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Journal articles on the topic 'Mediated Culture'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 January 2023

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1

Niazian, M., S. A. Sadat Noori, P. Galuszka, and S. M. M. Mortazavian. "Tissue culture-based Agrobacterium-mediated and in planta transformation methods." Czech Journal of Genetics and Plant Breeding 53, No. 4 (2017): 133–43. http://dx.doi.org/10.17221/177/2016-cjgpb.

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Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods

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2

Corner, John. "Mediated persona and political culture." European Journal of Cultural Studies 3, no. 3 (2000): 386–402. http://dx.doi.org/10.1177/136754940000300306.

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3

Ghislandi, Patrizia, Juliana Raffaghelli, and Nan Yang. "Mediated Quality." International Journal of Digital Literacy and Digital Competence 4, no. 1 (2013): 56–73. http://dx.doi.org/10.4018/jdldc.2013010106.

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Even if the question of eLearning quality has been intensely discussed in the recent years, with several approaches and models arising, consistent transferring of concepts into practices is still difficult (Elhers & Hilera, 2012). In fact, eLearning is given different importance by the several stakeholders; consequently, the educational institutions’ culture of quality –meanings, discourses, representations and practices- is highly variable (Ehlers & Schneckenberg, 2010) and adapting to external frameworks and models of quality could be difficult. As a result, the implementation of qua

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4

Jacinto, Stella Santana da Silva, and Danieli Aparecida Duarte. "Jogo digital, cultura real: cultura indígena mediada pelas TDIC / Digital game, real culture: indigenous culture mediated by ICTs." Brazilian Journal of Development 8, no. 3 (2022): 17453–68. http://dx.doi.org/10.34117/bjdv8n3-129.

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5

Ural, Evran E., Victoria Toomajian, Ehsanul Hoque Apu, et al. "Visualizing Extracellular Vesicles and Their Function in 3D Tumor Microenvironment Models." International Journal of Molecular Sciences 22, no. 9 (2021): 4784. http://dx.doi.org/10.3390/ijms22094784.

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Extracellular vesicles (EVs) are cell-derived nanostructures that mediate intercellular communication by delivering complex signals in normal tissues and cancer. The cellular coordination required for tumor development and maintenance is mediated, in part, through EV transport of molecular cargo to resident and distant cells. Most studies on EV-mediated signaling have been performed in two-dimensional (2D) monolayer cell cultures, largely because of their simplicity and high-throughput screening capacity. Three-dimensional (3D) cell cultures can be used to study cell-to-cell and cell-to-matrix

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6

Shih, Chung-ling. "Robot-mediated Culture Education: Users’ Reception." International Journal of Technology in Education 5, no. 2 (2022): 280–95. http://dx.doi.org/10.46328/ijte.221.

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Culture education can use a myriad of media, and this research explores how a robot can be used for culture education. To conduct the research, a questionnaire-based survey was conducted on adult and non-adult users. The findings showed that the two groups of respondents when calculated together had a higher level of satisfaction with educational effectiveness and the significant value of the system (89%, 917/1030) than operational experience (84%, 862/1030) and the robot’s sensory appeal (77%, 797/1030). The findings suggested that respondents had attached greater importance to the significan

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7

Lalitha, K., K. R. Swaminathan, C. M. Vargheese, V. P. Shanthi, and R. Padma Bai. "Methanogenesis mediated by methylotrophic mixed culture." Applied Biochemistry and Biotechnology 49, no. 2 (1994): 113–34. http://dx.doi.org/10.1007/bf02788546.

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8

Aliotta, Jason M., Mandy Pereira, Mark Dooner, et al. "Microvesicle Mediated Genetic Phenotype Modulation." Blood 114, no. 22 (2009): 4509. http://dx.doi.org/10.1182/blood.v114.22.4509.4509.

