1155303512/34.

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27

Schwingel, Melanie [Verfasser], and M. [Akademischer Betreuer] Bastmeyer. "Multiple trap optical tweezers for cell force measurements / Melanie Schwingel. Betreuer: M. Bastmeyer." Karlsruhe : KIT-Bibliothek, 2012. http://d-nb.info/1023081792/34.

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28

Brearley, Madelaine C. "Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M/PCK2), phosphoglycerate dehydrogenase (PHGDH) and muscle cell growth." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/52138/.

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Our group reported upregulation of a novel group of genes was associated with beta-adrenergic agonist (BA)-induced muscle hypertrophy in pigs. The aim of this PhD was to investigate the expression of these genes, and particularly the role of mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M/PCK2) and phosphoglycerate dehydrogenase (PHGDH), in muscle cell growth. A significant (p < 0.01) increase in mRNA transcript abundance was detected at day 2 of differentiation in C2C12 cells for PEPCK-M, PHGDH, phosphoserine aminotransferase-1, phosphoserine phosphatase, asparagine synthetase, sestr

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29

Bryson, Benjamin Levi. "The Paradoxical Roles of Oncostatin M in Mammary Epithelial Cell Senescence and Transformation." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1510584483133814.

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30

Smigiel, Jacob. "ONCOSTATIN M & TRANSFORMING GROWTH FACTOR SIGNALING CONVERGE TO REGULATE CANCER CELL PLASTICITY." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case152891618991579.

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31

Managh, Amy J. "Single-cell tracking of therapeutic cells using Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry." Thesis, Loughborough University, 2014. https://dspace.lboro.ac.uk/2134/16723.

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Cellular therapy is emerging as a clinically viable strategy in the field of solid organ transplantation, where it is expected to reduce the dependency on conventional immunosuppression. This has produced a demand for highly sensitive methods to monitor the persistence and tissue distribution of administered cells in vivo. However, tracking cells presents significant challenges. In many cases transplanted cells are autologous with the immune system of the transplant recipient, and hence are invisible to typical methods of detection. To enable their differentiation, the cells must be labelled w

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32

Mei, Lin. "The M(1) muscarinic receptor and its second messenger coupling in human neuroblastoma cells and transfected murine fibroblast cells." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184759.

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The data of this study indicate that pirenzepine(PZ)-high affinity muscarinic receptors (mAChRs) are coupled to the hydrolysis of inositol lipids and not to the adenylate cyclase system in human neuroblastoma SH-SY5Y cells. The maximal carbachol(CCh)-stimulated [³H]IP₁ accumulation in the SH-SY5Y cells was decreased in the presence of 1 μg/ml pertussis toxin, suggesting that a pertussis toxin sensitive G-protein may be involved in the coupling. Several cell clones which express only M₁ mAChR were generated by transfecting the murine fibroblast B82 cells with the cloned rat genomic m₁ gene. The

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33

VAZQUEZ, MORENO LUZ. "BIOCHEMICAL AND GENETIC STUDIES OF ANTIBODY (IMMUNOGLOBULIN-M) PRODUCING CELLS (GLYCOPROTEINS, U-CHAIN, HYBRIDOMAS)." Diss., The University of Arizona, 1985. http://hdl.handle.net/10150/187920.

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We have chosen the murine immunoglobulin M (IgM) as system to study glycoprotein biosynthesis and carbohydrate processing. Secreted IgM heavy chain (m) has five glycosylation sites which location and structures have been determined. m chain variable region (VH) is involved in antigen binding, while the constant region (CH) is responsible for the effector functions in which the carbohydrate plays an important role. We have determined the carbohydrate structures present at each glycosylation site of IgM produced by a hybridoma cell line (PC 700) and its derived mutants and compared them to IgM f

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34

Brennecke, Anne-M. [Verfasser], and Gerhard [Akademischer Betreuer] Gross. "Kinetics of B cell development in adult mice / Anne-M. Brennecke ; Betreuer: Gerhard Gross." Braunschweig : Technische Universität Braunschweig, 2014. http://d-nb.info/1175821411/34.

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35

Cervigni, Romina Ines. "Analysis of the molecular mechanisms of the Golgi-based G2/M cell cycle checkpoint." Thesis, Open University, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580685.

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This thesis is focused on the role of Golgi fragmentation in the regulation of the G2/M transition of the cell cycle, and it is based on previous findings that Golgi fragmentation is required to enter into mitosis. The Golgi complex is composed of many cisternal stacks that are interconnected by tubules, to form a continuous 'ribbon-like' structure. During mitosis, the Golgi ribbon undergoes extensive fragmentation through a multi stage process that promotes its correct partitioning and inheritance by the daughter cells. The first part of my work is focused on the understanding of the mechanis

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36

Weber, Patrick [Verfasser], M. Cristina [Akademischer Betreuer] Cardoso, and Bodo [Akademischer Betreuer] Laube. "DNA replication dynamics in embryonic stem cells / Patrick Weber ; M. Cristina Cardoso, Bodo Laube." Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2018. http://d-nb.info/1171988176/34.

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Nassra, Merian. "Effets anti-inflammatoires des stilbènes sur des cultures cellulaires de microglies et mécanismes d'action." Thesis, Bordeaux 2, 2012. http://www.theses.fr/2012BOR22018/document.

