Academic literature on the topic 'Measuring Tritop system'

The paper presents the results of measuring the gearbox housing with the ATOS optical measuring system, the TRITOP system and the "TESA micro-hit 4-5-4" coordinate measuring machine. The aim of the study was to determine the differences in modern measuring systems and whether these differences affect the final measurement result when controlling parts of a complex configuration. At the beginning of the paper, the way of functioning of the used measuring systems and the results of research in this area are described. GOM Inspect software used for the ATOS and TRITOP measuring systems, while PC DMIS software used for the coordinate measuring machine. The analysis of the obtained results showed that there are significant differences in the measurement results and that the measuring systems used can't be applied with the same success to the measurement of parts of complex configuration such as the gearbox housing.

In the paper the modern measurement methods of structural deformation using GOM optical measuring systems based on digital image processing were presented. First part of the work demonstrates the results of measurement of initial geometrical imperfections of steel structural elements using the Tritop optical 3D coordinate measuring machine. The method of preparation of sigma type steel beams with a height of 200 mm, a flange width of 70 mm and a wall thickness of 2 mm is presented. Afterwards, the subsequent stages of measurements and the method of reading the obtained results and their interpretation are described. The next step presents a test stand designed to perform strength tests of thin-walled sigma beams in a four-point bending scheme. In addition, the method of preparation of beam’s surface to measurement of 3D using the Aramis system and electrofusion strain gauges is presented. The deformation values obtained for three sigma-type steel beams were compared with respect of two mentioned measurement methods. The final conclusions and recommendations regarding the methodology of geometry and strain measurement using optical measuring systems complete the work.

A handheld 3D laser scanning system is proposed for measuring large-sized objects on site. This system is mainly composed of two CCD cameras and a line laser projector, in which the two CCD cameras constitute a binocular stereo vision system to locate the scanner’s position in the fixed workpiece coordinate system online, meanwhile the left CCD camera and the laser line projector constitute a structured light system to get the laser lines modulated by the workpiece features. The marked points and laser line are both obtained in the coordinate system of the left camera in each moment. To get the workpiece outline, the handheld scanner’s position is evaluated online by matching up the marked points got by the binocular stereo vision system and those in the workpiece coordinate system measured by a TRITOP system beforehand; then the laser line with workpiece’s features got at this moment is transformed into the fixed workpiece coordinate system. Finally, the 3D information composed by the laser lines can be reconstructed in the workpiece coordinate system. A ball arm with two standard balls, which is placed on a glass plate with many marked points randomly stuck on, is measured to test the system accuracy. The distance errors between the two balls are within ±0.05 mm, the radius errors of the two balls are all within ±0.04 mm, the distance errors from the scatter points to the fitted sphere are distributed evenly, within ±0.25 mm, without accumulated errors. Measurement results of two typical workpieces show that the system can measure large-sized objects completely with acceptable accuracy and have the advantage of avoiding some deficiencies, such as sheltering and limited measuring range.

Cadmium is one of the transition metals, known by the scientific name Cd. One of its main characteristics is the high toxicity, even in very little amounts. Cadmium is often released through industrial effluents, pesticides, chemical fertilizers, and the burning of fossil fuels. Since the presence of cadmium ions in the living organisms’ body, especially humans, can cause serious damage to the liver and pancreas, and also because its role in causing cancer has been proven, measuring very low amounts of this metal is of high importance. In the first step, this study has reviewed and analyzed common laboratory methods for measuring small amounts of cadmium. Then, according to economic, environmental, feasibility, speed, and accuracy factors, all available methods were evaluated using the ELECTRE technique. The results showed that the extraction methods using Dowex Optipore V-493 resin and extraction system in Triton X-114 surfactant, placed in the first and second positions.

