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1
Sudarmonowati, Enny, I. Fitryatmi, and S. Sadjad. "CRYOPRESERVATION OF TRUE-SEED AND EMBRYO OF MAIZE AND SOYBEAN FOR LONG-TERM STORAGE." Indonesian Journal of Agricultural Science 2, no. 2 (2016): 31. http://dx.doi.org/10.21082/ijas.v2n2.2001.31-36.
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<br />Study on cryopreservation of Indonesian local cultivars and improved varieties of maize and soybean has never been done. This method may be used for long-term preservation of seeds of maize and soybean. In this study, the method was applied to maize and soybean, Arjuna and Wilis respectively, as a model for preserving germplasm of ortodox seeds. Whole seeds and excised embryos of both varieties were subjected to two methods of cryopreservation, i.e., two-stage cooling and rapid freezing with or without 15% dimethyl sulfoxide (DMSO) as cryoprotectant solution prior to immersion in liquid nitrogen (-196oC). Results indicated that there was no significant difference between the use of DMSO for both species in terms of viability, although pretreatment in DMSO was slightly reduced the percentage of viability of both species. Slow freezing to -30oC prior to immersion in the liquid nitrogen could give as high as 76.67% and 51.67% surviving whole seeds of maize and soybean, respectively. Preserving excised embryos of maize in the liquid nitrogen using either slow or rapid freezing significantly reduced the percentage of viability from 20-76.67% to 5-18.33% (four folds) depending on treatments applied. Results also showed that one day or 15 minutes of immersion of samples in the liquid nitrogen gave rise to similar values of viability of maize and soybean, i.e., 20-60% and 20-51.67%, respectively depending on treatments applied. These results implied that for long-term storage of maize and soybean seeds as they could survive at the rate of 76.67% and 51.67% respectively, the seed can be treated by prefreezing to -30oC<br />without the presence of DMSO prior to immersion in liquid nitrogen.<br /><br />
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Sudarmonowati, Enny, I. Fitryatmi, and S. Sadjad. "CRYOPRESERVATION OF TRUE-SEED AND EMBRYO OF MAIZE AND SOYBEAN FOR LONG-TERM STORAGE." Indonesian Journal of Agricultural Science 2, no. 2 (2016): 31. http://dx.doi.org/10.21082/ijas.v2n2.2001.p31-36.
Full textAbstract:
<br />Study on cryopreservation of Indonesian local cultivars and improved varieties of maize and soybean has never been done. This method may be used for long-term preservation of seeds of maize and soybean. In this study, the method was applied to maize and soybean, Arjuna and Wilis respectively, as a model for preserving germplasm of ortodox seeds. Whole seeds and excised embryos of both varieties were subjected to two methods of cryopreservation, i.e., two-stage cooling and rapid freezing with or without 15% dimethyl sulfoxide (DMSO) as cryoprotectant solution prior to immersion in liquid nitrogen (-196oC). Results indicated that there was no significant difference between the use of DMSO for both species in terms of viability, although pretreatment in DMSO was slightly reduced the percentage of viability of both species. Slow freezing to -30oC prior to immersion in the liquid nitrogen could give as high as 76.67% and 51.67% surviving whole seeds of maize and soybean, respectively. Preserving excised embryos of maize in the liquid nitrogen using either slow or rapid freezing significantly reduced the percentage of viability from 20-76.67% to 5-18.33% (four folds) depending on treatments applied. Results also showed that one day or 15 minutes of immersion of samples in the liquid nitrogen gave rise to similar values of viability of maize and soybean, i.e., 20-60% and 20-51.67%, respectively depending on treatments applied. These results implied that for long-term storage of maize and soybean seeds as they could survive at the rate of 76.67% and 51.67% respectively, the seed can be treated by prefreezing to -30oC<br />without the presence of DMSO prior to immersion in liquid nitrogen.<br /><br />
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Nerling, Daniele, Cileide Maria Medeiros Coelho, and Adriele Brümmer. "Biochemical profiling and its role in physiological quality of maize seeds." Journal of Seed Science 40, no. 1 (2018): 7–15. http://dx.doi.org/10.1590/2317-1545v40n1172734.
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Abstract: Viability and vigor of seeds are physiological attributes influenced by a myriad of factors including the genotype. Biochemical profiling of maize seeds and its correlation with their physiological quality is of crucial importance in breeding programs that the aim is seeds of better quality. The main goal of this study was to analyze the genetic diversity among maize inbred lines and evaluate the biochemical components that have an important role in physiological quality of seeds. The seeds of the inbred lines and hybrids were submitted to viability test, vigor by the accelerated aging and cold tolerance, and biochemical profiling (total protein content, soluble proteins, total phosphorus, phytate, inorganic phosphorus, starch and soluble sugars). The genetic divergence between the inbred lines found, prompt us to conclude that genetic gains may exist in association with physiological quality of maize hybrids. Seeds with higher inorganic phosphorus and soluble sugars presented superior physiological quality than seeds with lower levels of those compounds.
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Tamindžić, Gordana, Maja Ignjatov, Dragana Milošević, et al. "Assessment of quality and viability of primed maize seed." Ratarstvo i povrtarstvo 57, no. 3 (2020): 87–92. http://dx.doi.org/10.5937/ratpov57-26575.
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Good crop establishment is essential for achieving high yield and constraints to good establishment include untimely sowing and low seed quality combined with various adverse growing conditions after sowing. Seed priming is a pre-sowing technique used for the improvement of germination, reduction of the time from sowing to emergence and improvement of emergence uniformity. Various seed priming techniques, such as hydropriming and priming with zinc, are used nowadays to improve crop establishment. The importance of seed priming with zinc for better germination, improved stand establishment, and higher maize yield are well documented. However, there is still a lack of results on the effects of seed priming with water and zinc on seed quality and viability, given that maize seed can be kept in storage for many years without a significant reduction in germination. The study was aimed to evaluate the effects of seed priming with water and Zn on the quality and viability of the maize seed. In order to evaluate the response of four maize hybrids to priming with water (hydropriming) and 4 mM zinc sulphate, primed seeds were subjected to laboratory tests, namely to the germination test, the cold test, and the accelerated aging test. Both priming treatments increased the seed quality, but the beneficial effect of Zn-priming maintained to a larger extent than hydropriming in cold-treated and aged seeds. The negative effects of hydropriming on the viability o f the aged seed of hybrid NS 4023 imply a possible limitation to deferred sowing of primed maize seed.
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Davidson, K. G. V., S. Sowa, F. D. Moore, and E. E. Roos. "Maize seed response to successive imbibition/dryback cycles: viability and vigour." Seed Science Research 4, no. 4 (1994): 431–37. http://dx.doi.org/10.1017/s0960258500002488.
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AbstractElectroconductivity tests are currently used for seed vigour assessment. They are rapid and simple and after further development, they may yet provide the seed industry with a non-destructive alternative to the standard germination test, which is thoroughly destructive to the sample, time consuming, and expensive. Seed injury, a result of soaking required by the electroconductivity test, was evaluated using high quality Zea mays L. seeds responding to successive imbibition/dryback cycles. If the soaking time is brief, injury to the seeds should be minimal, thus permitting successive tests on the same sample. We tested 5 imbibition/dryback cycles (C) and 5 imbibition periods, or cycle durations (CD) of 2, 4, 6, 7 and 8 h. Dryback periods lasted 5–7 d at room temperature. Seeds were permitted to dry back to 10% moisture. Electroconductivity readings were obtained at the end of each CD for each C. Each treatment (C × CD) sample, n=100 seeds, was germinated at 25°C for 7 d; radicle lengths were measured after 3 d. The experiment was repeated yielding a total of 50 observations. Viability and vigour losses were measured in response to successive C and increasing CD. Five cycles of 6 h each resulted in only a 10% loss of viability, but a 20% loss of relative vigour, confirming that vigour is more sensitive to the testing procedure. Cycles had the greatest effect on loss of seed quality since 45% of the readily leachable electrolytes were lost from the seeds during the first soaking period. There was no interaction between C and CD.
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Nagel, Manuela, and Andreas Börner. "The longevity of crop seeds stored under ambient conditions." Seed Science Research 20, no. 1 (2009): 1–12. http://dx.doi.org/10.1017/s0960258509990213.
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AbstractThe ability of crop seeds to retain their viability over extended periods of uncontrolled temperature and/or relative humidity conditions has not been widely investigated, although this is an important issue for genebank management. We report here the response of 18 crop species to storage for up to 26 years at 20.3 ± 2.3°C and 50.5 ± 6.3% relative humidity. Germination rates decreased in a sigmoid fashion, but the curve parameters were species characteristic. Pea, common bean and maize seeds retained their viability over the longest period (23, 21 and 19 years, respectively). In contrast, chive seeds survived for only 5 years and lettuce for 7 years. In addition to this interspecific variability, there were also indices for intraspecific variability, particularly in bean and chive seeds, just as in collard, lupin, poppy, wheat and maize seeds. A significant correlation was obtained between germination performance in the laboratory and seedling emergence following autumn sowing. Seeds in which oil was the major seed storage component were more short lived, whereas carbohydrates or proteins did not show an effect on seed longevity.
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Trolove, M. R., and C. A. Dowsett. "Yellow bristle grass seed killed in maize silage." New Zealand Plant Protection 68 (January 8, 2015): 442. http://dx.doi.org/10.30843/nzpp.2015.68.5847.
