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Journal articles on the topic 'M-cels'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Mesquita, Hilda de Souza Lima, and Ana Júlia Fernandes. "Variação de curta escala temporal de bactérias, plcofltoplâncton e nanoheterótrofos na região de Ubatuba - SP, Brasil." Revista Brasileira de Oceanografia 44, no. 1 (1996): 47–56. http://dx.doi.org/10.1590/s1413-77391996000100005.

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A variação temporal da comunidade microbiana (bactérias, picofitoplâncton total e nanoheterótrofos) nas águas de Ubatuba (23°S 45°W) foi estudada durante um período de 7 dias (de 27/02 a 04/03/1988). As amostras foram obtidas na termoclina, duas vezes ao dia (na estôfa da maré baixa e da maré alta durante o período diurno. A densidade de nanoheterótrofos variou de 0,9 a 3,5 x 10³ cels m-1 apresentando valor médio de 2,3 x 10³ cels m-1. Picofitoplâncton total foi representado principalmente por cianobactérias cocóides e sua denside de variocões de 1,0 a 7,6 x 10(6) cels m-1. O número de bactéri

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Samper Prunera, Emili. "(Algunes) variacions sobre el diable: una proposta de catalogació de dues llegendes satàniques." Estudis de Literatura Oral Popular / Studies in Oral Folk Literature, no. 4 (December 17, 2015): 107. http://dx.doi.org/10.17345/elop2015107-120.

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En el marc del projecte «RondCat: rondalles catalanes», dirigit per Carme Oriol i Josep M. Pujol, i la catalogació de rondalles que no tenen correspondència a l’índex internacional d’Aarne-Thompson-Uther [ATU], es proposa la creació de dos tipus nous: C-103 («Dimoni, on vas?») i C-113 (La fondària del gorg). El punt de partida és l’estudi de la llegenda satànica fet per Josep M. Pujol l’any 1994 («Variacions sobre el diable»), així com el corpus recollit pel folklorista Cels Gomis i Mestre (1841-1915). La proposta de creació dels dos tipus inclou la relació de les versions catalanes localitzad

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3

Magalhaes, Roberto J. Pessoa, María-Belén Vidriales, Bruno Paiva, et al. "Multidimensional Flow Cytometric (MFC) Analysis of the Immune System of Multiple Myeloma (MM) Patients Achieving Long Term Disease Control." Blood 118, no. 21 (2011): 810. http://dx.doi.org/10.1182/blood.v118.21.810.810.

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Abstract Abstract 810FN2 Increasing evidence shows that a small fraction of MM patients (pts) treated with high-dose therapy followed by autologous stem cell transplantation achieve long-term remission. Interestingly, this is not restricted to pts in complete response (CR), since those that revert to a monoclonal gammopathy of undetermined significance (MGUS) profile may also achieve long-term remission, despite the persistence of residual myeloma plasma cells (PCs). These results suggest that in addition to the anti-myeloma therapy, other factors may play a role in the control of the disease.

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4

Setlow, Jane K. "Genetics in Chinese Hamster Cells Molecular Cell Genetics M. M. Gottesman." BioScience 36, no. 10 (1986): 680–82. http://dx.doi.org/10.2307/1310394.

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5

Kanaya, Takashi, Kohtaro Miyazawa, Ikuro Takakura, et al. "Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage." American Journal of Physiology-Gastrointestinal and Liver Physiology 295, no. 2 (2008): G273—G284. http://dx.doi.org/10.1152/ajpgi.00378.2007.

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M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal e

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Uemura, N., K. Ozawa, K. Takahashi, et al. "Binding of membrane-anchored macrophage colony-stimulating factor (M- CSF) to its receptor mediates specific adhesion between stromal cells and M-CSF receptor-bearing hematopoietic cells." Blood 82, no. 9 (1993): 2634–40. http://dx.doi.org/10.1182/blood.v82.9.2634.2634.

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Abstract To explore the biologic significance of the membrane-anchored form of macrophage colony-stimulating factor (M-CSF), we examined whether interaction between membrane-bound M-CSF and its receptor mediates intercellular adhesion as well as cell proliferation and differentiation. Human M-CSF receptors were expressed on a murine interleukin-2 (IL-3)-dependent cell line, FDC-P2, by DNA transfection with the c-fms gene (FDC-P2-MCSFR). A human bone marrow-derived stromal cell line, KM102, was used in the cell adhesion assay. The expression of membrane-bound M-CSF on KM102 cells was demonstrat

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Uemura, N., K. Ozawa, K. Takahashi, et al. "Binding of membrane-anchored macrophage colony-stimulating factor (M- CSF) to its receptor mediates specific adhesion between stromal cells and M-CSF receptor-bearing hematopoietic cells." Blood 82, no. 9 (1993): 2634–40. http://dx.doi.org/10.1182/blood.v82.9.2634.bloodjournal8292634.

