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Journal articles on the topic 'MCLEAs'

Author: Grafiati

Published: 10 September 2021

Last updated: 17 July 2025

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1

Ifko, Dušica, Katja Vasić, Željko Knez, and Maja Leitgeb. "(Magnetic) Cross-Linked Enzyme Aggregates of Cellulase from T. reesei: A Stable and Efficient Biocatalyst." Molecules 28, no. 3 (2023): 1305. http://dx.doi.org/10.3390/molecules28031305.

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Cross-linked enzyme aggregates (CLEAs) represent an effective tool for carrier-free immobilization of enzymes. The present study promotes a successful application of functionalized magnetic nanoparticles (MNPs) for stabilization of cellulase CLEAs. Catalytically active CLEAs and magnetic cross-linked enzyme aggregates (mCLEAs) of cellulase from Trichoderma reesei were prepared using glutaraldehyde (GA) as a cross-linking agent and the catalytic activity and stability of the CLEAs/mCLEAs were investigated. The influence of precipitation agents, cross-linker concentration, concentration of enzyme, addition of bovine serum albumin (BSA), and addition of sodium cyanoborohydride (NaBH3CN) on expressed activity and immobilization yield of CLEAs/mCLEAs was studied. Particularly, reducing the unsaturated Schiff’s base to form irreversible linkages is important and improved the activity of CLEAs (86%) and mCLEAs (91%). For increased applicability of CLEAs/mCLEAs, we enhanced the activity and stability at mild biochemical process conditions. The reusability after 10 cycles of both CLEAs and mCLEAs was investigated, which retained 72% and 65% of the initial activity, respectively. The thermal stability of CLEAs and mCLEAs in comparison with the non-immobilized enzyme was obtained at 30 °C (145.65% and 188.7%, respectively) and 50 °C (185.1% and 141.4%, respectively). Kinetic parameters were determined for CLEAs and mCLEAs, and the KM constant was found at 0.055 ± 0.0102 mM and 0.037 ± 0.0012 mM, respectively. The maximum velocity rate (Vmax) was calculated as 1.12 ± 0.0012 µmol/min for CLEA and 1.17 ± 0.0023 µmol/min for mCLEA. Structural characterization was studied using XRD, SEM, and FT-IR. Catalytical properties of immobilized enzyme were improved with the addition of reducent NaBH3CN by enhancing the activity of CLEAs and with addition of functionalized aminosilane MNPs by enhancing the activity of mCLEAs.

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2

de Freitas, Lucas A., Marylane de Sousa, Laiza B. Ribeiro, Ítalo W. L. de França та Luciana R. B. Gonçalves. "Magnetic CLEAs of β-Galactosidase from Aspergillus oryzae as a Potential Biocatalyst to Produce Tagatose from Lactose". Catalysts 13, № 2 (2023): 306. http://dx.doi.org/10.3390/catal13020306.

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β-galactosidase is an enzyme capable of hydrolysing lactose, used in various branches of industry, mainly the food industry. As the efficient industrial use of enzymes depends on their reuse, it is necessary to find an effective method for immobilisation, maintaining high activity and stability. The present work proposes cross-linked magnetic cross-linked enzyme aggregates (mCLEAs) to prepare heterogeneous biocatalysts of β-galactosidase. Different concentrations of glutaraldehyde (0.6%, 1.0%, 1.5%), used as a cross-linking agent, were studied. The use of dextran-aldehyde as an alternative cross-linking agent was also evaluated. The mCLEAs presented increased recovered activity directly related to the concentration of glutaraldehyde. Modifications to the protocol to prepare mCLEAs with glutaraldehyde, adding a competitive inhibitor or polymer coating, have not been effective in increasing the recovered activity of the heterogeneous biocatalysts or its thermal stability. The biocatalyst prepared using dextran-aldehyde presented 73.6% recovered activity, aside from substrate affinity equivalent to the free enzyme. The thermal stability at 60 °C was higher for the biocatalyst prepared with glutaraldehyde (mCLEA-GLU-1.5) than the one produced with dextran-aldehyde (mCLEA-DEX), and the opposite happened at 50 °C. Results obtained for lactose hydrolysis, the use of its product to produce a rare sugar (D-tagatose) and operational and storage stability indicate that heterogeneous biocatalysts have adequate characteristics for industrial use.

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Yang and Zhang. "Surfactant Imprinting Hyperactivated Immobilized Lipase as Efficient Biocatalyst for Biodiesel Production from Waste Cooking Oil." Catalysts 9, no. 11 (2019): 914. http://dx.doi.org/10.3390/catal9110914.