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Yellow bristle grass (Setaria pumila) is a serious weed that reduces dairy farm profitability and could potentially infest productive land throughout New Zealand Supplementary feed is used extensively in the dairy industry and farmers are concerned that yellow bristle grass seed may be spread with maize silage Previous studies showed that its seeds did not survive burial in covered silage stacks after 1 week To further investigate the decline in seed viability mesh bags containing 50 seeds were buried at 0 and/or 400 mm depth in two covered Waikato silage stacks and retrieved after 1 2 3 5 and 7 days Germination prior to seed burial was 69 and viability using tetrazolium staining was 88 Germination was reduced after 1 day to 1236 2 days to 030 3 days to 04 and 5 days to 0 Viability was reduced after 1 week from 50 to 40 Temperature and pH conditions during this study were not considered sufficient for rapid seed decay and therefore it appeared that chemicals released after ensiling were responsible Further investigation into mechanisms behind this rapid decay and its potential for effective weed seed control is warranted
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Hodges, D. Mark, Christiane Charest, and Robert I. Hamilton. "A chilling resistance test for inbred maize lines." Canadian Journal of Plant Science 74, no. 4 (1994): 687–91. http://dx.doi.org/10.4141/cjps94-124.
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A laboratory germination test based on exposure of seeds to a temperature regime derived from natural conditions was developed to determine chilling resistance in maize (Zea mays L.). Seven inbred lines were exposed to a temperature regime approximating Ottawa, Ontario's (Lat. 45° 24′N, Long. 75° 43′W) spring climate. Seeds were subjected to a range of maximum (16 h) and minimum (8 h) temperatures corresponding to the dates spanning 15 April (10.3/0.4 °C) to 30 May (21.0/9.1 °C) in controlled temperature germinators. A control germination test used a constant 25 °C. The percent germination, percent viability, and average time taken to germinate were measured. The results of all three parameters corresponded in indicating which line was chilling susceptible, and the percents germination and viability in which lines were chilling tolerant. Field trials were sown in Ottawa in the early spring of 1992 and 1993 and percent of emergence and average time to emergence were recorded. The line demonstrated to be most chilling susceptible by the laboratory germination test and those lines selected to be most chilling tolerant by the laboratory percent germination and viability assays corresponded to those classified as such by the field percent emergence. Key words: Maize, germination test, chilling tolerance and sensitivity
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Ribeiro, Bárbara Gomes, Raquel Maria de Oliveira Pires, Tatiana Botelho Fantazzini, Hesoisa Oliveira dos Santos, Dayliane Bernardes de Andrade, and Renato Mendes Guimarães. "Mechanical Damages and Chemical Treatments in the Quality of Maize Seeds Stored." Journal of Agricultural Science 11, no. 3 (2019): 482. http://dx.doi.org/10.5539/jas.v11n3p482.
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The current system of seeds production is highly mechanized and these operations can contribute to losses in quality due the incidence of mechanical damages. The objective in this work was to study the effect of the chemical treatment and the storage on quality of maize seeds with mechanical damages. The seeds used were individually analyzed through X-ray test for the obtainment of three lots; 1-seeds without separation, 2-seeds without mechanical damages and 3-seeds with mechanical damages. The lots were evaluated according the physiological quality, sanity and enzymatic analyzes before and after the chemical treatment with the insecticide Maxim Advanced&reg; and the fungicide Cruiser&reg; and also after 90 days of storage. The chemical treatment of maize seeds is efficient in the control of pathogens, like Fusarium sp. The performance of seeds with internal and external damages can be maintained for 90 days when treated with fungicides and insecticides and stored in environment conditions. The isoenzymatic patterns of catalase (CAT), superoxide dismutase (SOD), malate dehydrogenase (MDH) and alcohol dehydrogenase (ADH), confirmed the higher deterioration of non-treated seeds during the storage. The mechanical damages found in seeds contribute to the reduction of viability and vigor of maize seeds.
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Teixeira, Raianny Dos Reis, and Joseanny Cardoso Da Silva Pereira. "GERMINATION AND VIGOR OF INDUSTRIALLY TREATED MAIZE SEEDS." Ipê Agronomic Journal 3, no. 1 (2019): 59–70. http://dx.doi.org/10.37951/2595-6906.2019v3i1.4331.
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The industrial seed treatment combines the application of insecticides, fungicides, nematicides, micronutrients and other products. However, seed submission to the combination of several products can cause phytotoxicity, resulting in reduced viability and vigor, which is directly proportional to the increase in the storage period of the material. Thus, the objective was to determine the vigor of corn seeds and the percentage of germination in substrates when submitted to two types of industrial treatment as a function of storage time. The experimental design was a completely randomized, 2 x 2 x 2 factorial design (two treatments, two times and two substrates / two vigor tests / two emergency tests) for the germination, vigor and emergency tests. Hybrid corn seeds were treated with two types of industrial treatments: TSI3 (combination of three products - K-Obiol 25 CE, Actellic 500 CE, Maxim Advanced) and TSI4 (combination of four products - K-Obiol 25 CE, Actellic 500 CE, Maxim Advanced, Cruiser 350 FS). The analyzed variables were: germination, vigor and emergence. In the germination test, it was verified that, when vermiculite was added, there was a higher percentage of germination in corn seeds. When added thiametoxan, the combination of products in the treatment of corn seeds had a detrimental effect on their physiological quality, reducing germination and vigor. The industrially treated corn seeds showed a reduction in vigor when submitted to the accelerated aging test after the prolongation of the storage period.
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Takele, A., and J. Farrant. "Seed germination and storage reserves of maize and sorghum after exposure to and recovery from pre- and post-flowering dehydration." Acta Agronomica Hungarica 58, no. 2 (2010): 133–42. http://dx.doi.org/10.1556/aagr.58.2010.2.4.
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Investigations were made on the seed viability (standard germination test and vigour after accelerated ageing) and seed quality (starch, protein, lipid, sucrose, glucose and fructose) of seeds of maize (cv Melkassa-2) and sorghum (cv Macia) harvested from plants after exposure to and recovery from pre- and post-flowering dehydration. The objectives of the study were to achieve a better understanding of 1) the effects of water deficit during the pre- and post-flowering stages on the seed viability and food (storage reserves) quality, and 2) the effects of dehydration and rehydration cycles at critical growth stages on subsequent seed performance and production, which could lead to the development of cultivars more able to efficiently partition assimilates to the reproductive organs in the field. The experiment was conducted in a controlled environment growth chamber under constant environmental conditions (12/12 h day/night, 28–32/17°C day/night temperature, 60–80% RH and 1200–1400 μmol m −2 s −1 PPDF). The seed viability and vigour tests were done on air-dried seeds and the seed quality analysis on freeze-dried seeds of both species.The results of the standard germination test indicated that sorghum seeds harvested after both pre- and post-flowering dehydration were not affected by the treatment, while maize seeds had reduced % germination. Sorghum seeds harvested after post-flowering dehydration had significantly decreased vigour after accelerated ageing. Dehydration during both the pre- and post-flowering stages resulted in reduced contents of protein, lipid and soluble carbohydrates (sucrose, glucose and fructose) in both species as compared to the control seeds. The species differed in the extent to which these reserves were reduced.
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Khalid, Noriza, ákos Tarnawa, Zoltán Kende, Katalin M. Kassai, and Márton Jolánkai. "Viability of maize (Zea mays L) seeds influenced by water, temperature, and salinity stress." Acta Hydrologica Slovaca 22, no. 1 (2021): 113–17. http://dx.doi.org/10.31577/ahs-2021-0022.01.0013.
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Escanferla, Maria, Philip Wysmierski, Walter Meirelles, and Luzia Meirelles. "Viability and dissemination of Pantoea ananatis, etiological agent of Maize White Spot disease." Agronomy Science and Biotechnology 4, no. 2 (2018): 52. http://dx.doi.org/10.33158/asb.2018v4i2p52.
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Maize white spot (MWS) is a disease widely spread in maize production regions in Brazil and causes serious economic damages to the culture. Little is known about the dissemination, growth and development conditions of the MWS causal agent, the bacterium Pantoea ananatis. The objective of this work was to determine the viability of this bacterium, as well as its distribution in the plant. P. ananatis after isolated was stored in both, Nutrient Broth (NB) medium and in mineral oil. For the viability tests, the cultures stored in NB were maintained at 12ºC and –6ºC and the cultures stored in mineral oil were maintained at room temperature. The isolate remained viable for seven months in NB medium at the two temperatures assessed, and four months when stored in mineral oil. The presence of the P. ananatis bacteria was analyzed in, 1) soil samples collected close to rhizosphere, 2) in seeds from plants severely attacked by MWS, 3) foliar segments of healthy plants and 4) the stem of healthy plants. P. ananatis was found surviving epiphytically on the leaf surface and its population increased with plant age, air relative humidity, and decreasing of temperature. This agent was not found neither rhizosphere nor in seeds, but it was isolated in low quantity in maize stem. P. ananatis resides epiphytically on leaves and, due to several factors, may trigger injuries to its host. All epiphytic isolates were characterized in regards to ice nucleation activity, a characteristic of this pathogen.
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Rahman, A., T. K. James, N. Grbavac, J. E. Waller, and J. Mellsop. "Spatial variability of fathen seeds in the soil under repeat maize cropping." Proceedings of the New Zealand Plant Protection Conference 52 (August 1, 1999): 209–13. http://dx.doi.org/10.30843/nzpp.1999.52.11567.
