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1

HOLM, SØREN. "The Bioethicist Who Cried “Synthetic Biology”: An Analysis of the Function of Bioterrorism Predictions in Bioethics." Cambridge Quarterly of Healthcare Ethics 26, no. 2 (March 31, 2017): 230–38. http://dx.doi.org/10.1017/s0963180116000827.

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Abstract:This article analyzes a specter that has haunted bioethics almost since its inception, namely the specter of the misuse of biotechnology by maleficent agents bent on mass destruction, or the complete eradication of human kind and life as we know it. The article provides a general account of why bioethicists cry “catastrophic bioterrorism potential” when new biotechnologies emerge, and an analysis of the arguments that flow from the prediction, especially in relation to synthetic biology.
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2

Jenkins, N. "Mass spectrometry for biotechnology." Biochemical Education 27, no. 1 (January 1999): 64. http://dx.doi.org/10.1016/s0307-4412(98)00242-8.

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3

Mladenovic-Drinic, Snezana, Dragana Ignjatovic-Micic, Iva Eric, Violeta Andjelkovic, Drazen Jelovac, and Kosana Konstantinov. "Biotechnology in maize breeding." Genetika 36, no. 2 (2004): 93–109. http://dx.doi.org/10.2298/gensr0402093m.

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Maize is one of the most important economic crops and the best studied and most tractable genetic system among monocots. The development of biotechnology has led to a great increase in our knowledge of maize genetics and understanding of the structure and behaviour of maize genomes. Conventional breeding practices can now be complemented by a number of new and powerful techniques. Some of these often referred to as molecular methods, enable scientists to see the layout of the entire genome of any organism and to select plants with preferred characteristics by "reading" at the molecular level, saving precious time and resources. DNA markers have provided valuable tools in various analyses ranging from phylogenetic analysis to the positional cloning of genes. Application of molecular markers for genetic studies of maize include: assessment of genetic variability and characterization of germ plasm, identification and fingerprinting of genotypes, estimation of genetic distance, detection of monogamic and quantitative trait loci, marker assisted selection, identification of sequence of useful candidate genes, etc. The development of high-density molecular maps which has been facilitated by PCR-based markers, have made the mapping and tagging of almost any trait possible and serve as bases for marker assisted selection. Sequencing of maize genomes would help to elucidate gene function, gene regulation and their expression. Modern biotechnology also includes an array of tools for introducing or deieting a particular gene or genes to produce plants with novel traits. Development of informatics and biotechnology are resulted in bioinformatic as well as in expansion of microarrey technique. Modern biotechnologies could complement and improve the efficiency of traditional selection and breeding techniques to enhance agricultural productivity.
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Jittayasothorn, Yingyos, Jiang Lu, Xia Xu, Piyada Thipyapong, and Nantakorn Boonkerd. "(284) Factors Influencing Somatic Embryogenesis of Proembryonic Mass Suspension Culture in `Autumn Royal Seedless' Grape." HortScience 41, no. 4 (July 2006): 1053B—1053. http://dx.doi.org/10.21273/hortsci.41.4.1053b.

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Biotechnology has great potential for grape genetic improvement. However, successful implementations of grape biotechnologies, such as transformation and in-vitro selection, are based on a high yield productivity of synchronized somatic embryos as well as an efficient single-cell regeneration system. Suspension culture has been known as an ultimate approach to provide those requirements. We recently developed the highly repeatable protocol for PEM suspension culture of `Autumn Royal Seedless' (Vitis vinifera L.). In a following experiment, three factors, including activated charcoal (AC), darkness, and full- or half-strength MS medium were tested for their impact on grape embryogenesis of PEM suspension cells. All three factors proved to be important for grape somatic embryogenesis. Darkness was the most-influenced factor among the three. After 4 weeks on full-strength MS medium plus AC, suspension cells mostly grew friable callus and somatic embryos were rarely observed under 16-h light conditions, whereas numerous somatic embryos were fully developed in darkness. Strength of MS medium and AC also affected grape somatic embyogenesis. In every combination tested, full-strength MS media was all superior to half-strength, which was more obvious under darkness. The AC had a positive effect for promoting of somatic embryogenesis.
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Mini, Silvio. "Genetics and biotechnologies in Italian mass media." Journal of Science Communication 04, no. 03 (September 21, 2005): A03. http://dx.doi.org/10.22323/2.04030203.

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Several researchers operating in the sociological field have recently theorised that genetics and biotechnologies are at the core of the public perception of science. The present study aims at verifying empirically whether or not this is mirrored in Italian mass media, as well as at analysing the topics most frequently present in Italian newspapers and the economic and editorial reasons behind the results of editorial choices. Besides, it provides statistics about the major Italian newspapers published in the last third of 2002. This period has been chosen because some important news was published in December: it consequently offered the chance to carry out a long-term analysis as well as a study of the most important differences - in content and editorial lay-out - between scientific articles which are published in the appropriate sections inside the newspaper and those which make the front page. Ours are going to be purely quantitative considerations; but, from the point of view of the content, the data are sufficient to identify various narrative currents. These currents could be the object of further research on the frames used to contextualize the news and the reasons (anthropological, socio-cultural and editorial) for the way they are used by editorial staffs.
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6

Ifezouane, Jihane, Fadoua Berdi, Soufiane El Marrakchi, and Jamal Lamsaouri. "Medicines derived from biotechnology / Biosimilars in Morocco: economic and regulatory aspect." Batna Journal of Medical Sciences (BJMS) 7, no. 1 (May 2, 2020): 10–14. http://dx.doi.org/10.48087/bjmsra.2020.7103.

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Les biomédicaments permettent un meilleur accès au traitement spécialement pour les affections de longue durée (cancer, diabète, maladie de sang,…) leur marché représente un enjeu important pour l’industrie pharmaceutique, Les brevets de certains biomedicaments arrivent déjà à échéance, ouvrant la voie à d’autres fabricants pour mettre sur le marché des produits dits biosimilaires qui sont moins chers que les biomedicaments de référence et ils peuvent ainsi représenter une source d’économie importante pour les système de santé. Les processus de développement et de fabrication de ces biosimilaires sont plus complexes et plus coûteux que ceux des génériques de médicaments chimiques et expliquent en dernier lieu les grandes différences de prix entre ces deux types de médicaments. Les biosimilaires ne peuvent pas être exactement identiques aux biomédicaments de référence mais ils ont un profil de qualité, d’efficacité et de sécurité similaire à celui–ci, et la démonstration de la similarité nécessite de nouveaux essais précliniques et cliniques. Au Maroc, un nouveau décret vient poser un cadre réglementaire garantissant l'autorisation de produits comparables à la référence, sûrs et efficaces Toutefois, la question de l’interchangeabilité reste posée dans la mesure où ces produits présentent des variabilités de l’un à l’autre.
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7

Marks, Leonie A., Nicholas Kalaitzandonakes, Lee Wilkins, and Ludmila Zakharova. "Mass media framing of biotechnology news." Public Understanding of Science 16, no. 2 (March 20, 2007): 183–203. http://dx.doi.org/10.1177/0963662506065054.