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Enzymatic production of biodiesel from waste cooking oil (WCO) could contribute to resolving the problems of energy demand and environment pollutions.In the present work, Burkholderia cepacia lipase (BCL) was activated by surfactant imprinting, and subsequently immobilized in magnetic cross-linked enzyme aggregates (mCLEAs) with hydroxyapatite coated magnetic nanoparticles (HAP-coated MNPs). The maximum hyperactivation of BCL mCLEAs was observed in the pretreatment of BCL with 0.1 mM Triton X-100. The optimized Triton-activated BCL mCLEAs was used as a highly active and robust biocatalyst for biodiesel production from WCO, exhibiting significant increase in biodiesel yield and tolerance to methanol. The results indicated that surfactant imprinting integrating mCLEAs could fix BCL in their active (open) form, experiencing a boost in activity and allowing biodiesel production performed in solvent without further addition of water. A maximal biodiesel yield of 98% was achieved under optimized conditions with molar ratio of methanol-to-WCO 7:1 in one-time addition in hexane at 40 °C. Therefore, the present study displays a versatile method for lipase immobilization and shows great practical latency in renewable biodiesel production.

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Chi, Meng-Chun, Yu-Fen Huang, Bo-Yuan Lu, Min-Guan Lin, Tzu-Fan Wang та Long-Liu Lin. "Magnetic Cross-Linked Enzyme Aggregates of a Transpeptidase-Specialized Variant (N450D) of Bacillus licheniformis γ-Glutamyl Transpeptidase: An Efficient and Stable Biocatalyst for l-Theanine Synthesis". Catalysts 11, № 2 (2021): 243. http://dx.doi.org/10.3390/catal11020243.

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γ-Glutamyl transpeptidase (GGT) catalyzes the transfer of glutathione’s γ-glutamyl group and related γ-glutamyl amides to water, amino acids or peptides, and utilizes a conserved Thr residue to process its own polypeptide chain into a large and a small subunit that then assemble to produce a catalytically competent enzyme. In this study, the magnetic cross-linked enzyme aggregates (mCLEAs) of a transpeptidase-specialized variant (N450D) of Bacillus licheniformis GGT were successfully prepared with optimized process parameters viz.1.25:1 (v/v) of isopropanol to N450D (0.3 mg/mL) ratio/0.02:1 (w/w) of enzyme to 3-aminopropyl triethoxysilane (APTES)-coated magnetic nanoparticle ratio/20 mM of glutaraldehyde. The prepared magnetic nanoparticles and immobilized enzyme (N450D-mCLEAs) were characterized by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy, field-emission scanning electron microscope integrated with energy dispersive X-ray spectroscopy (FESEM/EDS), and superparamagnetic analysis. As compared with free enzyme, N450D-mCLEAs displayed significantly higher heat resistance at temperatures of 55 and 60 °C, and had a greater stability over a storage period of one month. The immobilized enzyme could also be reused for 10 consecutive biocatalytic cycles with no significant reduction in the percent yield of l-theanine. Conclusively, this immobilization strategy surely provides a meaningful glance of developing N450D-mediated biocatalysis for the production of physiologically important γ-glutamyl compounds.

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5

Bellou, Myrto G., Michaela Patila, Renia Fotiadou, et al. "Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions." Biomolecules 13, no. 4 (2023): 643. http://dx.doi.org/10.3390/biom13040643.

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In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance. In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs). The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions. The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold. The biocatalyst retained the 100% of its initial activity after storage at −20 °C for 1 year and the 90% of its activity after 5 repeated cycles. Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES. The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.

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6

Lucena, Guilherme Nunes, Caio Carvalho dos Santos, Gabriel Cardoso Pinto, et al. "Magnetic cross-linked enzyme aggregates (MCLEAs) applied to biomass conversion." Journal of Solid State Chemistry 270 (February 2019): 58–70. http://dx.doi.org/10.1016/j.jssc.2018.11.003.

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7

Molina-Gutiérrez, María, Neumara Hakalin, Leonor Rodríguez-Sánchez, et al. "Effect of the Immobilization Strategy on the Efficiency and Recyclability of the Versatile Lipase from Ophiostoma piceae." Molecules 24, no. 7 (2019): 1313. http://dx.doi.org/10.3390/molecules24071313.

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The recombinant lipase from Ophiostoma piceae OPEr has demonstrated to have catalytic properties superior to those of many commercial enzymes. Enzymatic crudes with OPEr were immobilized onto magnetite nanoparticles by hydrophobicity (SiMAG-Octyl) and by two procedures that involve covalent attachment of the protein (mCLEAs and AMNP-GA), giving three nanobiocatalysts with different specific activity in hydrolysis of p-nitrophenyl butyrate (pNPB) and good storage stability at 4 °C over a period of 4 months. Free OPEr and the different nanobiocatalysts were compared for the synthesis of butyl esters of volatile fatty acids C4 to C7 in reactions containing the same lipase activity. The esterification yields and the reaction rates obtained with AMNP-GA-OPEr were in general higher or similar to those observed for the free enzyme, the mCLEAs-OPEr, and the non-covalent preparation SiMAG-Octyl-OPEr. The time course of the esterification of the acids C4 to C6 catalyzed by AMNP-GA-OPEr was comparable. The synthesis of the C7 ester was slower but very efficient, admitting concentrations of heptanoic acid up to 1 M. The best 1-butanol: acid molar ratio was 2:1 for all the acids tested. Depending on the substrate, this covalent preparation of OPEr maintained 80–96% activity over 7 cycles, revealing its excellent properties, easy recovery and recycling, and its potential to catalyze the green synthesis of chemicals of industrial interest.

