Academic literature on the topic 'M. morganii 16s rdna analysis'

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Journal articles on the topic "M. morganii 16s rdna analysis"

1

KIM, SHIN-HEE, HAEJUNG AN, KATHARINE G. FIELD, et al. "Detection of Morganella morganii, a Prolific Histamine Former, by the Polymerase Chain Reaction Assay with 16S rDNA–Targeted Primers." Journal of Food Protection 66, no. 8 (2003): 1385–92. http://dx.doi.org/10.4315/0362-028x-66.8.1385.

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A polymerase chain reaction (PCR) assay for the rapid and sensitive detection of the most prolific histamine former, Morganella morganii, was developed.16S rDNA targeted PCR primers were designed, and the primer specificity and sensitivity of the PCR assay were evaluated. The 16S rDNA sequence (1,503 bp) for M. morganii showed 95% identity to those for enteric bacteria, i.e., Enterobacter spp., Klebsiella spp., Citrobacter spp., Hafnia alvei, Proteus spp., and Providencia spp. The unique primers for M. morganii were designed on the basis of the variable regions in the 16S rDNA sequence. The pr
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Emborg, Jette, Paw Dalgaard, and Peter Ahrens. "Morganella psychrotolerans sp. nov., a histamine-producing bacterium isolated from various seafoods." International Journal of Systematic and Evolutionary Microbiology 56, no. 10 (2006): 2473–79. http://dx.doi.org/10.1099/ijs.0.64357-0.

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Mesophilic Morganella morganii (n=6) and psychrotolerant M. morganii-like isolates from various seafoods (n=13), as well as clinical M. morganii isolates (n=3), were characterized by using a polyphasic approach including multi-locus sequencing. Based on the phylogenetic analysis, the 22 strains were divided into two distinct groups comprising mesophilic and psychrotolerant isolates, respectively. This classification was supported by DNA–DNA hybridization studies, whereby a psychrotolerant isolate (strain U2/3T) showed 41.0 and 17.8 % relatedness to the type strains of the mesophilic species Mo
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Roth, Andreas, Marga Fischer, Mohamed E. Hamid, Sabine Michalke, Wolfgang Ludwig, and Harald Mauch. "Differentiation of Phylogenetically Related Slowly Growing Mycobacteria Based on 16S-23S rRNA Gene Internal Transcribed Spacer Sequences." Journal of Clinical Microbiology 36, no. 1 (1998): 139–47. http://dx.doi.org/10.1128/jcm.36.1.139-147.1998.

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Interspecific polymorphisms of the 16S rRNA gene (rDNA) are widely used for species identification of mycobacteria. 16S rDNA sequences, however, do not vary greatly within a species, and they are either indistinguishable in some species, for example, in Mycobacterium kansasii and M. gastri, or highly similar, for example, in M. malmoense and M. szulgai. We determined 16S-23S rDNA internal transcribed spacer (ITS) sequences of 60 strains in the genus Mycobacterium representing 13 species (M. avium, M. conspicuum, M. gastri, M. genavense, M. kansasii,M. malmoense, M. marinum, M. shimoidei, M. si
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Clawson, Michael L., Jeffrey Gawronski, and David R. Benson. "Dominance ofFrankiastrains in stands ofAlnus incanasubsp.rugosaandMyrica pensylvanica." Canadian Journal of Botany 77, no. 9 (1999): 1203–7. http://dx.doi.org/10.1139/b99-070.

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To address issues of dominance and diversity of Frankia spp. strains, we sequenced 16S rRNA genes from root nodules and strains collected from Alnus incana subsp. rugosa (Du Roi) R.T. Clausen and Myrica pensylvanica Loisel. stands. Of 22 strains isolated previously from A. incana, 16 had the same partial rDNA sequence; the remaining 6 strains composed five additional groups. The groups identified by 16S rDNA analysis corresponded to phenotypic groups established previously by one- and two-dimensional polyacrylamide gel analysis, colony and hyphal morphology, and carbon source utilization patte
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YADAV, AKHILESH, ASHA LATA SINGH, GOVIND KUMAR RAI, and MAJOR SINGH. "Assessment of Molecular Diversity in Chickpea (Cicer arietinum L.) Rhizobia and Structural Analysis of 16S rDNA Sequences from Mesorhizobium ciceri." Polish Journal of Microbiology 62, no. 3 (2013): 253–62. http://dx.doi.org/10.33073/pjm-2013-033.