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Variability in distribution of fathen seeds in the soil was investigated to develop sampling protocols. Vertical distribution of seeds was assessed at 0-2.5, 2.5-5.0, 5.0-10.0 and 10.0-15.0 cm depth. Horizontal distribution of seeds in the top 5cm of soil was assessed in a 20 by 2.5 m grid at 50 cm intervals. Seeds were extracted from the samples by sieving and their viability was assessed. The fathen seed numbers halved with each 5 cm increase in depth. Seed numbers declined along the row in some cases but there was little localised clumping (? 1 m) of the seed. The most efficient method of sampling the fathen seedbank in a large field would be to take samples on a rectangular grid pattern to a depth of 10 cm.
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Salomão, Helis Maria, Silvia Rahe Pereira, Andreia Suchoronczek, et al. "Physiological Quality of Hybrids and Creole Maize Seeds." Ensaios e Ciência: Ciências Biológicas, Agrárias e da Saúde 23, no. 3 (2019): 198. http://dx.doi.org/10.17921/1415-6938.2019v23n3p198-202.
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AbstractThe aim of this study was to evaluate the physiological quality of hybrid maize seeds (within the validity period and expired seeds) and of a Creole variety within the validity period. The study was performed using the hybrids C1 BM3063 PRO 2 (valid until 12/2018) and C1 SYN8A98 (valid until 03/2017 - expired one year ago) and a Creole maize (freshly harvested, collected at Pato Branco city, Paraná State, Brazil). Seed germination, germination speed index, germination speed, cold test, length and fresh and dried matter of shoot and root system were evaluated in a laboratory experiment. In the field we evaluated emergency, emergency speed index, emergency speed, length and fresh and dried matter of aerial part. Creole cultivar had the worse performance in all the variables tested. Expired hybrid (SYN8A98) presented similar development to the hybrid within the validity period (BM3063) in practically all the evaluated parameters. SYN 8A98 hybrid can be sown without risks of reduced productivity, provided that cold periods during field establishment are avoided. Creole variety in question did not perform satisfactorily and could not be used as seed. Keywords: Seeds Validity Period. Open Pollinated Variety. Viability. Production Costs. Seeds Reuse. ResumoO objetivo deste estudo foi avaliar a qualidade fisiológica de sementes de milho híbrido (dentro do período de validade e sementes expiradas) e de uma variedade crioula dentro do período de validade. O estudo foi realizado utilizando os híbridos C1 BM3063 PRO 2 (válido até 12/2018) e C1 SYN8A98 (válido até 03/2017 - expirou há um ano) e um milho crioulo (recém-colhido, coletado na cidade de Pato Branco, Paraná, Brasil). Foram avaliados, em experimento laboratorial, a germinação de sementes, o índice de velocidade de germinação, a velocidade de germinação, o teste de frio, o comprimento e matéria fresca e seca da parte aérea e sistema radicular. No campo foram avaliados emergência, índice de velocidade de emergência, velocidade de emergência, comprimento e matéria fresca e seca da parte aérea. A cultivar crioula teve o pior desempenho em todas as variáveis testadas. O híbrido expirado (SYN 8A98) apresentou desenvolvimento similar ao híbrido dentro do período de validade (BM3063) em praticamente todos os parâmetros avaliados. O híbrido SYN 8A98 pode ser semeado sem riscos de produtividade reduzida, desde que sejam evitados períodos frios durante o estabelecimento em campo. A variedade crioula em questão não teve desempenho satisfatório e não pôde ser usada como semente. Palavras-chave: Período de Validade das Sementes. Variedade de Polinização Aberta. Viabilidade. Custos de Produção. Reutilização de Sementes.
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AVELING, T. A. S., V. GOVENDER, D. S. KANDOLO, and Q. KRITZINGER. "The effects of treatments with selected pesticides on viability and vigour of maize (Zea mays) seeds and seedling emergence in the presence of Fusarium graminearum." Journal of Agricultural Science 151, no. 4 (2012): 474–81. http://dx.doi.org/10.1017/s0021859612000457.
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SUMMARYThe quality of seed is dependent on two very broad aspects: how healthy (disease-free) a seed is and its field performance (germination and vigour). The objective of the present study was to evaluate the effect of pesticidal seed treatments of maize (Zea mays L.) on seed germination and vigour, and on greenhouse emergence in the presence of Fusarium graminearum Schw. Maize seeds were treated with four pesticides: Apron® XL (metalaxyl), Thiram (thiram), Celest® XL (fludioxonil, metalaxyl) and Apron® Star 42 WS (thiamethoxam, metalaxyl, difenoconazole). Viability and vigour of the treated seeds were determined. Thereafter, seeds were planted under greenhouse conditions. The control consisted of water-treated seeds. None of the pesticides reduced the standard germination under laboratory conditions and none had any effect on the quantity of leachate (measured as conductivity) or moisture content of the seeds. The different treatments also had no effect on germination or on seedling weight increase among treatments after rapid imbibition and there was no difference in germination among treatments following the cold test. The proportion of diseased plants harvested from F. graminearum inoculated soil was significantly reduced by Apron® Star 42 WS and Celest® XL. The vigour tests indicated that none of the pesticides tested affected the seeds negatively and that plant biomass in the presence of the pathogen, F. graminearum, was increased after the application of the pesticides to the seeds, with the exception of seeds treated with Apron® XL.
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Adedayo, Adetumbi, and Olakojo Adelowo. "Storage Potentials and Tolerance of High Protein Maize (HPM) and Quality Protein Maize (QPM) to Seed Storage Pests in Controlled Environment." Journal of Plant Protection Research 50, no. 1 (2010): 67–71. http://dx.doi.org/10.2478/v10045-010-0012-6.
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Storage Potentials and Tolerance of High Protein Maize (HPM) and Quality Protein Maize (QPM) to Seed Storage Pests in Controlled EnvironmentStudies on seed storage were conducted at seed processing and storage section of the Institute of Agricultural Research and Training, Obafemi Awolowo University, Moor Plantation, Ibadan Nigeria in 2007 and 2008, to evaluate High protein maize (HPM) seed for resistance/tolerance to storage insect pest, and assess the resultant effect of seed treatment chemicals on its germination potential. Seeds of HPM variety (ART-98-SW1) and Quality protein maize (QPM) variety (ILE-1-OB) and two varieties of field corn (SUWAN-1-SR and TZPB-SRW) were treated with chemicals, namely Fitscophos™, Actellic 25 EC™, Apron Star™ and combination of Actellic 25 EC and Fitscophos before storing them under controlled environment for six months. The results show that HPM, QPM and field corn require storage chemicals for effective storage, but both QPM and HPM varieties were significantly damaged by storage insect pests compared to field corn varieties regardless of the chemical used. Apron Star effectively reduced infestation by insect pests, but it may bring about significant reduction in seed viability if used for long term storage. Combination of Actellic 25 EC and Fitscophos successfully reduced infestation by insect pests without significant injurious effect on seed viability. Breeding programmes for resistance to storage pests is recommended while long term effects of Apron Star on seed viability needs to be investigated before recommending it for use in HPM/QPM maize varieties.
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Desheva, Gergana. "The Longevity of Crop Seeds Stored Under Long-term Condition in the National Gene Bank of Bulgaria." Agriculture (Polnohospodárstvo) 62, no. 3 (2016): 90–100. http://dx.doi.org/10.1515/agri-2016-0010.
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Abstract Seed accessions from 7 plant families and 28 species stored for above 20 years in the National gene bank of Bulgaria were evaluated. All seed accessions were maintained as base collection under long-term storage conditions with low moisture contents (5±2%) in hermetically closed containers at −18°C. On the basis of experimental data, the seed storage characters σ (standard deviation of seed death in storage), P50% (the time for viability to fall to 50%) and P10% (the time for viability reduction of 10%) were determined allowing the prediction of seed storage life and the regeneration needs. The results showed significant differences in loss of seed viability among species and within the species. After 20–24 years of storage, eleven crops showed minimal viability decline under 5% as compared to the initial viability (oats, barley, maize, bread wheat, durum wheat, smooth brome grass, faba bean, chickpea, sunflower, cucumber and pepper). For the same storage time, another group of crops (sorghum, triticale, orchard grass, tall fescue, common vetch, grass pea, lentil, common bean, rapeseed, tobacco, flax, cabbage and tomatoes) presented 5–10% reduction of seed viability. More significant changes in seed viability – above 10% – were detected for peanuts, lettuce, soybean and rye. The σ values varied from 20.41 years (Arachis hypogaea L.) to 500 years (for Avena sativa L. and Triticum aestivum L). There was wide variation across species, both in time taken for the viability to fall to 50% and in time taken for the seed viability reduction of 10%. The study illustrates the positive effect of both seed storability early monitoring and prediction of regeneration needs as a tool for limiting undesired losses.
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Muthukumar, M., and K. N. Ragumoorthi. "Impact of Angoumois Grain Moth, Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae) on the Viability of Maize Seeds." International Journal of Current Microbiology and Applied Sciences 6, no. 8 (2017): 5–10. http://dx.doi.org/10.20546/ijcmas.2017.608.002.
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Vázquez, Edgar, Fernando Montiel, and Jorge M. Vázquez-Ramos. "DNA ligase activity in deteriorated maize embryo axes during germination: a model relating defects in DNA metabolism in seeds to loss of germinability." Seed Science Research 1, no. 4 (1991): 269–73. http://dx.doi.org/10.1017/s0960258500000994.
