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Journal articles on the topic 'MCD EPOS'

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Published: 4 June 2021
Last updated: 8 February 2022

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1

Heberlein, C., K. D. Fischer, M. Stoffel, et al. "The gene for erythropoietin receptor is expressed in multipotential hematopoietic and embryonal stem cells: evidence for differentiation stage-specific regulation." Molecular and Cellular Biology 12, no. 4 (1992): 1815–26. http://dx.doi.org/10.1128/mcb.12.4.1815.

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The principal regulator of erythropoiesis is the glycoprotein erythropoietin, which interacts with a specific cell surface receptor (EpoR). A study aimed at analyzing EpoR gene regulation has shown that both pluripotent embryonal stem cells and early multipotent hematopoietic cells express EpoR transcripts. Commitment to nonerythroid lineages (e.g., macrophage or lymphocytic) results in the shutdown of EpoR gene expression, whereas commitment to the erythroid lineage is concurrent with or followed by dramatic increases in EpoR transcription. To determine whether gene activity could be correlat
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2

Yoshimura, A., and H. F. Lodish. "In vitro phosphorylation of the erythropoietin receptor and an associated protein, pp130." Molecular and Cellular Biology 12, no. 2 (1992): 706–15. http://dx.doi.org/10.1128/mcb.12.2.706.

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The cytoplasmic domain of the cloned erythropoietin (EPO) receptor (EPOR) contains no protein kinase motif, yet addition of EPO to EPO-responsive cells causes an increase in protein-tyrosine phosphorylation. Here we show that addition of EPO or interleukin-3 (IL-3) to an IL-3-dependent cell line expressing the wild-type EPOR causes a small fraction (less than 5%) of total cellular EPOR to shift in gel mobility from 66 to 72 kDa, due at least in part to phosphorylation. Using biotinylated EPO as an affinity reagent, we show that the 72-kDa species is greatly enriched on the cell surface. To dem
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3

Hino, M., A. Tojo, Y. Misawa, H. Morii, F. Takaku, and M. Shibuya. "Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line." Molecular and Cellular Biology 11, no. 11 (1991): 5527–33. http://dx.doi.org/10.1128/mcb.11.11.5527.

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A murine erythroleukemia (MEL) cell line, F5-5, expressed 10,000 binding sites for erythropoietin (EPO) per cell, 10-fold more than was expressed by other murine erythroleukemia cell lines and normal erythroid progenitors. Northern (RNA) and Southern blot analyses revealed overexpression of mRNA for the EPO receptor (EPOR) and rearrangement of one of the EPOR gene alleles in F5-5 cells, respectively. Molecular cloning of F5-5-derived cDNA encoding EPOR revealed that the 5' noncoding region of the EPOR cDNA corresponds to the 3' long terminal repeat sequence of the polycythemic strain of Friend
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4

Youssoufian, H., and H. F. Lodish. "Transcriptional inhibition of the murine erythropoietin receptor gene by an upstream repetitive element." Molecular and Cellular Biology 13, no. 1 (1993): 98–104. http://dx.doi.org/10.1128/mcb.13.1.98.

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Transcription of the murine erythropoietin receptor (EpoR) gene is inhibited by a novel repetitive element that is located upstream of the EpoR promoter. Reporter gene studies reveal that the inhibitory effect is both distance and orientation dependent. This element is a member of a family of repetitive elements specific to rodents and is present at approximately 10(5) copies per mouse genome. It encodes approximately 500- to 900-bp-long transcripts in both erythroid and nonerythroid cells. RNase protection analysis with a probe from the 5' flanking murine EpoR gene reveals that the direction
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5

Heberlein, C., K. D. Fischer, M. Stoffel, et al. "The gene for erythropoietin receptor is expressed in multipotential hematopoietic and embryonal stem cells: evidence for differentiation stage-specific regulation." Molecular and Cellular Biology 12, no. 4 (1992): 1815–26. http://dx.doi.org/10.1128/mcb.12.4.1815-1826.1992.

