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Journal articles on the topic 'Matrix Scaffold'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Zheng, Le, Shuangshuang Zheng, Zilong Chen, et al. "Preparation and Properties of Decellularized Sheep Kidney Derived Matrix Scaffolds." Journal of Physics: Conference Series 2160, no. 1 (2022): 012014. http://dx.doi.org/10.1088/1742-6596/2160/1/012014.

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Abstract Scaffolds from tissues or organs have nanoscale microstructures. Derived matrix scaffolds prepared by decellularized method can provide more cell attachment sites, which is conducive to cell adhesion, proliferation, differentiation and other physiological activities on scaffolds. In this study, the sheep kidney decellularized matrix scaffold was prepared by the method of decellularization. Due to the poor mechanical properties of the decellularized matrix, the cross linking method was adopted to enhance its mechanical properties. The decellularization efficiency of sheep renal matrix

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AA Ayu Asri Prima Dewi, Komang Trisna Sumadewi, Putu Diah Witari, Fransiscus Fiano Anthony Kerans, Luh Gde Evayanti, and Dewa Ayu Agung Alit Suka Astini. "Macroscopic and microscopic features of pancreatic scaffold generated by SDS-based decellularization using multiple needle injections." Intisari Sains Medis 14, no. 3 (2023): 1028–31. http://dx.doi.org/10.15562/ism.v14i3.1843.

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Background: Pancreatic tissue engineering requires a scaffold in addition to cells and signaling. An adequate scaffold no longer contains cells but retains its extracellular matrix. Biological scaffolds from organ or tissue decellularization are widely used because they can retain the extracellular matrix essential for cell proliferation and differentiation. Pancreatic scaffolds were developed for pancreatic tissue engineering through various decellularization techniques. Available decellularization techniques have advantages and disadvantages, and the development of new techniques is necessar

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Rajagopal, Karthikeyan, Sowmya Ramesh, Noel Malcolm Walter, Aditya Arora, Dhirendra S. Katti, and Vrisha Madhuri. "In vivo cartilage regeneration in a multi-layered articular cartilage architecture mimicking scaffold." Bone & Joint Research 9, no. 9 (2020): 601–12. http://dx.doi.org/10.1302/2046-3758.99.bjr-2019-0210.r2.

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Aims Extracellular matrix (ECM) and its architecture have a vital role in articular cartilage (AC) structure and function. We hypothesized that a multi-layered chitosan-gelatin (CG) scaffold that resembles ECM, as well as native collagen architecture of AC, will achieve superior chondrogenesis and AC regeneration. We also compared its in vitro and in vivo outcomes with randomly aligned CG scaffold. Methods Rabbit bone marrow mesenchymal stem cells (MSCs) were differentiated into the chondrogenic lineage on scaffolds. Quality of in vitro regenerated cartilage was assessed by cell viability, gro

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Kondiah, Pariksha Jolene, Pierre P. D. Kondiah, Yahya E. Choonara, Thashree Marimuthu, and Viness Pillay. "A 3D Bioprinted Pseudo-Bone Drug Delivery Scaffold for Bone Tissue Engineering." Pharmaceutics 12, no. 2 (2020): 166. http://dx.doi.org/10.3390/pharmaceutics12020166.

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A 3D bioprinted pseudo-bone drug delivery scaffold was fabricated to display matrix strength, matrix resilience, as well as porous morphology of healthy human bone. Computer-aided design (CAD) software was employed for developing the 3D bioprinted scaffold. Further optimization of the scaffold was undertaken using MATLAB® software and artificial neural networks (ANN). Polymers employed for formulating the 3D scaffold comprised of polypropylene fumarate (PPF), free radical polymerized polyethylene glycol- polycaprolactone (PEG-PCL-PEG), and pluronic (PF127). Simvastatin was incorporated into th

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Roth, Susanne Pauline, Walter Brehm, Claudia Groß, Patrick Scheibe, Susanna Schubert, and Janina Burk. "Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering." International Journal of Molecular Sciences 20, no. 21 (2019): 5474. http://dx.doi.org/10.3390/ijms20215474.

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Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGFβ3 in an in vitro model. TGFβ3 could effectively be loaded onto tendon scaffolds so that at least 88% of the applied TGFβ3 were not detected in the rinsing fluid of the TGFβ3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) were then seeded on scaffolds loaded wi

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Lari, Alireza, Tao Sun, and Naznin Sultana. "PEDOT:PSS-Containing Nanohydroxyapatite/Chitosan Conductive Bionanocomposite Scaffold: Fabrication and Evaluation." Journal of Nanomaterials 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/9421203.

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Conductive poly(3,4-ethylenedioxythiophene)-poly(4-styrene sulfonate) (PEDOT:PSS) was incorporated into nanohydroxyapatite/chitosan (nHA/CS) composite scaffolds through a freezing and lyophilization technique. The bionanocomposite conductive scaffold was then characterized using several techniques. A scanning electron microscope image showed that the nHA and PEDOT:PSS were dispersed homogeneously in the chitosan matrix, which was also confirmed by energy-dispersive X-ray (EDX) analysis. The conductive properties were measured using a digital multimeter. The weight loss and water-uptake propert

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Wang, Xue Jun, Tao Lou, Jing Yang, Zhen Yang та Kun Peng He. "Preparation of PLLA/HAP/β-TCP Composite Scaffold for Bone Tissue Engineering". Applied Mechanics and Materials 513-517 (лютий 2014): 143–46. http://dx.doi.org/10.4028/www.scientific.net/amm.513-517.143.

