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1

Gangneux, Jean-Pierre, Florence Robert-Gangneux, Guirec Gicquel, et al. "Bacterial and Fungal Counts in Hospital Air: Comparative Yields for 4 Sieve Impactor Air Samplers With 2 Culture Media." Infection Control & Hospital Epidemiology 27, no. 12 (2006): 1405–8. http://dx.doi.org/10.1086/508840.

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We compared the yields of 4 recently developed sieve impactor air samplers that meet international standard ISO 14698-1, using 2 growth media (tryptic soy agar and malt extract agar) in real conditions of use. Several hospital sites expected to have different densities of airborne microflora were selected in 2 hospitals. The Samplair MK2, Air Ideal, and Mas-100 samplers yielded higher bacterial counts than did the SAS Super-100 device (P<.05). No significant differences in fungal counts were noted between the 4 devices. The use of malt extract agar in addition to tryptic soy agar signif
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2

Heperkan, Dilek, and Ece Gökmen. "Application of Fourier Transform Infrared (FTIR) Spectroscopy for Rapid Detection of Fumonisin B2 in Raisins." Journal of AOAC INTERNATIONAL 99, no. 4 (2016): 899–905. http://dx.doi.org/10.5740/jaoacint.16-0156.

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Abstract The aim of this study was to investigate the potential use of FTIR spectroscopy as a rapid screening method to detect fumonisin produced by Aspergillus niger. A. niger spore suspensions isolated from raisins were inoculated in Petri dishes prepared with sultana raisin or black raisin extracts containing agar and malt extract agar (MEA). After 9 days of incubation at 25°C, fumonisin B2 (FB2) production on each agar plate was determined by subjecting the agar plugs to IR spectroscopy. The presence of amino group (at 1636–1639 cm−1) was especially indicative of fumonisin production in ME
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3

NÚÑEZ, FÉLIX, CARMEN D. WESTPHAL, ELENA BERMÚDEZ, and MIGUEL A. ASENSIO. "Production of Secondary Metabolites by Some Terverticillate Penicillia on Carbohydrate-Rich and Meat Substrates." Journal of Food Protection 70, no. 12 (2007): 2829–36. http://dx.doi.org/10.4315/0362-028x-70.12.2829.

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Most terverticillate penicillia isolated from dry-cured meat products are toxigenic, but their ability to produce hazardous metabolites on meat-based substrates is not well known. The production of extrolites by selected terverticillate penicillia isolated from dry-cured ham has been studied on carbohydrate-rich media (malt extract agar, Czapek yeast autolysate agar, rice extract agar, and rice), meat extract triolein salt agar, and ham slices. Chloroform extracts from the selected strains grown on malt extract agar were toxic for the brine shrimp (Artemia salina) larvae and VERO cells at a co
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4

JERMINI, MARCO F. G., KARL WEBER, and WILHELM SCHMIDT-LORENZ. "Influence of Prolonged Culture Storage on the Osmotolerance of Zygosaccharomyces Yeasts." Journal of Food Protection 50, no. 6 (1987): 514–20. http://dx.doi.org/10.4315/0362-028x-50.6.514.

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To record the effects of prolonged culture storage on the sugar tolerance of Zygosaccharomyces rouxii and Z. bailii, the fermentation behavior of three freshly isolated strains in four different glucose broths (aw values; 0.963, 0.936, 0.909 and 0.858, respectively) was compared with that of the same strains after 2 years of storage (a) on high-sugar agar slants with repeated subculturing and (b) in Biomalt (liquid malt extract) without subculturing. The trials with stock strains resulted in large reductions of both ethanol yield and production rate. Cells stored in liquid malt extract showed
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5

Sibero, Mada Triandala, Aninditia Sabdaningsih, Ocky Karna Radjasa, Agus Sabdono, Agus Trianto та Subagiyo Subagiyo. "Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli". Jurnal Kelautan Tropis 22, № 1 (2019): 9. http://dx.doi.org/10.14710/jkt.v0i0.3528.

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The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted
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6

Sibero, Mada Triandala, Aninditia Sabdaningsih, Ocky Karna Radjasa, Agus Sabdono, Agus Trianto та Subagiyo Subagiyo. "Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli". Jurnal Kelautan Tropis 22, № 1 (2019): 9. http://dx.doi.org/10.14710/jkt.v22i1.3528.

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The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted
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7

Ulrich, Sebastian, and Cornelius Schäfer. "Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media." Journal of Fungi 6, no. 3 (2020): 159. http://dx.doi.org/10.3390/jof6030159.

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Stachybotrys (S.) chartarum had been linked to severe health problems in humans and animals, which occur after exposure to the toxic secondary metabolites of this mold. S. chartarum had been isolated from different environmental sources, ranging from culinary herbs and improperly stored fodder to damp building materials. To access the pathogenic potential of isolates, it is essential to analyze them under defined conditions that allow for the production of their toxic metabolites. All Stachybotrys species are assumed to produce the immunosuppressive phenylspirodrimanes, but the highly cytotoxi
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8

Kaurav, Ajay Singh. "Screening of Effective Media for the Growth of Pyricularia grisea under In-vitro Condition." Indian Journal of Pure & Applied Biosciences 9, no. 3 (2021): 131–35. http://dx.doi.org/10.18782/2582-2845.8658.

