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1

Degani, Ofir, Paz Becher, and Asaf Gordani. "Pathogenic Interactions between Macrophomina phaseolina and Magnaporthiopsis maydis in Mutually Infected Cotton Sprouts." Agriculture 12, no. 2 (2022): 255. http://dx.doi.org/10.3390/agriculture12020255.

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The soil fungus Macrophomina phaseolina, the charcoal rot disease agent, poses a major threat to cotton fields. In Israel, highly infected areas are also inhabited by the maize pathogen Magnaporthiopsis maydis. This study reveals the relationships between the two pathogens and their impact on cotton sprouts. Infecting the soil 14 days before sowing (DBS) with each pathogen or with M. phaseolina before M. maydis caused a strong inhibition (up to 50–65%) of the sprouts’ development and survival, accompanied by each pathogen’s high DNA levels in the plants. However, combined or sequence infection
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2

Dor and Degani. "Uncovering the Host Range for Maize Pathogen Magnaporthiopsis maydis." Plants 8, no. 8 (2019): 259. http://dx.doi.org/10.3390/plants8080259.

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The fungus Magnaporthiopsis maydis is a soil-borne, seed-borne vascular wilt pathogen that causes severe damage to sensitive Zea mays L. (maize) hybrids throughout Egypt, Israel, India, Spain, and other countries. It can undergo virulence variations and survive as spores, sclerotia, or mycelia on plant residues. Maize, Lupinus termis L. (lupine) and Gossypium hirsutum L. (cotton) are the only known hosts of M. maydis. Identification of new plant hosts that can assist in the survival of the pathogen is an essential step in restricting disease outbreak and spread. Here, by field survey and growt
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3

Fadayivata, Safoora, Gholamhossein Moravvej, and Javad Karimi. "Pathogenicity of the fungus Lecanicillium longisporum against Sipha maydis and Metopolophium dirhodum in laboratory conditions." Journal of Plant Protection Research 54, no. 1 (2014): 67–73. http://dx.doi.org/10.2478/jppr-2014-0010.

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Abstract This study aimed to evaluate the susceptibility of two cereal aphids, Sipha maydis (Passerini) and Metopolophium dirhodum (Walker), to the entomopathogenic fungus, Lecanicillium longisporum (Zimm.) Zare and Gams strain LRC 190, under controlled conditions. The conidial suspension of the fungus was administered using a sprayer on the whole plant over apterous adult aphids. The results indicated that both aphid species were susceptible to L. longisporum and that aphid populations were significantly reduced, compared to the control. Nine days after treatment, the LC50 value of the fungus
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4

Degani, Ofir, and Yuval Goldblat. "Potential Role of Laccases in the Relationship of the Maize Late Wilt Causal Agent, Magnaporthiopsis maydis, and Its Host." Journal of Fungi 6, no. 2 (2020): 63. http://dx.doi.org/10.3390/jof6020063.

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Late wilt is a vascular disease of maize (Zea mays L.) caused by the soil-borne and seed-borne fungus Magnaporthiopsis maydis. The pathogen penetrates the roots of maize plants at the seedling stage, grows into the xylem vessels, and gradually spreads upwards. From the flowering stage to the kernel ripening, the fungal hyphae and secreted materials block the water supply in susceptible maize cultivars, leading to rapid dehydration and death. Laccase is an enzyme secreted by fungus for diverse purposes. The M. maydis laccase gene was identified in our laboratory, but under what conditions it is
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5

Degani, Ofir, Soliman Khatib, Paz Becher, Asaf Gordani та Raviv Harris. "Trichoderma asperellum Secreted 6-Pentyl-α-Pyrone to Control Magnaporthiopsis maydis, the Maize Late Wilt Disease Agent". Biology 10, № 9 (2021): 897. http://dx.doi.org/10.3390/biology10090897.

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Late wilt disease (LWD) is a destructive vascular disease of maize (Zea mays L.) caused by the fungus Magnaporthiopsis maydis. Restricting the disease, which is a significant threat to commercial production in Israel, Egypt, Spain, India, and other countries, is an urgent need. In the past three years, we scanned nine Trichoderma spp. isolates as biological control candidates against M. maydis. Three of these isolates showed promising results. In vitro assays, seedlings pathogenicity trials, and field experiments all support the bio-control potential of these isolates (or their secretions). He
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6

Degani, Ofir, Shlomit Dor, Daniel Movshovitz, and Onn Rabinovitz. "Methods for Studying Magnaporthiopsis maydis, the Maize Late Wilt Causal Agent." Agronomy 9, no. 4 (2019): 181. http://dx.doi.org/10.3390/agronomy9040181.

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Late wilt, a destructive vascular disease of maize caused by the fungus Magnaporthiopsis maydis, is characterized by relatively fast wilting of maize plants closely before the physiological maturity stage. Previously, traditional microbiology-based methods have been used to isolate the pathogen and to characterize its traits. More recently, several molecular methods have been developed, enabling accurate and sensitive examination of the pathogen spread within the host. Here, we review the methods developed in the past 10 years in Israel, which include new or modified microbial and molecular te
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7

Casa, Ricardo T., Erlei M. Reis, and Laércio Zambolim. "Dispersão vertical e horizontal de conídios de Stenocarpella macrospora e Stenocarpella maydis." Fitopatologia Brasileira 29, no. 2 (2004): 141–47. http://dx.doi.org/10.1590/s0100-41582004000200004.