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Les processus neuro-inflammatoires sont observés dans de nombreux troubles neurodégénératifs. Les cellules microgliales étant les principales cellules immunitaires du système nerveux central, plusieurs recherches ont été menées afin de déterminer des molécules possédant des propriétés anti-inflammatoires au niveau de ces cellules. Une famille de polyphénols, les stilbènes (des dérivés du resvératrol), présentent des activités anti-inflammatoires au niveau périphérique et central. Dans notre étude, nous avons premièrement évalué les propriétés anti-inflammatoires de 25 stilbènes en déterminant

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Ghai, Kanika. "Notch-Signaling in Retinal Regeneration and Müller glial Plasticity." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1256322124.

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Thanasoula, Maria. "ATM/ATR-dependent responses to dysfunctional telomeres at the G2/M transition." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:b9f806e3-88e5-4dc4-b2e9-8ecf854249d1.

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Mammalian telomeres are nucleoprotein complexes at the end of chromosomes containing a specific protein complex, called shelterin. Shelterin protects chromosome ends from the DNA damage response (DDR), by facilitating the formation of a telomeric capping structure, called the T-loop. During their elongation in S phase, telomeres become transiently uncapped and can be sensed as DNA damage in G2 phase. This leads to the recruitment of DDR factors, such as phosphorylated histone H2AX (γH2AX), to the telomeres forming the so-called, telomere dysfunction-induced foci (TIFs). My PhD work described h

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40

Rytkönen, J. (Jani). "Effect of heat denaturation of bovine milk beta-lactoglobulin on its epithelial transport and allergenicity." Doctoral thesis, University of Oulu, 2006. http://urn.fi/urn:isbn:9514281209.

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Abstract Beta-lactoglobulin (β-lg) is the main whey protein in bovine milk. It belongs to the lipocalin protein family, and it is one of the main milk allergens. Resistance to hydrolysis is a particular feature of β-lg making it possible that β-lg reaches the small intestine in its native form. Heat treatments during milk processing may change the native structure of bovine β-lg and change its intestinal transport properties. Heat induced conformational alterations may also expose new antigenic sites. However, there have been no previous studies on the effects of heat treatment on the transpor

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Hadley, Jennifer Kathleen. "Identification of channels underlying the M-like potassium current in NG108-15 neuroblastoma-glioma cells." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393710.

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Konishi, Satoshi. "Directed induction of functional multi-ciliated cells in proximal airway epithelial spheroids from human pluripotent stem cells." Kyoto University, 2016. http://hdl.handle.net/2433/215418.

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Mumtaz, Imtiaz M. [Verfasser]. "Effects of immunosuppressive drugs and CD4+ T-cell depletion on plasma cell survival in lupus prone (NZB/W) mice / Imtiaz M. Mumtaz." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2009. http://d-nb.info/1023710315/34.

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Singh, Vibhuti [Verfasser], and M. [Akademischer Betreuer] Zöller. "Attending persistent T cell activation in alopecia areata : A therapeutic option / Vibhuti Singh. Betreuer: M. Zöller." Karlsruhe : KIT-Bibliothek, 2011. http://d-nb.info/1014279712/34.

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Czepukojc, Beate M. [Verfasser], and Alexandra K. [Akademischer Betreuer] Kiemer. "Investigations on IMP2-2 and Kupffer cells in steatohepatitis / Beate M. Czepukojc ; Betreuer: Alexandra K. Kiemer." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2019. http://d-nb.info/1199933023/34.

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Shokri, Beshr [Verfasser], and M. [Akademischer Betreuer] Grimm. "Expression of 1,25-Dihydroxyvitamin D3 receptor in oral squamous cell carcinoma / Beshr Shokri ; Betreuer: M. Grimm." Tübingen : Universitätsbibliothek Tübingen, 2018. http://d-nb.info/1199355887/34.

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Jones, Matthew Dunford. "Effects of radiation on the Gâ†2/M checkpoint in human tumour cells of differing radiosensitivities." Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387452.

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Hatayama, Hiroshi. "Progesterone Enhances Macrophage Colony-Stimulating Factor(M-CSF) Production in Human Endometrial Stromal Cells in vitro." Kyoto University, 1995. http://hdl.handle.net/2433/160724.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである<br>Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(医学)<br>甲第5973号<br>医博第1639号<br>新制||医||603(附属図書館)<br>UT51-95-D292<br>京都大学大学院医学研究科外科系専攻<br>(主査)教授西川伸一, 教授塩田浩平, 教授森崇英<br>学位規則第4条第1項該当

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Kieswetter, Nathan Scott. "Remodelling of Mycobacterial Peptidoglycan During Cell Division and the Epigenetics of Macrophages during M. tuberculosis infection." Doctoral thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33815.

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There are so many people who I would like to thank. If it takes a village to raise a child, it certainly takes a city to train a scientist. Firstly, I would like to thank my supervisors, A/Prof Reto Guler, Prof Frank Brombacher and Dr Mumin Ozturk for allowing me to further my studies and allowing me to work on several interesting projects. Specifically, I would like to take this opportunity to thank A/Prof Reto Guler for his insightful patient advice, training and ever willingness to talk about my work. His brilliant example has made me a better scientist. Further, I would also like to thank

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Beckta, Jason. "ATM, BRCA1, and Aurora A: Mechanisms of G2/M Checkpoint Control in Human Embryonic Stem Cells." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3477.

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Abstract:

When cultured in vitro, human embryonic stem cells (hESCs) acquire genetic abnormalities that have slowed their therapeutic use. As hESCs have a “leaky” G1/S boundary, the pressure of ensuring genetic integrity falls on the G2/M checkpoint, which can be activated by failed chromosomal decatenation (among other stimuli). It is hypothesized that hESCs have a deficient decatenation checkpoint, but little data supports this. Evidence suggests that the ataxia telangiectasia mutated (ATM) kinase controls the G2/M decatenation and DNA damage checkpoints, though previous reports are conflicting on thi

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