Academic literature on the topic 'Máster Classes'

In addition to cell membrane phospholipids, Actinobacteria in the order Corynebacteriales possess a waxy cell envelope containing mycolic acids (MA). In optimized culture condition, some species can also accumulate high concentrations of intracellular triacylglycerols (TAG), which are a potential source of biodiesel. Bacterial lipid classes and composition alter in response to environmental stresses, including nutrient availability, thus understanding carbon flow into different lipid classes is important when optimizing TAG synthesis. Quantitative and qualitative analysis of lipid classes normally requires combinations of different extraction, derivatization, chromatographic and detection methods. In this study, a single-step thin-layer chromatography-flame ionization detection (TLC-FID) technique was applied to quantify lipid classes in six sub-Antarctic Corynebacteriales strains identified as Rhodococcus and Williamsia species. A hexane:diethyl-ether:acetic acid solvent system separated the total cellular lipids extracted from cells lysed by bead beating, which released more bound and unbound MA than sonication. Typical profiles included a major broad non-polar lipid peak, TAG and phospholipids, although trehalose dimycolates, when present, co-eluted with phospholipids. Ultra-performance liquid chromatography-tandem mass-spectrometry and nuclear magnetic resonance spectroscopy detected MA signatures in the non-polar lipid peak and indicated that these lipids were likely bound, at least in part, to sugars from cell wall arabinogalactan. Waxy esters were not detected. The single-solvent TLC-FID procedure provides a useful platform for the quantitation and preliminary screening of cellular lipid classes when testing the impacts of growth conditions on TAG synthesis.

ABSTRACT Cryoelectron micrographs of purified human foamy virus (HFV) and feline foamy virus (FFV) particles revealed distinct radial arrangements of Gag proteins. The capsids were surrounded by an internal Gag layer that in turn was surrounded by, and separated from, the viral membrane. The width of this layer was about 8 nm for HFV and 3.8 nm for FFV. This difference in width is assumed to reflect the different sizes of the HFV and FFV MA domains: the HFV MA domain is about 130 residues longer than that of FFV. The distances between the MA layer and the edge of the capsid were identical in different particle classes. In contrast, only particles with a distended envelope displayed an invariant, close spacing between the MA layer and the Env membrane which was absent in the majority of particles. This indicates a specific interaction between MA and Env at an unknown step of morphogenesis. This observation was supported by surface plasmon resonance studies. The purified N-terminal domain of FFV Gag specifically interacted with synthetic peptides and a defined protein domain derived from the N-terminal Env leader protein. The specificity of this interaction was demonstrated by using peptides varying in the conserved Trp residues that are known to be required for HFV budding. The interaction with Gag required residues within the novel virion-associated FFV Env leader protein of about 16.5 kDa.

Penelitian ini bertujuan untuk (1) mengembangkan perangkat pembelajaran kalkulus MA yang meliputi silabus, RPP, LKS, dan instrumen penilaian untuk mencapai ketuntasan belajar dan keman-dirian belajar; (2) mendeskripsikan kualitas hasil pengembangan perangkat pembelajaran kalkulus MA untuk mencapai ketuntasan belajar dan kemandirian belajar. Penelitian ini merupakan penelitian pengembangan yang mengembangkan perangkat pembelajaran kalkulus dengan menggunakan model pengembangan yang diadaptasi dari model pengembangan Thiagarajan, Semmel, dan Semmel. Tahap-tahap yang dilalui sampai diperoleh perangkat pembelajaran kalkulus yang valid, praktis, dan efektif meliputi: (1) tahap pendefinisian, (2) tahap perencanaan, dan (3) tahap pengembangan. Uji coba yang dilakukan meliputi uji coba ahli/validasi ahli, uji coba terbatas, dan uji coba lapangan. Uji coba lapangan dilakukan di MA PK Ma’arif 01 Kebumen pada dua kelas XI IPS. Penelitian ini menghasil-kan perangkat pembelajaran kalkulus untuk MA kelas XI IPS terdiri atas silabus, RPP, LKS, dan THB yang berkualitas dan layak digunakan dalam proses pembelajaran. Masing-masing komponen perangkat pembelajaran yang terdiri atas silabus, RPP, LKS, dan THB telah memenuhi kriteria valid, praktis, dan efektif untuk mencapai ketuntasan dan kemandirian belajar siswa. Kata kunci: pengembangan, perangkat pembelajaran, kalkukus, ketuntasan belajar, kemandirian belajar. DEVELOPING A CALCULUS TEACHING PACKAGE TO ACHIEVE MASTERY AND SELF-REGULATED LEARNING Abstract This research aims to: (1) develop a calculus teaching package consisting of syllabus, lesson plan, worksheet, and test of Islamic high school to achieve mastery and self-regulated learning; (2) describe the quality of the calculus teaching in Islamic high school to achieve mastery and self-regulated learning. This research is a developmental research which develops calculus teaching package using the development model adapted from Thiagarajan, Semmel, and Semmel. The steps to get valid, practical, and effective calculus teaching package are: (1) definition step, (2) planning step, and (3) development step. The try out consisted of expert judgment, small group try out, and field try out. The field try out was held in MA PK Ma’arif 01 Kebumen at two classes of XI IPS. The research produces a calculus teaching package for class XI IPS students of MA consisting of the syllabus, lesson plan, worksheet, and test which are qualified and suitable for the teaching and learning process. Each teaching package component is valid, practical, and effective to achieve students’mastery learning and self-regulated learning. Keywords: development, teaching package, calculus, mastery learning, self-regulated learning