Introduction : Real-time monitoring of blood loss is critical in fluid management. Visual estimation remains the standard of care in estimating blood loss, yet is demonstrably inaccurate. Photometric analysis, which is the referenced “gold-standard” for measuring blood loss, is both time-consuming and costly. The purpose of this study was to evaluate the efficacy of a novel tablet-monitoring device for measurement of Hb loss during orthopaedic procedures. Methods : This is a prospective study of 50 patients in a consecutive series of joint arthroplasty cases. The novel System with Feature Extraction Technology was used to measure the amount of Hb contained within surgical sponges intra-operatively. The system’s measures were then compared with those obtained via gravimetric method and photometric analysis. Accuracy was evaluated using linear regression and Bland-Altman analysis. Results : Our results showed a significant positive correlation between Triton tablet system and photometric analysis with respect to intra-operative hemoglobin and blood loss at 0.92 and 0.91, respectively. Discussion : This novel system can accurately determine Hb loss contained within surgical sponges. We believe that this user-friendly software can be used for measurement of total intraoperative blood loss and thus aid in a more accurate fluid management protocols during orthopaedic surgical procedures.

AbstractNitric oxide (NO) is known to be synthesized in several tissues and to increase the formation of cyclic GMP through the activation of soluble guanylate cyclases. Since cyclic GMP plays an important role in visual transduction, we investigated the presence of nitric oxide synthesizing activity in retinal rod outer segments. Bovine rod outer segments were isolated intact and separated into membrane and cytosolic fractions. Nitric oxide synthase activity was assayed by measuring the conversion of L-arginine to L-citrulline. Both membrane and cytosolic fractions were active in the presence of calcium and calmodulin. The activity in both fractions was stimulated by the nitric oxide synthase cofactors FAD, FMN, and tetrahydrobiopterin and inhibited by the L-arginine analog, L-monomethyl arginine. The Km for L-arginine was similar, about 5 μM for the enzyme in both fractions. However, the two fractions differed in their calcium/calmodulin dependence: the membrane fraction exhibited basal activity even in the absence of added calcium and calmodulin while the cytosolic fraction was inactive. But the activity increased in both fractions when supplemented with calcium/calmodulin: in membranes from about 40 to 110 fmol/min/mg of protein and in the cytosol from near zero to about 350 fmol/min/mg of protein in assays carried out at 0.3 μM L-arginine. The two enzymes also responded differently to detergent: the activity of the membrane enzyme was doubled by Triton X-100 while that of the cytosolic enzyme was unaffected. These results show that NO is produced by cytosolic and membrane-associated enzymes with distinguishable properties. Investigations on the purity of isolated ROS showed that about 50% of the NOS activity is endogenous to the outer segments, and that the rest is due to membrane vesicles rich in Na, K-ATPase activity. If and how NO influences the rod outer segment physiology remains to be investigated.

Studies of rats have indicated that skeletal muscle plays a central role in whole-body nitrate ([Formula: see text])/nitrite ([Formula: see text])/nitric oxide (NO) metabolism. Extending these results to humans, however, is challenging due to the small size of needle biopsy samples. We therefore developed a method to precisely and accurately quantify [Formula: see text] and [Formula: see text] in biopsy-sized muscle samples. [Formula: see text] and [Formula: see text] were extracted from rat soleus samples using methanol combined with mechanical homogenization + ultrasound, bead beating, pulverization at liquid N2temperature or pulverization + 0.5% Triton X-100. After centrifugation to remove proteins, [Formula: see text] and [Formula: see text] were measured using HPLC. Mechanical homogenization + ultrasound resulted in the lowest [Formula: see text] content (62 ± 20 pmol/mg), with high variability [coefficient of variation (CV) >50%] across samples from the same muscle. The [Formula: see text]/[Formula: see text] ratio (0.019 ± 0.006) was also elevated, suggestive of [Formula: see text] reduction during tissue processing. Bead beating or pulverization yielded lower [Formula: see text] and slightly higher [Formula: see text] levels, but reproducibility was still poor. Pulverization + 0.5% Triton X-100 provided the highest [Formula: see text] content (124 ± 12 pmol/mg) and lowest [Formula: see text]/[Formula: see text] ratio (0.008 ± 0.001), with the least variability between duplicate samples (CV ~15%). These values are consistent with literature data from larger rat muscle samples analyzed using chemiluminescence. Samples were stable for at least 5 wk at −80°C, provided residual xanthine oxidoreductase activity was blocked using 0.1 mmol/l oxypurinol. We have developed a method capable of measuring [Formula: see text] and [Formula: see text] in <1 mg of muscle. This method should prove highly useful in investigating the role of skeletal muscle in [Formula: see text]/[Formula: see text]/NO metabolism in human health and disease.NEW & NOTEWORTHY Measurement of nitrate and especially nitrite in small, i.e., biopsy-sized, muscle samples is analytically challenging. We have developed a precise, accurate, and convenient method for doing so using an affordable commercial HPLC system.