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Abstract Abstract 4509 Objective We have previously reported that lung-derived microvesicles (MVs) can enter target marrow cells, resulting in increased levels of lung-specific mRNAs (Stem Cells 25:2245, 2007). Marrow cells which have been exposed to MVs also show increased production of pulmonary epithelial cells after transplantation into irradiated mice. The present studies have addressed the universality of the mRNA modulation and the underlying mechanisms. Methods/Results Co-culture of heart, brain, liver, and lung tissue across from murine marrow, but separated by a 0.4 micron cell-imper

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9

Abdelhakim, Haitham, Luis M. Cortez, Meizhang Li, et al. "LAG3 Inhibition Decreases AML-Induced Immunosuppression and Improves T Cell-Mediated Killing." Blood 134, Supplement_1 (2019): 3605. http://dx.doi.org/10.1182/blood-2019-129455.

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Background: Acute Myeloid Leukemia (AML) is an aggressive hematologic malignancy with known immune dyregulation. In addition to their capacity to rapidly divide, AML cells directly inhibit the activation and proliferation of immune cells in culture. Immunosuppressive features observed in the bone marrow of AML patients include upregulation of Tregs and production of immunosuppressive cytokines (e.g., TGFβ). Irradiating AML cells diminishes their immunosuppressive capacity while maintaining antigen presentation, leading to increased activation of T cells in co-culture. We subsequently identifie

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10

Quesenberry, Peter J. "Cell Fate Modulation by Microvesicles: Transcriptionally-Mediated and Long Term in Nature." Blood 118, no. 21 (2011): 4801. http://dx.doi.org/10.1182/blood.v118.21.4801.4801.

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Abstract Abstract 4801 Cell-derived membrane enclosed vesicles containing mRNA, protein, microRNA, and DNA, can enter cells and effect a phenotype change. We have shown that lung-derived microvesicles enter marrow cells inducing them to express pulmonary epithelial cell-specific protein and mRNA, a variety of microRNA and to enhance their capacity to engraft in irradiated mice and express the phenotype of type II pneumocytes (Aliotta et al, Exp Hematol 38,2010). In the present studies using rat/mouse hybrid cultures and measuring species-specific mRNA, we have shown that immediately after co-c

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11

Araki, Daisuke, Stefan Cordes, Fayaz Seifuddin, et al. "Notch-Mediated Expansion of Human Hematopoietic Stem and Progenitor Cells By Culture Under Hypoxia." Blood 136, Supplement 1 (2020): 28–29. http://dx.doi.org/10.1182/blood-2020-141121.

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Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). Recent studies have highlighted how increased proliferative demand in culture can trigger endoplasmic reticulum (ER) stress and impair HSC function. Here, we investigated whether ex vivo culture of HSPCs under hypoxia

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12

Read, Oliver James, Jennifer Bré, and David J. Harrison. "Abstract 1113: NUC-3373 potentiates immune-mediated cytotoxicity of CRC cells." Cancer Research 82, no. 12_Supplement (2022): 1113. http://dx.doi.org/10.1158/1538-7445.am2022-1113.

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Abstract Background: NUC-3373 is a phosphoramidate modification of fluorodeoxyuridine-monophosphate (FUDR-MP), the active anti-cancer metabolite of fluorouracil (5-FU), which binds and inhibits thymidylate synthase (TS), disrupting DNA synthesis and repair. NUC-3373 is designed to bypass 5-FU resistance mechanisms associated with transport, activation and breakdown, reduce generation of toxic metabolites, and deliver higher levels of active metabolite FUDR-MP to tumors. Our previous work demonstrated that NUC-3373 causes colorectal cancer (CRC) cell lines to release damage associated molecular

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13

Jerslev, Anne. "The Mediated Body." Nordicom Review 27, no. 2 (2006): 133–51. http://dx.doi.org/10.1515/nor-2017-0235.

