Academic literature on the topic 'Maximum absorbance'

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Journal articles on the topic "Maximum absorbance"

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Brewster, Jeffrey D., and James L. Anderson. "On Absorbance Measurements in Spatially Inhomogeneous Fields." Applied Spectroscopy 43, no. 4 (1989): 710–14. http://dx.doi.org/10.1366/0003702894202427.

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The measured absorbance of a spatially inhomogeneous sample may be strongly affected by the relative spatial distribution of the absorbing medium and the optical beam. The measured absorbance is, in general, not the average of local absorbances across the beam, except when the maximum range of local absorbance values across the entire field is small (<0.1 AU). The measured transmittance of an optically inhomogeneous field is equal to the average transmittance only if incident intensity is everywhere uniform. The general problem is outlined and is illustrated by examples of transient spectro
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Bingjian, Yuan. "Application of the Maximum Sensitivity of the Photometric Method." Science Discovery 13, no. 2 (2025): 33–38. https://doi.org/10.11648/j.sd.20251302.13.

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The method of high content determination of photometry was optimized in the way of maximum sensitivity:1) Under strictly observing the “condition of repeatable measurement”, make the “calibration curve” of the method used .The line joining its upper and lower end points is to be taken as the "calibration line" for the sample, the equation of which is the "two point form ”of the geometry, which indicating that "the change △A in the absorbance of a substance, is directly proportional to the change △C in its substance content, i.e. △A=k△C", and the sen
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Wu, Dan, and Kai Xiao Zhang. "The Linear Relationship between Concentrations and UV Absorbance of Nitrobenzene." Advanced Materials Research 955-959 (June 2014): 1376–79. http://dx.doi.org/10.4028/www.scientific.net/amr.955-959.1376.

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This paper researched the linear relationship between UV absorbance and concentrations of nitrobenzene. It used the UV-1700 UV/VIS spectroscopy to get the absorbance diagram and analyze it. Then it calculated the absorbance of maximum absorption peak and the absorbance integration in a wavelength interval. There is a linear relationship between absorbance and concentration, the correlation coefficient is 0.981 of the maximum absorption peaks and the concentrations, and the largest correlation coefficient between concentrations and the integration of absorbance is 0.995 with the wavelength inte
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Lumbessy, Mirna, Jemmy Abidjulu, and Jessy J. E. Paendong. "Uji Total Flavonoid Pada Beberapa Tanaman Obat Tradisonal Di Desa Waitina Kecamatan Mangoli Timur Kabupaten Kepulauan Sula Provinsi Maluku Utara." Jurnal MIPA 2, no. 1 (2013): 50. http://dx.doi.org/10.35799/jm.2.1.2013.766.

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Penelitian ini bertujuan untuk menguji total kandungan flavonoid pada beberapa tanaman obat tradisional. Penentuan kandungan total flavonoid dilakukan dengan menggunakan metode etanol – HCl, sedangkan analisis flavonoid menggunakan Metode spektrofotometeyr UV-VIS . Hasil yang diperoleh menunjukan kandungan total flavonoid pada tanaman ketepeng cina (Cassia alata L) sebesar 26.8633 mg/mL , iler (Coleus scutellariodes L Benth) sebesar 14.425 mg/mL , rumput teki (Cyperus rotundus L) sebesar 6.505 mg/mL, pegagan (Centella asiatica) sebesar 3.816 mg/mL, rumput mutiara (Oldenlandia corymbosa) sebesa
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García Bermúdez, G., V. C. Chappa, M. F. del Grosso, and R. O. Mazzei. "Stopping power dependence of maximum grafting yield and absorbance measurements." Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 266, no. 12-13 (2008): 3063–66. http://dx.doi.org/10.1016/j.nimb.2008.03.163.

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Mardison, NFN, Usman Ahmad, NFN Sutrisno, and Slamet Widodo. "KARAKTERISASI ABSORBANSI LARUTAN DAN PENDUGAAN DERAJAT SOSOH BERAS BERDASARKAN ABSORBANSI PADA SPEKTRUM ULTRA- VIOLET." Jurnal Penelitian Pascapanen Pertanian 15, no. 1 (2019): 43. http://dx.doi.org/10.21082/jpasca.v15n1.2018.43-51.