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Pearl millet [Pennisetum glaucoma (L.) R. Br.], belongs to family Poaceae (section Paniceae), is the world’s hardiest warm-season cereal crop with the annual rainfall of 150 mm to 1000 mm.Maximum mycelial growth was recorded in potato dextrose carrot agar medium (86.33 mm) followed by Pearl millet Potato dextrose agar (83.67 mm), potato dextrose agar (78.33 mm), Pearl millet carrot dextrose agar (71.67 mm), Pearl millet potato agar (68.33 mm), Carrot dextrose agar (60.67 mm), Pearl millet grain potato dextrose agar (58.33 mm), Oat meal agar (51.67 mm), Pearl millet dextrose agar (51.67 mm), Ca
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9

Stosic, Stefan, Danijela Ristic, and Svetlana Zivkovic. "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum." Zbornik Matice srpske za prirodne nauke, no. 140 (2021): 29–44. http://dx.doi.org/10.2298/zmspn2140029s.

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Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 ?C). Isolates were sequenced for
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10

Er, Chin Ming, N. M. Sunar, A. M. Leman, and N. Othman. "Direct growth inhibition assay of total airborne fungi with application of biocide-treated malt extract agar." MethodsX 2 (2015): 340–44. http://dx.doi.org/10.1016/j.mex.2015.07.002.

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11

Skaar, I., and H. Stenwig. "Malt-yeast extract-sucrose agar, a suitable medium for enumeration and isolation of fungi from silage." Applied and environmental microbiology 62, no. 10 (1996): 3614–19. http://dx.doi.org/10.1128/aem.62.10.3614-3619.1996.

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12

Fallo, Gergonius. "Pertumbuhan Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri pada Berbagai Media." Savana Cendana 2, no. 03 (2017): 39–41. http://dx.doi.org/10.32938/sc.v2i03.41.

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Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri merupakan cendawan perusak bahan pangan yang ditemukan pada saat bahan pangan belum dipanen atau setelah bahan pangan dipanen. Pertumbuhan ketiga cendawan ini sangat dipengaruhi oleh nutrisi yang terkandung pada media tumbuh. Penelitian ini bertujuan untuk membandingkan pertumbuhan Fusarium verticillioides, Aspergillus flavus, dan Eurotium chevalieri pada beberapa media untuk isolasi dan identifikasi. Ketiga cendawan tersebut ditumbuhkan pada media yang berbeda yaitu Chloramphenicol Peptone Agar (DCPA), Dichloran 18% Glycero
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13

Kačániová, Miroslava, Simona Kunová, Jozef Sabo, et al. "Identification of Yeasts with Mass Spectrometry during Wine Production." Fermentation 6, no. 1 (2020): 5. http://dx.doi.org/10.3390/fermentation6010005.

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The aim of the present study was to identify yeasts in grape, new wine “federweisser” and unfiltered wine samples. A total amount of 30 grapes, 30 new wine samples and 30 wine samples (15 white and 15 red) were collected from August until September, 2018, from a local Slovak winemaker, including Green Veltliner (3), Mūller Thurgau (3), Palava (3), Rhein Riesling (3), Sauvignon Blanc (3), Alibernet (3), André (3), Blue Frankish (3), Cabernet Sauvignon (3), and Dornfelder (3) grapes; federweisser and unfiltered wine samples were also used in our study. Wort agar (WA), yeast extract peptone dextr
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14

Baird, R. E., T. B. Brenneman, D. K. Bell, et al. "Influence of crop rotation and flutolanil on the diversity of fungi on peanut shells." Phytoprotection 76, no. 3 (2005): 101–13. http://dx.doi.org/10.7202/706089ar.

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Soilborne pathogens of peanut (Arachis hypogaea) often survive or over winter on peanut shells left on or in the soil. The effects of different crop rotations on the peanut shell mycobiota were compared in three field trials in 1992 and repeated in 1993. In two of the trials, plots grown continuously to peanut were either treated with the fungicide flutolanil or left untreated. Rotation practices varied with location and the crops in rotation with peanut were cotton (Gossypium hirsutum), rye (Secale cereale), bahiagrass (Paspalum notatum), and corn (Zea mays). In total, 31 different gene of fu
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15

Talukdar, Rajreepa, and Kumananda Tayung. "Endophytic fungal assemblages of Zanthoxylum oxyphyllum Edgew. and their antimicrobial potential." Plant Science Today 8, no. 1 (2021): 132–39. http://dx.doi.org/10.14719/pst.2021.8.1.979.

