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Journal articles on the topic 'Mean fluorescence intensity'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Loupy, Alexandre, and Christophe Legendre. "From Mean Fluorescence Intensity to C1q-Binding." Transplantation 99, no. 6 (2015): 1107–8. http://dx.doi.org/10.1097/tp.0000000000000700.

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2

Jansen, Sanne, Daniel De Bruin, Simon Strackee, et al. "PS01.186: QUANTITATIVE PERFUSION EVALUATION AFTER GASTRIC TUBE RECONSTRUCTION USING FLUORESCENCE IMAGING." Diseases of the Esophagus 31, Supplement_1 (2018): 102–3. http://dx.doi.org/10.1093/dote/doy089.ps01.186.

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Abstract Background Poor fundus perfusion is seen as the major factor for the development of anastomotic necrosis, leakage and strictures. Quantitative imaging of tissue perfusion during reconstructive surgery, therefore, may reduce the incidence of complications. Imaging the fluorescense of intravenously administered fluorophores is an optical, non-contact method to image blood flow in real-time. However, quantitative parameters for perfusion evaluation are stil lacking. The objective of this study is to test fluorescence imaging derived quantitative parameters for perfusion evaluation of the

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3

A. Wells, Denise, and Michael R. Loken. "Flow cytometric mean fluorescence intensity: The biophysics behind the number." Leukemia Research 32, no. 6 (2008): 845–46. http://dx.doi.org/10.1016/j.leukres.2007.10.002.

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4

Maryamchik, E., X. Zheng, X. Zhang, et al. "Low CD45 mean fluorescence intensity predicts poor Post-Thaw CD34 cell viability." Cytotherapy 22, no. 5 (2020): S63. http://dx.doi.org/10.1016/j.jcyt.2020.03.093.

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5

Zhao, J., Y. X. Fu, T. Yang, Z. Y. Shen, and C. L. Wu. "Prediction of Complement-Binding Capacity of HLA Antibodies Based on Mean Fluorescence Intensity." Transplantation Proceedings 48, no. 6 (2016): 2235–40. http://dx.doi.org/10.1016/j.transproceed.2016.04.018.

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6

Wu, Chang Ni, and Yang Chen. "Lanthanide Complex-Polyethylenimine Conjugate: A Highly Luminescent Probe for Time-Resolved Fluorescence Analysis." Applied Mechanics and Materials 108 (October 2011): 212–16. http://dx.doi.org/10.4028/www.scientific.net/amm.108.212.

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Macromolecular polymer polyethylenimine (PEI) modified by many luminescent lanthanide complexes was prepared and purified. The spectral properties and lifetime of this fluorescence probe were measured. The mean number of lanthanide complexes per PEI molecule was measured and calculated. The fluorescent intensity of a macromolecule modified with lanthanide complexes is 6-fold as high as that of a lanthanide complex molecule.

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7

Saipetch, K., and C. Yoshimura. "Importance of correcting for fluorescence quenching in fluorescence-based prediction of trihalomethane formation potential." Water Supply 19, no. 6 (2019): 1677–85. http://dx.doi.org/10.2166/ws.2019.039.

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Abstract Fluorescence excitation–emission matrix (EEM) spectroscopy is often used to determine the levels of trihalomethane (THM) precursors in natural organic matter. However, humic substances are known to quench the fluorescence of amino acids and proteins. To date, none of the EEM-based models for predicting THM formation potential (THMFP) have explicitly accounted for these quenching effects. Thus, we investigated the importance of correcting for fluorescence quenching during THMFP prediction. Fluorescence titration experiments revealed that the correction improved the accuracy of THM pred

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Wang, Jun Sheng, You Nan Song, Jin Yang Sun та ін. "A Microfluidic Cytometer for Quantitative Evaluation of Radiation Dose by γ-H2AX". Applied Mechanics and Materials 522-524 (лютий 2014): 1119–22. http://dx.doi.org/10.4028/www.scientific.net/amm.522-524.1119.

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Evaluation of radiation dose is very important for the detection of radiation damage. γ-H2AX is a popular biological dosimeter to evaluate the radiation effect. Typically, bulky and expensive commercial flow cytometers are used to detect γ-H2AX. This paper presents a miniaturized and highly sensitive cytometer using a microfluidic chip for evaluating the radiation dose by detecting the mean immunofluorescence intensity of γ-H2AX. A compact optical focusing system and a shift-phase differential amplifier are designed to improve the detection sensitivity. Sample lymphocyte cells are stained by F

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9

Frénoy, J. P., F. Emmanuel, and J. M. Salmon. "Use of quantitative image microfluorometry to follow fluorescent ricin internalization in single living cells." Journal of Histochemistry & Cytochemistry 42, no. 5 (1994): 627–33. http://dx.doi.org/10.1177/42.5.8157934.