Techniques for measuring ATP bioluminescence are being used widely as rapid methods for the assessment of the cleanliness of food-processing plants. Sanitizer or cleanser residues could present a potential problem in the use of these ATP bioluminescence techniques due to the degradation of the firefly luciferin-luciferase substrate-enzyme system by these cleaning chemicals. The objectives of this study were the evaluation of the quenching and enhancement effects on the detection of the ATP bioluminescence signal using various ATP extractants, commercial cleansers, and sanitizers, and the determination of the antimicrobial properties of different concentrations of cleansers and sanitizers on Escherichia coli OI57:H7, Listeria monocytogenes, Staphylococcus aureus, and Pseudomonasfragi. Extractants evaluated were benzalkonium chloride, Triton X-100,benzethonium chloride, cetylpyridinium chloride, and trichloroacetic acid. Cleansers evaluated were an alkaline foam and an acid foam. Also evaluated were a quaternary ammonium sanitizer, a d-limolene sanitizer, commercial sodium hypochlorite, and household bleach (sodium hypochlorite). The extractant cetylpyridinium chloride (0.0125%) did not have a statistically significant effect on the detection of the ATP bioluminescence signal at a 95% confidence level. A transition from enhancement to quenching as a concentration-dependent phenomenon was observed for the alkaline foam, acid foam, commercial sodiumhypochlorite,d-limolene,and household bleach. An enhancement effect that did not appear to be concentration-dependent was observed for the quaternary ammonium sanitizer. Antimicrobial disc assays demonstrated that in some cases the cleanser or sanitizer concentration was not effective against the bacteria, but enhanced or quenched the detection of the bioluminescence signal, leading to false-positive or false-negative results respectively.