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Em experimentos conduzidos no campo, quantificaram-se a dispersão vertical e horizontal dos conídios de Stenocarpella maydis e S. macrospora liberados de palha de milho (Zea mays) naturalmente infetada. Verificou-se que 93% do número total de conídios de S. maydis e 88% de S. macrospora foram capturados durante o dia, diferindo estatisticamente do período noturno. O número de conídios de S. maydis e S. macrospora capturados no ar foi inversamente proporcional à distância da posição vertical e horizontal das armadilhas da fonte de inóculo. Os conídios foram capturados até uma altura de 2 m acim
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8

Veloso, Ronice Alves, Talita Pereira de Souza Ferreira, Daniel Debona, et al. "Enzymatic Activity in Essential Oil-Treated and Pathogen-Inoculated Corn Plants." Journal of Agricultural Science 10, no. 10 (2018): 171. http://dx.doi.org/10.5539/jas.v10n10p171.

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Bipolaris maydis and Exserohilum turcicum are important fungal pathogens that cause leaf blight in corn whose control have been difficult. Essential oils are a promising and environmentally friendly alternative for disease management, but the mechanisms of action remain poorly studied. Here, we aimed to assess the effect of B. maydis and E. turcicum as well as the essential oil of Morinda citrifolia in the activity of plant defense enzymes in corn plants. Experiments were carried out in a completely randomized design with three replications and six treatments as they follow: (T1): corn plants
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9

Puterka, Gary J., Robert W. Hammon, Melissa Franklin, et al. "Distribution of a New Invasive Species, Sipha maydis (Heteroptera: Aphididae), on Cereals and Wild Grasses in the Southern Plains and Rocky Mountain States." Journal of Economic Entomology 112, no. 4 (2019): 1713–21. http://dx.doi.org/10.1093/jee/toz068.

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Abstract Sipha maydis Passerini (Heteroptera: Aphididae) is a cereal pest with an extensive geographical range that includes countries in Europe, Asia, Africa, and South America. Reports of S. maydis in the United States have been infrequent since it was first detected in California, 2007. Two studies, focused (NW CO) and multistate (OK, TX, NM, CO, UT, WY), were conducted to determine the distribution and host range of S. maydis in the Rocky Mountain and Southern Plains states over a 3-yr period, 2015–2017. In 2015, focused sampling in NW Colorado found S. maydis at 59% of the 37 sites, prima
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10

Degani, Ofir, Danielle Regev, and Shlomit Dor. "The Microflora of Maize Grains as a Biological Barrier against the Late Wilt Causal Agent, Magnaporthiopsis maydis." Agronomy 11, no. 5 (2021): 965. http://dx.doi.org/10.3390/agronomy11050965.

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The maize pathogen Magnaporthiopsis maydis causes severe damage to commercial fields in the late growth stages. This late wilt disease has spread since its discovery (the 1980s) and is now common in most corn-growing areas in Israel. In some fields and sensitive plant species, the disease can affect 100% of the plants. The M. maydis pathogen has a hidden endophytic lifecycle (developed inside the plants with no visible symptoms) in resistant corn plants and secondary hosts, such as green foxtail and cotton. As such, it may also be opportunist and attack the host in exceptional cases when condi
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11

Xu, Yuze, and David T. Stuart. "Moesziomyces antarcticus MMF1 Has a Role in the Secretion of Mannosylerythritol Lipids." Microorganisms 13, no. 7 (2025): 1463. https://doi.org/10.3390/microorganisms13071463.

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Mannosyl erythritol lipids (MELs) are glycolipid biosurfactants produced by Ustilaginomycete yeasts. The MEL biosynthetic pathway has been characterized in Ustilago maydis where a putative transporter encoded by MMF1 is required for the secretion of the glycolipid surfactant to the extracellular space. The anamorphic yeast Moesziomyces antarcticus is a prolific producer of MELs, but the mechanism of MEL secretion is less well characterized than in U. maydis. Homologous recombination was employed to generate a disruption of the MMF1 gene in M. antarcticus JCM10317. This mutation did not prevent
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12

Campos, Maria, Mariana Patanita, Catarina Campos, et al. "Detection and Quantification of Fusarium spp. (F. oxysporum, F. verticillioides, F. graminearum) and Magnaporthiopsis maydis in Maize Using Real-Time PCR Targeting the ITS Region." Agronomy 9, no. 2 (2019): 45. http://dx.doi.org/10.3390/agronomy9020045.

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Fusarium spp. and Magnaporthiopsis maydis are soil-inhabiting fungi and respectively the causal agents of fusarium ear rot and late wilt, two important diseases that can affect maize, one of the most important cereal crops worldwide. Here, we present two sensitive real-time PCR TaqMan MGB (Minor Groove Binder) assays that detect and discriminate several Fusarium spp. (F. oxysporum, F. verticillioides, and F. graminearum) from M. maydis. The method is based on selective real-time qPCR amplification of the internal transcribed spacer (ITS) region and allows the quantification of the fungi. The a
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13

Patanita, Mariana, Maria Doroteia Campos, Maria do Rosário Félix, Mário Carvalho, and Isabel Brito. "Effect of Tillage System and Cover Crop on Maize Mycorrhization and Presence of Magnaporthiopsis maydis." Biology 9, no. 3 (2020): 46. http://dx.doi.org/10.3390/biology9030046.