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AbstractChromosome integrity and DNA polymerase activity are negatively affected by improper storage of maize seeds. We have extended our studies on the effect of improper storage on DNA metabolism by analysing the effect of hot–dry and hot–humid conditions on DNA ligase activity. An assay to measure ligase activity was established by using linearized plasmid DNA as substrate and protein extracts from maize axes. Optimal conditions were established for the in vitro assay. Enzyme activity was importantly reduced under both storage conditions. Activity recovered 6–9 h after imbibition. A model for loss of viability and vigour due to damage of DNA metabolism is proposed.
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Marinoni, Renato C., and Cibele S. Ribeiro-Costa. "Influence of temperature and diet on the development of Ulomoides dermestoides (Fairmaire, 1893) (Coleoptera, Tenebrionidae, Diaperinae)." Brazilian Archives of Biology and Technology 44, no. 2 (2001): 129–34. http://dx.doi.org/10.1590/s1516-89132001000200004.
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Ulomoides dermestoides (Fairmaire, 1893) develops in stored food products (peanuts, maize, oats, rice, sorghum, etc.) and breeds successfully in the laboratory. To determine the best conditions for development, experiments were set up in different temperatures and diets, similar to storage conditions of peanuts (Arachis hypogaea L.). The higher viability of individuals and the shorter developmental time were observed in the diet composed of hulls and seeds of fruits at 21 and 24°C.
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GHASSEMI-GOLEZANI, Kazem, Bahareh DALIL, Mohammad MOGHADDAM, and Yaeghoob RAEY. "Field Performance of Differentially Deteriorated Seed Lots of Maize (Zea mays) under Different Irrigation Treatments." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39, no. 2 (2011): 160. http://dx.doi.org/10.15835/nbha3926055.
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A sub-sample of maize (cv. KSC301) seeds was kept as control or vigorous seed lot and two other sub-samples with about 16% moisture content were artificially deteriorated at 40C for 16 and 18 days. The three seed lots had seed viabilities of 99% (V1), 91% (V2) and 79% (V3). Laboratory tests were carried out as CR design with four replicates. However, the field experiment was conducted as factorial based on RCB design with three replicates to investigate the performance of differentially deteriorated seed lots under four irrigation treatments (irrigation after 70, 90, 110 and 130 mm evaporation from class A pan). Germination and emergence times significantly increased with increasing seed deterioration, but mean viability percentage, seedling dry weight, seedling emergence and protein and grain yields decreased as seed deterioration increased. Even plants from less deteriorated seed lot (V2) with acceptable germination (91%) showed 25.1% reduction in grain yield per unit area. The superiority of V1 to V2 seeds of maize increased as water limitation increased. Protein yield was also decreased as a result of seed deterioration. Seedling dry weight highly correlated with the field emergence and grain and protein yields. Thus, it is necessary to produce and cultivate high vigor seeds of maize, in order to ensure satisfactory yield achievement, particularly under adverse environmental conditions.
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Davidson, K. G. V., F. D. Moore, and E. E. Roos. "299 ELECTROLYTE LEAKAGE FROM INDIVIDUAL MAIZE AND WHEAT SEEDS TO PREDICT GERMINATION PERCENTAGE AND RADICLE LENGTH." HortScience 29, no. 5 (1994): 473b—473. http://dx.doi.org/10.21273/hortsci.29.5.473b.
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Multiple electroconductivity readings (μAmps) of leachates from individual seeds during the first 6 hr of imbibition was examined as a possible predictor of seed quality. Readings from each sample of 100 seeds were formed into frequency distributions and the mean, median. and internal slope were calculated using an automated computer retrieval system. Internal slope is a non-central tendency measure based on the slope of a line tangent to the inflection point of the S-shaped cumulative frequency distribution (CFD) of μAmps. Radicle lengths (3 days) and germination (7 days) percentages were regressed on each of the two measures of central tendency and also the CFD shape indicator, internal slope. None of the three predictors were satisfactory for estimating seed vigor (root lengths) of maize (Zea mays L.) or wheat (Triticum aestivum L.) or germination of wheat seed lots. Internal slope was useful in estimating viability of artificially aged maize seeds (r2= 0.91), which compared favorably with our previous results using naturally aged red clover (Trifolium pratense L.) seed lots. Overall the seed quality of the unaged wheat lots was fairly high and the electrolyte leakage test was not sensitive enough to detect differences within these lots.
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Tonel, Fernanda Reolon, Patrícia Marini, Juliana de Magalhães Bandeira, Dario Munt de Moraes, and Luciano do Amarante. "Salicylic acid: physiological and biochemical changes in seeds and maize seedlings subjected to salt stress." Journal of Seed Science 35, no. 4 (2013): 457–65. http://dx.doi.org/10.1590/s2317-15372013000400007.
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Several plant species have development limitations on saline soils; however under water stress situations salicylic acid (SA) may have defense function by acting as antioxidant. Thereby, this study was aimed at analyzing physiological changes occurring in maize seeds treated with SA and germinated on sodium chloride moistened substrate; as well as assessing the effect of antioxidant enzymes produced by the seedlings. To this, seeds were soaked into zero and 1 mM SA solutions, for 60 min., and germinated on different NaCl concentrations (0, 30, 90, and 180 mM), and afterwards evaluated for germination, germination speed index, initial growth, and effect of antioxidant enzymes on seedlings. For electrical conductivity test, the seeds were previously soaked in NaCl solutions with and without SA. The SA application has minimized damages induced by salt stress to maize seed germination by favoring their germination percent; however without SA was not able on maintaining seed viability over 90 mM. Seedling initial growth was impaired by increasing NaCl concentrations in substrate; and electric conductivity has shown low values for release of solutes in the presence of SA. Activity of antioxidant enzymes has increased until 30 mM NaCl concentration; however above such concentration activity of these enzymes was reduced. The SA maintains germination of corn seeds above 90 mM NaCl; however, generally it is not efficient to ensure vigor of seedlings.
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Wambugu, P. W., P. W. Mathenge, E. O. Auma, and H. A. vanRheenen. "Constraints to On-Farm Maize (Zea mays L.) Seed Production in Western Kenya: Plant Growth and Yield." ISRN Agronomy 2012 (August 2, 2012): 1–7. http://dx.doi.org/10.5402/2012/153412.
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Studies have shown that that about 85% of maize farmers in Western Kenya plant local varieties with about 80% using own farm-saved seeds. The production system is characterized by late harvesting, heavy striga infestation, use of local varieties, and low-soil fertility. The objective of this study was to test an on-farm seed production system which would help improve yield and quality of farm saved seeds. The trials were set up in a factorial design fitted as random complete block design. There were 3 factors each at 2 levels: time of harvest, variety choice, and fertilizer application. Fertilizer application led to an 88% increase in yield, 54% increase in number of seeds per cob, and 14% increase in 100-seed weight. Fertilizer application also led to an increase in seed vigour and viability. Yield differences between the 2 varieties were not significant. The correlation between 100-seed weight and seed vigour was significant showing that heavy seeds were more vigourous. Nitrogen application was therefore recommended for increasing yields and for producing vigourous seeds but should be done with caution to avoid lodging as witnessed. This study also noted that farmers are rational and their decisions are usually based on strong economic considerations.
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Guzzon, Filippo, Maraeva Gianella, Jose Alejandro Velazquez Juarez, Cesar Sanchez Cano, and Denise E. Costich. "Seed longevity of maize conserved under germplasm bank conditions for up to 60 years." Annals of Botany 127, no. 6 (2021): 775–85. http://dx.doi.org/10.1093/aob/mcab009.
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Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.
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Guzzon, Filippo, Pedro Bello, Kent J. Bradford, María de los Angeles Mérida Guzman, and Denise E. Costich. "Enhancing seed conservation in rural communities of Guatemala by implementing the dry chain concept." Biodiversity and Conservation 29, no. 14 (2020): 3997–4017. http://dx.doi.org/10.1007/s10531-020-02059-6.
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Abstract Seed conservation in rural communities of low- and middle-income countries located in tropical areas is particularly problematic, due to high relative humidity that promotes insect and fungal infestations and leads to rapid losses in seed viability. Seed conservation in those areas is affected by unreliable power supplies that do not allow the use of dehumidifying and refrigeration systems recommended for the long-term storage of seeds. We tested the dry chain, i.e., initial seed drying with a reusable desiccant in the form of zeolite beads followed by seed conservation in hermetic containers, in rural communities of Guatemala (Huehuetenango Department). In this region, a network of community seed reserves (CSRs) has been established to provide a safety backup for seed and to conserve local agrobiodiversity. Using a local maize variety in three communities, we compared the dry chain with the seed conservation methodology employed in the CSRs (i.e., undried seeds in hermetic flasks) as well as with seed conservation in open storage, both in the local CSR and in a farmer’s granary. Seed conserved using the dry chain treatment maintained very high seed viability (> 80%) throughout the whole experiment (6 months) and reduced fungal and insect infestations (< 3%). In the other treatments, the viability declined significantly to an average of 52% non-viable and 19% infested seeds after 6 months of storage. The dry chain was demonstrated to be an excellent solution for enhancing seed conservation in biodiversity hotspots of tropical areas as well as for improving seed security for farmers.
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Yan, Min. "The preliminary study on the optimum moisture content of ultra-dry storage and its related chemicals in seeds from six crop species." Plant Genetic Resources 15, no. 6 (2016): 506–14. http://dx.doi.org/10.1017/s1479262116000216.