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To explore the biologic significance of the membrane-anchored form of macrophage colony-stimulating factor (M-CSF), we examined whether interaction between membrane-bound M-CSF and its receptor mediates intercellular adhesion as well as cell proliferation and differentiation. Human M-CSF receptors were expressed on a murine interleukin-2 (IL-3)-dependent cell line, FDC-P2, by DNA transfection with the c-fms gene (FDC-P2-MCSFR). A human bone marrow-derived stromal cell line, KM102, was used in the cell adhesion assay. The expression of membrane-bound M-CSF on KM102 cells was demonstrated by flo

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8

Ebisawa, Masashi, Koji Hase, Daisuke Takahashi, et al. "CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch." International Immunology 23, no. 4 (2011): 261–69. http://dx.doi.org/10.1093/intimm/dxq478.

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9

Lelouard, Hugues, Alain Sahuquet, Hubert Reggio, and Philippe Montcourrier. "Rabbit M cells and dome enterocytes are distinct cell lineages." Journal of Cell Science 114, no. 11 (2001): 2077–83. http://dx.doi.org/10.1242/jcs.114.11.2077.

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We have studied the M cell origin and differentiation pathway in rabbit gut-associated lymphoid tissues. Micro-dissected domes and epithelium isolated by ethylene diamine tetra acetic acid detachment allowed us to view the whole epithelial surface from the bottom of crypts to the top of domes. We used monoclonal antibodies specific to the apex of either M cells or dome enterocytes, lectins, and antibodies to vimentin in appendix, distal Peyer’s patches and caecal patches. The earliest vimentin-labeled M cells were observed in the BrdU-positive proliferative zone of dome-associated crypts. Grad

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Klisuric, Ana, Benjamin Thierry, Ludivine Delon, Clive A. Prestidge, and Rachel J. Gibson. "Identifying human and murine M cells in vitro." Experimental Biology and Medicine 244, no. 7 (2019): 554–64. http://dx.doi.org/10.1177/1535370219838674.

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M cells are an epithelial cell population found in the follicle-associated epithelium overlying gut-associated lymphoid tissues. They are specialized in the transcytosis of luminal antigens. Their transcytotic capacity and location in an immunocompetent environment has prompted the study of these cells as possible targets for oral drug delivery systems. Currently, the models most commonly used to study M cells are restricted to in vivo experiments conducted in mice, and in vitro studies conducted in models comprised either of primary epithelial cells or established cell lines of murine or huma

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11

Ohno, Hiroshi. "Intestinal M cells." Journal of Biochemistry 159, no. 2 (2015): 151–60. http://dx.doi.org/10.1093/jb/mvv121.

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12

ANTZELEVITCH, CHARLES, WATARU SHIMIZU, GAN-XIN YAN, et al. "The M Cell:." Journal of Cardiovascular Electrophysiology 10, no. 8 (1999): 1124–52. http://dx.doi.org/10.1111/j.1540-8167.1999.tb00287.x.

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13

Antzelevitch, Charles. "The M Cell." Journal of Cardiovascular Pharmacology and Therapeutics 2, no. 1 (1997): 73–76. http://dx.doi.org/10.1177/107424849700200109.

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14

George, Joel Johnson, Laura Martin-Diaz, Markus J. T. Ojanen, Rosa Gasa, Marko Pesu, and Keijo Viiri. "PRC2 Regulated Atoh8 Is a Regulator of Intestinal Microfold Cell (M Cell) Differentiation." International Journal of Molecular Sciences 22, no. 17 (2021): 9355. http://dx.doi.org/10.3390/ijms22179355.

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Intestinal microfold cells (M cells) are a dynamic lineage of epithelial cells that initiate mucosal immunity in the intestine. They are responsible for the uptake and transcytosis of microorganisms, pathogens, and other antigens in the gastrointestinal tract. A mature M cell expresses a receptor Gp2 which binds to pathogens and aids in the uptake. Due to the rarity of these cells in the intestine, their development and differentiation remain yet to be fully understood. We recently demonstrated that polycomb repressive complex 2 (PRC2) is an epigenetic regulator of M cell development, and 12 n

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15

Reyes, Leticia, Maureen K. Davidson, Linda C. Thomas, and Jerry K. Davis. "Effects of Mycoplasma fermentansincognitus on Differentiation of THP-1 Cells." Infection and Immunity 67, no. 7 (1999): 3188–92. http://dx.doi.org/10.1128/iai.67.7.3188-3192.1999.

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ABSTRACT Mycoplasma fermentans incognitus has been isolated from human tissue in patients both with and without AIDS who died of systemic infection. M. fermentans incognitus and other strains of M. fermentans have been associated with rheumatoid arthritis. While cell extracts of M. fermentansincognitus can induce changes in murine and human cells of the monocytic lineage, little is known about interactions of viable organisms with such cells. Because of the central role of macrophages in chronic inflammation, we examined the effects of M. fermentans incognitus on surface markers and functions

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Clark, M. Ann, Barry H. Hirst та Mark A. Jepson. "M-Cell Surface β1 Integrin Expression and Invasin-Mediated Targeting of Yersinia pseudotuberculosis to Mouse Peyer’s Patch M Cells". Infection and Immunity 66, № 3 (1998): 1237–43. http://dx.doi.org/10.1128/iai.66.3.1237-1243.1998.