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We used microspectrofluorometry and videomicrofluorometry to follow the binding and internalization of fluorescein-labeled toxic lectin ricin in living Zajdela hepatoma cells. Microspectrofluorometry showed that when ricin was specifically labeled on its B-chain with one molecule of fluorescein (ABF), its fluorescence spectrum did not alter during its binding to the cell surface and subsequent internalization. This enabled us to use image analysis to follow cell internalization of labeled ricin. Accordingly, we measured the appropriate fluorescent cell parameters, comprising total fluorescence

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10

Baker, A., R. Inverarity, and D. Ward. "Catchment-scale fluorescence water quality determination." Water Science and Technology 52, no. 9 (2005): 199–207. http://dx.doi.org/10.2166/wst.2005.0319.

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Chemical water quality determinants and river water fluorescence were determined on the River Tyne, northeast England. Statistically significant relationships between nitrate (r=0.87), phosphate (r=0.80), ammonia (r=0.70), biochemical oxygen demand (BOD) (r=0.85) and dissolved oxygen (r=−0.65) and tryptophan-like fluorescence intensity were observed. The strongest correlations are between tryptophan-like intensity and nitrate and phosphate, which in the Tyne catchment derive predominantly from point and diffuse source sewage inputs. The correlation between BOD and the tryptophan-like fluoresce

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11

Zimnyakov, Dmitry, Elena Isaeva, Anna Isaeva, and Sergey Volchkov. "Band-Limited Reference-Free Speckle Spectroscopy: Probing the Fluorescent Media in the Vicinity of the Noise-Defined Threshold." Applied Sciences 10, no. 5 (2020): 1629. http://dx.doi.org/10.3390/app10051629.

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A method of reference-free speckle spectroscopy based on the statistical analysis of intensity spatial fluctuations of the spectrally-selected multiple-scattered fluorescence radiation is examined in the case of the finite-band spectral selection of fluorescence light emitted by the laser-pumped random medium, and detection conditions far from the ideal case. Intensity fluctuations are recorded during point-to-point scanning of the surface of a random multiple-scattering medium, which is characterized by the dependences of the second- and third-order statistical moments of intensity on the wav

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12

Claisse, Guillaume, Lena Absi, Eric Alamartine, Nicolas Maillard, and Christophe Mariat. "SO003RELATIONSHIP BETWEEN MEAN FLUORESCENCE INTENSITY AND C1Q/C3D-FIXING CAPACITIES OF ANTI-HLA ANTIBODIES." Nephrology Dialysis Transplantation 31, suppl_1 (2016): i2. http://dx.doi.org/10.1093/ndt/gfw117.03.

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13

Claisse, Guillaume, Lena Absi, Fabrice Cognasse, Eric Alamartine, Christophe Mariat, and Nicolas Maillard. "Relationship between Mean Fluorescence Intensity and C1q/C3d-fixing capacities of anti-HLA antibodies." Human Immunology 78, no. 4 (2017): 336–41. http://dx.doi.org/10.1016/j.humimm.2017.02.003.

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14

Shooshtari, Parisa, Edgardo S. Fortuno, Darren Blimkie, et al. "Correlation analysis of intracellular and secreted cytokines via the generalized integrated mean fluorescence intensity." Cytometry Part A 77A, no. 9 (2010): 873–80. http://dx.doi.org/10.1002/cyto.a.20943.

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15

Lau, Darryl, Shawn L. Hervey-Jumper, Susan Chang, et al. "A prospective Phase II clinical trial of 5-aminolevulinic acid to assess the correlation of intraoperative fluorescence intensity and degree of histologic cellularity during resection of high-grade gliomas." Journal of Neurosurgery 124, no. 5 (2016): 1300–1309. http://dx.doi.org/10.3171/2015.5.jns1577.

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OBJECT There is evidence that 5-aminolevulinic acid (ALA) facilitates greater extent of resection and improves 6-month progression-free survival in patients with high-grade gliomas. But there remains a paucity of studies that have examined whether the intensity of ALA fluorescence correlates with tumor cellularity. Therefore, a Phase II clinical trial was undertaken to examine the correlation of intensity of ALA fluorescence with the degree of tumor cellularity. METHODS A single-center, prospective, single-arm, open-label Phase II clinical trial of ALA fluorescence-guided resection of high-gra

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16

Yin, Wei, Chun Wang, Kuohai Fan, Na Sun, Yaogui Sun, and Hongquan Li. "In vitro observation: the GFP-E. coli adhering to porcine erythrocytes can be removed by porcine alveolar macrophages." PeerJ 7 (March 8, 2019): e6439. http://dx.doi.org/10.7717/peerj.6439.