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<p>Teknologi non-destruktif seperti penggunaan gelombang ultra-violet (UV) dapat dijadikan sebagai alternatif dalam menentukan kualitas beras sosoh. Pengembangan metode pengukuran dan karakterisasi beras sosoh berdasarkan absorbansi spektrumnya pada daerah UV sangat berpotensi dalam evaluasi kualitas beras secara non-destruktif. Penelitian ini bertujuan untuk menganalisis spektrum absorbansi UV pada beberapa varietas beras dengan tingkat penyosohan bervariasi dan menentukan hubungan derajat sosoh beras varietas Ciherang dengan spektrum absorbansi UV dari larutan beras dalam pelarut n-hek
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Putri, Yulia Eka, and Mulda Muldarisnur. "Pengaruh Frekuensi Vibrasi Terhadap Kualitas Lapisan Tipis Opal yang Disintesis dengan Metode Deposisi Horizontal." Jurnal Fisika Unand 9, no. 1 (2020): 9–16. http://dx.doi.org/10.25077/jfu.9.1.9-16.2020.

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Telah dilakukan sintesis dan karakterisasi lapisan tipis opal menggunakan metode deposisi horizontal dengan penambahan frekuensi vibrasi. Sintesis dilakukan pada suhu ruang dengan variasi frekuensi vibrasi 75 Hz, 200 Hz, 300 Hz, dan 400 Hz menggunakan speaker dalam keadaan volume tetap. Karakterisasi dilakukan menggunakan Mikroskop Optik, SEM (Scanning Electron Microscopy) dan Spektrofotometer UV-Vis. Hasil mikroskop optik menunjukkan permukaan sampel dengan frekuensi vibrasi rendah lebih homogen dan tebal dibandingkan frekuensi vibrasi tinggi. Hasil SEM menunjukkan lapisan multilayer terbentu
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Angelats-Silva, Luis M., David Asmat-Campos, Henry León-León, Kevin A. Wilkinson, Daniel A. Sánchez-Vaca, and Alcides López-Milla. "Synthesis of Colloidal Gold Nanorods with Plasmon Absorbance Wavelength in the Near Infrared Region." MRS Advances 1, no. 30 (2016): 2181–86. http://dx.doi.org/10.1557/adv.2016.486.

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ABSTRACTWe report preparation and characterization of colloidal gold nanorods (AuNR) with plasmon absorbance over 950 nm and an aspect ratio of 7.2 by tuning silver nitrate concentration and the CTAB/BDAC ratio during growth. Samples were analyzed by scanning transmission electron microscopy, X-ray diffraction, UV-vis absorption spectroscopy and measurement of zeta potential. Addition of 170 μM silver nitrate to the CTAB-containing reaction mixture allowed preparation of AuNRs with a maximum absorbance peak at 941.8 nm and a yield around 96%. Optimization of the BDAC/CTAB ratio allowed for a f
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Mohamed, Ashraf A. "Kinetic and maximum-absorbance spectrophotometric methods for the determination of olanzapine." Monatshefte für Chemie - Chemical Monthly 139, no. 9 (2008): 1005–10. http://dx.doi.org/10.1007/s00706-008-0894-4.

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AL-Jalali, Muhammad Ahmad. "P, Q, and R branches of the pure oxygen A-band at 762 nm." International Journal of Physical Research 13, no. 1 (2025): 12–22. https://doi.org/10.14419/h3rchs22.

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Oxygen A-band absorption spectra exhibit interference between O2 monomer (with a discrete line at 762 nm) and O2 dimol (which ‎also exhibits a band spectrum at 762 nm) absorption. Analysis of the absorbance spectrum between 750 nm and 770 nm reveals ‎P, Q, and R branches, characteristic of rovibrational transitions. Pure oxygen spectra were analyzed at pressures ranging from 1 to 25 bar and at temperatures of 298 K, 323 K, 348 K, and 373 K using advanced data analysis methods. Integrated absorbance, Gaussian and ‎Lorentzian full width at half maximum height (FWHM), and maximum absorbance peak
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Book chapters on the topic "Maximum absorbance"

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ÁVILA-MARES, Isidro Mildred, Fidel Benjamín ALARCÓN-HERNÁNDEZ, María del Carmen FUENTES-ALBARRÁN, and José Luis GADEA-PACHECO. "The behavior of the pigment discoloration Permalon Rhodamine B 400% red in a liquid medium after exposure to a non-thermal plasma of different electrical power at atmospheric pressure." In Engineering and Applied Sciences. ECORFAN, 2023. http://dx.doi.org/10.35429/h.2023.6.50.59.