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The sustainability of agriculture requires the adoption of agricultural soil conservation practices with positive impacts on soil quality, which can promote beneficial soil microbiota like arbuscular mycorrhizal fungi (AMF) and its diversity. This study aims to assess the influence of the presence of intact extraradical mycelium as a preferential source of inoculum of the native AMF in order to guarantee a better colonization as well as its possible bioprotective effect against Magnaporthiopsis maydis. In order to vary the available extraradical mycelium, two experiments, with and without cove
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14

Reis, Erlei M., and Justino L. Mario. "Quantificação do inóculo de Diplodia macrospora e de D. maydis em restos culturais, no ar, e sua relação com a infecção em grãos de milho." Fitopatologia Brasileira 28, no. 2 (2003): 143–47. http://dx.doi.org/10.1590/s0100-41582003000200004.

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A quantificação do inóculo de Diplodia macrospora e de D. maydis nos restos culturais do milho (Zea mays)é fundamental para demonstrar a importância desta fonte de inóculo, bem como servir de base para o desenvolvimento de estratégias alternativas de controle. Em experimentos conduzidos no campo, nas safras de verão de 1995/96 e 1996/97, quantificaram-se a quantidade de restos culturais do milho sobre o solo após a semeadura, a densidade de conídiosnos resíduos e o inóculo disseminado pelo ar, bem como a incidência de cada espécie de Diplodia nos grãos de milho colhidos. A quantidade de conídi
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15

Paterniani, Maria Elisa Ayres Guidetti Zagatto, Eduardo Sawazaki, Christina Dudienas, Aildson Pereira Duarte, and Paulo Boller Gallo. "Diallel crosses among maize lines with emphasis on resistance to foliar diseases." Genetics and Molecular Biology 23, no. 2 (2000): 381–85. http://dx.doi.org/10.1590/s1415-47572000000200024.

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Ten elite maize (Zea mays L.) lines were crossed in a complete diallel scheme and the single-cross hybrids obtained were assessed at four experimental stations of the Agronomic Institute of Campinas, in São Paulo State, Brazil. The experiments were set up in a randomized complete block design with three replications, including four commercial checks. The experimental plots consisted of two 5-m rows spaced at 0.9 m, with a total of 50 plants. The traits assessed included: days to mid-tassel pollen shed (DPS), plant height (PH), ear height (EH), grain yield, corrected for a 50-plant stand and 14
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16

de la Torre, Antonio, Sónia Castanheira, and José Pérez-Martín. "Incompatibility between proliferation and plant invasion is mediated by a regulator of appressorium formation in the corn smut fungusUstilago maydis." Proceedings of the National Academy of Sciences 117, no. 48 (2020): 30599–609. http://dx.doi.org/10.1073/pnas.2006909117.

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Plant pathogenic fungi often developed specialized infection structures to breach the outer surface of a host plant. These structures, called appressoria, lead the invasion of the plant by the fungal hyphae. Studies in different phytopathogenic fungi showed that appressorium formation seems to be subordinated to the cell cycle. This subordination ensures the loading in the invading hypha of the correct genetic information to proceed with plant infection. However, how the cell cycle transmits its condition to the genetic program controlling appressorium formation and promoting the plant’s invas
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17

Steinberg, G., R. Wedlich-Soldner, M. Brill, and I. Schulz. "Microtubules in the fungal pathogen Ustilago maydis are highly dynamic and determine cell polarity." Journal of Cell Science 114, no. 3 (2001): 609–22. http://dx.doi.org/10.1242/jcs.114.3.609.

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Many fungal pathogens undergo a yeast-hyphal transition during their pathogenic development that requires rearrangement of the cytoskeleton, followed by directed membrane traffic towards the growth region. The role of microtubules and their dynamic behavior during this process is not well understood. Here we set out to elucidate the organization, cellular role and in vivo dynamics of microtubules in the dimorphic phytopathogen Ustilago maydis. Hyphae and unbudded yeast-like cells of U. maydis contain bundles of spindle pole body-independent microtubules. At the onset of bud formation two spher
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18

Degani, Ofir, Shlomit Dor, Dekel Abraham, and Roni Cohen. "Interactions between Magnaporthiopsis maydis and Macrophomina phaseolina, the Causes of Wilt Diseases in Maize and Cotton." Microorganisms 8, no. 2 (2020): 249. http://dx.doi.org/10.3390/microorganisms8020249.

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Fungal pathogens are a significant threat to crops worldwide. The soil fungus, Magnaporthiopsis maydis, severely affects sensitive maize hybrids by causing the rapid wilting of plants at the maturity stage. Similarly, the soil fungus, Macrophomina phaseolina, develops in a variety of host plants, which leads to rot and plant mortality. The presence of both pathogens together in diseased cotton plants in Israel suggests possible interactions between them. Here, these relationships were tested in a series of experiments accompanied by real-time PCR tracking in maize and cotton. Despite the fact
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19

Abdel-Kader, M. M., M. S. A. Khalil, and N. S. El-Mougy. "Efficacy of fungicide alternatives against late wilt disease of maize and their influence on plant morphogenesis and yield characters." Hellenic Plant Protection Journal 15, no. 2 (2022): 57–71. http://dx.doi.org/10.2478/hppj-2022-0007.

