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1
Field, Claudia Louise. "Focusing the lens : the role of travel and photography in the personal and working lives of Vanessa Bell and Duncan Grant." Thesis, University of Sussex, 2015. http://sro.sussex.ac.uk/id/eprint/57242/.
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This thesis addresses how the photographic image contributed to the formation of the public and private identities of the artists Vanessa Bell and Duncan Grant. I propose that Bell and Grant primarily conceptualised photography as a medium of movement and it is this element that defines photographic images of them and their circle. Further, I suggest this definitive photographic element of their work situates them and the Bloomsbury Group in the development of English modernism in a new way. Chapter One explores the presence of movement in travel and tourism related photographic images from Bell and Grant's own generation and previous generations in their families. It compares images of alpine adventures, colonial life and first journeys to Europe alongside sections of personal correspondence by both generations offering a ‘verbal sketch' of the sights and sounds of the travel experience. Chapter Two considers how the photographic reproduction informs the development of public identity through an analysis of how Bell, Grant, Clive Bell and Julia Margaret Cameron used photographic images in the public arena and how contemporary media used photographs in assessments of their work. Chapter Three focuses on the nature of private physical and psychological photographic exchanges among both Julia Margaret Cameron's circle and the Bloomsbury Group and looks at paintings by Bell and Grant that were inspired by personal and private photographs in their possession. Chapter Four examines how the visual expression of monumentality and movement in photographs taken by Bell, Grant and their predecessors demonstrates a clear interest in making connections with past artistic and photographic traditions. The culmination of this discussion identifies defining features of the Bloomsbury photograph as created by Vanessa Bell and shows how it incorporates movement as a primary element of her photographic aesthetic.
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Marshall, Francis. "RB Kitaj and the idea of Europe." Thesis, University of Sussex, 2017. http://sro.sussex.ac.uk/id/eprint/67390/.
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This thesis analyses European themes in the work of the American painter RB Kitaj. It focuses most closely on the 1960s, a relatively under-researched period of his work, certainly compared with the 1970s and 80s, in part because most of the existing literature follows Kitaj's reading of his own oeuvre. Using canvases from the 1960s as examples, the thesis examines Kitaj's concerns with the history of the European Left prior to World War II. Study of these paintings reveals how, even at this early stage of his career, Kitaj conflated autobiography and history. A comparison of Kitaj's published and draft texts, written during and after these paintings were made, shows him altering their meaning according to his current concerns. This, in turn, shows how his revisions influenced later scholars' readings. Furthermore, due attention is given to two important, though often overlooked, bodies of work from the 1960s: the screenprints and the installation made at Lockheed for the Los Angeles County Museum of Art. Both reveal a sustained engagement with European themes, such as the Industrial Revolution, Modernism and its legacies, and Jewish history. Whereas Kitaj emphasised the centrality of Judaism to his work throughout the 1970 and 80s, he downplayed his concern with technology and Modernism, although both continued to inform his imagery until well into the 1980s. His shift away from new technology (eg photo-screenprinting) and a Modernist aesthetic, in favour of life drawing, is analysed against contemporary artistic debates in Britain, together with his fascination with the evolving history of the European Left during the 1970s. Kitaj's work reveals a sustained but constantly modulating, at times conflicted, meditation on European history and culture from an American perspective. In the final analysis, however, his engagement with Europe is, perhaps, the result of a spiritual and psychological impulse rooted in his personal and family history.
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Watkins, Aaron Emery. "A New Perspective on Galaxy Evolution From the Low Density Outskirts of Galaxies." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1499354382030385.
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Georgiev, Kristiyan. "GALATEA_RESETAct2.mp4." Diss., Temple University Libraries, 2014. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/296060.
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Computer and Information Science<br>Ph.D.<br>Robot mapping is a major field of research in robotics. Its basic task is to combine (register) spatial data, usually gained from range devices, to a single data set. This data set is called global map and represents the environment, observed from different locations, usually without knowledge of their positions. Various approaches can be classified into groups based on the type of sensor, e.g. Lasers, Microsoft Kinect, Stereo Image Pair. A major disadvantage of current methods is the fact, that they are derived from hardly scalable 2D approaches that use a small amount of data. However, 3D sensing yields a large amount of data in each 3D scan. Autonomous mobile robots have limited computational power, which makes it harder to run 3D robot mapping algorithms in real-time. To remedy this limitation, the proposed research uses mid-level geometric features (lines and ellipses) to construct 3D geometric primitives (planar patches, cylinders, spheres and cones) from 3D point data. Such 3D primitives can serve as distinct features for faster registration, allowing real-time performance on a mobile robot. This approach works in real-time, e.g. using a Microsoft Kinect to detect planes with 30 frames per second. While previous approaches show insufficient performance, the proposed method operates in real-time. In its core, the algorithm performs a fast model fitting with a model update in constant time (O(1)) for each new data point added to the model using a three stage approach. The first step inspects 1.5D sub spaces, to find lines and ellipses. The next stage uses these lines and ellipses as input by examining their neighborhood structure to form sets of candidates for the 3D geometric primitives. Finally, candidates are fitted to the geometric primitives. The complexity for point processing is O(n); additional time of lower order is needed for working on significantly smaller amount of mid-level objects. The real-time performance suggests this approach as a pre-processing step for 3D real-time higher level tasks in robotics, like tracking or feature based mapping. In this thesis, I will show how these features are derived and used for scene registration. Optimal registration is determined by finding plane-feature correspondence based on mutual similarity and geometric constraints. Our approach determines the plane correspondence in three steps. First step computes the distance between all pairs of planes from the first scan to all pair of planes from the second scan. The distance function captures angular, distance and co-planarity differences. The resulting distances are accumulated in a distance matrix. The next step uses the distance matrix to compute the correlation matrix between planes from the first and second scan. Finally plane correspondence is found by finding the global optimal assignment from the correlation matrix. After finding the plane correspondence, an optimal pose registration is computed. In addition to that, I will provide a comparison to existing state-of-the-art algorithms. This work is part of an industry collaboration effort sponsored by the National Institute of Standards and Technology (NIST), aiming at performance evaluation and modeling of autonomous navigation in unstructured and dynamic environments. Additional field work, in the form of evaluation of real robotic systems in a robot test arena was performed.<br>Temple University--Theses
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Maninger, Silke. "Genetic analysis of M94 of murine cytomegalovirus." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-122551.
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Mba, Mintsa Léa. "Synthèse et évaluation angiogénique d'analogues du M6P." Thesis, Montpellier, Ecole nationale supérieure de chimie, 2015. http://www.theses.fr/2015ENCM0028.
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L’angiogenèse, est le garant de l’intégrité vasculaire, grâce à la formation de nouveaux vaisseaux sanguins à partir des préexistants. Cette néovascularisation est régulée par des facteurs angiogéniques. Plusieurs stimuli participent au déséquilibre de la balance angiogénique, une vascularisation anormale en résulte. L’intérêt des thérapies actuelles est de restaurer une vascularisation normale, par ciblage des facteurs impliqués dans le processus angiogénique. Il fut démontré que le RM6P-CI est partie prenante dans ce mécanisme. Il a la particularité d’interagir avec plusieurs ligands, dont le M6P et ses dérivées.Il est question de savoir si le M6A, un dérivé isostère du M6P, se lie au RM6P-CI. Pour cela, le test CAM légèrement modifié, a été réapproprié pour confirmer premièrement l’activité pro-angiogénique du M6A. Deuxièmement la technique de cytométrie en flux est utilisée pour mettre en évidence l’interaction entre le M6A et le RM6P-CI<br>Angiogenesis is the protector of vascular integrity, through the formation of new blood vessels from pre-existing vessels. The angiogenesis is regulated by the angiogenic factors. Several stimuli are involved in the angiogenic balance’s destabilization, which produces an unusual vascularization. The interest of conventional and targeted therapies is to restore a normal vascularization, by targeting all the factors which are involved in the angiogenic process. It has been demonstrated that the CI-M6PR is involved in this mechanism. This receptor has the distinction of having more binding sites, so a variety of ligands, including the M6P and its analogs. During this study we want to know if the M6P, isostere analog of M6P, interacts with the CI-M6PR. For this, we reappropriated the assay CAM, slightly modified, to confirm at first the pro-angiogenic activity of M6A. In a second step we will use the flow cytometry technique to highlight the interaction between the M6A and CI- M6PR
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Sippelli, Simona. "Nouvelle Voie d'isolement du RM6P : Biosynthèse et synthèse de dérivés du M6P." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS157.