Melamine molecularly imprinted polymeric microsphere (MIPM) was initially prepared by two-step seed swelling polymerization methods. Factors affecting the particle morphologies including emulsifier, dispersant, the water-oil ratio, and etc were optimized in order to obtain the MIPMs with a final narrow particle diameter of 3-5 μm. Characterization of the resulted polymeric microspheres was investigated by scanning electron microscope (SEM), infrared absorption spectroscopy (IR), and mercury analyzer (MA), respectively. When the initial concentration of melamine was 1.0 mmol L-1, the affinity capacity of MIPMs was 2.0 times than that of NIPMs. Scatchard analysis suggested melamine were recognized by the prepared MIPMs with two classes of binding sites. The apparent maximum binding capacity and dissociation constant were calculated to be 43.2 μmol g-1 (Qmax1) and 2.2×10-4 mol L-1 (Kd1) for high affinity site, whilst to be 102.3 μmol g-1 (Qmax2) and 1.3×10-3 mol L-1 (Kd2) for low affinity site, respectively. Obvious molecular effects towards melamine were further confirmed by the comparative HPLC experiments using NIPMs and MIPMs as the stationary phases, respectively.

Abstract Abstract 3133 The treatment of multiple myeloma (MM) has been rapidly evolving with the introduction of new drugs, but this disease is still regarded as incurable, with few exceptions. The role of allogeneic stem cell transplantation in the treatment of MM is unclear at present. It offers the possibility to harness the curative potential of the graft-versus-myeloma effect, but high transplant-related mortality, as previously reported, has limited its use. We report our single-center experience of 120 patients treated with allogeneic transplantation at the Helsinki University Central Hospital since the year 2000 showing low transplant-related mortality and a significant proportion of patients achieving prolonged progression-free survival. The median age of the patients was 50 (range 28–65) years. Sixty-three patients were male, 57 female. The immunoglobulin classes were IgG 75, IgA 16, IgD 3, and light chain 26. Forty-nine patients received a myeloablative (MA) conditioning, 71 patients a reduced intensity conditioning (RIC). The MA regimens were cyclophosphamide 120 mg/kg + fractionated TBI 12 Gy, lungs 10 Gy (n= 30) and treosulfan 42 g/m2 + fludarabine 150 mg/m2 (n= 19), the RIC regimens TBI 2 GY (n= 52) or treosulfan 36 g/m2 (n= 19) with fludarabine. In 43 cases allotransplantation, conditioned with low-dose TBI-based regimen, was performed in a preplanned combination with autologous transplantation which preceded the allotransplantation with the median of 5 (range 2–8) months. Overall, 79 patients had an autologous transplantation in their previous history. The number of chemotherapy lines preceding allogeneic transplantation was 1 in 49, 2 in 30, and 3–7 in 41 cases. The median time from the diagnosis of MM to allotransplantation was 10 (range 5–82 ) months in transplantations with MA conditioning and 16 (7–170) months in those with RIC. Of the donors 74 were HLA-matched siblings and 46 unrelated. The graft was from peripheral blood in 99 and from bone marrow in 21 cases. In transplantations with low-dose TBI based conditioning the GVHD prophylaxis consisted of CsA and MMF. The other patients received CsA + MTX, in sibling transplantations with TBI-based MA conditioning added with methylprednisolone. ATG was given in transplantations from an unrelated donor except to patients conditioned with low-dose TBI. The state of the disease preceding the allotransplantation was the following: CR 18 (15 %), VGPR 7 (6 %) PR 82 (68 %), MR 3 (3 %), and progression 10 (8 %). At 100 days post-transplantation 30 patients (25 %) were in CR, 6 (5 %) in VGPR, 63 (53 %) in PR, 2 (2 %) in MR; 2 (2 %) had no change, and 13 (11 %) progressed. Five patients had died. The best response, achieved at the median of 3 (range 0 – 80) months post-transplantation, was CR 53 (44 %), VGPR 6 (5 %), PR 48 (40 %), MR 1 (1 %), NC 2 (2 %), and progression 10 (8 %). The median follow-up of living patients was 62 (range 1–140) months. The cumulative incidence of grade II-IV acute GVHD was 29 %; that of chronic GVHD 65 % (extensive 25 %) in MA transplantations and 80 % (extensive 51 %) in RIC transplantations. Seventy-three patients had disease progression; the cumulative incidence of progression until 10 years was 70%. There was no significant difference in the incidence of progression between MA and RIC transplantations. Thirty-six patients received DLI for the treatment of progression. Fifty-three patients have died, 38 of them in disease progression, and 15 of non-relapse causes. The cumulative incidences of non-relapse mortality (NRM) in MA and RIC transplantations were 0 vs. 3 % at 100 days, 2 vs. 9 % at 1 year and 8 vs. 26 % at 5 years. The survivals of MA and RIC patients were 92 vs. 87% at 1 year, 87 vs. 72% at 2 years, 64 vs. 56% at 5 years, and 49 vs. 35% at 10 years, respectively. The proportion of the whole patient population surviving without disease progression was 26% from 7 years on until the end of follow-up. There was no significant difference in the survival between transplantations from sibling and unrelated donor. In conclusion, the low NRM, particularly in transplantations with myeloablative conditioning, and the significant proportion of patients with long-term progression-free survival suggest a role for allogeneic transplantation in the treatment of myeloma. The optimal combination of modern drug treatment with allogeneic transplantation providing graft-versus myeloma effect should be assessed in clinical trials. Disclosures: No relevant conflicts of interest to declare.