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Zanthoxylum oxyphyllum Edgew. is a medicinal plant widely been used by the local tribal communities of Assam as an alternative source of medicine for the treatment of various diseases. In the present study, endophytic fungi associated with Z. oxyphyllum were undertaken with an aim to investigate the isolates for their antimicrobial potential. The endophytic fungi were recovered using four different media, namely, Malt Extract Agar (MEA) media, Potato Dextrose Agar (PDA) media, Water Agar (WA) media and media amended with the Plant Extract (PEA) from samples collected from three sites. Altogeth
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16

Thilagam, R., G. Kalaivani, and N. Hemalatha. "ISOLATION AND IDENTIFICATION OF PHYTOPATHOGENIC FUNGI FROM INFECTED PLANT PARTS." International Journal of Current Pharmaceutical Research 10, no. 1 (2018): 26. http://dx.doi.org/10.22159/ijcpr.2018v10i1.24404.

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Objective: The present study deals with the isolation and identification of phytopathogenic fungi. The fungal isolates were Alternaria spp (Tomato early blight), Fusarium oxysporum (Fusarium wilt), Fusarium solani (daming off and root rot), Aspergillus flavus (Ear rot) and Collectotricumspp (Anthracnose).Methods: They were isolated from infected plant parts and were identified on the basis of colony morphology andlacto phenol cotton blue (LPCB) stains were used to identifymicroscopic examination of spore structures. Pure cultures of the isolates were sub cultured and transferred onto different
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17

Gantz, Stephanie, Susanne Steudler, Hubertus Delenk, André Wagenführ, and Thomas Bley. "Zone line formation on artificial media and in hardwoods by basidiomycetes for production of spalted wood." Holzforschung 71, no. 10 (2017): 833–41. http://dx.doi.org/10.1515/hf-2016-0222.

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AbstractOne of the visual modification of wood is the formation of dark zone lines (ZLs) via interaction of fungi. The result is called spalted wood, which has hitherto been produced mainly in small batches. The main goal of the present study is to further develop techniques for rapid formation of ZLs in hardwoods. Various white rot and brown rot fungi were tested to this purpose. Initially, interactions of 148 combinations of 17 basidiomycetes in malt extract agar were evaluated and their antagonistic interactions were characterised in order to identify fungal pairs capable of rapidly forming
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18

MIHAESCU, CRISTINA, MONICA STURZEANU, and ALIN DIN. "In vitro evaluation of fungicide and bio-fungicides against isolates of Alternaria solani Sorauer." Romanian Biotechnological Letters 26, no. 1 (2021): 2295–301. http://dx.doi.org/10.25083/rbl/26.1/2295.2301.

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In vitro control of Alternaria solani included studies of different fungicides, essential oils basil and ethyl extract of Allium cepa. In Romania in the case of tomato culture it is very low due to several production constrains including diseases. One such disease is the early blight caused by fungus Alternaria solani (Ell.) Martin. The isolate used in this study was obtained from tomato and it was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and Czapek Dox medium. Using chlorothalonil, thiophanate methyl, prochloraz, tebuconazole and cooper sulphate, they w
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19

MARTÍN, ALBERTO, EMILIO ARANDA, MARÍA J. BENITO, FRANCISCO PÉREZ-NEVADO, and MARÍA G. CÓRDOBA. "Identification of Fungal Contamination and Determination of Mycotoxigenic Molds by Micellar Electrokinetic Capillary Chromatography in Smoked Paprika." Journal of Food Protection 68, no. 4 (2005): 815–22. http://dx.doi.org/10.4315/0362-028x-68.4.815.

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The purpose of this work was to analyze the fungal contamination in smoked and unsmoked paprika processed from different cultivars of pepper and to investigate the ability of these and other mycotoxigenic molds to grow and synthesize mycotoxins in smoked paprika. Eighteen mycotoxins were evaluated using micellar electrokinetic capillary chromatography. No relevant differences were found in fungal contamination between smoked and unsmoked paprika. The number of yeasts obtained was low, ranging from 0.4 to 3.29 log CFU g−1; most of the yeast strains were identified as Cryptococcus spp. followed
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Amponsah, N. T., M. Walter, and R. W. A. Scheper. "Agar media for isolation of Neonectria ditissima from symptomatic and asymptomatic apple tissues and production of infective conidia." New Zealand Plant Protection 67 (January 8, 2014): 116–22. http://dx.doi.org/10.30843/nzpp.2014.67.5741.

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European canker pathogen caused by the fungus Neonectria ditissima could not be isolated when infected woody tissues were plated directly onto potato dextrose agar (PDA) malt extract agar (MEA) or acidified PDA (pH 45) However the alternative media water agar (WA) and apple sapamended water agar (ASAWA) were successfully used to isolate the pathogen In the preparation of ASAWA green shoots of 1yearold fresh Scilate apple were frozen overnight then allowed to thaw The tissues were then centrifuged and the sap collected to prepare the medium Neonectria ditissima could be isolated from both sympt
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Mihaescu, Cristina, Madalina Militaru, and Madalina Butac. "The Influence of Some Sources of Nitrogen on the Growth and Development of the Phomopsis Incarcerata Pathogen (SACC.) Hohnel." Revista de Chimie 70, no. 11 (2019): 4000–4002. http://dx.doi.org/10.37358/rc.19.11.7691.

