Dissertations / Theses on the topic 'Mango crops'

This thesis is a comparative study of carbon and nitrogen flux dynamics following sole and combined application of mango leaf litter mulch and inorganic fertiliser in highly weathered tropical mango orchard soils. In doing so, the thesis investigated litter decomposition dynamics and litter carbon mass balance and provided baseline datasets of seasonal and annual reactive nitrogen losses (through hydrological and gaseous pathways) under the tropical environmental conditions of Northern Territory Australia.

O objetivo principal deste estudo foi o desenvolvimento e validação de metodologia de análise de multiresíduos para quantificação de resíduos de pesticidas em manga. Foram analisados 32 pesticidas, sendo dos seguintes grupos: 9 Organofosforados, 17 Organoclorados e 6 triazois sendo que neste mesmo método foi analisado novamente o Organofosforado fention. Os organofosforados foram analisados em cromatógrafo a gás (CG) acoplado ao detector fotométrico de chamas e os organoclorados foram analisados em cromatógrafo a gás (CG) acoplado ao detector de captura de elétrons (ECD). Os triazois foram analisados em cromatógrafo a gás acoplado a espectrômetro de massa (CG/MS). A curva de linearidade para todos os padrões analíticos, foi realizada em triplicata e com 6 diferentes concentrações. As fortificações das amostras foram realizadas em dois níveis de concentração, no limite do método e 10 vezes o limite do método, sendo que a concentração do limite de quantificação do método (LOQ) foi realizada com sete repetições (N= 7) e a concentração de 10 vezes o LOQ foi realizada com N= 5, usando-se mangas tratadas no sistema orgânico (livres de pesticidas). Os métodos desenvolvidos e validados neste estudo apresentaram-se: a) Linear na faixa de: 0,1 - 4,0 µg/mL para o triclorfon e malation; 0,05 – 2,0 µg/mL para os demais organofosforados; 0,05 – 1,6 µg/mL para os organoclorados; 0,1 – 1,0 µg/mL para o paclobutrazol; 0,25 - 2,5 µg/mL para o fention e bromuconazol; 0,5 – 5,0 µg/mL para o tetraconazol e tebuconazol e 1,0 – 10 µg/mL para o procloraz e difenoconazol respectivamente, uma vez que o coeficiente de determinação foi maior que 0,99 para todos os ativos. b) Específicos, pois o nível de contaminação e/ou interferente na análise do branco dos reagentes e da amostra testemunha, foram inferiores a 30% do limite de quantificação. c) Exato, Preciso e Repetitivo, pois todos os ativos apresentaram recuperação dentro do intervalo de aceitabilidade de 70 a 120%, com coeficiente de variação porcentual (CV%) inferior a 15% em relação à média global de todos os níveis de fortificação. Foram também analisados os mesmos pesticidas em 15 amostras de mangas compradas nos supermercados de Piracicaba, procedentes do Nordeste do Brasil. Em nenhuma das amostras foram encontrados resíduos acima do limite estabelecido por lei ou acima do limite de quantificação deste método, no caso dos pesticidas não autorizados.
The principal objective of this study was the development and validation of a multiresidue analytical methodology for the quantification of pesticide residues in mangos. Quantitative analyses were made for 32 pesticides, from the following classes: organophosphorus (9), organochlorine (17) and triazole (6). The results from the method include the first analysis for the organophosphorus pesticide fenthion. The organophosphorus pesticides were analyzed by a gas chromatograph (GC) coupled to a flame photometric detector, while the organochlorine pesticides were analyzed using a gas chromatograph (GC) coupled to an electron capture detector (ECD). The triazoles were analyzed with a gas chromatograph coupled to a mass spectrometer (GC/MS). For each of the analytes, calibration curves were constructed in triplicate using solutions with six different concentrations, prepared from the analytical standards. Recovery experiments were performed at two levels of fortification, the limit of quantification of the method (LOQ) and 10 times this limit. Seven repetitions (N = 7) were performed at the limit of quantification, and five (N = 5) at the 10 times higher level of fortification. Mangos raised by organic farming methods (free from pesticides) were used for the recovery experiments. The methods developed and validated in this study have the following characteristics: a) linear range: 0.1 – 4.0 µg/mL for trichlorfon and malathion; 0.05 – 2.0 µg/mL for the other organophosphorus pesticides; 0.05 – 1.6 µg/mL for the organochloro pesticides; 0.1 – 1.0 µg/mL for paclobutrazol; 0.25 – 2.5 µg/mL for fenthion and bromuconazole; 0.5 – 5.0 µg/mL for tetraconazole and tebuconazole; and 1.0 – 10.0 µg/mL for prochloraz and difenoconazole, in each case the coefficient of determination was greater than 0.99. b) Specific: thus the level of contamination and/or intereference in analysis from the reagent blank and the control sample amounted to less than 30% of the limit of quantification. c) Exact, Precise and Repeatable, for each of the analytes the recovery was found to be within the acceptable interval of 70 to 120%, and with a coefficient of variation, expressed as a percentage, of less than 15% in relation to the global average of the results obtained at the two levels of fortification. Analyses were made for the same pesticides in 15 samples of mangos bought from supermarkets in Piracicaba, which were raised in the Northeast of Brazil. None of these samples contained residue concentrations above the limit established by law or, in the case of non-authorized pesticides, above the limit of the quantification of the current methodology.

The oriental fruit fly, Bactrocera dorsalis Hendel, is an economic tephritid that invaded Senegal in 2004 (Vayssieres et al. 2011). This work determined 1) Impact of B. dorsalis on mango crop production and value, and marketing practices, 2) Effect of neem and kaolin on its behavior and development (laboratory and field), and 3) Effect of treated soil with neem seed cake (NSC) and neem seed powder (NSP) on the larval-pupal survival and development (laboratory and field). Results revealed crop value (price/kg) was associated with infestation levels. P<0.0001, crop value decreased in highly infested orchards. Southern Orchards were heavily infested than northern orchards. Early harvest, orchard sanitation, decreasing the purchase quantity, sorting infested mangoes and lowering prices were strategies used by growers and traders. Laboratory bioassays showed females landed and spent time on fruit identically on control and neem. On kaolin there were fewer landings and less time spent. P<0.0001, mean pupae was higher in control than in treated mangoes. In the field, percent of infested mangoes was higher in neem than in control and kaolin. However, the number of emerged flies was higher in the control than in either the neem or kaolin-treated fruit. Treated soil with NSC and with NSP did not have effect on pupation; all larvae pupated in the lab. However, treated soil decreased significantly the number of emerged flies. In the field, there were no significant differences between untreated and treated soil in number of emerged flies.
Master of Science in Life Sciences