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Abstract Taking Vivian Sobchack’s idea of the digital morph as not only a digital practice but also a metaphor for a culture obsessed with the idea of bodily changes, of reversibility and metamorphoses, the article takes a closer look at the visual construction of the body as a site of transformation, modification, and improvement in both television, film and fashion photography. The article focuses on the two reality programmes /Extreme Makeover/ (ABC) and /The Swan/ (FOX), the American drama-series /Nip/Tuck/ and an extended series of fashion photographs from /Italian Vogue/ July 2005 by Ame

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14

Minson, Katherine A., Madeline G. Huey, Amanda A. Hill, et al. "Bone Marrow Stromal Cell Mediated Resistance to Mertk Inhibition in Acute Leukemia." Blood 128, no. 22 (2016): 2819. http://dx.doi.org/10.1182/blood.v128.22.2819.2819.

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Abstract MERTK is a receptor tyrosine kinase of the TAM family (TYRO-3, AXL, MERTK) that is ectopically expressed in 30-50% of newly diagnosed pediatric acute lymphoblastic leukemia (ALL) patient samples and aberrantly expressed in 80-100% of pediatric and adult primary acute myeloid leukemia (AML) samples. MERTK inhibition mediated by shRNA or a small molecule inhibitor, MRX-2843, decreased colony-forming potential and induced apoptosis in leukemia cell cultures. Moreover, MERTK inhibition prolonged survival in mouse xenograft models of acute leukemia, but was not curative. In these models, t

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15

Rashedi, Iran, Alejandro Gomez-Aristizábal, Xinghua Wang, Sowmya Viswanathan, and Armand Keating. "TLR3 or TLR4 Activation Enhances MSC-Mediated Treg Generation Via Notch Signaling." Blood 126, no. 23 (2015): 3604. http://dx.doi.org/10.1182/blood.v126.23.3604.3604.

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Abstract Mesenchymal stromal cells (MSCs) are used as cell therapy for a variety of disorders, largely because of their immunosuppressive and regenerative functions by exerting immune effects via direct and indirect interactions with many types of immune cells. MSCs recruit and promote the generation of regulatory T cells (Tregs) both in vitro and in vivo. Toll-like receptors (TLRs), known for roles in innate and adaptive immunity, are involved in numerous pathological conditions, including graft-versus-host disease (GVHD). Several TLRs, especially TLR3 and TLR4, are highly expressed on MSCs a

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16

Little, Ben, and Alison Winch. "“just hanging out with you in my back yard”: Mark Zuckerberg and Mediated Paternalism." Open Cultural Studies 1, no. 1 (2018): 417–27. http://dx.doi.org/10.1515/culture-2017-0039.

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Abstract In a video that showcases a new Facebook feature, Mark Zuckerberg chats to his users, telling them that he’s “just hanging out with you in my backyard.” In this video-which is on his Facebook page-Zuckerberg discloses the domestic space of his backyard, revealing his interaction with family and friends. Depicted hosting a barbeque while watching the electoral debate, Zuckerberg performs an affective white postfeminist paternity (Hamad, 2014) by talking about hunting, eating meat, and being a father. This video is key in explaining how Zuckerberg affectively models patriarchal power. W

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17

Gibson, Margaret. "Death and mourning in technologically mediated culture." Health Sociology Review 16, no. 5 (2007): 415–24. http://dx.doi.org/10.5172/hesr.2007.16.5.415.

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18

Brockman, Kiersten. "Mediated intimacy: sex advice in media culture." Communication Review 24, no. 1 (2021): 83–85. http://dx.doi.org/10.1080/10714421.2021.1908780.

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19

Deller, Ruth A. "Mediated intimacy: sex advice in media culture." Journal of Gender Studies 28, no. 6 (2019): 746–47. http://dx.doi.org/10.1080/14753634.2019.1630079.

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20

Lumby, Catharine. "Review: Mediated Women: Representations in Popular Culture." Media International Australia 95, no. 1 (2000): 275. http://dx.doi.org/10.1177/1329878x0009500134.

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21

Barker, Meg-John, Rosalind Gill, and Laura Harvey. "Mediated intimacy: Sex advice in media culture." Sexualities 21, no. 8 (2018): 1337–45. http://dx.doi.org/10.1177/1363460718781342.