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Phomopsis incarcerata, known as the pathogen which caused the dieback of Rosa branches was detected in many orchards in Romania. Our investigations have approached a series of bio-ecological aspects of this pathogen: isolation, purification and obtaining the pathogen; determination and identification of the pathogen; establishing in vitro parameters of fungal development (nitrogen source). The isolate used in this study was obtained from Rosa spp. branches and was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and water agar which included five amino-acids: cy
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Sugijanto, Noor Erma, H. Putra, F. Pritayuni, N. Albathaty, and Noor Cholies Zaini. "DAYA ANTIMIKROBA EKSTRAK Lecythophora sp., ENDOFIT YANG DIISOLASI DARI Alyxia reinwardtii." Berkala Penelitian Hayati 15, no. 1 (2009): 37–44. http://dx.doi.org/10.23869/bphjbr.15.1.20098.

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The antimicrobial potential of two endophytic fungi isolated from Alyxia reinwardtii BL, towards selected bacteria (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis FNCC 0059, Pseudomonas aeruginosa ATCC 27853, Salmonella typhi) and fungi (Candida albicans) was tested using ethyl acetate, n-hexane and n-buthanol extracts of fungi cultivated under malt extract liquid fermentation. The extracts were evaluated for its antimicrobial activity by disc diffusion method followed its MIC by agar dilution. Bioautography assay for activity-directed fractionation were also
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T N, Anusha, Nischitha R, and Shivanna M B. "Diversity of Mycoflora in Root and Rhizosphere Regions of Alloteropsis Cimicina (L.) Stapf." Shanlax International Journal of Arts, Science and Humanities 8, S1-Feb (2021): 31–35. http://dx.doi.org/10.34293/sijash.v8is1-feb.3928.

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Endophytic fungi described as the internal mycota of living plants. The present study is an attempt to document mycoflora diversity of root, rhizosphere, bulk soil of grass Alloteropsis cimicina (L.) Stapf a less known species from North East India in Bhadra reserve forest region in two different seasons. Isolation, fungal community from collected samples by Moist blotter method, Potato dextrose agar (PDA) method, Malt extract agar (MEA) methodStatistical analysis were done and the mean data by Simpson Diversity Index Shannon Evenness Shannon Diversity Index(H1 ) Simpson Evenness, Colonization
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Suad A Al-Hilu and Wisam H Al-Shujairi. "Molecular characterization of covalently closed circular plasmid DNA in local Streptomyces strains isolated from soil with detection of antibiotic production and antibacterial activity." International Journal of Research in Pharmaceutical Sciences 10, no. 3 (2019): 2142–52. http://dx.doi.org/10.26452/ijrps.v10i3.1441.

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Various of Streptomyces species have two kinds of plasmids, circular plasmids (8 to 31 kb) and linear plasmids (12 to 1700 kb). Covalently closed circular (CCC) plasmids are profuse in the genus of Streptomyces and involved in production and resistance of antibiotics by genetic controlling. We collected fifty clinical soil samples from different regions in Al-Najaf Al-Ashraf province/Iraq. The samples included five from Al-Ghadeer Quarter, five from Al-Karama Quarter, 10 from Kufa University, five from Al-Ameer Quarter, four from Al-Forat Quarter, 10 from North Quarters and eleven from desert
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Scheper, R. W. A., B. M. Fisher, N. T. Amponsah, and M. Walter. "Effect of culture medium light and air circulation on sporulation of Neonectria ditissima." New Zealand Plant Protection 67 (January 8, 2014): 123–32. http://dx.doi.org/10.30843/nzpp.2014.67.5742.

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In culture most isolates of Neonectria ditissima the causal organism of European canker do not produce conidia Those that do often produce singlecelled conidia rather than the typical multicelled conidia that are found in nature A series of experiments was conducted to determine the conditions needed for conidium production Four culture media (malt extract agar modified Matsushimas medium (MM) apple sap amended water agar (ASAWA) and water agar) five light regimes including darkness near ultra violet (NUV) light and white fluorescent light and three plate sealing patterns were tested Subcultur
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Yuyama, Kamila Tomoko, Jadergudson Pereira, Cristina Sayuri Maki, and Noemia Kazue Ishikawa. "Daldinia eschscholtzii (Ascomycota, Xylariaceae) isolated from the Brazilian Amazon: taxonomic features and mycelial growth conditions." Acta Amazonica 43, no. 1 (2013): 1–8. http://dx.doi.org/10.1590/s0044-59672013000100001.

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The Amazon has a high diversity of fungi, including species of the genus Daldinia (Ascomycota, Xylariaceae), which produce secondary metabolites with recognized nematicidal and antimicrobial activity. The ecological role of Daldinia is important, as stromata serve as refuges to many insects and arthropodes, and the fungi contribute to the degradation of vegetable organic matter. The aim of this study was to analyze the taxonomic features and mycelial growth conditions in vitro of a Daldinia specimen collected in the Brazilian Amazon. Morphological and molecular studies of the fungus identified
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Johnson, Gary C. "A comparison of the efficacy of six media for isolating wood-decaying Hymenomycetes." Canadian Journal of Microbiology 41, no. 1 (1995): 104–7. http://dx.doi.org/10.1139/m95-014.