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Although the activation of pathogen phagocytosis via complement system has been studied, erythrocyte-phagocyte interactions in pigs are not clearly understood. Therefore, we sought to investigate the ability of porcine erythrocytes to clear immune complexes (ICs) by using laser confocal microscopy and flow cytometry to observe the immune adhesion of porcine erythrocytes to fluorescent bacilli and the immune presentation process of transferring fluorescent bacilli to macrophages. Isolated porcine alveolar macrophages (PAMs) had uniform morphology and size, and a survival rate of 97.2%. The phag

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17

Kamp, Marcel A., Philipp Slotty, Bernd Turowski, et al. "Microscope-Integrated Quantitative Analysis of Intraoperative Indocyanine Green Fluorescence Angiography for Blood Flow Assessment: First Experience in 30 Patients." Operative Neurosurgery 70, suppl_1 (2011): ons65—ons74. http://dx.doi.org/10.1227/neu.0b013e31822f7d7c.

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Abstract BACKGROUND: Intraoperative measurements of cerebral blood flow are of interest during vascular neurosurgery. Near-infrared indocyanine green (ICG) fluorescence angiography was introduced for visualizing vessel patency intraoperatively. However, quantitative information has not been available. OBJECTIVE: To report our experience with a microscope with an integrated dynamic ICG fluorescence analysis system supplying semiquantitative information on blood flow. METHODS: We recorded ICG fluorescence curves of cortex and cerebral vessels using software integrated into the surgical microscop

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18

O’Leary, J. G., H. Kaneku, B. M. Susskind, et al. "High Mean Fluorescence Intensity Donor-Specific Anti-HLA Antibodies Associated With Chronic Rejection Postliver Transplant." American Journal of Transplantation 11, no. 9 (2011): 1868–76. http://dx.doi.org/10.1111/j.1600-6143.2011.03593.x.

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19

Grajek, Hanna, Jacek Kubicki, Ignacy Gryczyński, et al. "Effect of Dimer Structure and Inhomogeneous Broadening of Energy Levels on the Action of Flavomononucleotide in Rigid Polyvinyl Alcohol Films." International Journal of Molecular Sciences 22, no. 14 (2021): 7759. http://dx.doi.org/10.3390/ijms22147759.

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The results of time-resolved fluorescence measurements of flavin mononucleotide (FMN) in rigid polyvinyl alcohol films (PVA) demonstrate that fluorescence intensity decays are strongly accelerated in the presence of fluorescent dimers and nonradiative energy transfer processes. The fluorescence decay originating both from H and J dimer states of FMN was experimentally observed for the first time. The mean fluorescence lifetimes for FMN dimers were obtained: τfl = 2.66 ns (at λexc = 445 nm) and τfl = 2.02 (at λexc = 487 nm) at λobs = 600 nm and T = 253 K from H and J state of dimers, respective

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20

Swatland, H. J. "Optical properties of turkey pectoralis muscle affecting detection of connective tissue fluorescence." Canadian Journal of Animal Science 81, no. 1 (2001): 25–31. http://dx.doi.org/10.4141/a00-053.

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A dual-channel probe was used to detect fluorescence (excitation 365 nm, emission 400 to 550 nm) and reflectance (550 nm) in turkey pectoralis muscles (n= 157). All fluorescence peaks had matching reflectance peaks, but not vice versa. Thus, some reflectance peaks originated from non-fluorescent structures in the muscle. Electrical impedance was used to assess fluid distribution. Electrical capacitance was correlated (P< 0.01) with areas under probe signals (r= 0.19 for both fluorescence and reflectance). In a subset of samples (n= 45), the reflectance of initially polarized light was used

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21

Miller, William G., Maria T. Brandl, Beatriz Quiñones, and Steven E. Lindow. "Biological Sensor for Sucrose Availability: Relative Sensitivities of Various Reporter Genes." Applied and Environmental Microbiology 67, no. 3 (2001): 1308–17. http://dx.doi.org/10.1128/aem.67.3.1308-1317.2001.

