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1

Kim, M.-H. Y., S. A. Thibeault, J. W. Wilson, et al. "Development and Testing of in situ Materials for Human Exploration of Mars." High Performance Polymers 12, no. 1 (2000): 13–26. http://dx.doi.org/10.1088/0954-0083/12/1/302.

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Interplanetary space radiation poses a serious health hazard in long-term manned space missions. Natural Martian surface materials are evaluated for their potential use as radiation shields for manned Mars missions. The modified radiation fluences behind various kinds of Martian rocks and regolith are determined by solving the Boltzmann equation using NASA Langley’s HZETRN code along with the 1977 Solar Minimum galactic cosmic ray environmental model. To make structural shielding composite materials from constituents of the Martian atmosphere and from Martian regolith for Martian surface habit
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2

Oze, Christopher, Joshua Beisel, Edward Dabsys, et al. "Perchlorate and Agriculture on Mars." Soil Systems 5, no. 3 (2021): 37. http://dx.doi.org/10.3390/soilsystems5030037.

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Perchlorate (ClO4−) is globally enriched in Martian regolith at levels commonly toxic to plants. Consequently, perchlorate in Martian regolith presents an obstacle to developing agriculture on Mars. Here, we assess the effect of perchlorate at different concentrations on plant growth and germination, as well as metal release in a simulated Gusev Crater regolith and generic potting soil. The presence of perchlorate was uniformly detrimental to plant growth regardless of growing medium. Plants in potting soil were able to germinate in 1 wt.% perchlorate; however, these plants showed restricted g
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Kaksonen, Anna H., Xiao Deng, Christina Morris, Himel Nahreen Khaleque, Luis Zea, and Yosephine Gumulya. "Potential of Acidithiobacillus ferrooxidans to Grow on and Bioleach Metals from Mars and Lunar Regolith Simulants under Simulated Microgravity Conditions." Microorganisms 9, no. 12 (2021): 2416. http://dx.doi.org/10.3390/microorganisms9122416.

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The biomining microbes which extract metals from ores that have been applied in mining processes worldwide hold potential for harnessing space resources. Their cell growth and ability to extract metals from extraterrestrial minerals under microgravity environments, however, remains largely unknown. The present study used the model biomining bacterium Acidithiobacillus ferrooxidans to extract metals from lunar and Martian regolith simulants cultivated in a rotating clinostat with matched controls grown under the influence of terrestrial gravity. Analyses included assessments of final cell count
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4

Harris, Franklin, John Dobbs, David Atkins, James A. Ippolito, and Jane E. Stewart. "Soil fertility interactions with Sinorhizobium-legume symbiosis in a simulated Martian regolith; effects on nitrogen content and plant health." PLOS ONE 16, no. 9 (2021): e0257053. http://dx.doi.org/10.1371/journal.pone.0257053.

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Due to increasing population growth and declining arable land on Earth, astroagriculture will be vital to terraform Martian regolith for settlement. Nodulating plants and their N-fixing symbionts may play a role in increasing Martian soil fertility. On Earth, clover (Melilotus officinalis) forms a symbiotic relationship with the N-fixing bacteria Sinorhizobium meliloti; clover has been previously grown in simulated regolith yet without bacterial inoculation. In this study, we inoculated clover with S. meliloti grown in potting soil and regolith to test the hypothesis that plants grown in regol
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Shumway, Andrew O., David C. Catling, and Jonathan D. Toner. "Regolith Inhibits Salt and Ice Crystallization in Mg(ClO4)2 Brine, Implying More Persistent and Potentially Habitable Brines on Mars." Planetary Science Journal 4, no. 8 (2023): 143. http://dx.doi.org/10.3847/psj/ace891.