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Six media reported to be useful for isolating Hymenomycetes from decaying wood were compared. Plates containing these media were inoculated with small segments taken from decaying wood collected in the field. Hymenomycetes and other microorganisms that grew from the segments were recorded. A malt extract – potato dextrose agar medium that incorporated benomyl, neomycin, and streptomycin was the most successful at isolating the white or brown rot fungi.Key words: isolation media, Hymenomycetes, Basidiomycotina, wood decay fungi, benomyl.
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Fajarningsih, Nurrahmi Dewi, Muhammad Nursid, and Ekowati Chasanah. "Screening of Antitumor Bioactivity of Fungi Associated with Macro Algae and Sponge from Indrayanti Beach, Jogjakarta." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 8, no. 2 (2014): 47. http://dx.doi.org/10.15578/squalen.v8i2.25.

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This research was aimed to isolate marine derived-fungi which associated with macro algae and sponge from Indrayanti Beach, Jogjakarta and to screen the antitumor (T47D and HeLa) bioactivity of the fungi extracts. Three solid media of MEA (malt extract agar), GPY (glucose peptone yeast) and MFM (minimal fungi medium) were used as isolation medium. Each of the pure fungi isolates was then cultivated in 100 ml of liquid medium for 4 weeks at room temperature (27-28°C) in static conditions. The antitumor activity of the fungi extracts were tested against breast tumor cells (T47D) and cervical can
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Carvalho, Daniel Diego C., Peter W. Inglis, Zilá R. de Ávila, Irene Martins, Paulo Henrique P. C. Muniz, and Sueli C. M. de Mello. "Morphological Characteristics and Genetic Variability of Trichoderma spp. From Conventional Cotton Crop Soils in Federal District, Brazil." Journal of Agricultural Science 10, no. 8 (2018): 146. http://dx.doi.org/10.5539/jas.v10n8p146.

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This study aimed to characterize 41 isolates of Trichoderma from conventional cotton crops soils as the morphological and cultural characteristics and to investigate polymorphism, using RAPD markers. The most common group comprised 80.6% of the isolates and was identified as a strain of Trichoderma harzianum. The others species were T. aureoviride (7.3%), T. viride (7.3%) and T. crassum (4.8%). The four species were morphologically distinct in the evaluated characteristics such as colony appearance and mycelium growth rate after being grown on malt extract agar (MEA), potato dextrose agar (PDA
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OWCZAREK-KOŚCIELNIAK, MAGDALENA, and KATJA STERFLINGER. "First records of Knufia marmoricola from limestone outcrops in the Wyżyna Krakowsko-Częstochowska Upland, Poland." Phytotaxa 357, no. 2 (2018): 94. http://dx.doi.org/10.11646/phytotaxa.357.2.2.

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Studies on microcolonial fungi (MCF) inhabiting limestone formations in the Wyżyna Krakowsko-Częstochowska Upland (Poland) resulted in isolation of Knufia marmoricola. This is the first report of this species in Poland, which was previously known from the Mediterranean basin. Morphological analyses have shown a high variability dependent on used mycological media. Malt extract agar (MEA) proved to be the most suitable medium for morphological observations. Cultivation on potato dextrose agar (PDA) and dichloran rose-Bengal with chloramphenicol medium (DRBC) resulted in growth of mycelium lacki
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Siregar, Resmi Rumenta. "ISOLASI DAN IDENTIFIKASI KAPANG PADA PINDANG BANDENG (Chanos chanos) PRESTO." Jurnal Kelautan dan Perikanan Terapan (JKPT) 2, no. 1 (2019): 15. http://dx.doi.org/10.15578/jkpt.v2i1.7614.

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Ikan pindang adalah salah satu olahan yang sangat disukai oleh masyarakat Indonesia. Hal ini dapat dilihat dari produksi ikan pindang yang setiap tahunnya mengalami peningkatan. Sebagai contoh di Kabupaten Bogor, produksi ikan pindang pada tahun 2013 sebesar 3.643,56 ton, meningkat menjadi 10.334,44ton pada tahun 2015. Ikan pindang disisi lain, sangat mudah mengalami kemunduran mutu disebabkan masih tingginya kadar air, pengemasan yang tidak memenuhi standar serta proses pengolahan yang pada umumnya kurang menerapkan prinsip sanitasi yang baik. Penelitian ini bertujuan untuk mengisolasi dan me
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Garrett, Alexandre Techy de Almeida, Álvaro Figueredo Santos, Afonso Figueiredo Filho, Evandro Vagner Tambarussi, Arthur Birais Gonçalves, and Flávio Augusto de Oliveira Garcia. "Mycelial growth and sporulation of Apoharknessia eucalyptorum on different culture media." Summa Phytopathologica 45, no. 3 (2019): 272–78. http://dx.doi.org/10.1590/0100-5405/207327.

