Academic literature on the topic 'Mean fluorescence intensity'

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Journal articles on the topic "Mean fluorescence intensity"

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Loupy, Alexandre, and Christophe Legendre. "From Mean Fluorescence Intensity to C1q-Binding." Transplantation 99, no. 6 (2015): 1107–8. http://dx.doi.org/10.1097/tp.0000000000000700.

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Jansen, Sanne, Daniel De Bruin, Simon Strackee, et al. "PS01.186: QUANTITATIVE PERFUSION EVALUATION AFTER GASTRIC TUBE RECONSTRUCTION USING FLUORESCENCE IMAGING." Diseases of the Esophagus 31, Supplement_1 (2018): 102–3. http://dx.doi.org/10.1093/dote/doy089.ps01.186.

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Abstract Background Poor fundus perfusion is seen as the major factor for the development of anastomotic necrosis, leakage and strictures. Quantitative imaging of tissue perfusion during reconstructive surgery, therefore, may reduce the incidence of complications. Imaging the fluorescense of intravenously administered fluorophores is an optical, non-contact method to image blood flow in real-time. However, quantitative parameters for perfusion evaluation are stil lacking. The objective of this study is to test fluorescence imaging derived quantitative parameters for perfusion evaluation of the
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A. Wells, Denise, and Michael R. Loken. "Flow cytometric mean fluorescence intensity: The biophysics behind the number." Leukemia Research 32, no. 6 (2008): 845–46. http://dx.doi.org/10.1016/j.leukres.2007.10.002.

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Maryamchik, E., X. Zheng, X. Zhang, et al. "Low CD45 mean fluorescence intensity predicts poor Post-Thaw CD34 cell viability." Cytotherapy 22, no. 5 (2020): S63. http://dx.doi.org/10.1016/j.jcyt.2020.03.093.

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Zhao, J., Y. X. Fu, T. Yang, Z. Y. Shen, and C. L. Wu. "Prediction of Complement-Binding Capacity of HLA Antibodies Based on Mean Fluorescence Intensity." Transplantation Proceedings 48, no. 6 (2016): 2235–40. http://dx.doi.org/10.1016/j.transproceed.2016.04.018.

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Wu, Chang Ni, and Yang Chen. "Lanthanide Complex-Polyethylenimine Conjugate: A Highly Luminescent Probe for Time-Resolved Fluorescence Analysis." Applied Mechanics and Materials 108 (October 2011): 212–16. http://dx.doi.org/10.4028/www.scientific.net/amm.108.212.

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Macromolecular polymer polyethylenimine (PEI) modified by many luminescent lanthanide complexes was prepared and purified. The spectral properties and lifetime of this fluorescence probe were measured. The mean number of lanthanide complexes per PEI molecule was measured and calculated. The fluorescent intensity of a macromolecule modified with lanthanide complexes is 6-fold as high as that of a lanthanide complex molecule.
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Saipetch, K., and C. Yoshimura. "Importance of correcting for fluorescence quenching in fluorescence-based prediction of trihalomethane formation potential." Water Supply 19, no. 6 (2019): 1677–85. http://dx.doi.org/10.2166/ws.2019.039.

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Abstract Fluorescence excitation–emission matrix (EEM) spectroscopy is often used to determine the levels of trihalomethane (THM) precursors in natural organic matter. However, humic substances are known to quench the fluorescence of amino acids and proteins. To date, none of the EEM-based models for predicting THM formation potential (THMFP) have explicitly accounted for these quenching effects. Thus, we investigated the importance of correcting for fluorescence quenching during THMFP prediction. Fluorescence titration experiments revealed that the correction improved the accuracy of THM pred
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Wang, Jun Sheng, You Nan Song, Jin Yang Sun та ін. "A Microfluidic Cytometer for Quantitative Evaluation of Radiation Dose by γ-H2AX". Applied Mechanics and Materials 522-524 (лютий 2014): 1119–22. http://dx.doi.org/10.4028/www.scientific.net/amm.522-524.1119.