The determination of crop coefficient (Kc) with adequate methodology is important to quantify regional water requirement. Jatropha (Jatropha curcas L.) Kc is still unknown and this information will be essential to provide reliable irrigation parameters, as well as for crop zoning. The objective of this study was to determine jatropha actual crop evapotranspiration (ETc) and Kc from 1st to 4th growing year, and correlate Kc with leaf area index (LAI) and cumulative thermal unit (CTU). The experiment was performed from March 2012 to August 2015 at \"Luiz de Queiroz\" College of Agriculture (ESALQ)/University of São Paulo (USP), at Piracicaba city, SP, Brazil. The experiment was divided into center pivot, drip, and rainfed treatments. Two large weighing lysimeters (12 m2 each lysimeter) per treatment were used to determine jatropha ETc (one plant per lysimeter). Reference evapotranspiration (ET0) was determined by Penman-Monteith method from a weather station data situated close to the treatments. Daily Kc was determined for the two irrigated treatments by the ration between ETc and ET0 (Kc=ETc/ET0). LAI was determined using the LAI-2200 plant canopy analyzer, which was previously calibrated for jatropha canopy type. In all growing years, LAI was almost zero at the beginning of vegetative stage, increasing until a maximum during productive stage, and decreasing to zero in the leaf senescence stage. Annual ETc trend during the three growing was very similar, which was explained by the different growing periods and the LAI variation. In the 1st year Kc was 0.47 for both treatments. In the 2nd, 3rd, and 4th years Kc ranged from 0.15 to 1.38 for center pivot treatment and from 0.15 to 1.25 for drip treatment. Kc average in 2nd, 3rd, and 4th years during vegetative and productive growing periods was 0.77, 0.93, and 0.82 for center pivot treatment, respectively, and 0.69, 0.79, and 0.74 for drip treatment, respectively. The relationship between Kc and LAI for the center pivot treatment was adjusted to a logarithmical equation with coefficient of determination (R2) and root mean square error (RMSE) of 0.7643 and 0.334, respectively. For the drip treatment R2 was 0.8443 and 0.2079, respectively. In all three years analyzed, Kc related to CTU by a 3rd degree polynomial equation for both treatments.
A determinação de coeficiente de cultivo (Kc) com metodologia adequada é essencial para quantificar o consumo hídrico de cultivos em diferentes regiões. Valores de Kc do pinhão-manso (Jatropha curcas L.) ainda não foram determinados e essa informação é muito importante para auxiliar o manejo de irrigação de maneira adequada. O objetivo desse estudo foi determinar a evapotranspiração (ETc) e Kc do 1º ao 4º ano de cultivo do pinhão-manso, e correlacionar Kc com o índice de área foliar (IAF) e a soma da unidade térmica (SUT). O experimento foi realizado de março de 2012 à agosto de 2015 na Escola Superior de Agricultura \"Luiz de Queiroz\" (ESALQ)/Universidade de São Paulo (USP), na cidade de Piracicaba, SP, Brasil. O experimento foi divido nos tratamentos irrigados por pivô central, gotejamento e sem irrigação. Foram utilizados dois lisímetros de pesagem (12 m2 de superfície em cada lisímetro) por tratamento para realizar a determinação de ETc (uma planta por lisímetros). A evapotranspiração de referência (ET0) foi determinado pelo método de Penman-Monteith a partir de dados meteorológicos coletados na estação meteorológica localizada ao lado do experimento. Valores diários de Kc foram determinados nos tratamentos irrigados pela razão entre ETc e ET0 (Kc=ETc/ET0). IAF foi determinado utilizando o equipamento LAI-2200 Plant Canopy Analyzer, que foi previamente calibrado para adequar as características do dossel do pinhão-manso. Em todos os anos avaliados, o IAF foi quase zero durante o início do período vegetativo, aumentando os valores conforme a planta começou a se desenvolver até atingir valores máximos durante o período produtivo, decrescendo os valores até zero no estádio de desenvolvimento de senescência foliar. A variação anual de ETc no 2º, 3º e 4º ano foi muito similar, explicado pelos diferentes períodos de desenvolvimento da cultura e a variação de IAF no ano. No 1º ano, Kc foi 0,47 para os dois tratamentos irrigados. No 2º, 3º e 4º ano, Kc variou de 0,15 a 1,38 no tratamento irrigado por pivô central e de 0,15 a 1,15 no tratamento irrigado por gotejamento. A média dos valores de Kc no 2º, 3º e 4º ano durante os períodos vegetativos e produtivos foi de 0,77, 0,93 e 0,82 no tratamento irrigado por pivô central, respectivamente, e 0,69, 0,79 e 0,74 no tratamento irrigado por gotejamento, respectivamente. A relação entre Kc e IAF mostrou, para o tratamento irrigado por pivô central, um ajuste logaritmo com coeficiente de determinação (R2) e somatória do erro médio ao quadrado (SEMQ) de 0,7643 e 0,334, respectivamente, e 0,8443 e 0,2079 para o tratamento irrigado por gotejamento, respectivamente. Nos três anos analisados, Kc correlacionado com SUT mostrou o melhor ajuste à equação polinomial de 2ª ordem para os dois tratamentos.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The increased interest in biofuels in recent years, coupled with the problem of the need for environmental sanitation in different areas of the economy justify the proposal of this work, which is the reuse of wastewater from swine (SW) in the jatropha crop (Jatropha curcas L.) and its influence on oil acidity - short parameter importance in the production of biodiesel; culture stands out from oilseeds for its oil production capacity and high adaptability in different scenarios. The experiment featured a Jatropha plantation area of 900 m², which were used 216 m² for conducting the experiment, divided into eighteen installments, the research was conducted over three years; each year the plots received six treatments with different proportions of SW with three repetitions each, applications were performed in triplicate with an interval of three months between each application. Seed harvest was done by hand during the entire production plan period from the first application of each year, the oil was extracted with solvent (ethanol), justified because it is cheaper, less toxic and less harmful to the environment, when compared to other organic solvents such as hexane. The acidity was determined by titration process with sodium hydroxide (NaOH) at the end of each experimental cycle; further the composition was determined as the percentage of oils of fatty acids (FA) by gas chromatography. The results showed an inversely proportional reduction of acidity amount of SW applied during the three years analyzed; It was also observed a decrease in linolenic acid concentration due to the increase of the applied dose and in all cycles. Knowing FA unsaturated oils leave them more susceptible to oxidation, reduction FA that may be related to decreased acidity
O aumento do interesse por biocombustíveis nos últimos anos, somado à problemática da necessidade do saneamento ambiental em diferentes áreas da economia, justificam a proposta do presente trabalho, que é o reuso da água residuária da suinocultura (ARS) na cultura do pinhão manso (Jatropha curcas L.) e sua influência sobre a acidez do óleo - parâmetro de suma importância na produção de biodiesel; a cultura se destaca dentre as oleaginosas por sua capacidade de produção de óleo e a alta adaptabilidade em diferentes cenários. O experimento contou com uma área de plantio de pinhão manso de 900 m², da qual foram utilizados 216 m² para condução do experimento, divididos em dezoito parcelas, a pesquisa foi conduzida ao longo de três anos; em cada ano as parcelas receberam seis tratamentos com diferentes proporções de ARS com três repetições cada, as aplicações foram realizadas em triplicata com intervalo de três meses entre cada aplicação. A colheita das sementes foi feita manualmente durante todo o período produtivo da planta a partir da primeira aplicação de cada ano, o óleo foi extraído com solvente (etanol), justificado pelo fato de ser mais barato, menos tóxico e menos nocivo ao meio ambiente, quando comparado a outros solventes orgânicos, como o hexano. A acidez foi determinada pelo processo de titulação com hidróxido de sódio (NaOH) ao final de cada ciclo experimental; foi determinada ainda a composição dos óleos quanto a porcentagem de ácidos graxos (AG), por cromatografia gasosa. Os resultados mostraram uma redução da acidez inversamente proporcional a quantidade de ARS aplicada, nos três anos analisados; observou-se ainda um decréscimo na concentração do ácido linolênico em função do aumento das doses aplicadas e em todos os ciclos. Sabendo que AG insaturados deixam os óleos mais susceptíveis à oxidação, a redução desse AG pode estar relacionada à diminuição da acidez