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The principal regulator of erythropoiesis is the glycoprotein erythropoietin, which interacts with a specific cell surface receptor (EpoR). A study aimed at analyzing EpoR gene regulation has shown that both pluripotent embryonal stem cells and early multipotent hematopoietic cells express EpoR transcripts. Commitment to nonerythroid lineages (e.g., macrophage or lymphocytic) results in the shutdown of EpoR gene expression, whereas commitment to the erythroid lineage is concurrent with or followed by dramatic increases in EpoR transcription. To determine whether gene activity could be correlat
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6

Yoshimura, A., and H. F. Lodish. "In vitro phosphorylation of the erythropoietin receptor and an associated protein, pp130." Molecular and Cellular Biology 12, no. 2 (1992): 706–15. http://dx.doi.org/10.1128/mcb.12.2.706-715.1992.

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The cytoplasmic domain of the cloned erythropoietin (EPO) receptor (EPOR) contains no protein kinase motif, yet addition of EPO to EPO-responsive cells causes an increase in protein-tyrosine phosphorylation. Here we show that addition of EPO or interleukin-3 (IL-3) to an IL-3-dependent cell line expressing the wild-type EPOR causes a small fraction (less than 5%) of total cellular EPOR to shift in gel mobility from 66 to 72 kDa, due at least in part to phosphorylation. Using biotinylated EPO as an affinity reagent, we show that the 72-kDa species is greatly enriched on the cell surface. To dem
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7

Yamamura, Yasuko, Hisato Senda, Yukio Kageyama, Tomoko Matsuzaki, Makoto Noda, and Yoji Ikawa. "Erythropoietin and Friend Virus gp55 Activate Different JAK/STAT Pathways through the Erythropoietin Receptor in Erythroid Cells." Molecular and Cellular Biology 18, no. 3 (1998): 1172–80. http://dx.doi.org/10.1128/mcb.18.3.1172.

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ABSTRACT Abnormal erythropoietin (EPO)-independent cell growth is induced after infection of erythroid progenitor cells with a polycythemic strain of Friend virus (FVp). Binding of its Env-related glycoprotein (gp55) to the EPO receptor (EPOR) mimics the activation of the EPOR with EPO. We investigated the gp55-EPOR signaling in erythroblastoid cells from mice infected with FVp and in cells of FVp-induced or gp55-transgenic-mouse-derived erythroleukemia cell lines, comparing it with the EPO-EPOR signaling in EPO-responsive erythroblastoid cells. While the Janus protein tyrosine kinase JAK2 and
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8

Hino, M., A. Tojo, Y. Misawa, H. Morii, F. Takaku, and M. Shibuya. "Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line." Molecular and Cellular Biology 11, no. 11 (1991): 5527–33. http://dx.doi.org/10.1128/mcb.11.11.5527-5533.1991.

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A murine erythroleukemia (MEL) cell line, F5-5, expressed 10,000 binding sites for erythropoietin (EPO) per cell, 10-fold more than was expressed by other murine erythroleukemia cell lines and normal erythroid progenitors. Northern (RNA) and Southern blot analyses revealed overexpression of mRNA for the EPO receptor (EPOR) and rearrangement of one of the EPOR gene alleles in F5-5 cells, respectively. Molecular cloning of F5-5-derived cDNA encoding EPOR revealed that the 5' noncoding region of the EPOR cDNA corresponds to the 3' long terminal repeat sequence of the polycythemic strain of Friend
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9

Youssoufian, H., and H. F. Lodish. "Transcriptional inhibition of the murine erythropoietin receptor gene by an upstream repetitive element." Molecular and Cellular Biology 13, no. 1 (1993): 98–104. http://dx.doi.org/10.1128/mcb.13.1.98-104.1993.

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Abstract:
Transcription of the murine erythropoietin receptor (EpoR) gene is inhibited by a novel repetitive element that is located upstream of the EpoR promoter. Reporter gene studies reveal that the inhibitory effect is both distance and orientation dependent. This element is a member of a family of repetitive elements specific to rodents and is present at approximately 10(5) copies per mouse genome. It encodes approximately 500- to 900-bp-long transcripts in both erythroid and nonerythroid cells. RNase protection analysis with a probe from the 5' flanking murine EpoR gene reveals that the direction
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10

Pelletier, Stéphane, Sébastien Gingras, Megumi Funakoshi-Tago, Sherié Howell, and James N. Ihle. "Two Domains of the Erythropoietin Receptor Are Sufficient for Jak2 Binding/Activation and Function." Molecular and Cellular Biology 26, no. 22 (2006): 8527–38. http://dx.doi.org/10.1128/mcb.01035-06.