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ABSTRACT Apoharknessia eucalyptorum was first described in 2017 and identified on leaves of Eucalyptus dunnii in Southern Brazil. However, information about in vitro cultivation for complementary studies is lacking. In the present study, leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media: malt extract agar (MEA); potato dextrose agar (PDA); V8 juice agar (V8); and bean dextrose agar (BEAN), under constant lighting. Apoharknessia eucalyptorum caused leaf blig
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Sedjati, Sri, Ambariyanto Ambariyanto, Agus Trianto, et al. "Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria." Jurnal Kelautan Tropis 23, no. 1 (2020): 127. http://dx.doi.org/10.14710/jkt.v23i1.7049.

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Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3,
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Kocic-Tanackov, Suncica, Gordana Dimic, Jelena Levic, Ljiljana Mojovic, and Jelena Pejin. "Contamination of cakes with toxigenic molds." Zbornik Matice srpske za prirodne nauke, no. 124 (2013): 213–26. http://dx.doi.org/10.2298/zmspn1324213k.

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The total number of molds in cakes ranged up to 9.0 ? 102 CFU/g. The highest number of molds was isolated on Dichloran 18% Glycerol Agar (DG18), and the lowest on Malt Yeast Extract 50% Glucose Agar (MY50G). Mycopopulation of cakes composed of species of genera Acremonium, Alternaria, Aspergillus, Cladosporium, Eurotium, Mucor, Phialophora, Penicillium, Fusarium and Ulocladium. Dominant species were A. niger, P. aurantiogriseum, P. brevicompactum, P. hirsutum, F. proliferatum and A. alternata. The most common potential producers were those of ochratoxin A (50.50%). The potential producers of f
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Viegas, Carla, Marta Dias, Beatriz Almeida, et al. "Loading Rates of Dust and Bioburden in Dwellings in an Inland City of Southern Europe." Atmosphere 12, no. 3 (2021): 378. http://dx.doi.org/10.3390/atmos12030378.

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Sampling campaigns indoors have shown that occupants exposed to contaminated air generally exhibit diverse health outcomes. This study intends to assess the deposition rates of total settleable dust and bioburden in the indoor air of dwellings onto quartz fiber filters and electrostatic dust collectors (EDCs), respectively. EDC extracts were inoculated onto malt extract agar (MEA) and dichloran glycerol (DG18) agar-based media used for fungal contamination characterization, while tryptic soy agar (TSA) was applied for total bacteria assessment, and violet red bile agar (VRBA) for Gram-negative
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36

Pacumbaba, R. P., and R. O. Pacumbaba. "Shiitake Mushroom Growth on the Formulated Culture Media, Production of Spawn, and Basidiocarps in the Laboratory." HortTechnology 9, no. 1 (1999): 85–90. http://dx.doi.org/10.21273/horttech.9.1.85.

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Culture media YMMBSA (yeast extract, malt extract, multigrain oatmeal, brown sugar, agar), YVMBSA (yeast extract, V-8 vegetable juice, multigrain oatmeal, brown sugar, agar), and YVMSA (yeast extract, V-8 vegetable juice, multigrain oatmeal, sucrose, agar) and broths YVMBS (yeast extract, V-8 vegetable juice, multigrain oatmeal, brown sugar), YVMS (yeast extract, V-8 vegetable juice, sucrose), and MVBS (multigrain oatmeal V-8 vegetable juice brown sugar) were formulated and demonstrated to be excellent media and broths for growing shiitake mushrooms [Lentinula edodes (Berk.) Pegler] in the lab
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Chauvet, Eric, and Keller Suberkropp. "Temperature and Sporulation of Aquatic Hyphomycetes." Applied and Environmental Microbiology 64, no. 4 (1998): 1522–25. http://dx.doi.org/10.1128/aem.64.4.1522-1525.1998.

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ABSTRACT Temperature appears to be an important factor affecting the occurrence and distribution of aquatic hyphomycetes, the dominant leaf litter-decomposing fungi in streams. We compared conidium production by eight species of aquatic hyphomycetes grown on yellow poplar leaves in stream-simulating microcosms at three temperatures (15, 20, and 25°C). The greatest conidium production occurred at 15°C for one species, 20°C for two species, and 25°C for two species. Two species produced similar numbers of conidia at 20 and 25°C, and one species produced similar numbers of conidia at all three te
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Szulc, Justyna, Tomasz Ruman, and Beata Gutarowska. "Metabolome profiles of moulds on carton-gypsum board and malt extract agar medium obtained using an AuNPET SALDI-ToF-MS method." International Biodeterioration & Biodegradation 125 (November 2017): 13–23. http://dx.doi.org/10.1016/j.ibiod.2017.08.002.

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Rai, Barun, Sekhar Bandyopadhyay, Avisak Thapa, Adeetya Rai, and Deewakar Baral. "Morphological and cultural characterization of Phyllosticta zingiberi (Ramkr.) causing leaf spot disease of ginger." Journal of Applied and Natural Science 9, no. 3 (2017): 1662–65. http://dx.doi.org/10.31018/jans.v9i3.1418.

