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Journal articles on the topic 'Medicinal plant combination – Chromatographic analysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Kurkin, V. A., N. R. Varina, E. V. Avdeeva, L. D. Klimova, S. V. Pervushkin, and T. K. Ryazanova. "THE DEVELOPMENT OF THE COMBINED MEDICINAL HERBAL REMEDIES FOR STOMATOLOGICAL AND OTORHINOLARYNGOLOGICAL PRACTICE." Science and Innovations in Medicine 1, no. 4 (2016): 51–57. http://dx.doi.org/10.35693/2500-1388-2016-0-4-51-57.

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Aim - to develop a series of combined medicinal herbal remedies «Dentos» for dental and otorhinolaryngological practice, in which complex effect on different elements of the infectious inflammatory process is achieved by the combination of several groups of biologically active compounds. Materials and methods. Basic phytocomposition is a medicinal preparation "Dentos", which is composite alcohol-water extract on 70% ethanol from the five types of medicinal plant raw materials: the leaves of Eucalyptus viminalis, the herb of Echinacea purpurea, the flowers of Calendula officinalis, the bark of

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Nazarenko, D. V., I. A. Rodin, and O. A. Shpigun. "The use of machine learning in the analytical control of the preparations of medicinal plants." Industrial laboratory. Diagnostics of materials 84, no. 10 (2018): 67–78. http://dx.doi.org/10.26896/1028-6861-2018-84-10-67-78.

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Despite the fact that the global market for medicinal plants amounts to hundreds of billions of dollars, there is almost no government control over the quality of such pharmaceuticals in most countries of the world. This is partly attributed to the complex composition of plant materials: traditional analytical methodology is based on the use of standard reference samples for each analyte. In this case, preparations based on medicinal plants may contain tens and hundreds of physiologically active components. Isolation of those compounds in a pure form in practice is carried out using preparativ

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3

He, Ruoxu. "Quality Evaluation of Fritillaria thunbergii by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis." International Journal of Agriculture and Biology 25, no. 01 (2021): 131–38. http://dx.doi.org/10.17957/ijab/15.1647.

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Medicinal Zhebeimu refers to the dried bulb of the plant Fritillaria thunbergii, which belongs to the family Liliaceae. Their main production sites are in Zhejiang, Jiangsu, and Anhui Provinces, with alkaloids as the main components. In this study, according to Chinese Pharmacopoeia, different mobile phases were investigated. Because most alkaloids in Fritillaria do not have UV absorption, evaporative light scattering detection (ELSD) was used instead of UV to produce the HPLC-ELSD spectra for five F. thunbergii samples and other samples, e.g. F. chuanxiensis, F. anhuiensis, and F. ussuriensis

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Gupta, Dipankar Das, Md Enamul Haque, Md Nahidul Islam, Shafiqur Rahman, AKM Mahbub Hasan, and Baigid Alam Shibib. "Alkaloid and Steroid from the Stem Bark of Jatropha curcas (Euphorbiaceae)." Dhaka University Journal of Pharmaceutical Sciences 10, no. 1 (2012): 9–11. http://dx.doi.org/10.3329/dujps.v10i1.10009.

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The plant Jatropha curcas grows well in different parts of Bangladesh and used in many medicinal purposes locally. The alkaloid atherospermidine and a steroid stigmasterol were isolated from the ethyl acetate extract of the stem bark of J. curcas by a combination of column and preparative thin-layer chromatography over silica gel. The structures of these compounds were determined by spectroscopic analysis (UV, IR, 1H NMR and 13C NMR) and by comparison with published data. This is the first report of isolation of the alkaloid atherospermidine from this plant. DOI: http://dx.doi.org/10.3329/dujp

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5

VARNIKA, Varnika, Rohit SHARMA, Ajay SINGH, Shalini SHALINI, and Nishesh SHARMA. "Micropropagation and Screening of Phytocompounds Present among in vitro Raised and Wild Plants of Rauvolfia serpentine." Walailak Journal of Science and Technology (WJST) 17, no. 11 (2020): 1177–93. http://dx.doi.org/10.48048/wjst.2020.6492.