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ABSTRACT Biochemical and genetic studies have shown that Jak2 is an essential component of EpoR signal transduction which is required for normal erythropoiesis. However, whether Jak2 is the sole direct mediator of EpoR signal transduction remains controversial. To address this issue, we have used an extensive and systematic mutational analysis across the EpoR cytoplasmic tail and transmembrane domain with the goal of determining whether mutants that negatively affected EpoR biological activity but retained Jak2 activation could be identified. Analysis of over 40 mutant receptors established th
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11

Casarrubios, Laura, Alberto Polo-Montalvo, María Concepción Serrano, et al. "Effects of Ipriflavone-Loaded Mesoporous Nanospheres on the Differentiation of Endothelial Progenitor Cells and Their Modulation by Macrophages." Nanomaterials 11, no. 5 (2021): 1102. http://dx.doi.org/10.3390/nano11051102.

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Angiogenic biomaterials are designed to promote vascularization and tissue regeneration. Nanoparticles of bioactive materials loaded with drugs represent an interesting strategy to stimulate osteogenesis and angiogenesis and to inhibit bone resorption. In this work, porcine endothelial progenitor cells (EPCs), essential for blood vessel formation, were isolated and characterized to evaluate the in vitro effects of unloaded (NanoMBGs) and ipriflavone-loaded nanospheres (NanoMBG-IPs), which were designed to prevent osteoporosis. The expression of vascular endothelial growth factor receptor 2 (VE
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12

Miura, O., J. L. Cleveland, and J. N. Ihle. "Inactivation of erythropoietin receptor function by point mutations in a region having homology with other cytokine receptors." Molecular and Cellular Biology 13, no. 3 (1993): 1788–95. http://dx.doi.org/10.1128/mcb.13.3.1788.

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The cytoplasmic domain of the erythropoietin receptor (EpoR) contains a region, proximal to the transmembrane domain, that is essential for function and has homology with other members of the cytokine receptor family. To explore the functional significance of this region and to identify critical residues, we introduced several amino acid substitutions and examined their effects on erythropoietin-induced mitogenesis, tyrosine phosphorylation, and expression of immediate-early (c-fos, c-myc, and egr-1) and early (ornithine decarboxylase and T-cell receptor gamma) genes in interleukin-3-dependent
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13

Huang, Chi-Yo, Shih-Yu Chang, Yu-Hsien Yang, and Gwo-Hshiung Tzeng. "Next Generation Passive Optical Networking Technology Predictions by Using Hybrid MCDM Methods." Journal of Advanced Computational Intelligence and Intelligent Informatics 15, no. 4 (2011): 400–405. http://dx.doi.org/10.20965/jaciii.2011.p0400.

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The evolution of broadband telecommunication technologies has enabled applications over networks, which, in turn, has enabled the emergence of novel network services and applications such as VoIP and video on demand in triggering demand for higher network bandwidth. Passive Optical Networking (PON) is suitable for eliminating bandwidth insufficiency, so national governments and communities are observing the emergence of Fiber-To-The-Home (FTTH) technologies. Ethernet-based Passive Optical Networks (EPON) and Gigabit-based PON (GPON) are two candidates for next-generation PON in bandwidth effic
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14

Miura, O., A. D'Andrea, D. Kabat, and J. N. Ihle. "Induction of tyrosine phosphorylation by the erythropoietin receptor correlates with mitogenesis." Molecular and Cellular Biology 11, no. 10 (1991): 4895–902. http://dx.doi.org/10.1128/mcb.11.10.4895.

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A role for tyrosine phosphorylation in the signal-transducing mechanisms of several hematopoietic growth factors has been hypothesized. To extend these observations, we have examined the effects of erythropoietin (Epo) on tyrosine phosphorylation in an Epo-responsive cell that was obtained by transfecting the murine erythropoietin receptor (EpoR) into an interleukin-3 (IL-3)-dependent cell line. By two-dimensional analysis of phosphotyrosine-containing proteins isolated with a monoclonal antibody (1G2) against phosphotyrosine, Epo and IL-3 were found to rapidly induce tyrosine phosphorylation
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15

Miura, O., J. L. Cleveland, and J. N. Ihle. "Inactivation of erythropoietin receptor function by point mutations in a region having homology with other cytokine receptors." Molecular and Cellular Biology 13, no. 3 (1993): 1788–95. http://dx.doi.org/10.1128/mcb.13.3.1788-1795.1993.