Uppsatsen syftar till att undersöka ungdomars reception av fenomenet manga/anime på ett etnografiskt arbetssätt, avgränsat med begreppet ungdomskultur. Fokus ligger på två mangayttringar som närmast berör ungdomar: shōjo för flickor och shōnen för pojkar. Resultatet redovisas i tre olika teman: Estetik/Stil, Makt/Könsroller och Berättelser/Myter,vilka tolkas med hjälp av ett raster på textuell, kontextuell och sociohistorisk nivå.Undersökningen är kvalitativ med sju ungdomar som informanter, och empirin består avfältanteckningar, fotodokumentation och intervjuer.Ungdomarnas fascination av mangaserier kan delvis förklaras med att de ritas i ett utstuderat ”filmiskt” maner, med snabba bildväxlingar och många olika bildvinklar, och delvis med att läsaren kan följa protagonistens utveckling under många år, parallellt med sin egen utveckling till ett vuxnare jag. Kulturyttringen uppmuntrar även till eget medskapande och egenamatörmanga-produktion, vilket medför att många ungdomar publicerar sitt eget material,både i pappersform och på internet. Detta gör fenomenet förhållandevis ohierarkiskt.Manga/anime-traditionen är ofta ironisk och lekfull, motiven i den tidiga sekventiellabildkonsten i Japan hämtades från nöjes- och teatervärlden, men under den flamsiga ytan döljsofta allvarliga resonemang. Berättartraditionen kan uppfattas utifrån tre olika modus: vardagligt, humoristiskt och poetiskt. Ungdomarna kan i postmodernistisk anda ta del av det främmande och egendomliga, men kan också; när de själva vill, stänga av och avskärma sigifrån det.Estetiken betecknas av eklekticism, brottstycken, lekfull inställning till form och konventionoch raserandet av hierarkiska traditionerna. Könsroller utmanas med crossdressing, och demanliga protagonisterna ritas ofta med påfallande feminina anletsdrag. Uppsatsen beskriverdetta med bakgrund av queerteoriska tankegångar, där en given norm ifrågasätts. Läsandet avserietidningar har nästan alltid betraktats nedlåtande, med någon form av moralpanik i sitt kölvatten. Detta kan betraktas som en reaktion mot en pågående modernisering. Resultatet av uppsatsen visar inte på någon större förändring i det traditionella könsrollstänkandet, men en viss omformulering pågår.

This thesis has taken part as an experimental development within the Learning Ecology through Science with Global Outcomes project. It introduces the area of cross platform mobile application development and provides a possible solution for tackling the current fragmentation of mobile devices and platforms for data collection. During the process, a mobile data collection prototype was designed, implemented and deployed on Android, iOS and Windows Mango by using standards and web standards such as HTML5, CSS3, XForms and JavaScript. The deployed prototype was then tested with users in order to collect the necessary data to help answer the questions that were formulated. The results indicate an ease of use of the prototype in relation to cross platform development and also shows potential benefits such as less code and time. Cross platform development provides a way to counteract the current fragmentation between mobile platforms.

O conhecimento da demanda hídrica de uma cultura é fundamental para o planejamento da implantação de novas áreas, bem como para a análise da viabilidade econômica de uma cultura em determinada região. A utilização de culturas para fontes de matéria prima alternativa vem sendo altamente estudada nos últimos anos. Dentre muitas, vem se destacando no cenário nacional e mundial a cultura do pinhão-manso (Jatropha curcas L.) para a produção de biodiesel. O consumo de água por parte do pinhão-manso é pouco conhecida, necessitando ser estudada a fim de aprofundar os conhecimentos sobre a cultura e fornecer parâmetros confiáveis para a prática da irrigação. Os objetivos desse trabalho foram determinar a evapotranspiração (ET), a evaporação (E), a transpiração (T), o coeficiente de cultivo (Kc), coeficiente de evaporação (Ke) e coeficiente de cultivo basal (Kcb) do pinhão-manso na fase de formação irrigados por pivô central, gotejamento e sem irrigação na cidade de Piracicaba, SP. O experimento foi realizado na Fazenda Areão da Escola Superior de Agricultura \"Luiz de Queiroz\" da USP durante o período de março de 2012 a abril de 2013. O experimento foi dividido em três tratamentos, sendo: irrigado por pivô central, gotejamento e sem irrigação. A determinação de ET foi realizada por meio lisímetros de pesagem em cada tratamento, contabilizando a variação de massa em função das entradas saídas de água no sistema. A determinação do Kc foi realizada pela razão entre ET e ETo (estimado a partir de dados coletados na estação meteorológicos automática da Fazenda Areão). Por meio de microlisímetros de pesagem, foi determinado E. De posso dos dados de E, foram determinados os valores de T pela diferença de E (determinado no microlisímetro) e ET (determinado no lisímetro). Os valores de Ke e Kcb foram determinados pela razão de E e T por ETo, respectivamente. Os valores de ET variaram, principalmente, em função do método de irrigação. Foi observado uma superioridade dos valores de ET para o tratamento irrigado por pivô central, seguido do tratamento irrigado por gotejamento e sem irrigação. As médias dos tratamentos durante o primeiro ano foram 3,17, 2,82 e 2,82 mm dia-1 para os tratamentos irrigado por pivô central, irrigado por gotejamento e sem irrigação, respectivamente. Essa mesma proporção de ET entre os tratamentos irrigados foi observado nos valores de Kc. A média do período de Kc foi 0,69 e 0,63 para os tratamentos irrigados por pivô central e gotejamento. Essas maiores taxas de ET e Kc do tratamento irrigado por pivô central em relação ao tratamento irrigado por gotejamento foi devido ao método de irrigação por gotejamento ser mais eficiente na utilização de água pelas plantas em relação ao método por pivô central. Nesse método, a água é aplicada de maneira localizada as plantas, diferentemente do método por pivô central, em que a água é aplicada em toda a superfície do solo. Isso faz com que as taxas de E no método por gotejamento seja menor em relação ao pivô central, diminuindo as taxas de ET. De maneira geral, as taxas de E e Ke foram maiores em relação aos valores de T e Kcb durante o período analisado. Isso foi devido as plantas estarem com porte baixo e o solo estar quase por completo exposto as variações atmosféricas, fazendo com que as taxas de E e Ke sejam superiores as de T e Kcb. A média de E e Ke foram 1,85 mm dia-1 e 1,03, respectivamente, e as médias de T e Kcb foram 0,4 mm dia-1 e 0,2, respectivamente.
The knowledge of the water demand of a culture is essential for planning of new areas, as well as for the economic viability analysis of a culture in a specific region. The use of crops for alternative feedstock sources has been extensively studied in the last years. Among many, jatropha (Jatropha curcas L.) has been highlighted for biodiesel production in the national and global scenarios. Water consumption by the jatropha is little known, which requires to be studied in order to increase the knowledge and provide reliable parameters for the irrigation practice. The objectives of this study were to determine the evapotranspiration (ET), evaporation (E), transpiration (T), crop coefficient (Kc), evaporation coefficient (Ke) and basal crop coefficient (Kcb) of jatropha in the formation phase irrigated by center pivot, drip and without irrigation in Piracicaba, SP. The experiment was conducted at Areão Farm of Escola Superior de Agricultura \"Luiz de Queiroz\" of USP during March 2012 to April 2013. The experiment included three treatments: center pivot irrigated, drip irrigated and dryland. The determination of ET was made by weighing lysimeters for each treatment, accounting the inputs and outputs of water in the lysimeter system. The determination of Kc was made by the ratio between ET and ETo (estimated from data collected at the automatic meteorological station of Areão Farm). The determination of E was made by microlysimeters and T was determined between the difference of ET and E. Ke and Kcb values were determined by the ratio of E and T by ETo, respectively. ET values varied, mainly, according to the irrigation method. It was observed superiority of ET values for the treatment irrigated by center pivot, followed by the treatment irrigated by drip and without irrigation. The average during the first year was 3.17, 2.82 and 2.82 mm day-1 for the treatment irrigated by center pivot, irrigated by drip irrigated and without irrigation, respectively. The same proportion of ET from irrigated treatments was observed in the values of Kc. In the period, the average of Kc was 0.69 and 0.63 for the treatments irrigated by center pivot and irrigated by drip. These higher rates of ET and Kc of the treatment irrigated by center pivot compared to the treatment irrigated by drip was due to the method of drip irrigation is more efficient in water use by plants in relation to the method irrigated by central pivot. In this method, the water application is made in a localized way, differently in relation to the center pivot method in which water is applied all over the soil surface. This makes E rates given by drip irrigation method lower than the center pivot irrigation method, decreasing its ET averages. In general, rates of E and Ke were higher compared T and Kcb values during the period analyzed. This was explained due the short plants stature and the soil was almost completely exposed to atmospheric variations, causing rates of E and Ke exceed from T and Kcb rates. The average of Ke and E were 1.85 mm day-1 and 1.03, respectively, and the average of T and Kcb were 0.4 mm day-1 and 0.2, respectively.