OBJECTIVES/SPECIFIC AIMS: (1) To delineate the function of the heavy-chain receptor binding domain (HCR), a portion of botulinum neurotoxin type A (BoNT/A) and synaptic vesicle 2 (SV2) signaling pathway, which provide a novel multipurpose biologic with potential clinical applications in tumor detection/imaging, inhibition of tumor progression, and reduction of bioactive hormone secretion in metastatic neuroendocrine (NE) cancers. (2) To evaluate the expression pattern of SV2 receptors in NE cancer patient-derived tissues for prediction of patient response for recombinant HCR (rHCR) treatment. (3) To assess the in vivo efficacy and toxicity of rHCR in a NE cancer liver metastasis mouse models and in the NE patient-derived 3D MicroTumor system. (4) To collect preclinical data to design and conduct a clinical trial with NE cancer patients, a major goal toward translating our discoveries into much needed therapies. METHODS/STUDY POPULATION: Recombinant botulinum heavy chain (rHCR) was produced using an IPTG-inducible expression vector in E. coli BL21. The rHCR was His-Tag purified and stored in PBS buffer before usage. Cytotoxicity: H727, TT, and MZ cells were plated at a density of 5000 cells/well in 96-well plates and incubated under standard conditions overnight. The next day, cells were treated with 10, 100, or 500 nmol/L of rHCR and incubated for 72 hours. Following incubation, cell viability was assessed by ATP quantification using the CellTiter-Glo (Promega) assay. Fresh NE tumors were dissociated and injected into polydimethylsiloxane bioreactors in a matrigel and collagen suspension for 3D culture experiments. The viability of 3D cultures incubated with various doses of rHCR was assessed by measuring the uptake of the near-infrared dye IR-783 using an IVIS imaging system. Western blot: H727, TT, and MZ cells were seeded in 6-well plates at a density of 3×105 cells/well for 24 hours followed by treatment with 100 nmol/L for 72 hours. Total cellular proteins were isolated and analyzed to assess the level of SV2A expression and the effect of rHCR on the expression levels of NET marker proteins. Immunohistochemistry: Deparaffinized tissue culture slides were incubated with SV2A primary antibody in 1% BSA and incubated overnight at 4°C. Slides were rinsed twice with TBS containing 0.025% Triton, followed by 0.3% H2O2 for 15 minutes. Slides were then incubated with HRP-conjugated secondary antibody for 1 hour at room temperature. Detection of protein-protein interaction: Precleared cell lysate was incubated with glutathione-agarose beads in the presence of 10 μg of GST-tagged rHCR for 2 h at 4°C with end-over end mixing. Samples were then centrifuged at 10,000 g for 2 minutes and the supernatant was analyzed by SDS-PAGE. Preclinical models: To allow rHCR testing on NET patient derived cells in a very novel 3D surrogates, sterile collected fresh NET tissues will be obtained from the UAB Tissue Procurement, dissociated into a single cell suspension and injected into a polydimethylsiloxane bioreactors containing extracellular matrix composed of bovine collagen and matrigel. Such 3D cell culture will be maintained in bioreactors with constant supply of media through the microchannels and treated with rHCR at concentrations ranging from 10 nM to 1 mM. Following histologic confirmation of growth and morphology of NET patient-derived 3D surrogates we will test the anticancer activity of rHCR in this system using the standard cytotoxicity assays as well as we will validate the NET hormone expression using immunohistochemistry assay. To create an animal model of NE cancer progression, we will perform intrasplenic injection of NET cell lines. In approximately 4 weeks, the animals should develop NE liver metastases based upon our previous experience. rHCR-iFPs accumulation in the tumor mass: rHCR-iFPs will be injected to the tumor bearing mice after 4 weeks of cells implantation in 1 week interval for total of 4 treatments at the concentrations of 0.125, 1.25, and 12.5 mg/kg. RESULTS/ANTICIPATED RESULTS: Based on the preliminary data, we expect to detect rHCR-iFPs in NE cancer xenografts and in patient derived 3D explants. Our preliminary data revealed that treating NETcells with rHCR significantly reduced NE peptide expression in 3 days. Thus, we expect to see the decrease of NE tumor markers even if the fluorescent detection method is not sensitive enough to monitor the signal. The reduction of the NET markers can be used as an indicator of the rHCR-iFPs uptake by the tumor mass. If HCR exhibit high binding affinity to SV2 receptors in NET models but moderate anticancer efficacy, we plan to use rHCR peptide to conjugate with the nanocarrier for targeted drug delivery. In this case rHCR peptide can be used as a ligand that specifically binds to NE cancer cells and delivers anticancer drug. In 3D NET patient derived explants we expect significant reduction of NET markers and hormones (serotonin and calcitonin) in NE cancer cells upon long-term rHCR-iFPs treatment. In addition, we will perform multiplex protein quantification assay using Luminex to assess the various hormones, cytokines, and growth factors to be repurposed into a diagnostic and detection reagent, or a drug delivery ligand for targeted therapies. DISCUSSION/SIGNIFICANCE OF IMPACT: NE cancers are highly metastatic: NE cancers such as carcinoid, islet cell tumors, and medullary thyroid cancer frequently metastasize to the liver. They are the second most prevalent GI malignancy. Ninety percent of patients with pancreatic carcinoid tumors and 50% of patients with islet cell tumors develop isolated hepatic metastases. Patients with untreated, isolated NE liver metastases have <30% 5-year survival. Thus, there is a critical need for new therapies for NE cancers. Surgery is the only curative therapy available for patients with NE cancers but most cannot be cured: surgical removal is the most effective treatment for NE cancers; however, a very high percentage of patients present with metastatic disease. While surgical resection can be potentially curative, many patients are not candidates for operative intervention due to widespread metastases or the degree of hepatic involvement by the NE cancers. Moreover, other forms of therapy including chemoembolization, radioembolization, cryoablation, and chemotherapy have had limited efficacy. We hope that our in vitro data on rHCR toxicity and specificity to NET, validated in the pre-clinical models will allow the first in-human application of this technology in clinical studies.

This work deal with, suggestion of measuring procedure of deformation modulus of system Tritop and his application on real instances. Also the measuring procedure of torsional stiffness of vehicle body is designed, and the models of frames including attachment of a car are elaborated, where the measuring of torsional stiffness will be done on.

This master's thesis deals with measurement of torsional rigidity of a vehicle body with the application of TRITOP optical measuring system. The measurement was made in dynamic test-room of Škoda Auto a.s. together with a methodology used by this company. In the light of the objective comparison of both measuring methods and their accuracy, the optimal method of measurment and evaluation was made.

