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1

Al-Rasheed, Agharid A., Sana’a S. Ahmed, Karim A. Al-Jashamy, and Bashiru Garb. "Immunopathological Responses to the Bovine Mastitis Associated with Staphylococcus Species Infection." Iraqi Journal of Veterinary Medicine 46, no. 2 (2022): 7–11. http://dx.doi.org/10.30539/ijvm.v46i2.1398.

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Bovine mastitis is a disease that concerns animals' welfare and increases the economic production losses. Bacterial agents such as Staphylococcus species are the main causative agent of bovine mastitis. This bacterial agent expresses some inflammatory cytokines that might enhance the cell-mediated, which may promote the pathogenesis of mastitis. The objective of the current study was to investigate the bovine innate immune response circulating levels of pro-inflammatory and anti-inflammatory cytokines. A total of 10 mL of milk specimens were collected randomly from 100 clinically mastitic cows
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Ahmad, T., A. Ghafoor, M. Nadeem, M. Q. Bilal, and A. Sharif. "EFFECT OF MASTITIS ON MILK COMPOSITION OF CROSS-BRED (HOLSTEIN FRIESIAN X CHOLISTANI) AND SAHIWAL CATTLE." Pakistan Journal of Agriculture, Agricultural Engineering and Veterinary Sciences 39, no. 1 (2023): 37–44. http://dx.doi.org/10.47432/2023.39.1.7.

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The present study was designed to investigate the effect of mastitis on various components including fat percentage, protein, total solids, solid not fat, pH, specific gravity and lactose content in milk of cross-bred (Holstein Friesian × Cholistani) and Sahiwal cattle. A total of 376 milk samples (excluding blind and nonfunctional teats) from 100 cows (50 cross-bred and 50 sahiwal) were collected aseptically. Animals of different ages, parity and stages of lactation were selected under field condition. The diagnosis of subclinical mastitis was done on basis of Surf Field Mastitis Test. For ba
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3

Barber, Michele R., and T. J. Yang. "Chemotactic Activities in Nonmastitic and Mastitic Mammary Secretions: Presence of Interleukin-8 in Mastitic but Not Nonmastitic Secretions." Clinical Diagnostic Laboratory Immunology 5, no. 1 (1998): 82–86. http://dx.doi.org/10.1128/cdli.5.1.82-86.1998.

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ABSTRACT Due to its association with low-quality milk and a decrease in milk production in bovines, mastitis is a major cause of economic loss. Additionally, mastitis can be harmful to suckling newborns and can cause damage to the mammary gland. In mastitic mammary secretions there is a substantial increase in somatic cells, specifically neutrophils. In this study we examined the ability of mastitic and nonmastitic mammary secretions to cause in vitro neutrophil chemotaxis using a microchemotaxis assay. Also, the role of the inflammatory chemokine interleukin-8 (IL-8) in neutrophil recruitment
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4

Yang, Yongxin, Suizhong Cao, Xiaowei Zhao, Dongwei Huang, Huiling Zhao, and Guanglong Cheng. "Determination of changes in bovine plasma and milk proteins during naturally occurring Escherichia coli mastitis by comparative proteomic analysis." Animal Production Science 56, no. 11 (2016): 1888. http://dx.doi.org/10.1071/an14901.

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The aim of this study was to investigate changes in plasma and milk proteins in response to Escherichia coli infection in cows. Plasma and milk were collected from healthy cows, cows suffering from mild E. coli mastitis, and cows suffering from severe E. coli mastitis. Protein composition was examined by two-dimensional gel electrophoresis coupled with mass spectrometry. Plasma haptoglobin and α-1 acid glycoprotein demonstrated greater expression in mastitic cows compared with controls, but there were no difference between mildly and severely mastitic cows. Milk from mildly mastitic cows showe
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5

Kocak, O. "Influence of Mastitis on Milk Yield in Holstein Cows." Acta Veterinaria Brno 75, no. 4 (2006): 507–13. http://dx.doi.org/10.2754/avb200675040507.

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The objectives of the current study were to investigate the incidence of mastitis in a private dairy herd and the effect of the disease on the lactation milk yield (LMY), and on the daily average of the weekly milk yield (DMY). Only cows with no disease and cows with mastitis were included in the analyses. Mixed model procedures were used to analyse the DMY. Incidence of mastitis in the herd was 26.22%. The incidences of first, second and third parity groups were 19.94%, 33.74% and 40.74%, respectively. Daily milk losses because of mastitis varied between 0.76 kg/d and 4.56 kg/d. The total mil
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6

Mbindyo, Christine M., George C. Gitao, and Charles M. Mulei. "Prevalence, Etiology, and Risk Factors of Mastitis in Dairy Cattle in Embu and Kajiado Counties, Kenya." Veterinary Medicine International 2020 (August 4, 2020): 1–12. http://dx.doi.org/10.1155/2020/8831172.

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Bovine mastitis continues to be a leading cause of heavy economic losses in the dairy industry and a public health hazard globally. This cross-sectional study investigated the prevalence, etiologies of clinical and subclinical mastitis, and associated predisposing factors in Embu and Kajiado counties in Kenya. A semistructured questionnaire was administered to 154 smallholder dairy farmers to collect data on management practices, animal factors, and disease history. A total of 395 dairy cows were initially screened for subclinical mastitis using the California mastitis test (CMT), and milk sam
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7

Calderon Rangel, Alfonso, Virginia Rodríguez Rodríguez, German Arrieta Bernate, and Salim Mattar Velilla. "Prevalence of mastitis in dual purpose cattle farms in Monteria (Colombia): etiology and antibacterial susceptibility." Revista Colombiana de Ciencias Pecuarias 24, no. 1 (2011): 19–28. http://dx.doi.org/10.17533/udea.rccp.324627.