Nos últimos anos tem aumentado o interesse no uso do pinhão-manso (Jatropha curcas L.) como fonte de óleo para a produção de biodiesel. No entanto, o conhecimento atualmente disponível sobre o consumo hídrico desta cultura é escasso e constitui um dos principais fatores limitantes no seu uso como alternativa agroenergética. Somado a isso, acredita-se que o aumento da produção de biodiesel no mundo irá aumentar a pressão sobre os recursos hídricos, o que torna necessário utilizar a água na agricultura da forma mais eficiente possível. Assim, este estudo teve como objetivo construir seis lisímetros de pesagem direta que serão utilizados para estudar o consumo hídrico do pinhão-manso irrigado por pivô central, gotejamento e sem irrigação. Esses lisímetros foram calibrados e testados quanto a sua sensibilidade a temperatura do ar e vento. Além disso, comparou-se a influência do uso de duas células de carga diferentes na qualidade dos mesmos. Na área de cada tratamento foram instalados dois lisímetros, sendo um utilizando células de carga ALFA® e outro HBM®. Cada lisímetro foi constituído por tanques de aço carbono de 1,955 m de raio interno (12 m2) e 1,3 m de profundidade útil. Estes foram circundados por paredes de concreto, possuíam sistema de drenagem e se encontravam diretamente apoiados sobre três células de carga. Ambos os modelos das células apresentavam capacidade nominal de 10.000 kg, sendo a capacidade combinada do lisímetro de 30.000 kg. Os lisímetros foram calibrados adicionando e, depois, retirando 1.000 kg de massa dos mesmos. Os dados foram usados para ajustar um modelo de regressão linear entre a massa acumulada e a média do sinal de saída das três células de carga. No teste de sensibilidade à temperatura, a massa dos lisímetros foi monitorada por até dois dias consecutivos, sob condição de massa constante e com proteção para não haver interferência do vento. Os dados foram comparados com a temperatura registrada na estação meteorológica e, também, com a temperatura medida pelo datalogger e um termohigrômetro, os quais foram instalados no fosso dos lisímetros. Para a avaliação da sensibilidade ao vento, foram construídos protótipos de árvores usando tubos de PVC e lonas de ráfia. Em cada lisímetro instalou-se uma árvore e, sob condição de massa constante, a massa dos mesmos foi monitorada por seis dias consecutivos. Os dados obtidos foram comparados com a velocidade do vento medida na estação meteorológica. Todos os lisímetros construídos neste estudo apresentaram qualidade suficiente para serem usados na determinação da evapotranspiração em escala horária e diária. Nos lisímetros ALFA®, a resolução final das medidas foi de 0,0013 mm, sendo realizadas com boa precisão, apresentando histerese e baixa acurácia (erro padrão entre 0,27 e 1,04 mm). Os lisímetros HBM® apresentaram resolução final de 0,00088 mm, boa precisão, pouca ou nenhuma histerese e alta acurácia (erro padrão entre 0,03 e 0,1 mm). Todos os lisímetros demonstraram não sofrer influência da temperatura e do vento na determinação da evapotranspiração, embora, em ambos, ALFA® e HBM®, a precisão das medidas foi reduzida linearmente com o aumento da velocidade do vento.
Recently, there is an increasing interest in using physic nut (Jatropha curcas L.) as a source of oil for biodiesel production. However, currently knowledge regarding its crop water requirement is scarce and constitutes one of the main gaps that limit its use as an agroenergy alternative. In addition, there is a general agreement that an increase in biodiesel production worldwide will bring additional pressure to water resources, what provokes to use water in agriculture in a more efficient way. So, this study had as objective to construct six direct weighing lysimeter that will be used to study the water use of physic nut conducted under sprinkler and drip irrigation, and without irrigation. These lysimeters were calibrated and tested for its sensibility to air temperature and wind. Furthermore, there was also compared the influence of using two different load cells on their quality. Two lysimeters were installed for each treatment, one using ALFA® load cells and other using HBM®. Each lysimeter was constituted by a steel tank with 1.955 m inner radius (12 m2) and 1.3 m of usable depth. They were encompassed by concrete walls, have a drainage system and were directly supported by three load cells. Both load cell models has a nominal capacity of 10,000 kg, with a lysimeter summed capacity of 30,000 kg. Lysimeters calibration procedure was done by adding and, after it, removing 1,000 kg of mass from then. Data were used to fit a linear regression model between the accumulated mass and the mean output value of the three load cells. For testing the sensibility to temperature, lysimeters mass were monitored till two consecutive days, under constant mass and with a barrier for protecting against wind interference. Data were compared to the temperature measured by the weather station and, also, by that measured by a datalogger and a thermohygrometer, which were installed inside the lysimeters pit. For testing to wind sensibility, there were constructed some trees prototypes using PVC pipes and raffia tarpaulin. One tree was installed on each lysimeter and, under constant mass, lysimeters mass were monitored during six consecutive days. Data were compared to the wind velocity, as measured by the weather station. All the six lysimeters constructed in this study presented adequate quality to be used for the determination of evapotranspiration on hourly and daily basis. For ALFA® lysimeters, the final measurement resolution was 0.0013 mm, being obtained with good precision, hysteresis, and low accuracy (standard error of 0.27 to 1.04 mm). HBM® lysimeters presented a final resolution of 0.00088 mm, good precision, few or no hysteresis, and high accuracy (standard error between 0.03 and 0.1 mm). All lysimeters proved to be not influenced by air temperature and wind on the determination of evapotranspiration, in spite of, in both, ALFA® and HBM® lysimeters, their measurement precision was linearly reduced with an increase in wind velocity.