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ABSTRACT A set of three sucrose-regulated transcriptional fusions was constructed. Fusions p61RYTIR, p61RYlac, and p61RYice contain thescrR sucrose repressor gene and the promoterlessgfp, lacZ, and inaZreporter genes, respectively, fused to the scrY promoter from Salmonella enterica serovar Typhimurium. Cells ofErwinia herbicola containing these fusions are induced only in media amended with sucrose, fructose, or sorbose. While a large variation in sucrose-dependent reporter gene activity was observed in cells harboring all gene fusions, fusions to theinaZ reporter gene yielded a much wider ra

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22

Kostur, Carrera, Luz Stamm, Lee Ann Tibbles, Kevin McLaughlin, Wenjie Wang, and Noureddine Berka. "95-P: Post transplant monitoring of mean fluorescence intensity (MFI) for high risk renal transplant patients." Human Immunology 70 (November 2009): S59. http://dx.doi.org/10.1016/j.humimm.2009.09.128.

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23

Luiz Costa e Silva, André, Livia Medeiros Soares Celani, Italo Medeiros Azevedo, and Aldo Cunha Medeiros. "Levamisole in treatment of urethane-induced pulmonary carcinoma in rats." JOURNAL OF SURGICAL AND CLINICAL RESEARCH 10, no. 2 (2019): 54–64. http://dx.doi.org/10.20398/jscr.v10i2.18782.

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Objective: The potential antitumor effects of the levamisole immunomodulatory agent remain uncertain, and its beneficial effects with increased survival in the adjuvant treatment of malignant tumors are controversial. The present study aims to compare the effects of levamisole with cisplatin in the treatment of urethane-induced lung carcinoma in rats. Methods: Wistar rats were allocated into three groups (n = six each). Group A: mice with lung tumor + treatment with levamisole. Group B: mice with lung tumor + cisplatin treatment. Group C: mice with lung tumor + saline treatment. After 12 weeks

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24

Wheeler, Emily, Trish Berger, and Esmail Behboodi. "Bovine oocyte plasma membrane binding sites for sperm plasma membrane during in vitro oocyte maturation and fertilisation." Zygote 4, no. 1 (1996): 67–72. http://dx.doi.org/10.1017/s0967199400002902.

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SummaryThe experimental objective was to determine whether the capability of bovine oocyte plasma membrane to bind sperm changes during in vitro oocyte maturation and fertilisation. Binding was quantified by the intensity of tetramethylrhodamine isothiocyanate (TRITC) fluorescence at the periphery of oocytes following incubation with biotinylated sperm plasma membrane proteins and subsequent incubation with TRITC-avidin. Bovine oocytes were matured in vitro. Sample groups were removed after 0,6 and 22 h, or inseminated and further cultured for 24 or 48 h. Oocytes were denuded of cumulus cells

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25

Guibert, Philippe, William Perrard, and Ce´line Morin. "Concentration Measurements in a Pressurized and Heated Gas Mixture Flow Using Laser Induced Fluorescence." Journal of Fluids Engineering 124, no. 2 (2002): 512–22. http://dx.doi.org/10.1115/1.1456462.

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The fuel concentration in a pressurized and heated gas mixture flow was measured by LIF (laser induced fluorescence) technique. Diacetyl was used as the fluorescence tracer of fuel and was excited at a wavelength of 355 nm. Influent parameters on the LIF intensity among the equivalence ratio, the environment temperature and pressure, the flow velocity were determined from a parametric study. The technique of plans of experiments with statistical tests and analysis was investigated to determine exactly the preponderant parameters and their influence on the LIF intensity. For the experimental co

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26

Benammar Elgaaied, Amel, Donato Cascio, Salvatore Bruno, et al. "Computer-Assisted Classification Patterns in Autoimmune Diagnostics: The AIDA Project." BioMed Research International 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/2073076.

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Antinuclear antibodies (ANAs) are significant biomarkers in the diagnosis of autoimmune diseases in humans, done by mean of Indirect ImmunoFluorescence (IIF) method, and performed by analyzing patterns and fluorescence intensity. This paper introduces the AIDA Project (autoimmunity: diagnosis assisted by computer) developed in the framework of an Italy-Tunisia cross-border cooperation and its preliminary results. A database of interpreted IIF images is being collected through the exchange of images and double reporting and a Gold Standard database, containing around 1000 double reported images

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27

Stosik, M., W. Deptula, K. Wiktorowicz, M. Trávníček, and K. Baldy-Chudzik. "Respiratory burst in neutrophilic granulocytes of carps (Cyprinus carpio): cytometric studies." Veterinární Medicína 47, No. 1 (2012): 17–20. http://dx.doi.org/10.17221/5797-vetmed.