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Evaluation of radiation dose is very important for the detection of radiation damage. γ-H2AX is a popular biological dosimeter to evaluate the radiation effect. Typically, bulky and expensive commercial flow cytometers are used to detect γ-H2AX. This paper presents a miniaturized and highly sensitive cytometer using a microfluidic chip for evaluating the radiation dose by detecting the mean immunofluorescence intensity of γ-H2AX. A compact optical focusing system and a shift-phase differential amplifier are designed to improve the detection sensitivity. Sample lymphocyte cells are stained by F
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Frénoy, J. P., F. Emmanuel, and J. M. Salmon. "Use of quantitative image microfluorometry to follow fluorescent ricin internalization in single living cells." Journal of Histochemistry & Cytochemistry 42, no. 5 (1994): 627–33. http://dx.doi.org/10.1177/42.5.8157934.

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We used microspectrofluorometry and videomicrofluorometry to follow the binding and internalization of fluorescein-labeled toxic lectin ricin in living Zajdela hepatoma cells. Microspectrofluorometry showed that when ricin was specifically labeled on its B-chain with one molecule of fluorescein (ABF), its fluorescence spectrum did not alter during its binding to the cell surface and subsequent internalization. This enabled us to use image analysis to follow cell internalization of labeled ricin. Accordingly, we measured the appropriate fluorescent cell parameters, comprising total fluorescence
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Baker, A., R. Inverarity, and D. Ward. "Catchment-scale fluorescence water quality determination." Water Science and Technology 52, no. 9 (2005): 199–207. http://dx.doi.org/10.2166/wst.2005.0319.

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Chemical water quality determinants and river water fluorescence were determined on the River Tyne, northeast England. Statistically significant relationships between nitrate (r=0.87), phosphate (r=0.80), ammonia (r=0.70), biochemical oxygen demand (BOD) (r=0.85) and dissolved oxygen (r=−0.65) and tryptophan-like fluorescence intensity were observed. The strongest correlations are between tryptophan-like intensity and nitrate and phosphate, which in the Tyne catchment derive predominantly from point and diffuse source sewage inputs. The correlation between BOD and the tryptophan-like fluoresce
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Dissertations / Theses on the topic "Mean fluorescence intensity"

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Janíček, Tomáš. "Vliv hybridizačních směsí na intenzitu fluorescence při in situ hybridizaci." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2019. http://www.nusl.cz/ntk/nusl-401944.

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Fluorescenční in situ hybridizace (FISH) je široce používaná metoda pro detekci určité sekvence DNA na chromozomech. Cílem práce je porovnání tří různých chemických sloučenin (formamidu, ethylenkarbonátu a sodných kationtů) používaných ve směsích pro in situ hybridizaci. Složení těchto směsí ovlivňuje renaturaci DNA a je důležité porovnat jejich fyzikální vlastnosti. Práce je rozdělena do dvou hlavních částí. První část se zabývá otázkou termodynamických parametrů používaných pro experimenty FISH, jako je teplota tání, entalpie přechodu DNA ze dvoušroubovice na vlákno nám dává přehled o energi
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Book chapters on the topic "Mean fluorescence intensity"

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Eftink, Maurice R., and Glen D. Ramsay. "Temperature- and Pressure-Induced Unfolding of a Mutant of Staphylococcal Nuclease A." In High Pressure Effects in Molecular Biophysics and Enzymology. Oxford University Press, 1996. http://dx.doi.org/10.1093/oso/9780195097221.003.0008.

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Nuclease conA is a hybrid version of Staphylococcal nuclease that contains a six amino acid (β-turn substitute from concanavalin A. This hybrid protein has a much lower thermodynamic stability than does the wild-type protein. This enables the unfolding of the protein to be achieved easily by several types of perturbations. From temperature-, pressure-, and denaturant-induced unfolding studies, we have found the free energy change for unfolding, ΔG°un, to be approximately 1.4 kcal mo−1 at pH 7, 0.1 M NaCI, and 20 °C, as compared to a thermodynamic stability of approximately 5.5–6 kcal mo−1 for wild-type nuclease A. Due to its reduced thermodynamic stability, nuclease conA also shows evidence of unfolding at low-temperature (cold denaturation), with a temperature of maximum stability of 13–15 °C. The thermal unfolding of nuclease conA is shown to be two-state by simultaneous measurement of fluorescence and CD changes as a function of temperature, using a modified AVIV CD instrument. Increased hydrostatic pressure unfolds nuclease conA in what appears to be a two-state manner, with an apparent of ΔV°un approximately —100 ml mol−1. From studies of the pressure (p)-induced unfolding of this hybrid protein as a function of temperature (T), we can define the complete p-T free energy surface for the unfolding transition. In auxiliary studies, we have characterized the fluorescence intensity decay and anisotropy decay of the single tryptophan residue (Trp-140) of nuclease conA in the native state and in the unfolded state induced by temperature, pressure, and denaturant. For each type of perturbation, there is a red shift in fluorescence, a lowering of the mean fluorescence lifetime, and a lowering of the rotational correlation time of the tryptophan residue to a value of ~1 ns (compared to 10–15 ns for the native state). The thermodynamics of the unfolding of proteins has received renewed interest in recent years, owing to the availability of a rich variety of mutant proteins and to advances in our understanding of their structural features. Among the questions being asked are, What are the relative energetic contributions of the hydrophobic effect and other interaction forces?
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Conference papers on the topic "Mean fluorescence intensity"