Objetivo general • Definir y explicar los fenómenos de Narrativa cross-media a escala global, en especial, aquellos relacionados con la industria japonesa del entretenimiento y sus principales productos: manga, anime y videojuegos. La relación entre estos productos y su expansión mediática en forma de media-mixes será estudiada a través de diferentes herramientas teóricas y su aplicación a casos específicos de la industria cultural japonesa. METODOLOGÍA • Revisión histórica de los principales autores e investigadores sobre Narrativa, Transmedia Storytelling, Manga, Anime y cultura japonesa desde diferentes perspectivas teóricas (Teoría literaria y de géneros, sociología política, historia del arte, film studies, etc.). • Caso de estudio con aplicación del marco teórico desarrollado RESULTADOS / CONCLUSIONES Para el análisis del macro-relato de Full Metal Alchemist (Square Enix, 2001-2011) se ha desarrollado una metodología fundamentada en varios ejes de análisis. • En la primera de ellas se ha caracterizado la NC de la franquicia como una arquitectura reticular centralizada que sigue la tradición de otras franquicias del manganime japonés. Se analizan así mismo otras características de la intertextualidad del macro-relato, en función de su relación con los géneros shōnen y CF. • Seguidamente, se ha utilizado el concepto de Estructura Profunda para definir qué elementos son imprescindibles en la construcción de mundos narrativos. En ese sentido se ha adoptado una visión desestructurada –ecléctica si se prefiere- de la narrativa. La narrativa es por tanto no una cualidad monolítica sino un prototipo por cuya aproximación definimos diferentes actos como poseedores o no, de cierto grado de narratividad. • Una vez definidas aquellas cualidades que consideramos esenciales de la narrativa, según los diferentes marcos teóricos y adaptados al contexto de las franquicias cross-media japonesas, hemos incorporado a nuestro análisis otras herramientas narratológicas con el propósito de relacionar el producto narrativo con una estructura superior de comunicación.
OBJECTIVES To define and explain the phenomena of Cross-Media Narrative on a global scale, particularly those related to the Japanese entertainment industry and its main products: manga, anime and videogames. The relationship between these products and their diffusion through media in the form of media-mixes will be studied by means of different theoretical tools and their application to specific cases in the Japanese cultural industry. METHODOLOGY • Historical review of main studies about Transmedia Storytelling, Manga, Anime and Japanese Culture from different perspectives (Literature and genre Theories, political sociology, Art and Film Studies, etc.). • Case of study as an implementation of a theoretical framework MAIN RESULTS For the analysis of the macro-tale of Full Metal Alchemist Franchise (Square Enix, 2001-2011) a methodology has been developed founded on some central areas of analysis. • In the first of these the Cross-Media Narrative of the franchise has been characterized as a reticular centered architecture that follows the traditions of other franchises of Japanese manganime. In this way other characteristics of intertextuality of the macro-tale are analyzed, based on their relationship with shōnen and SF genres. • Next, the concept of “Deep Structures” has been used to define the elements as indispensable in the construction of “storyworlds”. In this sense, an unstructured vision has been adopted –eclectic if preferable– of the narrative. The narrative is not, therefore, a monolithic quality but a prototype for whose approximation we define different acts as possessor or no, of a certain “degree of narrativity”. • Once these qualities that we consider essential of narrative are defined, following the different theoretical frameworks and adapted to the context of the Japanese cross-media franchise, we have incorporated in our analysis other narrative tools with the purpose of relating the narrative product with a higher communication structure

JEREISSATI, Camila Barbosa Pinheiro. Análise proteômica de plastídeos do endosperma de sementes em desenvolvimento de pinhão manso (Jatropha curcas L.). 2015. 127 f. Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza, 2015.
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Jatropha curcas L. is a plant native to America and belongs to the Euphorbiaceae family. Currently it is gaining economical interest mainly because it is an oilseed crop with potential to produce biodiesel. However, presence of phorbol esters (a class of diterpenes) that are the major toxic constituents of the seeds, limits a better usage of the plant, by making the use of the residue, obtained after the oil extraction from the seeds, unfeasible for animal feed, due to its pro-carcinogenic activity and inflammatory action. Proteomic analysis of the plastids isolated from developing seeds of Jatropha is important because the synthesis of fatty acid as well as phorbol esters, the two most attractive compounds in the study of Jatropha curcas, occur in plastids. Proteomic analysis of this organelle is crucial to better understand and explore not only the biosynthetic pathway of these two compounds but other metabolic pathways , and addtionaly providing foundation for researchs that aimed to develope genotypes with more suitable characteristics for industrial applications. In this study, we performed a proteomic analysis of plastids isolated from the endosperm of developing Jatropha curcas seeds that were in the initial stage of deposition of protein and lipid reserves. Proteins extracted from the plastids were digested with trypsin, and the peptides were applied to an EASY-nano LC system coupled online to an ESI-LTQ-Orbitrap Velos mass spectrometer, and this led to the identification of 1103 proteins representing 804 protein groups, of which 923 were considered as true identifications, and this considerably expands the repertoire of J. curcas proteins identified so far. Of the identified proteins, only five are encoded in the plastid genome, and none of them are involved in photosynthesis, evidentiating the nonphotosynthetic nature of the isolated plastids. Homologues for 824 out of 923 identified proteins were present in three different plastids proteins databases i.e. PPDB, SUBA and PlProt, while homologues for 13 proteins were not found in any of these three databases but were marked as plastidial by at least one of the three prediction programs used (TargetP, ChloroP and PlantMPloc). Functional classification showed that proteins belonging to amino acids metabolism comprise the main functional class, followed by carbohydrate, energy, and lipid metabolisms. The small and large subunits of Rubisco were identified, and their presence in plastids is considered to be an adaptive feature counterbalancing for the loss of one-third of the carbon as CO2 as a result of the conversion of carbohydrate to oil through glycolysis. While several enzymes involved in the biosynthesis of several precursors of diterpenoids were identified, we were unable to identify any terpene synthase/cyclase, which suggests that the plastids isolated from the endosperm of developing seeds do not synthesize phorbol esters. In conclusion, this study provides insights into the major biosynthetic pathways and certain unique features of the plastids from the endosperm of developing seeds at the whole proteome level.
O pinhão manso (Jatropha curcas L.) é uma planta nativa da América, pertencente à família Euphorbiaceae. Atualmente, ela desperta interesse econômico principalmente por se tratar de uma oleaginosa com potencial para a produção de biodiesel. Entretanto, a presença de ésteres de forbol (uma classe de diterpeno), que são os principais constituintes tóxicos das sementes, limita uma melhor utilização dessa planta, por inviabilizar o uso do resíduo de extração do óleo das sementes na alimentação animal, bem como, por apresentar atividade pró-carcinogênica e ação inflamatória. A análise proteômica de plastídeos, isolados de sementes em desenvolvimento de pinhão manso, é uma importante vertente de estudo, pois tanto a síntese de ácidos graxos como dos ésteres de forbol, os dois compostos mais atrativos no estudo de Jatropha curcas, ocorrem nos plastídeos. O estudo proteômico dessa organela torna-se crucial para melhor compreender e explorar não somente as vias biossintéticas desses dois compostos, como de outras vias metabólicas, além de proporcionar um conjunto de dados que pode ser utilizado em pesquisas voltadas para o desenvolvimento de genótipos com características mais adequadas para aplicações industriais. No presente trabalho, realizou-se uma análise proteômica de plastídeos isolados do endosperma de sementes em desenvolvimento do pinhão manso, que estavam nos estágios iniciais de deposição de lipídios e proteínas de reserva (25-30DAA), confirmados por meio de análises histológica e histoquímica. As proteínas extraídas dos plastídeos foram digeridas com tripsina e os peptídeos foram aplicados no sistema de nano-LC EASYII acoplado online ao espectrômetro de massa nano ESI LTQ-Orbitrap velos, o que resultou na identificação 1103 proteínas, representando 804 grupos de proteínas, dos quais 923 foram consideradas identificações verdadeiras. Isso expandiu consideravelmente o repertório de proteínas do pinhão manso até agora identificas. Dentre as proteínas identificadas, apenas 5 são codificadas pelo genoma plastidial, e nenhuma delas está envolvida na fotossíntese, o que evidencia a natureza não fotossintética dos plastídeos isolados. Homólogos de 824, dentre as 923 proteínas identificadas, estavam presentes nos bancos de dados PPDB, SUBA e PlProt, enquanto homólogos para 13 proteínas não foram encontrados em nenhum dos três bancos de dados plastidiais, mas foram detectados como plastidiais por pelo menos um dos três programas de predição de localização subcelular utilizados (TargetP, ChloroP, PlantMPloc). A classificação funcional mostrou que a maioria das proteínas identificadas pertencia ao metabolismo dos aminoácidos, seguido dos metabolismos dos carboidratos, energético e dos lipídios. As subunidades maiores e menores da Rubisco foram identificadas, e sua presença nos plastídeos foi considerada uma característica adaptativa para contrabalancear a perda de um terço do carbono na forma de CO2 como um resultado da conversão de carboidratos em óleo através da glicólise. Apesar de enzimas envolvidas na biossíntese de diversos precursores dos diterpenóides terem sido identificadas, não foi detectado nenhuma terpeno sintase/ciclase, o que sugere que os plastídeos isolados do endosperma de sementes em desenvolvimento não sintetizam ésteres de forbol, apesar de uma grande quantidade desse composto ser encontrada neste tecido. Como conclusão, o presente trabalho proporciona insights sobre as principais vias biossíntéticas, e sobre características peculiares dos plastideos isolados do endosperma de sementes em desenvolvimento.