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ABSTRACT Quantitative analysis of Yersinia pseudotuberculosisinfection of murine gut loops revealed that significantly more wild-type bacteria associated with Peyer’s patch M cells than with dome enterocytes or goblet cells. An invasin-deficient mutant was significantly attenuated for M-cell invasion, while β1 integrin expression was demonstrated in the apical membranes of M cells but not enterocytes. M-cell targeting by Yersinia pseudotuberculosis in vivo may, therefore, be mediated primarily by the interaction of invasin with cell surface β1 integrins.

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Kitahashi, Takashi, Satoshi Ogawa, Tomoko Soga, Yasuo Sakuma, and Ishwar Parhar. "Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary." Endocrinology 148, no. 12 (2007): 5822–30. http://dx.doi.org/10.1210/en.2007-0311.

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The role of steroid/thyroid hormones in the regulation of endocrine cells at the level of the pituitary has remained unclear. Therefore, using single-cell quantitative real-time PCR, we examined absolute amounts of transcripts for nuclear receptors [estrogen receptors (ERs) α, β, and γ; androgen receptors (ARs) a and b; glucocorticoid receptors (GRs) 1, 2a, and 2b; and thyroid hormone receptors (TRs) α1, α2, and β] in pituitary cells of immature (IM) and mature (M) male tilapia, Oreochromis niloticus. In the two reproductive stages, ACTH cells expressed only ERβ, whereas all other pituitary ce

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18

Gebert, A. "M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes." Journal of Histochemistry & Cytochemistry 44, no. 9 (1996): 1033–42. http://dx.doi.org/10.1177/44.9.8773569.

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The tonsil crypt epithelium contains membranous (M)-cells that transport antigens from the lumen to underlying lymphoid cells, thereby initiating specific immune responses. Mechanisms mediating the adhesion of antigens to the M-cell surface are important for effective and selective uptake of potential pathogens but are still poorly understood. Therefore, the carbohydrates present on crypt epithelial cells of the rabbit palatine tonsil were studied by lectin histochemistry. Ultrathin LR White sections were incubated with a panel of eight lectins conjugated to colloidal gold or biotin. The glyco

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Korbmacher, C., A. S. Segal, G. Fejes-Tóth, G. Giebisch, and E. L. Boulpaep. "Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells." Journal of General Physiology 102, no. 4 (1993): 761–93. http://dx.doi.org/10.1085/jgp.102.4.761.

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M-1 cells, derived from a microdissected cortical collecting duct of a transgenic mouse, grown to confluence on a permeable support, develop a lumen-negative amiloride-sensitive transepithelial potential, reabsorb sodium, and secrete potassium. Electron micrographs show morphological features typical of principal cells in vivo. Using the patch clamp technique distinct differences are detected in whole-cell membrane current and voltage (Vm) between single M-1 cells 24 h after seeding vs cells grown to confluence. (a) Under control conditions (pipette: KCl-Ringer; bath: NaCl-Ringer) Vm averages

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20

Clark, M. A., M. A. Jepson, N. L. Simmons, T. A. Booth, and B. H. Hirst. "Differential expression of lectin-binding sites defines mouse intestinal M-cells." Journal of Histochemistry & Cytochemistry 41, no. 11 (1993): 1679–87. http://dx.doi.org/10.1177/41.11.7691933.

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We investigated the binding of four lectins to the follicle-associated epithelium (FAE) overlying fixed mouse small intestinal Peyer's patches to identify M-cell-specific surface markers. Wheat germ agglutinin and peanut agglutinin displayed heterogeneous staining patterns, binding most avidly to the intestine goblet cells. In contrast, the lectins Ulex europaeus 1 (UEA 1) and Psophocarpus tetragonolobus (winged bean; WBA) were almost exclusively M-cell specific. When confocal laser scanning images of tissues stained with fluorescein isothiocyanate (FITC)-conjugated UEA1 or WBA were compared w

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Lee, Jinhee, Keumhwa Choi, Michael R. Olin, Sang-Nae Cho та Thomas W. Molitor. "γδ T Cells in Immunity Induced by Mycobacterium bovis Bacillus Calmette-Guérin Vaccination". Infection and Immunity 72, № 3 (2004): 1504–11. http://dx.doi.org/10.1128/iai.72.3.1504-1511.2004.

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ABSTRACT Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination is efficacious for newborns or adults with no previous exposure to environmental mycobacteria. To determine the relative contribution and the nature of γδ T-cell receptor-positive T cells in newborns, compared to CD4+ T cells, in immunity induced by M. bovis BCG vaccination, 4-week-old specific-pathogen-free pigs were vaccinated with M. bovis BCG and monitored by following the γδ T-cell immune responses. A flow cytometry-based proliferation assay and intracellular staining for gamma interferon (IFN-γ) were used to examine

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22

Jensen, V. Behrana, John T. Harty, and Bradley D. Jones. "Interactions of the Invasive PathogensSalmonella typhimurium, Listeria monocytogenes, and Shigella flexneri with M Cells and Murine Peyer’s Patches." Infection and Immunity 66, no. 8 (1998): 3758–66. http://dx.doi.org/10.1128/iai.66.8.3758-3766.1998.