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The behavior of the discoloration of the pigment Permalon Rhodamine B 400% red in a liquid medium (120 mg/L) derived from exposure to a non-thermal air plasma at atmospheric pressure was quantified experimentally. Observed changes in dilution were measured as a function of plasma generation electrical power (40.0 W, 60.0 W, 80.0 W, and 100.0 W), as well as exposure time (150 minutes per power). Solution-generated effects were monitored by UV/VIS spectrophotometry measurements (absorbance; λ = 533 nm), pH, and temperature change. The absorbance of the solution increased at the beginning of the
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Tu, Changqing, and Xinrong Wen. "Spectrophotometric Determination of Cysteine Hydrochloride Content by Ferric Chloride-Potassium Ferricyanide." In Studies in Health Technology and Informatics. IOS Press, 2023. http://dx.doi.org/10.3233/shti230839.

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In the acidic medium, hydrosulfuryl(-SH) in cysteine hydrochloride can reduce Fe3+ to Fe2+, then Fe2+ react with potassium ferricyanide to form KFe[Fe(CN)6](soluble Prussian blue). Prussian blue has a maximum absorption at 727 nm, Bill’s law is observed between mass concentration of cysteine hydrochloride and absorbance of Prussian blue, the content of cysteine hydrochloride is indirectly determinated by measuring the absorbance of Prussian blue. An accurate, simple, fast spectrophotometric method for the determination of cysteine hydrochloride content by ferric chloride-potassium ferricyanide
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Swathi, K., and Sanganabattla Supraja. "Isolation, Production, Extraction, Optimization and Fortification of PHB using Silver Nanoparticles from Lactobacillus Casei." In Convergence of Technology & Biology - Transforming Life Sciences. Shanlax Publications, 2025. https://doi.org/10.34293/ctbtls.2025.ch005.

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In this study, bacteria were first isolated to produce the biopolymer PHB (poly - 3 hydroxybutyrate) as a substitute for plastic, which is a major contributor to soil pollution and excess plastic wastage worldwide. Plastic lacks the property to degrade in soil but is used in large forms. To overcome this problem, bioplastics are synthesized which are biodegradable compounds that are completely sustainable and can substitute fossil fuels and non-degradable plastic. In this study, the ideal organism for the production of PHB was first isolated from soil samples and then grown on suitable media.
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Gopal, N. Madana, L. Siva Sanker Reddy, and R. Nageswara Rao. "METHOD DEVELOPMENT AND VALIDATION OF CILNIDIPINE IN TABLET DOSAGE FORM BY USING ULTRA VIOLET SPECTROPHOTOMETRY." In Futuristic Trends in Pharmacy & Nursing Volume 3 Book 11. Iterative International Publisher, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bgpn11p2ch5.

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A simple, quick, and accurate UV Spectroscopic method was developed to quantify celecoxib in API and tablet formulations. Celecoxib was used to treat rheumatoid arthritis and osteoarthritis. The proposed procedure was developed using acetonitrile and distilled water as the solvents in a 50:50 volume-to-volume ratio, and it was optimized using a Shimadzu UV-1800 ENG240V at a maximum wavelength of 240 nm with an absorbance of 0.558. The sensitivity, robustness, accuracy, and precision of the improved technique Degradation studies were also conducted and determined to be within the constraints. T
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de Oliveira, Vitória Rocha, and Fred Augusto Ribeiro Nogueira. "Spectrophotometric investigation of natural dyes of Portulaca grandiflora and Catharanthus roseus flowers." In Communication and Culture: Multidisciplinary Perspectives. Seven Editora, 2024. http://dx.doi.org/10.56238/sevened2024.014-023.

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The study of dyes of native flowers contributes to a better investigation of these species, which can motivate new economic activities in the country. The objective of this work was to characterize by spectrophotometry the natural dyes extracted from the petals of the flowers Portulaca grandiflora and Catharanthus roseus. The extraction of the dyes was carried out by maceration of the petals of the dried flowers and under different experimental conditions to evaluate the extraction time and the type of solvent used. The dyes obtained were characterized by spectrophotometry in the UV/Visible re
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B. Patil, Ashokrao, and Satish K. Pardeshi. "ZINC OXIDE MEDIATED SOLAR PHOTOCATALYSIS." In Futuristic Trends in Chemical Material Sciences & Nano Technology Volume 3 Book 7. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3becs7p8ch1.