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AbstractUltra-dry storage, storing orthodox seeds at ambient temperatures with moisture contents below 5%, can maintain seed viability for long periods with low input. Optimum moisture contents for ultra-dry storage exist and vary substantially among different seed lots due to their differences in desiccation tolerance, which is associated with the accumulation of protective compounds. To study the relationship between moisture contents of ultra-dry stored species, seed composition and the free-radical scavenging activity (FRSA). Eleven seed lots of maize, wheat, pepper, peanut, Chinese cabbage, and cucumber were chosen and multiple stepwise regression analysis was applied. In general, analysis of the seed lots revealed that optimum moisture contents for highest seed viability are below equilibrium moisture at 13% relative humidity and 20°C. Nevertheless, seed composition differed significantly among species. Lipids, flavonoids and the FRSA were significantly negatively correlated with the optimum moisture content of ultra-dry stored whole seeds and accounted for 97.7% of the total variation in the optimum moisture content. Lipids had the highest effect (90.0%) on the optimum moisture content of ultra-dry storage, which is consistent with the previous results. The FRSA and flavonoids accounted for 4.8 and 2.9% of total variation of optimum moisture content of ultra-dry stored whole seeds, respectively. The FRSA and flavonoids were also significantly negatively correlated with the optimum moisture content for non-lipid fraction of ultra-dry stored seeds, and accounted for 74.1 and 15.3% of the total variation in the optimum moisture content, respectively.
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Harman, Rebecca Mattingly, Neal S. Eash, John E. Morrison, William E. Hart, Casey T. Sullivan, and Dayton M. Lambert. "Finding the Technological Sweet Spot: The Smallholder Conservation Agriculture Maize Seeder." Journal of Sustainable Development 10, no. 5 (2017): 241. http://dx.doi.org/10.5539/jsd.v10n5p241.
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The seeder is integral to smallholder agricultural production. This technology seeks to lessen farmer labor requirements, meter seeds accurately, and minimize excessive soil disturbance. Hand seeders play a central role in conservation agriculture (CA) for the smallholder farmer as a means to plant through residue cover and penetrate non-tilled soil surfaces. Two trials in maize (Zea mays, L.) residue and soybean (Glycine max, L.) residue were conducted to test seven seeders of increasing mechanization levels: five hand operated, one mechanized, and one tractor-drawn control. The experiment site was in Mt. Gilead, Ohio, at the Eastern end of the US “Corn Belt” that had been under continuous no-till production for seven years. Experimental conditions at the site sought to mimic smallholder conditions through seeding and hand harvesting. Seeders were evaluated based on plant population establishment, crop growth stage, crop heights and final maize grain yield. The hand seeders were further evaluated based on their economic viability and usability – key challenges to the ultimate adoption of new seeding technologies. The study found that the seeders tested performed equally to the control, the John Deere MaxEmerge Conservation planter, the crop-seeding capacity and price evaluation identified the Haraka rolling planter ill-suited for smallholders while the OSU Greenseeder proved highest qualitative performance. In conclusion, all evaluations indicate that a medium level of mechanization is appropriate and necessary to be successful in a smallholder CA system although continued research is necessary.
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Blackman, Sheila A., and Eric E. Roos. "441 PB 244 CULTURE OF ISOLATED EMBRYOS OF DETERIORATED MAIZE SEED: A STRATEGY FOR RESCUING GERMPLASM." HortScience 29, no. 5 (1994): 494d—494. http://dx.doi.org/10.21273/hortsci.29.5.494d.
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The low quality of some seed lots received by germplasm repositories such as the National Seed Storage Laboratory can thwart efforts to regenerate seed for storage. This germplasm is in danger of irretrievable loss. The aim of this work is to promote the germination, and hence regeneration, of such low quality seeds through sterile culture of the isolated embryos. Hybrid (B73×LH51) maize seeds were aged 5 y at 32°C and 0.037 g H2O g-1 dry wt. Vigor - but not viability -declined under these conditions. The effects of four factors on growth and germination were systematically examined. These were: seed pretreatments; antibiotics and fungicides; nutrients; and growth substances. Amongst the pretreatments, none surpassed partial hydration of seeds for 24 hr to 0.55 g H2O g-1 dry wt at 25°C prior to embryo dissection. Thiram (2.4 mg mL-1) and kanamycin (50 ug ml1) effectively controlled bacterial and fungal growth with no deleterious effects on growth during culture of the isolated embryos. Exogenous sucrose (optimum 5 % wt/vol) significantly stimulated radicle growth in both deteriorated and non-deteriorated embryos. No other organic or inorganic nutrient stimulated growth. Naphthalene acetic acid did not affect growth while kinetin reduced radicle growth and stimulated coleoptile growth. Gibberellic acid (GA3 at 10-5M) significantly stimulated radicle growth in deteriorated embryos, whereas it promoted coleoptile growth in both deteriorated and non-deteriorated embryos. These data suggest GA or a GA-stimulated process may limit the growth of aged embryos.
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Valdes, Abel, Elos Mariano Mendoza, and Francisco Nieto. "Control de Prostephanus truncatus (Horn) en semilla de maíz almacenada." Agronomía Mesoamericana 11, no. 1 (2006): 95. http://dx.doi.org/10.15517/am.v11i1.17358.
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The objective considers to evaluate the biological effectiveness of 9 single insecticide products and in mixture, employees for the control of plagues of the maize (grain stored and of the culture) and to dose recommended for this culture. They were applied by immersion of the grain on a variety of maize tuxpeño to observe the biological effect in mortality terms on large grain borer Prostephanus truncatus, during a period of storage of 6 months, under a design completely at random with 38 treatments, 3 repetitions and one comparison of averages (Tukey 0,05); with the intention of analyzing the insecticide that presents the best option for the treatment to the maize seed stored for investigation. The variance analysis indicated significant differences in percentage of mortality of the insects for each one of the treatments. The effect insecticides was of 100% to 5.53% of mortality. The insecticides deltametrina, permetrina and carbaryl expressed greater percentage of mortality on the others, including the witness (0% mortality). The mixtures in which good result was had are: deltametrina + pirimifos metil, deltametrina + carbaryl, permetrina + carbaryl, carbaryl + pirimifos metil, carbaryl + clorpirifos etil. The test of germination of seeds at the end of the evaluation showed, that the viability of the same one was not affected by the treatments.
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Henrique, Ivanildo Guilherme, Rafael Orlandini Bosqueiro, Rafael Massaru Kotsubo, and Silvia de Carvalho Campos Botelho. "DÉFICIT HÍDRICO E A GERMINAÇÃO DE SEMENTES DE HÍBRIDOS DE MILHO." Nativa 9, no. 3 (2021): 240–46. http://dx.doi.org/10.31413/nativa.v9i3.9686.
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Considerando a importância da água no processo de germinação de sementes, as condições adversas de umidade afetam diretamente o processo germinativo. Para tanto, objetivou-se avaliar a influência do deficit hídrico na germinação e vigor de sementes de dois genótipos comerciais de milho, induzido por diferentes potenciais osmóticos. Utilizou-se delineamento experimental inteiramente casualizados (DIC), arranjado em esquema fatorial duplo 2x5, com quatro repetições, avaliando híbridos de milho (DKB255PRO3 e DKB390PRO2), submetidos a germinação sob condições de déficit hídrico, em cinco níveis de potencial osmótico (0, -0,6, -1,2, -1,8 e -2,4 MPa). Cada unidade experimental constituiu-se por um rolo de papel germitest contendo 50 sementes. Utilizou-se NaCl como agente osmótico indutor de deficiência hídrica as sementes. Avaliou-se a germinação, vigor de sementes e o desenvolvimento inicial das plântulas, em termos de comprimento e biomassa. O déficit hídrico, simulado com solução de cloreto de sódio, provoca redução no desempenho de sementes de híbridos de milho. Os genótipos de milho respondem diferentemente quanto a tolerância a condições de estresse provocado por indisponibilidade de água durante a germinação. O híbrido DKB390PRO2 apresenta germinação e crescimento de plântulas superior quando submetido a diferentes níveis de potencial osmótico de NaCl. Palavras-chave: viabilidade de sementes; restrição hídrica; Zea mays. Effect of water deficit on the maize seed germination ABSTRACT: Considering the importance of water in the seed germination process and knowing that conditions will not always be the most adequate to the germination process, the objective was to evaluate the influence of water deficit on germination and vigor in seeds of different commercial corn genotypes induced by different osmotic potentials. A completely randomized experimental design (D.I.C) was used, arranged in a 2x5 double factorial scheme, with four replications. Each experimental unit was constituted by a roll containing 50 seeds, made in germitest paper. Two maize seed hybrids (DKB255PRO3 and DKB390PRO2) were evaluated, submitted to germination under water deficit conditions, with levels 0; -0,6. -1,2; -1,8 and -2,4 MPa of osmotic potential. NaCl was used as osmotic agent inducing water deficiency in seeds. Germination, seed vigor and initial seedling development were evaluated in terms of length and biomass. The water deficit simulated with sodium chloride solution, causes a reduction in the performance of maize hybrids seeds. Corn genotypes respond differently to tolerance to stress conditions caused by unavailability of water during germination. The hybrid DKB390PRO2 presents superior germination and seedling growth when submitted to different levels of osmotic NaCl potential. Keywords: viability; water restriction; Zea mays.
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WARWICK, S. I., and L. D. BLACK. "THE BIOLOGY OF CANADIAN WEEDS.: 90. Abutilon theophrasti." Canadian Journal of Plant Science 68, no. 4 (1988): 1069–85. http://dx.doi.org/10.4141/cjps88-127.