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8

Kwon, Sena, Wanki Yoo, Young-Ok Kim, Kyeong Kyu Kim та T. Doohun Kim. "Molecular Characterization of a Novel Family VIII Esterase with β-Lactamase Activity (PsEstA) from Paenibacillus sp." Biomolecules 9, № 12 (2019): 786. http://dx.doi.org/10.3390/biom9120786.

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Molecular information about family VIII esterases, which have similarities with class C β-lactamases and penicillin-binding proteins, remains largely unknown. In this study, a novel family VIII esterase with β-lactamase activity (PsEstA) from Paenibacillus sp. was characterized using several biochemical and biophysical methods. PsEstA was effective on a broad range of substrates including tertiary butyl acetate, glyceryl tributyrate, glucose pentaacetate, olive oil, and p-nitrophenyl esters. Additionally, PsEstA hydrolyzed nitrocefin, cefotaxime, and 7-aminocephalosporanic acid. Interestingly, two forms of immobilized PsEstA (CLEAs-PsEstA and mCLEAs-PsEstA) showed high recycling property and enhanced stability, but hybrid nanoflowers (hNFs) of PsEstA require improvement. This study provides a molecular understanding of substrate specificities, catalytic regulation, and immobilization of PsEstA, which can be efficiently used in biotechnological applications.

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9

Cruz-Izquierdo, Álvaro, Enrique A. Picó, Carmen López, Juan L. Serra, and María J. Llama. "Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica Lipase: An Efficient and Stable Biocatalyst for Biodiesel Synthesis." PLoS ONE 9, no. 12 (2014): e115202. http://dx.doi.org/10.1371/journal.pone.0115202.

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10

Bounegru, Alexandra Virginia, and Constantin Apetrei. "Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review." Nanomaterials 13, no. 4 (2023): 760. http://dx.doi.org/10.3390/nano13040760.

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The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability to catalyze the oxidation of numerous types of phenolic compounds with antioxidant and neurotransmitter roles. This review critically examines the main tyrosinase immobilization techniques for the development of sensitive electrochemical biosensors. Immobilization strategies are mainly classified according to the degree of reversibility/irreversibility of enzyme binding to the support material. Each tyrosinase immobilization method has advantages and limitations, and its selection depends mainly on the type of support electrode, electrode-modifying nanomaterials, cross-linking agent or surfactants used. Tyrosinase immobilization by cross-linking is characterized by very frequent use with outstanding performance of the developed biosensors. Additionally, research in recent years has focused on new immobilization strategies involving cross-linking, such as cross-linked enzyme aggregates (CLEAs) and magnetic cross-linked enzyme aggregates (mCLEAs). Therefore, it can be considered that cross-linking immobilization is the most feasible and economical approach, also providing the possibility of selecting the reagents used and the order of the immobilization steps, which favor the enhancement of biosensor performance characteristics.

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11

Boyle, Elizabeth A., and Tony Anderson. "Validity and Utility of Mclean’s Attitudes to Achievement Questionnaire." Scottish Educational Review 38, no. 1 (2006): 37–53. http://dx.doi.org/10.1163/27730840-03801005.

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McLean (2003a) described a model of motivation for practical use in Scottish schools. An important part of this model concerns attitudes to achievement, defined as sets of beliefs influencing how students go about learning. McLean’s model is similar to Elliot’s achievement goal theory which identifies mastery/performance and approach/avoidance as providing two main distinctions in understanding goals (Elliot, 1999). The aim of the current study was to assess the construct validity and utility of McLean’s attitudes to achievement questionnaire and examine overlaps between attitudes to achievement and Elliot’s achievement goals. 141 Scottish first year undergraduates completed both questionnaires. A four-factor solution did emerge in item level factor analysis, although many items did not load as predicted by McLean. Parallels were found between the approach scales from the two questionnaires but the focus of the avoidance scales was different. Only avoidance scales were linked to academic performance, suggesting that avoidance merits further consideration.

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12

Rana, Nripendra P., Yogesh K. Dwivedi, and Michael D. Williams. "Evaluating the Validity of IS Success Models for the Electronic Government Research." International Journal of Electronic Government Research 9, no. 3 (2013): 1–22. http://dx.doi.org/10.4018/jegr.2013070101.

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The purpose of this paper is to compare and evaluate the validity of Information systems (IS) success models such as DeLone and McLean’s (1992), Seddon’s (1997), and DeLone and McLean’s (2003) IS success models for an e-government application called the Online Public Grievance Redressal System (OPGRS) in context of India. The paper also provides an integrated model of IS success based on the comparison of the three well-known IS success models. All the existing models of IS success are not considered in their original shapes as this research is not using the variables such as use, individual impact, organizational impact from DeLone and McLean (1992) IS success model, use and net benefits from DeLone and McLean (2003) IS success model, and other measures of net benefits of IS use including net benefits to individuals, organizations, and society, and expectations about the net benefits of future IS use from Seddon’s (1997) IS success model. The proposed model contains the additional constructs relevant to e-government research including complexity, trust, and facilitating conditions and found that the model performs quite satisfactorily as far as the success of the OPGRS system is concerned. The paper also discusses its limitations, provides implications for theory and practice, and proposed future lines of research which will allow the researchers, practitioners, and government to leverage the full potential of the OPGRS system to curb and eradicate corruption and to build a transparent and sanitized society.