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Abstract:
The cytoplasmic domain of the erythropoietin receptor (EpoR) contains a region, proximal to the transmembrane domain, that is essential for function and has homology with other members of the cytokine receptor family. To explore the functional significance of this region and to identify critical residues, we introduced several amino acid substitutions and examined their effects on erythropoietin-induced mitogenesis, tyrosine phosphorylation, and expression of immediate-early (c-fos, c-myc, and egr-1) and early (ornithine decarboxylase and T-cell receptor gamma) genes in interleukin-3-dependent
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16

Miura, O., A. D'Andrea, D. Kabat, and J. N. Ihle. "Induction of tyrosine phosphorylation by the erythropoietin receptor correlates with mitogenesis." Molecular and Cellular Biology 11, no. 10 (1991): 4895–902. http://dx.doi.org/10.1128/mcb.11.10.4895-4902.1991.

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A role for tyrosine phosphorylation in the signal-transducing mechanisms of several hematopoietic growth factors has been hypothesized. To extend these observations, we have examined the effects of erythropoietin (Epo) on tyrosine phosphorylation in an Epo-responsive cell that was obtained by transfecting the murine erythropoietin receptor (EpoR) into an interleukin-3 (IL-3)-dependent cell line. By two-dimensional analysis of phosphotyrosine-containing proteins isolated with a monoclonal antibody (1G2) against phosphotyrosine, Epo and IL-3 were found to rapidly induce tyrosine phosphorylation
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17

Villamil Parra, Wilder Andrés, Adriana Del Pilar Acero, Francy Fabra, et al. "Métodos de medición de la capacidad aeróbica y la fuerza muscular en pacientes con enfermedad pulmonar obstructiva crónica en un ambiente intrahospitalario." Movimiento Científico 11, no. 2 (2018): 55–62. http://dx.doi.org/10.33881/2011-7191.mct.11202.

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Antecedentes: La enfermedad pulmonar obstructiva crónica (EPOC) es la deficiencia respiratoria de mayor prevalencia e impacto socioeconómico en el mundo. Por su alta incidencia, su curso clínico progresivo y sus requerimientos asistenciales constituyen un problema de salud pública de primer orden, es por ello que surge la necesidad de realizar una evaluación que determine el nivel de deterioro de las capacidades condicionales como fuerza y capacidad aeróbica. Objetivo: Identificar los métodos utilizados para la evaluación de la capacidad aeróbica y la fuerza muscular en pacientes con EPOC en u
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18

Guan, Xiumei, Hong Li, Xin Li, et al. "The Role of Autophagy in the Differentiation of EPCs Induced by Shear Stress." Molecular & Cellular Biomechanics 16, s1 (2019): 91. http://dx.doi.org/10.32604/mcb.2019.05755.

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19

Liu, Na, Xiaoyun Zhang, Yuzhen Ding, Hong Li, Xiumei Guan, and Min Cheng and Xiaodong Cui. "High Glucose Reduces the Shear Stress-Induced CD59 Expression on EPCs through F-Actin Alteration." Molecular & Cellular Biomechanics 16, s1 (2019): 87. http://dx.doi.org/10.32604/mcb.2019.05751.

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20

Gao, Yu, Meiyue Wang, Yanting He, Lanlan Li, Xiaodong Cui, and Min Cheng and Xiaoyun Zhang. "The Role of P53 in Transdifferentiation of EPCs into Smooth Muscle Cells Induced by Oscillatory Shear Stress." Molecular & Cellular Biomechanics 16, s1 (2019): 93. http://dx.doi.org/10.32604/mcb.2019.05758.

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21

Cohen-Cohen, Salomon, Giuseppe Lanzino, Waleed Brinjikji, et al. "Management of Mobile Thrombus With Distal Protection Devices During Carotid Artery Angioplasty: 2-Dimensional Operative Video." Operative Neurosurgery 21, no. 3 (2021): E242—E243. http://dx.doi.org/10.1093/ons/opab131.