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22

Lobner, D., and M. Asrari. "Neurotoxicity of Dental Amalgam is Mediated by Zinc." Journal of Dental Research 82, no. 3 (2003): 243–46. http://dx.doi.org/10.1177/154405910308200318.

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The use of dental amalgam is controversial largely because it contains mercury. We tested whether amalgam caused toxicity in neuronal cultures and whether that toxicity was caused by mercury. In this study, we used cortical cell cultures to show for the first time that amalgam causes nerve cell toxicity in culture. However, the toxicity was not blocked by the mercury chelator, 2,3-dimercaptopropane-1-sulphonate (DMPS), but was blocked by the metal chelator, calcium disodium ethylenediaminetetraacetate (CaEDTA). DMPS was an effective mercury chelator in this system, since it blocked mercury tox

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23

Kurtar, E. S., A. Balkaya, and M. Ozbakir Ozer. "Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)." Czech Journal of Genetics and Plant Breeding 54, No. 1 (2018): 9–16. http://dx.doi.org/10.17221/30/2017-cjgpb.

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Although haploids were successfully produced via irradiated pollen technique and anther culture in Cucurbita maxima and Cucurbita moschata, the haploidization efficiency is still low due to genotype dependence. Thus, as an alternative technique, the efficacy of the ovule culture was investigated. Ovules were extracted at different flowering time and then cultured on a solid MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP), thidiazuron (TDZ), and naphthaleneacetic acid (NAA) to induce callogenesis and plant regeneration. The gynogenic response was infl

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24

Wallace, Charles S., та George A. Truskey. "Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-α-mediated endothelial cell activation". American Journal of Physiology-Heart and Circulatory Physiology 299, № 2 (2010): H338—H346. http://dx.doi.org/10.1152/ajpheart.01029.2009.

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We used a direct-contact endothelial cell-smooth muscle cell (EC-SMC) co-culture to examine whether quiescent SMCs regulate the EC inflammatory response to tumor necrosis factor (TNF)-α. ECs were cultured under static and physiological flow conditions. Compared with TNF-α-treated ECs in monoculture, TNF-α-treated ECs in co-culture had less NF-κB nuclear translocation; less intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin surface protein expression; no change in TNF receptor expression, but greater Kruppel-like factor 2 (KLF2) gene expressio

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25

Renzulli, J. F., G. Dooner, C. Owens, et al. "Microvesicular-mediated gene transfer of prostate tumor markers." Journal of Clinical Oncology 27, no. 15_suppl (2009): e16076-e16076. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.e16076.

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e16076 Background: Microvesicles have been a subject of research for many years. Recent work has focused on the potential for cancer vaccines via microvesicles. It has also been demonstrated that various cell-specific phenotypes can be transferred from one cell type to another through microvesicle transfer. Studies in our laboratory have demonstrated that co-culture of murine lung tissue with marrow cells across a cell impermeable membrane can induce elevations in lung-specific mRNA expression in human donor marrow stem cells. Our objective is to determine whether there is transfer of genetic

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26

Yazdanparast, Atefeh, Iman Naderi, Nancy Spears, and Robert O. Fabrize. "Advertising and Pseudo-Culture." Journal of Macromarketing 38, no. 2 (2018): 185–205. http://dx.doi.org/10.1177/0276146718762475.

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This study investigates the use of mass media, specifically advertising, in cultural transformation projects to weaken cultures and replace them with a crafted pseudo-culture. We rely on Adorno’s theory of pseudo-culture to examine how political ideologies shape cultural transformation using mass-mediated ad images. Following a content analysis and a semiotic analysis of print advertisements over a period of 48 years, we identify five major themes underlying pseudo-culture formation and the advertising strategies implemented to support these themes. This work also identifies four major tools u

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27

Rogove, A. D., C. Siao, B. Keyt, S. Strickland, and S. E. Tsirka. "Activation of microglia reveals a non-proteolytic cytokine function for tissue plasminogen activator in the central nervous system." Journal of Cell Science 112, no. 22 (1999): 4007–16. http://dx.doi.org/10.1242/jcs.112.22.4007.