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Present studies aimed at flow cytometric analysis of respiratory burst in neutrophilic granulocytes in carps, at various stages of their ontogeny. Cytometric evaluation of the repiratory burst in PMN cells of carps demonstrated augmented values of the mean fluorescence channel of PMA-stimulated PMN cells, as compared to the value of the fluorescence channel noted for PMN cells which were not activated or did not respond to activation in response to the applied stimulator. Intensity of the fluorescence, compared to the mean fluorescence channel of the cells which were not stimulated by PMA, was

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28

Kaneku, H., J. G. O’Leary, B. M. Susskind, L. W. Jennings, G. B. Klintmalm, and P. I. Terasaki. "High Mean Fluorescence Intensity DSA and IgG3 Subclass DSA after Liver Transplantation Decrease Patient and Graft Survival." Transplantation Journal 94, no. 10S (2012): 324. http://dx.doi.org/10.1097/00007890-201211271-00602.

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29

Domen, Ronald E., Heather Casey, and Justine Gaspari. "56-P: Correlation of HLA donor specific antibody (DSA) titers with the luminex mean fluorescence intensity (MFI)." Human Immunology 69 (October 2008): S35. http://dx.doi.org/10.1016/j.humimm.2008.08.075.

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30

Oh, E., H. S. Kim, and M. Yang. "Correlation of HLA antibody mean fluorescence intensity with C1q binding status in EDTA treated real clinical samples." Clinica Chimica Acta 493 (June 2019): S63. http://dx.doi.org/10.1016/j.cca.2019.03.140.

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31

Suzuki, Tomonari, Satoru Wada, Hidetaka Eguchi, et al. "Cadherin 13 overexpression as an important factor related to the absence of tumor fluorescence in 5-aminolevulinic acid–guided resection of glioma." Journal of Neurosurgery 119, no. 5 (2013): 1331–39. http://dx.doi.org/10.3171/2013.7.jns122340.

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Object Gliomas contain aggressive malignant cancer, and resection rate remains an important factor in treatment. Currently, fluorescence-guided resection using orally administered 5-aminolevulinic acid (5-ALA) has proved to be beneficial in improving the prognosis of patients with gliomas. 5-ALA is metabolized to protoporphyrin IX (PpIX) that accumulates selectively in the tumor and exhibits strong fluorescence upon excitation, but glioma cells do not always respond to 5-ALA, which can result in incomplete or excessive resection. Several possible mechanisms for this phenomenon have been sugges

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32

Wang, Shuhang, Wenwen Wang, Junyi Chen, Bo Zhang, Li Zhao, and Xia Jiang. "Characteristics of Dissolved Organic Matter and Its Role in Lake Eutrophication at the Early Stage of Algal Blooms—A Case Study of Lake Taihu, China." Water 12, no. 8 (2020): 2278. http://dx.doi.org/10.3390/w12082278.

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Decaying algal blooms in eutrophic lakes can introduce organic matter into the water and change nutrient concentrations in the water column. The spatial distribution and composition characteristics, sources, and contribution to eutrophication of dissolved organic matter (DOM) in the overlying water of Lake Taihu, a typical eutrophic lake in China, were analyzed by ultraviolet–visible spectra and three-dimensional fluorescence excitation–emission matrix spectra combined with the statistical decomposition technique, parallel factor analysis. The concentration of DOM was represented by dissolved

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33

El-Sherbiny, Dina T. "Spectrofluorometric Determination of Citalopram in Pharmaceutical Preparations and Spiked Human Plasma Using Organized Media." Journal of AOAC INTERNATIONAL 89, no. 5 (2006): 1288–95. http://dx.doi.org/10.1093/jaoac/89.5.1288.

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Abstract The native fluorescence of citalopram (CIT) was (obtained in citrate buffer of pH 6.5 with and without β-cyclodextrin (β-CD) or sodium dodecyl sulfate (SDS) as fluorescence enhancers at 305 nm using 242 nm for excitation. Micellar systems of ionic and nonionic surfactants were investigated by measuring the fluorescence intensity of the analyte-surfactant system. In slightly acidic aqueous solution of pH 6.5, CIT was better incorporated in CDs and SDS micelles. The luminescence emission from CIT was found to be greatly enhanced by SDS micelles. The fluorescence intensity enhancements i

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34

Giovannacci, Ilaria, Marco Meleti, Federico Garbarino, et al. "Correlation between Autofluorescence Intensity and Histopathological Features in Non-Melanoma Skin Cancer: An Ex Vivo Study." Cancers 13, no. 16 (2021): 3974. http://dx.doi.org/10.3390/cancers13163974.

