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Journal articles on the topic 'Malmgren Modified Terrestrial Orchid'

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Published: 2 June 2025
Last updated: 31 July 2025

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1

Kaur, Saranjeet. "In vitro asymbiotic propagation of the vulnerable slipper orchid Cypripedium cordigerum D. Don." EUROPEAN JOURNAL OF ENVIRONMENTAL SCIENCES 13, no. 2 (2023): 90–95. http://dx.doi.org/10.14712/23361964.2023.10.

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This paper presents an attempt to establish a protocol for the conservation of Cypripedium cordigerum D. Don a vulnerable species of orchid using in vitro symbiotic seed germination. The suitability of four orchids seed germination media [Terrestrial orchid medium (BM), modified terrestrial orchid seed germination medium (BM-1), Malmgren modified terrestrial orchid medium (MM), Knudson C medium (KC)] was tested using different photoperiods i.e. 24-h dark or 12-h light per day. The seed capsules were harvested at two different stages of development in order to determine the effect of capsule ma
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2

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, and Snehasis Jana. "Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium." American Journal of Bioscience and Bioengineering 3, no. 6 (2015): 169–77. https://doi.org/10.11648/j.bio.20150306.15.

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Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue culture applications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The control group was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treated samples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC)
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3

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Mishra, and Snehasis Jana. "Physicochemical Evaluation of Biofield Treated Peptone And Malmgren Modified Terrestrial Orchid Medium." American Journal of Bioscience and Bioengineering 3, no. 6 (2015): 169–77. https://doi.org/10.5281/zenodo.192886.

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Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue culture applications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The control group was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treated samples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorim
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4

Kumar Trivedi, Mahendra. "Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium." American Journal of Bioscience and Bioengineering 3, no. 6 (2015): 169. http://dx.doi.org/10.11648/j.bio.20150306.15.

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5

Thakur, Uma, and Nitin Dongarwar. "ASYMBIOTIC SEED GERMINATION OF AN ANTICANCEROUS TERRESTRIAL ORCHID Eulophia nuda Lindl. AND ITS ADULTERANT Geodorum densiflorum (Lam) Schltr." Applied Biological Research 26, no. 2 (2024): 263–69. http://dx.doi.org/10.48165/abr.2024.26.01.30.

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Eulophia nuda Lindl. corms are used to cure cancer and is overexploited by the traditional medicine practitioners and medicinal plant traders. The corms of Geodorum densiflorum look morphologically similar to the corms of Eulophia nuda, hence it is used as an adulterant to the corms of Eulophia nuda, consequetly, the population of G. densiflorum is also facing the exploitation pressure. In present study, asymbiotic seed germination of both the terrestrial orchids was achieved using six media like Vacin and Went (WV) medium, Malmgren modified (MM) terrestrial orchid medium, modified Kundson C m
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6

Deconninck, Gwenaëlle, and Argyrios Gerakis. "Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.)." EuroBiotech Journal 5, no. 1 (2021): 15–23. http://dx.doi.org/10.2478/ebtj-2021-0004.

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Abstract A critical step during in vitro sexual propagation of terrestrial orchids is the treatment of the microscopic seeds with a disinfecting solution that kills bacteria and fungi attached to the seeds. This treatment is necessary to prevent infection of the culture vessels. At the same time, the treatment serves to scarify the seeds, a process that disrupts seed dormancy and initiates germination. The literature is inconclusive with respect to the proper combination of disinfecting solution strength and treatment duration. Both factors should be adapted to each species to guarantee minima
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7

Dowling, Nicole, and Manfred Jusaitis. "Asymbiotic in vitro germination and seed quality assessment of Australian terrestrial orchids." Australian Journal of Botany 60, no. 7 (2012): 592. http://dx.doi.org/10.1071/bt12133.

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Determining the seed quality and germination requirements for threatened orchid species in storage is vital for future conservation efforts. Seeds of many Australian terrestrial orchid species are held in conservation collections around the country, but few have been germinated in vitro, fuelling concerns over their long-term viability. This study tested three methods of assessing orchid seed quality; asymbiotic germination was compared with vital staining using triphenyltetrazolium chloride or fluorescein diacetate. Six culture media were examined for efficacy in promoting asymbiotic seed ger
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8

Bran Barrientos, Boris Andrés, and Jong-Yi Fang. "Influence of Photoperiod and Culture Medium on the Speed of Asymbiotic Seed Germination and Seedling Development in Spathoglottis plicata." HortScience 54, no. 9 (2019): 1570–75. http://dx.doi.org/10.21273/hortsci14214-19.