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Tissue plasminogen activator mediates excitotoxin-induced neurodegeneration and microglial activation in the mouse hippocampus. Here we show that tissue plasminogen activator (tPA) acts in a protease-independent manner to modulate the activation of microglia, the cells of the central nervous system with macrophage properties. Cultured microglia from tPA-deficient mice can phagocytose as efficiently as wild-type microglia. However, tPA-deficient microglia in mixed cortical cultures exhibit attenuated activation in response to lipopolysaccharide, as judged by morphological changes, increased exp

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Morin, Caroline, та Eric Rousseau. "Enhanced Ca2+ sensitivity in hyperresponsive cultured bronchi is mediated by TNFα and NF-κB". Canadian Journal of Physiology and Pharmacology 84, № 10 (2006): 1029–41. http://dx.doi.org/10.1139/y06-048.

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The mechanical properties and Ca2+ sensitivity of an organ-culture model derived from guinea pig airways have been examined. The cultured explants develop airway hyperresponsiveness to pharmacological agonists after 3-day culture, when compared with fresh and ovalbumin-sensitized tissues. The reactivity of cultured explants is dependent on the presence of the epithelium. They are also sensitive to glucocorticosteroid pretreatments, which neutralize the TNFα antibody and ΝF-κB inhibitor. Hence, specific immunostaining of ΝF-κB subunits (p65 and p50) was increased in the nuclear extract of cultu

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Payne, Robert. "Lossy Media: Queer Encounters with Infrastructure." Open Cultural Studies 2, no. 1 (2018): 528–39. http://dx.doi.org/10.1515/culture-2018-0048.

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Abstract In an era of “frictionless” digital environments, this article proposes a queer analysis of the “lossy” materialities of mediated encounters. Building on recent scholarship on media failure and media infrastructures, it will argue that moments of disruption and deterioration commonly experienced by users reveal the failure of overlapping social and technical infrastructures to ensure lossless transmission of normative fantasies of subjectivity and mediated relationality. Highlighting the queer instability of material assemblages, it will pay close attention to how the articulation of

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30

Suzdaltseva, Yulia, Kirill Goryunov, Ekaterina Silina, Natalia Manturova, Victor Stupin, and Sergey L. Kiselev. "Equilibrium among Inflammatory Factors Determines Human MSC-Mediated Immunosuppressive Effect." Cells 11, no. 7 (2022): 1210. http://dx.doi.org/10.3390/cells11071210.

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Mesenchymal stem cells (MSCs) are thought to be a promising therapeutic agent due to their multiple paracrine and immunomodulatory properties, providing protection from chronic inflammation and promoting tissue repair. MSCs can regulate the balance of pro-inflammatory and anti-inflammatory factors in inflamed tissues, creating a microenvironment necessary for successful healing; however, their interactions with immune cells are still poorly studied. We examined the temporal and spatial changes in gene regulation and the paracrine milieu accompanying the MSC-mediated immunosuppression effect in

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31

Kumar, Palani Kumar, Saravanan Ganesan, Nithya Balasundaram, et al. "Targeting Extracellular Vesicle Secretion As a Strategy to Overcome Microenvironment Mediated Drug Resistance in Acute Myeloid Leukemia." Blood 134, Supplement_1 (2019): 3733. http://dx.doi.org/10.1182/blood-2019-126389.

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Increasing evidence suggests that bone marrow microenvironment act as a sanctuary site for acute myeloid leukemia (AML) cells and provides protection from conventional chemotherapy agents. Recently, extracellular vesicles (EVs) have attracted substantial attention as a carrier of complex intercellular information by transferring microRNA, mRNA and proteins. We undertook a study to delineate the molecular mediators and potential role of extracellular vesicles in stromal microenvironment mediated drug resistance in AML. We performed a series of in vitro experiments with AML cell lines (U937, THP

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32

Tsang, Kwok Kuen, Yuan Teng, Yi Lian, and Li Wang. "School Management Culture, Emotional Labor, and Teacher Burnout in Mainland China." Sustainability 13, no. 16 (2021): 9141. http://dx.doi.org/10.3390/su13169141.