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Non-melanoma skin cancer (NMSC) is the most common malignant tumor affecting fair-skinned people. Increasing incidence rates of NMSC have been reported worldwide, which is an important challenge in terms of public health management. Surgical excision with pre-operatively identified margins is one of the most common and effective treatment strategies. Incomplete tumor removal is associated with a very high risk of recurrence and re-excision. Biological tissues can absorb and re-emit specific light wave-lengths, detectable through spectrophotometric devices. Such a phenomenon is known as autoflu

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35

Markowitz, K., and K. Carey. "Assessing the Appearance and Fluorescence of Resin-Infiltrated White Spot Lesions With Caries Detection Devices." Operative Dentistry 43, no. 1 (2018): E10—E18. http://dx.doi.org/10.2341/16-153-l.

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SUMMARY Objective: This in vitro study examined the effectiveness of caries detector devices in assessing the ability of resin infiltration (RI) (Icon, DMG-Hamburg, Hamburg, Germany) to improve the optical properties of enamel white spot lesions (WSLs). Methods and Materials: Ten caries-free third molars were used. Photographs, a subjective visual assessment of the photographs, fluorescent camera (FC) images using the Spectra (Air Techniques, Melville, NY, USA), and laser fluorescent (LF) readings using the DIAGNOdent (KaVo, Biberach, Germany) were obtained from each tooth's buccal surface. Sp

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36

Yates, Ronald L., and John A. Wenninger. "Fluorometric Determination of Benzylideneacetone in Fragrance Products by Liquid Chromatography with Post-Column Derivatization." Journal of AOAC INTERNATIONAL 71, no. 5 (1988): 965–67. http://dx.doi.org/10.1093/jaoac/71.5.965.

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Abstract A method is described for the liquid chromatographic (LC)-fluorometric determination of benzylideneacetone in fragrance products. Benzylideneacetone is first separated from other fragrance ingredients by LC and then reacted post-column with a methanolic solution of isonicotinic acid hydrazide and aluminum nitrate. The reactants are maintained at 65°C for about 1.5 min to quantitatively form the fluorescent isonicotinoyl hydrazone derivative of benzylideneacetone. The aluminum ion forms a complex with the hydrazone to enhance the fluorescence of the derivative. The amount of benzyliden

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37

Schimmel, Carmel, David Frazer, and Robb W. Glenny. "Extending fluorescent microsphere methods for regional organ blood flow to 13 simultaneous colors." American Journal of Physiology-Heart and Circulatory Physiology 280, no. 6 (2001): H2496—H2506. http://dx.doi.org/10.1152/ajpheart.2001.280.6.h2496.

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Seven fluorescent microsphere colors can be used in a single experiment to estimate regional blood flow without correcting for spillover of emitted fluorescence. To extend the method to 13 colors, we compared the accuracy of three methods for spillover correction. Fixed wavelength intensities were corrected by matrix inversion, and synchronous scan spectra were corrected by least squares fit of an overdetermined system of linear equations and by least squares fit of a sum of Gaussian and Lorentzian functions. Correction methods were validated in pigs and sheep by simultaneous injections of rad

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38

Chuksina, J. J., E. V. Kataeva, and T. A. Mitina. "Features of immunophenotypic finding B-cell lymphoproliferative diseases by flow cytometry." Kazan medical journal 101, no. 1 (2020): 145–52. http://dx.doi.org/10.17816/kmj2020-145.

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Aim. To assess the information content of conventional and additional immunophenotypic markers (CD200, CD305) in the differential diagnosis B-cell lymphoproliferative diseases by flow cytometry. Methods. An immunophenotypic study using 4-color flow cytometry was performed in 204 patients with different variants of B-cell non-Hodgkin's lymphomas. The study material included peripheral blood and bone marrow. The expression of CD45, CD19, CD20, CD22, CD79b, CD79a, CD5, CD10, CD23, FMC7, CD43, CD38, CD11c, CD103, CD25, CD 200, CD 305, light chains of immunoglobulins (kappa/lambda) using mono

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39

Wells, Denise A., and Michael R. Loken. "Erratum to “Flow cytometric mean fluorescence intensity: The biophysics behind the number” [Leukemia Res. 32 (2008) 845–846]." Leukemia Research 32, no. 11 (2008): 1792. http://dx.doi.org/10.1016/j.leukres.2008.06.021.

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40

Gloudemans, A. J., E. Kuulkers, R. Campana, A. Escalante, M. Kole, and Y. Mollard. "Re-evaluation of Lunar X-ray observations by Apollo 15 and 16." Astronomy & Astrophysics 649 (May 2021): A174. http://dx.doi.org/10.1051/0004-6361/202140321.