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Abstract On Mars, liquid water may form in regolith when perchlorate salts absorb water vapor and dissolve into brine, or when ice-salt mixtures reach their melting temperature and thaw. Brines created in this way can chemically react with minerals, alter the mechanical properties of regolith, mobilize salts in the soil, and potentially create habitable environments. Although Martian brines would exist in contact with regolith, few studies have investigated how regolith alters the formation and stability of brines at Mars-relevant conditions. To fill this gap, we studied magnesium perchlorate
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6

Sakon, John J., and Robert L. Burnap. "An analysis of potential photosynthetic life on Mars." International Journal of Astrobiology 5, no. 2 (2006): 171–80. http://dx.doi.org/10.1017/s1473550406003144.

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This project researched the possibility of photosynthetic life on Mars. Cyanobacteria were used as potential analogs and were subjected to various Martian-simulated conditions. Synechocystis sp. PCC 6803 was exposed to low pressure, ultraviolet radiation and Martian-simulated atmospheric composition, and proved resistant to the combination of these stresses. However, this organism could neither grow within Martian Regolith Simulant, owing to the lack of soluble nitrogen, nor could it grow in cold temperatures. As a result, later research focused on psychrotolerant cyanobacteria capable of util
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7

Simonsen, L. C., J. E. Nealy, L. W. Townsend, and J. W. Wilson. "Martian regolith as space radiation shielding." Journal of Spacecraft and Rockets 28, no. 1 (1991): 7–8. http://dx.doi.org/10.2514/3.26201.

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8

Seiferlin, Karsten, Pascale Ehrenfreund, James Garry, et al. "Simulating Martian regolith in the laboratory." Planetary and Space Science 56, no. 15 (2008): 2009–25. http://dx.doi.org/10.1016/j.pss.2008.09.017.

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9

Jackiewicz, E., M. Lukasiak, M. Kopcewicz, K. Szpila, and N. Bakun-Czubarow. "Mössbauer study of Martian regolith analogues." Hyperfine Interactions 70, no. 1-4 (1992): 993–96. http://dx.doi.org/10.1007/bf02397495.

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10

Rahim, Abdur, Umair Majeed, Muhammad Irfan Zubair, and Muhammad Shahzad. "WNMS: A New Basaltic Simulant of Mars Regolith." Sustainability 15, no. 18 (2023): 13372. http://dx.doi.org/10.3390/su151813372.

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The use of planetary regolith can be explored via the utilization of simulants. The existing Martian simulants have differences due to varying source materials and design parameters. Additional simulants are needed because the few available simulants do not replicate the compositional diversity of Martian regolith. This study discusses the development of a low-cost construction simulant of Mars. The area of Winder Nai in Pakistan was selected for field sampling of basalt because of local availability and easy access. The dust was produced from rock samples through mechanical crushing and grind
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11

Kasiviswanathan, Pooja, Elizabeth D. Swanner, Larry J. Halverson, and Paramasivan Vijayapalani. "Farming on Mars: Treatment of basaltic regolith soil and briny water simulants sustains plant growth." PLOS ONE 17, no. 8 (2022): e0272209. http://dx.doi.org/10.1371/journal.pone.0272209.

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A fundamental challenge in human missions to Mars is producing consumable foods efficiently with the in situ resources such as soil, water, nutrients and solar radiation available on Mars. The low nutrient content of martian soil and high salinity of water render them unfit for direct use for propagating food crops on Mars. It is therefore essential to develop strategies to enhance nutrient content in Mars soil and to desalinate briny water for long-term missions on Mars. We report simple and efficient strategies for treating basaltic regolith simulant soil and briny water simulant for suitabl
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12

Fackrell, Laura E., Paul A. Schroeder, Aaron Thompson, Karen Stockstill-Cahill, and Charles A. Hibbitts. "Development of martian regolith and bedrock simulants: Potential and limitations of martian regolith as an in-situ resource." Icarus 354 (January 2021): 114055. http://dx.doi.org/10.1016/j.icarus.2020.114055.