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The prevalence of bovine mastitis was evaluated in 4.260 udder quarts from 1.065 cows located in 15 cattle farms managed as dual purpose systems (beef and dairy) in Monteria (Cordoba, Colombia). The farms were chosen using a non-random probability sampling design. To isolate the microorganisms involved in mastitis, milk samples were taken from the quarts with trace reaction, as well as from sub-clinical or clinically affected quarts. A high percentage (92.21%) of isolated samples included infectious organisms. The main pathogen isolated was Staphylococcus aureus (87.56% of samples), while Stre
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8

Al- Anbagi, N. A. "Daignosis of Staphylococcus aureus mastitis in bovine in Al-Najaf province by using Polymerase chain reaction (PCR)." Al-Qadisiyah Journal of Veterinary Medicine Sciences 12, no. 2 (2013): 81. http://dx.doi.org/10.29079/vol12iss2art261.

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This study was conducted to collect 388 milk samples from cows at different villages and townships in Al-Najaf province to examine about Staphylococcus aureus mastitis .CMT was used for subclinical mastitis screening ,212(54.6%) milk samples were mastitic .The molecular method (PCR assay) was used to detected the presence (glpF) gene in classically diagnosed S.aureus, which appeared that 38(92.6%) S.aureus mastitis as 13(32.5%) clinical and 25(14.5%) subclinical mastitis .There was high significant incidence of Staphylococcus aureus mastitis in left posterior udder quarter rather than others q
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9

Trujillo Torres, Carlos Mario, Andrés Felipe Gallego, Nicolás Ramírez, and Luis Guillermo Palacio Baena. "Prevalencia de mastitis bovina en sistemas doble propósito en Montería (Colombia): etiología y susceptibilidad antibacteriana." Revista Colombiana de Ciencias Pecuarias 24, no. 1 (2011): 11–18. http://dx.doi.org/10.17533/udea.rccp.324626.

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SummaryThe prevalence of bovine mastitis was evaluated in 4.260 udder quarts from 1.065 cows located in 15 cattle farms managed as dual purpose systems (beef and dairy) in Monteria (Cordoba, Colombia). The farms were chosen using a non-random probability sampling design. To isolate the microorganisms involved in mastitis, milk samples were taken from the quarts with trace reaction, as well as from sub-clinical or clinically affected quarts. A high percentage (92.21%) of isolated samples included infectious organisms. The main pathogen isolated was Staphylococcus aureus (87.56% of samples), whi
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10

Sadat, Asmaa, Alshimaa M. M. Farag, Driss Elhanafi, et al. "Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by E. coli and S. aureus Infection." Animals 13, no. 5 (2023): 892. http://dx.doi.org/10.3390/ani13050892.

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The study aimed to investigate the mastitis’ emerging causative agents and their antimicrobial sensitivity, in addition to the hematological, biochemical indicators, oxidative biomarkers, acute phase protein (APP), and inflammatory cytokine changes in dairy farms in Gamasa, Dakahlia Governorate, Egypt. One hundred Holstein Friesian dairy cattle with clinical and subclinical mastitis were investigated and were allocated into three groups based on a thorough clinical examination. E. coli and S. aureus were found responsible for the clinical and subclinical mastitis in dairy farms, respectively.
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11

Nava-Trujillo, Hector. "Effect of clinical mastitis on reproductive targets achievement in cows." Veterinária e Zootecnia 26 (February 25, 2019): 1–10. http://dx.doi.org/10.35172/rvz.2019.v26.135.

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The aim of this study was to determine the effect of clinical mastitis occurring between calving and the first service on the percentage of cows that achieve two reproductive targets: be served before 70 days postpartum and be pregnant before 110 days postpartum (dpp). Relative frequency and odds ratio (OR) with the corresponding confidence interval (CI) were calculated. Fewer mastitic cows were served before 70 dpp than non-mastitic (24.86% vs 36.59% respectively P = 0.0137); mastitic cows had lower odds to be served than non-mastitic cows (OR: 0.57; 95% CI: 0.3679-0.8938, P = 0.0141). Fewer
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12

Ferdous, J., MS Rahman, MI Khan, MAHNA Khan, and UK Rima. "Prevalence of clinical and subclinical caprine mastitis of northern region in Bangladesh." Progressive Agriculture 29, no. 2 (2018): 127–38. http://dx.doi.org/10.3329/pa.v29i2.38296.

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Mastitis is a multifactorial and infectious disease of the udder of dairy animals. Infected animals suffer from pain and fever and causing damage in udder and changes in milk. The aim of this work was to determine the prevalence of clinical and sub-clinical caprine mastitis associated with bacterial pathogens during the period from January to June, 2017. A total of 120 lactating goats were randomly selected from different villages of Dinajpur district and 240 milk samples from each udder half were aseptically collected. The udders were examined and screened for the evidence of clinical and sub
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13

Rahmeh, Rita, Abrar Akbar, Husam Alomirah, et al. "Assessment of mastitis in camel using high-throughput sequencing." PLOS ONE 17, no. 12 (2022): e0278456. http://dx.doi.org/10.1371/journal.pone.0278456.