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Zinc oxide photo catalyst samples were synthesized by co-precipitation, solid state reaction and mechano chemical method. The photo catalysts thus prepared were characterized by various techniques such as powder XRD, SEM, EDX, XPS, TG-DTA, FT-IR, PL and UV-visible spectrophotometer. The solar photo catalytic activity of these semiconductors was examined by means of degradation of hazardous and endocrine disrupting chemicals such as Phenol, Resorcinol, and Bisphenol A in batch photo reactor. The effect of various parameters such as calcination temperature, amount of photo catalyst, concentratio
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Santos, Renata Cristina Dos, Raphaela Ribas Verbinenn, Mariana Camargo Lourenço, Tania Maria Bordin Bonfim, Thais Martins Guimarães, and Débora Brand. "CASSAVA RESIDUES AS LOW-COST MEDIA FOR THE PRODUCTION OF MONASCUS PURPUREUS PIGMENTS BY SOLID STATE FERMENATION." In Open Science Research XII. Editora Científica Digital, 2023. http://dx.doi.org/10.37885/230613517.

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Objective: The objective of this work was the production of red and yellow pigments by the fungus Monascus purpureus NRRL 1596 using agroindustrial wastes of the cassava production chain. Methods: Solid state fermentation experiments were carried out by using cassava peels and cassava wastewater as substrate for pigment production by Monascus purpureus NRRL 1596. Initial moisture content and the addition of soy bran to improve Carbon/nitrogen ratio were optimized in order to follow the growth kinetics and pigment production in the selected substrate. Total sugars, biomass, pigments, pH and moi
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Shoda, Makoto. "Application of Dec 1 for Decolorization of Other Colored Substances." In Decolorization by Thanatephorus Cucumeris Dec 1. BENTHAM SCIENCE PUBLISHERS, 2024. http://dx.doi.org/10.2174/9789815256895124010007.

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Molasses is a primary carbon source, especially in the microbial industry; however, molasses includes many colored substances, like melanoidins, which become concentrated by the Maillard reaction after sterilization [41]. Thus, these remain in the Molasses Wastewater (MWW) after use. For effective treatment of MWW, biological methods are attracting attention. Section 1-10, in the previous Chapter 3, showed molasses to be available as a carbon source for the growth of Dec 1, and that partial color removal of molasses by Dec 1 was possible. The enhanced color removal of molasses by Dec 1 was con
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"where K = kelvin. Because of the low temperature elevation in the low dose range, radiation calorimetry is limited in practice to the dose range above 3 kGy. This small temperature elevation is the gross result of the complex process of radiation interaction with matter. The individual steps of this process depend on the type of radiation used. Another type of physical dose meter, one that is used more and more in research and in industrial practice, is the alanine/electron spin resonance (ESR) system. Stable free radicals produced by irradiation in a concentration propor­ tional to the radiation dose in samples of pure, dry alanine are measured by ESR spectroscopy. The alanine is usually mixed 4:1 with paraffin (26) or 1:1 with polystyrene (27) of analytical grade quality. Reproducible dose response curves are obtained in the extremely wide dose range of 1 Gy to 100 kGy. In principal, any reproducible change caused by irradiation of a medium can be used to measure the absorbed radiation dose. In practice, only those changes can be evaluated which are stable for a reasonable length of time and which can be reliably measured by standard procedures such as titration or spectrophotometry. The chemical change is usually expressed as the G value, which is a measure of the number of atoms, molecules, or ions produced ( + G) or destroyed ( -G ) by 100 eV of absorbed energy. In the new SI system of units the G value is expressed as per J instead of per 100 eV. An important reference dose meter in food irradiation is the ferrous sulfate or Fricke dose meter. It is based on the radiation-induced oxidation of ferrous ions (Fe + ) to ferric ions (Fe + ) and consists of measuring the increased optical absorbance of the ferric ions at the absorption peak of 305 nm. For 60Co gamma rays the G value for ferric ion yield is 15.6 Fe3+ ions per 100 eV, or 9.74 X 1017 ions/J; the yield for electrons at a dose rate of 108 Gy/sec is 13.0. Fricke dosimetry is useful in the range 3 Gy. The upper limit can be extended into the kGy range by adding CuS04, which reduces the G value from 15.6 to 0.65. There are many other systems, such as the ethanol-chlorobenzene dose meter, which is based on the formation of hydrochloric acid from chlorobenzene. The hydrochloric acid can be measured by titration or by its effect on the dielectric constant. The useful dose range of this system is 1-400 Gy. In the low dose range, down to 5 Gy, radiochromic dye dosimetry can be used. When the colorless solution of pararosaniline cyanide in 2-methoxyethanol and glacial acetic acid is irradiated, an intense red color develops with an absorption maximum at 549 nm. More recently proposed methods belonging to the group of liquid dose meter systems are listed in Table 3. PMA (polymethyl methacrylate) dose meters belong to the group of solid phase dose meters. Irradiation of PMMA (e.g., Perspex) induces an absorption." In Safety of Irradiated Foods. CRC Press, 1995. http://dx.doi.org/10.1201/9781482273168-39.