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Le récepteur cation-indépendant du mannose-6-phosphate (CI-RM6P) est une glycoprotéine transmembranaire impliquée dans de nombreux processus biologiques comme le transport des enzymes lysosomales vers les lysosomes et aussi dans le phénomène connu comme l’angiogenèse. Les analogues du M6P se sont avérés être des effecteurs de l’angiogenèse tumorale. La synthèse des nouveaux dérives bidentés, fonctionnalisés avec des analogues du M6P, ouvre la voie à une nouvelle méthode pour isoler le CI-RM6P.Ces “antennes biologiques” seront ainsi utilisées pour étudier leurs affinités vis-à-vis du CI-RM6P. En perspective, une nouvelle classe de dérivés de Sepharose, fonctionnalisée par nos ligands bidentés, vont être générée pour leurs emplois dans les traditionnels techniques de purifications des protéines<br>The cation-indipendent mannose-6-phosphate receptor (CI-MPR) is a trans membrane glycoprotein implicated in numerous biological processes such as the transporting of the lysosomal enzymes to the lysosomes and in the phenomenon known as angiogenesis.The analogues of M6P have proven themselves to be effectors of tumour angiogenesis.The synthesis new bidentates derivatives, functionalised with analogues derivatives of M6P, opens the way to a new method to isolate the CI-MPR.These “biological antennae” will be used to study binding affinity with the receptor CI-MPR. In prospective, a new class of Sepharose derivatives, functionalised with ours bidentates ligands, will be generated to be used in the traditional technique of purifying proteins
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Daum, Magnus. "Cryptanalysis of Hash functions of the MD4-family." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=97642777X.
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Bautista, Gabriel, Oscar Castañeda, la Flor Jorge De, and Anthony Macedo. "Física (MA64): Guía de laboratorio, ciclo 2014-2." Universidad Peruana de Ciencias Aplicadas - UPC, 2014. http://hdl.handle.net/10757/323802.
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El desarrollo de las habilidades experimentales de
un estudiante de Arquitectura es una parte
importante de su formación. Un profesional en arqui
tectura debe saber medir, usar
correctamente las unidades, realizar apreciaciones
y generar conclusiones sobre ocurrencias
reales, redactar adecuada y correctamente un inform
e, etc. Estas habilidades habrán de ser
adquiridas y afianzadas durante el desarrollo de su
s cursos formativos dentro de la
Universidad Peruana de Ciencias Aplicadas.
Dado que la Física es esencialmente experimental, e
s evidente que será precisa para el
aprendizaje y desarrollo de tales características.
Por esta razón es que consideramos necesaria
la realización de esta guía de laboratorio en que s
e da un especial tratamiento de la parte
experimental de la Física, contando con el debido s
ustento teórico de los experimentos
escogidos, de modo tal que los estudiantes desarrol
len de una manera organizada y
consciente, las pocas, pero sustanciales, experienc
ias de laboratorio abordadas, y lleguen así a
hacer suyos los conceptos que les serán de gran uti
lidad no sólo en el afianzamiento de sus
conocimientos del curso de Física, sino también en
su futura actividad profesional.
10
Bautista, Gabriel, Oscar Castañeda, la Flor Jorge De, and Anthony Macedo. "Física (MA64): Guía de laboratorio, ciclo 2015-0." Universidad Peruana de Ciencias Aplicadas - UPC, 2014. http://hdl.handle.net/10757/337107.
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El desarrollo de las habilidades experimentales de un estudiante de Arquitectura es una parte importante de su formación. Un profesional en arquitectura debe saber medir, usar correctamente las unidades, realizar apreciaciones y generar conclusiones sobre ocurrencias reales, redactar adecuada y correctamente un informe, etc. Estas habilidades habrán de ser adquiridas y afianzadas durante el desarrollo de sus cursos formativos dentro de la Universidad Peruana de Ciencias Aplicadas.
Dado que la Física es esencialmente experimental, es evidente que será precisa para el aprendizaje y desarrollo de tales características. Por esta razón es que consideramos necesaria la realización de esta guía de laboratorio en que se da un especial tratamiento de la parte experimental de la Física, contando con el debido sustento teórico de los experimentos escogidos, de modo tal que los estudiantes desarrollen de una manera organizada y consciente, las pocas, pero sustanciales, experiencias de laboratorio abordadas, y lleguen así a hacer suyos los conceptos que les serán de gran utilidad no sólo en el afianzamiento de sus conocimientos del curso de Física, sino también en su futura actividad profesional.
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Montuori, Giulia. "The m6A RNA modification sustains neuroblastoma tumour aggressiveness." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/276989.
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The N6-methyladenosine, also known as m6A, is the most common post-transcriptional modification in mRNAs and long non-coding RNAs and that profoundly influences mRNA biology, from early processing in the nucleus to final steps of translation and decay in the cytoplasm. Taking into consideration the importance of RNA in shaping cell fate, m6A is widely recognized as an additional layer in the regulation of gene expression, also thanks to its dynamic and reversible nature. Therefore, it is not surprising that any misregulation in m6A content might lead to the loss of cellular homeostasis. This effect is particularly evident when it comes to stem cells differentiation, embryo development and cancer. In a tumorigenic context, the m6A could affect the development, progression, cancer stem cells (CSCs) renewal and drug resistance of solid and liquid tumours. So, the m6A is consistently becoming a new attractive pharmacological target.
Neuroblastoma (NB) is a neuroendocrine tumour of early childhood that derives from undifferentiated cells of the sympathoadrenal lineage of the neural crest. About 50% of patients have a very aggressive form of NB, with an overall survival rate of less than 30% despite heavy treatments. Moreover, NB is a challenging druggable tumour due to a low rate of somatic mutations. Somatic mutations at significant frequency have been identified in only five genes that also show detectable expression. Among these, only one is currently a directly validated druggable target.
Two m6A regulators (METTL14 and ALKBH5) are aberrantly expressed in high-risk NB patients, and their alteration in NB cell lines affects tumour aggressiveness. Specifically, the overexpression of the methyltransferase METTL14 increases cell proliferation and invasion in vitro and tumour growth in mice acting as an oncogene, while ALKBH5 restoration affects cell proliferation, apoptosis and invasion in an opposite fashion. Importantly, the demethylase ALKBH5 impaired tumour formation in vivo when costitutively expressed and dramatically slows down tumor progression in mice when is induced by causing massive apoptosis. These data suggest that ALKBH5 acts as a potent tumour suppressor in NB.
We discovered that METTL14 and ALKBH5 exert their effect on different levels by affecting mRNA stability or translation, respectively. Although the contribution to NB of the altered stability of transcripts related to mRNA processing in METTL14-overexpressing cells is less understand, the increase translation of pro-apoptotic genes in the ALKBH5-overexpression condition leaves little doubts.
Our results unveil the m6A and its regulators as potential therapeutic targets for treating NB. Indeed, in collaboration with the Laboratory of Genomic Screening of Professor Alessandro Provenzani, we presented an encouraging proof-of-concept of the reader YTHDF1 as a possible pharmacological target.
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Monasterio, Schrader Patricia Irene de [Verfasser], Klaus-Armin [Akademischer Betreuer] Nave, Gregor [Akademischer Betreuer] Bucher, and Dieter [Akademischer Betreuer] Klopfenstein. "Characterization of the neuronal proteolipids M6A and M6B and the oligodendroglial tetraspans PLP and TSPAN2 in neural cell process formation / Patricia Irene de Monasterio Schrader. Gutachter: Klaus-Armin Nave ; Gregor Bucher ; Dieter Klopfenstein. Betreuer: Klaus-Armin Nave." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2012. http://d-nb.info/1043028757/34.
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Can, Sermet. "Dissecting domain-specific functions of the IGF2/M6P receptor." Thesis, University of Oxford, 2016. http://ora.ox.ac.uk/objects/uuid:63ee7b6a-a619-4d42-973e-e888dc427378.