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In this study, a nanofibrous poly (L-lactic acid) (PLLA) scaffold reinforced by Hydroxyapatite (HAP) and β-tricalcium phosphate (β-TCP) was fabricated using the thermally induced phase separation method. The composite scaffold morphology showed a nanofibrous PLLA matrix and evenly distributed β-TCP/HAP particles. The composite scaffold had interconnective micropores and the pore size ranged 2-10 μm. Introducing β-TCP/HAP particles into PLLA matrix significantly improved the mechanical properties of the composite scaffold. In summary, the new composite scaffolds show a great deal promise for us

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Wu, Yao Hong, Bao Shan Xu, Qiang Yang, et al. "Fabrication and Evaluation of a Novel Integrated Annulus Fibrosus-Nucleus Pulposus Hybrid Scaffold." Advanced Materials Research 647 (January 2013): 688–91. http://dx.doi.org/10.4028/www.scientific.net/amr.647.688.

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The scope of this study was to fabricate and evaluate a novel integrated annulus fibrosus-nucleus pulposus scaffold composed of bone matrix gelatin (BMG) and cartilage extracellular matrix (ECM) respectively. Scaffolds were fabricated by a serial physicochemical and free-drying process. The physiochemical property and compatibility of the composite scaffold with intervertebral disc cells were evaluated. HE staining showed no residual cells in both annulus fibrosus and nucleus pulposus scaffolds. SEM observation revealed that the integration of annulus fibrosus region and nucleus pulposus regio

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Xiao, Tongguang, Weimin Guo, Mingxue Chen, et al. "Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton’s Jelly Extracellular Matrix." BioMed Research International 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/5839071.

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The scaffold is a key element in cartilage tissue engineering. The components of Wharton’s jelly are similar to those of articular cartilage and it also contains some chondrogenic growth factors, such as insulin-like growth factor I and transforming growth factor-β. We fabricated a tissue-engineered cartilage scaffold derived from Wharton’s jelly extracellular matrix (WJECM) and compared it with a scaffold derived from articular cartilage ECM (ACECM) using freeze-drying. The results demonstrated that both WJECM and ACECM scaffolds possessed favorable pore sizes and porosities; moreover, they s

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Guimaraes, Alberto Bruning, Aristides Tadeu Correia, Ronaldo Soares da Silva, et al. "Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols." Bioengineering 10, no. 5 (2023): 584. http://dx.doi.org/10.3390/bioengineering10050584.

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Tracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold’s biomechanical properties. We tested three protocols for porcine tracheal scaffold preservation immersed in PBS and alcohol 70%, in the fridge and under cryopreservation. Ninety-six porcine tracheas (12 in natura, 84 decellularized) were divided into three groups (PBS, alcohol, and cryopreservation). Twelve tracheas were analyzed af

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Badylak, Stephen, Leslie Geddes, Leslie Geddes, and Joe Obermiller. "Extracellular Matrix for Myocardial Repair." Heart Surgery Forum 6, no. 2 (2005): 20. http://dx.doi.org/10.1532/hsf.917.

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<P>Objective: To evaluate the remodeling characteristics of an extracellular matrix (ECM) scaffold when used as a template for myocardial repair. </P><P>Background: Xenogeneic ECM has been shown to be an effective scaffold for the repair and reconstitution of several tissues, including lower urinary tract structures, dura mater, the esophagus, musculotendinous tissues, and blood vessels. These ECM scaffolds are completely degraded in vivo and induce a host cellular response that supports constructive remodeling rather than scar tissue formation. </P><P>Methods: Fu

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Sotnichenko, A. S., E. A. Gubareva, I. V. Gilevich, et al. "Decellularized rat heart matrix as a basis for creation of tissue engineered heart." Genes & Cells 8, no. 3 (2013): 86–94. http://dx.doi.org/10.23868/gc120548.

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Development of bioengineered scaffolds of internal organs is one of the priority areas of tissue engineering. Decellularization allows to obtain biological (natural) scaffolds while preserving extracellular matrix and three-dimensional structure of organs. The primary goals of the present research were to investigate pathological characteristics of the decellularized rat heart scaffold and evaluate adhesion and viability of multipotent mesenchymal stromal cells (MMSC) during recellularization. Rat hearts were decellularized using a modified detergent-enzymatic method including sodium deoxychol

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Huber, Jessica E., Alan Spievack, Robert L. Ringel, Abby Simmons-Byrd, and Stephen Badylak. "Extracellular Matrix as a Scaffold for Laryngeal Reconstruction." Annals of Otology, Rhinology & Laryngology 112, no. 5 (2003): 428–33. http://dx.doi.org/10.1177/000348940311200508.