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13

Walters, Colin. "Jackie McLean "Bird Lives": A Pedagogical Analysis and Application." Jazz Education in Research and Practice 5, no. 1 (2024): 175–84. http://dx.doi.org/10.2979/jazzeducrese.5.1.14.

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Abstract: Jackie McLean composed "Bird Lives" in admiration of the legendary alto saxophonist Charlie Parker. First recorded on McLean's 1990 album, Dynasty, featuring the Jackie McLean Quintet, "Bird Lives" is a classic example of the melodic, harmonic, and rhythmic sounds of the bebop era with a contemporary twist. This article provides practical approaches to learning to performing "Bird Lives" so that musicians and listeners of all ages are able to engage in the communal music making experience of the blues and to embrace the deep oral and aural traditions of the jazz idiom. "Bird Lives" presents learners with opportunities to expand their interpretive and conceptual understanding of bebop articulation and chordal substitutions through their improvisation. Whether performed in a chamber group or large ensemble format, the opportunities to swing, improvise, and create freely are limitless. By studying the innovative stylistic aspects of the bebop era through "Bird Lives" young musicians may begin to develop their own personal sound that reflects the most expressive aspects of the jazz continuum.

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14

Haug, M. "Curious stories surrounding the discovery and development of conventional antithrombotics." Phlebologie 43, no. 02 (2014): 93–98. http://dx.doi.org/10.12687/phleb2179-2-2014.

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ZusammenfassungDie Entdeckung eines therapeutischen Effektes war früher meist ein Zufallsereignis. Vielfach war der Weg bis zur Anwendung lang und steinig und nicht selten von merkwürdigen Geschichten begleitet, so bei den Antithrombotika Heparin, Aspirin und Warfarin.Heparin: Wem gebührt die Anerkennung als Entdecker? Dem Studenten Jay McLean, der neben den gesuchten thromboplastisch wirksamen Cephalin-Komponenten zufällig eine gerinnungshemmende Komponente fand? Oder seinem Chef, der die „zufällige Entdeckung“ McLeans später aufgriff und eben-falls gerinnungshemmende Komponenten isolierte, die sich jedoch chemisch von den McLean´schen unterschieden und zur Ausgangssubstanz des heutigen Heparins wurden?Aspirin: Als schmerz- und fiebersenkendes Mittel längst bekannt, erfuhr es eine zweite Karriere in der kardiovaskulären Medizin. Erst 1967 erkannte man, dass die Plättchenaktivität inhibiert wird. Die zweite Karriere begann mit der Zulassung für TIA und Herzinfarkt anfangs 1980. Bis dato unbekannt war die Geschichte des Dr. Craven, der bereits 1950 in „obskuren“ Zeitschriften seine Beobachtung publizierte, dass alle seine unter Aspirin stehenden Patienten von Herzinfarkt verschont blieben.Warfarin:Um 1920 starben tausende Rinder in Nordamerika an inneren Blutungen. Die Ursache fand man im Verzehr des gelagerten „sweet clover“. Erst nach Jahren hatte man die Erklärung: die Umwandlung des ungiftigen Coumarin im Heu in das gerinnungshemmende Dicoumarol durch Pilzinfektion. Bald folgte die Synthese und damit auch der klinische Einsatz als Antithrombotikum. Viel größeren Erfolg aber hatte die Substanz als Rattengift mit dem Namen Warfarin. Die erneute Anwendung beim Menschen erfolgte erst wieder, nachdem ein Soldat seinen Suizidver-such durch Vitamin K-Gabe überstanden hatte.

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15

Patel, Anand C., Jeffrey D. Morton, Edy Y. Kim, et al. "Genetic segregation of airway disease traits despite redundancy of calcium-activated chloride channel family members." Physiological Genomics 25, no. 3 (2006): 502–13. http://dx.doi.org/10.1152/physiolgenomics.00321.2005.

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Complex airway diseases such as asthma and chronic obstructive pulmonary disease exhibit stereotyped traits (especially airway hyperreactivity and mucous cell metaplasia) that are variably expressed in each patient. Here, we used a mouse model for virus-induced long-term expression of these traits to determine whether individual traits can be genetically segregated and thereby linked to separate determinants. We showed that an F2 intercross population derived from susceptible and nonsusceptible mouse strains can manifest individual phenotypic extremes that exhibit one or the other disease trait. Functional genomic analysis of these extremes further indicated that a member of the calcium-activated chloride channel ( CLCA) gene family designated mClca3 was inducible with mucous cell metaplasia but not airway hyperreactivity. In confirmation of this finding, we found that mClca3 gene transfer to mouse airway epithelium was sufficient to induce mucous cell metaplasia but not airway hyperreactivity. However, newly developed mClca3−/−mice exhibited the same degree of mucous cell metaplasia and airway hyperreactivity as wild-type mice. Bioinformatic analysis of the Clca locus led to the identification of mClca5, and gene transfer indicated that mClca5 also selectively drives mucous cell metaplasia. Thus, in addition to the capacity of CLCA family members to exhibit diverse functional activities, there is also preserved function so that more than one family member mediates mucous cell metaplasia. Nonetheless, Clca expression appears to be a selective determinant of mucous cell metaplasia so that shared homologies between CLCA family members may still represent a useful target for focused therapeutic intervention in hypersecretory airway disease.