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Startceva, Sofia, Jerome G. Chandraseelan, Ari Visa, and Andre S. Ribeiro. "Quantitative Estimation of Long-living Fluorescent Molecules from Temporal Fluorescence Intensity Data Corrupted by Nonzero-mean Noise." In 9th International Conference on Bio-inspired Systems and Signal Processing. SCITEPRESS - Science and and Technology Publications, 2016. http://dx.doi.org/10.5220/0005605900170024.

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Suzuki, Hiroki, Kouji Nagata, Yasuhiko Sakai, and Ryota Ukai. "An Experimental Study on Turbulent Mixing of High-Schmidt-Number Scalar in Grid Turbulence by Means of PIV and PLIF." In ASME-JSME-KSME 2011 Joint Fluids Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajk2011-21013.

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Turbulent mixing of high-Schmidt-number passive scalar in shear-free grid turbulence is experimentally investigated using a water channel. The Reynolds number based on the mesh size of the grid and cross-sectionally averaged mean velocity is 2,500. Rhodamine B (fluorescent dye) was used as a high-Schmidt-number passive scalar. The Schmidt number is about 2,100. The time-resolved particle image velocimetry (PIV) and the planar laser induced fluorescence (PLIF) technique were used to measure instantaneous two-component velocities and nondimensional concentration. Our PLIF algorithm corrects the
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Doublet, Pierre, Christine Lempereur, Virginel Bodoc, Mikael Orain, and Pierre Gajan. "Planar droplet sizing: Application to a spray of Jet A1 kerosene." In ILASS2017 - 28th European Conference on Liquid Atomization and Spray Systems. Universitat Politècnica València, 2017. http://dx.doi.org/10.4995/ilass2017.2017.4698.

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Optical techniques are widely employed for their non-intrusive behavior and are applied to two-phase flowinvestigations. Until now, the most commonly used technique to determine the droplet size is the Phase Doppler Anemogranulometry, although it is time consuming for an overall injector characterization. An imaging technique called Planar Droplet Sizing has been used to offer an alternative and provide a spatially-resolved 2D map of the Sauter Mean Diameter (SMD). The measurement is based on the ratio between laser-induced fluorescence and scattered light intensities which are assumed to be p
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Griebel, P., R. Scha¨ren, P. Siewert, R. Bombach, A. Inauen, and W. Kreutner. "Flow Field and Structure of Turbulent High-Pressure Premixed Methane/Air Flames." In ASME Turbo Expo 2003, collocated with the 2003 International Joint Power Generation Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/gt2003-38398.

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The present study focuses on the flow field characterization of highly turbulent premixed flames, typical for stationary gas turbines. Mean flame front position and flame front structure at high inlet temperatures, lean mixtures, and high pressures are studied, too. Turbulence intensities and integral length scales have been measured in an isothermal flow field with the help of Particle Image Velocimetry (PIV). Mean flame front position and flame structure have been studied using Planar Laser-Induced Fluorescence (PLIF) of the OH radical. Turbulence intensities and integral length scales have
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Kitagawa, Atsuhide, Kazuyasu Sugiyama, Masa-aki Ashihara, Koichi Hishida, and Yoshiaki Kodama. "Measurement of Turbulence Modification by Microbubbles Causing Frictional Drag Reduction." In ASME/JSME 2003 4th Joint Fluids Summer Engineering Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/fedsm2003-45648.