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8

Thudium, Karen, Sanela Bilic, Douglas Leipold, William Mallet, Surinder Kaur, Bernd Meibohm, Hans Erickson, Jay Tibbitts, Hong Zhao, and Manish Gupta. "American Association of Pharmaceutical Scientists National Biotechnology Conference Short Course: Translational Challenges in Developing Antibody-Drug Conjugates." mAbs 5, no. 1 (January 2013): 5–12. http://dx.doi.org/10.4161/mabs.22909.

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9

Carr, Steven A., Mark E. Hemling, Mark F. Bean, and Gerald D. Roberts. "Integration of mass spectrometry in analytical biotechnology." Analytical Chemistry 63, no. 24 (December 15, 1991): 2802–24. http://dx.doi.org/10.1021/ac00024a003.

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10

Nguyen, D. N., G. W. Becker, and R. M. Riggin. "Protein mass spectrometry: applications to analytical biotechnology." Journal of Chromatography A 705, no. 1 (June 1995): 21–45. http://dx.doi.org/10.1016/0021-9673(94)01256-e.

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11

Boy, Daniel, and Flora Chanvril. "Les représentations sociales des technologies du vivant en Europe." Sociologie et sociétés 42, no. 2 (January 20, 2011): 17–42. http://dx.doi.org/10.7202/045354ar.

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Depuis une vingtaine d’années de multiples controverses publiques se sont développées à propos de l’usage des biotechnologies. En matière de biotechnologies « vertes », c’est-à-dire principalement liées à la fabrication de plantes transgéniques, l’accent a été mis sur les risques encourus par les consommateurs et l’environnement, et l’on a souvent souligné le défaut de bénéfice immédiat pour les consommateurs. En ce qui concerne les biotechnologies « rouges », c’est-à-dire pour l’essentiel celles qui concernent le domaine médical, la notion de risque est sans doute plus ambiguë puisque les bénéfices attendus pour les patients paraissent plus directs. Mais au-delà du calcul risque-bénéfice, les biotechnologies « rouges » ont des conséquences possibles dans le domaine de l’éthique qui sont apparues pour la première fois de façon éclatante au moment du clonage de la brebis Dolly. L’analyse d’enquêtes par sondage réalisées au sein des pays de l’Union Européenne (Eurobaromètres) permet d’explorer l’univers des représentations sociales des Européens dans ce domaine.
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12

Goodacre, Royston, and Douglas B. Kell. "Pyrolysis mass spectrometry and its applications in biotechnology." Current Opinion in Biotechnology 7, no. 1 (February 1996): 20–28. http://dx.doi.org/10.1016/s0958-1669(96)80090-5.

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13

Aebersold, Ruedi. "Mass spectrometry of proteins and peptides in biotechnology." Current Opinion in Biotechnology 4, no. 4 (August 1993): 412–19. http://dx.doi.org/10.1016/0958-1669(93)90006-i.

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14

Kumar, Avinash, and Sambit Mallick. "Marker-Assisted Selection (MAS) in Rice Biotechnology Research in India." Perspectives on Global Development and Technology 18, no. 3 (May 17, 2019): 286–307. http://dx.doi.org/10.1163/15691497-12341520.

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Abstract The present study examines the potential of Marker-Assisted Selection (MAS) technology in the field of rice biotechnology research in India and how it is perceived and practiced by rice biotechnologists in selected scientific institutions under the aegis of Indian Council of Agricultural Research (ICAR) and State Agricultural Universities (SAUs). The primary data for the study has been collected through in-depth personal interviews with molecular biologists and plant breeders engaged in rice biotechnology research in various institutional settings. A majority of the scientists emphasize the relative merits of MAS vis-à-vis the currently contentious transgenic approach for rice improvement in India. The MAS has emerged as an integrative, non-evasive, non-controversial, non-proprietary, and benign technology with the wider social acceptance that promotes open-mode, inclusive, and user-centered innovations in rice biotechnology research.
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DUBOIS, Lise. "L’aliment, un futur miracle de la biotechnologie?" Sociologie et sociétés 28, no. 2 (September 30, 2002): 45–57. http://dx.doi.org/10.7202/001203ar.

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Résumé L'aliment entre dans une ère nouvelle de transformations biotechnologiques qui lui permettra de devenir un moyen d'intervention sur le corps au niveau génétique, ce qui soulève des questions d'ordre éthique. La science établit tics liens de plus en plus nombreux entre l'alimentation et la santé qui permettent à l'agro-alimentaire de développer des produits d'un nouveau genre : l'aliment fonctionnel, qui se situe entre l'aliment et le médicament. Ultimement, il sera possible d'offrir au consommateur les aliments correspondants à ses besoins spécifiques. Mais d'ici là, il faut s'interroger sur l'innocuité des produits et leur efficacité réelle. Ces produits santé qui constituent un marché à venir important pour l'agro-alimentaire sont de plus en plus difficiles à réglementer, et on doit s'assurer qu'ils ne contribueront pas à accroître les inégalités.
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16

Moser, Antônio. "Apenas questão de gênero?" Revista Eclesiástica Brasileira 76, no. 301 (August 10, 2018): 44–74. http://dx.doi.org/10.29386/reb.v76i301.233.