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The P-type lectin Mannose 6-phosphate/Insulin-like growth factor 2 receptor (M6P/IGF2R) is a type I trans-membrane protein with 15 homologous extracellular domains that bind several ligands. While domains 3, 5 and 9 bind to Mannose 6-Phosphate (M6P), domain 11 binds to IGF2 (Insulin-like growth factor 2). In mice, igf2r expression is monoallelic and full knock-out of the receptor maternal allele (Igf2r<sup>-m/+p</sup>) results in developmental abnormalities and neonatal lethality, marking the significance of the receptor in organogenesis and postnatal viability. What remains unknown is the contribution of each of these domains into M6P/IGF2R's roles in development. My aims are dissecting M6P and IGF2 binding domains' functions in the context of full length receptor in vitro and investigating Domain 11 loss-of-function in vivo. In this thesis, SPR analysis of full-length M6P/IG2R mutants showed that M6P binding domains function independent of each other, and domain 3 is the dominant M6P binding domain at physiological pH. Introducing gain-of-function mutations to domain 11 binding site revealed presence of inter-loop interactions and inhibitory residues in CD and FG loop in the context of the full-length receptor. I1572A results in domain 11 loss of-function mutation with preserved binding of other ligands, suggesting that I1572A (or its homolog mutations in other mammals) could be utilized in in vivo studies to dissect domain 11 functions. In mice, maternal transmission of Domain 11 loss-of-binding function mutation (I1565A, mouse homolog of I1572A) resulted in increased birth weight and substantially reduced postnatal survival (~30%). The disproportional cardiomegaly and disorganised myocardium in Igf2r<sup>I1565Am/+p</sup> neonates suggest that postnatal lethality is due to cardiac insufficiency. Furthermore, placental and embryonic oedema in Igf2r<sup>I1565Am/+p</sup> mice is indicative of the presence of prenatal congestive cardiac failure, beginning as early as at embryonic day 14.5. In addition, surviving females developed uterine distension due to vaginal atresia. Overall, these results show that domain 11 loss-of-function, on its own, is the primary reason for the most of abnormalities that is previously reported in the full knock out mice. Thus, domain 11 is the primary domain for M6P/IGF2R's roles in development.
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van, den Brink Nemo. "Chemical abundances in main-sequence stars in open cluster M67." Thesis, Uppsala universitet, Teoretisk astrofysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-169017.
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The discovery of a solar twin in the open star cluster M67 (Önehag et al. 2011) implies a near-solar chemical composition for the cluster. This study uses high-resolution spectroscopic data of five main-sequence stars in M67 to analyze their abundance of a few key elements and compare results to the solar-twin composition and the composition of field twins (Melendez et al. 2009). The derived composition was also compared to predictions of stellar-structure models including the effects of element diffusion. (Richard, private communication). It is found that all analyzed elements are, to varying degree, less abundant in the five main sequence stars than in the solar twin. With the possible exception of iron, all derived abundances also fall clearly below the diffusion predictions.
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Yu, Keyan. "Seismic vulnerability assessment for Montreal-an application of HAZUS-MH4." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103535.
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Seismic loss estimation for Montreal, Canada is performed for a 2% in 50 years seismic hazard using the HAZUS-MH4 tool developed by US Federal Emergency Management. The software is manipulated to accept a Canadian setting for the Montreal study region, which includes 522 census tracts. The accuracy of loss estimations using HAZUS is dependent on the quality and quantity of data collection and preparation. The data collected for Montreal study region comprise: 1) the building inventory 2) hazard maps regarding soil amplification, liquefaction, and landslides 3) population distribution at three different times of the day 4) census demographic information and 5) synthetic ground motion contour maps using three different ground motion prediction equations. All these data are prepared and assembled into geodatabases that are compatible with the HAZUS software. The study estimated that roughly 5% of the building stock would be damaged with direct economic losses evaluated at 1.4 billion dollars for a scenario corresponding to the 2% in 50 years scenario. The maximum number of casualties associated with this scenario corresponds to a time of occurrence of 2pm and would result in approximately 500 people being injured. Epistemic uncertainty was considered by obtaining damage estimates for three attenuation functions that were developed for Eastern North America. The results indicate that loss estimates are highly sensitive to the choice of the attenuation function and suggests that epistemic uncertainty should be considered both for the definition of the hazard function and in loss estimation methodologies. The next steps in the study should be to increase the size of the survey area to the Greater Montreal which includes more than 3 million inhabitants and to perform more targeted studies for critical areas such as downtown Montreal, and the south-eastern tip of Montreal. The current study was performed mainly for the built environment; the next phase will need to include more information relative to lifelines and their impact on risks.<br>Une analyse de risques sismiques est effectuée pour Montréal, pour un scénario de tremblement de terre correspondant à un aléa de 2% en 50 ans avec le logiciel HAZUS-MH4 développé par le FEMA (Federal Emergency Management Agency). Les fichiers d'entrée des données ont été adaptés afin d'accepter les données pour la région de Montréal. L'analyse est effectuée en discrétisant le territoire selon les secteurs de recensement, soit 522 au total. La précision des estimations sur les conséquences d'un séisme dépend de la qualité et la quantité des données compilées. Les données recueillies pour la présente étude sur la région de Montréal incluent: 1) l'inventaire des bâtiments 2) les cartes de risques pour les effets de site, la liquéfaction et les glissements de terrain et 3) la répartition de la population à trois moments différents de la journée 4) le recensement démographique et 5) les cartes des mouvements du sol pour trois différentes équations de prédiction. Toutes ces données ont préparées et compilées dans des bases de données géo-référencées en format compatible avec le logiciel HAZUS. L'étude indique qu'environ 5% du parc immobilier serait endommagé pour des pertes économiques directes de 1,4 milliards de dollars. Le nombre de victimes maximum est associé avec un scénario d'occurrence à 14:00 heures avec environ 500 personnes blessées. L'incertitude épistémique a été considérée en considérant trois modèles d'atténuation proposés dans la littérature pour l'est de l'Amérique du Nord. Les résultats indiquent que les risques sont très sensibles à l'incertitude épistémique et il est recommandé de considérer cette incertitude autant dans les études de risque que pour les analyses de l'aléa sismique. Les prochaines étapes d'un projet d'évaluation des risques devrait étendre l'étude à la grande région métropolitaine et cibler des secteurs critiques tels que le centre-ville et le sud-est de l'île de Montréal. La présente étude est limitée aux dommages aux bâtiments. Il serait important de modéliser la vulnérabilité des lignes de vie et de quantifier leur impact sur l'estimation des risques.
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Conibear, Elizabeth. "Sorting signals for the recycling of the M6P/IGFII receptor." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260480.
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McNeer, Sarah Kathryn. "Investigation of Dinitroparaben-Induced Apoptosis in M624 Human Melanoma Cells." Marietta College Honors Theses / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=marhonors162007332615959.
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Amcoff, Patric. "The role of thiamine in Baltic salmon developing the M74-syndrome /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2000. http://epsilon.slu.se/avh/2000/91-576-5924-9.pdf.
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Tanguturi, Sai Kishan. "Effect on Contact Resistance dueto Cross Connection of MC4 Compatible Connector." Thesis, Högskolan Dalarna, Energiteknik, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:du-28838.
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Electrical connectors are the blocks that connect solar panels together. Whenever a photovoltaic plant commences, the main discussion goes around on solar panels, inverters, charge controllers, etc. But the topic of connectors is usually hardly discussed. Connectors in a photovoltaic system can definitely contribute to improve the overall performance of the system, provided that importance is given while selecting the connectors. The electrical connectors used in photovoltaic systems can be connected in two possible ways. Connectors can be connected either in a pure-connection or in a cross-connection. Male and female connectors from the same brand results a pure-connection (P-C). Male and female connectors from two different brands results in a cross-connection (C-C). There have been discussions in photovoltaic, electrical connector markets and international solar events regarding the risks involved, losses and consequences due to a cross-connection. The main reason behind cross-connections is the unawareness of the installers in knowing the difference between a pure-connection and a cross-connection. Even though the installers are aware of this difference, they are not aware of the consequences of cross-connections. Multi-Contact, a leading electrical connector manufacturer of MC4 photovoltaic connectors affected by the counterfeit products of MC4, due to the sudden boom in the solar market during 2011-12. With the help of TÜV Rheinland, Multi-Contact conducted couple of tests namely temperature increase test and accelerated stress tests to understand the disadvantages of cross-connections. This thesis tried to replicate the tests performed by Multi-Contact in an attempt to understand the test results by using connectors that are used in the Swedish market. Performing temperature increase test and accelerated stress tests on most commonly used connectors in the Swedish market is the main aim of this thesis. The first test, gives an understanding of the temperature variations across various connector sets (four connector sets from various manufacturers used in this thesis) and the latter tests helps to understand the quality of the contact resistance of these connector sets. The four connector set manufacturers used in this test were Multi-Contact (MC), Weidmüller (WM), Blussun solar (BSS) and PBM. The quality of contact resistance of a connector is directly related to the quality of the connector set. During the 20 minutes of the temperature increase test, the connector set from WM performed better than its competitors in the P-C. Whereas, the MC-BSS connector set had performed well in the C-C. The connector type of male MC and female BSS showed its dominance throughout the test. Unfortunately, no conclusions were able to be drawn from this test results due to insufficient information about the test procedure. From the results of accelerated stress tests, the C-C set from MC outperformed its P-C counterpart. All ten connector sets used in this project passed the standard and qualified as connectors with good quality contact resistance. Therefore the best results out of only a P-C connector set does not seems to be completely true. With the standard used in this thesis, it is quite difficult to judge the quality of connectors. Rather than saying a P-C is superior and a C-C is inferior in terms of quality, there is a need to come up with a new method to evaluate the quality of connectors. Matching the connectors based on their tolerances could be a potential solution to the mismatching problem in connectors.