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Porcine-derived xenogeneic extracellular matrix (ECM) has been successfully used as a scaffold for tissue repair and reconstruction in numerous preclinical animal studies and human applications. These scaffolds are completely and rapidly degraded and replaced by host-derived tissues that frequently mimic the original tissue composition and architecture. The purpose of the present study was to examine the morphology of ECM scaffolds after their use for laryngeal reconstruction. Thirty adult female dogs were subjected to a partial hemilaryngectomy. The right thyroid cartilage and vocal fold were

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Alnojeidi, Hatem, Ruhangiz Taghi Kilani, and Aziz Ghahary. "Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model." Gels 8, no. 1 (2022): 49. http://dx.doi.org/10.3390/gels8010049.

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(1) Background: Developing a high-quality, injectable biomaterial that is labor-saving, cost-efficient, and patient-ready is highly desirable. Our research group has previously developed a collagen-based injectable scaffold for the treatment of a variety of wounds including wounds with deep and irregular beds. Here, we investigated the biocompatibility of our liquid scaffold in mice and compared the results to a commercially available injectable granular collagen-based product. (2) Methods: Scaffolds were applied in sub-dermal pockets on the dorsum of mice. To examine the interaction between t

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Wang, Guoyong, Chongxian He, Wengjing Yang, et al. "Surface-Modified Graphene Oxide with Compatible Interface Enhances Poly-L-Lactic Acid Bone Scaffold." Journal of Nanomaterials 2020 (March 7, 2020): 1–11. http://dx.doi.org/10.1155/2020/5634096.

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Graphene oxide (GO) usually serves as a reinforce phase in polymer because of its superior mechanical strength and high specific surface area. In this work, GO was grafted with L-lactic acid monomer (denoted as GO@PLLA) to overcome the aggregation in matrix and then incorporated into the poly-L-lactic acid (PLLA) scaffold fabricated by selective laser sintering. In hybrid scaffold, GO@PLLA exhibited uniform dispersion in the matrix. Furthermore, mechanical interlock between GO@PLLA and PLLA matrix formed and reinforced the interface bonding. On the other hand, the heterogeneous distributed GO

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Yang, Shuangjia, Le Zheng, Zilong Chen, et al. "Decellularized Pig Kidney with a Micro-Nano Secondary Structure Contributes to Tumor Progression in 3D Tumor Model." Materials 15, no. 5 (2022): 1935. http://dx.doi.org/10.3390/ma15051935.

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In spite of many anti-cancer drugs utilized in clinical treatment, cancer is still one of the diseases with the highest morbidity and mortality worldwide, owing to the complexity and heterogeneity of the tumor microenvironment. Compared with conventional 2D tumor models, 3D scaffolds could provide structures and a microenvironment which stimulate native tumor tissues more accurately. The extracellular matrix (ECM) is the main component of the cell in the microenvironment that is mainly composed of three-dimensional nanofibers, which can form nanoscale fiber networks, while the decellularized e

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Goswami, Riddhi, Subhrojyoti Ghosh, Shuvayan Dasgupta, et al. "Recent Advances in Tissue Engineering Scaffolds and Commercial Applications." YMER Digital 21, no. 06 (2022): 865–82. http://dx.doi.org/10.37896/ymer21.06/86.

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Scaffolds for tissue engineering are support structures that help cells grow and multiply after being implanted into a patient. To allow cellular adhesion, proliferation, and differentiation, the optimal scaffolds should have the right surface chemistry and microstructures. Furthermore, the scaffolds must have sufficient mechanical strength and a low rate of biodegradation with no unwanted by-products. Regenerative medicine efforts currently rely on the transplantation of cells in combination with supporting scaffolds and macromolecules to restore pathologically damaged tissue architectures. B

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Barreto, Rodrigo SN, Patricia Romagnolli, Paula Fratini, Andrea Maria Mess, and Maria Angelica Miglino. "Mouse placental scaffolds: a three-dimensional environment model for recellularization." Journal of Tissue Engineering 10 (January 2019): 204173141986796. http://dx.doi.org/10.1177/2041731419867962.

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The rich extracellular matrix (ECM) and availability make placenta eligible as alternative biomaterial source. Herein we produced placental mouse scaffolds by decellularization, and structure, composition, and cytocompatibility were evaluated to be considered as a biomaterial. We obtained a cell-free scaffold containing 9.42 ± 5.2 ng dsDNA per mg of ECM, presenting well-preserved structure and composition. Proteoglycans were widespread throughout ECM without cell nuclei and cell remnants. Collagen I, weak in native placenta, clearly appears in the scaffold after recellularization, opposite dis

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Zhang, Xing. "In-situ polarized scaffold activated by ultrasound cycle." Journal of Physics: Conference Series 2954, no. 1 (2025): 012046. https://doi.org/10.1088/1742-6596/2954/1/012046.

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Abstract Intervention measures are essential in clinically treating significant bone defects or poor osteogenic conditions. Although autologous transplantation is the preferred method, it has the risk of secondary injury and infection. Bone tissue engineering technology uses biomaterial scaffolds and biochemical and biophysical signals to regulate cell behaviour and promote tissue repair. The Endogenous electric field is vital in tissue regeneration, especially in bone. The piezoelectric effect of collagen matrix is closely related to bone growth and remodelling. This study prepared PCL/PTFE c

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Ching, Kuan Yong, Orestis Andriotis, Bram Sengers, and Martin Stolz. "Genipin crosslinked chitosan/PEO nanofibrous scaffolds exhibiting an improved microenvironment for the regeneration of articular cartilage." Journal of Biomaterials Applications 36, no. 3 (2021): 503–16. http://dx.doi.org/10.1177/08853282211002015.