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Síntese: As discussões sobre gênero se encontram mais acesas do que nunca. Por isso mesmo, merecem atenção. Para uma abordagem equilibrada convém partir dos mistérios do corpo e da sexualidade. Apesar das aparências em contrário, corpo e sexualidade são realidades dinâmicas, sujeitas a mudanças mais ou menos constantes. As biotecnologias acentuam ainda mais essa possibilidade de mudanças até há pouco, ou espontâneas ou efetivadas através de meios mais ou menos convencionais. Por isso mesmo, não podem ser ignoradas. A questão mais importante parece apontar para um diálogo sereno, através do qual se consiga mergulhar um pouco mais profundamente na condição humana. Toda radicalização tende a distorcer a realidade. A busca de um equilíbrio é fundamental neste momento em que a tentação dos reducionismos se faz presente em todas as realidades humanas. E o equilíbrio é encontrado na medida em que se valoriza tanto o esse, quanto o fieri: o que muda e o que permanece.Palavras-chave: Gênero. Corpo. Sexualidade. Biotecnologias. Alterações.Abstract: Discussions on gender are livelier than ever. For this very reason, they deserve our attention. For a balanced approach, we should start with the mysteries of the body and of sexuality. Despite appearances to the contrary, the body and sexuality are dynamic realities, subject to more or less constant change. Biotechnology further highlights this possibility of changes that until recently were either spontaneous or effected through more or less conventional means. Thus, they cannot be ignored. The most important issue seems to point to a serene dialogue, through which we can dive a little deeper into the human condition. Every radicalization tends to distort reality. The search for a balance is crucial at this time when the temptation of reductionism is present in all human realities. But the balance can only be found in so far as we can value both the esse and the fieri: i.e, that which changes and that which remains.Keywords: Gender. Body. Sexuality. Biotechnologies. Changes.
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Cheng, Xiaoling, Tongtong Wang, Zhibin Yin, and Wei Hang. "Single-cell imaging of AuNPs and AgNPs by near-field desorption ionization mass spectrometry." Journal of Analytical Atomic Spectrometry 35, no. 5 (2020): 927–32. http://dx.doi.org/10.1039/c9ja00389d.

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18

Vestal, Tom Andrew, and Gary E. Briers. "419 Knowledge, Attitudes, and Perceptions of Journalists for Newspapers in Metropolitan Markets in the United States Regarding Food Biotechnology." HortScience 34, no. 3 (June 1999): 516C—516. http://dx.doi.org/10.21273/hortsci.34.3.516c.

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This study enhances knowledge of and information for food systems educators and industry about multiplying their efforts—enlisting collaboration of journalists and the social institution of mass media—in educating consumers about food biotechnology. The focus of this study (diffusion of innovations of food biotechnology) may change behaviors of researchers, agricultural educators, and those in the food biotechnology industry. The researchers investigated journalists' knowledge about, attitudes toward, and perceptions of food biotechnology. Eighty-eight journalists practicing at the nation's largest newspapers and representing “beats” of business, environment, agribusiness, features, food, health/medical, and science/technology provided data for the study. A researcher-developed instrument measured journalists' knowledge, journalists' attitudes (acceptance of genetically modified organisms, acceptance of food biotech practices, effects of biotechnology, level of importance of research, faith in sources, level of importance placed on investigative reporting style, and fear of using food biotechnology), and journalists' perceptions regarding acceptance of food biotechnology as a farm practice. Major findings were journalists' knowledge was low (mean 30.2%), most journalists considered genetic modification of plants as “acceptable,” journalists had greatest faith in “university scientists” and “health professionals” as sources of biotechnology information, journalists do further investigation and interpretation of information given by sources based on their faith in the source, journalists believed that farmers would accept food biotechnology more rapidly than consumers, journalists with higher perceived scientific knowledge had greater acceptance of genetically modified organisms, journalists with more knowledge about biotechnology saw fewer obstacles to acceptance of food biotechnology; and “Writers” rather than “Editors/Managers” accepted more readily genetically modified organisms, had greater faith in sources, had less fear of using food biotechnology, and perceived a more rapid rate of acceptance of food biotechnology as a farm practice.
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19

Bauer, M. W. "Public Perceptions and Mass Media in the Biotechnology Controversy." International Journal of Public Opinion Research 17, no. 1 (March 1, 2005): 5–22. http://dx.doi.org/10.1093/ijpor/edh054.

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20

Tami, Joseph A. "Major Techniques of Biotechnology." Journal of Pharmacy Practice 11, no. 1 (February 1998): 28–37. http://dx.doi.org/10.1177/089719009801100106.

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Since the discovery of the structure and function of DNA over 40 years ago, the established knowledge of molecular biology has increased dramatically, and many new tools have been discovered and utilized by scientists to develop new therapeutic agents. Important tools that are used in recombinant DNA technology include restriction endonucleases (cleave DNA), DNA ligase (link DNA molecules together), and cloning vectors (place foreign DNA into an organism such as bacterial or yeast cells in order to mass produce the protein encoded by that foreign DNA). The development of hybridoma technology provided a method to produce virtually unlimited quantities of pure antibody with a single specificity. These immuno-globulins are known as monoclonal antibodies, and have provided both therapeutic and diagnostic agents. Antisense molecules are oligonucleotides which bind to the messenger RNA (mRNA) of a target gene and selectively inhibit the production of specific proteins. Potential applications for these molecules include cancer and viral and inflammatory diseases. The more recent development of the polymerase chain reaction (PCR) has provided a tool that has revolutionized diagnostic testing in diverse areas such as infectious diseases, genetic abnormalities, and cancer.
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Bonfadelli, Heinz, Urs Dahinden, and Martina Leonarz. "Biotechnology in Switzerland: high on the public agenda, but only moderate support." Public Understanding of Science 11, no. 2 (April 2002): 113–30. http://dx.doi.org/10.1088/0963-6625/11/2/302.

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In Switzerland, there have been intensive public debates about biotechnology because of the specific Swiss political system of direct democracy that led, in 1992 and 1998, to two national referenda on biotechnology regulation. As a result, the Swiss population is well informed but skeptical about this technology. These findings contrast with the deficit model of public understanding of science, which predicts a positive correlation between knowledge of, and support for, a specific technology. What role did the media play in the development of public opinion? This question is discussed because of a content analysis (time series) of national newspapers. In addition, representative surveys and focus groups yield insights into the public perception of biotechnology and the influence of mass communication for opinion formation.
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Schalley, Christoph A. "The expanding role of mass spectrometry in biotechnology Gary Siuzdak." Journal of the American Society for Mass Spectrometry 15, no. 4 (April 2004): 625. http://dx.doi.org/10.1016/j.jasms.2003.12.009.

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Anita Das Ravindranath. "BIOTECHNOLOGY IN COIR EXTRACTION & WASTE UTILIZATION." CORD 17, no. 02 (June 1, 2001): 34. http://dx.doi.org/10.37833/cord.v17i02.349.

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Coir, the fibre extracted from the mesocarp of the drupe of the coconut palm cocos nucifera is used for the production of beautiful products like mats, matting, carpet, handicrafts etc. (Table I) Coir suits very well for geotextiles and insulating materials and being environment friendly has an edge over synthetics for application in environmental operations.
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Tanzli, Ewin, and Andrea Ehrmann. "Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth." Applied Sciences 11, no. 15 (July 28, 2021): 6929. http://dx.doi.org/10.3390/app11156929.