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Spathoglottis plicata is an attractive, easy-to-grow, and floriferous terrestrial orchid that has become the most important horticulturally cultivated Spathoglottis species in Southeast Asia. The present research was conducted to study the asymbiotic seed germination and seedling development of this orchid under the influence of various photoperiod and medium treatments. Seeds from 28-day-old capsule were sown on five culture media, including half-strength Murashige and Skoog medium (1/2 MS), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), Vacin and Went Modified Orchi
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9

Collins, MT, and KW Dixon. "Micropropagation of an Australian terrestrial orchid Diuris longifolia R. Br." Australian Journal of Experimental Agriculture 32, no. 1 (1992): 131. http://dx.doi.org/10.1071/ea9920131.

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A method is described for the in vitro propagation of the Australian terrestrial orchid Diuris longifolia R. Br. Explants from inflorescences of D. longifolia were successfully cultured on modified Burgeffs N3f containing the cytokinin N6 benzyladenine. Protocorm-like bodies formed after 49 days on explants taken from the basal section of flower buds and axillary nodes from inflorescences. Root formation occurred 70 days after transfer of 10-20-rnm-long shoots onto medium containing coconut water and no cytokinin, and rooted plantlets were transferred to soil and acclimatised 2-3 weeks later.
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10

Szendrák, Erika, and Paul E. Read. "Asymbiotic in Vitro Propagation of Temperate Terrestrial Orchids (Orchidaceae)." HortScience 31, no. 4 (1996): 588a—588. http://dx.doi.org/10.21273/hortsci.31.4.588a.

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The temperate native terrestrial orchids are endangered species. Their propagation from seeds poses specific problems. It is well known that orchid seeds are devoid of endosperm and in nature they need microscopic fungi in a symbiotic relationship for germination. We developed a successful asymbiotic in vitro culture method for germinating seeds of several temperate orchid species and for maintaining the cultures of young plantlets. The medium used for both germination and seedling culture was a modified FAST medium. Seeds were surface-disinfested for 10 minutes in a 10% calcium hypochlorite s
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11

Batty, A. L., M. C. Brundrett, K. W. Dixon, and K. Sivasithamparam. "New methods to improve symbiotic propagation of temperate terrestrial orchid seedlings from axenic culture to soil." Australian Journal of Botany 54, no. 4 (2006): 367. http://dx.doi.org/10.1071/bt04023.

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This research aimed to improve the success of soil transfer of terrestrial orchid seedlings after symbiotic germination in the laboratory. Three native Western Australian terrestrial orchids (Caladenia arenicola Hopper & A.P.Brown, Diuris magnifica D.L.Jones and Thelymitra crinita Lindley) were used in this study. The key to improved seedling survival on transfer to soil was found to be the use of an intermediate stage between the Petri dish and soil where larger seedlings were grown in an axenic environment with controlled humidity. There was no apparent benefit of pre-inoculating potting
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12

Zettler, Lawrence W., Sarah B. Poulter, Kris I. McDonald, and Scott L. Stewart. "Conservation-driven Propagation of an Epiphytic Orchid (Epidendrum nocturnum) with a Mycorrhizal Fungus." HortScience 42, no. 1 (2007): 135–39. http://dx.doi.org/10.21273/hortsci.42.1.135.

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Seeds of an endangered epiphytic orchid from Florida (Epidendrum nocturnum Jacquin) germinated in vitro with a mycorrhizal fungus [Epulorhiza repens (Bernard) Moore] using a technique normally applied to terrestrial orchids (symbiotic seed germination). Seeds from two sources (Fakahatchee Strand, Fla. Panther NWR) were sown on either modified oats medium (MOM) or standard oat medium (SOM) and inoculated with the fungus. Significant differences in germination were detected between the two seed sources. MOM had a significant effect on mean leaf length during incubation in vitro (F (1278) = 23.81
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13

Burkhan, Hazirah, Kirutika Selva Rajan, Suganthi Appalasamy, et al. "Effect of Cryopreservation Method Supported with Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia discolor." Plants 11, no. 7 (2022): 879. http://dx.doi.org/10.3390/plants11070879.