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Rauvolfia serpentina, commonly known as Sarpgandha, is a plant well-known for being utilized for several medicinal purposes. Unrestricted collection from natural stands and overexploitation for medicinal and traditional purposes have rendered it endangered, hence there is necessary requirement for the development of cultivation protocols for mass propagation and sustained utilization of the plant. In the present study, in vitro culture of the apical parts of R. serpentina on MS (Murashige and Skoog) medium enriched with NAA resulted in development of callus, whereas multiple shoot regeneration

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Yusuf, A. J., M. I. Abdullahi, A. M. Musa, A. K. Haruna, V. Mzozoyana, and A. Sanusi. "Isolation of Epicatechin from the Stem Bark of Neocarya macrophylla (Sabine) Prance (Chrysobalanaceae)." Nigerian Journal of Basic and Applied Sciences 27, no. 2 (2020): 101–7. http://dx.doi.org/10.4314/njbas.v27i2.14.

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Neocarya macrophylla has a wide range of medicinal uses in traditional medicine. The aim of the study was to isolate and characterize compound from the stem bark of the plant. (-)-Epicatechin (a flavan-3-ol) was isolated from the ethylacetate soluble fraction of the methanol stem bark extract of the plant using a combination of silica gel and sephadex LH-20 column chromatography. The structure of the compound as (-)-epicatechin was confirmed on the basis of chemical test, 1D- & 2D-NMR spectroscopy and comparison with existing data in literature. This is the first report of isolation of epi

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Nyongbela, Kennedy D., Felix L. Makolo, Thomas R. Hoye, and Simon MN Efange. "Isolation and Characterization of Sclerienone C from Scleria Striatinux." Natural Product Communications 11, no. 1 (2016): 1934578X1601100. http://dx.doi.org/10.1177/1934578x1601100102.

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Herein, we report the isolation and characterization of sclerienone C, a novel sesquiterpene isolated from the methylene chloride/methanol (1:1) extract of Scleria striatinux that we have deduced to have structure 1. This medicinal spice of Cameroon has been shown to display antimicrobial and antiplasmodial activities. The isolation and purification involved a combination of methods including silica gel column chromatography, Sephadex LH-20, and semi-prep HPLC separations. Structure elucidation was carried-out by means of spectroscopic analysis and comparison with previously isolated sesquiter

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8

Braccia, Clarissa, Bhakti Prinsi, Mara Colzani, et al. "Protocol Optimization of Proteomic Analysis of Korean Ginseng (Panax ginseng Meyer)." Separations 8, no. 4 (2021): 53. http://dx.doi.org/10.3390/separations8040053.

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The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing of the Panax ginseng has paved the way for in-depth proteomic studies of this medicinal plant. The current study was conducted to deepen the proteomic information on the root proteome of Korean ginseng. Proteomic workflow was optimized by testing two different strategies, characterized by the phenol extraction procedure, the presence or the absence of SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. The results

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9

Tian, Shuge, Qian Yu, Lude Xin, Zhaohui Sunny Zhou, and Halmuart· Upur. "Chemical Fingerprinting by RP-RRLC-DAD and Principal Component Analysis of Ziziphora clinopodioides from Different Locations." Natural Product Communications 7, no. 9 (2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700919.

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An efficient and accurate fingerprinting method using reversed-phase rapid-resolution liquid-chromatography coupled with photodiode array detection has been developed and optimized to examine the variance in active compounds among Ziziphora clinopodioides Lam from different locations. Three active components, diosmin, linarin and pulegone, were identified by matching their retention times and UV spectra with the corresponding reference compounds. Our results indicated that chromatographic fingerprints, in combination with principal component analysis (PCA) and hierarchical clustering analysis

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Ouattara, Zana A., Jean Brice Boti, Coffy Antoine Ahibo, Félix Tomi, Joseph Casanova, and Ange Bighelli. "Combined Analysis of the Root Bark Oil of Cleistopholis glauca by Chromatographic and Spectroscopic Techniques." Natural Product Communications 8, no. 12 (2013): 1934578X1300801. http://dx.doi.org/10.1177/1934578x1300801231.

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The composition of root bark oil from Cleistopholis glauca Pierre ex Engler & Diels growing wild in Ivory Coast was investigated by GC (in combination with retention indices) and 13C NMR spectroscopy after partition of hydrocarbons and oxygenated compounds on silica gel. Thirty-one compounds have been identified. C. glauca produces a sesquiterpene-rich oil, patchoulenone (33.5%), cyperene (9.5%) and germacrene D (6.6%) being the main components. Special attention was paid to the identification and quantification of germacrene C (a heat-sensitive compound) and δ-elemene, which were achieved

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11

Boussaïd, Maghnia, Chahrazed Bekhechi, Fewzia Atik-Bekkara, Mathieu Paoli, Joseph Casanova, and Félix Tomi. "Composition and Chemical Variability of the Cone Oil from Algerian Tetraclinis articulata." Natural Product Communications 11, no. 8 (2016): 1934578X1601100. http://dx.doi.org/10.1177/1934578x1601100834.