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The literature suggests that teacher burnout is influenced by the market and hierarchy cultures of school management and teachers’ emotional labor strategies of surface and deep acting. However, studies have suggested that school management cultures and emotional labor strategies may not function independently based on the emotional labor theory. Nevertheless, the literature has paid less attention to the relationship between the school management cultures, emotional labor, and teacher burnout. Therefore, this study aims to investigate the relationship between the three variables in China via

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33

PÉREZ-PERTEJO, YOLANDA, RAQUEL ÁLVAREZ-VELILLA, CARLOS GARCÍA ESTRADA, RAFAEL BALAÑA-FOUCE, and ROSA M. REGUERA. "Leishmania donovani: proteasome-mediated down-regulation of methionine adenosyltransferase." Parasitology 138, no. 9 (2011): 1082–92. http://dx.doi.org/10.1017/s0031182011000862.

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SUMMARYMethionine adenosyltransferase (MAT) is an important enzyme for metabolic processes, to the extent that its product, S-adenosylmethionine (AdoMet), plays a key role intrans-methylation,trans-sulphuration and polyamine synthesis. Previous studies have shown that a MAT-overexpressing strain ofLeishmania donovanicontrols AdoMet production, keeping the intracellular AdoMet concentration at levels that are compatible with cell survival. This unexpected result, together with the fact that MAT activity and abundance changed with time in culture, suggests that different regulatory mechanisms ac

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34

Cho, Sun Wook, Young A. Kim, Hyun Jin Sun, et al. "CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma." Endocrine-Related Cancer 23, no. 2 (2015): 113–24. http://dx.doi.org/10.1530/erc-15-0196.

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Macrophages in tumor microenvironment have pivotal roles in tumor growth, metastasis, and angiogenesis. We investigated the interacting mechanism of macrophage actions in human papillary thyroid cancer (PTC). Co-cultures of macrophage/PTC significantly increased the cancer cell migration potentials, compared with the PTC culture alone. Treatment of conditioned medium (CM) of macrophage/PTC co-cultures enhanced cell invasions in 3D invasion assay. Cytokine array analysis demonstrated that CM of macrophage/PTC co-cultures contained a high level of CXCL16, while it was not found in CM of PTC cult

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Nikolaienko, T. V., V. V. Nikulina, D. V. Shelest, and L. V. Garmanchuk. "The mechanism of VEGF-mediated endothelial cells survival and proliferation in conditions of unfed-culture." Ukrainian Biochemical Journal 88, no. 4 (2016): 12–19. http://dx.doi.org/10.15407/ubj88.04.012.

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36

Marumoto, Y., I. Sato, and K. Ikeda. "Effects of Culture Supernatants of Leukocytes from Periodontal Patients on Monocyte-Mediated Hydroxyapatite Resorption." Advances in Dental Research 2, no. 2 (1988): 368–71. http://dx.doi.org/10.1177/08959374880020022901.

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In this study, the effects of culture supernatants on various activities of the monocyte, as a bone-resorbing cell, were compared between peripheral blood leukocyte (PBL) cultures from patients with periodontal disease and those from subjects with a clinically healthy periodontium. We have reported that normal human monocytes in vitro induce the release of calcium from synthetic hydroxyapatite particles and that the activity is enhanced by supernatants from cultures of stimulated or non-stimulated peripheral blood leukocytes. Monocytes from both patients and healthy subjects induced the releas

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Alberts, Philipp, Rachel Rudge, Ina Hinners, et al. "Cross Talk between Tetanus Neurotoxin-insensitive Vesicle-associated Membrane Protein-mediated Transport and L1-mediated Adhesion." Molecular Biology of the Cell 14, no. 10 (2003): 4207–20. http://dx.doi.org/10.1091/mbc.e03-03-0147.