This study was conducted in the community of Yampupata, in the municipality of Copacabana, in the Manco Kapac province, La Paz, to an altitude of 3841 m.a.s.l, agricultural campaign 2003-2004. The purpose of the study was to determine and compare the fenological stages (germination, growth, leafing, and harvesting), hypocotyl yield (dry and fresh), classification of the hypocotyl (diameter, length, etc.), and the economical evaluation as well. The experiment set up was installed in the lower region of the community, dedicated to agriculture, performing all the aforementioned activities together for the period of a year. The experimental design was of Random blocking test, having three types of Maca (lepidium mevenni Walp.): yellow, purple, and cream , five levels of ovine manure ( 0.0, 2.5, 7.5, and 10.0 tn/ha), distributed in the four blocks, 15 treatments, 60 experimental units, with a planting density of 3 kg/ha, and a total area of 300 m². According to the results, it is recommended to plant the cream, and the yellow ecotype of Maca, due to their positive development under the conditions of the soil, and climate of the zone. The cream ecotype under the effect of 7.5 tn/ha of ovine manure (a3b3) had a yield of 8055.06 kg/ha in 238 days (8 months) , with a rentability index of 3.75. In comparison, the purple ecotype with a 0.0 tn/ha of ovine manure (a2b1) had a yield of 4319.55 kg/ha, and a rentability index of 1.74 in eight months. The cream ecotype with 7.5 tn/ha of ovine manure, presented the best behavior under local conditions (climate, soil, and handling), the yield and rentability index were superior to the other treatments; therefore, it is recommended to implement a production plan in the sector according to the results obtained from the study.

Com a crescente procura por alternativas para suprir energeticamente o mundo, cada vez mais, fontes mais limpas e renováveis estão sendo utilizadas. No Brasil, a produção de óleos vegetais visando a geração de energia (biodiesel) ainda é incipiente. Dentre as diferentes espécies de oleaginosas, o pinhão manso (Jatropha curcas L.) vem se destacando como uma ótima alternativa no fornecimento de matéria prima para a produção de biodiesel. Informações sobre essa cultura ainda são escassas, principalmente no Brasil, onde há pouco tempo foram iniciadas as pesquisas. A necessidade de informações locais também ressalta a importância da pesquisa com o Pinhão Manso. Com base nessas necessidades, o objetivo desse experimento foi analisar a viabilidade técnica da produção de mudas irrigadas de pinhão manso por sistema de subirrigação e desenvolver um sistema automatizado de manejo. O controle da irrigação foi realizado a partir da variação de peso dos tubetes com as mudas, devido a perda de água por evapotranspiração. No processo de controle dos pesos foram utilizadas células de carga, que monitoraram a variação de peso de um conjunto de tubetes denominado conjunto controle. O delineamento experimental foi em delineamento inteiramente aleatorizado, com quatro repetições. Os tratamentos avaliados foram quatro níveis de depleção da máxima capacidade de retenção de água (MCRA) apresentada pelo substrato (T1=20%, T2=40%, T3=60% e T4=80% da MCRA). Para se quantificar os efeitos dos tratamentos foram avaliadas semanalmente as seguintes características das mudas: altura e diâmetro do caule, número de folhas e área foliar, bem como a produção de matéria fresca e seca da parte aérea e do sistema radicular. Os resultados mostram que o tratamento 2 foi o que se destacou dentre os demais, produzindo mudas de melhor qualidade, com maior acúmulo de matéria seca e maior consumo hídrico.
With the growing demand for alternative energy to meet the world, increasingly more clean and renewable sources are being used. In Brazil, the production of vegetable oils aimed at producing energy (biodiesel) is still incipient. Among the different species of oleaginous plants, jatropha (Jatropha curcas L.) has emerged as a great alternative in providing raw material for biodiesel production. Information on this crop are still scarce, especially in Brazil where they recently started research. The need for local information also emphasizes the importance of research with Jatropha. Based on these needs, the aim of this experiment was to analyze the technical viability production of jatropha seedlings irrigated by subirrigation system and develop an automated system management. The irrigation control was carried out from the weight variation of the vials with the seedlings because of water loss through evapotranspiration. In the process of weight control were used load cells, which monitored the weight variation of a set of vials called the control group. The experimental design was the completely randomized with four replications. The treatments were four levels of depletion of the maximum water holding capacity (MCRA) presented by the substrate (T1=20%, T2=40%, T3=60% e T4=80% da MCRA). To quantify the effects of the treatments were evaluated weekly the following characteristics of seedlings: height and diameter of the stem, leaf number and leaf area, and the production of fresh and dry shoot and root. The results show that treatment 2 was what stood out among the others, producing higher quality seedlings with greater dry matter accumulation and increased water consumption.

Individuals interacting in an environment that exacerbates fear of crime and general distrust may face erosion of democratic values and perceive authoritarian policies as a solution to restore order. In Latin America historical widespread distrust in the government apparatus as well as fear of crime, have always been a topic of interest, not only for sociologists but also for political scientists and lawmakers. This study uses the LAPOP wave 2012 (Latin American Public Opinion) survey to assess Ecuadorians’ perceptions about trust in the government and fear of crime as predictors of support for authoritarian policies (mano dura). Logistic regressions show evidence that fear of crime acts as a better predictor than level of trust in the government for predicting the likelihood of supporting authoritarian policies. Overall, this study provides a hint of how Ecuadorians support for democratic principles may have weakened by fear of crime and lack of trust in the government apparatus.