The aromatase enzyme catalyses the last step in estrogen biosynthesis. There are two classes of third-generation aromatase inhibitors: irreversible steroidal inhibitors (e.g. exemestane) and reversible non-steroidal inhibitors (e.g. anastrozole, letrozole). All three agents have been found to be equivalent or superior to megestrol acetate as second-line therapy for metastatic breast cancer. In the first-line setting, large phase III trials have shown that all three are equivalent or superior to tamoxifen in women with metastatic disease. Several large trials with varying study designs have been launched to analyse their role in the adjuvant setting. The four that have reported found longer average disease-free survival for women who received an aromatase inhibitor than for those who did not. In addition, one trial, MA.17, has shown a survival advantage associated with the use of an aromatase inhibitor in node-positive patients. Guidelines produced by the American Society of Clinical Oncology suggest that adjuvant therapy for postmenopausal women with hormone-receptor-positive breast cancer should include an aromatase inhibitor. However, the long-term consequences of estrogen deprivation in postmenopausal women remain uncertain, particularly with regard to bone and cardiovascular health. The advantages and disadvantages of different hormonal strategies should therefore be carefully considered in each individual case.

Abstract Pediatric AML still represent an unfavourable disease resulting from the heterogeneous clonal expansion of malignant transformed haematopoietic stem or progenitor cell. The leukemia cell population is continuously replenish by rare, functionally distinct “leukaemia stem cells” (LSC) endowed with the capacity to self renew as well with the ability to generate clonogenic leukemic progenitors The AML-LSCs have been well documented and seem to behave like quiescent or slowly dividing hematopoietic stem cells. Therefore, LSC are considered less sensitive to treatments, which rather target actively dividing cells, and responsible for relapse. Recently, Y. Ma et al. suggested a major role of SALL4 gene both in stemness activity and leukemia transformation of normal hematopoietic stem cells. We sought to evaluate the expression of SALL4 gene in a panel of 88 pediatric AML, 60 Acute Lymphoblastic Leukemia (T and B ALL) and a few hematopoietic normal tissues. SALL4 expression was determined by quantitative RT-PCR in pre-treatment bone marrow samples (BM) (median blasts: 80%) and in normal tissues. SALL4 expression was much higher in AML compared to ALL (p<0.0001) and normal tissue (p < 0.0001). Values varied greatly among AML samples (range: 0 to 144; median 0.3). AML samples were dichotomized at SALL4’s median expression into“low” (n=44) or “high“(n=44) expressers. High expression was correlated to cytogenetic risk, as defined by MRC classes (p= 0.0008) but not to FAB categories, WBC, MLL rearrangements or FLT3, N RAS and NPM1 genotypes. Low expression was significant associated to t(8;21) translocation (P=0.02). SALL expression progressively increased from MRC1 class (low risk) to MRC3 (high risk) (p=0.002). High SALL4 patients had a shorter OS (OS: 46.8%±8% vs 74%±7%, p= 0.035) and a shorter EFS (EFS: 40% ± 8% vs 58% ±8%; P= 0.2) compared to low SALL4 expressers, although the latter association was less strong. As the highest values of SALL4 correlated with intermediate (MRC2) and high (MRC3) cytogenetic risk, we looked at fitting a model based upon the 4 quartiles of SALL4 expression. The upper quartile (values > 16, n= 12) had the worst outcome compared to the three others. Once stratified on MRC groups, MRC2 patients in the upper SALL4 quartile had 3.2 times more risk of relapse (HR= 3.2, CI95%: 1.3–7.8, P=0.02) and 5.4 more risk to die (HR=5.4, CI95%: 1.8–7.6; P= 0.0005) than MRC2 patients in the three others quartiles. In conclusion, SALL4 expression level may define an important risk factor in AML, particularly among patients with cytogenetic intermediate risk.