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Camel milk is recognized as a functional food with significant economic value. Mastitis is one of the most common and costly diseases in the dairy industry. Mastitis, which is caused by pathogens such as bacteria, viruses, fungi, and algae, has an impact on the quality and quantity of milk produced as well as animal health and welfare. There is a paucity of data on the etiological factors that cause camel mastitis. This study reports the bacterial and fungal community involved in clinical camel mastitis using Illumina amplicon sequencing. A total of 25 milk samples were analyzed, including 9 s
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14

Gürler, H., GF Yarım, M. Yarım, A. Gökçeoğlu, and F. Ümit. "The effect of subclinical mastitis on milk transforming growth factor-beta level in Anatolian water buffaloes." Journal of the Hellenic Veterinary Medical Society 76, no. 1 (2025): 8899–904. https://doi.org/10.12681/jhvms.38343.

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This study aims to evaluate the relationship between transforming growth factor beta (TGF-β) and somatic cell count (SCC) in Anatolian buffalo milk. The study material comprised 80 milk samples obtained from the udder quarter of 40 healthy and 40 subclinical mastitic nonpregnant lactating Anatolian buffaloes. The California mastitis test and somatic cell count were performed on milk samples milked from four mammary lobes of buffaloes to diagnose subclinical mastitis. The milk TGF-β level was measured by bovine-specific enzyme-linked immunosorbent assay kit. The milk TGF-β levels in subclinical
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15

Swanson, Kara, Stas Gorodetsky, Laura Good та ін. "Expression of a β-Defensin mRNA, Lingual Antimicrobial Peptide, in Bovine Mammary Epithelial Tissue Is Induced by Mastitis". Infection and Immunity 72, № 12 (2004): 7311–14. http://dx.doi.org/10.1128/iai.72.12.7311-7314.2004.

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ABSTRACT The expression of a β-defensin, the lingual antimicrobial peptide (LAP), in response to mastitis was investigated by real-time PCR of RNA from mastitic and control udder quarters. There was a positive relationship between somatic cell count in milk and LAP expression. In situ hybridization showed that LAP mRNA was expressed in epithelial cells of mastitic tissue. These results suggest that LAP plays a role in the innate immune response to mastitis.
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16

Salman, Mian Muhammad, Muhammad Nawaz, Tahir Yaqub, and Muhammad Hassan Mushtaq. "Exploring the Milk Microbiota of Healthy and Mastitic Nili Ravi Buffalo Using 16S rRNA Gene Base Metagenomic Analysis." Animals 13, no. 14 (2023): 2298. http://dx.doi.org/10.3390/ani13142298.

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The Nili Ravi, a buffalo breed from Pakistan, significantly contributes to the dairy industry. Mastitis is one of the major challenges affecting milk production in this breed. The objective of the current study was to identify the bacterial communities and diversity in healthy and mastitic milk of this breed. Milk samples (n = 14) were collected from Nili Ravi buffaloes with different udder health statuses, i.e., healthy (5), subclinical mastitis (4), and clinical mastitis (5). The DNAs were extracted, subjected to partial amplification of 16S rDNA (V3 and V4 regions), and sequenced using the
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17

Ateya, Ahmed I., Samer S. Ibrahim, and Mona M. Al-Sharif. "Single Nucleotide Polymorphisms, Gene Expression and Economic Evaluation of Parameters Associated with Mastitis Susceptibility in European Cattle Breeds." Veterinary Sciences 9, no. 6 (2022): 294. http://dx.doi.org/10.3390/vetsci9060294.

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The objective of this study was to explore single nucleotide polymorphisms (SNPs), gene expression and economic evaluation of parameters associated with mastitis susceptibility in Holstein and Brown Swiss dairy cows. Two hundred and forty Holstein and Brown Swiss dairy cows (120 cows of each breed) were used in this study. The investigated dairy cows in each breed were allocated into two equal-sized groups (60 cows each); mastitis tolerant and affected groups. PCR-DNA sequencing of SELL, ABCG2, SLC11A1, FEZL, SOD1, CAT, GPX1, and AhpC/TSA revealed nucleotide sequence variations in the form of
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18

Gazim, Zilda Cristiani, Orlando Seiko Takemura, Luiz Cláudio Monteiro da Silva, et al. "Preliminary study of homeopathic treatment of subclinical mastitis evaluated through somatic cells count (SCC) and California mastitis test (CMT)." International Journal of High Dilution Research - ISSN 1982-6206 7, no. 24 (2021): 147–51. http://dx.doi.org/10.51910/ijhdr.v7i24.292.

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The present paper presents the results of homeopathic treatment of 25 Holstein breed cows aged 3 to 8 years old diagnosed with subclinical mastitis through California Mastitis Test (CMT). Animals were divided into 3 groups according with infection level. A homeopathic complex was developed on the grounds of clinical aspects, including Phosphorus 30x, Phytolacca 30x, Silicea 30x, Sulphur 30x, Belladona 30x, Bryonia alba 30x, Pulsatilla 30x, Calendula 30x and biotherapic of Staphylococcus aureus 200x. The remedy was added to salt and was administered to cattle 100g/cow/day for 75 days. CMT were
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19

El-Diasty, Mohamed, Hager Talaat, Samar Atwa, Elzahraa Elbaz, and Mohamed Eissa. "Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows." September2019 20, no. 3 (2019): 35–39. http://dx.doi.org/10.35943/mvmj.2019.23.207.