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13

Hedayati, Reza, and Victoria Stulova. "3D Printing of Habitats on Mars: Effects of Low Temperature and Pressure." Materials 16, no. 14 (2023): 5175. http://dx.doi.org/10.3390/ma16145175.

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Due to payload weight limitations and human vulnerability to harsh space conditions, it is preferable that the potential landing location for humans has an already constructed habitat preferably made from in situ materials. Therefore, the prospect of utilizing a readily available Martian material, such as regolith, in an easily programmable manufacturing method, such as 3D printing, is very lucrative. The goal of this research is to explore a mixture containing Martian regolith for the purposes of 3D printing in unfavorable conditions. A binder consisting of water and sodium silicate is used.
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14

Wamelink, G. W. W., J. Y. Frissel, W. H. J. Krijnen, and M. R. Verwoert. "Crop growth and viability of seeds on Mars and Moon soil simulants." Open Agriculture 4, no. 1 (2019): 509–16. http://dx.doi.org/10.1515/opag-2019-0051.

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AbstractIf humans are going to establish a base on the Moon or on Mars they will have to grow their own crops. An option is to use Lunar and Martian regolith. These regoliths are not available for plant growth experiments, therefore NASA has developed regolith simulants. The major goal of this project was to cultivate and harvest crops on these Mars and Moon simulants. The simulants were mixed with organic matter to mimic the addition of residues from earlier harvests. Ten different crops, garden cress, rocket, tomato, radish, rye, quinoa, spinach, chives, pea and leek were sown in random line
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15

Martikainen, Julia, Olga Muñoz, Teresa Jardiel, et al. "Optical Constants of Martian Dust Analogs at UV–Visible–Near-infrared Wavelengths." Astrophysical Journal Supplement Series 268, no. 2 (2023): 47. http://dx.doi.org/10.3847/1538-4365/acf0be.

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Abstract We present an advanced light-scattering model to retrieve the optical constants of three Martian dust analogs: Johnson Space Center regolith simulant, Enhanced Mojave Mars Simulant, and Mars Global Simulant. The samples are prepared to have narrow particle-size distributions within the geometric-optics domain. We carry out laboratory measurements to obtain the particle-size distributions, shapes, and diffuse reflectance spectra of the Martian analogs deposited on a surface. Our model framework includes a ray-optics code to compute scattering properties for individual particles, and a
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Pacelli, Claudia, Alessia Cassaro, Lorenzo Aureli, Ralf Moeller, Akira Fujimori, and Silvano Onofri. "The Responses of the Black Fungus Cryomyces Antarcticus to High Doses of Accelerated Helium Ions Radiation within Martian Regolith Simulants and Their Relevance for Mars." Life 10, no. 8 (2020): 130. http://dx.doi.org/10.3390/life10080130.

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One of the primary current astrobiological goals is to understand the limits of microbial resistance to extraterrestrial conditions. Much attention is paid to ionizing radiation, since it can prevent the preservation and spread of life outside the Earth. The aim of this research was to study the impact of accelerated He ions (150 MeV/n, up to 1 kGy) as a component of the galactic cosmic rays on the black fungus C. antarcticus when mixed with Antarctic sandstones—the substratum of its natural habitat—and two Martian regolith simulants, which mimics two different evolutionary stages of Mars. The
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17

Fabian, A., C. Krauss, A. Sickafoose, M. Horanyi, and S. Robertson. "Measurements of electrical discharges in Martian regolith simulant." IEEE Transactions on Plasma Science 29, no. 2 (2001): 288–91. http://dx.doi.org/10.1109/27.923710.

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18

Ramkissoon, Nisha K., Victoria K. Pearson, Susanne P. Schwenzer, et al. "New simulants for martian regolith: Controlling iron variability." Planetary and Space Science 179 (December 2019): 104722. http://dx.doi.org/10.1016/j.pss.2019.104722.