This study aimed to explore the perceived barriers to physical activity among college students Study Design: Qualitative research design Eight focus group discussions on 67 college students aged 18-24 years (48 females, 19 males) was conducted on College premises. Data were analysed using inductive approach. Participants identified a number of obstacles to physical activity. Perceived barriers emerged from the analysis of the data addressed the different dimensions of the socio-ecological framework. The result indicated that the young adults perceived substantial amount of personal, social and environmental factors as barriers such as time constraint, tiredness, stress, family control, safety issues and much more. Understanding the barriers and overcoming the barriers at this stage will be valuable. Health professionals and researchers can use this information to design and implement interventions, strategies and policies to promote the participation in physical activity. This further can help the students to deal with those barriers and can help to instil the habit of regular physical activity in the later adult years.

Diameter at breast height (DBH) distributions offer valuable information for operational and strategic forest management decisions. We predicted DBH distributions using Norwegian national forest inventory and airborne laser scanning data and compared the predictive performances of linear mixed- effects (PPM), generalized linear-mixed (GLM) and k nearest neighbor (NN) models. While GLM resulted in smaller prediction errors than PPM, both were clearly outperformed by NN. We therefore studied the ability of the NN model to improve the precision of stem frequency estimates by DBH classes in the 8.7 Mha study area using a model-assisted (MA) estimator suitable for systematic sampling. MA estimates yielded greater than or approximately equal efficiencies as direct estimates using field data only. The relative efficiencies (REs) associated with the MA estimates ranged between 0.95–1.47 and 0.96–1.67 for 2 and 6 cm DBH class widths, respectively, when dominant tree species were assumed to be known. The use of a predicted tree species map, instead of the observed information, decreased the REs by up to 10%.

Abstract. Hossen S, Hossain MK, Hossain MA, Uddin MF. 2020. Quantitative assessment of tree species diversity of Himchari National Park (HNP) in Cox’s Bazar, Bangladesh. Asian J For 5: 1-7. The aim of the study was to assess the tree species composition, dominance, and quantitative distribution of tree species of Himchari National Park, Cox’s Bazar in Bangladesh through stratified random sampling method using sample plots (51) of 20 m x 20 m in size during the period of January 2017 to May 2018. A total of 961 stems (dbh ≥ 5 cm) of 88 tree species belonging to 64 genera and 37 families were enumerated where the stem density and basal area were 457.39 stem ha-1 and 10.979 m2 ha-1 respectively. On the other hand, the species diversity index, Shannon-Wiener’s diversity index, Shannon’s maximum diversity index, species evenness index, Margalef’s diversity index, and Simpson’s diversity index were 0.092, 3.733 ± 0.0071, 4.477, 0.834, 12.667 and 0.039 ± 0.0003 respectively. The highest Importance Value Index (IVI) was found for Acacia auriculiformis (23.23) followed by Tectona grandis (13.05), Gmelina arborea (12.66), Syzygium fruticosum (12.34), Casuarina equisetifolia (10.57), and Dipterocarpus turbinatus (10.55). The IVI value represents that Acacia auriculiformis possess highest dominance that is followed by Tectona grandis and Gmelina arborea. Percentage distribution of tree individuals into different height classes found in quadrats showed that height range 3 - <8 m had the highest (59.83%) percentage of tree individuals. On the other hand, different dbh (having dbh ≥5 cm) classes showed that most of the trees (65.97%) belonged to dbh range 5 - <15 cm. The outcome of present study suggests for the protection, sustainable management, and conservation of the tree resources of HNP, Cox’s Bazar, Bangladesh.