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Abutilon theophrasti Medic. (velvetleaf) forms extensive weed infestations in all major maize and soybean growing areas of Ontario and Quebec. A review of the literature on the biology of the species is presented. Velvetleaf causes crop losses through competition, allelopathic effects and by hosting insect pests and pathogens of crops. Velvetleaf has a number of features which contribute to its success as a weed, including: the production of a large number of seeds that have high viability with prolonged dormancy and sporadic, continuous germination patterns; robust seedling vigor; and the ability to produce seed under competition. Because of sporadic germination patterns, control is difficult. Effective control measures include the application of pre-emergence and/or postemergence herbicides followed by cultivation and additional herbicide applications to control escapes and later flushes of germination. A triazine-resistant bio-type of velvetleaf has recently been reported from the northeastern United States.Key words: Velvetleaf, Abutilon theophrasti, weed biology
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Khatun, Monowara, Md Asif Rehan Shuvo, Md Tareq Bin Salam, and S. M. Hafizur Rahman. "Effect of organic amendments on soil salinity and the growth of maize (Zea mays L.)." Plant Science Today 6, no. 2 (2019): 106–11. http://dx.doi.org/10.14719/pst.2019.6.2.491.
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Soil salinity is a major concern in southwestern part of Bangladesh because almost 30% cultivable lands are currently lying under risk of salinity where 30-50% yields loss is happening. Organic amendments have found to be effective in the amelioration of saline soil by improving soil physical and chemical properties as well as crop selection is another criteria for sustaining viability of crops in saline soil. For ensuring sustainable saline soil management, a comparative pot study was carried out during kharif 1 season in 2015 to observe the effect of organic amendments (solid waste, vermicompost and cow dung) on soil salinity and its influence on the growth of maize. Composite soil was collected at a depth of 0-15 cm from Gozalmari village of Jalma Union in Batiaghata Upazila under Khulna district, Bangladesh that was saline (10.6 dS/m) in nature and the irrigation water sample was collected from beside Kazibacha river (4.28 dS/m) that was also moderately saline. The maize cultivar “Shuvra” was used for cultivation in the study. The experiment comprised of four treatments viz. T0: Control (No organic manure); T1: Solid waste (36g); T2: Vemicompost (72g); T3: Cow dung (33g). Five seeds were sown in each pot. Seeds were treated with Agrosan GN to protect them from seed and soil borne pathogens. Chemical fertilizers were not used in the experiment. Irrigation was done two times before harvesting: at 20 days after sowing (DAS) and at 40 DAS with river water and rain water was irrigated naturally during the season. Findings were that the organic amendments significantly influence the physico-chemical properties of the saline soil. All organic treated soils significantly reduce the soil EC (from 10.6 dS/m to 3.4 dS/m) and pH (from 7.63 to 7.38) compared to control soil (p?0.05). In case of survival parameters (e.g %gemination, rate of survival at 50 DAS) of maize, the treatments were found insignificant (p?0.05). But in terms of growth parameter (plant height and root length), significant differences were found between control and organic amendments treated soil (p?0.05). It may be concluded that organic amendments treated soils showed better results than that of control soil. If proper management can be implemented, this positive results will bring hope to the local poor farmers at least can introduce a new crop in fallow agricultural land during the kharif 1 season.
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Komisarenko, A. G. "Osmotolerance of т4 generation monocotyledonous and dicotyledonous plants with suppressed expression of proline catabolism gene". Fiziologia rastenij i genetika 52, № 5 (2020): 434–48. http://dx.doi.org/10.15407/frg2020.05.434.
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Analysis of transgene function retention in seed generation (Т4) of genetically modified plants of maize, wheat and sunflower was carried out. It was shown that about 80–85 % of seeds of plants created by the biotechnological method had the ability to germinate under conditions of water deficiency and salinization, while in plants of the original forms this index was 20—28 %. Variability in transgene expression among individual variants of the seed generation of genetically modified forms was noted. Almost 65 % of Т4 seedlings of corn and wheat withstood the conditions of super hard osmotic stress created by adding 0,8 M manite to the culture medium, which was lethal to the original forms. Progenies of transgenic plants were also characterized by an increased level of resistance to drought created by the water cessation, which was manifested in the indices of growth processes. At the stage of restoration after the action of prolonged dehydration, the biotechnological plants of sunflower were 17 cm higher than the original form and had 1.5 times larger biomass. Maintaining the viability of genetically modified plants under hard stressful conditions was associated with an increase in the level of free L-proline (Pro). Genetically modified plants had 1.5—2 times higher Pro content compared to the original form both under normal moisture supply and under its deficiency, which may result from partial suppression of the proline dehydrogenase (PDH, pdh) gene. It was found that under normal growth conditions, the activity of the PDH enzyme in transgenic Т4 maize and sunflower seedlings was almost 3 times lower than in the original forms, while for wheat, this difference was 1.6 times. The tendency to lower relatively to control PDH activity in the Т4 generation of the studied representatives of transgenic monocotyledonous and dicotyledonous plants was observed at all stages of growth.
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Kim, Park, Kang та ін. "Green Tea Seed Oil Suppressed Aβ1–42-Induced Behavioral and Cognitive Deficit via the Aβ-Related Akt Pathway". International Journal of Molecular Sciences 20, № 8 (2019): 1865. http://dx.doi.org/10.3390/ijms20081865.
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The aim of this study was to investigate the availability of seeds, one of the byproducts of green tea, and evaluate the physiological activity of seed oil. The ameliorating effect of green tea seed oil (GTO) was evaluated on H2O2-induced PC12 cells and amyloid beta (Aβ)1–42-induced ICR mice. GTO showed improvement of cell viability and reduced reactive oxygen species (ROS) production in H2O2-induced PC12 cells by conducting the 2′,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 2′,7′-dichlorofluorescein diacetate (DCF-DA) analysis. Also, administration of GTO (50 and 100 mg/kg body weight) presented protective effects on behavioral and memory dysfunction by conducting Y-maze, passive avoidance, and Morris water maze tests in Aβ-induced ICR mice. GTO protected the antioxidant system by reducing malondialdehyde (MDA) levels, and by increasing superoxide dismutase (SOD) and reducing glutathione (GSH) contents. It significantly regulated the cholinergic system of acetylcholine (ACh) contents, acetylcholinesterase (AChE) activities, and AChE expression. Also, mitochondrial function was improved through the reduced production of ROS and damage of mitochondrial membrane potential (MMP) by regulating the Aβ-related c-Jun N-terminal kinase (JNK)/protein kinase B (Akt) and Akt/apoptosis pathways. This study suggested that GTO may have an ameliorating effect on cognitive dysfunction and neurotoxicity through various physiological activities.
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Martin, H. L. "Management of soilborne diseases of beetroot in Australia: a review." Australian Journal of Experimental Agriculture 43, no. 11 (2003): 1281. http://dx.doi.org/10.1071/ea02150.
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Soilborne fungal diseases threaten the viability of the Australian processing beetroot industry. Globally, Pythium spp., Aphanomyces cochlioides and Rhizoctonia solani are the predominant soilborne fungal pathogens responsible for a root rot complex in beet crops. In Australia, the disease problems have been exacerbated in recent years because crops are now grown virtually year round, and under environmental conditions favourable to infection. This has lead to increased inoculum levels in soils sown to beetroot. Moreover, nowhere in the world does there seem to be a single strategy that is completely efficacious in controlling these pathogens, so an effective management strategy will almost certainly involve a combination of tactics. The most likely combination seems to involve fungicide seed treatments, rotations of gramineous or biofumigant crops and the use of disease-resistant varieties of beet. The first of these, fungicidal seed dressings, are a cost-effective means of reducing pathogen inoculum and protecting young beets from infection. Hymexazol may be particularly useful in the Australian system, since it is active against both Pythium and Aphanomyces. To combat Rhizoctonia, it should be applied in combination with either pencycuron or toclophos methyl. Second, rotations of gramineous crops, such as maize or oats, or biofumigant brassica crops, such as white mustard, brussel sprouts or cabbage should also reduce the build-up of inoculum and offers a tactic to relieve the problem in the longer term. Third, alternative beetroot cultivars with resistance to Rhizoctonia have now been developed in USA breeding programs and should be considered by the Australian industry. Resistant varieties are potentially very useful since they offer a long-term approach to disease management that can be easily incorporated into existing production systems. Fourth, the alteration of sowing dates to avoid periods of high disease risk (e.g. confining sowing to the cooler, drier months) needs to be seriously considered by the Australian industry if it is committed to disease management. There is also evidence to suggest that seed priming may warrant consideration as a tool to use in combination with fungicide dressings to reduce disease in young plants. Biological seed treatments and soil fumigation appear to be tactics of limited value to the Australian beet industry.
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Hahn, Juliane, Friederike de Mol, and Jürgen Müller. "Ensiling Reduces Seed Viability: Implications for Weed Management." Frontiers in Agronomy 3 (August 5, 2021). http://dx.doi.org/10.3389/fagro.2021.708851.