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Objective: To study the prevalence of Coagulase-negative Staphylococci in clinical and subclinical mastitis in dairy cows. Design: Case-control study. Animals: 415 lactating Holstein cows. Procedures: A total of 896 quarter milk samples were collected aseptically from 415 dairy cows for bacteriological examination (mastitic cows, n=50; healthy cows, n=174). Identification of different bacterial isolates followed by antibiotic sensitivity test for Coagulase-negative Staphylococci (CNS) was carried out, using disc-diffusion method against 13 different antibiotics. Results: 224 out of 415 cows (5
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20

Afrilia, Tika Fitria Wulan, Riska Faradila, Ananda Rifqi Shofi, and Ahmad Drajat Arifianto. "Deteksi mastitis subklinis pada peternakan sapi perah di wilayah kanigoro, Blitar." VITEK : Bidang Kedokteran Hewan 11, no. 2 (2021): 71–73. http://dx.doi.org/10.30742/jv.v11i2.79.

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Mastitis is an inflammatory of the udder internal tissue with different causes and degrees of severity, duration and diseases caused by vaiorus causative agent. This disease is very harmfull because it caused a reduction in milk production, the additional costs of medicines causing economic losses for farmers. This tesearch was a descriptive research. Detection was done by talking milk sampel on dairy cattle and was reacted with California Mastits Test (CMT) reagents which were circukated circuary for 10-15 seconds. This result about mastitis subklinis tested using by California Mastitis Test
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21

Essa, Bothaina, Mona Al-Sharif, Mohamed Abdo, Liana Fericean, and Ahmed Ateya. "New Insights on Nucleotide Sequence Variants and mRNA Levels of Candidate Genes Assessing Resistance/Susceptibility to Mastitis in Holstein and Montbéliarde Dairy Cows." Veterinary Sciences 10, no. 1 (2023): 35. http://dx.doi.org/10.3390/vetsci10010035.

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A major factor in the propagation of an infectious disease is host genetics. In this study, 180 dairy cows (90 of each breed: Holstein and Montbéliarde) were used. Each breed’s tested dairy cows were divided into two groups of comparable size (45 cows each), mastitis-free and mastitis-affected groups. Each cow’s jugular vein was punctured to obtain blood samples for DNA and RNA extraction. In the examined Holstein and Montbéliarde dairy cows, single nucleotide polymorphisms (SNPs) related with mastitis resistance/susceptibility were found in the RASGRP1, NFkB, CHL1, MARCH3, PDGFD, MAST3, EPS15
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22

Tian, Hong, Xiaojing Zhou, Hao Wang, et al. "The Prediction of Clinical Mastitis in Dairy Cows Based on Milk Yield, Rumination Time, and Milk Electrical Conductivity Using Machine Learning Algorithms." Animals 14, no. 3 (2024): 427. http://dx.doi.org/10.3390/ani14030427.

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In commercial dairy farms, mastitis is associated with increased antimicrobial use and associated resistance, which may affect milk production. This study aimed to develop sensor-based prediction models for naturally occurring clinical bovine mastitis using nine machine learning algorithms with data from 447 mastitic and 2146 healthy cows obtained from five commercial farms in Northeast China. The variables were related to daily activity, rumination time, and daily milk yield of cows, as well as milk electrical conductivity. Both Z-standardized and non-standardized datasets pertaining to four
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Khurram Ashfaq, Ghulam Muhammad, Arfan Yousaf, Sultan Habibullah Khan, and Sajjad ur Rehman. "Biotyping and PCR Based Detection of Staphylococcus Aureus in Mastitic Large Ruminant Population of District Faisalabad." Indus Journal of Bioscience Research 2, no. 2 (2024): 1601–6. https://doi.org/10.70749/ijbr.v2i02.452.

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S. aureus is considered one of the most important economical disease of dairy enterprise worldwide which affect the quantity and quality of the milk. The present study aimed at detection of S. aureus in mastitic cows and buffaloes using PCR based and biotyping techniques. For this purpose, a total of 374 lactating animals (cows n= 169; buffaloes n= 205) belonging to five small holder and three institutional dairy herds were examined for clinical and subclinical mastitis. Using Surf Field Mastitis Test (SFMT), 14 (8.28%) out of 169 cows and 35(17.07%) out of 205 buffaloes were clinically mastit
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Elsobky, Yumna, Ibrahim Rabah, Walid Mousa, et al. "<b>Isolation and molecular identification of multidrug-resistant <i>Escherichia coli</i> strains isolated from mastitic cows in Egypt</b>." Open Veterinary Journal 15, no. 5 (2025): 2094. https://doi.org/10.5455/ovj.2025.v15.i5.27.

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Background: Mastitis is a common disease that affects the dairy sector globally because it not only impacts animal welfare but can also lead to significant financial losses. Aim: This study examined the phenotypic and genotypic profiles of the multidrug-resistant (MDR) Escherichia coli (E. coli) strains that were isolated from mastitic cows in Egypt to detect their pattern of antibiotic resistance. Methods: Four-hundred native breed lactating cows were evaluated to identify clinical and subclinical mastitis. A total of 100 mastitic milk samples (64 from clinical mastitis and 36 from subclinica
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Ruiz J, Andrés F., Carlos Tobón, and Martha Olivera. "Detección y seguimiento de la mastitis en un hato de ganado Brahman en el trópico bajo colombiano." Revista Colombiana de Ciencias Pecuarias 25, no. 1 (2012): 71–80. http://dx.doi.org/10.17533/udea.rccp.324735.