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Very little is known about the yield and chemical composition of the essential oil isolated from cones of Tetraclinis articulata (Vahl) Masters (Cupressaceae). In continuation of our on-going work devoted to the valorisation of medicinal and aromatic plants from Tlemcen Province (North-Western Algeria), cones have been harvested in eight locations (coastal township and highlands) and 40 oil samples have been isolated by hydrodistillation. Six oil samples were analyzed by a combination of chromatographic [GC(Retention indices)] and spectroscopic techniques (GC-MS, 13C NMR). Then, the remaining

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Kamboj, Anjoo, Ishtdeep Kaur, and Narinder Kaur. "Recent Advances in Standardization of Herbal Drugs." Current Traditional Medicine 6, no. 4 (2020): 278–99. http://dx.doi.org/10.2174/2215083805666190613141613.

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Background: Herbal drugs play a significant role to maintain the human healthiness and to treat the ailments since the dawn of civilization. Moreover, these plants have provided many lead compounds that culminated in modern medicine. A single herb is regarded as mini-combinatorial library of phytoconstituents hence the quality control of herbal drugs in an herbal formulation is not an easy task because a number of factors impact their pharmacological efficiency and consistent therapeutic effects. Hence, to provide consistent beneficial therapeutic effects, standardized herbal products of consi

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13

Al-Otibi, Fatimah, Reem A. Al-Ahaidib, Raedah I. Alharbi, Rana M. Al-Otaibi, and Gadah Albasher. "Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract." Molecules 26, no. 1 (2020): 130. http://dx.doi.org/10.3390/molecules26010130.

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The green biosynthesis of nanoparticles by plant extracts is an attractive and promising technique for medicinal applications. In the current study, we chose one of the daisy plants, Aaronsohnia factorovskyi (which grows in the Najd region, Saudi Arabia), to investigate its anti-microbial efficacy, in combination with silver nanoparticles. The biosynthesized nanoparticles were evaluated for antibacterial activity against Staphylococcus aureus, Bacillussubtilis (Gram-positive), Pseudomonas aeruginosa, and Escherichia coli, (Gram-negative) using the disc diffusion method, while the antifungal ac

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14

Lv, Hai-Ning, Xiao-Yu Guo, Peng-Fei Tu, and Yong Jiang. "Comparative Analysis of the Essential Oil Composition of Murraya Paniculata and M. exotica." Natural Product Communications 8, no. 10 (2013): 1934578X1300801. http://dx.doi.org/10.1177/1934578x1300801035.

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Murrayae Folium et Cacumen (MFC) is a traditional Chinese medicine (TCM) derived from the leaves and twigs of two aromatic species of Rutaceae: Murraya paniculata (L.) Jack and M. exotica L. It has long been used as a folk medicine in South China for the treatment of a variety of disorders, particularly for inflammatory lesions and pains. In the present study, a detailed chemical examination as well as a comparative analysis of the essential oil composition of M. paniculata and M exotica was conducted by gas chromatography-mass spectrometry (GC-MS). A total of 141 volatile components were iden

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15

Tashimbetova, A. B., A. K. Umbetova, Z. B. Halmenova, et al. "CHEMICAL STUDY OF LAVANDULA FERTILIZER." SERIES CHEMISTRY AND TECHNOLOGY 2, no. 440 (2020): 39–46. http://dx.doi.org/10.32014/2020.2518-1491.21.

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Genus Lavandula – specially grown as an aromatic and medicinal plant. In inflorescences, the content of essential oil is from 0.8 % to 2.6 %, in leaves up to 0.3 %. The main components of the essential oil are linalool (10-30 %) in the free state and its esters with acetic, butyric, valerianic, caproic acids (30-60 %), as well as geraniol, citral, borneolen, bisabolene, α-pinene and others. Currently, the plant is used as an ornamental, as a spice in cooking, as well as for medicinal purposes. In traditional medicine, flowers, leaves and branches of lavender are used. In Bulgaria, lavender is

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Katakam, Sagar, Rajeshwari Rathod, Poojadevi Sharma, et al. "HPLC Coupled with Chemometric Analysis and LC–MS Studies of Three Flavonoids in Tephrosia purpurea (L.) Pers Revealed Impact of Chemodiversity on the Quest for the Chemical Markers." Journal of Chromatographic Science 57, no. 9 (2019): 821–27. http://dx.doi.org/10.1093/chromsci/bmz058.