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Ensiling, a lactic acid fermentation process, is mainly used to preserve biomass. In addition, it has been shown to affect seed viability of some plant species. The extent to which this makes ensiling suitable as a weed control measure, however, has not yet been determined. Both the range of controllable species and the parameters of an ensiling process that safely kills seeds are still undefined. We aimed to determine the effect of varying substrate and ensiling conditions on the seed viability of 10 species selected to represent a wide range of different seed traits. Five different types of silages were made from maize or mixtures of wildflower and maize biomass and ensiled in lab-scale silos for 8 months. The pure maize silages were prepared under conditions either ideal or suboptimal for ensiling forage. Seeds of important weeds (Chenopodium album, Abutilon theophrasti) and of species from a wildflower mixture suitable for ensiling and biogas production (Cichorium intybus, Daucus carota, Echium vulgare, Malva alcea, Malva sylvestris, Melilotus albus, Melilotus officinalis) were tested. Seed viability was determined using a combination of tetrazolium and germination tests. Ensiling reduced seed viability across all 10 species significantly. Seed-killing efficacies of ensiling, however, differed widely among the species studied, largely related to whether the species could produce hard (physically dormant) seeds. Seeds from species without hardseededness were completely inactivated by ensiling, while the seed-killing efficacies for hardseeded species ranged from 5 to 60%. Variation in ensiled substrate and ensiling conditions had no consistent effect on seed survival. We concluded that ensiling has the potential to sustainably reduce seed viability of a wide range of species and therefore should be adopted as a component of integrated weed management in organic agriculture.
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Lesilolo, M. K., Jacob Patty, and N. Tetty. "Penggunaan Desikan Abu Dan Lama Simpan Terhadap Kualitas Benih Jagung (Zea mays L.) Pada Penyimpanan Ruang Terbuka." Agrologia 1, no. 1 (2018). http://dx.doi.org/10.30598/a.v1i1.298.
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This study was aimed to determine the proper dose of ash desiccant on maize seed stored for 30, 60 and 90 days. The design used was a Completely Randomized Factorial Design, with two factors and three replications. The first factor was desiccant dose (D) : Do = 0 % (control), D1 = 5 % (12.5 g of seed weight), D2 = 10% (25 g of seed weight), D3 = 15 % (37 g of seed weight), D4 = 20 % (50 %g of seed weight), D5 = 25 % (62.5 g of seed weight). The second factor was length of storage (S) : S1 = 30 day storage, S2 = 60 day storage and S3 = 90 day storage. The result show that use of desiccant only gave effect on the water content of seeds (12.31 %) and speed of seed growth vigor parameters (24.84 %). Use of desiccant in 90 day storage were able to maintain water content of seeds (12.43 %), seed germination viability parameter (100 %), and vigor of seeds, which consisted of seed growth simultaneity (66.77 %) and seed growth speed (28.71 %). The interaction between the use off ash desiccant and the length of storage had no effect on viability and vigor of maize (Zea mays L) seeds but had an effect on their water content after storage (12.73%).
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Olawuyi, Odunayo Joseph, Onyinye Mirian Chikeluba, and Akinlolu Olalekan Akanmu. "Effect of Storage Temperatures on Phenotypic and Gene Expression of Maize (Zea mays L.) Genotypes." Biotechnology Journal International, March 19, 2020, 38–52. http://dx.doi.org/10.9734/bji/2020/v24i130096.
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Introduction: Maize is an important cereal grown globally across wide range of altitude and latitude. Temperature is one of the factors that affect the viability of maize under storage conditions.
 Aim: This study, therefore, assessed the effect of different temperature storage levels on the morphological characters and molecular variability of maize genotypes.
 Materials and Methods: The seeds of maize genotypes: TZLCOMP4C3, EVDT-W200STRCO, POP66SR/ACR94, POOL18SR QPM, TZM 132, TZM 1291, EVDT- Y2008 STR and TZM 1326 obtained from the National Centre for Genetic Resources and Biotechnology (NACGRAB) Ibadan were stored at different temperature conditions of -80°C, -20°C, 5°C, 50°C, and 25°C for 6 hours. The stored maize was sown in perforated polythene bags containing 7 kg of soil and replicated thrice in a complete randomized design. The molecular variability was also investigated on the maize genotypes stored under the varying temperatures. The effect of storage temperature was significantly higher at 50°C for all growth characters but was not significant at -80°C.
 Results: The genotypic effect on the growth characters was significantly (p< 0.05) higher in TZM 132 with plant height (99.09 cm), leaf length (46.54 cm), leaf width (3.05 cm), number of leaves (6.25) and stem length (50.12 cm). The contribution of principal component axis (PCA) showed that PC 1 had the height variation with a proportion of 47.17% and eigen value of 2.83 across the growth characters. Molecular evaluation showed that EVDT-W200STRCO had the highest DNA concentration of 4885.7 ng/μl at storage temperature of 25°C, while EVDT-W200STCO at -20°C recorded the least DNA concentration of 26.60 ng/μl. The highest DNA concentration across the maize varieties were recorded at -20°C (POOL18SR QPM, TZM 132 and TZM 1326), 5°C (TZLCOMP4C3), 25°C (EVDT-W200STRCO and EVDT- Y2008 STR) and 50°C (POP66SR/ACR94 and TZM 1291). OPB 10 had the highest allelic no, gene diversity and polymorphic information content of 15, 0.97 and 97.0% respectively. The genetic distance matrix established relationship among the stored maize genotypes.
 Conclusion: Maize seeds can therefore be stored at temperature range of -20°C to 50°C depending on the variety, without losing its viability and molecular constituents.
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Bankole, O., A. Oluwaranti, S. A. Ajayi, and F. E. Awosanmi. "Genotypic Variation of Different Maturity Groups of Maize (Zea mays L.) for Seed Physiological Quality." Journal of Experimental Agriculture International, June 27, 2020, 139–49. http://dx.doi.org/10.9734/jeai/2020/v42i530527.
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Maize genotypes of different maturity groups with good seed quality had been developed, but there is little or no information on how the seed quality of the maize genotypes are affected by their maturity groups. Therefore, the objectives of this study were to evaluate maize varieties of different maturity groups for seed quality and determine genotypic variations that exist within the different maturity groups of maize for seed quality. Laboratory experiments were conducted in the Seed Science Laboratory of the Department of Crop Production and Protection, Obafemi Awolowo University, Ile-Ife, Nigeria. Seeds of sixteen maize genotypes of different maturity groups were subjected to imbibition, germination, accelerated ageing, conductivity and seedling vigor tests. The late maturity group (TZLCOMP.4DTF2,TZLCOMP. 1C6/DT.SYN-1-W, WHITEDTSTR/TZLCOMP.1-W, OBASUPER1) gave the best viability, speed of germination and days to complete germination while the extra early maturity group (2009TZEE-ORISTRQPM, 2000SYNEE-WSTR,2013TZEE-WDTSTR, TZEE-WPOPDTC2STRC5F2) had the least performance. The early (2014TZE –YDTSTR, 2013DTESTR–WSYN, EVDT-2000STRC0, 2009DTEWSTRSYN) and extra-early maturity groups had the longest root length and highest root number respectively. However, the different maturity groups of maize evaluated imbibed water at the same rate. TZEE-WPOP DT STR C5 F2, 2013 DTE STR – W SYN, AFLATOXIN R-SYN2, TZL COMP.1 C6/DT. SYN-1-W among extra early, early, intermediate and late maturity groups respectively performed best for viability and vigor tests.
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de Brito, R. R., E. F. Leão-Araújo, L. B. da S. Ferreira, et al. "Research Article Viability of simultaneous genotypic screening and germination of individual seeds in rice, beans and maize." Genetics and Molecular Research 18, no. 1 (2019). http://dx.doi.org/10.4238/gmr18130.
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SILVA, THAYS GABRIELLA LIMA, ROMANO ROBERTO VALICHESKI, FLÁVIO LOPES CLÁUDIO, ESTÊNIO MOREIRA ALVES, MATEUS DE SOUSA PERES, and PAULO ALEXANDRE PERDOMO SALVIANO. "SILAGE PRODUCTION, BROMATOLOGICAL COMPOSITION AND ECONOMIC VIABILITY OF INOCULATION OF VARIETAL MAIZES WITH." Revista Brasileira de Milho e Sorgo 20 (September 3, 2021). http://dx.doi.org/10.18512/rbms2021v20e1174.
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In the western region of the State of Goias family farmers that depend onthe raising of livestock as one of their main sources of income are predominant. These farmers periodically grow maize for the production of silage in order to compensate their incomes. Considering the high cost of hybrid seeds and nitrogen fertilization, technologies that contribute to the reduction of silage production costs are required. In this sense, the objective of this study was to evaluate the response of maize genetic materials (Feroz Hybrid VIP3 and varieties SCS 156 and SCS 154), regarding silage production, bromatological composition and economic viability when inoculated with Azospirillum brasilense. The experiment was carried out on strips using a completely randomized design with four replications. Each cultivar responded differently to inoculation. For the Feroz hybrid and SCS 156 variety, the use of Azospirillum provided increases of 13.1% and 42.1% in green stem mass and 11.2 and 30.3% in silage nitrogen content, influencing the nutritional composition of the bulky food produced. For the SCS 154 variety, there was no response to inoculation. As for the economic viability, the use of inoculated SCS 156 variety presents a better rate of rentability, proving to be more economically attractive and viable to farmers.
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Kumar, Sourbh, Uttam Chandel, and Satish Kumar Guleria. "Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize." Israel Journal of Plant Sciences, January 27, 2021, 1–10. http://dx.doi.org/10.1163/22238980-bja10020.
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Abstract An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.
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Rivas-Franco, Federico, John G. Hampton, Nora A. Altier, et al. "Production of Microsclerotia From Entomopathogenic Fungi and Use in Maize Seed Coating as Delivery for Biocontrol Against Fusarium graminearum." Frontiers in Sustainable Food Systems 4 (December 10, 2020). http://dx.doi.org/10.3389/fsufs.2020.606828.