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Lahbib, Mansais Yvette. "Structure et expression du genome du bacteriophagetempere mv4 de lactobacillus bulgaricus." Toulouse 3, 1989. http://www.theses.fr/1989TOU30093.
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Caracterisation moleculaire du bacteriophage mv4 de lactobacillus bulgaricus. Realisation d'une carte physique du genome. Clonage de l'adn du phage et etude de l'expression des genes clones. Des genes codant pour des proteines de structure et une lysine ont pu etre localises. Mise en evidence du site d'integration du phage dans le chromosome de souches lysogenes de lactobacillus. Etude comparative du phage mv4 avec 3 autres phages temperes et 4 phages virulents
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Combemale, Stéphanie. "Nano-objets mannosylés et nouveaux analogues du M6P : application à l'angiogenèse." Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20081.
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En 1971, Le Dr. Américain Judah Folkman a publié une hypothèse selon laquelle la croissance tumorale dépendait de l'angiogenèse. Le défit des recherches actuelles est de trouver un moyen pour affamer la tumeur en inhibant son angiogenèse. L'angiogenèse est un processus physiologique complexe qui fait intervenir de nombreux récepteurs, parmi lesquels se trouve le récepteur du mannose-6-phosphate / Insulin-like growth factor II (RM6P/IGFII). Le but de ce travail a donc consisté en la synthèse de nano-objets mannosylés et de nouveaux analogues du Mannose-6-Phosphate (M6P) puis en l'évaluation de leur activité angiogénique par la méthode de la « CAM » sur des embryons de poulet et dans le modèle des explants d'aorte de rat. Dans un premier temps des nanoparticules d'or ont été préparées, fonctionnalisées avec les dérivés du M6P ayant montré des résultats intéressants au cours de travaux antérieurs réalisés au sein du laboratoire. Par la suite des analogues originaux tels que un borate, un acide boronique, un pyrophosphate, un pyrophosphonate ainsi que l'analogue rétroisotère du M6P ont été synthétisés. D'autre part, il a été montré que le RM6P/IGFII pouvait lier deux molécules de M6P ou une molécule d'oligosaccharide diphosphorylée par monomère. De ce fait, des molécules bidentées, des composés de type bolaforme et des trisaccharides difonctionnalisés ont été élaborés puis leur activité biologique a été valuée<br>In 1971, the American Dr. Judah Folkman published the hypothesis : tumor growth depends on angiogenesis. The challenge of current research is to find a way to starve tumors by inhibiting angiogenesis. Angiogenesis is a complex physiological process that involves many receptors, among which is the receptor for mannose-6-phosphate / Insulin-like growth factor II (RM6P/IGF-II). The aim of this work was the synthesis of mannosyl nano-devices and new analogues of Mannose-6-Phosphate (M6P). Evaluation of their angiogenic activity was made by the 'CAM essay' on embryo of chicken and in the model of the explantations of rat's aorta. First, gold nanoparticles functionnalized with M6P analogues were prepared. Those analogues have been chosen among the most interesting candidates as described previously in our laboratory. Secondly, original analogues such as a borate, a boronic acid, a pyrophosphate, a pyrophosphonate as well as the retroisotere analogue of the M6P were synthetized. The RM6P/IGFII can bind two molecules of M6P or a diphosphoryled oligosaccharide molecule by monomer (receptor). Therefore, bidentate molecules, difunctionnalized bolaform compounds and difunctionnalized trisaccharides were synthesized and their biological activity evaluated
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Tobin, Ryan Wesley. "Photometric variability of Sun-like stars in the old open cluster M67." [Ames, Iowa : Iowa State University], 2008.
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Trécant, Claire. "Synthèses du M6G (morphine-6-glucuronide) et d'analogues potentiellement actifs en analgésie." Clermont-Ferrand 2, 2008. http://www.theses.fr/2008CLF22008.
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Les travaux décrivent le développement d'un métabolite actif de la morphine, le morphine-6-glucuronide (M6G). Ce dernier présente une activité plus élevée que la morphine dans le traitement de la douleur avec des effets secondaires moindres. La première partie est consacrée à la mise au point d'une synthèse originale et applicable à l'échelle semi-industrielle du M6G. La stratégie adoptée est basée sur une glycosylation, réaction qui a été optimisée par l'étude de différents paramètres. La seconde partie présente la préparation d'analogues lipophiles du M6G qui ont été testés en pharmacologie pour l'évaluation de leur potentialité antinociceptive. Nous avons ainsi substitué la fonction acide carboxylique du M6G par différents groupes : tétrazole, 1,3,4-oxadiazole, 1, 2, 4-triazole et amide. Les résultats préliminaires obtenus démontrent une activité supérieure à celle du M6G et encouragent la poursuite des recherches sur ce sujet
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Ahlvind, Julia. "Isochrone and chemical ages of stars in the old open cluster M67." Thesis, Uppsala universitet, Observationell astrofysik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-434634.
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The open cluster Messier 67 is known to have chemical composition, metallicity and age (~ 4 Gyr) close to the Sun. Therefore, it is advantageous for stellar physical studies and of stellar evolution, in particular for solar like stars within the cluster. This work considers three such stars, the formerly studied solar twin M67-1194 and two more recently suggested solar twins M67-1787 & 2018. Most solar twins show a ratio of volatile to refractory elements that systematically depart from the Sun’s. Our targets do not follow this trend as closely. Their composition is closer to the Sun and they are, therefore, exquisite targets for studies of stellar evolution within the cluster. However, their solar likeness also provides studies regarding the origin and evolution of the Solar system. The stellar ages of the solar twins are established through a chemical clock [Y/Mg] and via stellar isochrones from BaSTI. The latter age assessment of the solar twins is supplemented with the analysis of two subgiant stars M67-1442 & 1844. We approach the isochrone-based method using spectroscopically, astrometrically and photometrically derived parameters. The different ages of the stars and methods thus estimate the age of the cluster itself. The chemical ages of the stars suggest a cluster age of 4.56 ±0.44 Gyr and the isochrone-based estimates suggests a cluster age within the range 3.30-5.51 Gyr. Our results thus affirm and imply a near solar age of the cluster.
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Muniz, Jeferson de Lima. "Mk4 : programa para síntese de funções majoritárias com até 4 variáveis de entrada /." Ilha Solteira, 2019. http://hdl.handle.net/11449/180711.
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Orientador: Alexandre César Rodrigues da Silva<br>Resumo: Com a evolução da tecnologia, os CIs (Circuitos Integrados) com tecnologia CMOS (Complementary Metal-Oxide Semicondutor) têm se tornado cada vez menores e mais eficientes, entretanto, esta tecnologia está atingindo os limites físicos. Para minimizar ainda mais os circuitos digitais, novas tecnologias foram desenvolvidas como, por exemplo, a tecnologia QCA (Quantum-Dot Cellular Automata) que em conjunto com a lógica majoritária tem despertado o interesse da comunidade acadêmica no que se refere ao desenvolvimento de ferramentas de síntese e de otimização. Neste trabalho implementou-se o programa denominado MK4 que tem como proposta realizar a minimização de funções majoritárias com até quatro variáveis, utilizando as ideias contidas no mapa de Karnaugh. Os resultados obtidos pelo MK4 foram comparados com os do programa exact_mig. De 65.536 funções comparadas, 92,60% das funções geradas pelo programa MK4 tiveram custos iguais ou inferiores em relação as funções geradas pelo exact_mig.<br>Abstract: With the evolution of technology, the ICs (Integrated Circuits) with CMOS (Complementary Metal-Oxide Semiconductor) technology has become smaller and more efficient. However, this technology is reaching its physical limits. To further minimize digital circuits, new technologies are presented such as, QCA (\textit{Quantum-Dot Cellular Automata}) technology that together with majority logic has aroused the interest of the academic community in the development of synthesis and optimization tools. In this work the program denominated MK4 was implemented, with the purpose of minimizing majority functions with up to four variables, using the Karnaugh map. The results obtained by MK4 were compared with those of the exact_mig program. From 65,536 functions compared, 92.60% of the functions generated by the MK4 had equal or lower costs in relation to the functions generated by the exact_mig.<br>Mestre
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Peng, Peijing. "Interaction of alpha-gamma-MSH analogues with MC1, MC3 and MC4 melanocortin receptors." Thesis, University of Bath, 1997. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338413.