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The membrane-trafficking pathway mediated by tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) in neurons is still unknown. We show herein that TI-VAMP expression is necessary for neurite outgrowth in PC12 cells and hippocampal neurons in culture. TI-VAMP interacts with plasma membrane and endosomal target soluble N-ethylmaleimide-sensitive factor attachment protein receptors, suggesting that TI-VAMP mediates a recycling pathway. L1, a cell-cell adhesion molecule involved in axonal outgrowth, colocalized with TI-VAMP in the developing brain, neurons in culture, and P

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Alexander, J. Steven, and Thomas M. Crisp. "Gonadotrophic regulation of prolactin mediated progesterone secretion in vitro." Acta Endocrinologica 110, no. 2 (1985): 251–56. http://dx.doi.org/10.1530/acta.0.1100251.

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Abstract. The effects of preincubating rat granulosa cells with FSH, LH, and Prl on subsequent Prl mediated progesterone secretion were investigated. Granulosa cells were isolated from ovarian follicles 50 h after injection of 5 IU PMSG and were then plated on poly-l-lysine coated coverslips in serum supplemented medium. Cells were preincubated for 24 h in the absence of hormones (control) or with the addition of either 0.25, 2.5, 25 ng/ml rat FSH or rat LH, or 1 μg/ml rat Prl. Following the preincubation period, cells were maintained for an additional 6 or 8 days in the presence or absence of

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Zuckermann, F. A., and J. R. Head. "Expression of MHC antigens on murine trophoblast and their modulation by interferon." Journal of Immunology 137, no. 3 (1986): 846–53. http://dx.doi.org/10.4049/jimmunol.137.3.846.

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Abstract Primary cultures of defined populations of mouse trophoblast, isolated from mature placentas, were analyzed for their MHC antigen expression and for the modulatory effect of interferon (IFN) by antibody- and complement-mediated cytotoxicity and flow cytofluorometry. The cells were obtained from placentas by enzymatic digestion, followed by Percoll gradient fractionation, and are large, fetally derived epithelial cells, which we previously characterized and identified as trophoblast cells. After 2 days in culture, a significant proportion of the trophoblast cells were susceptible to an

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DAVEREY, AMITA, AUSTIN C. MYTTY, and SRIVATSAN KIDAMBI. "TOPOGRAPHY MEDIATED REGULATION OF HER-2 EXPRESSION IN BREAST CANCER CELLS." Nano LIFE 02, no. 03 (2012): 1241009. http://dx.doi.org/10.1142/s1793984412410097.

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This article demonstrates that the surface micro-topography regulates the biology of breast cancer cells, including the expression of HER-2 gene and protein. The breast tumor microenvironment is made up of heterogenous mixture of pores, ridges and collagen fibers with well defined topographical features. Although, significant progress has been achieved towards elucidating the biochemical and molecular mechanisms that underlie breast cancer progression, quantitative characterization of the associated mechanical/topographical properties and their role in breast tumor progression remains largely

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Pilarski, L. M., H. Miszta, and E. A. Turley. "Regulated expression of a receptor for hyaluronan-mediated motility on human thymocytes and T cells." Journal of Immunology 150, no. 10 (1993): 4292–302. http://dx.doi.org/10.4049/jimmunol.150.10.4292.

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Abstract A receptor for hyaluronan-mediated motility (RHAMM) has been shown to promote cell locomotion. Among human T lineage lymphocytes, RHAMM is expressed only on a subset of thymocytes, being absent on mature peripheral T cells from blood, spleen, and lymph node. Among thymocytes, RHAMM is selectively expressed on a subset of CD3+ CD45RA+R0+ cells, and functions in motility as shown by the ability of anti-RHAMM to reduce the speed of thymocyte locomotion from 11 microns/minute to 3 microns/min. Although freshly isolated multi-negative (MN) thymocytes (CD3-4-8-19-) lack RHAMM, its expressio

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Mingus, Matthew S. "Toward Understanding the Culture of Internet-Mediated Learning." Journal of Public Affairs Education 5, no. 3 (1999): 225–35. http://dx.doi.org/10.1080/15236803.1999.12022073.