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19

Eichler, A., N. Hadland, D. Pickett, et al. "Challenging the agricultural viability of martian regolith simulants." Icarus 354 (January 2021): 114022. http://dx.doi.org/10.1016/j.icarus.2020.114022.

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20

Vakkada Ramachandran, Abhilash, María-Paz Zorzano, and Javier Martín-Torres. "Experimental Investigation of the Atmosphere-Regolith Water Cycle on Present-Day Mars." Sensors 21, no. 21 (2021): 7421. http://dx.doi.org/10.3390/s21217421.

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The water content of the upper layers of the surface of Mars is not yet quantified. Laboratory simulations are the only feasible way to investigate this in a controlled way on Earth, and then compare it with remote and in situ observations of spacecrafts on Mars. Describing the processes that may induce changes in the water content of the surface is critical to determine the present-day habitability of the Martian surface, to understand the atmospheric water cycle, and to estimate the efficiency of future water extraction procedures from the regolith for In Situ Resource Utilization (ISRU). Th
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21

Raúl G, Cuero. "Martian soil as a potential source of nanoparticles: Study using martian regolith simulant." Frontiers in Nanoscience and Nanotechnology 2, no. 2 (2016): 91–99. http://dx.doi.org/10.15761/fnn.1000115.

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22

Boxe, C. S., K. P. Hand, K. H. Nealson, Y. L. Yung, A. S. Yen, and A. Saiz-Lopez. "Adsorbed water and thin liquid films on Mars." International Journal of Astrobiology 11, no. 3 (2012): 169–75. http://dx.doi.org/10.1017/s1473550412000080.

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AbstractAt present, bulk liquid water on the surface and near-subsurface of Mars does not exist due to the scarcity of condensed- and gas-phase water, pressure and temperature constraints. Given that the nuclei of soil and ice, that is, the soil solid and ice lattice, respectively, are coated with adsorbed and/or thin liquid films of water well below 273 K and the availability of water limits biological activity, we quantify lower and upper limits for the thickness of such adsorbed/water films on the surface of the Martian regolith and for subsurface ice. These limits were calculated based on
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23

Menlyadiev, Marlen, Bryana L. Henderson, Fang Zhong, Ying Lin, and Isik Kanik. "Extraction of amino acids using supercritical carbon dioxide forin situastrobiological applications." International Journal of Astrobiology 18, no. 2 (2018): 102–11. http://dx.doi.org/10.1017/s147355041800006x.

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AbstractThe detection of organic molecules that are indicative of past or present biological activity within the Solar System bodies and beyond is a key research area in astrobiology. Mars is of particular interest in this regard because of evidence of a (perhaps transient) warm and wet climate in its past. To date, space missions to Mars have primarily used pyrolysis technique to extract organic compounds from the Martian regolith, but it has not enabled a clear detection of unaltered native Martian organics. The elevated temperatures required for pyrolysis extraction can cause native Martian
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Barkatt, Aaron, and Masataka Okutsu. "Obtaining elemental sulfur for Martian sulfur concrete." Journal of Chemical Research 46, no. 2 (2022): 174751982210807. http://dx.doi.org/10.1177/17475198221080729.

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A potential candidate material for the construction of Mars habitats is concrete made from the Martian regolith and sulfur extracted from the regolith itself. Sulfur concrete, which has excellent mechanical properties, can be prepared at a low temperature (<150 °) and without water (unlike Portland-cement concrete). The surface of Mars has a much higher concentration of sulfur than those of the Earth, the Moon, or the asteroids. Sulfur on Mars, however, exists not as elemental sulfur—which is needed in concrete production—but as sulfates (usually hydrated) and sulfides. This paper surveys t
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Noe Dobrea, E. Z., J. F. Bell, M. J. Wolff, and K. D. Gordon. "H2O- and OH-bearing minerals in the martian regolith:." Icarus 166, no. 1 (2003): 1–20. http://dx.doi.org/10.1016/s0019-1035(03)00208-2.