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Isolation of ginger (Zingiber officinale) leaf spot pathogen form the UBKV farm field was done in the laboratory. The isolated pathogen was identified as Phyllosticta zingiberi on the basis of morphological characters as documented in taxonomic keys. The microscopic observation revealed that the pycnidia were globose to subglobose with dark brown colour measuring 124.16 μm × 2.35 μm in average. The pycnidio spores were hyaline, oval to bullet shaped, monoguttulate measuring 4.02 μm × 2.35 μm in average. Among the different media tested for growth highest growth was recorded in Oat meal agar (2
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Baral, Bikash, Prabina Rana, and Bijaya Laxmi Maharjan. "ANTIMICROBIAL POTENTIALS OF ENDOPHYTIC FUNGI INHABITING RHODODENDRON ANTHOPOGON D. DON." Ecoprint: An International Journal of Ecology 18 (December 20, 2013): 39–44. http://dx.doi.org/10.3126/eco.v18i0.9397.

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Fungal endophytes have been studied from Rhododendron anthopogon D. Don Manaslu Conservation Area in Nepal. The endophytes were isolated from different parts of Rhododendron viz., root, stem and leaf using potato dextrose agar, malt extract agar and water agar. Altogether eighteen fungal endophytes belonging to nine genera were isolated. The endophytic species isolates belong to the genera Stemphylium, Alternaria, Penicillium, Aspergillus, Trichoderma, Papulaspora, Hansfordia, Wardomyces and Geotrichum. Out of 18 fungal isolates, 8 isolates could display antimicrobial activity inhibiting at le
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Abdullah, Berivan A., and Asia A. M. Saadullah. "Isolation and Identification of Yeasts from Indoor Swimming Pools in Duhok City With Special Concern to The Effect of Some Disinfectant Agents." Science Journal of University of Zakho 5, no. 2 (2017): 154. http://dx.doi.org/10.25271/2017.5.2.360.

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The present study was conducted to study the level of yeast contamination in indoor public swimming pools in Duhok city. A total of 230 samples (50 water sample) were taken from swimming pool water and (180 swab samples) were taken from walls, flour of bathrooms, dressing rooms and flour around the pool in five indoor public swimming pools from September 2014 to February 2015. All samples were examined for the presence of yeasts using three culture media (Potato Dextrose Agar, Malt Extract Agar and Sabouraud's Dextrose Agar). The highest number of Yeast genera was in November (6) followed by O
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Hamid, Norul Hisham, Othman Sulaiman, Azmy Mohammad, and Norasikin Ahmad Ludin. "The Decay Resistance and Hyphae Penetration of BambooGigantochloa scortechiniiDecayed by White and Brown Rot Fungi." International Journal of Forestry Research 2012 (2012): 1–5. http://dx.doi.org/10.1155/2012/572903.

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The decay resistance and hyphae penetration of bambooGigantochloa scortechiniidecayed by white and brown rot fungi were investigated using scanning electron microscope (SEM). The bamboo grown in natural stand from three different age classes of 0.5, 3.5, and 6.5 years was harvested, oven dried, sterilised, and exposed to agar media containing 4% malt extract and 2% agar technical (no. 3) under laboratory condition for 8 weeks. The deterioration was expressed as percentage of weight loss, and the decay resistance classes were measured according to ASTM D 2017-81 (1986). This study found that th
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Javeed, Faisal, Memuna Ghafoor Shahid, and Ali Javed. "Effect of Different Operational Parameters on Bio-degradation of Chicken Feathers by Aspergillus niger: Investigation Under Submerged Fermentation Process." Biological Sciences - PJSIR 63, no. 2 (2020): 71–76. http://dx.doi.org/10.52763/pjsir.biol.sci.63.2.2020.71.76.

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Fungal strain, Aspergillus niger (ATCC 1015) has ability to grow on keratinous material therefore, it was selected for the investigating bio-degradation of chicken feathers. Different operational parameters were studied under submerged fermentation process i.e. effect of substrate concentration, effect of pH, effect of incubation temperature, effect of yeast extract concentration and effect of volume of fermentation medium. A. niger was grown on solid medium of malt extract and agar, due to its ability of rapid growth on it. Complete bio-degradation of the substrate was achieved after 5 days (
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Amiri, A., A. I. Zuniga, J. Mertely, and N. A. Peres. "First Report on Resistance to Pyraclostrobin, Thiophanate-methyl, Fenhexamid and Boscalid in Botrytis cinerea from Eucalyptus Seedlings in Florida Greenhouses." Plant Disease 98, no. 6 (2014): 851. http://dx.doi.org/10.1094/pdis-10-13-1107-pdn.

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Botryotinia fuckeliana de Bary (anamorph Botrytis cinerea Pers.) is an ubiquitous plant pathogen causing gray mold disease on more than 200 crops grown in the field or in greenhouses. Eucalyptus seedlings originating from three different greenhouses showing stem lesions were submitted to the Gulf Coast Research and Education Center Disease Clinic in June 2012. Ten single spore isolates of B. cinerea were obtained and tested for sensitivity using spore germination and germ tube elongation assays described previously (4). Fungicides tested were pyraclostrobin at 100 μg/ml (Cabrio, BASF, Research
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Zugic-Petrovic, Tanja, Dragana Stanisavljevic, Predrag Ilic, et al. "Effect of water activity on the radial growth of fungi isolated from dry-cured sheep ham, in vitro (Serbia)." Zbornik Matice srpske za prirodne nauke, no. 134 (2018): 65–75. http://dx.doi.org/10.2298/zmspn1834065z.