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In a Brahman cattle farm (573 breeding cows) located in the lowland tropics of Colombia the weight gain of nursing calves decreased with no apparent cause and several cows had non-functional udders. Objective: to assess udder health by means of the presence of subclinical mastitis using CMT or clinical mastitis. Methods: all the cows of the farm were monitored. During 41 months, from September 2002 until January 2006, a total of 8,460 udder quarters were sampled to establish the evolution of mastitis. Cows with clinical or subclinical moderate or severe mastitis were treated with one of five a
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Valdrina, Fetai, Nakov Dimitar, Jashari Besirm, and Trajchev Metodija. "Economics of milk yield losses in one dairy farm in Macedonia associated with clinical mastitis." International Journal of Business & Technology 3, no. 1 (2014): 42–50. http://dx.doi.org/10.33107/ijbte.2014.3.1.06.

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Mastitis remains one of the major disease in dairy herds, causing profound economic losses to the entire milk production chain due to changes in the milk quality and milk yield. A one year cross-sectional longitudinal survey was carried out to evaluate milk yield losses associated with occurrence of clinical mastitis cases in one dairy herd. Cows that had clinical mastitis and clinically healthy cows during lactation were included in the study. The traits analyzed were the average of the monthly test day milk yields, cow parities and days in lactation while first case of clinical mastitis was
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Salat, Olivier, Guillaume Lemaire, Luc Durel, and Florent Perrot. "Etiology of severe mastitis in French dairy herds." PLOS ONE 18, no. 12 (2023): e0295614. http://dx.doi.org/10.1371/journal.pone.0295614.

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Severe clinical mastitis is not so frequent in dairy cows, but it can have a significant economic impact due to its high mortality rate and adverse effects on milk production. Surveys about the cause of mastitis typically provide overall findings without connecting them to a specific medical condition. There are few studies on the specific etiology of severe mastitis. Only etiological results obtained during field studies are available in France, and the number of mastitic milk samples analyzed is always low. In recent years, veterinary clinics have improved their ability to identify bacteria
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FOTSAC DZOUSSE, Müller, Alain KOUAM SIMO, Roland NANKAM CHIMI, et al. "Bibliographic Synthesis on the Influence of Cow Mastitis on Fresh Milk Quality." Clinical Medicine And Health Research Journal 3, no. 3 (2023): 438–43. http://dx.doi.org/10.18535/cmhrj.v3i3.193.

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Mastitis in lactating cows lead to a decrease in the overall cow production; make milk unfit for human and calf consumption; deteriorate milk quality. However, data are not updated on the influence of mastitis on the physicochemical and microbiological quality of milk. Therefore, the present review was made to highlight the current state of knowledge on prior research on the impact of mastitis on milk quality, and to identify the shortcomings of these studies. Two themes were addressed to achieve this. We started by reviewing the prevalence, aetiology and factors associated with the occurrence
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Zafalon, Luiz Francisco, Alessandro Pelegrine Minho, Emanuelle Baldo Gaspar, et al. "Evaluation of homeopathic protocols for the treatment of subclinical mastitis in lactating cows." International Journal of High Dilution Research - ISSN 1982-6206 22, cf (2023): 105–14. http://dx.doi.org/10.51910/ijhdr.v22icf.1278.

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Background: We analyzed the effects of a homeopathic therapies to control subclinical mastitis in two dairy herds. Two experimental herds were used, one in the municipality of São Carlos, SP (Herd A) and the other in Bagé, RS (Herd B), with 46 and 37 lactating animals, respectively. Milk yield, somatic cell count (SCC), and levels of fat, protein, lactose and total solids were evaluated. Milk samples were collected for microbiological confirmation of mastitis. The lactating cows were divided into four groups formed on each of the two farms: cows with mastitis, treated and untreated, and health
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30

Açıkgöz, G., P. Coşkun, Y. Ergün, N. Başpinar, and F. Kazak. "Characterization of normal and mastitic milks using Raman Spectroscopy by application of SERS method." Journal of the Hellenic Veterinary Medical Society 75, no. 2 (2024): 7577–84. http://dx.doi.org/10.12681/jhvms.35310.

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One of the problems of the dairy industry which causes major economic losses due to reduced milk yield and quality, is "Mastitis". Mastitis is a disease that occurs in the glandular tissue of the mammary gland caused by infectious agents. Since milk and milk derived products are consumed by a large part of the World’s population, the quality of milk becames important. Rapid, non-destructive analysis of the milk by Raman Spectroscopy is of great advantage. In the study, it is aimed to investigate the molecular structures of normal and mastitic milks using Raman spectroscopy and this study is th
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Hristov, Slavca, Branislav Stankovic, and Renata Relic. "Clinical and subclinical mastitis in cows." Biotehnologija u stocarstvu 21, no. 1-2 (2005): 29–39. http://dx.doi.org/10.2298/bah0502029h.

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The most important aspects of diagnosis and occurrence of clinical and subclinical mastitis are presented in this paper. Mastitis is inflammation of udders parenchyma characterized by pathological changes, and physical chemical and bacteriological changes of milk. Subclinical mastitis could be diagnosed by direct or indirect somatic cell count (SCC) by California Mastitis test and N-acetyl-B-D-glucosamynidasys test, ELISA and Whiteside test, and also by chloride and bovine serum albumin level, as well as by electric conductivity of milk. Therapy of mastitis is more successful with appropriate
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Bochniarz, Mariola, Tomasz Piech, Tomasz Kocki, Mateusz Iskra, Henryk Krukowski, and Tomasz Jagielski. "Tryptophan, Kynurenine and Kynurenic Acid Concentrations in Milk and Serum of Dairy Cows with Prototheca Mastitis." Animals 11, no. 12 (2021): 3608. http://dx.doi.org/10.3390/ani11123608.