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Guly, H. R. "Human biology investigations during the heroic age of Antarctic exploration (1897–1922)." Polar Record 50, no. 2 (2013): 183–91. http://dx.doi.org/10.1017/s0032247412000526.

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ABSTRACTThis article describes the human biological data that was collected from the participants of the Antarctic expeditions of the ‘heroic age’ (1897–1922). Routine body weights, waist circumference, spirometry and grip strength measurements were performed on a number of the expeditions for monitoring health and at least four expeditions measured the haemoglobin level and demonstrated that anaemia did not occur. The only true research was done by Dr A.L. McLean of the Australasian Antarctic Expedition, who measured hair and nail growth and investigated immunity. By modern standards, the data collected is of little value but McLean's work laid the groundwork for modern research into the effects of Antarctic residence on the human organism.

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A.R., Muh Ihsan, and Azwar Azwar. "ANALISIS FAKTOR-FAKTOR YANG MEMENGARUHI KESUKSESAN IMPLEMENTASI SISTEM E-FILING PAJAK." Jurnal BPPK : Badan Pendidikan dan Pelatihan Keuangan 11, no. 2 (2018): 12–34. http://dx.doi.org/10.48108/jurnalbppk.v11i2.348.

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In order to address the task and function in tax policy through the information technology, the Directorate General of Tax, Ministry of Finance of Indonesia, has already developed and launched e-Filing system as tax services in web based. Although it has massively run, there are still problems in its implementation. Hence, we need to evaluate and judge how its implementation based on point of view of its user. The general purpose of this research is to evaluate the success of e-Filing system implementation based on user perception with DeLone & McLean Information System Success Model Approach (1992). By using primary data form questionnaire and secondary data with quantitative approach of Structural Equation Modelling (SEM) based on Partial Least Square (PLS)’s component or variance, this research examine the effect of success indicators measurement on implementation of e-Filing system based on Delone & McLean (1992) model. This model uses six variables which are system quality, information quality, user satisfaction, use, individual impact and organizational impact. This research empirically showed that the e-Filing system currently implementated is not successfully running yet based on all DeLone & McLean’s success measurement criterias. Hence, Directorate General of Tax need to improve, strengthen, and expand the e-Filing system implementation.

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McLean, Stuart, Paul Raine, Geoffrey Pinchbeck, Laura Hunston, YoungAe Kim, and Shotaro Ueno. "The Internal Consistency and Accuracy of Automatically Scored Written Receptive Meaning-Recall Data: A Preliminary Study." Vocabulary Learning and Instruction 10, no. 2 (2021): 64–81. http://dx.doi.org/10.7820/vli.v10.2.mclean.

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Vocableveltest.org is a testing platform on which users can create on- line self-marking meaning-recall (reading or listening) and form-recall (typing) tests that address a number of limitations of the existing vocabulary level tests and vocabulary size tests. A major limitation of many existing vocabulary tests is the written receptive meaning-recognition (multiple-choice or matching) format which is associated with increased error due to guessing and decreased power to measure the type of vocabulary knowledge suitable for reading practice (McLean et al., 2020; Stewart et al., 2021a; Stoeckel et al., 2021), despite being designed for this purpose (Nation, 2012; Schmitt et al., 2020; Webb et al., 2017). Conversely, scoring meaning-recall tests by hand is labour-intensive, and the internal consistency and accuracy of automatically marked data are unknown. Thus, this study investigated the internal consistency and accuracy of automatically marked responses of 98 words from the fifth 100 most frequent words of English. This study tested for knowledge of high-frequency words as a more robust test of the marking system, as these words possess multiple-meaning senses, making their automatic marking problematic. Furthermore, the predicted limited range of learners’ knowledge of these 98 words was expected to result in data of a low internal consistency. However, the automatically marked data had a high internal consistency (Cronbach’s α = 0.868) and was 98% similar to human marked meaning-recall responses.

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Widjaja, Andree E., Jengchung Victor Chen, and Bayarjargal Gonchig. "Investigating Factors Affecting Central Bank Information Systems Success." International Journal of Technology and Human Interaction 14, no. 4 (2018): 43–62. http://dx.doi.org/10.4018/ijthi.2018100103.

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This article investigates the factors affecting the success of central bank information systems (IS) in Mongolia. Drawing from the DeLone and McLean (2003) IS success models and Seddon and Kiew's (1997) path model, the factors that could potentially affect the success of the external and the internal IS of the central bank of Mongolia were empirically investigated. The data analysis was conducted using a quantitative method for the external IS and a mixed method for the internal IS. This article contributes to the academic literature by integrating DeLone and McLean's (2003) IS success model with Seddon and Kiew's (1997) path model and by validating the two aforementioned models within the context of central bank information systems in a developing country. This article has also important practical contributions, particularly for the central bank of Mongolia's authorities. The results, discussions, and implications of this study are elaborated in the paper.