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Confort, Carole. "Etude d'une forme soluble du récepteur IGFII/M6P dans les cancers du sein." Montpellier 1, 1995. http://www.theses.fr/1995MON1T025.
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Richaud, Emmanuel. "L'Instruction budgétaire et comptable M14 et la gestion financière des petites collectivités locales." Corte, 2003. http://www.theses.fr/2003CORT1022.
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L'instruction M14 constitue un système réglementaire déterminant la gestion financière des communes et établissements de coopération intercommunale, notamment les petites collectivités, en vigueur depuis 1997. L'objectif était alors de rapprocher la tenue de leurs comptes du P. C. G. Et de donner une meilleure vision patrimoniale des collectivités. Mais l'analyse systémique démontre que l'irrespect des principes budgétaires et comptables détourne l'instruction de ses buts. Au contraire, l'application de cette instruction engendre l'entropie caractérisée par l'augmentation de l'impôt. Ce phénomène naît de facteurs intrinsèques à la M14, l'irrespect du principe d'unité en raison de la distinction entre section de fonctionnement et section d'investissement, ainsi que de l'introduction de l'amortissement, technique comptable à vocation fiscale. L'entropie constitue également la conséquence de facteurs externes à l'instruction, démontrant ainsi qu'il s'agit d'un système ouvert à des éléments interférents. Ces nouveaux éléments sont constitués par des systèmes normatifs propres à la collectivité ou extérieurs à celle-ci. Ils provoquent un blocage du système dont il découle une augmentation des charges. Parallèlement, l'instruction M14 s'avère inadaptée aux petites collectivités : la mise en œuvre d'une comptabilité patrimoniale apparaît particulièrement ardue en raison de l'hétérogénéité de leur patrimoine et de l'application inappropriée des techniques de l'amortissement et de la provision, donc de l'altération du principe de prudence. De même, la vision patrimoniale n'est que superficielle en raison des limites à l'analyse financière et à la consolidation de leurs comptes. Ce système est au contraire déséquilibré par l'incertitude des acteurs ainsi que par la complexité résultant d'éléments incertains. Ceci amène à s'interroger, au travers de l'activité de la D. G. C. P. , sur la pertinence de l'application actuelle du principe de séparation entre l'ordonnateur et le comptable<br>The M14 directive is a regulation, establishing the financial management of districts and intra-district coopérative institutions, notably of small-communities. This directive has been in force since 1997. At that time, the aim was to standardize book-keeping, according to the PGC, and to give a best patrimonial outlook of the districts. But, the methodical analyse reveals that the disrespect of financial and accounting principles, divert the directive from its aims
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Papakonstantinou, Nikolaos. "Examining LUMBA UVES pipeline spectroscopy on giant and sub-giant stars of M67." Thesis, Uppsala universitet, Observationell astrofysik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-448637.
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In this work, the efficiency of the LUMBA UVES pipeline for processing of spectroscopic observations is tested by use on 23 high-resolution spectra of the open star cluster M67. An abundance trend discovered by Gavel et al. (2019) concerning iron abundances of giant and sub-giant stars of that cluster is examined. An initial run for a set of ”Gaia FGK benchmark stars”, as described in Blanco-Cuaresma et al. (2014) and Heiter et al. (2015) helps inspect the structure, method and output of the pipeline. Through Python language programming, processes are greatly automatized and the pipeline is run for a total of 460 weak and strong iron lines of our 23-star sample. The line fitting and efficiency of the pipeline is appreciated by statistically analyzing the results and looking into individual discrepant ones. The abundance trend is reproduced while using FeI lines, unlike runs using FeII lines. Trends in abundance over line strength plots also hint at bias through the Gaia-Eso Survey (GES) microturbulence relation. Using internal Data Release 6 (iDR6) and LUMBA-derived starting parameters, log(g) - Teff plots of our sample stars agree with a previously established 4.3 Gyr cluster age. An alternate run is performed for those stars, using LUMBA-derived starting parameters. The choice of starting parameters does impact abundance derivation, but is not the primary source of persistent systematic discrepancies.
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Szkoda, Blake E. "THE EFFECTS OF CITRAL ON CASPASE-3 ACTIVATION IN M624 AND HaCaT CELLS." Marietta College Honors Theses / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1463566418.
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Husband, Paul W. Jr. "Identification and Characterization of Long Period Variable Stars in the Globular Cluster M69." Bowling Green State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1499418711065308.
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Oleryd, Josefin, and Karl-Johan Askevik. "Nätplan för fördelningsstation M4 Torsby." Thesis, Högskolan Väst, Avdelningen för data-, elektro- och lantmäteriteknik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hv:diva-9804.
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Det här examensarbetet är utfört på uppdrag av Kungälv Energi AB. Syftet med arbetet är att upprätta en nätplan för området M4 Torsby som kan användas som underlag till investeringar. Nätplanen innehåller en geografisk beskrivning som ger en överblick av markförhållanden, en tillståndskontroll av nätet vid normal- samt reservdrift, kontroll av nätets prestanda vid framtida lastökning samt åtgärdsförslag. M4 Torsby är ett landsbygdsnät bestående av fem linjer som matar totalt 1949 kunder. Beräkningar som utförts i Kungälv Energis beräkningsprogram dpPower visar att på linje L403 finns det en Cu 10 friledning som är överbelastad vid normaldrift. I nätet finns även överbelastade transformatorer. Reservdrift i området kan hanteras på alla linjer förutom på linje L401 vid det specifikt valda beräkningsfallet. Undersökning av nätets framtida prestanda har beräknats enligt Kungälv Energis och Kungälv Kommuns planer för området. Även extra last har adderats för att få en högre belastningsökning än de redan planerade. Resultaten visar att nätet klarar denna belastningsökning i normal- och reservdrift förutom då K406 reservmatar stor del av L401. En ACJJ50 kabel i Kärna centrum överbelastas. Arbetet resulterade i åtgärdsförslag för transformatorer samt placering av nya frånskiljare för bättre sektionering av nätet. Även ett åtgärdsförslag på den ACJJ50 kabel som kan bli överbelastad vid framtida reservdrift har framställts.<br>This thesis is made on behalf of Kungälv Energi AB. The purpose of the thesis is to establish a network plan for the area M4 Torsby that can be used as a foundation for reconstruction and investments in the area. The network plan contains a geographical description that provides an overview of the area including ground conditions and nature reserves. It also procures condition monitoring of the power network to detect its strengths and weaknesses in normal and reserve operation. To detect if the network can handle future loads a simulated scenario has been made. M4 Torsby consists of five lines which supply 1949 customers. Calculations show that a specific part of line L403 and some transformers around the area are overloaded during normal operation. The area can be alternately supplied. Calculations on the simulated future show that the network can handle future loads in both normal and reserve operation if the overloaded part of L403 is upgraded.
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Mowery, David Kaiser. "Use of a textile wastewater sludge as a supplemental solid fuel." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-11012008-063640/.
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Mangeshkar, Milan. "Estimation of partial group delay with applications to small samples." Diss., This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-10042006-143848/.
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Mangalvedhe, Nitin R. "An eigenstructure technique for direct sequence spread spectrum synchronization." Thesis, This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-03042009-040521/.
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Moo, Jason. "Applying human-computer interaction methodologies to the development of computer-aided design software." Thesis, This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-03042009-040630/.
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Meng, Ronald L. "Design of moment end-plate connections for seismic loading." Diss., This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-11082006-133629/.
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Mingin, Wendy E. "Two projects." Thesis, Virginia Tech, 1995. http://hdl.handle.net/10919/46410.
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The purpose of this thesis is not to begin with a premise and follow it through, but to discover through two different projects to what extent architecture can be understood, and to examine those "tools" of architecture that turned inspiration and conception into projects with architectural qualities.<br>Master of Architecture
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Tanco, Sebastián. "Insights into the specificity and function of M14 metallocarboxypeptidases from structural and degradomic studies." Doctoral thesis, Universitat Autònoma de Barcelona, 2012. http://hdl.handle.net/10803/117670.
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Las proteasas son enzimas que escinden proteínas catalizando la hidrólisis del enlace peptídico. Todas las proteínas sufren proteólisis en algún momento u otro de su ciclo de vida y de este modo las proteasas regulan casi todos los procesos biológicos. Las carboxipeptidasas son proteasas que hidrolizan el enlace C-terminal de las proteínas y péptidos. En esta tesis se estudia a las carboxipeptidasas de la familia M14 en la clasificación de MEROPS (y que describiremos como metalocarboxipeptidasas). Las metalocarboxipeptidasas controlan importantes procesos biológicos, como son la regulación de la presión sanguínea, el equilibrio entre coagulación y fibrinólisis, o el procesamiento de neuropéptidos y hormonas peptídicas. Además, están implicadas en procesos patológicos como el cáncer o las enfermedades neurodegenerativas.