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ABSTRACT Invasive enteric bacteria must pass through the intestinal epithelium in order to establish infection. It is becoming clear that a common target for intestinal mucosa penetration is the specialized epithelial cell of Peyer’s patches, the M cell. In order to gain a better understanding of how bacteria interact with M cells, we have compared the interactions of Salmonella typhimurium,Listeria monocytogenes, and Shigella flexneriwith M cells by using a murine ligated-loop model. Our results indicate that S. typhimurium possesses a highly efficient mechanism for M cell entry that targets

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Kanaya, Takashi, Sayuri Sakakibara, Toshi Jinnohara та ін. "Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling". Journal of Experimental Medicine 215, № 2 (2018): 501–19. http://dx.doi.org/10.1084/jem.20160659.

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M cells are located in the follicle-associated epithelium (FAE) that covers Peyer’s patches (PPs) and are responsible for the uptake of intestinal antigens. The differentiation of M cells is initiated by receptor activator of NF-κB. However, the intracellular pathways involved in M cell differentiation are still elusive. In this study, we demonstrate that the NF-κB pathway activated by RANK is essential for M cell differentiation using in vitro organoid culture. Overexpression of NF-κB transcription factors enhances the expression of M cell–associated molecules but is not sufficient to complet

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Larabi, Anaïs, Laurène Salesse, Charlotte Cordonnier, et al. "Differential miRNA-Gene Expression in M Cells in Response to Crohn’s Disease-Associated AIEC." Microorganisms 8, no. 8 (2020): 1205. http://dx.doi.org/10.3390/microorganisms8081205.

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Adherent-invasive Escherichia coli (AIEC), which abnormally colonize the ileal mucosa of Crohn’s disease (CD) patients, are able to invade intestinal epithelial cells (IECs) and translocate through M cells overlying Peyer’s patches. The levels of microRNA (miRNA) and gene expression in IECs and M cells upon AIEC infection have not been investigated. Here, we used human intestinal epithelial Caco-2 monolayers and an in vitro M-cell model of AIEC translocation to analyze comprehensive miRNA and gene profiling under basal condition and upon infection with the reference AIEC LF82 strain. Our resul

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Helander, Anna, Katherine J. Silvey, Nicholas J. Mantis та ін. "The Viral σ1 Protein and Glycoconjugates Containing α2-3-Linked Sialic Acid Are Involved in Type 1 Reovirus Adherence to M Cell Apical Surfaces". Journal of Virology 77, № 14 (2003): 7964–77. http://dx.doi.org/10.1128/jvi.77.14.7964-7977.2003.

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ABSTRACT Type 1 reoviruses invade the intestinal mucosa of mice by adhering selectively to M cells in the follicle-associated epithelium and then exploiting M cell transport activity. The purpose of this study was to identify the apical cell membrane component and viral protein that mediate the M cell adherence of these viruses. Virions and infectious subviral particles of reovirus type 1 Lang (T1L) adhered to rabbit M cells in Peyer's patch mucosal explants and to tissue sections in an overlay assay. Viral adherence was abolished by pretreatment of sections with periodate and in the presence

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Dale, J. B., and E. H. Beachey. "Human cytotoxic T lymphocytes evoked by group A streptococcal M proteins." Journal of Experimental Medicine 166, no. 6 (1987): 1825–35. http://dx.doi.org/10.1084/jem.166.6.1825.

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Purified group A streptococcal M proteins were used to stimulate peripheral blood lymphocytes from normal adult volunteers. The activated lymphocytes were cytotoxic against cultured human heart cells, as well as liver cells, fibroblasts, and K562 cells, but showed only minimal cytotoxicity against several animal cell types. The cytotoxic activity evoked by type 5 M protein was dose and time dependent. Rabbit antisera against pep M5 that contained heart-crossreactive antibodies partially inhibited cytotoxicity against heart cells, but had no effect on other target cells, suggesting that a fract

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Brzostek, Joanna, Amierah Fatin, Wen Hui Chua, Hui Yi Tan, Thomas Dick, and Nicholas R. J. Gascoigne. "Single Cell Analysis of Drug Susceptibility of Mycobacterium abscessus during Macrophage Infection." Antibiotics 9, no. 10 (2020): 711. http://dx.doi.org/10.3390/antibiotics9100711.

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Mycobacterium abscessus is an emerging health risk to immunocompromised individuals and to people with pre-existing pulmonary conditions. As M. abscessus possesses multiple mechanisms of drug resistance, treatments of M. abscessus are of poor efficacy. Therefore, there is an urgent need for new therapeutic strategies targeting M. abscessus. We describe an experimental system for screening of compounds for their antimicrobial activity against intracellular M. abscessus using flow cytometry and imaging flow cytometry. The assay allows simultaneous analysis of multiple parameters, such as proport

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Schimpel, Christa, Oliver Werzer, Eleonore Fröhlich, et al. "Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells." Beilstein Journal of Nanotechnology 6 (July 6, 2015): 1457–66. http://dx.doi.org/10.3762/bjnano.6.151.