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Gökçe, Gökçen, Volkan Hürmeriç, and Osman Melih Ceylan. "Bir Olgu Nedeniyle Brown-McLean Sendromu." Türk Oftalmoloji Dergisi 43, no. 5 (2013): 365–67. http://dx.doi.org/10.4274/tjo.71602.

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Sorongan, Erick, and Qory Hidayati. "Integration of eucs variables into delone and mclean models for e-government evaluation: Conceptual models." Register: Jurnal Ilmiah Teknologi Sistem Informasi 6, no. 1 (2020): 33. http://dx.doi.org/10.26594/register.v6i1.1608.

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This research was based on the modification of the DeLone and McLean information systems models by adding end-user computing satisfaction variables to determine the success factors for e-government systems. This model was adopted due to the aim of this study to investigate the factors responsible for the successful implementation of e-government by bringing it closer to public value. However, while the DeLone and McLean models focus more on the information system approach, the model proposed was on the premise that system quality (SQ), information quality (IQ), content (CO) and format (FO) are determinants of e-government system user satisfaction. Furthermore, the net benefits through a five-dimensional public value determinants were used to evaluate e-government websites from a community perspective. Responses from 150 communities were analyzed by smart PLS 3.0 using structural equation models to examine the relationship between the constructs of the proposed model. This study contributes to the research gap in adopting DeLone and McLean's model in the e-government due to the limitation in its validation for different contexts. The results support the effect of content variables on user satisfaction and simultaneously prove that it is possible to explain net benefits, with an r-squared value of 69.1%, using the variables in the proposed model. The five dimensions of public value adopted all proved to have a positive influence with a confidence level of 95%. The level of construct significance identified is able to help in the formulation of strategies to improve e-government services.

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Bobadilla Caballero, Manaen Esteban, and Abdiel Osvan Pino. "Malcom McLean." Prisma Tecnológico 10, no. 1 (2019): 48–51. http://dx.doi.org/10.33412/pri.v10.1.2177.

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El hombre que inventó el primer contenedor de la historia enfrentó retos y obstáculos, superando todos y cada uno de ellos con gran tenacidad. Su legado ha sido un ejemplo de lucha, perseverancia, basado en la observación y recolección de datos bajo la perspectiva innovadora, que son elementos que marcan la historia. La trayectoria de Malcom McLean es el retrato de la perseverancia y la voluntad de realizar cambios, partiendo de la brillante e innovadora idea de implementar contenedores, un aspecto que podría verse como simple, pero de gran transcendencia para el transporte logístico. El inventor de la contenedorización no se imaginó cómo su proyecto impulsaría el crecimiento de la logística del transporte y el desarrollo del concepto de estandarización, como también el beneficio de aplicar la economía de escala, de allí que la contenedorización llegaría a convertirse en una de las bases importantes de la globalización al aumentar el traslado de productos de manera segura a un menor costo económico.

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Gibbons, T. B., T. M. Pollock, P. D. Lee, and R. C. Reed. "Malcolm McLean." Materials Science and Technology 25, no. 2 (2009): 127–28. http://dx.doi.org/10.1179/174328409x404860.

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McLean, Will. "McLeans Nuggets." Architectural Design 77, no. 2 (2007): 140–41. http://dx.doi.org/10.1002/ad.438.

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McLean, Will. "McLeans Nuggets." Architectural Design 77, no. 3 (2007): 138–39. http://dx.doi.org/10.1002/ad.474.

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Puspitasari, Tri, Andriani Kusumawati, and Sujarwoto Sujarwoto. "Aplikasi Model DeLone and McLean untuk Mengukur Keberhasilan Sistem Informasi Penelitian dan Pengabdian Masyarakat di Universitas Brawijaya." JURNAL SISTEM INFORMASI BISNIS 10, no. 1 (2020): 94–104. http://dx.doi.org/10.21456/vol10iss1pp94-104.

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Aplication of DeLone and Mclean's Model (2003) renewed is very appropriate measuring the success of information system. This study aims to identify key success factors in the net benefits of information system used for managing research and community services at Brawijaya University. We applied DeLone and McLean model for identifying the key factors of the information system. Data were collected through survey involved 250 University Brawijaya lecturers. The results of the Structural Equation Modeling (SEM) analysis showed the net benefits of the information system were determined by the satisfaction and intention of the lecturers to use the information system, meaning that the more satisfied the lecturers were with the information system and the greater their intention to use the existing information system, the net benefit received would be bigger. Among the three factors that determine the satisfaction and intention of lecturers to use the system, the quality of the system has a greater influence than the quality of information and services.

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., Deny, and Johanes Fernandes Andry. "Pengukuran Keberhasilan E-Learning dengan Mengadopsi Model Delone & Mclean." JURNAL SISTEM INFORMASI BISNIS 8, no. 1 (2018): 68. http://dx.doi.org/10.21456/vol8iss1pp68-75.