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In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications.
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Eliyanti, Eliyanti, Hasanuddin Hasanuddin, and Mudatsir Mudatsir. "Penerapan Handout Berbasis Pendekatan Sets (Science, Environment, Technology, And Society) pada Materi Bioteknologi Terhadap Hasil Belajar Siswa MAS Darul Ihsan Aceh Besar." BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan 6, no. 2 (November 28, 2019): 105. http://dx.doi.org/10.22373/biotik.v6i2.5615.

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A research to investigate the improvement of student learning outcomes on Biotechnology concept with the application of SETS (Science, Environment, Technology and Society) based handout has been carried out at MAS DarulIhsan Aceh Besar on the second semester of the Academic Year 2017/2018. The research used The Pre-experimental with One Group Pretest-Posttest Design. The samples were 32 students of class XII/b MAS Darul Ihsan Aceh Besar. The data were collected by using multiple choice questions. The results showed that student learning outcomes were increased after the implementation of handout based on SETS. It can be concluded that the application of handout that is based on SETS approach can improve students learning outcomes on Biotechnology concept in MAS Darul Ihsan Aceh Besar.
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Díaz-Alejo, Héctor M., Victoria López-Rodas, Camino García-Balboa, Francisco Tarín, Ana I. Barrado, Estefanía Conde, and Eduardo Costas. "The Upcoming 6Li Isotope Requirements Might Be Supplied by a Microalgal Enrichment Process." Microorganisms 9, no. 8 (August 17, 2021): 1753. http://dx.doi.org/10.3390/microorganisms9081753.

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Lithium isotopes are essential for nuclear energy, but new enrichment methods are required. In this study, we considered biotechnology as a possibility. We assessed the Li fractionation capabilities of three Chlorophyte strains: Chlamydomonas reinhardtii, Tetraselmis mediterranea, and a freshwater Chlorophyte, Desmodesmus sp. These species were cultured in Li containing media and were analysed just after inoculation and after 3, 12, and 27 days. Li mass was determined using a Inductively Coupled Plasma Mass Spectrometer, and the isotope compositions were measured on a Thermo Element XR Inductively Coupled Plasma Mass Spectrometer. The maximum Li capture was observed at day 27 with C. reinhardtii (31.66 µg/g). Desmodesmus sp. reached the greatest Li fractionation, (δ6 = 85.4‰). All strains fractionated preferentially towards 6Li. More studies are required to find fitter species and to establish the optimal conditions for Li capture and fractionation. Nevertheless, this is the first step for a microalgal nuclear biotechnology.
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Borzooeian, Zahra, Mohammad E. Taslim, Saina Rezvani, and Giti Borzooeian. "A high precision length-based carbon nanotube ladder." RSC Advances 8, no. 63 (2018): 36049–55. http://dx.doi.org/10.1039/c8ra05482g.

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Today, carbon nanotubes manufacturers as well as users such as molecular electronics, nanomedicine, nano-biotechnology and similar industries are facing a major challenge: lack of length uniformity of carbon nanotubes in mass production.
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Pizzuto, Matteo Samuele, Fabrizia Zatta, Andrea Minola, Alessia Peter, Katja Culap, Siro Bianchi, Leah Soriaga, et al. "1231. VIR-2482: A potent and broadly neutralizing antibody for the prophylaxis of influenza A illness." Open Forum Infectious Diseases 7, Supplement_1 (October 1, 2020): S635—S636. http://dx.doi.org/10.1093/ofid/ofaa439.1416.

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Abstract Background Influenza A viruses are responsible for seasonal epidemics and represent a constant pandemic threat. Influenza vaccines induce predominantly antibodies against the head region of hemagglutinin (HA) and are strain specific. Vaccine effectiveness is often suboptimal due to mismatch with drifting viruses and an inadequate immune response. Broadly neutralizing monoclonal antibodies (mAbs) targeting the conserved stem-region of HA may provide protection through multiple seasons and cover strains with pandemic potential. We report pre-clinical data on VIR-2482, a fully human anti-HA stem mAb with half-life extending Fc mutations. Methods Binding of VIR-2482 to a panel of influenza HAs and neutralization of H1N1 and H3N2 viruses were measured by ELISA and microneutralization. Epitope conservation was evaluated using 49,462 HA sequences retrieved from GiSAID. Engagement of human FcγRs by VIR-2482 was assessed by biolayer interferometry. Antibody-dependendent cell-mediated cytoxicity (ADCC) was measured via in vitro killing of A549 cells expressing H1-HA glycoprotein by human NK cells. Complement-dependent cytotoxicity (CDC) was evaluated by incubating VIR-2482 with H1N1 infected cells in the presence of guinea pig complement. Protection studies were performed in Balb/c mice given VIR-2482 24h before intranasal infection with a lethal dose of H1N1 PR8 and H3N2 HK/68. Results VIR-2482 binds to the HA proteins representing all 18 influenza A HA subtypes and neutralizes a broad panel of H1N1 and H3N2 viruses spanning almost 100 years of evolution. Bioinformatic analysis revealed >98.8% conservation for the majority of key contact residues examined from sequences retrieved for H1N1 and H3N2 between 2009-2019. The half-life extending mutations in the Fc portion do not affect the ability of the antibody to engage FcγRIIIa, FcγRIIa, and C1q as evidenced by their lack of impact on ADCC and CDC in vitro. Prophylactic administration of VIR-2482 protects Balb/c mice from infection with lethal challenge doses of H1N1 and H3N2 viruses. Conclusion The attributes of potency, broad recognition of a highly conserved epitope, retention of high-level effector functions in addition to half-life extension support the development of VIR-2482 as a universal prophylactic for influenza A illness. Disclosures Matteo Samuele Pizzuto, PhD, VIR Biotechnology (Employee) Fabrizia Zatta, n/a, Vir Biotechnology (Employee) Andrea Minola, MS, Vir Biotechnology (Employee) Alessia Peter, n/a, Vir Biotechnology (Employee) Katja Culap, n/a, Vir Biotechnology (Employee) Leah Soriaga, PhD, Vir Biotechnology (Employee) Anna De Marco, n/a, Vir Biotechnology (Employee) Barbara Guarino, PhD, Vir Biotechnology (Employee) Nadia Passini, n/a, Vir Biotechnology (Employee) David K. Hong, MD, Vir Biotechnology (Employee) Fabio Benigni, PhD, Vir Biotechnology (Employee) Christy Hebner, PhD, Vir Biotechnology (Employee) Aurelio Bonavia, PhD, Vir Biotechnology (Employee) Davide Corti, PhD, Vir Biotechnology (Employee)
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Russell, Alan M. "International Relations Theory, Biotechnology, and War." Politics and the Life Sciences 9, no. 1 (August 1990): 3–19. http://dx.doi.org/10.1017/s0730938400010182.