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The small intestine is a complex system that carries out various functions. The main function of enterocytes is absorption of nutrients, whereas membranous cells (M cells) are responsible for delivering antigens/foreign substances to the mucosal lymphoid tissues. However, to get a fundamental understanding of how cellular structures contribute to physiological processes, precise knowledge about surface morphologies, cytoskeleton organizations and biomechanical properties is necessary. Atomic force microscopy (AFM) was used here as a powerful tool to study surface topographies of Caco-2 cells a

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Chiou, Pinwen P., Carol H. Kim, Patricia Ormonde, and Jo-Ann C. Leong. "Infectious Hematopoietic Necrosis Virus Matrix Protein Inhibits Host-Directed Gene Expression and Induces Morphological Changes of Apoptosis in Cell Cultures." Journal of Virology 74, no. 16 (2000): 7619–27. http://dx.doi.org/10.1128/jvi.74.16.7619-7627.2000.

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ABSTRACT Infectious hematopoietic necrosis virus (IHNV) infection in tissue culture cells has previously been shown to result in the shutdown of host protein synthesis, cell rounding, and cell death. We report here an investigation of the cytopathogenicity of the viral phosphoprotein (P or M1), matrix (M or M2), and nonvirion (NV) proteins in cultured fish cells. The expression of M alone potently inhibited reporter gene expression from a viral and an interferon (IFN)-inducible promoter, whereas P and NV did not produce a similar effect. Northern blot analysis further revealed a reduction in t

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Schmitz, Marc, Senming Zhao, Yvonne Deuse, Knut Schaekel, Martin Bornhaeuser, and Peter Rieber. "Tumoricidal Potential of Native Human Blood Dendritic Cells: Direct Tumor Cell Killing and Activation of NK Cell-Mediated Cytotoxicity." Blood 104, no. 11 (2004): 449. http://dx.doi.org/10.1182/blood.v104.11.449.449.

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Abstract Dendritic cells (DCs) are characterized by their unique capacity for primary T cell activation, providing the opportunity of DC-based cancer vaccination protocols. Recently, we defined a novel major subset of human blood DCs by using the monoclonal antibody M-DC8 which recognizes a carbohydrate modification of P selectin glycoprotein ligand-1 (PSGL-1) selectively expressed on these cells (Immunity2002;17:289-301). In addition to a marked capacity to activate tumor-reactive cytotoxic T cells M-DC8+ DCs efficiently mediate antibody-dependent cytotoxicity (Blood;2002;100:1502-1504). In t

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Wood, Megan B., Daniel Rios та Ifor R. Williams. "TNF-α augments RANKL-dependent intestinal M cell differentiation in enteroid cultures". American Journal of Physiology-Cell Physiology 311, № 3 (2016): C498—C507. http://dx.doi.org/10.1152/ajpcell.00108.2016.

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Microfold (M) cells are phagocytic intestinal epithelial cells in the follicle-associated epithelium of Peyer's patches that transport particulate antigens from the gut lumen into the subepithelial dome. Differentiation of M cells from epithelial stem cells in intestinal crypts requires the cytokine receptor activator of NF-κB ligand (RANKL) and the transcription factor Spi-B. We used three-dimensional enteroid cultures established with small intestinal crypts from mice as a model system to investigate signaling pathways involved in M cell differentiation and the influence of other cytokines o

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Abboud, SL, and M. Pinzani. "Peptide growth factors stimulate macrophage colony-stimulating factor in murine stromal cells." Blood 78, no. 1 (1991): 103–9. http://dx.doi.org/10.1182/blood.v78.1.103.103.

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Abstract Bone marrow stromal cells influence hematopoiesis through cell-cell interaction and release of hematopoietic growth factors. Macrophage colony-stimulating factor (M-CSF) is constitutively produced by several murine and human stromal cell lines and is induced by inflammatory mediators such as interleukin-1 alpha or tumor necrosis factor-alpha (TNF-alpha) in a variety of mesenchymal cells. Other potentially important regulatory molecules such as platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF), released by activated monocytes in response to inflammation, s

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Abboud, SL, and M. Pinzani. "Peptide growth factors stimulate macrophage colony-stimulating factor in murine stromal cells." Blood 78, no. 1 (1991): 103–9. http://dx.doi.org/10.1182/blood.v78.1.103.bloodjournal781103.

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Bone marrow stromal cells influence hematopoiesis through cell-cell interaction and release of hematopoietic growth factors. Macrophage colony-stimulating factor (M-CSF) is constitutively produced by several murine and human stromal cell lines and is induced by inflammatory mediators such as interleukin-1 alpha or tumor necrosis factor-alpha (TNF-alpha) in a variety of mesenchymal cells. Other potentially important regulatory molecules such as platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF), released by activated monocytes in response to inflammation, stimulate

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Ahmad, Aamir, Fazlul H. Sarkar, Main Maitah, et al. "Effect of GDC-0449, a hedgehog (Hh) pathway inhibitor, on tumor activity and on erlotinib and cisplatin in a xenograft model of non-small cell lung cancer (NSCLC) with activated Hh pathway." Journal of Clinical Oncology 30, no. 15_suppl (2012): e21057-e21057. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.e21057.