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Summary Efficiency of various organic acids, organic salts, essential oils, algae (an extract of Chlorella vulgaris and a commercial product), and bioagents against Magnaporthiopsis maydis, causing maize late wilt disease, was evaluated in laboratory and field conditions. For the in vitro tests, isolated M. maydis field strains from Egypt were used. Additionally, in field experiments different application methods were tested for their efficacy throughout two successive growing seasons. Results showed maximum growth inhibition of M. maydis at different concentrations of salicylic acid, ascorbic
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20

Degani, Ofir, Danielle Regev, Shlomit Dor, and Onn Rabinovitz. "Soil Bioassay for Detecting Magnaporthiopsis maydis Infestation Using a Hyper Susceptible Maize Hybrid." Journal of Fungi 6, no. 3 (2020): 107. http://dx.doi.org/10.3390/jof6030107.

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Magnaporthiopsis maydis is the causal agent of severe maize late wilt disease. Disease outbreak occurs at the maize flowering and fruit development stage, leading to the plugging of the plant’s water vascular system, resulting in dehydration and collapse of the infected host plant. The pathogen is borne by alternative hosts, infected seeds, soil, and plant residues and gradually spreads to new areas and new countries. However, no soil assay is available today that can detect M. maydis infestation and study its prevalence. We recently developed a molecular quantitative Real-Time PCR (qPCR) meth
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21

Degani, Ofir, та Asaf Gordani. "New Antifungal Compound, 6-Pentyl-α-Pyrone, against the Maize Late Wilt Pathogen, Magnaporthiopsis maydis". Agronomy 12, № 10 (2022): 2339. http://dx.doi.org/10.3390/agronomy12102339.

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6-pentyl-α-pyrone (6-PP) is a powerful Magnaporthiopsis maydis antifungal compound, recently discovered when the potent growth medium of Trichoderma asperellum was analyzed. Despite its high potential in plate assay, it was not inspected for plant treatment prevention. Late wilt disease, caused by the fungus M. maydis, threatens commercial maize production in high-risk areas. Thus, the search for control options against the pathogen is one of the top priorities in Israel, Egypt, and other countries. Disease-resistant maize genotypes can reduce the damages. Yet, aggressive variants of the fungu
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22

DENTI, EIDI ALFREDO, and ERLEI MELO REIS. "Efeito da rotação de culturas, da monocultura e da densidade de plantas na incidência das podridões da base do colmo e no rendimento de grãos do milho." Fitopatologia Brasileira 26, no. 3 (2001): 635–39. http://dx.doi.org/10.1590/s0100-41582001000300009.

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Quantificou-se neste trabalho o efeito da rotação de culturas, da monocultura e da densidade de plantas na incidência de doenças e no rendimento de grãos, associados às podridões da base do colmo (PBC) do milho (Zea mays), em experimentos de campo conduzidos na área experimental da Universidade de Passo Fundo (RS) durante a safra 1998/99. Utilizou-se o genótipo Pioneer 3071, semeado em parcelas de quatro linhas de 5 m distribuídas em blocos ao acaso e cinco tratamentos (30, 40, 50 ,60, e 70 mil plantas ha-1) com quatro repetições. As avaliações da incidência das PBC foram realizadas a cada set
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23

Mielnichuk, Natalia, and José Pérez-Martín. "14-3-3 regulates the G2/M transition in the basidiomycete Ustilago maydis." Fungal Genetics and Biology 45, no. 8 (2008): 1206–15. http://dx.doi.org/10.1016/j.fgb.2008.05.010.

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Bison, Odair, Magno Antonio Patto Ramalho, and Francislei Vitti Raposo. "Potencial de híbridos simples de milho para extração de linhagens." Ciência e Agrotecnologia 27, no. 2 (2003): 348–55. http://dx.doi.org/10.1590/s1413-70542003000200014.

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A utilização de híbridos simples comerciais de milho é uma das opções de populações para a extração de linhagens, porque são adaptados e provavelmente concentram alta freqüência de alelos favoráveis já fixados. Mesmo nos locos que estão segregando, a freqüência de alelos favoráveis é 0,5. Assim, a identificação de populações promissoras, derivadas de híbridos simples superiores, é uma boa estratégia para aumentar a eficiência dos programas de melhoramento. As populações derivadas dos híbridos simples comerciais AG9012 e C333 foram avaliadas com o objetivo de verificar o potencial dessas para e
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25

Silva, Herberte Pereira da, and José Roberto Moro. "Diallel analysis of maize resistance to Phaeosphaeria maydis." Scientia Agricola 61, no. 1 (2004): 36–42. http://dx.doi.org/10.1590/s0103-90162004000100007.

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In breeding programs directed towards genetic resistance against diseases, the estimation of genetic parameters that control resistance allows the introduction of resistance into suscetible germplasm to be clearly focused. The purposes of this study were to estimate heterosis effects, and the general (GCA) and specific (SCA) combining abilities by using two rating methods for resistance to Phaeosphaeria maydis in a diallel analysis of 36 F1 maize hybrids and their nine inbred lines. Trials were conducted in three environments. Disease severity was evaluated in the whole plant (PI) and in the l
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SHAH, JA, U. RAMZAN, S. NASEER, et al. "CHEMICAL CONTROL OF SOUTHERN LEAF BLIGHT OF MAIZE CAUSED BY HELMINTHOSPORIUM MAYDIS." Biological and Clinical Sciences Research Journal 2023, no. 1 (2023): 225. http://dx.doi.org/10.54112/bcsrj.v2023i1.225.