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26

Gori, Fabio, and Sandra Corasaniti. "Detection of a dry–frozen boundary inside Martian regolith." Planetary and Space Science 56, no. 8 (2008): 1093–102. http://dx.doi.org/10.1016/j.pss.2008.02.003.

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27

Audouard, Joachim, François Poulet, Mathieu Vincendon, et al. "Water in the Martian regolith from OMEGA/Mars Express." Journal of Geophysical Research: Planets 119, no. 8 (2014): 1969–89. http://dx.doi.org/10.1002/2014je004649.

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28

Maurel, Alexis, Ana Cristina Martinez, Pedro Cortes, et al. "3D Printing of Batteries from Lunar and Martian Regolith." ECS Meeting Abstracts MA2023-01, no. 56 (2023): 2724. http://dx.doi.org/10.1149/ma2023-01562724mtgabs.

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To maximize the sustainability of future space missions, the utilization of local resources available on the Moon or Mars, also known as in-situ resource utilization (ISRU), is crucial to develop infrastructures such as habitation modules, power generation, and energy storage facilities.1–3 This work presents a perspective aiming to introduce the future of batteries manufacturing on the lunar and martian environment from ISRU materials. Based on the composition of the lunar and martian soil,4–7 the choice of the battery technology and materials for the different battery components (electrodes,
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Rao, M. "Neutron Capture Isotopes in the Martian Regolith and Implications for Martian Atmospheric Noble Gases." Icarus 156, no. 2 (2002): 352–72. http://dx.doi.org/10.1006/icar.2001.6809.

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30

Yin, Kexin, Jiangxin Liu, Jiaxing Lin, Andreea-Roxana Vasilescu, Khaoula Othmani, and Eugenia Di Filippo. "Interface Direct Shear Tests on JEZ-1 Mars Regolith Simulant." Applied Sciences 11, no. 15 (2021): 7052. http://dx.doi.org/10.3390/app11157052.

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The mechanical behaviors of Martian regolith-structure interfaces are of great significance for the design of rover, development of excavation tools, and construction of infrastructure in Mars exploration. This paper presents an experimental investigation on the properties of a Martian regolith simulant (JEZ-1) through one-dimensional oedometer test, direct shear test, and interface direct shear tests between JEZ-1 and steel plates with different roughness. Oedometer result reveals that the compression and swelling indexes of the JEZ-1 are quite low, thus it is a less compressible and lower sw
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31

Nénon, Q., A. R. Poppe, A. Rahmati, and J. P. McFadden. "Implantation of Martian atmospheric ions within the regolith of Phobos." Nature Geoscience 14, no. 2 (2021): 61–66. http://dx.doi.org/10.1038/s41561-020-00682-0.

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32

Jach, K., J. Leliwa-Kopystyński, A. Morka, et al. "Modifications of Martian ice-saturated regolith due to meteoroid impact." Advances in Space Research 23, no. 11 (1999): 1933–37. http://dx.doi.org/10.1016/s0273-1177(99)00275-6.

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33

Garry, James R. C., Inge Loes ten Kate, Zita Martins, Per Nørnberg, and Pascale Ehrenfreund. "Analysis and survival of amino acids in Martian regolith analogs." Meteoritics & Planetary Science 41, no. 3 (2006): 391–405. http://dx.doi.org/10.1111/j.1945-5100.2006.tb00470.x.

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34

Pavlov, A. K., V. N. Shelegedin, M. A. Vdovina, and A. A. Pavlov. "Growth of microorganisms in Martian-like shallow subsurface conditions: laboratory modelling." International Journal of Astrobiology 9, no. 1 (2009): 51–58. http://dx.doi.org/10.1017/s1473550409990371.