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The aim of this work was to investigate serum and milk levels of tryptophan (TRP), kynurenine (KYN), and kynurenic acid (KYNA), as well as the activity of indoleamine 2,3-dioxygenase (IDO) in cows with mastitis due to Prototheca algae. The study was prompted by previous research showing a link between the KYN pathway of TRP metabolism and bovine mastitis of bacterial etiology. The study was carried out over a 2-year period (2018–2019) and included quarter milk and serum samples collected from six dairy herds in Poland. The samples were obtained from healthy cows and cows with Prototheca mastit
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Ahmad, T., M. Kashif, E. Ahmad, M. Nadeem, and M. Rizwan. "PREVALENCE OF SUBCLINICAL MASTITIS, ASSOCIATED RISK FACTORS AND ANTIBIOTIC SENSITIVITY OF ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS IN CATTLE IN MULTAN, PAKISTAN." Pakistan Journal of Agriculture, Agricultural Engineering and Veterinary Sciences 37, no. 1 (2021): 48–55. http://dx.doi.org/10.47432/2021.37.1.7.

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Mastitis is the most widespread and economically important disease of dairy sector worldwide. The present research was, therefore intended to establish the prevalence of mastitis in and around the Multan district. A total of 100 dairy cows were randomly sampled during the period from July 2018 to October 2018 from different local dairy farms at Multan. After screening with California Mastitis Test, milk samples from mastitic animals were cultured and subjected to microbiological examination.The overall prevalence of subclinical mastitis in cows was 38% and quarter-wise prevalence of subclinica
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Niu, L. L., C. H. Wei, and L. X. Du. "Plasma proteomic profiles of healthy and mastitic cows – host responses to bovine mastitis." Archives Animal Breeding 56, no. 1 (2013): 980–87. http://dx.doi.org/10.7482/0003-9438-56-099.

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Abstract. Mastitis is the most common disease in dairy cows and has resulted in a tremendous economic loss in dairy industry. In the present study, differentially expressed proteins (DEP) were identified among healthy, moderate and severe mastitic cows by proteomic profiling. The health status of cows was closely determined by the somatic cell count (SCC). Differentially expressed proteins were resolved using the two-dimensional gel electrophoresis (2-DE) with the pH 4–7 non-linear DryStrips. Subsequently, 8 protein spots, which altered more than 3-fold, were isolated and identified with the m
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Mohamed, El-Diasty, Talaat Hager, Atwa Samar, Elbaz Elzahraa, and Eissa Mohamed. "Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows." Mansoura Veterinary Medical Journal 20, no. 3 (2019): 35–39. https://doi.org/10.35943/mvmj.2019.23.207.

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<strong>Abstract</strong> <strong><em>Objective:</em></strong> To study the prevalence of Coagulase-negative Staphylococci in clinical and subclinical mastitis in dairy cows. <strong><em>Design:</em></strong> Case-control study. <strong><em>Animals:</em></strong>&nbsp; 415 lactating Holstein cows. <strong><em>Procedures:</em></strong>&nbsp; A total of 896 quarter milk samples were collected aseptically from 415 dairy cows for bacteriological examination (mastitic cows, n=50; healthy cows, n=174). Identification of different bacterial isolates followed by antibiotic sensitivity test for Coagula
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36

Đud, Dalibor, Saša Lončar, Mislav Đidara, and Marcela Šperanda. "Određeni minerali iz hrane i zdravstveno stanje vimena mliječnih krava." Krmiva 65, no. 2 (2023): 77–86. http://dx.doi.org/10.33128/k.65.2.3.

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Mastitis, upala mliječne žlijezde jedna je od tri glavne bolesti koje utječu na profitabilnost proizvođača mlijeka i najskuplja bolest intenzivnog mliječnog govedarstva. Ekonomski gubici nastaju zbog odbacivanja mlijeka, povećanog izlučenja krava, troškova lijekova, rada veterinara i rada samih stočara. Međutim, sve je više dokaza da hranidba može imati značajan učinak na imunosni sustav, čime utječe na incidenciju infekcije i tijek mastitisa. Glavni utjecaj hranidbe na zdravlje vimena je putem supresije imunosnog sustava. Loš sastav obroka ne uzrokuje mastitis, ali može olakšati bakterijama d
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37

Gáspárdy, András, Gil Ismach, Árpád Bajcsy, Gyula Veress, Szilárd Márkus, and István Komlósi. "Evaluation of the on-line electrical conductivity of milk in mastitic dairy cows." Acta Veterinaria Hungarica 60, no. 1 (2012): 145–55. http://dx.doi.org/10.1556/avet.2012.012.

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Mastitis is a persistent, inflammatory reaction of the udder tissue, which entails a decline in potassium, and is also responsible for a higher somatic cell count (SCC) and electrical conductivity (EC) of milk. The measurement of EC is an indirect, rapid method to detect subclinical mastitis from milk. The aim of this study was to analyse the EC of milk throughout the lactation, around the day of mastitis detection, and also to estimate its heritability based on data from a large-scale dairy farm. Shortly after calving the EC value generally decreases; however, it was discovered that from the
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38

Watanabe, Atsushi, Jiro Hirota, Shinya Shimizu, Shigeki Inumaru, and Kazuhiro Kimura. "Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows." Veterinary Medicine International 2012 (2012): 1–8. http://dx.doi.org/10.1155/2012/172072.