La información disponible sobre la función biológica de estas enzimas es muy limitada, por lo que en la presente tesis se planteó como objetivo profundizar en el estudio de la función de varias metalocarboxipeptidasas. Con esta finalidad se realizaron ensayos cinéticos, estudios de biología celular y de determinación de la estructura tridimensional. Además, se desarrollaron y aplicaron distintas técnicas proteómicas para el estudio de los sustratos de carboxipeptidasas (y que se incluyen por lo tanto dentro del campo de la degradómica).
Esta tesis se compone de cuatro trabajos de investigación independientes en los que se caracteriza funcional y estructuralmente a distintas metalocarboxipeptidasas.
En el primer trabajo se determinó la estructura tridimensional de una isoforma corta del gen silver de Drosophila melanogaster, correspondiente al primer dominio de la carboxipeptidasa D de mamíferos. La mutación de este gen genera moscas adultas con cutículas pálidas o plateadas y con alas puntiagudas. Esta estructura tridimensional fue obtenida en presencia del inhibidor GEMSA a una resolución de 2,7 Å y se corresponde en general con la estructura canónica de otras carboxipeptidasas de su subfamilia. Presenta sin embargo como elemento distintivo un lazo flexible fácilmente accesible a las proteasas, por lo que la proteólisis podría constituir un mecanismo de regulación de esta enzima.
En el segundo trabajo se incluye la caracterización bioquímica y funcional de la carboxipeptidasa A4 humana (CPA4), la cual ha sido asociada con la agresividad del cáncer de próstata. Se determinó que esta enzima es secretada al espacio extracelular y muestra un pH óptimo neutro. Mediante aproximaciones peptidómicas se identificaron distintos péptidos bioactivos como posibles sustratos de la CPA4: la neurotensina, diversas graninas y péptidos opioides tales como la Met- o Leu- encefalina. Estos péptidos están involucrados en la proliferación y diferenciación de las células de cáncer de próstata, y permiten explicar la asociación de esta enzima con la agresividad de este tipo de cáncer. En definitiva, la CPA4 funcionaría en el procesamiento de neuropéptidos a nivel extracelular.
El tercer trabajo comprende el desarrollo de una nueva aproximación proteómica para el estudio de las preferencias de sustrato de las carboxipeptidasas. Esta técnica, basada en la tecnología COFRADIC, permite aislar miles de productos de la acción de carboxipeptidasas sobre una librería de péptidos (generada a partir de un proteoma celular). Esta técnica permitió describir las poco caracterizadas preferencias de sustrato de la carboxipeptidasa de mastocitos.
En el cuarto trabajo se aplicó una técnica proteómica denominada “COFRADIC C-terminal” que permite la búsqueda de sustratos naturales de carboxipeptidasas. En particular, esta técnica se aplicó a la búsqueda de sustratos naturales de la carboxipeptidasa citosólica 1 (CCP1), la cual estaría asociada a mecanismos de degeneración y regeneración neuronal que aún se desconocen. Se ha propuesto que CCP1 tiene como sustratos a la tubulina y a otras proteínas que presentan residuos de glutámico en su extremo C-terminal. En este trabajo se pudieron identificar 7 nuevos posibles sustratos protéicos para CCP1.<br>Proteases are enzymes that irreversibly cleave proteins by the catalysis of peptide-bond hydrolysis. With all proteins undergoing proteolysis at any point of their life cycle, proteases regulate virtually every biological process. Carboxypeptidases are proteolytic enzymes that catalyze the hydrolisis of peptidic bonds at the C-terminus of peptides and proteins. In this thesis, we studied carboxypeptidases from the M14 family (according to the MEROPS database classification), hereafter described as metallocarboxypeptidases. Metallocarboxypeptidases play key roles in controlling various biological processes, including blood coagulation/fibrinolysis, blood pressure regulation, pro-hormone and neuropeptide processing; and are also implicated in various pathological conditions such as cancer or neurodegenerative disorders.
Despite the important functions performed by these enzymes, there is usually limited knowledge about their in vivo biological roles. The present thesis was aimed to gain insights into the understanding of the biological functions of different metallocarboxypeptidases. For this purpose, we applied different approaches that included kinetic studies, cell biological studies and structural characterization. In addition, we participated in the development of different proteomic tools for protease/carboxypeptidase substrate determination (degradomics).
The present thesis consists of four independent research works that focus in the structural and functional characterization of different metallocarboxypeptidases.
The first work presents the crystal structure of a short isoform of Drosophila melanogaster silver gene, which corresponds to the first repeat of a mammalian CPD. Silver gene is responsible for the silver mutation, characterized for adult flies that display cuticles that are pale and silvery in color, and pointed wings. This three-dimensional structure was solved in presence of an inhibitor (GEMSA) at 2.7 Å resolution and overall corresponds with the structure of other members of its subfamily of metallocarboxypeptidases. A unique structural element in the here presented structure is a surface hotspot targetable by peptidases, suggesting that this enzyme might be regulated (i.e., inactivated) by proteolysis.
The second work comprises the biochemical and functional characterization of human carboxypeptidase A4 (CPA4), an enzyme that has been associated with prostate cancer aggressiveness. We found that this enzyme is secreted outside the cells and displays a neutral pH optimum that is compatible with a function in the extracellular environment. A peptidomic study identified several biologically relevant putative peptidic substrates of CPA4: neurotensin, granins, and opioid peptides such as Met- and Leu-enkephalin. These peptides are involved in the proliferation and differentiation of prostate cancer cells, potentially explaining the link between CPA4 and cancer aggressiveness. Altogether, CPA4 would function in the extracellular neuropeptide processing.
The third work comprises the development of a novel proteomic approach to study the substrate preferences of carboxypeptidases. This technology can be described as a COFRADIC-based proteome-derived peptide library approach and allows for the enrichment of thousands of carboxypeptidase products from natural, proteome-derived peptide libraries. This approach served to delineate the previously little studied substrate specificity profile of mouse mast cell carboxypeptidase.
In the fourth work we applied a proteomic tool (C-terminal COFRADIC) that allows searching for natural substrates of carboxypeptidases. Particularly, we searched for natural substrates of cytosolic carboxypeptidase 1 (CCP1) in a cellular system. This enzyme is considered to be a molecular link between neuronal degeneration and regeneration, although the molecular pathways in which CCP1 is implicated remain undefined. It has been proposed that CCP1 would posttranslationally modify tubulin and other proteins that present glutamate-stretches in their C-terminus. Here, we were able to identify seven new putative CCP1 protein substrates.
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Fernández, Ortega Carlos. "A differential spectroscopic study of a faint solar twin in the open cluster M67." Thesis, Uppsala universitet, Observationell astrofysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302601.
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A solar twin is a star with characteristics very similar to those of the Sun. This is, a Sun-like star. Earlier studies show that M67-1194 is one of the best solar twins found to date. But this star is not exactly like the Sun. There exist differences between them, in terms of chemical composition, that were defined as well. The goal of this study is to apply a new method to study the similarities of the mentioned star with the Sun. Hopefully a method that provides more precise results. The method uses difference spectra, result of subtracting the spectrum of the star from the solar spectrum, instead of the regular spectra. Then the question is: can this method cast more precise results than those obtained by Önehag et al. in 2011? The answer is that it does. The earlier study gave an uncertainty of ±0.02 dex and ours gave uncertainties between ±0.01 dex and ±0.02 dex. Nevertheless, this conclusion must be taken with caution as the study is not as conclusive as the one out carried by Önehag et al..<br>En soltvilling är en stjärna som liknar solen väldigt mycket. Tidigare studier visar att M67-1194 är en av de bästa upptäckta soltvillingarna hittills. Men stjärnan är inte exakt som solen. Det finns skillnader mellan dem angående deras kemiska sammansättningar vilket har också visats i tidigare studier. Den här studiens syfte är att tillämpa en ny metod för att granska likheterna mellan den nämnda stjärnan och solen. En metod som förhoppningsvis ger noggrannare resultat. Metoden använder differensspektra som erhålls genom att subtrahera stjärnans spektrum från solens spektrum, i stället för att använda de vanliga spektra. Frågan är om den här nya metoden kan ge noggrannare resultat än dem som Önehag et al. fick 2011. Svaret är ja. Den tidigare studien visade en osäkerhet på ±0,02 dex medan vår gav osäkerheter mellan ±0,01 dex och ±0,02 dex. Icke desto mindre måste våra slutsatser tas försiktigt ty vår studie är inte lika säker som Önehags et al..