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This study investigated conserving an endangered terrestrial jewel orchid Ludisia discolor, using in vitro grown axillary buds. Excised segments of axillary buds (4–5 mm in length) were precultured on a modified Murashige and Skoog (MS) medium supplemented with 0.2 M sucrose for 24 h and osmoprotected in a loading solution for 20 min. Then, axillary buds were dehydrated in plant vitrification solution 2 (PVS2) for 10 min at 0 °C and incubated in liquid nitrogen for 1 h. Subsequently, axillary buds were rewarmed rapidly by dilution solution and transferred to a growth recovery medium supplement
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14

Ayesha, Mst Naznin, and S. M. Shahinul Islam. "In vitro Micropropagation and Mass Multiplication of Arundina graminifolia (D. Don) Hochr.- A Terrestrial Medicinal Orchid of Bangladesh." Plant Tissue Culture and Biotechnology 35, no. 1 (2025): 67–80. https://doi.org/10.3329/ptcb.v35i1.82245.

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The present study was undertaken to develop an effective protocol for rapid in vitro seed germination and mass propagation of Arundina graminifolia. Four basal media, viz., MS, B5, PM and modified VW, were used for germination of seeds and protocorm development. Here, MS medium showed better performance (89.31 ± 0.98%) in seed germination than that of the others. In vitro grown plantlets with 2-3 leaves were considered as explants for multiple shoot buds (MSBs) induction and its subsequent development and for that various concentration of PGRs (Kn, BAP and NAA) either single or in combination
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15

Szendrák, Erika, Paul E. Read, Eszter R. Eszéki, Elizabeth Jámbor-Benczúi, and Aniko Csillag. "Scanning Electron Microscope Studies of the Development from Germination to Mature Plant of Some Hardy Terrestrial Orchids." HortScience 30, no. 4 (1995): 870F—871. http://dx.doi.org/10.21273/hortsci.30.4.870f.

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Cultures of several orchid species [Barlia robertiana (Loisel.), Dactylorhiza fúchsii Soó, D. incarnata (L.) Soó, D. maculata (L.) Soó, D. majalis (Rchb.), D. saccifera (Brong) Soó, D. sambucina (L.) Soó, Gymnadenia conopsea (L.) R.Br., Himantoglossurn hircinum (L.) Spreng., Ophris sphegodes Mill., Orchis coriophora ssp. fragrans L., Orchis laxiflora ssp. palustris Lam., Orchis mascula L., Orchis morio L., Platanthera bifolia (L.) Rich., Spiranthes aestivalis (Poir.) Rich.] were initiated with fresh ripe seeds from desiccated fruit and 4-month-old in vitro seedlings. The medium used for
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16

Szendrák, Erika, Paul E. Read, Eszter R. Eszéki, Elizabeth Jámbor-Benczúi, and Aniko Csillag. "Scanning Electron Microscope Studies of the Development from Germination to Mature Plant of Some Hardy Terrestrial Orchids." HortScience 30, no. 4 (1995): 870F—871. http://dx.doi.org/10.21273/hortsci.30.4.870.

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Cultures of several orchid species [Barlia robertiana (Loisel.), Dactylorhiza fúchsii Soó, D. incarnata (L.) Soó, D. maculata (L.) Soó, D. majalis (Rchb.), D. saccifera (Brong) Soó, D. sambucina (L.) Soó, Gymnadenia conopsea (L.) R.Br., Himantoglossurn hircinum (L.) Spreng., Ophris sphegodes Mill., Orchis coriophora ssp. fragrans L., Orchis laxiflora ssp. palustris Lam., Orchis mascula L., Orchis morio L., Platanthera bifolia (L.) Rich., Spiranthes aestivalis (Poir.) Rich.] were initiated with fresh ripe seeds from desiccated fruit and 4-month-old in vitro seedlings. The medium used for
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17

Mondal, Tustu, Sumana Aditya, and Nirmalya Banerjee. "In vitro Axillary Shoot Regeneration and Direct Protocorm-like Body Induction from Axenic Shoot Tips of Doritis pulcherrima Lindl." Plant Tissue Culture and Biotechnology 23, no. 2 (2014): 251–61. http://dx.doi.org/10.3329/ptcb.v23i2.17526.