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Abstract Globally, Tephrosia purpurea (L.) Pers is used as an important component in herbal drug formulations for liver health. The present study is aimed to develop a suitable analytical approach for simultaneous analysis of three flavonoids (rutin, deguelin and rotenone) to establish quality control methods for plant. A novel High-performance liquid chromatography photodiode array detector (HPLC-PDA) method has been developed to quantify these flavonoids in T. purpurea. The method was validated, and data were subjected to chemometric analysis to select most optimal marker compound. The metho

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17

Domínguez, Fabiola, Marco Chávez, María Luisa Garduño-Ramírez, Víctor M. Chávez-Avila, Martín Mata, and Francisco Cruz-Sosa. "Honokiol and Magnolol Production by in vitro Micropropagated Plants of Magnolia dealbata, an Endangered Endemic Mexican Species." Natural Product Communications 5, no. 2 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500213.

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An efficient protocol for the in vitro propagation of Magnolia dealbata Zucc., an important medicinal plant that is the source of the anxiolytic and anticancer compounds honokiol and magnolol, was established. This plant is wild-crafted, and conservationists have expressed concerns with regard to the sustainability of production. In the present work, two factors were found to be of importance for the regeneration of M. dealbata and the production of honokiol and magnolol. These factors were the type of explants and the combination and concentration of plant-growth regulators. Green, compact, n

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18

Shibli, Rida A. "Manipulating Some Culturing Conditions Enhances Production of Solanine in Microshoots, Callus and Cell suspension Cultures of Solanum nigrum L.: A Wild Medicinal Plant." Journal of Agricultural and Marine Sciences [JAMS] 24 (February 10, 2020): 51. http://dx.doi.org/10.24200/jams.vol24iss0pp51-62.

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Solanum nigrum L. is a medicinal plant of solanaceae family with distinguished therapeutic properties. Traditionally, S. nigrum. had been used as an anti-tumorgenic, antioxidant, hepatoprotective, diuretic, and antipyretic agent. The most important alkaloid member in this plant is solanine. Therefore, this study was conducted to utilize tissue culture techniques for the enhancement of solanine production in the in vitro grown cultures of this promising neglected plant. For callus growth and development experimental part, the highest callus growth parameters (callus diameter (21.4 mm) and callu

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Sutour, Sylvain, François Luro, Joseph Casanova, and Félix Tomi. "Integrated Analysis by GC(RI), GC-MS and 13C NMR of Fortunella Japonica Leaf Volatiles Obtained by Hydrodistillation, Microwave- assisted Hydrodistillation and Hydrolate Extraction." Natural Product Communications 12, no. 3 (2017): 1934578X1701200. http://dx.doi.org/10.1177/1934578x1701200331.

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The chemical composition of the essential oil (EO), microwave extract (ME) and hydrolate extract (HE) from the same batch of leaves of Fortunella japonica, was investigated by combination of chromatographic (GC, CC) and spectroscopic techniques (GC-MS, 13C NMR). F. japonica essential oil and extracts are complex mixtures of 28–60 compounds being mainly oxygenated sesquiterpenes. The EO composition was dominated by germacrene D (14.9%), β-elemol (9.1%), cis-guai-6-en-10p-ol (6.3%), β-eudesmol (5.5%), and δ-elemene (5.2%). Limonene was the unique monoterpene identified at appreciable amount (7.1

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Albogami, Sarah, and Aziza Hassan. "Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models." Molecules 26, no. 13 (2021): 4069. http://dx.doi.org/10.3390/molecules26134069.

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Cancer is one of the most serious public health issues worldwide, ranking second only to cardiovascular diseases as a cause of death. Numerous plant extracts have extraordinary health benefits and have been used for centuries to treat a variety of ailments with few side effects. Olive leaves have a long history of medicinal and therapeutic use. In this study, the anti-cancer properties of an olive leaf extract were investigated in vitro using colorectal and prostate cancer cell lines (HT29 and PC3, respectively). A high-performance liquid chromatography analysis showed that the olive leaf extr

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Schobel, Uwe, Michel Frenay, Danny A. Van Elswijk, et al. "High Resolution Screening of Plant Natural Product Extracts for Estrogen Receptor a and f3 Binding Activity Using an Online HPLC-MS Biochemical Detection System." Journal of Biomolecular Screening 6, no. 5 (2001): 291–303. http://dx.doi.org/10.1177/108705710100600503.