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AbstractLow atmospheric pressures on Mars and the lack of substantial amounts of liquid water were suggested to be among the major limiting factors for the potential Martian biosphere. However, large amounts of ice were detected in the relatively shallow subsurface layers of Mars by the Odyssey Mission and when ice sublimates the water vapour can diffuse through the porous surface layer of the soil. Here we studied the possibility for the active growth of microorganisms in such a vapour diffusion layer. Our results showed the possibility of metabolism and the reproduction of non-extremophile t
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35

Patel, M. R., A. Bérces, C. Kolb, et al. "Seasonal and diurnal variations in Martian surface ultraviolet irradiation: biological and chemical implications for the Martian regolith." International Journal of Astrobiology 2, no. 1 (2003): 21–34. http://dx.doi.org/10.1017/s1473550402001180.

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The issue of the variation of the surface ultraviolet (UV) environment on Mars was investigated with particular emphasis being placed on the interpretation of data in a biological context. A UV model has been developed to yield the surface UV irradiance at any time and place over the Martian year. Seasonal and diurnal variations were calculated and dose rates evaluated. Biological interpretation of UV doses is performed through the calculation of DNA damage effects upon phage T7 and Uracil, used as examples for biological dosimeters. A solar UV ‘hotspot’ was revealed towards perihelion in the
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36

Gleaton, Jason, Zhengshou Lai, Rui Xiao, Ke Zhang, Qiushi Chen, and Yi Zheng. "Optimization of mechanical strength of biocemented Martian regolith simulant soil columns." Construction and Building Materials 315 (January 2022): 125741. http://dx.doi.org/10.1016/j.conbuildmat.2021.125741.

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37

Dikshit, Rashmi, Nitin Gupta, Arjun Dey, Koushik Viswanathan, and Aloke Kumar. "Microbial induced calcite precipitation can consolidate martian and lunar regolith simulants." PLOS ONE 17, no. 4 (2022): e0266415. http://dx.doi.org/10.1371/journal.pone.0266415.

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We demonstrate that Microbial Induced Calcite Precipitation (MICP) can be utilized for creation of consolidates of Martian Simulant Soil (MSS) and Lunar Simulant Soil (LSS) in the form of a ‘brick’. A urease producer bacterium, Sporosarcina pasteurii, was used to induce the MICP process for the both simulant soils. An admixture of guar gum as an organic polymer and NiCl2, as bio- catalyst to enhance urease activity, was introduced to increase the compressive strength of the biologically grown bricks. A casting method was utilized for a slurry consisting of the appropriate simulant soil and mic
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38

Zent, Aaron P. "On the thickness of the oxidized layer of the Martian regolith." Journal of Geophysical Research: Planets 103, E13 (1998): 31491–98. http://dx.doi.org/10.1029/98je01895.

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39

Wittmann, Axel, Randy L. Korotev, Bradley L. Jolliff, et al. "Petrography and composition of Martian regolith breccia meteorite Northwest Africa 7475." Meteoritics & Planetary Science 50, no. 2 (2015): 326–52. http://dx.doi.org/10.1111/maps.12425.

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40

Zent, Aaron P., Fraser P. Fanale, and Susan E. Postawko. "Carbon dioxide: Adsorption on palagonite and partitioning in the Martian regolith." Icarus 71, no. 2 (1987): 241–49. http://dx.doi.org/10.1016/0019-1035(87)90149-7.

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41

Marshall, Jason P., Troy L. Hudson, and José E. Andrade. "Experimental Investigation of InSight HP3 Mole Interaction with Martian Regolith Simulant." Space Science Reviews 211, no. 1-4 (2017): 239–58. http://dx.doi.org/10.1007/s11214-016-0329-1.

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42

Gunderson, Kurt, Benjamin Lüthi, Patrick Russell, and Nicolas Thomas. "Visible/NIR photometric signatures of liquid water in Martian regolith simulant." Planetary and Space Science 55, no. 10 (2007): 1272–82. http://dx.doi.org/10.1016/j.pss.2007.03.004.