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Conference papers on the topic "Maximum absorbance"

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Pan, Jhuma, and Sachin Kumar Srivastava. "Simulation of a Near Infrared Perfect Absorber Based on Plasmonic Meta-gratings." In Frontiers in Optics. Optica Publishing Group, 2022. http://dx.doi.org/10.1364/fio.2022.jtu4b.41.

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A perfect plasmonic meta-grating absorber in near infrared region was simulated with maximum absorbance of 99.57% and mimimum reflectance of 0 .36% a t 1795 nm. The high absorbance and lost cost fabrication makes this device a robust candidate for applications in smart windows and photodetectors.
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Verma, Sagar Kumar, and Sachin Kumar Srivastava. "Experimental Demonstration of Fano Resonance Based One Dimensional Plasmonic Metasurface Absorber for Refractive Index Sensing in NIR Regime." In Frontiers in Optics. Optica Publishing Group, 2023. http://dx.doi.org/10.1364/fio.2023.jw4a.45.

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A Fano resonance-based one-dimensional plasmonic metasurface absorber (PMA) has been fabricated for the NIR regime with a maximum absorbance of 60% at 800 angle of incidence. PMA could achieve a spectral-sensitivity of 3079.945 nm/RIU.
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Kubiak, G. D., E. M. Kneedler, K. W. Berger, J. E. Bjorkholm, W. M. Mansfield, and H. Windischmann. "Resist Characterization at Soft X-Ray Wavelengths." In Soft X-Ray Projection Lithography. Optica Publishing Group, 1991. http://dx.doi.org/10.1364/sxray.1991.thd3.

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The characterization of resist materials for soft X-ray projection lithography (SXPL) in the 75-350 Å spectral region is an important issue, although it has received little attention to date. With the recent demonstration of diffraction-limited SXPL at 140 Å[1], the need to optimize resists at specific exposure wavelengths for future SXPL commercialization has become acute. Near 300 Å resist absorbance is so large that a single-layer resist strategy does not appear feasible, whereas near 80 Å it does. At intermediate wavelengths, for example where Mo/Si multilayer reflective coatings are effic
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Kovačević, Marija, Marija Simić, Katarina Stojanović, et al. "PHOTOCATALYTIC DEGRADATION OF CIPROFLOXACIN WITH IRON- MODIFIED TITAN(IV) OXIDE." In 17th International Conference on Fundamental and Applied Aspects of Physical Chemistry. Society of Physical Chemists of Serbia, 2024. https://doi.org/10.46793/phys.chem24i.073k.

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Considering the increasing significance of emerging pollutants in the environment, this research investigates the photodegradation of ciprofloxacin, which ranks among the top three most detected antibiotics in aquatic ecosystems. Therefore, we synthesized TiO2 modified with Fe in three different molar ratios (0.5, 1 and 2 %) and applied it to the photodegradation of the ciprofloxacin infusion solution. The photodegradation process was monitored by UV-Vis spectroscopy for over 240 minutes. The results have shown that the efficiency rate is around 50 % for all three photocatalysts. However, the
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Pryor, Owen M., Erik Ninnemann, and Subith Vasu. "CO Time-Histories During Oxy-Methane Combustion in a CO2 Environment." In ASME Turbo Expo 2019: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/gt2019-92086.