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As a field of study international relations has always been concerned with the problem of war. Since the second world war much attention has been given to the impact of weapons of mass destruction, most commonly in the form of nuclear weapons. More recently, a wider variety of issues have been addressed by the field, notably widening its coverage of economic, welfare and social issues. Three broad approaches to international relations have emerged: political realism, pluralism, and globalism.Biotechnology represents a major technological revolution which will have enormous impact on the world at many levels. One impact which cannot be ignored is its potential for weaponry. This paper assesses the three international relations approaches with respect to their elucidation of biotechnology and its potential for violent and aggressive applications.
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Cheliak, W. M., and D. L. Rogers. "Integrating biotechnology into tree improvement programs." Canadian Journal of Forest Research 20, no. 4 (April 1, 1990): 452–63. http://dx.doi.org/10.1139/x90-062.

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Time is a major constraint in the progress of tree improvement programs. Four ways in which time influences the tree improvement process are (i) evolutionary time, (ii) time to harvest, (iii) time to achieve phenotypic stability, and (iv) time to reach reproductive maturity. The ways in which each of these affects the three phases of a tree improvement program (conservation, selection and breeding, and propagation) are identified and discussed. How biotechnological techniques, as well as other enabling technologies, address the time constraint problem is also discussed. The biotechnological approaches include tissue culture, molecular genetics, and genetic engineering; the enabling technologies include early testing and flower induction. Through tissue culture it is possible to increase genetic gain per unit time and increase total genetic gain by using more of the total genetic variation. Development of high-resolution linkage maps, through application of molecular genetics technology, will provide new approaches to early screening, testing, and selection. Additionally, molecular probes will be useful in improving methods that genetically fingerprint germ plasm. Genetic engineering has considerable potential to reduce time constraints. However, because of the diverse breeding and production populations typically employed, much basic work needs to be done to integrate genetically engineered materials into tree improvement programs. Early selection and flower induction address the time constraints imposed by age-stable performance and reproductive maturity. When used in combination with the previously described biotechnologies, a powerful system is created that can dramatically reduce the time required to integrate genetically improved material into forest regeneration programs. An example of integrating tree improvement, clonal forestry, and biotechnology is described for an existing black spruce regeneration program.
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Salvalaggio, Guilherme Gallas, Eduardo Franz Berkenbrock, Jhonatan Rafael Wendling Hartmann Hister, Eliane Cristina Gruszka Vendruscolo, Hendrel Vinícius Richter Assis, Vivian Carré Missio, Alan Gregório Martins, and Robson Fernando Missio. "SEGREGATION OF THE RR BIOTECHNOLOGY AND AGRONOMICAL VARIABLES IN SINGLE-CROSS, DOUBLE-CROSS, AND F2 HYBRIDS OF MAIZE (ZEA MAYS L.)." International Journal of Research -GRANTHAALAYAH 8, no. 10 (November 16, 2020): 338–49. http://dx.doi.org/10.29121/granthaalayah.v8.i10.2020.2113.

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The present work evaluated the segregation of the RR-biotechnology and the agronomic performance of maize hybrids originated from three seed categories: single-cross commercial hybrids, double-cross hybrids, and saved seeds (F2 population). The experiment was set up in the field, within a producing area used for market purposes, in São Miguel do Iguaçu, Paraná, Brazil. The research followed a randomized block design with four repetitions. We investigated the following treatments: saved seeds/F2 population (MG 580 PW, MG 30A37 PW, MG 600 PW, and MG 545 PW), seeds of single-cross commercial hybrids (MG 580 PW, MG 30A37 PW, AS 1777 PRO3, AG 9000 PRO3, and DKB 290 PRO3) and home-bred seeds/double-cross hybrids (DKB 290 PRO3 x MG 30A37 PW, DKB 290 PRO3 x DKB 285 PEO3, DKB 290 PRO3 x DOW 2B587 PW, and DKB 290 PRO3 x MG 600 PW). The study considered agronomical characters, as well as the segregation of the RR-biotechnology. The hybrids MG 580 PW and DKB 290 PRO3 were the most productive ones, yielding 9,244.31 and 9,151.84 kg ha-1, respectively. The groups formed by double-cross and F2-population hybrids had the highest average values of RR-biotechnology segregation (20.5% and 13%, respectively). The group of commercial hybrids proved to be superior to the others in all variables appraised, and they also did not show segregation of the RR-biotechnology. The principal component analysis made it possible to sort the three categories of seeds. In conclusion, despite the high cost, the commercial seeds stood out showing the best cost-benefit for maize crops.
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Suryaningtyas, Indyaswan Tegar. "APLIKASI BIOTEKNOLOGI MOLEKULER DALAM BUDAYA PERAIRAN." OSEANA 42, no. 4 (December 30, 2017): 13–24. http://dx.doi.org/10.14203/oseana.2017.vol.42no.4.70.

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MOLECULAR BIOTECHNOLOGY APPLICATION IN MARINE AQUACULTURE. Aquaculture industry, as a promising solution for the decrease of natural broodstock as well as an important part in people's health and economics, has been growing rapidly. Biomolecular technologies are now used as a base for proper management of aquaculture programs. Those technologies include breeding program with genetic maps; mono-sex aquaculture program to maximize production's effectiveness; transgenic program; and pathogen's early detection using bio-molecular methods. it can be said that the modern aquaculture industry nowadays depends on the application of molecular biotechnology with support from better aquaculture-system management for product organization.
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33

Bonfadelli, H. "Mass Media and Biotechnology: Knowledge Gaps Within and Between European Countries." International Journal of Public Opinion Research 17, no. 1 (March 1, 2005): 42–62. http://dx.doi.org/10.1093/ijpor/edh056.

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34

Horst, Maja. "Cloning sensations: mass mediated articulation of social responses to controversial biotechnology." Public Understanding of Science 14, no. 2 (April 2005): 185–200. http://dx.doi.org/10.1177/0963662505050994.

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35

Aswath, Chenna Reddy, and Sun Hyung Kim. "Another Story of MADS-Box Genes – their Potential in Plant Biotechnology." Plant Growth Regulation 46, no. 2 (June 2005): 177–88. http://dx.doi.org/10.1007/s10725-005-8482-7.