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Towards optimizing the growth of extracellular matrix to produce repair cartilage for healing articular cartilage (AC) defects in joints, scaffold-based tissue engineering approaches have recently become a focus of clinical research. Scaffold-based approaches by electrospinning aim to support the differentiation of chondrocytes by providing an ultrastructure similar to the fibrillar meshwork in native cartilage. In a first step, we demonstrate how the blending of chitosan with poly(ethylene oxide) (PEO) allows concentrated chitosan solution to become electrospinnable. The chitosan-based scaffo

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Walawalkar, Sonal, Mahesh Kumar Verma, and Shahdab Almelkar. "Re-endothelization of human saphenous vein scaffold surfaces for bioprosthesis fabrication." Journal of Biomaterials Applications 34, no. 8 (2020): 1081–91. http://dx.doi.org/10.1177/0885328219898349.

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Various in vitro methods have been used for biological and synthetic scaffold fabrication. Some use polymers such as expanded polytetrafluoroethylene (ePTFE), polytetrafluoroethylene (PTFE), and polyethylene terephthalate (PET), while others use allogeneic or xenogeneic biological materials (e.g. blood vessels). While fabricating a biological scaffold, the first step is complete decellularization by enzymes (e.g. trypsin, collagenase, etc.) or chemicals (e.g. SDS, Triton-X, etc.), and the scaffolds should maintain its extracellular matrix (ECM). The second step involves re-endothelization so a

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Taghavi, Hossein, Jafar Soleimani Rad, Ahmad Mehdipour, et al. "Effect of Mineral Pitch on the Proliferation of Human Adipose Derived Stem Cells on Acellular Scaffold." Advanced Pharmaceutical Bulletin 10, no. 4 (2020): 623–29. http://dx.doi.org/10.34172/apb.2020.075.

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Purpose : Acellular scaffold extracted from extracellular matrix (ECM) have been used for constructive and regenerative medicine. Adipose derived stem cells (ADSCs) can enhance the vascularization capacity of scaffolds. High mobility group box 1 (HMGB1) and stromal derived factor1 (SDF1) are considered as two important factors in vascularization and immunologic system. In this study, the effect of mineral pitch on the proliferation of human ADSCs was evaluated. In addition to HMGB1 and SDF1, factors expression in acellular scaffold was also assessed. Methods: To determine acellular scaffold mo

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Adachi, Tetsuya, Nao Miyamoto, Hayata Imamura, et al. "Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation." International Journal of Molecular Sciences 23, no. 15 (2022): 8099. http://dx.doi.org/10.3390/ijms23158099.

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This study presents a set of vibrational characterizations on a nanogel-cross-linked porous freeze-dried gel (NanoCliP-FD gel) scaffold for tissue engineering and regenerative therapy. This scaffold is designed for the in vitro culture of high-quality cartilage tissue to be then transplanted in vivo to enable recovery from congenital malformations in the maxillofacial area or crippling jaw disease. The three-dimensional scaffold for in-plate culture is designed with interface chemistry capable of stimulating cartilage formation and maintaining its structure through counteracting the dedifferen

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Huang, Ching-Cheng. "Characteristics and Preparation of Designed Alginate-Based Composite Scaffold Membranes with Decellularized Fibrous Micro-Scaffold Structures from Porcine Skin." Polymers 13, no. 20 (2021): 3464. http://dx.doi.org/10.3390/polym13203464.

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Alginate-based composite scaffold membranes with various ratios of decellularized extracellular matrices could be designed and obtained from porcine skin tissue by using supercritical carbon dioxide fluid technology. Retention of decellularized extracellular matrix (dECM) and scaffold-structure integrity was observed. This work provides a simple and time-saving process for the preparation of biomedical alginate-based composite scaffold membranes with fibrous dECM micro-scaffolds, which were further characterized by Fourier transform infrared spectroscopy (FTIR), thermo-gravimetric analysis (TG

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Figliuzzi, Marina, Barbara Bonandrini, and Andrea Remuzzi. "Decellularized Kidney Matrix as Functional Material for whole Organ Tissue Engineering." Journal of Applied Biomaterials & Functional Materials 15, no. 4 (2017): e326-e333. http://dx.doi.org/10.5301/jabfm.5000393.

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Renal transplantation is currently the most effective treatment for end-stage renal disease, which represents one of the major current public health problems. However, the number of available donor kidneys is drastically insufficient to meet the demand, causing prolonged waiting lists. For this reason, tissue engineering offers great potential to increase the pool of donated organs for kidney transplantation, by way of seeding cells on supporting scaffolding material. Biological scaffolds are prepared by removing cellular components from the donor organs using a decellularization process with

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Zhao, Jing, Rui Liu, Jing Zhu, Shulan Chen, Jianxin Liu, and Ling Xu. "Preparation of three-dimensional matrices of human gingival tissue and comparison of induction of mesenchymal and blastema stem cell behaviors in prepared scaffolds." Cellular and Molecular Biology 68, no. 1 (2022): 102–8. http://dx.doi.org/10.14715/cmb/2022.68.1.13.