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Southern Leaf Blight of maize (SLBM) is a severe disease affecting maize crop in Pakistan. This disease causes considerable losses in maize crop every year. To avoid this loss, a study was conducted to check the efficacy of four fungicides and one control, tested against maize variety Malka-2016. The experiment was conducted at Plant Pathology AARI Research area, Faisalabad, during 2021-22. The result showed that Propiconazole (Tilt 25% WP) was found to be the most effective fungicide to control the leaf Blight of maize followed by Dithane M-45 (Mancozeb 80%WP) and Kavach (Chlorothalonil 75%WP
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Degani, Ofir, and Shlomit Dor. "Trichoderma Biological Control to Protect Sensitive Maize Hybrids against Late Wilt Disease in the Field." Journal of Fungi 7, no. 4 (2021): 315. http://dx.doi.org/10.3390/jof7040315.

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Late wilt, a disease severely affecting maize fields throughout Israel, is characterized by the relatively rapid wilting of maize plants from the tasseling stage to maturity. The disease is caused by the fungus Magnaporthiopsis maydis, a soil and seed-borne pathogen. The pathogen is controlled traditionally through the use of maize cultivars having reduced sensitivity to the disease. Nevertheless, such cultivars may lose their immunity after several years of intensive growth due to the presence of high virulent isolates of M. maydis. Alternative effective and economical chemical treatment to t
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28

Degani, Ofir, Onn Rabinovitz, Paz Becher, Asaf Gordani, and Assaf Chen. "Trichoderma longibrachiatum and Trichoderma asperellum Confer Growth Promotion and Protection against Late Wilt Disease in the Field." Journal of Fungi 7, no. 6 (2021): 444. http://dx.doi.org/10.3390/jof7060444.

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Late wilt disease (LWD) of maize, caused by Magnaporthiopsis maydis, is considered a major threat to commercial fields in Israel, Egypt, Spain, and India. Today’s control methods include chemical and agronomical intervention but rely almost solely on resistant maize cultivars. In recent years, LWD research focused on eco-friendly biological approaches to restrain the pathogen. The current study conducted during two growing seasons explores the potential of three Trichoderma species as bioprotective treatments against LWD. These species excelled in preliminary assays performed previously under
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29

Tanaka, K., J. Davey, Y. Imai, and M. Yamamoto. "Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor." Molecular and Cellular Biology 13, no. 1 (1993): 80–88. http://dx.doi.org/10.1128/mcb.13.1.80-88.1993.

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A defect in the map3 gene of the fission yeast Schizosaccharomyces pombe causes h+ mating-type-specific sterility. This gene was cloned by complementation. Nucleotide sequence analysis showed that it has a coding capacity of 365 amino acids. The deduced map3 gene product is a putative seven-transmembrane protein and has 20.0% amino acid identity with the a-factor receptor of Saccharomyces cerevisiae, encoded by STE3. It is also homologous with the Ustilago maydis mating pheromone receptors. The map3 gene is expressed in h+ cells but not in h- cells, and the transcripts are induced in response
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Tanaka, K., J. Davey, Y. Imai, and M. Yamamoto. "Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor." Molecular and Cellular Biology 13, no. 1 (1993): 80–88. http://dx.doi.org/10.1128/mcb.13.1.80.

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A defect in the map3 gene of the fission yeast Schizosaccharomyces pombe causes h+ mating-type-specific sterility. This gene was cloned by complementation. Nucleotide sequence analysis showed that it has a coding capacity of 365 amino acids. The deduced map3 gene product is a putative seven-transmembrane protein and has 20.0% amino acid identity with the a-factor receptor of Saccharomyces cerevisiae, encoded by STE3. It is also homologous with the Ustilago maydis mating pheromone receptors. The map3 gene is expressed in h+ cells but not in h- cells, and the transcripts are induced in response
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31

Shukla, R. S., P. K. Agrawal, R. S. Thakur, and A. Husain. "Drechslerol-C, a phytotoxin produced by Drechslera maydis, the causal organism of leaf blight of Costus speciosus." Plant Science 66, no. 1 (1990): 43–49. http://dx.doi.org/10.1016/0168-9452(90)90167-m.

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32

Sgarlata, Cecilia, and José Pérez-Martín. "The cdc25 phosphatase is essential for the G2/M phase transition in the basidiomycete yeast Ustilago maydis." Molecular Microbiology 58, no. 5 (2005): 1482–96. http://dx.doi.org/10.1111/j.1365-2958.2005.04925.x.

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33

Peery, T., T. Shabat-Brand, R. Steinlauf, Y. Koltin, and J. Bruenn. "Virus-encoded toxin of Ustilago maydis: two polypeptides are essential for activity." Molecular and Cellular Biology 7, no. 1 (1987): 470–77. http://dx.doi.org/10.1128/mcb.7.1.470-477.1987.