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Abstract Embolic protection devices (EPDs) have become a standard of care during internal carotid artery revascularization.1,2 This video is about a 57-yr-old-male who presented with a wake-up stroke with a left hemispheric syndrome. Head computed tomography angiography (CTA) revealed tandem occlusions of the proximal left internal carotid artery (ICA) and of the distal left middle cerebral artery (MCA) with an ASPECT (Alberta Stroke Program Early CT Score) score of 6. The patient underwent a cerebral angiogram and was treated with balloon angioplasty with a distal EPD and mechanical thrombect
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22

Quelle, F. W., D. Wang, T. Nosaka, et al. "Erythropoietin induces activation of Stat5 through association with specific tyrosines on the receptor that are not required for a mitogenic response." Molecular and Cellular Biology 16, no. 4 (1996): 1622–31. http://dx.doi.org/10.1128/mcb.16.4.1622.

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The cytoplasmic domain of the erythropoietin receptor (EpoR) contains a membrane-distal region that is dispensable for mitogenesis but is required for the recruitment and tyrosine phosphorylation of a variety of signaling proteins. The membrane-proximal region of 96 amino acids is necessary and sufficient for mitogenesis as well as Jak2 activation, induction of c-fos, c-myc, cis, the T-cell receptor gamma locus (TCR-gamma), and c-pim-1. The studies presented here demonstrate that this region is also necessary and sufficient for the activation of Stat5A and Stat5B. The membrane-proximal domain
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23

Fischer, D. F., S. Gibbs, P. van De Putte, and C. Backendorf. "Interdependent transcription control elements regulate the expression of the SPRR2A gene during keratinocyte terminal differentiation." Molecular and Cellular Biology 16, no. 10 (1996): 5365–74. http://dx.doi.org/10.1128/mcb.16.10.5365.

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Expression of the SPRR2A gene, a member of the small proline-rich family of cornified cell envelope precursor proteins, is strictly linked to keratinocyte terminal differentiation both in vivo and in vitro. In this study, we explored the molecular mechanisms underlying this regulation in transiently transfected primary keratinocytes induced to differentiate in vitro. Deletion mapping and site-directed mutagenesis of SPRR2A promoter-chloramphenicol acetyltransferase constructs indicate that four transcription control elements are essential and sufficient for promoter activity. These elements we
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24

Funakoshi-Tago, Megumi, Stéphane Pelletier, Hiroshi Moritake, Evan Parganas, and James N. Ihle. "Jak2 FERM Domain Interaction with the Erythropoietin Receptor Regulates Jak2 Kinase Activity." Molecular and Cellular Biology 28, no. 5 (2007): 1792–801. http://dx.doi.org/10.1128/mcb.01447-07.

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ABSTRACT Janus kinases are essential for signal transduction by a variety of cytokine receptors and when inappropriately activated can cause hematopoietic disorders and oncogenesis. Consequently, it can be predicted that the interaction of the kinases with receptors and the events required for activation are highly controlled. In a screen to identify phosphorylation events regulating Jak2 activity in EpoR signaling, we identified a mutant (Jak2-Y613E) which has the property of being constitutively activated, as well as an inactivating mutation (Y766E). Although no evidence was obtained to indi
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25

Alfonso Mantilla, José Iván, Jaime Martínez Santa, and Olga Cecilia Vargas. "KINESIOGENÓMICA: UNA NUEVA PERSPECTIVA DE INVESTIGACIÓN EN FISIOTERAPIA." Movimiento Científico 10, no. 1 (2016): 78–86. http://dx.doi.org/10.33881/2011-7191.mct.10107.

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Introducción: el campo deportivo es un ámbito de investigación de diversas áreas de las ciencias de la salud, la kinesiogenómica realiza el estudio de secuencias genéticas comprometidas en el rendimiento deportivo, con la aparición del mapeo genético se ha abierto una brecha a la investigación de la genética como agente de interacción del potencial humano.Objetivo: realizar una revisión sistemática de la literatura sobre el campo de la genética aplicada en el área deportiva y clínica.Materiales y métodos: se realizó una revisión sistemática en las siguientes bases de datos: “Ebsco” “Pedro” “Hi
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26

Betancourt Peña, Johnatan, Astrid Carolina Mosquera García, Leidy Marcela Orozco Henao, et al. "Caracterización de pacientes con enfermedad pulmonar obstructiva crónica que inician un programa de rehabilitación pulmonar." Movimiento Científico 11, no. 2 (2018): 47–54. http://dx.doi.org/10.33881/2011-7191.mct.11201.