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Molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (SSR) and 16S rDNA-RFLP markers. Phenotypic characterization with special reference to salinity and pH tolerance was performed. These isolates were identified as different strains of Mesorhizobium, Rhizobium, Bradyrhizobium, and Agrobacterium. Twenty SSR loci of Mesorhizobium ciceri, distributed across the other rhizobial genome, clearly differentiated 19 rhizobial isolates. Analogous clustering supported the results of 16S rDNA sequence-based phylogeny. Analysis of the 16S rDNA seque
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Vetriani, Costantino, Holger W. Jannasch, Barbara J. MacGregor, David A. Stahl, and Anna-Louise Reysenbach. "Population Structure and Phylogenetic Characterization of Marine Benthic Archaea in Deep-Sea Sediments." Applied and Environmental Microbiology 65, no. 10 (1999): 4375–84. http://dx.doi.org/10.1128/aem.65.10.4375-4384.1999.

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ABSTRACT During the past few years Archaea have been recognized as a widespread and significant component of marine picoplankton assemblages and, more recently, the presence of novel archaeal phylogenetic lineages has been reported in coastal marine benthic environments. We investigated the relative abundance, vertical distribution, phylogenetic composition, and spatial variability ofArchaea in deep-sea sediments collected from several stations in the Atlantic Ocean. Quantitative oligonucleotide hybridization experiments indicated that the relative abundance of archaeal 16S rRNA in deep-sea se
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Li, Heling, Zhigang Chen, Qing Ning, Faliang Zong, and Hong Wang. "Isolation and Identification of Morganella morganii from Rhesus Monkey (Macaca mulatta) in China." Veterinary Sciences 11, no. 5 (2024): 223. http://dx.doi.org/10.3390/vetsci11050223.

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A bacterium was isolated and identified from the secretion of a rhesus monkey with endometritis. The morphological results showed that the strain exhibited round, convex, gray-white colonies with smooth surfaces and diameters ranging from 1 to 2 mm when cultured on Columbia blood agar at 37 °C for 24 h; on salmonella–shigella agar (S.S.) at 37 °C for 24 h, the colonies appeared round, flat, and translucent. Gram staining showed negative results with blunt ends and non-spore-forming characteristics. Molecular biology results showed that the 16S rRNA sequence of the strain revealed over 96.9% si
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Jamieson, Barrie G. M., Simon Tillier, Annie Tillier, et al. "Phylogeny of the Megascolecidae and Crassiclitellata (Annelida, Oligochaeta): combined versus partitioned analysis using nuclear (28S) and mitochondrial (12S, 16S) rDNA." Zoosystema 24, no. 4 (2002): 707–34. https://doi.org/10.5281/zenodo.4524860.

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Jamieson, Barrie G. M., Tillier, Simon, Tillier, Annie, Justine, Jean-Lou, Ling, Edmund, James, Sam, Mcdonald, Keith, Hugall, Andrew F. (2002): Phylogeny of the Megascolecidae and Crassiclitellata (Annelida, Oligochaeta): combined versus partitioned analysis using nuclear (28S) and mitochondrial (12S, 16S) rDNA. Zoosystema 24 (4): 707-734, DOI: 10.5281/zenodo.4524860
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González, José M., Rafel Simó, Ramon Massana, et al. "Bacterial Community Structure Associated with a Dimethylsulfoniopropionate-Producing North Atlantic Algal Bloom." Applied and Environmental Microbiology 66, no. 10 (2000): 4237–46. http://dx.doi.org/10.1128/aem.66.10.4237-4246.2000.

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ABSTRACT The bacteria associated with oceanic algal blooms are acknowledged to play important roles in carbon, nitrogen, and sulfur cycling, yet little information is available on their identities or phylogenetic affiliations. Three culture-independent methods were used to characterize bacteria from a dimethylsulfoniopropionate (DMSP)-producing algal bloom in the North Atlantic. Group-specific 16S rRNA-targeted oligonucleotides, 16S ribosomal DNA (rDNA) clone libraries, and terminal restriction fragment length polymorphism analysis all indicated that the marine Roseobacter lineage was numerica
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Verma, Subhash Chandra, Soumitra Paul Chowdhury, and Anil Kumar Tripathi. "Phylogeny based on 16S rDNA andnifHsequences ofRalstonia taiwanensisstrains isolated from nitrogen-fixing nodules ofMimosa pudica, in India." Canadian Journal of Microbiology 50, no. 5 (2004): 313–22. http://dx.doi.org/10.1139/w04-020.

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Bacterial symbionts present in the indeterminate-type nitrogen (N)-fixing nodules of Mimosa pudica grown in North and South India showed maximum similarity to Ralstonia taiwanensis on the basis of carbon-source utilization patterns and 16S rDNA sequence. Isolates from the nodules of M. pudica from North India and South India showed identical ARDRA (Amplified Ribosomal DNA Restriction Analysis) patterns with Sau3AI and RsaI, but AluI revealed dimorphy between the North Indian and South Indian isolates. Alignment of 16S rDNA sequences revealed similarity of North Indian isolates with an R. taiwa
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