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Koh, JY, MP Goldberg, DM Hartley, and DW Choi. "Non-NMDA receptor-mediated neurotoxicity in cortical culture." Journal of Neuroscience 10, no. 2 (1990): 693–705. http://dx.doi.org/10.1523/jneurosci.10-02-00693.1990.

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Ess, Charles, and Fay Sudweeks. "Culture and Computer-Mediated Communication: Toward New Understandings." Journal of Computer-Mediated Communication 11, no. 1 (2005): 179–91. http://dx.doi.org/10.1111/j.1083-6101.2006.tb00309.x.

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Orgeret, Kristin Skare. "Mediated Culture and the Well-informed Global Citizen." Nordicom Review 31, no. 2 (2010): 47–61. http://dx.doi.org/10.1515/nor-2017-0129.

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Abstract During recent years, considerable attention has been paid to the negative portrayal of the African continent in the media of the so-called ‘global North’. Significantly less focus has been put on how to actually represent Africa in the news as more than the site of catastrophes or in other ways than through sunshine stories of the ‘struggling but smiling African’. The present article argues that the lack of a wide range of different genres in the North’s mediated representations of Africa is problematic, because the ‘hard news’ we receive is deficient in information about the backgrou

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Metz, J. Michel. "Computer‐mediated communication and the question of culture." New Jersey Journal of Communication 3, no. 1 (1995): 1–25. http://dx.doi.org/10.1080/15456879509367274.

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Nilsson, David, Angelica Wackenfors, Lotta Gustafsson, et al. "Endothelin receptor-mediated vasodilatation: Effects of organ culture." European Journal of Pharmacology 579, no. 1-3 (2008): 233–40. http://dx.doi.org/10.1016/j.ejphar.2007.09.031.

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Baldwin, Miriam. "The zoom-in culture of digitally-mediated identity." Journal for Cultural Research 24, no. 1 (2020): 53–68. http://dx.doi.org/10.1080/14797585.2020.1755506.

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Maynard, T. M., Y. Wakamatsu, and J. A. Weston. "Cell interactions within nascent neural crest cell populations transiently promote death of neurogenic precursors." Development 127, no. 21 (2000): 4561–72. http://dx.doi.org/10.1242/dev.127.21.4561.

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We have previously shown that cultured trunk neural crest cell populations irreversibly lose neurogenic ability when dispersal is prevented or delayed, while the ability to produce other crest derivatives is retained (Vogel, K. S. and Weston, J. A. (1988) Neuron 1, 569–577). Here, we show that when crest cells are prevented from dispersing, cell death is increased and neurogenesis is decreased in the population, as a result of high cell density. Control experiments to characterize the effects of high cell density on environmental conditions in culture suggest that reduced neurogenesis is the r

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Mallory, Heather S., Nicholas J. Gibson, Jon H. Hayashi, Alan J. Nighorn, and Lynne A. Oland. "Direct and glia-mediated effects of GABA on development of central olfactory neurons." Neuron Glia Biology 7, no. 2-4 (2011): 143–61. http://dx.doi.org/10.1017/s1740925x12000075.

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Previously studied for its role in processing olfactory information in the antennal lobe, GABA also may shape development of the olfactory pathway, acting either through or on glial cells. Early in development, the dendrites of GABAergic neurons extend to the glial border that surrounds the nascent olfactory lobe neuropil. These neuropil glia express both GABAA and GABAB receptors, about half of the glia in acute cultures responded to GABA with small outward currents, and about a third responded with small transient increases in intracellular calcium. The neuronal classes that express GABA in

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