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e21057 Background: We have previously shown that chronic exposure of A549 NSCLC cell line to TGF-β1 can induce epithelial-mesenchymal transition (EMT) and can make these cell lines (A549-M) resistant to erlotinib, an EGFR inhibitor and cisplatin. EMT in A549-M cells was associated with significant activation of signaling through the Hh pathway. We therefore evaluated the activity of GDC-0449, an inhibitor of the Hh pathway, and assessed its activity in combination with erlotinib and cisplatin in A549-M cells both in vitro and in vivo. Methods: We assessed the effects of GDC-0449 (20 nM) on clo

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Krilis, SA, KF Austen, JL Macpherson, CF Nicodemus, MF Gurish, and RL Stevens. "Continuous release of secretory granule proteoglycans from a cell strain derived from the bone marrow of a patient with diffuse cutaneous mastocytosis." Blood 79, no. 1 (1992): 144–51. http://dx.doi.org/10.1182/blood.v79.1.144.144.

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Abstract A human cell strain (designated HBM-M) that was derived from the bone marrow of a child with diffuse cutaneous mastocytosis was previously found to possess features that suggested it belonged in the mast cell/monocyte lineage. HBM-M cells synthesized approximately 150-Kd Pronase-resistant proteoglycans that were recognized by an antihuman secretory granule proteoglycan peptide core antibody. These cells also contained in relatively high abundance the same sized mRNA transcript that encodes the peptide core of proteoglycans that are normally localized to secretory granules of hematopoi

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Krilis, SA, KF Austen, JL Macpherson, CF Nicodemus, MF Gurish, and RL Stevens. "Continuous release of secretory granule proteoglycans from a cell strain derived from the bone marrow of a patient with diffuse cutaneous mastocytosis." Blood 79, no. 1 (1992): 144–51. http://dx.doi.org/10.1182/blood.v79.1.144.bloodjournal791144.

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A human cell strain (designated HBM-M) that was derived from the bone marrow of a child with diffuse cutaneous mastocytosis was previously found to possess features that suggested it belonged in the mast cell/monocyte lineage. HBM-M cells synthesized approximately 150-Kd Pronase-resistant proteoglycans that were recognized by an antihuman secretory granule proteoglycan peptide core antibody. These cells also contained in relatively high abundance the same sized mRNA transcript that encodes the peptide core of proteoglycans that are normally localized to secretory granules of hematopoietic cell

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Kolomytkin, Oleg V., Andrew A. Marino, Kalia K. Sadasivan, Robert E. Wolf та James A. Albright. "Interleukin-1β switches electrophysiological states of synovial fibroblasts". American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 273, № 5 (1997): R1822—R1828. http://dx.doi.org/10.1152/ajpregu.1997.273.5.r1822.

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The role of electrophysiological events in signal transduction of interleukin-1β (IL-1β) was investigated in rabbit synovial fibroblasts using the perforated-patch method. Aggregated synovial fibroblasts occurred in two different electrophysiological states having membrane potentials ( V m) of −63 ± 4 ( n = 71) and −27 ± 10 mV ( n = 55) (high and low V m, respectively). IL-1β affected the cells with high V m; it switched the state of the cell from high to low V m. This effect was strongly dependent on the external potential applied to the cell membrane. Low V m(−30 mV) alone without IL-1β did

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Benardete, Ethan A., Ehud Kaplan, and Bruce W. Knight. "Contrast gain control in the primate retina: P cells are not X-like, some M cells are." Visual Neuroscience 8, no. 5 (1992): 483–86. http://dx.doi.org/10.1017/s0952523800004995.

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AbstractPrimate retinal ganglion cells that project to the magnocellular layers of the lateral geniculate nucleus (M) are much more sensitive to luminance contrast than those ganglion cells projecting to the parvocellular layers (P). We now report that increasing contrast modifies the temporal-frequency response of M cells, but not of P cells. With rising contrast, the M cells' responses to sinusoidal stimuli show an increasing attenuation at low temporal frequencies while the P cells' responses scale uniformly. The characteristic features of M-cell dynamics are well described by a model origi

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Zeschnigk, M., D. Kozian, C. Kuch, M. Schmoll, and A. Starzinski-Powitz. "Involvement of M-cadherin in terminal differentiation of skeletal muscle cells." Journal of Cell Science 108, no. 9 (1995): 2973–81. http://dx.doi.org/10.1242/jcs.108.9.2973.

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Cadherins are a gene family encoding calcium-dependent cell adhesion proteins which are thought to act in the establishment and maintenance of tissue organization. M-cadherin, one member of the family, has been found in myogenic cells of somitic origin during embryogenesis and in the adult. These findings have suggested that M-cadherin is involved in the regulation of morphogenesis of skeletal muscle cells. Therefore, we investigated the function of M-cadherin in the fusion of myoblasts into myotubes (terminal differentiation) in cell culture. Furthermore, we tested whether M-cadherin might in

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van der Merwe, Jacques, Tracy Prysliak, and Jose Perez-Casal. "Invasion of Bovine Peripheral Blood Mononuclear Cells and Erythrocytes by Mycoplasma bovis." Infection and Immunity 78, no. 11 (2010): 4570–78. http://dx.doi.org/10.1128/iai.00707-10.