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
The purpose of this thesis was to investigate the feasibility of using the honey bee (Apis mellifera L.) for pollination and yield increment in Jatropha curcas L. crops. Therefore, the floral biology and pollination process of J. curcas were studied as well as the foraging behavior and pollination efficiency of A. mellifera in this crop by the introduction of four colonies in a hectare. The work was carried out in a five years-old commercial plantation comprising 10ha, in the state of PiauÃ, (08Â32â19,0âS and 43Â56â19,7âW, 220 meters above sea level), Brazil, from March to July, 2009. Flowers were presented in protogynous inflorescences and the anthesis and most of pollen presentation took place mainly in the morning shift. Inflorescences last, in average, 20 days, with female flowers concentrating in the first third of the period and male flowers predominating in the second third, in a relation of 18.1:1 male/ female flower. Although this may favor xenogamy, the stigma receptivity lasts for, at least, five days ensuring the flower to reach the period of most pollen release in its own inflorescence, facilitating the occurrence of geitonogamy. Flowers pollinated up to four days after anthesis did not differ (p>0.05) in fruit setting to those pollinated in the first, second or third day after anthesis. Foraging by A. mellifera occurs throughout the day and peaks between 13h00min and 15h00min and is characteristic of nectar harvesting both in male and female flowers. Only one visit by A. mellifera to J. curcas flowers set 100% producing results similar (p>0,05) to those from manual xenogamy (96%), manual geitonogamy (94%) and open pollination (93%). However, only one visit by a bee was not enough to maximize the oil content per seed (213mg) in comparison to the open pollination and geitonogamy treatments (both 250mg). Besides that, it was possible to observe that geitonogamy led to greater oil production than xenogamy (237mg). It is concluded that A. mellifera is an efficient pollinator of this crop. The introduction of four honey bee colonies per hectare maximize oil production and produce the best results for all parameters studied. Furthermore, only one visit by the honey bee to J. curcas flowers was enough to prevent pollination deficit in this crop.
O objetivo desta tese foi investigar a possibilidade da utilizaÃÃo de Apis mellifera L. na polinizaÃÃo e incremento de produtividade da cultura do pinhÃo manso (Jatropha curcas L.). Para tanto, foi estudado a biologia floral e a polinizaÃÃo do pinhÃo manso bem como o comportamento forrageiro e a eficiÃncia polinizadora de Apis mellifera. O trabalho foi realizado em 10ha de plantio comercial jà estabelecido, com cinco anos de idade, no estado do PiauÃ, (08Â32â19,0âS e 43Â56â19,7âW e 220 metros de altitude), entre os meses de marÃo e julho de 2009. A antese das flores do pinhÃo manso, distribuÃdas em cimos com carÃter protogÃnico, ocorre basicamente no perÃodo matinal, bem como a maior oferta de pÃlen. As inflorescÃncias duram, em mÃdia, 20 dias, com maior concentraÃÃo de flores femininas no terÃo inicial e de masculinas, no terÃo mÃdio, com relaÃÃo de 18,1 flores masculinas para cada feminina. Embora esse fato favoreÃa a xenogamia, a receptividade do estigma por, pelo menos, cinco dias, garante a flor alcanÃar o perÃodo de maior oferta de pÃlen no prÃprio cimo, oportunizando a geitonogamia. As flores polinizadas atà o quarto dia frutificam da mesma forma que aquelas polinizadas no primeiro, segundo e terceiro dias apÃs antese, nÃo havendo diferenÃas significativas. O forrageamento por A. mellifera ocorre durante todo o dia, com maior pico entre 13h00m e 15h00m e à tÃpico de coleta de nÃctar, tanto nas flores masculinas quanto nas femininas. Apenas uma visita da abelha Ãs flores acarreta em 100% de vingamento, mostrando resultado semelhante, (p>0,05), aos tratamentos de xenogamia manual (96%), geitonogamia manual (94%), e polinizaÃÃo aberta (93%). PorÃm, uma visita apenas, nÃo à suficiente para produzir maior quantidade de Ãleo por semente (213mg), em comparaÃÃo com o tratamento de polinizaÃÃo aberta, e por geitonogamia (250mg). AlÃm disto, foi possÃvel constatar que hà maior produÃÃo de Ãleo por geitonogamia e nÃo por xenogamia (237mg). Conclui-se que a Apis mellifera à um polinizador eficiente da cultura. Sua introduÃÃo, com quatro colÃnias por hectare, maximiza a produÃÃo de Ãleo e produz os melhores resultados para todos os parÃmetros avaliados. AlÃm disso, apenas uma visita da abelha melÃfera Ãs flores do pinhÃo manso à suficiente para prevenir dÃficit de polinizaÃÃo.

Spiders are naturally occurring predators of insects in agroecosystems. The use of broad spectrum pesticides in agriculture is likely to disrupted spider communities and have a negative impact on their role as biocontrollers. The overall abundance, the species richness, diversity and guild structure of spiders in tropical mango orchards in central Queensland were investigated in this study. Experiments were performed to assess the potential of spiders as natural predators to pest insects in mango orchards. The effects of pesticides on the spider communities were assessed to establish the extent to which the communities were disrupted and the extent of recovery from this type of disturbance. The short term (acute) and long term (chronic) effects were investigated. The spiders in unsprayed mango orchards were relatively high in abundance, species richness and species diversity. Spiders were present in significant numbers at all sampling times,both day and at night, and during all seasons. The results suggest that spiders do not capture large numbers of prey. However spider exhibit a diversity of capturing techniques so that they capture a variety of insects. The most common guild was the orb-weavers. The spider abundance and diversity four days after spraying with methidathion suggested that recovery of spider after disruption such as the use of pesticides' occurs quickly. Presumably this recovery occurs due to spiders moving into the orchard from surrounding bush land. The long term use of pesticide does appear to disrupt the community and as evidenced by decreases in the abundance, species richness and diversity of spiders. While it is unlikely that pesticide usage will be eliminated in most commercial mango orchards, this study demonstrates that spiders are potentially important biocontrollers and that they are adversely affected by pesticide use. In the longer term, it will be desirable to develop IPM strategies to minimise pesticide use and maximise the role of spiders as biocontrollers. Such strategies will depend on studies such as this one and extensions of it.

Precision management of the mango crop can be aided by quantitative measures of indices relevant to fruit maturation and measurement of the fruit load per tree. Such measures address fruit quantity and quality, respectively. Mango fruit DM at harvest is an index of ripened fruit eating quality of ripened fruit, and DM is also useful in assessment of the stage of maturation of the fruit. This thesis considered the robustness of NIR spectroscopy based DM models for use with fruit from different harvest events and of five cultivars (Calypso™, Honey Gold, Keitt, Kensington Pride and R2E2), from two distinct growing regions (Northern Territory and Queensland, over two seasons). Individual cultivar calibrations achieved cross validation statistics of Rcv2 = 0.85-0.93; RMSECV = 0.61-0.96% DM, while a combined cultivar (global) model had satisfactory statistics of Rcv2 = 0.84; RMSECV = 0.99% DM.