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Martins, Bruno Leonardo Canto. "Aglomerado estelar M67: processos de dilui??o e a evolu??o do Momentum Angular." Universidade Federal do Rio Grande do Norte, 2007. http://repositorio.ufrn.br:8080/jspui/handle/123456789/16651.
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Previous issue date: 2007-08-06<br>Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior<br>Galactic stellar clusters have a great variety of physical properties that make valuable probes of stellar and galactic chemical evolution. Current studies show a discrepancy between the standard evolutionary models and observations, mainly considering the level of mixing and convective dilution of light elements, as well as to the evolution of the angular momentum. In order to better settle some of these properties, we present a detailed spectroscopic analysis of 28 evolved stars, from the turn-off to the RGB, belonging to the stellar open cluster M67. The observations were performed using UVES+FLAMES at VLT/UT2. We determined stellar parameters and metallicity from LTE analysis of Fe I and Fe II lines between 420 1100 nm. The Li abundance was obtained using the line at 6707.78 ˚A, for the whole sample of stars. The Li abundances of evolved stars of M67 present a gradual decreasing when decreasing the effective temperature. The Li dilution factor for giant stars of M67 with Teff ∼ 4350K is at least 2300 times greater than that predicted by standard theory for single field giant stars. The Li abundance as a function of rotation exhibits a good correlation for evolved stars of M67, with a much smaller dispersion than the field evolved stars. The mass and the age seem to be some of the parameters that influence this connection. We discovered a Li-rich subgiant star in M67 (S1242). It is member of a spectroscopic binary system with a high eccentricity. Its Li abundance is 2.7, the highest Li content ever measured for an evolved star in M67. Two possibilities could explain this anomalous Li content: (i) preservation of the Li at the post turn off stage due to tidal effects, or (ii) an efficient dredge-up of Li, hidden below the convective zone by atomic diffusion occurring in the post turn off stage. We also study the evolution of the angular momentum for the evolved stars in M67. The results are in agreement with previous studies dedicated to evolved stars of this cluster, where stars in the same region of the CM-diagram have quite similar rotations, but with values that indicate an extra breaking along the main sequence. Finally, we analize the distributions of the average rotational velocity and of the average Li abundance as a function of age. With relation to the average Li abundances, stars in clusters and field stars present the same type of exponencial decay law t−β. Such decay is observed for ages lesser than 2 Gyr. From this age, is observed that the average Li abundance remain constant, differently of the one observed in the rotation age
connection, where the average rotational velocity decreases slowly with age<br>Os aglomerados estelares possuem uma grande variedade de propriedades f?sicas que os tornam provas valiosas da evolu??o estelar e gal?ctica. Estudos recentes mostram uma discrep?ncia entre o modelo padr?o de evolu??o e as observa??es, principalmente com rela??o ao n?vel de mistura e dilui??o convectiva de elementos leves, bem como na evolu??o do momentum angular. Para melhor estabelecer algumas destas propriedades, apresentamos uma an?lise espectrosc?pica detalhada de 28 estrelas evolu?das, do turn off ao ramo das gigantes, pertencentes ao aglomerado estelar aberto M67. As observa??es foram feitas com o espectr?grafo UVES+FLAMES no VLT/U2. Determinamos os par?metros estelares e a metalicidade a partir de an?lises em ETL de linhas de Fe I e Fe II entre 420 1100 nm. A abund?ncia de 7Li foi obtida a partir da linha do l?tio em 6707.78 ˚A, para todas as estrelas da amostra. A abund?ncia de 7Li apresenta, para as estrelas evolu?das de M67, um gradual decrescimento na medida em que a temperatura efetiva diminui. O fator de dilui??o do Li para estrelas gigantes de M67 com Tef ∼ 4350K ? pelo menos 2300 vezes superior ao previsto pela teoria padr?o para estrelas gigantes simples de campo. A abund?ncia de Li em fun??o da rota??o apresenta uma boa correla??o para as estrelas evolu?das de M67, com uma dispers?o muito menor do que para as estrelas de campo. A massa e a idade parecem ser alguns dos par?metros que influenciam tal conex?o. Um outro resultado interessante de nosso trabalho diz respeito ? descoberta de uma estrela subgigante rica em Li de M67 (S1242). Ela ? membro de um sistema bin?rio espectrosc?pico com alta excentricidade. Sua abund?ncia de Li ? 2,7, a maior at? ent?o medida em uma estrela evolu?da de M67. Duas possibilidades podem explicar este conte?do an?malo de Li: (i) preserva??o do Li nos est?gios p?s turn off devido a efeitos de mar? gravitacional, ou (ii) uma dragagem eficiente do Li presente nas camadas abaixo da zona iii convectiva atrav?s de difus?o at?mica em est?gios p?s-turn off. Tamb?m estudamos a rela??o do 7Li com a evolu??o do momentum angular em estrelas evolu?das de M67. Os resultados encontrados est?o em acordo com estudos anteriores dedicados a estrelas evolu?das deste aglomerado, onde as estrelas de uma mesma regi?o do diagrama CM possuem rota??es bastante similares, por?m com valores que apontam para uma desacelera??o extra ao longo da seq??ncia principal. Por fim, analisamos as distribui??es da velocidade rotacional m?dia e da abund?ncia
m?dia de Li em fun??o da idade. Com rela??o a abund?ncia m?dia de Li, tanto as estrelas em aglomerados quanto as estrelas de campo, apresentam um mesmo decrescimento exponencial do tipo t−β. Tal decrescimento s? ? observado para idades menores do que 2 Giga-anos. A partir desta idade, observa-se que a abund?ncia m?dia de Li mant?m-se constante, diferentemente do que ? observado na conex?o rota??o idade, onde a velocidade rotacional m?dia decresce lentamente com a idade
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Oliveira, Gislana Pereira de. "Sobre a composi??o qu?mica em estrelas evolu?das do aglomerado aberto M67." Universidade Federal do Rio Grande do Norte, 2012. http://repositorio.ufrn.br:8080/jspui/handle/123456789/18601.
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Previous issue date: 2012-06-29<br>Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior<br>The Galaxy open clusters have a wide variety of physical properties that make
them valuable laboratories for studies of stellar and chemical evolution of the Galaxy.
In order to better settle these properties we investigate the abundances of a large number
of chemical elements in a sample of 27 evolved stars of the open cluster M67 with
different evolutionary stages (turn-off, subgiant and giant stars). For such a study we
used high-resolution spectra (R 47 000) and high S/N obtained with UVES+FLAMES at
VLT/UT2, covering the wavelength interval 4200-10 600 ?. Our spectral analysis is based
on the MARCS models of atmosphere and Turbospectrum spectroscopic tool. The oxygen
abundances were determined from the [O I] line at 6300 ?. In addition, we have also computed
abundances of Si I, Na I, Mg I, Al I, Ca I, Ti I, Co I, Ni I, Zr I, La II and Cr I. The
abundances investigated in this work, combined with their stellar parameters, offers an
opportunity to determine the level of mixing and convective dilution of evolved stars in
M67. Based on the obtained parameters, the abundances of these seem to follow a similar
trend to the curve of solar abundances. Additionally, following strategies of other studies
have investigated the relative abundances as a function of effective temperature and metallicity,
where it was possible to observe an abundance of Na, Al and Si to the stars in
the field of giants. A large dispersion from star to star, is observed in the ratios [X / Fe]
for the Co, Zr and La, and the absence of Zr and La, in the stars of the turn-off. Comparisons
made between our results and other studies in the literature show that values of
abundances are in agreement and close to the limits of the errors<br>Os aglomerados abertos da gal?xia possuem uma grande variedade de propriedades
f?sicas que os tornam valiosos laborat?rios para estudos da evolu??o qu?mica estelar
e da Gal?xia. A fim de melhor compreender essas propriedades n?s investigamos a
abund?ncia de um grande n?mero de elementos qu?micos em uma amostra de 27 estrelas
evolu?das com diferentes est?gios evolutivos (turn-off, subgigantes e gigantes) do aglomerado
aberto M67. Para tal estudo foram utilizados espectros de alta resolu??o (R 47 000)
e alto sinal ru?do obtidos com UVES+FLAMES em VLT/ UT2, cobrindo um intervalo de
comprimento de onda de 4200- 10 600 ?. Nossa an?lise espectral ? baseada nos modelos
de atmosfera MARCS e a ferramenta espectrosc?pica Turbospectrum. O oxig?nio [O I] foi
determinado a partir da linha 6300 ?. Al?m disso, tamb?m determinamos a abund?ncia
de Si I, Na I, Mg I, Al I, Ca I, Ti I, Co I, Ni I, Zr I, La II e Cr I. As abund?ncias investigadas
neste trabalho, combinadas com seus par?metros estelares, oferecem a oportunidade de
determinar o n?vel de dilui??o e mistura convectiva em estrelas evolu?das de M67. Com
base nos par?metros obtidos, as abund?ncias referidas parecem seguir uma tend?ncia semelhante
? curva de abund?ncias solares. Adicionalmente, seguindo estrat?gias de outros
estudos investigamos as abund?ncias relativas como fun??o da temperatura efetiva e da
metalicidade, onde foi poss?vel observar uma superabund?ncia de Na, Al e Si para as estrelas
no ramo das gigantes. Uma grande dispers?o, de estrela para estrela, ? observada
nas raz?es de [X/Fe] para o Co, Zr e La, al?m da inexist?ncia do Zr e La, nas estrelas do
Turn-off. Compara??es feitas entre nossos resultados e com outros estudos encontrados
na literatura mostram valores de abund?ncias que se encontram em acordo e pr?ximos
dos limites das margens de erros
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Wolfe, Sheila Pugh. "The Early Morgan Beatus (M644) : problems of its place in the Beatus pictorial tradition /." The Ohio State University, 1988. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487598303838813.