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The Apollo 15 and 16 missions were the first to explore the Lunar surface chemistry by investigating about 10% of the Lunar surface using a remote sensing X-ray fluorescence spectrometer experiment. The data obtained have been extensively used to study Lunar formation history and geological evolution. In this work, a re-evaluation of the Apollo 15 and 16 X-ray fluorescence experiment is conducted with the aim of obtaining up-to-date empirical values for aluminum (Al) and magnesium (Mg) concentrations relative to silicon (Si) of the upper Lunar surface. An updated instrument response, a newly r

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41

Zhao, Jin Hui, Hai Bin Xiao, Hai Chao Yuan, Qian Hong, and Mu Hua Liu. "Application of Three-Dimensional Fluorescence Spectroscopy Coupled with ATLD in Rapid Determination of Triazophos Content in Duck Meat." Applied Mechanics and Materials 651-653 (September 2014): 362–66. http://dx.doi.org/10.4028/www.scientific.net/amm.651-653.362.

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The triazophos is a kind of organic phosphorus pesticide, the quantitative determination model based on three-dimensional fluorescence spectroscopy coupled with alternating trilinear decomposition (ATLD) was explored for realizing the rapid determination of triazophos content in duck meat. Firstly, three-dimensional fluorescence spectra of duck meat extract, triazophos standard solution and duck meat extract containing triazophos were explored, respectively. Secondly, the fluorescence quenching phenomenon of the triazophos in duck meat extract was analyzed. Lastly, the number of components for

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Murase, N., M. Y. Gao, N. Gaponik, T. Yazawa, and J. Feldmann. "SYNTHESIS AND OPTICAL PROPERTIES OF WATER SOLUBLE ZnSe NANOCRYSTALS." International Journal of Modern Physics B 15, no. 28n30 (2001): 3881–84. http://dx.doi.org/10.1142/s0217979201008901.

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ZnSe nanocrystals are prepared in water by a wet chemistry method. By selecting an appropriate pH value and surface-capping agents, a whitish blue fluorescence peaking at 470 nm is observed under ZV irradiation. The intensity of this fluorescence increases dramatically under reflux and saturates after ~ 40 hrs. The final mean size of the ZnSe nanocrystals measured by transmission electron microscopy is aboyt 2 nm in diameter. The quantum efficiency of the fluorescence form the final solution is estimated to be ~1%, although the preparation conditions have not yet been completely optimized. The

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McLennan, H. J., M. L. Sutton-McDowall, S. Heng, and J. G. Thompson. "153 Fertilisation of Cattle Oocytes is Linked to Novel Waves of a Ca2+ Fluorophore in Cumulus Cells." Reproduction, Fertility and Development 30, no. 1 (2018): 216. http://dx.doi.org/10.1071/rdv30n1ab153.

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During fertilization, multiple intracellular calcium (Ca2+) oscillations are initiated after sperm binding to the oocyte vitelline membrane. This Ca2+ signalling has been extensively studied in denuded mouse and Xenopus oocytes but minimally studied in larger mammals. Cows in particular are unusual, as the few studies on oocyte activation have observed fewer Ca2+ oscillations during fertilisation compared with mice. Furthermore, cattle intracytoplasmic sperm injection (ICSI) is inefficient, despite parthenogenetic activation occurring readily. We hypothesise that cumulus cells are important fo

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Dendrou, Calliope A., Erik Fung, Laura Esposito, John A. Todd, Linda S. Wicker, and Vincent Plagnol. "Fluorescence Intensity Normalisation: Correcting for Time Effects in Large-Scale Flow Cytometric Analysis." Advances in Bioinformatics 2009 (November 17, 2009): 1–6. http://dx.doi.org/10.1155/2009/476106.

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A next step to interpret the findings generated by genome-wide association studies is to associate molecular quantitative traits with disease-associated alleles. To this end, researchers are linking disease risk alleles with gene expression quantitative trait loci (eQTL). However, gene expression at the mRNA level is only an intermediate trait and flow cytometry analysis can provide more downstream and biologically valuable protein level information in multiple cell subsets simultaneously using freshly obtained samples. Because the throughput of flow cytometry is currently limited, experiments

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Gebhard, D. F., A. Mittelman, C. Cirrincione, H. T. Thaler, and B. Koziner. "Comparative analysis of surface membrane immunoglobulin determination by flow cytometry and fluorescence microscopy." Journal of Histochemistry & Cytochemistry 34, no. 4 (1986): 475–81. http://dx.doi.org/10.1177/34.4.3081624.