This thesis deals with the measurement of the torsional stiffness of the frame using Tritop system. There is described development of the frame due to the complexity of torsional stiffness. Further there is outlined a brief overview of the types of measurement and the effect of torsional stiffness on the driving behavior of the vehicle. The aim of the work is to develop a method for measuring torsional stiffness of the vehicle Formula Student and from the results of repeated measurements to assess the accuracy and repeatability of measurements.

In pursuit of improved safety, Norfolk Southern Corp. (NS) has partnered with Amberg Technologies to explore the potential benefits of a laser-based measurement system for measuring over dimensional freight rail shipments. Shipments that do not fall within a standard geometric envelope, denoted as Plate B in the Association of American Railroads (AAR) Open Top Loading Rules [1], are considered to be over dimensional, or High-Wide Loads (HWLs). Extending beyond the limits of the Plate B diagram, these loads are not permitted in unrestricted interchange service. Instead, they must be measured both at points of origin and at interchange points. For US Class I Railroads, the de facto method for measuring HWLs requires mechanical personnel to either climb on the equipment or use a ladder and physically measure the overall height and width of the load. Using a tape measure, plumb line, and 6-foot level, car inspectors, or carmen, must often make multiple measurements to determine the height or width of a critical point on the load. The summation of these measurements can be subject to mathematical human error. In addition to the inherent limitations with regards to accuracy and efficiency, this method of measurement presents considerable safety challenges. The objective of the project was to develop a portable, cost-effective and accurate measurement system to improve the day-to-day operational process of measuring HWLs and reduce human exposure to railyard hazards. Norfolk Southern worked closely with Amberg Technologies to provide a clear overview of the current measuring methods, requirements, challenges and risks associated with HWLs. Amberg then developed a prototype system (with patent pending) and successful tests have been completed at both a point of origin for NS shipments and at a location where HWLs are received at interchange. The measuring system consists of a tripod mounted laser, a specially designed track reference target (TRT) and software designed specifically for HWL measurements. The system allows car inspectors to take measurements from a safe, strategic location away from the car. As a result, this system eliminates the need to climb on the equipment or a ladder and greatly reduces the amount of time spent on and around live tracks. In addition, initial tests indicate that this technology reduces the labor time required to measure HWLs by as much as one half while improving measurement accuracy. These tests have demonstrated that a laser-based system has the potential to greatly improve the safety, efficiency and accuracy associated with measuring HWLs.

The Enhanced CANDU 6® (EC6®) is a nuclear reactor based on the proven CANDU 6 reactor that uses natural uranium and heavy water as a coolant and as a moderator. The EC6 has many enhancements to improve production and to meet next generation reactor standards. A modern distributed control system is used coupled with a new plant display system to facilitate the operation of the EC6. Computerization of safety systems is extended to include neutronic trip parameters and the Emergency Core Cooling (ECC) system. Computerized systems allow for thorough verification during their development, minimizing the installation, commissioning and maintenance times. Testing of these safety systems is also computer assisted to reduce the time required for testing and reduce the possibility of spurious trips. Enhanced safety system monitoring and equipment monitoring systems are used for early detection of faults or deterioration of equipment. This can in turn allow more targeted maintenance activities and improved safety. Critical safety parameter monitoring is also included in the design. Fission chambers are used instead of ion chambers for measuring neutron power. Fission chambers can extend the bottom of the power measurement range by three decades, allowing outages and restart of the reactor to be performed without the installation of startup instrumentation. Instrumentation is included to cater for Beyond Design Basis Accidents (BDBA). Additional battery banks are also included in the design to cater for Station Black-Out (SBO) and extended SBO. Connection provisions for mobile power generators have also been included in the design. The safety systems are controlled by modern equipment qualified to international standards for safety systems. The instrumentation required for these systems is also assessed for the severe conditions to which they might be exposed. Lessons learned from Fukushima on protection from external events are included in the design.