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Abstract Carbon monoxide time-histories and ignition delay times were measured in carbon dioxide diluted methane mixtures behind reflected shockwaves. Experiments were performed around 2 atm for a temperature range between 1650–2000 K. The experiments were performed for a mixture of XCH4 = 0.5%, XO2 = 1.0%, XCO2 = 8.5%, XAr = 90.0%. The mixture was chosen to minimize energy release during the experiment and a minimum of 2 ms was recorded for all experiments. The carbon monoxide time-histories were measured using a tunable diode laser absorption spectroscopy technique and measuring the absorban
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Spry, Robert J., and David J. Kosan. "Theoretical analysis of the crystalline colloidal array filter." In OSA Annual Meeting. Optica Publishing Group, 1986. http://dx.doi.org/10.1364/oam.1986.mn4.

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The colloidal array filter consists of an aqueous suspension of polystyrene spheres arranged in a crystalline lattice, thereby producing Bragg diffraction of incident light. Recent measurements have shown that a rejection filter incorporating a thin layer of the colloidal suspension has a moderately narrow bandwidth and unusually large peak absorbance.1 We have successfully explained these results using both dynamic x-ray diffraction theory and optical scattering theory. The theoretical bandwidth is in good agreement with the experimental value, while the theoretical attenuation results are co
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Kandrac, Morgan, and Karen Schaich. "Epoxides are major products in oxidation of methyl oleate and linoleate and their triacylglycerols." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/wbbv6226.

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Lipid autoxidation poses a significant problem for stabilizing sensory quality, nutritional value, and chemical safety of lipid containing foods. Peroxide value and volatile carbonyls are the most measured markers to assess oxidation, but products such as epoxides and alcohols are now being identified and quantified in foods. The mechanisms and conditions under which other products form are poorly understood, particularly how lipid structure and oxidation conditions affect reaction pathways, products, and rates. This research paper aims to show how number of double bonds affects oxidation prod
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Zdravković, Milica, Vesna Grekulović, Edina Huseinović, et al. "Uv-vis identification of copper complexes with inhibitor organic components in the electrolyte." In Proceedings of XVI International Mineral Processing and Recycling Conference, Belgrade, 28-30.05.2025. University of Belgrade, Technical Faculty, Bor, 2024. https://doi.org/10.5937/imprc25226z.

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The UV-VIS method is an effective, non-destructive method that enables a better understanding of the electrolyte during corrosion. The aim of the study presented in this paper is to determine the existence of an organometallic complex in a 0.5 M NaCl solution with the addition of Rubus fruticosus L. leaf extract (RFLE). The experiments were conducted in a 0.5 M NaCl solution with the addition of RFLE (5 g/L and 10 g/L) with and without the immersion of a copper coupon. The results show that after the copper coupon standing in the electrolyte, there is a change in the absorbance maximum in the
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Korayem, Abd-Elrahman, Hamid Mohammadi, and Roozbeh Dargazany. "Experimental Investigation on the Performance Loss of Cross-Linked Polymers During Photo-Oxidative Aging." In ASME 2020 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/imece2020-24318.

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Abstract Polymeric substances have seen an unprecedented integration in countless applications and fields of life, subjecting them to a broad range of environmental conditions, which can cause a form of damage to the structural and mechanical properties of these components known as aging. In this study we investigate the effects of the degradation of polymeric materials due to the extended exposure to sunlight, which can be simulated through controlled photo-oxidative aging using ultraviolet (UV) radiation as the main catalyst of this phenomenon. The effects on mechanical behavior and chemical
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Hashemi, Nastaran. "Optofluidic Cytometry on a Chip." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80538.

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The effects of global warming, pollution in river effluents, and changing ocean currents can be studied by characterizing variations in phytoplankton populations. We demonstrate the design and fabrication of a microflow cytometer for characterization of phytoplankton. Guided by chevron-shaped grooves on the top and bottom of a microfluidic channel, two symmetric sheath streams wrap around a central sample stream and hydrodynamically focus it in the center of the channel. The lasers are carefully chosen to provide excitation light close to the maximum absorbance wavelengths for the intrinsic fl
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