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The analysis of membrane surface immunoglobulin (SmIg) on B lymphocytes was carried out in 59 normal individuals and nine patients with B-cell non-Hodgkin's lymphomas by conventional immunofluorescence microscopy and flow cytometry. Five channel settings of a cytofluorograph were evaluated (100, 150, 200, 250, 300) and the mean and standard deviation of the percent positive cells were calculated and compared to the mean and standard deviation of the microscope reading. On the basis of the relative fluorescence reactivity, we were able to determine a fluorescence intensity at which the results

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Narumi, Shunji, Yoshihiko Watarai, Norihiko Goto, et al. "Everolimus-based Immunosuppression Possibly Suppresses Mean Fluorescence Intensity Values of De Novo Donor-specific Antibodies After Primary Kidney Transplantation." Transplantation Proceedings 51, no. 5 (2019): 1378–81. http://dx.doi.org/10.1016/j.transproceed.2019.03.019.

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Liu, Lin-I., Nikolay N. Barashkov, Tamara S. Novikova, Chintamani P. Palsule, Shubhra Gangopadhyay, and Walter L. Borst. "Fluorescence Studies on New Epoxypolymer—Dye Compositions for Ultrafast Wavelength Shifters." Applied Spectroscopy 50, no. 12 (1996): 1545–52. http://dx.doi.org/10.1366/0003702963904467.

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Fast wavelength shifters with fluorescence decay times in the low nanosecond range were prepared from mixtures of various dyes in epoxypolymer compositions on the base of diglycidyl ether of bisphenol-A (DGEBA) with hexahydrophthalic anhydride (HHPA) or trimethoxyboroxine (TMBOX). Three possible sample preparation methods yielding high transmittance and high-fluorescence intensity were compared. The characteristics of the new wavelength shifters were determined with continuous-wave (cw) excitation of the fluorescence and by pulsed-laser excitation, the latter coupled with an analysis of the fl

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Abedi, Mehrdad, Deborah A. Greer, Bethany A. Foster, et al. "Marrow Derived Muscle Colonies Are a Clonal Phenomenon." Blood 104, no. 11 (2004): 2692. http://dx.doi.org/10.1182/blood.v104.11.2692.2692.

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Abstract We have previously established a model of plasticity of marrow to skeletal muscle that is based on transplantation of GFP positive marrow and cardiotoxin injury to the tibialis muscle. In these experiments, we have observed colonies of GFP positive muscle fibers, defined as 3 or more myocytes. We noted that individual GFP positive fibers showed marked heterogeneity of fluorescence. In sharp contrast, colonies of GFP+ cells, showed homogeneous fluorescence. This was confirmed by using confocal microscopy and by measuring the mean fluorescent intensity of the individual cells in the col

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Chen, Sisi, Jianing Wang, Lei Zhang, and Hao Xia. "Experimental study on alleviating atherosclerosis through intervention of mitochondrial calcium transport and calcium-induced membrane permeability transition." Journal of Investigative Medicine 69, no. 6 (2021): 1156–60. http://dx.doi.org/10.1136/jim-2020-001765.

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To investigate the effort of mitochondrial calcium transport and calcium-induced membrane permeability transition in alleviating atherosclerosis. The experimental mice were divided into three groups: the control group (C57BL/6 mice with normal diet), the atherosclerosis group (apolipoprotein E-deficient (ApoE−/−) mice with high-fat diet) and the mitochondrial targeting agent group (ApoE−/− mouse with high-fat diet). The mean fluorescence intensity of Ca2+ in the atherosclerosis group is significantly higher than control group and mitochondrial targeting agent group. But the mean fluorescence i

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Borowitz, Michael J., Jonathan Shuster, Andrew J. Carroll, et al. "Prognostic Significance of Fluorescence Intensity of Surface Marker Expression in Childhood B-Precursor Acute Lymphoblastic Leukemia. A Pediatric Oncology Group Study." Blood 89, no. 11 (1997): 3960–66. http://dx.doi.org/10.1182/blood.v89.11.3960.

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Abstract This report describes the prognostic significance of the intensity of surface membrane antigen expression in a series of 1,231 children older than 1 year with newly diagnosed B-precursor acute lymphoblastic leukemia (ALL) treated on Pediatric Oncology Group (POG) treatment protocols. All patients had dual-color flow cytometric immunophenotyping performed at a central reference laboratory with a standard panel of monoclonal antibodies. The flow cytometers used in the study were calibrated with a standard fluorescence microparticle that permitted conversion of relative fluorescence chan

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