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This study aimed to compare the behavior of rat bone marrow mesenchymal stem cells and rabbit auricle blastema cells implanted in non-cellular gingival tissue scaffold of human. In this regard, the tissues obtained from gingival surgeries in the dental clinic were de-cellulated using two detergents of sodium dodecyl sulfate and triton 100-X. After washing and sterilization, they were used as a scaffold for culture with bone marrow mesenchymal stem cells of rats. Using light and electron microscopy, these scaffolds were examined before and after 1, 2, and 4 weeks of cell culture. Also, the prep

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Risbud, Makarand V., Erdal Karamuk, René Moser, and Joerg Mayer. "Hydrogel-Coated Textile Scaffolds as Three-Dimensional Growth Support for Human Umbilical Vein Endothelial Cells (HUVECs): Possibilities as Coculture System in Liver Tissue Engineering." Cell Transplantation 11, no. 4 (2002): 369–77. http://dx.doi.org/10.3727/000000002783985837.

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Three-dimensional (3-D) scaffolds offer an exciting possibility to develop cocultures of various cell types. Here we report chitosan–collagen hydrogel-coated fabric scaffolds with defined mesh size and fiber diameter for 3-D culture of human umbilical vein endothelial cells (HUVECs). These scaffolds did not require pre-coating with fibronectin and they supported proper HUVEC attachment and growth. Scaffolds preserved endothelial cell-specific cobblestone morphology and cells were growing in compartments defined by the textile mesh. HUVECs on the scaffold maintained the property of contact inhi

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Tasnim, Afsara, Diego Jacho, Agustin Rabino, et al. "Immunomodulation Through Fibroblast-Derived Extracellular Vesicles (EVs) Within 3D Polycaprolactone–Collagen Matrix." Biomimetics 10, no. 8 (2025): 484. https://doi.org/10.3390/biomimetics10080484.

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Extracellular vesicles (EVs) have emerged as promising acellular tools for modulating immune responses for tissue engineering applications. This study explores the potential of human fibroblast-derived EVs delivered within a three-dimensional (3D) injectable scaffold composed of polycaprolactone (PCL) nanofibers and collagen (PNCOL) to reprogram macrophage behavior and support scaffold integrity under inflammatory conditions. EVs were successfully isolated from human fibroblasts using ultracentrifugation and characterized for purity, size distribution and surface markers (CD63 and CD9). Macrop

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Dewey, Marley J., Eileen M. Johnson, Simona T. Slater, Derek J. Milner, Matthew B. Wheeler, and Brendan A. C. Harley. "Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions." Regenerative Biomaterials 7, no. 3 (2020): 247–58. http://dx.doi.org/10.1093/rb/rbaa005.

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Abstract Defects in craniofacial bones occur congenitally, after high-energy impacts, and during the course of treatment for stroke and cancer. These injuries are difficult to heal due to the overwhelming size of the injury area and the inflammatory environment surrounding the injury. Significant inflammatory response after injury may greatly inhibit regenerative healing. We have developed mineralized collagen scaffolds that can induce osteogenic differentiation and matrix biosynthesis in the absence of osteogenic media or supplemental proteins. The amniotic membrane is derived from placentas

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Gunes, Oylum Colpankan, Aylin Ziylan Albayrak, Seyma Tasdemir, and Aylin Sendemir. "Wet-electrospun PHBV nanofiber reinforced carboxymethyl chitosan-silk hydrogel composite scaffolds for articular cartilage repair." Journal of Biomaterials Applications 35, no. 4-5 (2020): 515–31. http://dx.doi.org/10.1177/0885328220930714.

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The objective of the study was to produce three-dimensional and porous nanofiber reinforced hydrogel scaffolds that can mimic the hydrated composite structure of the cartilage extracellular matrix. In this regard, wet-electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber reinforced carboxymethyl chitosan-silk fibroin (PNFs/CMCht-SF) hydrogel composite scaffolds that were chemically cross-linked by poly(ethylene glycol) diglycidyl ether (PEGDE) were produced. To the best of our knowledge, this is the first study in cartilage regeneration where a three dimensional porous spon

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Rønning, Sissel B., Ragnhild S. Berg, Vibeke Høst, et al. "Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering." International Journal of Molecular Sciences 21, no. 21 (2020): 8130. http://dx.doi.org/10.3390/ijms21218130.

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The purpose of this study was to investigate the tissue regenerating and biomechanical properties of processed eggshell membrane powder (PEP) for use in 3D-scaffolds. PEP is a low-cost, natural biomaterial with beneficial bioactive properties. Most importantly, this material is available as a by-product of the chicken egg processing (breaking) industry on a large scale, and it could have potential as a low-cost ingredient for therapeutic scaffolds. Scaffolds consisting of collagen alone and collagen combined with PEP were produced and analyzed for their mechanical properties and the growth of

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Valdoz, Jonard Corpuz, Benjamin C. Johnson, Dallin J. Jacobs, et al. "The ECM: To Scaffold, or Not to Scaffold, That Is the Question." International Journal of Molecular Sciences 22, no. 23 (2021): 12690. http://dx.doi.org/10.3390/ijms222312690.