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Cells of Ustilago maydis containing double-stranded RNA viruses secrete a virus-encoded toxin to which other cells of the same species and related species are sensitive. Mutants affected in the expression of the KP6 toxin were characterized, and all were viral mutants. A temperature-sensitive nonkiller mutant indicated that the toxin consists of two polypeptides, 12.5K and 10K, that are essential for the toxic activity. The temperature-sensitive nonkiller mutant was affected in the expression of the 10K polypeptide, and its toxic activity was restored by the addition of the 10K polypeptide to
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34

Peery, T., T. Shabat-Brand, R. Steinlauf, Y. Koltin, and J. Bruenn. "Virus-encoded toxin of Ustilago maydis: two polypeptides are essential for activity." Molecular and Cellular Biology 7, no. 1 (1987): 470–77. http://dx.doi.org/10.1128/mcb.7.1.470.

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Cells of Ustilago maydis containing double-stranded RNA viruses secrete a virus-encoded toxin to which other cells of the same species and related species are sensitive. Mutants affected in the expression of the KP6 toxin were characterized, and all were viral mutants. A temperature-sensitive nonkiller mutant indicated that the toxin consists of two polypeptides, 12.5K and 10K, that are essential for the toxic activity. The temperature-sensitive nonkiller mutant was affected in the expression of the 10K polypeptide, and its toxic activity was restored by the addition of the 10K polypeptide to
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35

Degani, Ofir. "Control Strategies to Cope with Late Wilt of Maize." Pathogens 11, no. 1 (2021): 13. http://dx.doi.org/10.3390/pathogens11010013.

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Control of maize late wilt disease (LWD) has been at the forefront of research efforts since the discovery of the disease in the 1960s. The disease has become a major economic restraint in highly affected areas such as Egypt and Israel, and is of constant concern in other counties. LWD causes dehydration and collapsing at a late stage of maize cultivation, starting from the male flowering phase. The disease causal agent, Magnaporthiopsis maydis, is a seed- and soil-borne phytoparasitic fungus, penetrating the roots at sprouting, colonizing the vascular system without external symptoms, and spr
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36

Degani, Ofir. "A Review: Late Wilt of Maize—The Pathogen, the Disease, Current Status, and Future Perspective." Journal of Fungi 7, no. 11 (2021): 989. http://dx.doi.org/10.3390/jof7110989.

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Late wilt (LWD) is a vascular wilt disease that outbursts late in maize development, usually during or after flowering. The disease causal agent, the soil and seed-borne fungi, Magnaporthiopsis maydis, causes significant economic losses in Egypt, Israel, Spain, Portugal, and India. Since its discovery in the early 1960s in Egypt, the knowledge base of the disease was significantly expanded. This includes basic information on the pathogen and its mode of action, disease symptoms and damages, methods to study and monitor the pathogen, and above all, control strategies to restrain M. maydis and r
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37

Prakriti, Sharma, Nepal Sallu, Paudel Sareeta, Bhandari Srijana, K.C Sujata, and Man Shrestha Sundar. "Screening of Maize Genotypes against Southern Leaf Blight (Bipolaris Maydis) during summer in Rampur, Chitwan." International Journal of Environmental & Agriculture Research 4, no. 2 (2018): 54–62. https://doi.org/10.5281/zenodo.1188096.

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<em>Screening of 20 maize genotypes against Southern Leaf Blight of maize (Bipolaris maydis (Nisik) Shoemaker) was carried out in RCBD in National Maize Research Program, Rampur, Nepal during June to September, 2015. The area of research field was 315 m<sup>2</sup>. Each genotype had two rows per plot of 7.5m<sup>2</sup> with two replication. Disease incidence was taken for three times at 43, 53 and 63 DAS. Disease scoring was done as percentage of leaf area infected on individual plant at 7 days interval starting from 58 days after sowing for 5 times and disease severity and mean AUDPC was ca
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38

Chen, Ming, Wen-Gui Duan, Gui-Shan Lin, Zhong-Tian Fan, and Xiu Wang. "Synthesis, Antifungal Activity, and 3D-QSAR Study of Novel Nopol-Derived 1,3,4-Thiadiazole-Thiourea Compounds." Molecules 26, no. 6 (2021): 1708. http://dx.doi.org/10.3390/molecules26061708.

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A series of novel nopol derivatives bearing the 1,3,4-thiadiazole-thiourea moiety were designed and synthesized by multi-step reactions in search of potent natural product-based antifungal agents. Their structures were confirmed by FT-IR, NMR, ESI-MS, and elemental analysis. Antifungal activity of the target compounds was preliminarily evaluated by in vitro methods against Fusarium oxysporum f. sp. cucumerinum, Cercospora arachidicola, Physalospora piricola, Alternaria solani, Gibberella zeae, Rhizoeotnia solani, Bipolaris maydis, and Colleterichum orbicalare at 50 µg/mL. All the target compou
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Degani, Ofir, Ran Yifa, Asaf Gordani, Paz Becher, and Assaf Chen. "Cultivars Resistance Assay for Maize Late Wilt Disease." Biology 11, no. 12 (2022): 1854. http://dx.doi.org/10.3390/biology11121854.

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Magnaporthiopsis maydis late wilt disease (LWD) in corn is considered to be the most severe in Israel and Egypt and poses a significant threat in other countries. Research efforts extending over a period of five decades led to the development of chemical, biological, agrotechnical, physical (solar disinfection) and other means for controlling late wilt disease. Today, some applications can reduce damage even in severe cases. However, cultivating disease-resistant maize varieties is the primary means for reducing the disease’s impact. The current work uses a rapid (six days) laboratory seedling
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Tian, Yao, Jinchao Shi, Xiaoqian Deng, et al. "Design, Synthesis, and Antifungal Activity of Some Novel Phenylthiazole Derivatives Containing an Acylhydrazone Moiety." Molecules 28, no. 20 (2023): 7084. http://dx.doi.org/10.3390/molecules28207084.