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An efficient protocol for in vitro propagation of an important ornamental terrestrial orchid, Doritis pulcherima Lindl. through axillary shoot and direct protocorm-like body (PLB) formation from shoot tip explants derived from six- month-old axenic seedlings has been described. Shoot tips were cultured on modified nutrient medium of Knudson’s C supplemented with 0.1% peptone and combination of various concentrations of NAA and BAP. The effect of NAA and BAP on axillary shoot formation, protocorm-like body induction and root regeneration from the explants was significant. The highest frequency
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18

Scopece, Giovanni, Nicola Criscuolo, and Salvatore Cozzolino. "In nomen omen: the effect of flower distance on female reproductive success of the lax-flowered orchid Anacamptis laxiflora (Orchidaceae)." Journal of Plant Ecology 14, no. 3 (2021): 451–64. http://dx.doi.org/10.1093/jpe/rtab002.

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Abstract Aims In multiflowered species, the architecture of inflorescences is of primary importance in shaping plant attractiveness. The aim of this study was to disentangle the role of inflorescence traits in plant female reproductive success and pollination patterns along the inflorescence in the lax-flowered orchid Anacamptis laxiflora, a terrestrial species exploiting a deceptive pollination strategy. We also evaluated whether the relationship between inflorescence traits and female reproductive success was modified by the height of surrounding vegetation and/or by population density. Meth
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19

Nabieva, Aleksandra Yurievna. "Asymbiotic seed germination and in vitro seedling development of Orchis militaris, an endangered orchid in Siberia." Journal of Genetic Engineering and Biotechnology 19, no. 1 (2021). http://dx.doi.org/10.1186/s43141-021-00223-1.

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Abstract Background Terrestrial orchids belonging to the Orchis genus are difficult to propagate and are under great pressure in their natural habitats. Studies regarding the influence of photoperiod and temperature regimes on Orchis militaris germination and morphological changes during immature seed development in vitro are scarce. Our aim was to identify photoperiod, temperature, and different nutrient media requirements for optimization of O. militaris seed germination and vigorous seedling production. Results Post-germination morphological changes were recorded with O. militaris seeds col
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20

Kaur, Saranjeet, and Kamlesh Kumar Bhutani. "Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)." Acta Societatis Botanicorum Poloniae 85, no. 2 (2016). http://dx.doi.org/10.5586/asbp.3494.

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The present study was intended to facilitate ex situ conservation of <em>Paphiopedilum venustum</em>, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving
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21

Mahendra, Trivedi, Branton Alice, Trivedi Dahryn, and Nayak Gopal. "Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium." Science Publishing Group, December 21, 2015. https://doi.org/10.5281/zenodo.813739.

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Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue culture applications. This research study was conducted to explore the influence of Mr. Trivedi's biofield energy treatment on physicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The control group was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treated samples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC), ther
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22

Johnson, S. D., and M. G. Balducci. "Settling moth pollination of a rare orchid in the Habenaria clade revealed with close‐focusing camera traps." Plant Biology, July 24, 2024. http://dx.doi.org/10.1111/plb.13696.

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Abstract It is challenging to make nocturnal observations of the natural behaviour of flower‐visiting moths as they are highly sensitive to visible artificial light sources. One solution, implemented here, is to use specially‐adapted close‐focusing camera traps that rely on infrared wavelengths for detection and illumination of small insect subjects. We investigated the pollination system of Bonatea saundersiodes, a rare African terrestrial orchid that is phylogenetically nested in the large Habenaria clade. We used modified camera traps and direct observations to study the pollination system
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23

Hampejsová, Romana, Miroslav Berka, Veronika Berková, et al. "Interaction With Fungi Promotes the Accumulation of Specific Defense Molecules in Orchid Tubers and May Increase the Value of Tubers for Biotechnological and Medicinal Applications: The Case Study of Interaction Between Dactylorhiza sp. and Tulasnella calospora." Frontiers in Plant Science 13 (June 30, 2022). http://dx.doi.org/10.3389/fpls.2022.757852.

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Terrestrial orchids can form tubers, organs modified to store energy reserves. Tubers are an attractive source of nutrients, and salep, a flour made from dried orchid tubers, is the source of traditional beverages. Tubers also contain valuable secondary metabolites and are used in traditional medicine. The extensive harvest of wild orchids is endangering their populations in nature; however, orchids can be cultivated and tubers mass-produced. This work illustrates the importance of plant-fungus interaction in shaping the content of orchid tubers in vitro. Orchid plants of Dactylorhiza sp. grow
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