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ABSTRACT Mycoplasma bovis is a small, cell wall-less bacterium that contributes to a number of chronic inflammatory diseases in both dairy and feedlot cattle, including mastitis and bronchopneumonia. Numerous reports have implicated M. bovis in the activation of the immune system, while at the same time inhibiting immune cell proliferation. However, it is unknown whether the specific immune-cell population M. bovis is capable of attaching to and potentially invading. Here, we demonstrate that incubation of M. bovis Mb1 with bovine peripheral blood mononuclear cells (PBMC) resulted in a signifi

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Wang, Lei, Brandon L. Walker, Devang Bhatt, Patrick J. Kennedy, and William T. Tse. "Clonal Dedifferentiation of Human Myogenic Progenitors into Skeletal Muscle Stem Cells Induced by MAPK Inhibition." Blood 110, no. 11 (2007): 3701. http://dx.doi.org/10.1182/blood.v110.11.3701.3701.

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Abstract Understanding the biology of skeletal muscle stem cells can facilitate development of effective cellular therapy for muscle diseases such as muscular dystrophy. While studying human bone marrow stromal cells, we identified a stromal cell subclone, WB15-M, which has developed spontaneously into a skeletal muscle cell line. This subclone no longer expressed CD105, CD90 and CD44, cell surface markers typically found on bone marrow stromal cells. Instead they expressed alpha7-integrin, an antigen found on myoblasts and regenerating muscles. WB15-M cells were positive for the myogenic regu

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Nair, Vidhya, Haaris Khan, Ron Mitchell, and Michael U. Shiloh. "Role of M Cells in Human Mucosal Immunity to Mycobacterium tuberculosis." Open Forum Infectious Diseases 4, suppl_1 (2017): S48. http://dx.doi.org/10.1093/ofid/ofx162.111.

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Abstract Background Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is a bacterial pathogen that infects roughly one-third of the worldÕs population and causes 1–2 million deaths per year. The current paradigm is that phagocytosis of Mtb by patrolling alveolar macrophages initiates Mtb infection. While this model can account for pulmonary TB, it does not adequately explain the occurrence of extrapulmonary forms of TB that manifest in the absence of obvious lung involvement, such as tuberculous cervical lymphadenitis, also known as scrofula. We hypothesized that spec

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Staub, F., A. Winkler, J. Peters, O. Kempski, V. Kachel, and A. Baethmann. "Swelling, Acidosis, and Irreversible Damage of Glial Cells from Exposure to Arachidonic Acid in vitro." Journal of Cerebral Blood Flow & Metabolism 14, no. 6 (1994): 1030–39. http://dx.doi.org/10.1038/jcbfm.1994.135.

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Swelling and damage of C6 glioma cells and of primary cultured astrocytes were analyzed in vitro during incubation with arachidonic acid (AA; 20:4). The cells were suspended in a physiological medium supplemented with AA at concentrations of 0.001–1.0 m M. Cell swelling was quantified by flow cytometry with hydrodynamic focusing. Flow cytometry was also utilized for assessment of cell viability by exclusion of the fluorescent dye propidium iodide and for measurement of the intracellular pH (pHi) by 2′,7′-bis-(2-carboxyethyl)−5(and −6)carboxyfluorescein. Administration of AA caused an immediate

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Moriuchi, Hiroyuki, Masako Moriuchi, and Anthony S. Fauci. "Differentiation of Promonocytic U937 Subclones into Macrophagelike Phenotypes Regulates a Cellular Factor(s) Which Modulates Fusion/Entry of Macrophagetropic Human Immunodeficiency Virus Type 1." Journal of Virology 72, no. 4 (1998): 3394–400. http://dx.doi.org/10.1128/jvi.72.4.3394-3400.1998.

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ABSTRACT Monocytes/macrophages (M/M) and CD4+ T cells are two important targets of human immunodeficiency virus (HIV) infection. Different strains of HIV-1 vary markedly in their abilities to infect cells belonging to the M/M lineage. Macrophagetropic (M-tropic) HIV-1 strains replicate well in primary lymphocytes as well as in primary macrophages; however, they generally infect T-cell lines poorly, if at all. Although promonocytic cell lines such as U937 have been used as in vitro models of HIV-1 infection of M/M, these cell lines are susceptible to certain T-cell-tropic (T-tropic) HIV-1 strai

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Matsumura, Yuko, Yasuhiko Ito, Yoshihiro Mezawa, et al. "Stromal fibroblasts induce metastatic tumor cell clusters via epithelial–mesenchymal plasticity." Life Science Alliance 2, no. 4 (2019): e201900425. http://dx.doi.org/10.26508/lsa.201900425.