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Investment resources, time and cost in the implementation of information systems are not small, making measuring the success of information systems becomes very important to do. Meanwhile, the university as an educational institution today has been rampant to implement e-learning as an information system that provides added value for students. A University in North Jakarta has implemented e-learning integrated with information portal of students and lecturers since 2009. But until now has not been measured the success of the information system. So this research is done with one of the goals to measure the success of e-learning adopted by the University. In addition, this research would like to test the success model of DeLone and McLean's updated information system (2003) in the case of e-learning. The analysis was performed using SEM with the help of Lisrel 8.7 which tested 7 hypotheses. From this research found that DeLone and McLean model can not be used to explain the cause of success of e-learning system that its use is required, but need further research to get better level of generalization.

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Rahimi, Amir Reza, and Zahra Mosali. "The post-pandemic intentions of EFL lecturers towards online language teaching." World Journal on Educational Technology: Current Issues 16, no. 2 (2024): 85–102. http://dx.doi.org/10.18844/wjet.v16i2.9001.

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After the widespread transition to Emergency Remote Language Teaching , there is a need to investigate teachers' continued intention toward online language teaching post-pandemic. Thus, DeLone and McLean's model of information systems success was used to investigate university lecturers' attitudes toward OLT during the post-pandemic period. The maximum variation sampling was conducted on fourteen university lecturers from various cities in Iran. A three-phase interview protocol was developed and applied to the participants. The data was analyzed with content analysis. The result of the deductive content analysis showed that OLT helped university instructors overcome technophobia and become experts in it. However, OLT had some health hazards for lecturers and led students to passive learning. Furthermore, the lack of technical, financial, and professional support from the administrators affected OLT service quality. Moreover, participants would have blended and flipped language teaching during the post-pandemic, based on learners' levels and psychological factors. Among many implications, the study suggests more teacher training workshops for university lecturers to overcome technophobia. Keywords: DeLone and McLean information systems success model; EFL teachers; Online Language Teaching; Post-pandemic.

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Yeping, Hu. "George Francis McLean." Dialogue and Universalism 17, no. 7 (2007): 61–73. http://dx.doi.org/10.5840/du2007177/817.

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Sawers, Ronald, and Bernard Gilligan. "John Angus McLean." Medical Journal of Australia 153, no. 9 (1990): 559. http://dx.doi.org/10.5694/j.1326-5377.1990.tb126201.x.

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Repp, John. "Mingus and McLean." Iowa Review 29, no. 3 (1999): 139–40. http://dx.doi.org/10.17077/0021-065x.5213.

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McLean, Alan A. "McLean??s Comment." Journal of Occupational and Environmental Medicine 27, no. 11 (1985): 792. http://dx.doi.org/10.1097/00043764-198511000-00008.

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McLean, M. "Robert Donald McLean." BMJ 347, sep09 18 (2013): f5181. http://dx.doi.org/10.1136/bmj.f5181.

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Bayliss, C. "Malcolm Downie McLean." BMJ 325, no. 7372 (2002): 1118a—1118. http://dx.doi.org/10.1136/bmj.325.7372.1118/a.

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Purdie, G. "Iain Cran McLean." BMJ 339, sep11 1 (2009): b3740. http://dx.doi.org/10.1136/bmj.b3740.

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Lindenfeld, Peter. "William L. McLean." Physics Today 45, no. 3 (1992): 82. http://dx.doi.org/10.1063/1.2809594.

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Jacobs, Jennifer, and Sara Schamerloh. "BROWN-MCLEAN SYNDROME." Optometry and Vision Science 79, Supplement (2002): 79. http://dx.doi.org/10.1097/00006324-200212001-00148.

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Kam, K. W., V. Jhanji, and A. L. Young. "Brown-McLean syndrome." Case Reports 2013, oct09 1 (2013): bcr2013201280. http://dx.doi.org/10.1136/bcr-2013-201280.

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Fau, C., S. Nabzo, A. Hodali, and T. Hodali. "Tonómetro de McLean." Archivos de la Sociedad Española de Oftalmología 95, no. 2 (2020): e9-e12. http://dx.doi.org/10.1016/j.oftal.2019.07.001.

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Vogel, Mark S., Tamara Petrosyan, Brent T. Chin, Aleksandra Wienecka, Lesanh Trinh, and Tracy Goldstein. "Brown-McLean syndrome." Optometry - Journal of the American Optometric Association 82, no. 8 (2011): 485–88. http://dx.doi.org/10.1016/j.optm.2010.10.015.

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Lim, Jennifer I. "Brown-McLean Syndrome." Archives of Ophthalmology 109, no. 1 (1991): 22. http://dx.doi.org/10.1001/archopht.1991.01080010024016.

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Trie Sartika Pratiwi and Azrai’e Kamaludin Rosni. "IMPLEMENTASI KNOWLEDGE MANAGEMENT : MENGUJI PENGARUH FAKTOR-FAKTOR TERHADAP KINERJA KARYAWAN." Equivalent : Journal Of Economic, Accounting and Management 1, no. 1 (2023): 09–21. http://dx.doi.org/10.61994/equivalent.v1i1.24.