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The commercial use of the entomopathogenic fungi Metarhizium spp. in biopesticides has gained more interest since the discovery that several species of this genus are able to colonize roots. In general, commercial products with Metarhizium are formulated based on conidia for insect pest control. The process of mass production, harvesting, and formulation of infective conidia can be detrimental for conidial viability. Entomopathogenic fungi such as Metarhizium spp. are able to produce high concentrations of resistant structures, known as microsclerotia, when grown in liquid media. Microsclerotia are desiccation tolerant, with excellent storage stability, and are capable of producing high quantities of infective conidia after rehydration. The aim of this study was to evaluate microsclerotia production by different isolates of Metarhizium spp. and determine the effect of microsclerotia coated onto maize seeds on plant growth in the presence of soil-borne pathogen Fusarium graminearum. On average, ~1 × 105 microsclerotia/mL were produced by selected isolates of M. anisopliae (A1080 and F672) and Metarhizium robertsii (F447). Microsclerotia were formulated as granules with diatomaceous earth and used for seed coating, after which propagules produced around 5 × 106 CFU/g of seeds. In the presence of the plant pathogen, maize plants grown from untreated seeds had the lowest growth, while plants treated with the Metarhizium microsclerotia had significantly greater growth than the control plants. Hyphae were observed growing on and in root tissues in all the Metarhizium spp. treatments but not in samples from control plants. Metarhizium hyphal penetration points' on roots were observed 1 month after sowing, indicating the fungi were colonizing roots as endophytes. The results obtained indicate that microsclerotia can be coated onto seeds, providing plant protection against soil plant pathogens and a method to establish Metarhizium in the ecto- and endo-rhizosphere of maize roots, allowing the persistence of this biocontrol agent.
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Lohn, André Felipe, Miluse Trtikova, Ignacio Chapela, Rosa Binimelis, and Angelika Hilbeck. "Transgene behavior in genetically modified teosinte hybrid plants: transcriptome expression, insecticidal protein production and bioactivity against a target insect pest." Environmental Sciences Europe 33, no. 1 (2021). http://dx.doi.org/10.1186/s12302-021-00506-x.
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Abstract Background In 2009, Spanish farmers reported a novel weed, now identified as a relative of maize’s ancestor, teosinte, in their maize fields. Introgression of the insect resistance transgene cry1Ab from genetically modified (GM) maize into populations of this weedy Spanish teosinte could endow it with additional defense mechanisms. The aims of this study were: (1) to test if hybridization between GM maize and weedy plants from Spain is possible; (2) to understand the relationship between transgene transcription activity, concentrations of the expected transgene product (Cry1Ab protein) and the bioactivity of the latter on target insect pests following transgene flow from GM maize into Spanish teosinte plants. Results We demonstrated that hybridization between GM maize and the weedy Spanish teosinte is possible, with no observable barrier to the formation of crop/weed hybrids when teosinte served as pollen donor. When GM maize plants were used as pollen donors, significant crossing incompatibility was observed: hybrid plants produced only few “normal” seeds. Nevertheless, viable F1 seeds from GM pollen crossed onto teosinte were indeed obtained. The cry1Ab transgene was stably expressed as mRNA in all crossings and backgrounds. Similarly, toxicity on neonate Ostrinia nubilalis, presumably due to Cry1Ab protein, was consistently expressed in teosinte hybrids, with mortality rates 95% or higher after only 4 days exposure, similar to rates on parental GM maize plants. Nevertheless, no strong correlations were observed between transgene transcription levels and Cry1Ab concentrations, nor between Cry1Ab concentrations and insect mortality rates across all of the different genetic backgrounds. Conclusions Our results establish fundamental parameters for environmental risk assessments in the European context: first, we show that crop/weed hybridization in fields where maize and teosinte exist sympatrically can lead to potentially catastrophic transfer of resistance traits into an already noxious weed; second, our results question the viability of using gene dosage to model and predict ecological performance in either the intended crop plant or the undesired teosinte weed. Significant questions remain that should be addressed in order to provide a scientific, sound approach to the management of this novel weed.
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AA, Sobowale. "Possible Association Levels between Fertilizer (300 kg/Ha NPK) Application and Fungal Incidence and Viability of Stored Maize Seeds." Journal of Plant Pathology & Microbiology 04, no. 02 (2012). http://dx.doi.org/10.4172/2157-7471.1000163.
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Rojas-Lima, José Ernesto, Arturo Domínguez-Pacheco, Claudia Hernández-Aguilar, Luis Manuel Hernández-Simón, and Alfredo Cruz-Orea. "Statistical methods for the analysis of thermal images obtained from corn seeds." SN Applied Sciences 3, no. 4 (2021). http://dx.doi.org/10.1007/s42452-021-04486-8.
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AbstractDuring the last decades, digital image processing algorithms have been developed to measure external characteristics of agricultural products due to the great potential that these methods offer. So, in this research, the thermal images obtained from a thermographic camera were analysed considering two genotypes of maize seeds: crystalline and floury in their natural state, previously irradiated with a laser light source of 650 nm for exposure times of 15 s and 35 s. The methods applied in the analysis were: a) histogram to obtain the distribution of gray levels of images, b) mean value that indicates the brightness of images, c) variance which means the contrast of images, d) entropy applying both Shannon and Tsallis definitions, which provide the average self-information of images, e) estimation of the probability density of temperature variations on seeds to quantitatively characterize them from thermal images. Higher mean and variance were obtained from crystalline seeds indicating higher brightness and contrast. Furthermore, thermal images of floury seeds had higher entropy of Shannon indicating that images had greater disorder with respect to images of crystalline seeds. In the case of the entropy of Tsallis, the entropic index q could be used for characterization of seeds. Thermal images obtained from seeds with a floury structure provided a higher redundancy value for a shorter exposure time to laser light. Thus, the viability of the statistical methods of digital image processing applied to thermal imaging for the characterization of seeds is shown.
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Henga, S. A., W. M. Thagana, Hunja Hurage, and M. W. Githendu. "Assessing Seed Vigor Characters of Selected Rice (Oryza sativa L.) Genotypes Using Accelerated Ageing Method." Asian Journal of Agricultural and Horticultural Research, September 16, 2019, 1–5. http://dx.doi.org/10.9734/ajahr/2019/v4i330024.
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Rice (Oryza sativa L.) is an annual grass with the third-highest world production after sugarcane and maize. However, due to losses in vigor caused by poor seed storage, global consumption has surpassed production. Preservation of germ plasma both in-situ and ex-situ is the key to the conservation of rich biodiversity. Seed vigor is a key element of seed quality and high vigor seeds give uniform plants stand and higher yields per area. Accelerated ageing tests enable testing the vigor of stored seeds by subjecting the seeds to a particular temperature and relative humidity over time and then performing standard germination tests. Viability of most seeds normally reduces with the storage period, storage temperature and relative humidity. There is limited information on the duration of storage for rice seeds. Accelerated ageing is considered an excellent option as a vigor test when compared to seedling emergence and index of emergence speed because of the shortest time of acquisition and efficient results. Accelerated ageing tests at 0, 24, 48 and 72 hours (45ºC and 98% RH) were carried out JKUAT post-harvest laboratory using eight rice varieties’ in four replications of 100 seeds each. Data were analyzed using GENSTAT statistical package. ANOVA and T-tests at 5% significant level. Results showed that there was significant variation in both coleorhiza and coleoptile formation among rice varieties (p<0.001), treatments (p<0.001) and interaction between rice varieties and treatment (p<0.001). The difference between all treatments was significant with 72 hours’ treatment having the highest number of days to coleorhiza and coleoptile formation. The present study has shown that prolonged duration of higher temperatures results in a reduction of seed viability. The earliest coleorhiza formation was observed on day 2 and the latest on day 6. Results from this study will guide farmers and seed processors on considerations regarding storage period and storage temperature to ensure high-quality seeds.
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Olasoji, J. O., and D. J. Ogunniyan. "Genetic Variability in Physiological Seed Quality of Maize Genotypes of Different Maturity Groups." International Journal of Plant & Soil Science, December 12, 2020, 1–10. http://dx.doi.org/10.9734/ijpss/2020/v32i1730381.
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A study was conducted to investigate genetic variability in physiological seed quality using viability and vigour tests which were conducted at the seed testing laboratory of Institute of Agricultural Research and Training (IAR&T), Moor Plantation, Ibadan. The investigational materials consisted of twelve genotypes of maize of different maturity-early, intermediate and late collected from the International Institute of Tropical Agriculture (IITA), Ibadan and IAR&T, Moor Plantation, Ibadan, respectively. The experimental design used was completely randomize design (CRD) and data was collected for some viability and vigour traits and the data collected were subjected to analysis of variance. Significant differences were found among all the maize genotypes for the characters under observation. Hundred seed weight ranged from 24.6-29.3 g, 18.5-26.6 g, 22.9-29.1 g, standard germination ranged from 54.0-100.0%, 16.0-92.0%, 50.0-100% and accelerate ageing germination also ranged from 6.0-96.0%, 7.0-84.0%, 0-90.0% in the early, intermediate and late- maturing genotypes, respectively. Conductivity ranged from 5.34-17.10 µS/cm/g, 13.7-91.7 µS/cm/g and7.06-33.6 µS/cm/g with mean values of 11.14, 39.23 and 14.05 µS/cm/g in the early, intermediate and late-maturing genotypes, respectively. Variability was smaller in the late-maturing genotypes for Root Number (RN) and Shoot Length (SLT) but higher for both early and late- maturing genotypes. It was concluded from this study that seeds of early-maturing genotypes had significantly higher physical and physiological quality parameters. All the early materials used for the study tend to be homogenous for all the traits measured and selection should be practiced for hundred seed weight to increase yield potential in maize varieties.