Reactor protection system is one of the most important safety systems in nuclear power plant and shall be designed with very high reliability. Digital computer-based Reactor Protection System (RPS) takes great advantages over its conventional counterpart based on analog technique and faces the issues how to effectively demonstrate and confirm the completeness and correctness of the software that performs reactor safety functions in the same time. It is commonly accepted that the essential way to solve safety software issues in a digital RPS is to pass a strict and independent Verification and Validation (V&V) process, in which integrated RPS testing play an important role to form a part of the overall system validation. Integrated RPS testing must be carried out rigorously before the system is delivered to nuclear power plant. The integrated testing are often combined with the factory acceptance test (FAT) to form a single testing activity, during which the RPS is excited by emulated static and dynamic input signals. The integration testing should simulate normal operation, anticipated operational occurrences and accident conditions, as well as anticipated faults on the inputs to the DRPS such as sensors out of range or ambiguous input readings. All safety function requirements of digital RPS should be confirmed by representative testing. The design and development of a test facility to carry out the integrated RPS testing are covered in this paper, which is merged in the research on a digital RPS engineering prototype for a nuclear power plant. The test facility is based on PXI platform and LabVIEW software development environment and its architecture design also takes into account the test functions future extensions such as hardware upgrades and software modules enhancement. The test facility provides the digital RPS with redundant, synchronized and multi-channel emulated signals that are produced to emulate all protection signals from 1E class sensors and transmitters with time varied value within their possible ranges, which would put integrated RPS testing into practice to confirm the digital RPS has fully met its predefined safety functionality requirements. The designed test facility can provide an independent verification and validation process for the research of digital RPS with scientific methods and authentic data to evaluate the RPS performance thoroughly and effectively, such as measuring threshold precision and trip response time, analyzing system statistical reliability and so on.

Safety demonstration tests on the 10 MW High Temperature Gas-cooled Reactor-Test Module (HTR-10) were conducted to verify the inherent safety characteristics of modular High Temperature Gas-cooled Reactors (HTGRs) as well as to obtain the reactor core and primary cooling system transient data for validation of HTGR safety analysis models and codes. As one of these safety demonstration tests, a simulated anticipated transient without scram (ATWS) test called loss of forced cooling by tripping the helium circulator without reactor scram was carried out at 100% rated power level in July, 2005. This paper simulates the reactor transient behaviour during the test by using the THERMIX code system. The reactor power transition and a comparison with the test result are presented. Owing to the negative temperature coefficient of reactivity, the reactor undergoes a self-shutdown after the stop of the helium circulator and keeps subcritical till the end of the test. Due to the loss of forced cooling, the residual heat is slowly transferred from the core to the Reactor Cavity Cooling System (RCCS) by conduction, radiation and natural convection. The thermal response of this heat removal process is investigated. The calculated and test temperature transients of the measuring points in the reactor internals are given and the differences are preliminarily discussed. With respect to the safety features of the HTR-10, it is of most importance that the maximum fuel center temperature is always lower than 1230 °C which is the limited value at the first phase of the HTR-10 project. The simulation and test results show that the HTR-10 has the built-in passive safety features, and the THERMIX code system is applicable and reasonable for simulating and analyzing the helium circulator trip ATWS test.

The market requirements with regard to transient operation capabilities of gas turbines (GT) in utility use are becoming stringent. Besides normal frequency support features, gas turbines in local electrical grids are often required to maintain the grid frequency under various situations, including emergencies, such as, loss of national grid connection or trip of a large consumer, etc. These requirements demand high performance and stability of GT control. On the other hand, the environmental aspects are becoming increasingly a public concern. In the past decades, remarkable progress has been made in combustion technologies of heavy-duty gas turbines. Lean premixing is a preferred technology for NOx emission reduction. Because of its flashback and extinction limits, a premix flame has usually a much narrower operation range compared to a diffusional one, adding tight constraint on GT control. This paper demonstrates a successful implementation of a model-based predictor, a proven control technique, in the closed loop control of the ALSTOM GT11N2 gas turbine. First, an online GT model, which is integrated into the GT control algorithm, was developed. By applying appropriate assumption and simplification, this model is capable of simulating the GT process over the whole load operation range with high dynamic accuracy. Secondly, a model-based predictor for accelerating slow measured signals was implemented. It dynamically compensates the system delays in the GT process and in the measuring instruments. Thirdly, the predictor was applied to the GT core control by replacing the measured signals with the accelerated signals. The original control structure was kept unchanged. In order to verify its performance and stability, the new control technique was validated on a real engine. Successful engine tests proved that the model-based predictor improves GT transient operation capabilities.