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36

Kossovsky, Nir, and David Millett. "Materials Biotechnology and Blood Substitutes." MRS Bulletin 16, no. 9 (September 1991): 78–81. http://dx.doi.org/10.1557/s0883769400056104.

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Blood is a dispersion of formed elements in an aqueous colloid. The combined mass of the formed elements of blood measure on average 30 ml per kg body weight, or about the same weight as the liver. The colloidal phase of blood contains numerous organic factors that play important primary and supporting roles in homeostasis, including immune surveillance, coagulation, and nutrient transport.Erythrocytes (red blood cells) are the principle formed elements and provide the life-sustaining function, in conjunction with the heart, lungs, blood vessels and kidneys, of transporting and protecting the oxygen-carrying pigment, hemoglobin, to the tissues. The oxygen-binding properties of hemoglobin are sensitive to factors such as the cooperative effects of O2 binding, pH and CO2 levels, and the presence of other metabolic intermediates such as 2,3-diphosphoglycerate. The synergistic effects of these factors produce a well-known sigmoidal curve plot of the relationship between oxygen affinity and the partial pressure of oxygen (pO2): there is high oxygen affinity in the lung where the pO2 is high, and a low oxygen affinity in the tissues, where the pO2 is low. Uptake and delivery of oxygen by hemoglobin is associated with considerable spatial rearrangement of the hemoglobin molecule.Blood is a non-Newtonian suspension. Its viscosity is a function of both the vascular diameter and the concentration of erythrocytes. At a normal hematocrit of 40%, the viscosity of blood ranges between 2 and 4 Pa s as measured in tubes ranging 10–1,500 μm diameter. The osmolality of blood serum is 275–295 mOsm/1.
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37

Wnuk, Agnieszka, and Marcin Kozak. "Knowledge about and Attitudes to GMOs among Students from Various Specializations." Outlook on Agriculture 40, no. 4 (December 2011): 337–42. http://dx.doi.org/10.5367/oa.2011.0064.

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Genetically modified organisms (GMOs) are a hot topic for debate among researchers from a wide range of scientific disciplines – in particular, agriculture, biotechnology and environmental protection. The debate, however, is not limited to science. GMOs are also discussed in the mass media, giving rise to extreme emotions, both positive and negative. Without entering the argument between the supporters and opponents of GMOs, this paper addresses the following questions. How do future experts in agriculture, biotechnology and environmental protection approach the topic of GMOs? Do they have similar or dramatically different views on this subject? What do they know about GMOs? Is their knowledge related to their attitudes? University students specializing in those three disciplines can be considered as future experts in the field, and so a questionnaire survey was conducted among them to answer the above questions. The results show that agricultural, biotechnology and environmental protection students differ in their attitudes to and knowledge about GMOs.
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38

Soewandi, Bayu Dewantoro Putro. "Application of Reproduction Biotechnology and Molecular Genetic to Improve Productivity of Local Pigs." Indonesian Bulletin of Animal and Veterinary Sciences 27, no. 4 (March 3, 2018): 177. http://dx.doi.org/10.14334/wartazoa.v27i4.1677.

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Low productivity in local pigs is due to the absence of breeding plan with mating arrangements. The main productivity variables used in breeding are litter size (number of piglets), weaning litter, number of teats, sow productivity index, growth rate, slaughter weight, and carcass quality. Selection to produce a new breed in breeding scheme should be conducted within five generations requiring large numbers of livestock, length period, and costly. Biotechnology reproduction and genetic molecular can be used to increase the value of the variables, to shorten interval generation, and cost efficient. This paper provides information on the use of reproduction biotechnology and molecular genetic from various sources and utilization of both technologies to increase local pig productivity. Reproductive biotechnology is useful in mating arrangement and molecular genetic using marker assisted selection (MAS) is able to determine superior pig since early age.
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39

Bellon, Anne, Alain Sadoun, Karine Grivel, Maxime Moulard, Frederique Brune, Jean-Francois Prost, and Margarita Salcedo. "Comparison of Cell Lysis Mediated by LFB-R603 with That Mediated by Ofatumumab Against Cells Expressing Low Levels of CD20,." Blood 118, no. 21 (November 18, 2011): 3913. http://dx.doi.org/10.1182/blood.v118.21.3913.3913.

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Abstract Abstract 3913 Anti-CD20 monoclonal antibodies (mAb) eliminate CD20-positive cells mainly through three different molecular mechanisms including antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) and apoptosis. They are indicated as monotherapy or in combination with other compounds to treat B-cell malignancies such as Chronic Lymphocytic Leukemia (CLL). In contrast to normal B-lymphocytes, B malignant cells in CLL patients usually express low levels of CD20 molecules at their cell surface. Rituximab (Rituxan®/MabThera®), the first-in-class anti-CD20 mAb, has been described to be poorly active in CLL patients as a single agent. Ofatumumab (Arzerra®), the second generation approved anti-CD20 mAb, displays a higher CDC-activity when compared to rituximab. However, the contribution of this mechanism of action to control this disease is not fully characterized and may not be sustained when low CD20-density malignant cells are targeted. We have developed a third generation anti-CD20 mAb (LFB-R603) on our EMABling® platform, known to select antibodies with improved ADCC, based on its glycosylation pattern. We have previously demonstrated that LFB-R603 is able to mediate a more potent ADCC against several cell lines as compared to rituximab. In this study, we further investigated lysis activity of LFB-R603 as compared to ofatumumab and rituximab. In terms of CDC-mediated lysis, we observed a higher CDC activity of ofatumumab compared to LFB-R603 on several cell lines (Raji, MEC-1, Wil-2S) expressing high CD20 levels. Using a FACS-based methodology and MEC-1 cells as targets, C1q-dependent-CDC triggered by ofatumumab was shown to induce approximately 90% of specific cell-lysis as compared to 60% cell-lysis triggered by LFB-R603. Rituximab displayed a CDC activity slightly superior to that of LFB-R603. In contrast, CDC activity against CD20-low expressing cells, illustrated by SUDHL-8, was very low and almost identical with all three mAbs, rituximab, ofatumumab and LFB-R603. Indeed, ofatumumab was only able to mediate 5% CDC lysis, comparable to the 1% observed with LFB-R603. Regarding the ADCC efficacy, and consistently to previous results, LFB-R603 was shown to mediate ADCC at a very high rate against all cell lines tested. Around 55% lysis of LFB-R603-opsonized MEC-1 was obtained using PBMC as effectors from 5 independent healthy donors, while less than 30% lysis of rituximab- or ofatumumab-opsonized MEC-1 was observed using the same PBMC samples. In addition, LFB-R603 still mediated a potent ADCC against CD20-low expressing cells (SUDHL-8 and JYE5.1), whereas both ofatumumab and rituximab mediated ADCC at low levels. Indeed, only 5% of rituximab-opsonized SUDHL-8 cells were killed by PBMC from healthy donor, whereas around 30% of the SUDHL-8 opsonized by LFB-R603 were lysed by the same effectors. Altogether, these results support the fact that the therapeutic use of LFB-R603 may be advantageous over currently approved anti-CD20 mAbs to target malignant cells where surface CD20 molecules are known to be expressed at low levels such as CLL and potentially other lymphoma subtypes. Disclosures: Bellon: LFB Biotechnologies: Employment. Sadoun:LFB Biotechnologies: Employment. Grivel:LFB Biotechnologies: Consultancy. Moulard:LFB Biotechnologies: Consultancy. Brune:LFB Biotechnologies: Employment. Prost:LFB Biotechnologies: Employment. Salcedo:LFB Biotechnologies: Employment.
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40