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In this research, in order to clarify the mechanism for drag reduction caused by microbubbles, the turbulence structure of flow field including microbubbles in a horizontal channel is experimentally investigated using particle tracking velocimetry and laser induced fluorescence (PTV/LIF) technique. First, we discuss the particle image velocimetry (PIV) and PTV results for the liquid phase velocity detection. Second, using instantaneous measurement data, we obtain the profiles of the mean velocity, turbulence intensity and Reynolds stress of the liquid phase. In order to obtain the information
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Upadhyay, Jagannath, Daniel S. Park, Karsten E. Thompson, and Dimitris E. Nikitopoulos. "3D Measurements of Nano-Particle Transport in Complex 2.5D Micro-Models." In ASME 2015 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/imece2015-50635.

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A confocal Micro-Particle Image Velocimetry (C-μPIV) technique along with associated post image processing algorithms is established to quantify three dimensional distributions of nano-particle velocity and concentration at the micro-scale (pore-scale) in 2.5D porous media designed from a Boise rock sample. In addition, an in-situ, non-destructive method for measuring the geometry of the micro-model, including its depth, is described and demonstrated. The particle experiments use 900 nm fluorescence labeled polystyrene particles at a flow rate of 10 nLmin−1 and confocal laser scanning microsco
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Timite, Brahim, Cathy Castelain, and Hassan Peerhossaini. "Pulsating Flow for Mixing Intensification in a Twisted Curved Pipe." In ASME/JSME 2007 5th Joint Fluids Engineering Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/fedsm2007-37065.

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This work concerns the manipulation of a twisted curved pipe flow for mixing enhancement. Previous work [1,2,3] has shown that geometrical perturbations to a curved pipe flow can increase mixing and heat transfer by chaotic advection. In this work the flow entering the twisted pipe undergoes a pulsatile motion. The flow was studied experimentally and numerically. The numerical study is carried out by CFD code (Fluent 6) in which a pulsated velocity field is imposed as an inlet condition. The experimental setup involves principally a “Scotch-yoke” pulsatile generator and a twisted curved pipe.
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Hedman, Paul O., Thomas H. Fletcher, Stewart G. Graham, G. Wayne Timothy, Daniel V. Flores, and Jason K. Haslam. "Observations of Flame Behavior in a Laboratory-Scale Pre-Mixed Natural Gas/Air Gas Turbine Combustor From PLIF Measurements of OH." In ASME Turbo Expo 2002: Power for Land, Sea, and Air. ASMEDC, 2002. http://dx.doi.org/10.1115/gt2002-30052.

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The objective of this study was to obtain instantaneous planar laser induced fluorescence (PLIF) images of OH in a laboratory-scale, gas-turbine combustor (LSGTC) with a pre-mixed, swirl-stabilized, natural gas flame. Instantaneous PLIF images of OH were obtained at each of four operating conditions (high swirl and medium swirl at fuel equivalence ratios of 0.80 and 0.65). Comparison of the instantaneous images illustrates the stochastic nature of the flame structure. Pixel by pixel statistical analysis of each collection of images allowed both mean and standard deviation images to be generate
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Ball, J., M. Greaves, C. Jackson, J. Peel, and F. E. Preston. "DN-9693: A PHOSPHODIESTERASE INHIBITOR WITH A PLATELET MEMBRANE EFFECT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643583.

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We have examined the effect of DN-9693 (piperidinyl - imidazo - quinazoline: Daiichi Seiyaku, Japan) a water soluble phosphodiesterase inhibitor on platelet aggregation, secretion and thromboxane B2 (TXB2) production. In platelet rich plasma and at concentrations of 2uM and 5uM the drug significantly inhibited aggregation induced by adenosine diphosphate, collagen and sodium arachidonate. TXB2 production and release of adenosine tri-phosphate and 14C 5-hydroxytryptamine were also significantly inhibited by the drug. Cyclic adenosine mono-phosphate accumulation was enhanced. Inhibition of risto
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Niibori, Yuichi, Masayuki Narita, Akira Kirishima, Taiji Chida, and Hitoshi Mimura. "Fluorescence Emission Behavior of Eu(III) Sorbed on Calcium Silicate Hydrates Formed With No Dried Process." In 2013 21st International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/icone21-16524.

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Calcium silicate hydrate (CSH) is a main component of cement-based material required for constructing the geological repository. As in many countries, since the repository in Japan is constructed below water table, we must consider the interaction of radionuclide with cement materials altered around the repository after the backfill. Using fluorescence emission spectra, so far, the authors have investigated the interaction of Eu(III) (as a chemical analog of Am(III)) with CSH gels formed with no dried process, considering a condition saturated with groundwater. However, in such fluorescence em
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