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43

Shiwei, Ng, Stylianos Dritsas, and Javier G. Fernandez. "Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing." PLOS ONE 15, no. 9 (2020): e0238606. http://dx.doi.org/10.1371/journal.pone.0238606.

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44

Fanale, Fraser P., and James R. Salvail. "Quasi-periodic Atmosphere-Regolith-Cap CO2 Redistribution in the Martian Past." Icarus 111, no. 2 (1994): 305–16. http://dx.doi.org/10.1006/icar.1994.1147.

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45

Böttger, H. M., S. R. Lewis, P. L. Read, and F. Forget. "The effects of the martian regolith on GCM water cycle simulations." Icarus 177, no. 1 (2005): 174–89. http://dx.doi.org/10.1016/j.icarus.2005.02.024.

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46

Dotson, B., D. Sanchez Valencia, C. Millwater, et al. "Cohesion and shear strength of compacted lunar and Martian regolith simulants." Icarus 411 (March 2024): 115943. http://dx.doi.org/10.1016/j.icarus.2024.115943.

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47

Pajares, Arturo, Pablo Guardia, Vladimir Galvita, Melchiorre Conti, Jasper Lefevere, and Bart Michielsen. "CO2 conversion over Martian and Lunar regolith simulants for extraterrestrial applications." Journal of CO2 Utilization 81 (March 2024): 102729. http://dx.doi.org/10.1016/j.jcou.2024.102729.

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48

Corrias, Gianluca, Roberta Licheri, Roberto Orrù, and Giacomo Cao. "Self-Propagating High-Temperature Synthesis Reactions for ISRU and ISFR Applications." Eurasian Chemico-Technological Journal 13, no. 3-4 (2010): 137. http://dx.doi.org/10.18321/ectj77.

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<p>In the framework of ISRU (In-Situ Resource Utilization) and ISFR (In-Situ Fabrication and Repair) applications, a novel recently patented process based on the occurrence of Self-propagating High temperature Synthesis (SHS) reactions potentially exploitable for the in-situ fabrication of construction materials in Lunar and Martian environments is described in this work. Specifically, the SHS process involves thermite reactions type between Lunar or Martian regolith simulants and aluminum as reducing agent. To overcome the fact that the original content of ilmenite (FeTiO<sub>3&lt
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49

Oliver, James A. W., Matthew Kelbrick, Nisha K. Ramkissoon, et al. "Sulfur Cycling as a Viable Metabolism under Simulated Noachian/Hesperian Chemistries." Life 12, no. 4 (2022): 523. http://dx.doi.org/10.3390/life12040523.

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Water present on the surface of early Mars (>3.0 Ga) may have been habitable. Characterising analogue environments and investigating the aspects of their microbiome best suited for growth under simulated martian chemical conditions is key to understanding potential habitability. Experiments were conducted to investigate the viability of microbes from a Mars analogue environment, Colour Peak Springs (Axel Heiberg Island, Canadian High Arctic), under simulated martian chemistries. The fluid was designed to emulate waters thought to be typical of the late Noachian, in combination with regolith
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Vezzola, Michele, Solveig Tosi, Enrico Doria, Mattia Bonazzi, Matteo Alvaro, and Alessio Sanfilippo. "Interaction between a Martian Regolith Simulant and Fungal Organic Acids in the Biomining Perspective." Journal of Fungi 9, no. 10 (2023): 976. http://dx.doi.org/10.3390/jof9100976.

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The aim of this study was to evaluate the potential of Aspergillus tubingensis in extracting metals from rocks simulating Martian regolith through biomining. The results indicated that the fungal strain produced organic acids, particularly oxalic acid, in the first five days, leading to a rapid reduction in the pH of the culture medium. This acidic medium is ideal for bioleaching, a process that employs acidolysis and complexolysis to extract metals from rocks. Additionally, the strain synthesized siderophores, molecules capable of mobilizing metals from solid matrices, as verified by the blue
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