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Emerging evidence supports the hypothesis that multicellular tumor clusters invade and seed metastasis. However, whether tumor-associated stroma induces epithelial–mesenchymal plasticity in tumor cell clusters, to promote invasion and metastasis, remains unknown. We demonstrate herein that carcinoma-associated fibroblasts (CAFs) frequently present in tumor stroma drive the formation of tumor cell clusters composed of two distinct cancer cell populations, one in a highly epithelial (E-cadherinhiZEB1lo/neg: Ehi) state and another in a hybrid epithelial/mesenchymal (E-cadherinloZEB1hi: E/M) state

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Takami, Akiyoshi, Shigeru Shimadoi, Yukio Kondo, Hirokazu Okumura, and Shinji Nakao. "Monocyte Colony-Stimulating Factor Enhances Rituximab-Dependent Cellular Cytotoxicity by Monocytes." Blood 108, no. 11 (2006): 2533. http://dx.doi.org/10.1182/blood.v108.11.2533.2533.

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Abstract [Introduction] Recent data suggest that monocytes are the dominant effector cells during immunotherapy using the anti-CD20 monoclonal antibody rituximab, depleting B cells through FcγR-dependent pathways. Because monocyte colony-stimulating factor (M-CSF) enhances the antibody-dependent cellular cytotoxicity (ADCC) of monocytes, the clinical efficacy of rituximab might be improved by the addition of M-CSF. We have studied the effect of M-CSF in the enhancement of rituximab-mediated ADCC against B-cell lymphoma. [Methods] Monocytes were isolated by negative selection of PBMCs from heal

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Johnston, J. B., G. Wang, J. W. Barrett, et al. "Myxoma Virus M-T5 Protects Infected Cells from the Stress of Cell Cycle Arrest through Its Interaction with Host Cell Cullin-1." Journal of Virology 79, no. 16 (2005): 10750–63. http://dx.doi.org/10.1128/jvi.79.16.10750-10763.2005.

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ABSTRACT The myxoma virus (MV) M-T5 gene encodes an ankyrin repeat protein that is important for virus replication in cells from several species. Insight was gained into the molecular mechanisms underlying the role of M-T5 as a host range determinant when the cell cycle regulatory protein cullin-1 (cul-1) was identified as a cellular binding partner of M-T5 and found to colocalize with the protein in both nuclear and cytosolic compartments. Consistent with this interaction, infection with wild-type MV (vMyxlac) or a deletion mutant lacking M-T5 (vMyxT5KO) differentially altered cell cycle prog

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Vorkas, Charles Kyriakos, Olivier Levy, Miroslav Skular, Kelin Li, Jeffrey Aubé, and Michael S. Glickman. "Efficient 5-OP-RU-Induced Enrichment of Mucosa-Associated Invariant T Cells in the Murine Lung Does Not Enhance Control of Aerosol Mycobacterium tuberculosis Infection." Infection and Immunity 89, no. 1 (2020): e00524-20. http://dx.doi.org/10.1128/iai.00524-20.

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ABSTRACTMucosa-associated invariant T (MAIT) cells are an innate-like T cell subset in mammals that recognize microbial vitamin B metabolites presented by the evolutionarily conserved major histocompatibility complex class I (MHC I)-related molecule, MR1. Emerging data suggest that MAIT cells may be an attractive target for vaccine-induced protection against bacterial infections because of their rapid cytotoxic responses at mucosal services to a widely conserved bacterial ligand. In this study, we tested whether a MAIT cell priming strategy could protect against aerosol Mycobacterium tuberculo

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Kim, Young-June, Adam T. Aisen, and Hal E. Broxmeyer. "Myeloid-Derived Suppressor Cell Regulation of PD-1 Expression on, and Longevity of, Human CD8+ T Cells." Blood 112, no. 11 (2008): 2562. http://dx.doi.org/10.1182/blood.v112.11.2562.2562.

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Abstract Persistent viral infections and tumor burdens are associated with limited longevity of functional CD8+ T cells. Longevity of effector or memory CD8+ T cells may depend on a balance of costimulatory and coinhibitory signals. 4–1BB (CD137), induced as a costimulatory receptor, is known to promote longevity of anti-virus and tumor CD8+ T cells, whereas ‘exhausted’ CD8+ T cells conversely induce coinhibitory receptors such as programmed death-1(PD-1) in order to limit long term CD8+ T cell survival. We hypothesized that myeloid-derived suppressor cells (MDSC) often recruited to the tumor

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Okumura, Daiki, Mari Hagino, Akane Yamagishi, et al. "Inhibitors of the VEGF Receptor Suppress HeLa S3 Cell Proliferation via Misalignment of Chromosomes and Rotation of the Mitotic Spindle, Causing a Delay in M-Phase Progression." International Journal of Molecular Sciences 19, no. 12 (2018): 4014. http://dx.doi.org/10.3390/ijms19124014.

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Cell division is the process by which replicated chromosomes are separated into two daughter cells. Although regulation of M phase has been extensively investigated, not all regulating factors have been identified. Over the course of our research, small molecules were screened to identify those that regulate M phase. In the present study, the vascular endothelial growth factor receptor (VEGFR) inhibitors A83-01, SU4312, and Ki8751 were examined to determine their effects on M phase. Treatment of HeLa S3 cells with these inhibitors suppressed cell proliferation in a concentration-dependent mann

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