The management of any supply chain rests on knowledge of stock inventory. For crops, this need involves forecast of harvest timing and volume. This thesis focusses on the use of machine vision to improve forecast of harvest volume and Near Infrared Spectroscopy (NIRS) in context of harvest timing for the Australian mango industry, with attention to issues involved in scaling up machine vision detection of fruit in images to estimation of fruit load of orchards and the issue of calibration robustness for NIRS models. A machine vision system was utilized to estimate fruit load up to six weeks before harvest on fifty four orchards over two seasons. High repeatability was achieved in night-time imaging using a multi-view machine vision method. Yield estimations were made of multiple orchards via: (i) human count of fruit load on ~5% of trees (FARM), (ii) human count of 18 trees randomly selected within three NDVI stratifications (CAL), (iii) multi-view counts (MV-Raw) and (iv) multi-view corrected for occluded fruit using manual counts of CAL trees (MV-CAL). Across the nine orchards for which results for all methods were available, the FARM, CAL, MV-Raw and MV-CAL methods achieved an average percentage error on packhouse counts of 26, 13, 11 and 17 %, with SD of 4, 11, 8 and 11 %, respectively, in the 2019-20 season. Greater error in load estimation occurred in the 2020-21 season due to the time-spread of flowering. However, canopy architecture impacted performance, with reasonable estimates achieved on hedge, single leader, and conventional systems (3.4, 5.0, and 8.2 average percentage error, respectively) while fruit load of trellised orchards was over-estimated. The potential for agronomic interventions to shift fruit load from top of canopy to side of canopy was therefore explored, with the aim of improving fruit visibility from the inter-row and improving harvest efficiency. Upper canopy panicles were removed by pruning in two seasons. In both seasons the proportion of fruit detected by machine vision was significantly increased by the pruning treatment compared to the control (1.51 c.f. 1.19 and 1.56 c.f. 0.88; for 2019/20 and 2020/21 seasons respectively). Fruit were also shifted to the outer canopy as assessed in terms of camera to fruit distances measured using a Time of Flight camera in the 2020/21 season (2238 c.f. 2411 µ distance fruit to camera [mm] for treatment and control row respectively). In another approach, upper canopy vegetative growth was removed at intervals before the floral induction period, preventing formation of upper canopy floral panicles but without decrease in fruit load per tree (control = 61.5; treatments = 59.5 - 74.2 fruit/tree). Short wave near infrared spectroscopy based on Partial Least Squares Regression (PLSR) models has found use in non-invasive assessment of Dry Matter Content (DMC, % fresh weight) of mango fruit. A data set of 4,675 samples acquired across multiple seasons, cultivars and growing regions was assembled for use in training, with harvest populations used as cross-validation groups and data of the fourth season reserved as an independent test set. The fruit physiological stage had the greatest impact on PLSR model performance, compared to cultivar, year or growing region, as estimated using a ‘variable importance metric’. The use of a global ANN model (0.89 %) gave prediction results comparable to specific (cultivar or physiological stage) PLSR models (RMSEP on DMC decreased from 1.01 to 0.88 %). A companion study compared ANN, Gaussian Process Regression (GPR), Local Optimized by Variance Regression (LOVR), Local Partial Least use Squares Regression (LPLS), Local PLS Scores (LPLS-S) and Memory Based Learner (MBL) and two commercially available cloud-based chemometric packages. All of these models gave a better result than a global PLSR model. The best result (lowest RMSEP) was achieved with an ensemble of ANN, GPR and LPLS-S, with the best individual model result achieved by LOVR, with RMSEP of 0.839 % and 0.881 %, respectively, compared to the global PLSR result of 1.014 %. The data set was made public and has been used in multiple publications by other authors, with introduction of deep learning (convolution neural network) models, although direct comparison to the models of this thesis was not possible due to differences in outlier removal. The ANN model was recommended as satisfactory in all categories (model size, prediction speed, model build speed, and prediction statistics) and insensitive to tuning. This ANN model was adopted across handheld spectrometers used in the Australian mango industry in the 2020/21 season.

A machine vision based system is proposed to replace the current in-orchard manual estimates of mango fruit yield, to inform harvest resourcing and marketing. The state-of-the-art in fruit detection was reviewed, highlighting the recent move from traditional image segmentation methods to convolution neural network (CNN) based deep learning methods. An experimental comparison of several deep learning based object detection frameworks (single shot detectors versus two-staged detectors) and several standard CNN architectures was undertaken for detection of mango panicles and fruit in tree images. The machine vision system used images of individual trees captured during night time from a moving platform mounted with a Global Navigation Satellite System (GNSS) receiver and a LED panel floodlight. YOLO, a single shot object detection framework, was re-designed and named as MangoYOLO. MangoYOLO outperformed existing state-of-the-art deep learning object detection frameworks in terms of fruit detection time and accuracy and was robust in use across different cultivars and cameras. MangoYOLO achieved F1 score of 0.968 and average precision of 0.983 and required just 70 ms per image (2048 × 2048 pixel) and 4417 MB memory. The annotated image dataset was made publicly available. Approaches were trialled to relate the fruit counts from tree images to the actual harvest count at an individual tree level. Machine vision based estimates of fruit load ranged between -11% to +14% of packhouse fruit counts. However, estimation of fruit yield (t/ha) requires estimation of fruit size as well as fruit number. A fruit sizing app for smart phones was developed as an affordable in-field solution. The solution was based on segmentation of the fruit in image using colour features and estimation of the camera to fruit perimeter distance based on use of fruit allometrics. For mango fruit, RMSEs of 5.3 and 3.7 mm were achieved on length and width measurements under controlled lighting, and RMSEs of 5.5 and 4.6 mm were obtained in-field under ambient lighting. Further, estimation of harvest timing can be informed by assessment of the spread of flowering. Deep learning object detection methods were deployed for assessment of the number and development stage of mango panicles, on tree. Methods to deal with different orientations of flower panicles in tree images were implemented. An R2 >0.8 was achieved between machine vision count of panicles on images and in-field human count per tree. Similarly, mean average precision of 69.1% was achieved for classification of panicle stages. These machine vision systems form a foundation for estimation of crop load and harvest timing, and for automated harvesting.

This thesis is an investigation into the cross-cultural appropriation of manga and anime by fans in Australia. I investigate the way in which fans embark on ‘identity’ projects through manga and anime to construct a space where issues of gender politics, identity and culture are explored. I argue that a key reason why many Western fans and scholars perceive manga and anime as ‘different’ is its ‘Japaneseness’. The two key problems addressed throughout the thesis are : how can we analyse the significance of the Japanese origins and context of manga and anime, and would the ‘identity projects’ that fans construct be possible without an appreciation of manga and anime’s 'Japaneseness?.' These questions are explored in terms of a number of key forms within manga and anime including cyberpunk, bishonen(beautiful boys), otaku(fans) and anime forms that have had their ‘Japaneseness’ softened. I discuss the way in which these manga and anime forms offer different spaces for fans, scholars and cultural industries to contest, rework and reiterate the cultural value of manga and anime.
Doctor of Philosophy (PhD)

Prediction of the optimal time of harvest and the harvest fruit load is required by mango orchard managers. Four technologies were assessed: (i) field temperature sensors and heat units; (ii) non-destructive assessment of fruit flesh colour; (iii) machine vision for flowering and fruit load assessment; and (iv) fruit size estimation.

碩士
國立中正大學
傳播學系電訊傳播研究所
105
Nowadays, in addition to manga-based anime, the live-action version is becoming a trend. Manga, being remediated to different media modality, provides rich choices for the audience. This study aims to explore cross-media participation behavior and interpretation, and the effect of entry point by focusing on “manga audiences.” Taking into account the media modality and the entry point, the motivations of manga audiences consuming manga or adaptations are re-experiencing what they have known and exploring more details of the story world. The findings also indicate that the perception of intermediality/intertextuality affects audiences’ interpretation of manga and adaptations, and their pleasures. Moreover, through the implementation of the actual viewing behavior, this study finds out that the first viewing medium (the entry point) will become the reference point for other texts. The finding also subverts the idea of manga as the main, adaptation as the supplement.

碩士
國立臺灣大學
外國語文學研究所
105
This thesis examines the image of Victorian female maids and seamstresses represented in Japanese neo-Victorian manga and fiction, with special references to shokunin (しょくにん; 職人; professional craftsmen) in Japanese culture and neo-Victorianism. The core texts studied are Kaoru Mori (森薰)’s Emma (2006) and Yuko Aoki (青木佑子)’s Victorian Rose Tailor (2010). The characters of neo-Victorian needlewomen and housemaids, I argue, deserve scholarly attention and can be treated in ways that differ from those kawaii maids in mainstream Japanese ACGN (Anime, Comics, Games, Novels) or simply beautiful passive housewives and “interactive personal robots” from the perspective of certain Western post-feminists. Instead, I see them as hybridized cultural icons that retain the conventional characteristics of Victorian working women, while simultaneously containing the essence of the Japanese “shokunin spirit.” Contesting previous scholars’ readings, the thesis demonstrates that the hybridized representations of Victorian female workers in Emma and Victorian Rose Tailor exemplify the ways in which Japanese neo-Victorian works ingeniously embed the Japanese shokunin spirit into the Western settings of Victorian England and further reflect the shared mentality of class fluidity in different contexts. In addition, the seemingly “Cinderella-esque” romance that these neo-Victorian shokunin encounter also revisits the cross-class Victorian romance between lower working-class females and middle or upper-class employers, and disrupts the dichotomies between “Angel in the House” and “New Woman” in nineteenth-century England.