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A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μg/quarter/head, but not 10 μg/quarter/head, induced clinical mastitis in three of four cows during the dry-off period, resulting in an elevated rectal temperature, redness and swelling of the mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, and milk clot formation from 1 to 28 days post infusion (PI). In the mammary secretions of the mastitic glands, high levels of IL-8 were sustained from 8 hours to 28 days PI, peaking at 1–3 days PI. The levels of leukocyte-derived elastase and inflammat
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Rahman, M. M., M. Mazilli, G. Pennarossa, et al. "170 NATURALLY OCCURRING CHRONIC MASTITIS COMPROMISES FOLLICULOGENESIS, AFFECTS VASCULARIZATION, AND INTERACTS WITH DIFFERENTIATION FACTOR GDF-9 IN BOVINE OVARIAN STROMA." Reproduction, Fertility and Development 23, no. 1 (2011): 187. http://dx.doi.org/10.1071/rdv23n1ab170.

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Recent studies have suggested an association between reproductive failure and mastitis in lactating dairy cows, but our understanding of how mastitis affects reproduction is still limited. In the present study we investigated the effects of naturally occurring chronic mastitis on the population dynamics of ovarian follicles. Ovaries and milk samples were collected from 74 cows at slaughter. Milk samples from each quarter, were analyzed following National Mastitis Council procedures. Based on the presence of major pathogens and somatic cell count results, animals were sorted in 9 groups, but on
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Filippsen, Laerte Francisco, Fernanda Barros Moreira, Adauto Taiti Sakashita, and Daniéla Regina Bittencourt. "Prevalência da mastite bovina causada por Prototheca zopfii em rebanhos leiteiros, na região norte do Paraná." Ciência Rural 29, no. 1 (1999): 87–89. http://dx.doi.org/10.1590/s0103-84781999000100016.

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Algas do gênero Prototheca têm sido relacionadas como agente etiológico na ocorrência de mastite bovina, entretanto, nenhum caso foi relatado no estado do Paraná. O presente trabalho objetivou determinar a prevalência desta alga, enquanto agente etiológico nas mastites bovinas, em rebanhos leiteiros, na região norte do Paraná. Foram visitadas 20 propriedades produtoras de leite e realizados exames clínicos e teste do CMT (California Mastitis Test) em todos os animais em lactação. A alga Prototheca zopfii foi isolada de dois animais de uma das propriedades. Todas as cepas isoladas de Prototheca
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Muhee, Amatul, Hamidullah Malik, Riyaz A. Bhat, Syed Taifa, Mehak N. B. Azad, and Waseem Rather. "Phagocytic activity and the phagocytic index of milk PMN’s as a marker for diagnosis and monitoring of the therapeutic and prophylactic efficacy of antioxidant formulation in bovine mastitis." Veterinarski arhiv 91, no. 3 (2021): 227–36. http://dx.doi.org/10.24099/vet.arhiv.1108.

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A clinical study was undertaken on bovine mastitis in the Kashmir valley to study the relationship between Phagocytic activity (PA) and the phagocytic index (PI) of milk neutrophils (PMN’s) and bovine mastitis. The PA and PI of milk PMN’s were assessed in mastitic animals and compared with normal, healthy, lactating animals. A significant decrease was found in the values of the PA and PI of milk neutrophils in clinical cases of mastitis as compared to the healthy control group. The utility of anti-oxidants in clinical management of mastitis was measured through the response to treatment with t
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42

Al-kuzaay, Gh K. A., and Q. H. Kshash. "Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province." Al-Qadisiyah Journal of Veterinary Medicine Sciences 12, no. 2 (2013): 101. http://dx.doi.org/10.29079/vol12iss2art263.

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This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).
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43

Karim, Abdulkarim Jafar. "Study on blood gases, Acid-Base balance and the therapeutic evaluation of different protocol of treatment of mastitic cows." Iraqi Journal of Veterinary Medicine 29, no. 2 (2005): 101–11. http://dx.doi.org/10.30539/iraqijvm.v29i2.856.

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This study was planned to reveal the changes in blood gases and acid-basevalues in mastitic cows and to state the intravenous fluid therapy regimentreatment. A herd with a population of 150 lactating cows was screened forclinical and subclinical mastitis for a period of 6 months. Based on inclusion andexclusion criteria, twenty-eight cows with clinical mastitis were divided in threegroups (mastitic not treated (group M), mastitic treated with antibiotic only(group TA) and mastitic treated with antibiotic and fluid therapy (group TAF)and another five healthy cows (group C) served as controls. B
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44

Zigo, F., S. Illia, Š. Halás, et al. "Detection of Some Virulence Factors in Staphylococci Isolated from Mastitic Cows and Ewes." Folia Veterinaria 66, no. 4 (2022): 18–30. http://dx.doi.org/10.2478/fv-2022-0034.