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Crop fungal diseases pose a serious threat to global crop production and quality. Developing new and efficient fungicides is an important measure to control crop diseases. Phenylthiazole was found to be an excellent antifungal skeleton based on our previous study on the structural optimization and biological activity of the natural product thiasporine A. To find new fungicides, 45 phenylthiazole derivatives containing an acylhydrazone moiety were designed and synthesized by the principle of active substructure splicing. Forty-two of the forty-five compounds are novel, except for compounds E1,
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41

Del Prete, Sonia, Daniela Vullo, Cynthia Ghobril та ін. "Cloning, Purification, and Characterization of a β-Carbonic Anhydrase from Malassezia restricta, an Opportunistic Pathogen Involved in Dandruff and Seborrheic Dermatitis". International Journal of Molecular Sciences 20, № 10 (2019): 2447. http://dx.doi.org/10.3390/ijms20102447.

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The cloning, purification, and initial characterization of the β-carbonic anhydrase (CA, EC 4.2.1.1) from the genome of the opportunistic pathogen Malassezia restricta (MreCA), which a fungus involved in dandruff and seborrheic dermatitis (SD), is reported. MreCA is a protein consisting of 230 amino acid residues and shows high catalytic activity for the hydration of CO2 into bicarbonate and protons, with the following kinetic parameters: kcat of 1.06 × 106 s−1 and kcat/KM of 1.07 × 108 M−1 s−1. It is also sensitive to inhibition by the sulfonamide acetazolamide (KI of 50.7 nM). Phylogenetical
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42

Zhou, Hu. "Characterization and Inhibitory Effects of an Antifungal Protein from the Bacillus cereus Strain YN917." International Journal of Agriculture and Biology 25, no. 05 (2021): 1153–60. http://dx.doi.org/10.17957/ijab/15.1776.

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In this research, a type of antifungal protein derived from the Bacillus cereus strain YN917, was precipitated by ammonium sulfate after fermentation. We investigated the antifungal effects and antifungal mechanism of the protein as well as its stability under different stress conditions such as the variation of temperature, pH value, exposure to ultraviolet radiation and proteases. Furthermore, the in vitro needle inoculation method was carried out to explore the antagonistic effects of 40% isoprothiolane Wettable powder (WP), 20% tricyclazole WP and the YN917 antifungal protein against the r
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43

Onel, Kenan, Andrew Koff, Richard L. Bennett, Paul Unrau, and William K. Holloman. "The REC1 Gene of Ustilago maydis, Which Encodes a 3′ → 5′ Exonuclease, Couples DNA Repair and Completion of DNA Synthesis to a Mitotic Checkpoint." Genetics 143, no. 1 (1996): 165–74. http://dx.doi.org/10.1093/genetics/143.1.165.

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Abstract Mutation in the REC1 gene of Ustilago maydis results in extreme sensitivity to killing by ultraviolet light. The lethality of the rec1-1 mutant was found to be partially suppressed if irradiated cells were held artificially in G2-phase by addition of a microtubule inhibitor. This mutant was also found to be sensitive to killing when DNA synthesis was inhibited by external means through addition of hydroxyurea or by genetic control in a temperature-sensitive mutant strain defective in DNA synthesis. Flow cytometric analysis of exponentially growing cultures indicated that wild-type cel
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44

Gao, Weimin, Chang Hyun Khang, Sook-Young Park, Yong-Hwan Lee, and Seogchan Kang. "Evolution and Organization of a Highly Dynamic, Subtelomeric Helicase Gene Family in the Rice Blast Fungus Magnaporthe grisea." Genetics 162, no. 1 (2002): 103–12. http://dx.doi.org/10.1093/genetics/162.1.103.

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Abstract Sequence analysis of a 13-kb telomeric region in O-137, a rice pathogenic isolate of Magnaporthe grisea, uncovered a novel gene, designated TLH1 (telomere-linked helicase 1). The TLH1 gene is a member of a gene family, and the sequences flanking this gene family have also been amplified. Genetic mapping showed that most members of the TLH gene family are tightly linked to the telomeres. A physical mapping technique, termed RecA-mediated Achilles’ heel cleavage, and cloning and sequencing of two additional telomeres of O-137 associated with the TLH gene family confirmed that most membe
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45

Berbee, Mary L., Robert Bauer, and F. Oberwinkler. "The spindle pole body cycle, meiosis, and basidial cytology of the smut fungus Microbotryum violaceum." Canadian Journal of Botany 69, no. 8 (1991): 1795–803. http://dx.doi.org/10.1139/b91-228.

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Freeze-substituted basidia of the smut fungus Microbotryum violaceum (Ustilaginales, Basidiomycotina) were examined electron microscopically with particular attention to the meiotic spindle pole body cycle and cytoplasmic characters of phylogenetic significance. Prophase basidia contained a subapical cluster of vesicles and tubules. During prophase, the spindle pole body consisted of two globular elements connected by a middle piece. The spindle pole body had an electron-opaque layer near the nucleus, and each globular element was bisected by an electron-opaque disk. The meiosis I spindle exte
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Huang, Mei, Wen-Gui Duan, Gui-Shan Lin, and Bao-Yu Li. "Synthesis, Antifungal Activity, 3D-QSAR, and Molecular Docking Study of Novel Menthol-Derived 1,2,4-Triazole-thioether Compounds." Molecules 26, no. 22 (2021): 6948. http://dx.doi.org/10.3390/molecules26226948.