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The extracellular matrix (ECM) has pleiotropic effects, ranging from cell adhesion to cell survival. In tissue engineering, the use of ECM and ECM-like scaffolds has separated the field into two distinct areas—scaffold-based and scaffold-free. Scaffold-free techniques are used in creating reproducible cell aggregates which have massive potential for high-throughput, reproducible drug screening and disease modeling. Though, the lack of ECM prevents certain cells from surviving and proliferating. Thus, tissue engineers use scaffolds to mimic the native ECM and produce organotypic models which sh

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Pankajakshan, Divya, and Devendra K. Agrawal. "Scaffolds in tissue engineering of blood vessels." Canadian Journal of Physiology and Pharmacology 88, no. 9 (2010): 855–73. http://dx.doi.org/10.1139/y10-073.

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Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of s

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Doll, Carla U., Sabine Niebert, and Janina Burk. "Mesenchymal Stromal Cells Adapt to Chronic Tendon Disease Environment with an Initial Reduction in Matrix Remodeling." International Journal of Molecular Sciences 22, no. 23 (2021): 12798. http://dx.doi.org/10.3390/ijms222312798.

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Tendon lesions are common sporting injuries in humans and horses alike. The healing process of acute tendon lesions frequently results in fibrosis and chronic disease. In horses, local mesenchymal stromal cell (MSC) injection is an accepted therapeutic strategy with positive influence on acute lesions. Concerning the use of MSCs in chronic tendon disease, data are scarce but suggest less therapeutic benefit. However, it has been shown that MSCs can have a positive effect on fibrotic tissue. Therefore, we aimed to elucidate the interplay of MSCs and healthy or chronically diseased tendon matrix

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Zhai, Chenjun, Xiao Zhang, Jun Chen, et al. "The effect of cartilage extracellular matrix particle size on the chondrogenic differentiation of bone marrow mesenchymal stem cells." Regenerative Medicine 14, no. 7 (2019): 663–80. http://dx.doi.org/10.2217/rme-2018-0082.

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Aim: To investigate the effect of cartilage extracellular matrix (ECM) particle size on the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Materials & methods: BMSCs were seeded into the scaffolds fabricated by small particle ECM materials and large particle ECM materials. For the positive control, chondrogenically induced BMSCs were seeded into commercial poly-lactic-glycolic acid scaffolds. Macroscopic observation, histological and immunohistochemical staining, mechanical testing and biochemical analysis were performed to the cell-scaffold constructs.

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ZHAO, Yan-hong, Qiang YANG, Qun XIA, et al. "In vitro cartilage production using an extracellular matrix-derived scaffold and bone marrow-derived mesenchymal stem cells." Chinese Medical Journal 126, no. 16 (2013): 3130–37. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20130212.

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Background Cartilage repair is a challenging research area because of the limited healing capacity of adult articular cartilage. We had previously developed a natural, human cartilage extracellular matrix (ECM)-derived scaffold for in vivo cartilage tissue engineering in nude mice. However, before these scaffolds can be used in clinical applications in vivo, the in vitro effects should be further explored. Methods We produced cartilage in vitro using a natural cartilage ECM-derived scaffold. The scaffolds were fabricated by combining a decellularization procedure with a freeze-drying technique

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Feng, Guiyu, Wei Liu, Yao Yu, et al. "Angiogenesis coupled with osteogenesis in a bone tissue engineering scaffold enhances bone repair in osteoporotic bone defects." Biomedical Materials 18, no. 4 (2023): 045002. http://dx.doi.org/10.1088/1748-605x/accf55.

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Abstract Increased life expectancy has resulted in an increase in osteoporosis incidence worldwide. The coupling of angiogenesis and osteogenesis is indispensable for bone repair. Although traditional Chinese medicine (TCM) exerts therapeutic effects on osteoporosis, TCM-related scaffolds, which focus on the coupling of angiogenesis and osteogenesis, have not yet been used for the treatment of osteoporotic bone defects. Panax notoginseng saponin (PNS), the active ingredient of Panax notoginseng, was added to a poly (L-lactic acid) (PLLA) matrix. Osteopractic total flavone (OTF), the active ing

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Wang, Ming Bo, Jian Xiong, Bin Chu, Rong Wei Tan, Wei Huang, and Zhen Ding She. "Study on Artificial Bone Scaffolds with Control Release of Drugs by Low-Temperature Rapid Prototyping Technology." Advanced Materials Research 647 (January 2013): 269–77. http://dx.doi.org/10.4028/www.scientific.net/amr.647.269.