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Abstract About 150 million families around the world are engaged in milk production. However, inflammation of the mammary gland (mastitis) remains a major problem in dairy ruminants that affects the quality of milk worldwide. The aim of this study was the examination of udder health with detection of contagious and environmental pathogens causing mastitis in 960 and 940 dairy cows and ewes, respectively. The presence of selected virulence factors such as: the formation of haemolysis, gelatinase, biofilm, hydrolyse DNA, and resistance to antibiotics with detection of methicillin resistance gene
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Chagunda, Mizeck GG, Torben Larsen, Martin Bjerring та Klaus L. Ingvartsen. "L-lactate dehydrogenase and N-acetyl-β-D-glucosaminidase activities in bovine milk as indicators of non-specific mastitis". Journal of Dairy Research 73, № 4 (2006): 431–40. http://dx.doi.org/10.1017/s0022029906001956.

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Systematic factors affecting the activities of L-lactate dehydrogenase (LDH) and N-acetyl-β-D-glucosaminidase (NAGase) and somatic cell count (SCC), the association between the activities of LDH and NAGase and SCC with respect to udder health status, and the ability of LDH and NAGase to classify cows in udder health categories for early detection of mastitis were studied. A dataset of records from 74 Danish Holstein, 76 Danish Red and 47 Jersey cows on one research farm was used. Cows were grouped into healthy and clinically mastitic. A healthy cow was defined as having no veterinary treatment
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46

Begum, MIA, MS Hossain, M. Ershaduzzaman, and MS Alam. "Epidemiological studies on subclinical mastitis in dairy goats in northern regions of Bangladesh." Bangladesh Journal of Livestock Research 19, no. 1-2 (2016): 112–22. http://dx.doi.org/10.3329/bjlr.v19i1-2.26433.

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A cross sectional study was carried out from June, 2008 to December, 2010 to estimate the prevalence of subclinical mastitis and to determine the responsible bacterial pathogens in lactating dairy goats in northern districts of Bangladesh. A total of 292 goats and 584 udder halves milk samples were screened using California Mastitis Test (CMT). Prevalence of subclinical mastitis at goat level was 56.2% (164/292), and in udder half level it was 33.9% (198/584). The subclinical mastitis prevalence at goat level was high (71.6%) in Jamnapari goats as compared to Black Bengal goats (50.2%), wherea
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47

Naserkheil, Masoumeh, Farzad Ghafouri, Sonia Zakizadeh, et al. "Multi-Omics Integration and Network Analysis Reveal Potential Hub Genes and Genetic Mechanisms Regulating Bovine Mastitis." Current Issues in Molecular Biology 44, no. 1 (2022): 309–28. http://dx.doi.org/10.3390/cimb44010023.

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Mastitis, inflammation of the mammary gland, is the most prevalent disease in dairy cattle that has a potential impact on profitability and animal welfare. Specifically designed multi-omics studies can be used to prioritize candidate genes and identify biomarkers and the molecular mechanisms underlying mastitis in dairy cattle. Hence, the present study aimed to explore the genetic basis of bovine mastitis by integrating microarray and RNA-Seq data containing healthy and mastitic samples in comparative transcriptome analysis with the results of published genome-wide association studies (GWAS) u
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48

Arney, D. R., H. J. Lindsay, S. Bogoro, and C. J. C. Phillips. "A simulation of the effects of changes in the Na and K content of milk during mastitis on the growth of Staphylococcus aureus, Streptococcus uberis and Escherichia coli." Proceedings of the British Society of Animal Science 1998 (1998): 11. http://dx.doi.org/10.1017/s175275620059663x.

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In a mastitic event damage to the epithelial tissue of the mammary gland, and in particular an increase in paracellular permeability allows sodium to enter the milk from the blood (Schaar and Funke, 1986). Normally there is a stepwise gradient of declining sodium concentration from the interstitial fluid to the epithelial cells to the milk (Berga and Neville, 1985). Milk potassium content is reduced in milk of cows with mastitis, but the proportional change is less than that of sodium (Wegner and Stull, 1978). An experiment was conducted to investigate the response of three mastitis pathogens
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49

Arney, D. R., H. J. Lindsay, S. Bogoro, and C. J. C. Phillips. "A simulation of the effects of changes in the Na and K content of milk during mastitis on the growth ofStaphylococcus aureus,Streptococcus uberisandEscherichia coli." Proceedings of the British Society of Animal Science 1998 (1998): 11. http://dx.doi.org/10.1017/s0308229600032244.

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In a mastitic event damage to the epithelial tissue of the mammary gland, and in particular an increase in paracellular permeability allows sodium to enter the milk from the blood (Schaar and Funke, 1986). Normally there is a stepwise gradient of declining sodium concentration from the interstitial fluid to the epithelial cells to the milk (Berga and Neville, 1985). Milk potassium content is reduced in milk of cows with mastitis, but the proportional change is less than that of sodium (Wegner and Stull, 1978). An experiment was conducted to investigate the response of three mastitis pathogens
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50

Garba, Bashiru, S. A. Habibullah, Bashir Saidu, and Nasiru Suleiman. "Effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats." Veterinary World 12, no. 4 (2019): 572–77. http://dx.doi.org/10.14202/vetworld.2019.572-577.

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Aim: This research project investigates the effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats (RSGs). Materials and Methods: In this investigation, 16 clinically and subclinically diagnosed mastitic and 20 non-mastitic RSGs, within Sokoto metropolis, were sampled. Blood samples were collected both in ethylenediaminetetraacetic acid and anticoagulant free sample bottles for hematology and biochemical analysis, respectively. Results: A statistical analysis of the results revealed no significant difference in all the hematological parameters analyzed for both
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