MALLARD, J., and J. C. MOCQUOT. "Insémination artificielle et production laitière bovine : répercussions d’une biotechnologie sur une filière de production." INRAE Productions Animales 11, no. 1 (February 2, 1998): 33–39. http://dx.doi.org/10.20870/productions-animales.1998.11.1.3914.

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L’insémination artificielle à partir du sperme congelé de taureau est une des plus anciennes biotechnologies, apparue dans les années 40. Elle a induit des modifications profondes dans les pratiques et les structures de la filière bovine laitière. L’article en fait un recensement, en allant des plus immédiates aux plus indirectes. Elles vont de la création de marges liées à la disparition des taureaux de l’exploitation jusqu’à l’intégration de l’éleveur dans un réseau de relations professionnelles. Il devient ainsi un acteur et le bénéficiaire d’une sélection collective particulièrement efficace. Mais ce canal ouvre au-delà la voie à la pénétration du progrès technique et à la prise en charge des intérêts collectifs. On essaie de démontrer que ce ne sont pas forcément les conséquences les plus directes qui ont été les plus importantes, concluant qu’il est sans doute bien difficile de prévoir l’impact de biotechnologies récentes.
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41

Pohl, P., M. Kohlhase, and M. Martin. "Pilot Scale Axenic Mass Cultivation of Microalgae I. Development of the Biotechnology." Planta Medica 52, no. 05 (October 1986): 416–17. http://dx.doi.org/10.1055/s-2007-969214.

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42

Carolan, Michael S. "Mapping Biotechnology: From Epistemic Artifacts to Geographies of Control." Nature and Culture 2, no. 2 (September 1, 2007): 115–38. http://dx.doi.org/10.3167/nc.2007.020202.

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This article maps key epistemological and ontological terrains associated with biotechnology. Beginning with the epistemological, a comparison is made between the scientific representations of today, particularly as found in the genomic sciences, and the scientific representations of the past. In doing this, we find these representations have changed over the centuries, which has been of significant consequence in terms of giving shape to today's global political economy. In the following section, the sociopolitical effects of biotechnology are discussed, particularly in terms of how the aforementioned representations give shape to global path dependencies. By examining the epistemological and ontological assumptions that give shape to the global distribution of informational and biological resources, this article seeks to add to our understanding of today's bioeconomy and the geographies of control it helps to create.
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43

Chupin, D. "LE ROLE DES BIOTECHNOLOGIES DE LA REPRODUCTION POUR LA CONSERVATION DES RESSOURCES GENETIQUES ANIMALES." Animal Genetic Resources Information 14 (April 1994): 13–25. http://dx.doi.org/10.1017/s101423390000451x.

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RESUMECet article analyse dans quelle mesure les nouvelles perspectives offertes par les biotechnologies de la reproduction s'appliquent aux activités de conservation des ressources génétiques animales. Ces activités ont été regroupées autour de cinq thémes: Décrire, Détruire, Défendre, Stocker, Recréer. Ces techniques n'apportent aucun plus pour la description des caractéristiques d'une population ou d'une race. La principals menace qui pése sur une race d'animaux domestiques est de disparaitre par croisement avec une autre, et l'insémination artificielle représente de ce point de vue un danger certain. Inversement, l'insémination artificielle peut devenir un instrument de défense des races locales. II serait plus difficile de trouver une justification A l'utilisation de la plupart des autres biotechnologies (transfert d'embryons, sexage, clonage) pour la protection et la promotion des races locales. Pour la conservation des ressources génétiques, la cryoconservation des spermatozdides et des embryons, mais également les ovocytes, voire des morceaux de gonades et des cellules somatiques est un outil irremplagable. La demiére partie présente des calculs sur les possibilités de re-création de races, selon la nature du matériel stocks.
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44

Sidashova, S. O., V. F. Stachovsky, and О. V. Shcherbak. "RESEARCH OF LIVE MASS OF CALF–EMBRYOTRANSLANTANTS." Animal Breeding and Genetics 57 (April 24, 2019): 185–91. http://dx.doi.org/10.31073/abg.57.22.

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The efficiency of breeding and production processes in dairy cattle breeding are closely linked and limited by the genetic value of animals, their reproduction ratio, and the rate of change of generations. The use transplantation of embryo in the practice of dairy cattle breeding ensures the effective implementation of modern reproductive biotechnology, which will promote the expanded reproduction of the most valuable, tested animal complexes. Goal. To study the dynamics of live weight of calves-embryo-transplants and compare them with analogues obtained after artificial insemination, in conditions of farms of different climatic regions of Ukraine. Methods. Zotechnical, statistical, analytical. Analytical part of the work was carried out on the retrospective data of zootechnical records in three farms, in the warehouse of which were introduced repair heifers-embryotransplantants. Biotechnological procedures (in vivo) for embryocollection, transplantation of fresh and frozen-thawed embryos were carried out in accordance with well-known techniques. Materials for system analysis were collected during the period of practical activity of the Laboratory of embryo transplantation at PJSC "Poltavapleservis" and in the period after its reorganization. To study the dynamics of the live weight of repair calves-embryo-transplants in three different climatic and technological conditions farms were selected analogue groups of calves of the corresponding age and breed (in the ALLC "Petrodolynske", the comparison was made with Ukrainian red dairy breeds) born after fertilization artificial insemination of heifers. Results. It was established that the live weight of newborn calves-embryo-transplants of dairy breeds was lower compared to analogues by 6.8% (with fluctuations of 0.86–5.46 kg). Heifers-embryo-transplants yielded to live weight analogues for the entire period of the survey, but had a higher rate of growth. At 9 months of age, the difference in live weight was 4.7%, but the difference in farms of different climatic regions increased significantly: from 0.96 to 8.72%. Conclusions. The tendency of the dependence of the dynamics of the live weight of heifers on the methods of reproduction biotechnology, as well as on the effects of the conditions of retention, is established. Some elements of this trend require further study, in order to optimize the technology of growing a repair stock with high genetic potential.
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45

Hong, Yan. "Agriculture — Forest Biotechnology is Beginning to Take Root." Asia-Pacific Biotech News 10, no. 09 (May 15, 2006): 507–9. http://dx.doi.org/10.1142/s0219030306000693.