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A kind of PLGA microspheres was prepared with bovine serum albumin (BSA) as the model drug and poly (lactide-co-glycolide) as the matrix. The polylactic acid/hydroxyapatite (PLA/HA) scaffold was manufactured through 3D printing technology. Then the PLGA microspheres were composited in the scaffold. It was also explored about the feasibility of skeletal scaffolds loaded with bone growth factor. The BSA loading PLGA microspheres were prepared by W/O/W method and the scaffolds were prepared by 3D-printing using PLA and HA as raw materials. The composite scaffold was fabricated by adsorbing the mi

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Wang, Xin Hui, Lin Sang, Zhi Yong Wei, Li Jie Zhai, and Min Qi. "Fabrication and Characterization of P34HB Scaffolds with Oriented Microtubules." Advanced Materials Research 898 (February 2014): 318–21. http://dx.doi.org/10.4028/www.scientific.net/amr.898.318.

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Sponge-like scaffold with a specific three-dimensional structure resembling the actual extracellular matrix of a particular tissue show significant potential for the regeneration and repair of damaged anisotropic tissues. In this research, an oriented microtubular P34HB scaffold was prepared successfully. The mechanical property showed that anisotropy of modulus is much greater than a typical non-oriented scaffold. Altering the P34HB concentration allowed P34HB scaffolds to be produced with complex pore orientations, and anisotropy in pore size and alignment.

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Aurora, Amit, Benjamin T. Corona, and Thomas J. Walters. "A Porcine Urinary Bladder Matrix Does Not Recapitulate the Spatiotemporal Macrophage Response of Muscle Regeneration after Volumetric Muscle Loss Injury." Cells Tissues Organs 202, no. 3-4 (2016): 189–201. http://dx.doi.org/10.1159/000447582.

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Volumetric muscle loss (VML) results in irrecoverable loss of muscle tissue making its repair challenging. VML repair with acellular extracellular matrix (ECM) scaffolds devoid of exogenous cells has shown improved muscle function, but limited de novo muscle fiber regeneration. On the other hand, studies using minced autologous and free autologous muscle grafts have reported appreciable muscle regeneration. This raises the fundamental question whether an acellular ECM scaffold can orchestrate the spatiotemporal cellular events necessary for appreciable muscle fiber regeneration. This study com

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Wongwitwichot, P., J. Kaewsrichan, K. H. Chua, and B. H. I. Ruszymah. "Comparison of TCP and TCP/HA Hybrid Scaffolds for Osteoconductive Activity." Open Biomedical Engineering Journal 4, no. 1 (2010): 279–85. http://dx.doi.org/10.2174/1874120701004010279.

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Two types of porous ceramic scaffolds were prepared, consisting of β-tricalcium phosphate (TCP) or the mixed powder of TCP and hydroxyapatite (HA) at a 2:1 mass ratio. A variety of methods have been used to fabricate bone scaffolds, while the sintering approach was adopted in this work. An extremely high temperature was used on sintering that proposed to consolidate the ceramic particles. As revealed by SEM, a well opened pore structure was developed within the scaffolds. The θ-values were measured to be of 73.3° and 6.5° for the composite scaffold and TCP sample, respectively. According to XR

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Dong, Xue, Ishani D. Premaratne, Jaime L. Bernstein, et al. "Three-Dimensional-Printed External Scaffolds Mitigate Loss of Volume and Topography in Engineered Elastic Cartilage Constructs." CARTILAGE 13, no. 2_suppl (2021): 1780S—1789S. http://dx.doi.org/10.1177/19476035211049556.

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Objective A major obstacle in the clinical translation of engineered auricular scaffolds is the significant contraction and loss of topography that occur during maturation of the soft collagen-chondrocyte matrix into elastic cartilage. We hypothesized that 3-dimensional-printed, biocompatible scaffolds would “protect” maturing hydrogel constructs from contraction and loss of topography. Design External disc-shaped and “ridged” scaffolds were designed and 3D-printed using polylactic acid (PLA). Acellular type I collagen constructs were cultured in vitro for up to 3 months. Collagen constructs s

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Chin, Jiah Shin, Leigh E. Madden, Anthony R. J. Phillips, Sing Yian Chew, and David L. Becker. "Bio-Mimicking Acellular Wet Electrospun Scaffolds Promote Accelerated Integration and Re-Epithelialization of Full-Thickness Dermal Wounds." Bioengineering 9, no. 7 (2022): 324. http://dx.doi.org/10.3390/bioengineering9070324.

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Scaffolds can promote the healing of burns and chronic skin wounds but to date have suffered from issues with achieving full skin integration. Here, we characterise the wound response by both tissue integration and re-epithelialization to a scaffold using wet electrospinning to fabricate 3D fibrous structures. Two scaffold materials were investigated: poly(ε-caprolactone) (PCL) and PCL + 20% rat tail type 1 collagen (PCL/Coll). We assessed re-epithelisation, inflammatory responses, angiogenesis and the formation of new extracellular matrix (ECM) within the scaffolds in rat acute wounds. The 3D

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Sayin, Esen, Erkan Türker Baran, Ahmed Elsheikh, Vivek Mudera, Umber Cheema, and Vasif Hasirci. "Evaluating Oxygen Tensions Related to Bone Marrow and Matrix for MSC Differentiation in 2D and 3D Biomimetic Lamellar Scaffolds." International Journal of Molecular Sciences 22, no. 8 (2021): 4010. http://dx.doi.org/10.3390/ijms22084010.