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In the Western Balkans, traditional dry-cured sheep ham called Pastrma or Stelja is produced. Dry-cured sheep ham from Sjenica is produced in a very complex manner, and a prerequisite for its production is the sanitary safety of raw materials in accordance with veterinary and sanitary regulations. Isolation and preliminary identification of fungi from sheep ham were carried out in this study, as well as in vitro testing of the effects of water activity (aw) on the growth of isolated fungi. Fungi were isolated from 9 samples of dry-cured sheep meat taken from three households in the area of Sje
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46

Plaszkó, Tamás, Zsolt Szűcs, Zoltán Kállai, Hajnalka Csoma, Gábor Vasas, and Sándor Gonda. "Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)." Metabolites 10, no. 11 (2020): 451. http://dx.doi.org/10.3390/metabo10110451.

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The interaction between plant defensive metabolites and different plant-associated fungal species is of high interest to many disciplines. Volatile organic compounds (VOCs) are natural products that are easily evaporated under ambient conditions. They play a very important role in inter-species communication of microbes and their hosts. In this study, the VOCs produced by 43 different fungal isolates of endophytic and soil fungi during growth on horseradish root (Armoracia rusticana) extract or malt extract agar were examined, by using headspace-gas chromatography-mass spectrometry (headspace-
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47

Olatinwo, Rabiu, and Stephen Fraedrich. "An Acaromyces Species Associated with Bark Beetles from Southern Pine Has Inhibitory Properties Against Raffaelea lauricola, the Causal Pathogen of Laurel Wilt Disease of Redbay." Plant Health Progress 20, no. 4 (2019): 220–28. http://dx.doi.org/10.1094/php-06-19-0039-rs.

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Laurel wilt is a destructive disease of redbay (Persea borbonia) and other species in the laurel family (Lauraceae). It is caused by Raffaelea lauricola, a fungal symbiont of the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae), cointroduced into the United States around 2002. During assessments of fungi associated with bark beetles from loblolly pine, an unknown fungus was isolated that appeared to have broad-spectrum antifungal activities. In this study, we identified the unknown fungus and determined the inhibitory effect of its secondary metabolites on R. lauricola.
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48

Milijasevic, Tanja. "Effect of temperature on the mycelial growth of the fungus Sphaeropsis sapinea." Bulletin of the Faculty of Forestry, no. 94 (2006): 211–22. http://dx.doi.org/10.2298/gsf0694211m.

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Effect of temperature on mycelial growth was tested on 11 isolates from six pine species - P. nigra, P. sylvestris, P. halepensis, P. heldreichii, P. pinaster and P. pinea, originating from Serbia and Montenegro. The optimal temperature for mycelial growth of the fungus S. sapinea on potato-dextrose agar (PDA) and malt-extract agar (MEA) media was 28?C. However, a very similar colony growth occurred also at 25?C. The growth of all the tested isolates was better on PDA than on MEA. In addition to faster growth, on PDA the fungus showed the physiological activity in the wider temperature interva
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Naz, Sumaira, Salik N. Khan, and Muhammad N. Chaudhry. "EVALUATION OF SOILBORNE PLANT PATHOGENIC FUNGAL COUNT IN VARIOUS LAND USE TYPES OF DISTRICT OKARA." Pakistan Journal of Phytopathology 30, no. 1 (2018): 69. http://dx.doi.org/10.33866/phytopathol.030.01.0422.

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A field survey was conducted in district Okara to determine existing plant pathogenic fungal count in various land use types. The target sites were classified as orchard, barren land, fallow land, cultivated land and forest land use types. Field soil samples were collected from these land use types at different depths of 15, 30 and 45 cm respectively. Dilution of soil samples was made in sterilized distilled water and 1ml was poured on to the malt extract agar (MEA) medium in 90 mm diameter Petri plates incubated at 25±2°C for 5-7 days. Purified fungal colonies of Mucor spp., Fusarium solani,
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Iqbal, Muhammad, Sumera Naz, Salik N. Khan, et al. "Optimization of Culture Conditions for Mycelial Growth and Sporulation of Myrothecium roridum." International Journal of Phytopathology 10, no. 1 (2021): 01–07. http://dx.doi.org/10.33687/phytopath.010.01.3415.

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Culture and nutrition conditions of Myrothecium roridum Tode were optimized by conducting a series of interlined experiments on a growth medium, temperature, pH, and photoperiod. In contrast, relation of culture age with virulence was measured by fungal development on young leaves of bitter gourd. The physiological response was measured on colony radial growth and spore production. Among the six test growth media, i.e., nutrient agar (NA), potato dextrose agar (PDA), Czapek-Dox agar (CDA), glucose agar (GA), malt extract agar (MEA), and bitter gourd agar (BGA), the highest radial growth (77 mm
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