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Objetivo: describir las características de ingreso en pacientes con enfermedad pulmonar obstructiva crónica que ingresan a un programa de rehabilitación pulmonar en una IPS de la ciudad de Cali. Método: estudio observacional de tipo transversal. Se recolectó información sobre las características sociodemográficos, clínicas y capacidad funcional de 130 pacientes con Enfermedad Pulmonar Obstructiva Crónica en el periodo de julio de 2012 a junio de 2014, en un programa de rehabilitación pulmonar de una institución prestadora de servicios de salud en la ciudad de Cali, Colombia. Resultados: la eda
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27

Kosaka, Nobuyoshi, Yusuke Yamamoto, Keiichi Sugiura, et al. "Regulation of miR-210 Generation in Response to Hypoxia in Erythrocytic Cells." Blood 110, no. 11 (2007): 1247. http://dx.doi.org/10.1182/blood.v110.11.1247.1247.

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Abstract EPO-EPOR-mediated intracellular signaling activates JAK/STAT, ras/raf/MAP kinase and PI3 kinase/Akt cascades to promote cell survival, proliferation and differentiation. Alternatively, recent studies suggest that microRNAs (miRNAs) are a novel class of small noncoding RNAs that regulate gene expression at the post-transcriptional level and play a critical role in many important biological processes. Although recent reports showed the importance of miRNA functions in hematopoietic cells, the effect of EPO on miRNA expression and the role of miRNA in modulating EPO-mediated cell prolife
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28

"MCD EPOS intelligent compact drives from maxon motor." Assembly Automation 28, no. 2 (2008). http://dx.doi.org/10.1108/aa.2008.03328bad.006.

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29

Zhong, Qiang, and Qingling Yang. "Analyzing the Mental States of the Sports Student Based on Augmentative Communication with Human-Computer Interaction." Journal of Interconnection Networks, July 16, 2021, 2141006. http://dx.doi.org/10.1142/s0219265921410061.

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A system is proposed for mental condition assessment (MCA) system in adaptive testing complexes. The complexity involves the Emotiv Epoc+ neuroheadset with fourteen channels of the EEG digital network and the ECG-recorder for collecting signals for cardiac output (HRV). In various processing States, biochemical values were recorded in Ten healthy individuals: history (available remainder), TOVA-test (psychological stack), hypotension (physiological load), and after cutting. The somewhat reduced HRV continuum’s strength was observed to change dramatically, induced by a supremacy-segmental heart
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30

Chen, Weitao, Shenhai Ran, Canhui Wu, and Bengt Jacobson. "Explicit parallel co-simulation approach: analysis and improved coupling method based on H-infinity synthesis." Multibody System Dynamics, March 10, 2021. http://dx.doi.org/10.1007/s11044-021-09785-x.

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AbstractCo-simulation is widely used in the industry for the simulation of multidomain systems. Because the coupling variables cannot be communicated continuously, the co-simulation results can be unstable and inaccurate, especially when an explicit parallel approach is applied. To address this issue, new coupling methods to improve the stability and accuracy have been developed in recent years. However, the assessment of their performance is sometimes not straightforward or is even impossible owing to the case-dependent effect. The selection of the coupling method and its tuning cannot be per
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31

Young, Sherman. "Beyond the Flickering Screen: Re-situating e-books." M/C Journal 11, no. 4 (2008). http://dx.doi.org/10.5204/mcj.61.

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The move from analog distribution to online digital delivery is common in the contemporary mediascape. Music is in the midst of an ipod driven paradigm shift (Levy), television and movie delivery is being reconfigured (Johnson), and newspaper and magazines are confronting the reality of the world wide web and what it means for business models and ideas of journalism (Beecher). In the midst of this change, the book publishing industry remains defiant. While embracing digital production technologies, the vast majority of book content is still delivered in material form, printed and shipped the o
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