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The physiological O2 microenvironment of mesenchymal stem cells (MSCs) and osteoblasts and the dimensionality of a substrate are known to be important in regulating cell phenotype and function. By providing the physiologically normoxic environments of bone marrow (5%) and matrix (12%), we assessed their potential to maintain stemness, induce osteogenic differentiation, and enhance the material properties in the micropatterned collagen/silk fibroin scaffolds that were produced in 2D or 3D. Expression of osterix (OSX) and vascular endothelial growth factor A (VEGFA) was significantly enhanced in

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Alqahtani, Q., S. H. Zaky, A. Patil, E. Beniash, H. Ray, and C. Sfeir. "Decellularized Swine Dental Pulp Tissue for Regenerative Root Canal Therapy." Journal of Dental Research 97, no. 13 (2018): 1460–67. http://dx.doi.org/10.1177/0022034518785124.

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In the current theme of dental pulp regeneration, biological and synthetic scaffolds are becoming a potential therapy for pulp revitalization. The goal is to provide a suitable environment for cellular infiltration, proliferation, and differentiation. The extracellular matrix (ECM) represents a natural scaffold material resembling the native tissue chemical and mechanical properties. In the past few years, ECM-based scaffolds have shown promising results in terms of progenitor cells recruitment, promotion of constructive remodeling, and modulation of host response. These properties make ECM-de

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Sevostyanova, V. V., A. S. Golovkin, L. V. Antonova, T. V. Glushkova, O. L. Barbarash, and L. S. Barbarash. "Modification of polycaprolactone scaffolds with vascular endothelial growth factors for potential application in development of tissue engineered vascular grafts." Genes & Cells 10, no. 1 (2015): 91–97. http://dx.doi.org/10.23868/gc120499.

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In this study, we investigated a biological activity of nonwoven polycaprolactone scaffolds for controlled delivery of vascular endothelial growth factors. The tube scaffolds with incorporated vascular endothelial growth factors were fabricated by method of electrospinning. The polycaprolactone scaffold containing growth factor provided a morphology similar to the native extracellular matrix. The sustained release of biologically active growth factor from scaffold was observed for 80 days The assessment of adhesion and proliferation of multipotent mesenchymal stem cells and endothelial cells o

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Lim, Mim Mim, Tao Sun, and Naznin Sultana. "In VitroBiological Evaluation of Electrospun Polycaprolactone/Gelatine Nanofibrous Scaffold for Tissue Engineering." Journal of Nanomaterials 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/303426.

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The fabrication of biocompatible and biodegradable scaffolds which mimic the native extracellular matrix of tissues to promote cell adhesion and growth is emphasized recently. Many polymers have been utilized in scaffold fabrication, but there is still a need to fabricate hydrophilic nanosized fibrous scaffolds with an appropriate degradation rate for skin tissue engineering applications. In this study, nanofibrous scaffolds of a biodegradable synthetic polymer, polycaprolactone (PCL), and blends of PCL with a natural polymer, gelatine (Ge), in three different compositions: 85 : 15, 70 : 30, a

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Ribas, Montanheiro, Montagna, Prado, Campos, and Thim. "Water Uptake in PHBV/Wollastonite Scaffolds: A Kinetics Study." Journal of Composites Science 3, no. 3 (2019): 74. http://dx.doi.org/10.3390/jcs3030074.

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a widely studied polymer and it has been found that porous PHBV materials are suitable for substrates for cell cultures. A crucial factor for scaffolds designed for tissue engineering is the water uptake. This property influences the transport of water and nutrients into the scaffold, which promotes cell growth. PHBV has significant hydrophobicity, which can harm the production of cells. Thus, the addition of α-wollastonite (WOL) can modify the PHBV scaffold’s water uptake. To our knowledge, a kinetics study of water uptake of α-wollastoni

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Malagón-Escandón, Alda, Mathieu Hautefeuille, Edgar Jimenez-Díaz, et al. "Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis." Polymers 13, no. 24 (2021): 4390. http://dx.doi.org/10.3390/polym13244390.

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The use of three-dimensional porous scaffolds derived from decellularized extracellular matrix (ECM) is increasing for functional repair and regeneration of injured bone tissue. Because these scaffolds retain their native structures and bioactive molecules, in addition to showing low immunogenicity and good biodegradability, they can promote tissue repair and regeneration. Nonetheless, imitating these features in synthetic materials represents a challenging task. Furthermore, due to the complexity of bone tissue, different processes are necessary to maintain these characteristics. We present a

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Heng, Christian Hwee Yee, and Yee Han Dave Lee. "Single-Stage Arthroscopic Cartilage Repair With Gel Scaffold and BMAC." Video Journal of Sports Medicine 1, no. 3 (2021): 263502542110081. http://dx.doi.org/10.1177/26350254211008190.

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Background: Injectable scaffold augmentation has been gaining traction as a promising modality for single-stage cartilage repair. It involves the use of a biological scaffold that augments microfracture techniques by aiding in clot stabilization and maturation. The scaffold provides a matrix that helps with mesenchymal stem cell (MSC) retention and encourages differentiation along a chondrogenic lineage. Bone marrow aspirate concentrate (BMAC) has also been proposed as an alternative source of MSCs to microfracture, and it can potentially avoid the pitfalls of microfracture techniques. Indicat

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