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With the increasing loss of forests, the need for their renewal by natural means is imminent. Thanks to the Kyoto treaty demanding reduction in greenhouse gases, plantations of fast growing and high yielding tree species have thrived around the world. In the past few years, there has been a lot of progress in the area of forest biotechnology. Genetic modification (GM) has introduced desirable traits such as insect tolerance, herbicide resistance, sterility, better pulping quality and fast growth. The plantation of GM trees, however, raises the concerns of possible damage to the environment, impact to biodiversity and possible gene flow into natural forests. These issues need to be addressed before there is any wide acceptance of GM trees. Other non-GM biotechnology techniques such as mass propagation of elite tree individuals through meristematic propagation or somatic embryogenesis are more acceptable and have been widely used.
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46

VIGNON, X., Y. HEYMAN, P. CHAVATTE-PALMER, and J. P. RENARD. "Biotechnologies de la reproduction : le clonage des animaux d’élevage." INRAE Productions Animales 21, no. 1 (March 20, 2008): 33–44. http://dx.doi.org/10.20870/productions-animales.2008.21.1.3373.

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La reproduction d’espèces domestiques par clonage remonte à plus de 20 ans. Les premières techniques reposent sur la bissection d’embryons puis sur l’usage de cellules embryonnaires pour générer des animaux clonés par transfert de noyau. Avec l’apparition du clonage somatique, qui permet la préservation et l’obtention de descendants de génotypes de haute valeur, l’intérêt pour cette technologie s’est considérablement accru. Un retour sur ces 20 dernières années montre comment l’INRA s’est impliqué dans la mise au point méthodologique du clonage animal et a acquis une expertise reconnue, non seulement dans la production et l’analyse de clones d’animaux domestiques, mais également dans l’étude du rôle des altérations génétiques et épigénétiques dans le développement embryonnaire, foetal et postnatal chez ces animaux. Le clonage reste en effet peu efficace pour la production d’individus viables en comparaison d’autres techniques de reproduction assistée. Une meilleure compréhension de la reprogrammation épigénétique des noyaux de cellules somatiques et de ses effets sur le déroulement des gestations est nécessaire pour rendre cette technologie éthiquement et économiquement acceptable. L’acceptation des produits de clones dans l’alimentation dépend maintenant de l’approbation des agences d’évaluation. Celle-ci est basée sur l’acquisition de données scientifiques concernant la sécurité et l’équivalence des produits avec ceux qui sont issus d’animaux reproduits par fécondation. Cette synthèse fait le point sur les principales connaissances fondamentales sur le clonage somatique, depuis la cellule donneuse, jusqu’au descendant cloné, et leurs liens avec la santé, la viabilité, les caractéristiques quantitatives et de reproduction des individus clonés.
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47

Schäfer, Mathias. "Book Review: The Expanding Role of Mass Spectrometry in Biotechnology. By Gary Siuzdak." ChemBioChem 5, no. 6 (May 26, 2004): 887. http://dx.doi.org/10.1002/cbic.200300152.

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48

Chaumont, Daniel. "Biotechnology of algal biomass production: a review of systems for outdoor mass culture." Journal of Applied Phycology 5, no. 6 (December 1993): 593–604. http://dx.doi.org/10.1007/bf02184638.

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49

Jebril, Nadia Mahmoud Tawfiq Jebril. "Novel use of XRF in the adsorption processes for the direct analysis of cadmium and silver in absorbent Na-alginate beads." Baghdad Science Journal 17, no. 4 (December 1, 2020): 1139. http://dx.doi.org/10.21123/bsj.2020.17.4.1139.

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The Na-alginate bead is commonly used in biotechnology fields such as adsorption due to ion exchange between Ca and Na with elements. Scanning electron microscopy (SEM-EDX) has proven to be a comparative method in the detections of these adsorbed elements, but the un-flat forming area of beads that can introduce impossible of the detection of element adsorbed. In contrast, X-ray fluorescence (XRF) documents analysis of elements, direct examination, which may analysis the adsorbents of elements. Here, this Study evaluated the possibility by using XRF for the direct analysis for examples of Cd and Ag in a bench stand. This Study compared this to commonly used SEM-EDX and inductively coupled plasma mass spectrometry (ICP-MS) approaches for Cd and Ag analyses. Overall, novel use of XRF provided precise analyses of the concentrations of Cd and Ag adsorbed within Na-alginate bead and revealed analyses not determined the concentrations in standard SEM-EDX or previously established in other studies of Na-alginate beads. In addition, this Study shows that XRF may be employed in different fields in biotechnology to analysis the concentrations regarding the elemental adsorption of Na-alginate beads and demonstrate the potential for XRF to monitor the procedures underpinning biotechnology sectors.
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50

Petrova, T. A., K. O. Sidorov, Yu G. Il’yinova, and I. A. Narkevich. "Venture financing in the segment of pharmaceutical biotechnology in the Russian Federation." Medical Almanac, no. 2 (June 16, 2019): 35–39. http://dx.doi.org/10.21145/2499-9954-2019-2-35-39.

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One of the main directions of the pharmaceutical industry is the transition to the stage of commercialization of the results of intellectual activity and to the large-scale creation of global markets for new products and services. In recent years, the number of research conducted in the pharmaceutical industry has increased, and the most acute question is the active introduction of research results into mass production. The article provides an overview of the current state of venture financing in the biotechnology industry of the Russian Federation, as the most effective mechanism for financing promising applied research and, in particular, in the pharmaceutical biotechnology segment. The authors reviewed the main program documents affecting the development of biotechnology in Russia. The main venture funds that invest in pharmaceutical biotech companies are identified. The assortment portfolio of invested companies developed for the treatment of certain diseases was considered, and the main direction for the study was determined. The analysis of the funds that carry out the grant support of applied biotechnological research has been carried out. It allows to overcome the «sowing» stage of development of an innovative company and get initial research results. The main reasons for the slow development of venture capital investments in the biotech industry are identified and ways to overcome them are proposed.
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