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A series of novel menthol derivatives containing 1,2,4-triazole-thioether moiety were designed, synthesized, characterized structurally, and evaluated biologically to explore more potent natural product-based antifungal agents. The bioassay results revealed that at 50 μg/mL, some of the target compounds exhibited good inhibitory activity against the tested fungi, especially against Physalospora piricola. Compounds 5b (R = o-CH3 Ph), 5i (R = o-Cl Ph), 5v (R = m,p-OCH3 Ph) and 5x (R = α-furyl) had inhibition rates of 93.3%, 79.4%, and 79.4%, respectively, against P. piricola, much better than th
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Williams, KC, BJ Blaney, RL Dodman, and CL Palmer. "Assessment for animal feed of maize kernels naturally-infected predominantly with Fusarium moniliforme and Diplodia maydis. I. Fungal isolations and changes in chemical composition." Australian Journal of Agricultural Research 43, no. 4 (1992): 773. http://dx.doi.org/10.1071/ar9920773.

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A study was made of the physical characteristics, fungal associations and the chemical composition of five batches of maize grain (designated A, B, J, M and P) damaged by ear-rot. All batches were the yellow hybrid, Dekalb XL81, except batch A which was a white variety, Dekalb DS456W. Batch A was separated by vibrating gravity grading into heavy (A-H) and light (A-L) fractions. For each batch, kernels were sorted visually into sound and mould-damaged samples to examine for any differences. The proportion of sound kernels in batches A, A-H, A-L, B, J, M and P was 874, 926, 260, 830, 854, 789 an
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Roberdi, Roberdi S., Hajrial S. Aswidinnoor, Asep S. Setiawan, Sutrisno S. Sutrisno, Marcia B. Pabendon, and Muhammad S. Azrai. "Keterpautan 23 Marka Mikrosatelit pada Kromosom 6 dan 7 dengan Karakter Ketahanan Populasi Jagung terhadap Penyakit Bulai (Peronosclerospora maydis)." Jurnal AgroBiogen 6, no. 1 (2016): 10. http://dx.doi.org/10.21082/jbio.v6n1.2010.p10-17.

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&lt;p&gt;Linkage of 23 Microsatellite Marker on Chromosome 6&lt;br /&gt;and 7 to Downy Mildew Resistance on Maize. Roberdi,&lt;br /&gt;Hajrial Aswidinnoor, Asep Setiawan, Sutrisno, Marcia B.&lt;br /&gt;Pabendon, and M. Azrai. Downy mildew caused by&lt;br /&gt;Peronosclerospora is one of most important maize disease&lt;br /&gt;in several countries, including Indonesia. Parental and&lt;br /&gt;progenies selection based on conventional breeding is time&lt;br /&gt;consuming and laborious. Development of molecular&lt;br /&gt;biology produces many DNA markers used for selection, one&lt;br /&gt;of th
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Garraway, M. O., D. S. Park, and J. D. Beltran. "Role of Light and Malate in the Decreased Sensitivity of cms-T Cytoplasm Maize Leaves to Bipolaris maydis Race T Toxin." Phytopathology® 88, no. 6 (1998): 556–62. http://dx.doi.org/10.1094/phyto.1998.88.6.556.

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Leaf segments from Texas male sterile (cms-T) cytoplasm maize isolines exposed to light (50 μM s-1 m-2) for 8 h or more before or after being infiltrated with the Bipolaris maydis race T toxin (T-toxin) leaked significantly less electrolytes when immersed in distilled water (DW) for 24 to 48 h than did dark-treated leaf segments. No comparable effect of light on toxin-induced electrolyte leakage was observed with normal (N) cytoplasm isolines. Toxin-treated cms-T leaf segments incubated in DW for three consecutive 12-h periods of alternating light and dark showed significantly greater electrol
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Barros, Alessandra Macedo, Talita Pereira de Souza Ferreira, Dalmarcia De Souza Carlos Mourão, Raimundo Wagner De Souza Aguiar, and Gil Rodrigues dos Santos. "Levantamento e uso de plantas medicinais do cerrado tocantinense para o controle alternativo de fitopatógenos." Journal of Biotechnology and Biodiversity 7, no. 3 (2019): 336–46. http://dx.doi.org/10.20873/jbb.uft.cemaf.v7n3.barros.

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A cultura do milho e feijão é destaque no Brasil e Tocantins. Entretanto, doenças tem influenciado na perda de produtividade dessas cultivares. Logo, o presente estudo objetivou, fazer um levantamento das plantas medicinais do cerrado tocantinense e avaliar o potencial fungitóxico de óleos essenciais destas plantas, com vistas ao controle alternativo de fitopatógenos em milho e feijão caupi. Foram registradas e selecionadas 20 espécies de plantas utilizadas na medicina popular no Município de Peixe, TO. O fungo isolado do milho foi o B. maydis e do feijão caupi o fungo R. solani, ambos cultiva
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