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Volek, Lukáš. "Vývojová deska s ARM Cortex M4." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2013. http://www.nusl.cz/ntk/nusl-220344.
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In this work I aimed at designing a universal system for testing either STM32F407/417 by STMicroelectronics and later various sensors and communication buses. The result then is a main board with many specific connectors for individual buses even with connectors making all I/O pins accessible at the same time. Thanks to advanced switching regulators the power supply is capable of accepting a wide range of sources like single Li-Ion cell, pair of alkaline cells, 12V Lead battery, common wall power adapters (both DC and AC up to 15 Vpp ), USB, laboratory power supplies with multiple outputs and finally POE (Power Over Ethernet). Supply voltages are supervised by voltage comparators with an optical signalisation. (It is possible to determine the sick branch without a measuring instrument and blowing components in the most cases.) Another important parameter was a robustness of the supply and communication lines. There is a number of TVSs, chokes, and big and low ESR capacitors A PC software is intended for a basic functionality demonstration only.
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Alfaro, Cuello Mayte [Verfasser], and Nadine [Akademischer Betreuer] Neumayer. "The Nucleus of the Sagittarius Dwarf Spheroidal Galaxy: M54 / Mayte Alfaro Cuello ; Betreuer: Nadine Neumayer." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1196207690/34.
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Baluchova´, Katari´na. "In vitro and in vivo studies of murine cytomegalovirus mutated in M34 and M35 ORFs." Thesis, University of Birmingham, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408937.
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Cytomegalovirus is an important human pathogen causing life-threatening and debilitating disorders in some immunocompromised individuals. This double-stranded DNA betaherpesvirus is one of the largest and most complex viruses which establishes latency in the host. Treatment available for symptomatic patients is limited and development of new antiviral strategies is highly desired. Understanding of the virulence and pathogenesis of HCMV requires functional analysis of at least 164 gene products. Due to the species-specificity of HCMV and its inability to replicate in animals, functional analysis of HCMV encoded gene products relies on studies of animal CMVs in their natural hosts. Murine CMV (MCMV) shares a high degree of sequence homology with HCMV and has a similar biology in causing acute and latent infection and disease in mice. Analysis of gene function became more practical with the availability of MCMV cloned into a bacterial artificial chromosome (BAC) plasmid. Phenotypic characterisation of recombinant viruses disrupted in the M34 or M35 ORF, the homologues of HCMV UL34 and UL35 ORF respectively, is presented here. Infectious viruses reconstructed from the mutated BAC plasmids, the mM34 and mM35, had the expected genome rearrangements as indicted by restriction enzyme analysis, PCR and partial sequencing. In vitro, mM34 and mM35 viruses were attenuated in their replication when inoculated at a low and a high multiplicity of infection when compared to the parental virus. Similarly, these viruses were severely restricted in their replication in immunodeficient SCID mice and did not kill mice up to 28 days post-inoculation. Comparison of the predicted M34 and M35 gene products with related betaherpesviruses suggests that the M34 protein plays a role in transcriptional regulation of viral replication and the M35 protein is a component of the tegument.
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Dupont, Laurence. "Organisation, expression et évolution des gènes du bactériophage tempéré MV4 de Lactobacillus delbrueckii subsp. Bulgaricus." Toulouse 3, 1994. http://www.theses.fr/1994TOU30071.
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Lactobacillus delbrueckii subsp. Bulgaricus est une bacterie lactique largement utilisee dans l'industrie laitiere. Le bon deroulement des fermentations realisees par cette espece est souvent entrave par le developpement de bacteriophages. Nous avons choisi d'etudier le phage tempere mv4, representatif d'une grande famille de phages de lactobacillus delbrueckii subsp. Bulgaricus et lactis, pour ameliorer la resistance aux phages, mais egalement pour mieux comprendre la genetique de l'espece bacterienne sur laquelle il se propage. Une quinzaine de genes ont ete identifies grace au sequencage d'un tiers du genome phagique (12kb). Deux operons tardifs ont ete plus precisement etudies: le gene implique dans la lyse bacterienne et le gene codant la proteine majeure de la capside (34 kda). L'etude de l'expression des genes, abordee par cartographie des arn messagers, a permis de mettre en evidence une sequence consensus originale qui pourrait etre celle d'un promoteur de type tardif. De plus, un fragment d'adn phagique doue d'une activite promotrice dans un hote heterologue, lactobacillus casei, a ete caracterise. Dans une bacterie lysogene, l'adn genomique du phage mv4 est integre sous forme de prophage dans le chromosome, mais est egalement present sous forme lineaire extra-chromosomique. Pour apprehender cette forme de lysogenie originale, la region impliquee dans l'integration phagique a ete analysee: le phage mv4 integre son adn par un mecanisme de recombinaison site-specifique qui fait intervenir une enzyme codee par le phage, l'integrase, et un site d'attachement phagique attp. Cette integration a lieu dans l'extremite 3' du gene d'un arn de transfert present sur le chromosome de l'hote. La comparaison des sequences nucleotidiques du phage mv4 avec celle du phage virulent ll-h, apparente a mv4, a permis d'aborder l'evolution des genes au sein d'une meme famille de phages et de demontrer le role des phages temperes dans l'emergence de phages virulents
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Alfaro-Cuello, Mayte [Verfasser], and Nadine [Akademischer Betreuer] Neumayer. "The Nucleus of the Sagittarius Dwarf Spheroidal Galaxy: M54 / Mayte Alfaro Cuello ; Betreuer: Nadine Neumayer." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1196207690/34.
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Hixon, Lee Franklin. "Potential and Quantification of Street Sweeping Pollutant Reductions towards addressing TMDL WLAs for MS4 Compliance." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/100955.
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Municipal separate storm sewer system (MS4) permittees face costly obligations to reduce pollutant loadings needed to achieve waste load allocations (WLAs) and meet total maximum daily loads (TMDLs). Street sweeping is potentially an effective BMP since streets exist throughout urban watersheds, often are directly connected to the storm sewer, and are found to contain an abundance of contaminants. Although pollutant removal from street sweeping has been evaluated for decades, an understanding of the impact on water quality in receiving streams is elusive. Due to numerous variables, the large number of samples necessary to measure impact in receiving streams may never be obtained. In response, modeled pollutant removal efficiencies based on frequency of sweeping have been recommended to the Chesapeake Bay Program, but these results are suspect. Alternatively, the amount of swept material has emerged as a method to quantify reductions.
A sampling study was conducted to measure pollutants in swept material. The study identified the fraction of material susceptible to transport in runoff based on timing of sweeping in relation to runoff events. Based on observed pollutant concentration associations with particle size, the study results in estimates of pollutant concentrations for the fraction of material susceptible to downstream transport, dependent on duration since the last rainfall and type of surface swept, whether the area is a streets or a parking lot. Pollutant loadings and required reductions to achieve the Chesapeake Bay WLAs for various land use sample areas are computed for an average year. Modeled removal efficiencies and results from the sampling study were employed to assess impacts from street sweeping. Modeled efficiencies predict significantly lower impact than measurements of pollutants susceptible to runoff in swept material. Modeled loadings are inconsistent with measurements of swept materials and the rigorous sweeping frequency required for modeled removal efficiency credit appears to be unnecessary.<br>Doctor of Philosophy
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Morgenstern, Robert M. "Vector quantization applied to speech coding in the wireless environment." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07292009-090440/.
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