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In information systems research DeLone and McLean (1992, 2003) is a model that can be used as a reference tool in measuring the success of information systems. This model is considered to represent each dimension measurement of the success of information systems applications. This study refers to and modifies the model of success DeLone and McLean (2003) to measure the success of the application of KMS through employee performance variables. In this study researchers included several factors that are taken from the model of DeLone and McLean (2003), models of Davis (1989) as well as models of Igbaria et al., (1997). Abstrak Dalam penelitian sistem informasi DeLone dan McLean (1992, 2003) merupakan model yang dapat dijadikan sebagai alat acuan dalam mengukur keberhasilan sistem informasi. Model ini dianggap mewakili setiap dimensi pengukuran keberhasilan aplikasi sistem informasi. Penelitian ini mengacu dan memodifikasi model kesuksesan DeLone dan McLean (2003) untuk mengukur keberhasilan penerapan KMS melalui variabel kinerja karyawan. Dalam penelitian ini peneliti memasukkan beberapa faktor yang diambil dari model DeLone dan McLean (2003), model Davis (1989) serta model Igbaria et al., (1997)

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Setyaningtyas, Adella Ayu, Muhammad Labib Jundillah, and Vina Zahrotun Kamila. "Penggunaan Metode Delone dan Mclean Untuk Menganalisis Kesuksesan Mulawarman Online Learning System (MOLS)." Adopsi Teknologi dan Sistem Informasi (ATASI) 2, no. 2 (2023): 110–18. http://dx.doi.org/10.30872/atasi.v2i2.1057.

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In 2019, the Mulawarman Online Learning System (MOLS) was created. All Mulawarman University students can use MOLS for support their study. The purpose of this reasearch is to determine the level of success in using the Mulawarman Online Learning System (MOLS) using the Delone and Mclean method. In this study using the Delone and Mclean method. Delone and Mclean are methods for measuring the success rate of users of an information system. Delone and Mclean have 6 (six) measurement variables including system quality, information User Satisfaction Delone and Mclean E-Learning quality, user satisfaction, use, service quality and net benefit. This research is quantitative data with a sample of 100 Mulawarman University student respondents. The results of this study indicate that the independent variables, namely system quality, information quality and service quality can affect the dependent variable, namely user satisfaction, use and net benefits.

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Dhian Nur Rahayu and Lila Setiyani. "Systematic Literature Review: Delone and Mclean Model using VOSViewer on Google Scholar Database Case Year 2010-2020." International Journal of Science, Technology & Management 3, no. 1 (2022): 22–30. http://dx.doi.org/10.46729/ijstm.v3i1.402.

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This study aims to examine the development of the Delone and Mclean information system success model. This research was conducted to determine: (1) a map of the development of the number of publications on the Delone and Mclean model; (2) find out which scientific articles have the highest number of citations; (3) knowing the development map of scientific publications based on keywords. The data sources used in this study are scientific articles in the Google Scholar database with the keywords Delone and Mclean, with the categories article title, abstract, keywords in the 2016-2020 range. The search results show that there are 270 scientific articles that match the keywords based on the Harzing's Publish or Perish application. The results showed that the development of the Delone and Mclean model in 2010 - 2020 which was the highest in the Google Scholar database occurred in 2020 which reached 68 publications or 25%. Meanwhile, the publications that have the highest number of citations are Nils Urbach and Benjamin Müller (2016) which were quoted 379 times. The development map of scientific publications based on the keywords delone and mclean analyzed using the VOSViewer application shows that the development map of the delone and mclean model is divided into 4 clusters. The red cluster 1 consists of 14 items, the green cluster 2 consists of 11 items, the blue cluster 3 consists of 10 items, and the yellow cluster 4 consists of 6 items.

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Chatterjee, Samrat, SwapnilM Parchand, Debashish Dash, and Deepshikha Agrawal. "Brown-McLean syndrome revisited." Indian Journal of Ophthalmology 68, no. 1 (2020): 183. http://dx.doi.org/10.4103/ijo.ijo_1124_19.

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Culliton, B. "Frazier reinstated at McLean." Science 243, no. 4893 (1989): 889. http://dx.doi.org/10.1126/science.2919282.

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Amery, Julian. "Billy McLean: 1918–1986∗." Central Asian Survey 6, no. 2 (1987): 3–6. http://dx.doi.org/10.1080/02634938708400581.

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NHFW and HWP. "John Walford McLean OBE." British Dental Journal 207, no. 4 (2009): 187. http://dx.doi.org/10.1038/sj.bdj.2009.729.

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Oram, E. G. "Alexander Dalrymple McLean Douglas." Psychiatric Bulletin 17, no. 4 (1993): 252. http://dx.doi.org/10.1192/pb.17.4.252.

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Chan, Kai Chiu. "Response to Dr, McLean." Journal of Esthetic and Restorative Dentistry 4, no. 6 (1992): 222. http://dx.doi.org/10.1111/j.1708-8240.1992.tb00705.x.

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