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A new screening technology that combines biochemical analysis with the resolution power of high-performance liquid chromatography (HPLC), referred to here as high-resolution screening (HRS) technique, is described. The capability of the HRS technology to analyze biologically active compounds in complex mixtures is demonstrated by screening a plant natural product extract library for estrogen receptor (ER) a and fi binding activity. The simultaneous structure elucidation of biologically active components in crude extracts was achieved by operating the HRS system in combination with mass spectro

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Murugesan, Parameswari, and Kalaiselvi Senthil. "INFLUENCE OF AUXIN ON BIOMASS PRODUCTION AND WITHANOLIDE ACCUMULATION IN ADVENTITIOUS ROOT CULTURE OF INDIAN RENNET: WITHANIA COAGULANS." Asian Journal of Pharmaceutical and Clinical Research 10, no. 7 (2017): 131. http://dx.doi.org/10.22159/ajpcr.2017.v10i7.17342.

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Objective: Withanolides are the biologically active, principle compound present in Withania coagulans, which is having a high medicinal value and possesses potent therapeutic activity. The present study was attempted with an objective to investigate a biomass growth and withanolide production in in vitro root tissues of W. coagulans.Methods: High-performance thin layer chromatography (HPTLC) often serves as a method for quantification of major withanolides. In the present study, methanolic withanolide extract of in vitro cultured W. coagulans root tissue were carried out using HPTLC. The HPTLC

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Langa-Lomba, Natalia, Laura Buzón-Durán, Eva Sánchez-Hernández, et al. "Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside." Plants 10, no. 7 (2021): 1363. http://dx.doi.org/10.3390/plants10071363.

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Silybum marianum (L.) Gaertn, viz. milk thistle, has been the focus of research efforts in the past few years, albeit almost exclusively restricted to the medicinal properties of its fruits (achenes). Given that other milk thistle plant organs and tissues have been scarcely investigated for the presence of bioactive compounds, in this study, we present a phytochemical analysis of the extracts of S. marianum capitula during the flowering phenological stage (stage 67). Gas chromatography–mass spectroscopy results evidenced the presence of high contents of coniferyl alcohol (47.4%), and secondari

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Rabehaja, Delphin J. R., Harilala Ihandriharison, Panja A. R. Ramanoelina, et al. "Chemical Composition of the Essential Oil from Croton kimosorum, an Endemic Species to Madagascar." Natural Product Communications 9, no. 1 (2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900136.

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Croton kimosorum Leandri is an endemic species to Madagascar. The chemical composition of aerial parts, leaf and stem oils is reported for the first time. Analysis was carried out by combination of chromatographic (CC, GC), spectroscopic and spectrometric (MS, 13C NMR) techniques. In total, 76 compounds have been identified. Essential oil isolated from aerial parts contained mainly linalool (21.6%), sabinene (10.4%), 1,8-cineole (6.3%), β-pinene (6.2%), ( E)-P-caryophyllene (5.9%), terpinen-4-ol (4.8%), geraniol (4,5%) and germacrene D (2.3%). In comparison with the first sample, the compositi

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25

Catanzaro, Daniela, Raffaella Filippini, Caterina Vianello, Maria Carrara, Eugenio Ragazzi, and Monica Montopoli. "Chlorogenic Acid Interaction with Cisplatin and Oxaliplatin: Studies in Cervical Carcinoma Cells." Natural Product Communications 11, no. 4 (2016): 1934578X1601100. http://dx.doi.org/10.1177/1934578x1601100421.

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The antiproliferative effect of the naturally occurring polyphenol chlorogenic acid (CGA) was evaluated in combination with either cisplatin or oxaliplatin in human cervical carcinoma cell lines that were either sensitive (A431) or resistant to cisplatin (A431Pt), in order to provide evidence to overcome drug resistance. Cytotoxicity of platinating drugs (IC50 ~ 10−6 - 10−5 M) was enhanced by 1–2 orders of magnitude by increasing incubation times (1, 4, and 24 hours) in the two cell lines. CGA treatment presented low cytotoxicity per se (IC50 ~ 10−4 M at 24 h) if compared with platinum drugs a

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Abd-ElGawad, Ahmed M., Abdelbaset M. Elgamal, Yasser A. EI-Amier, Tarik A. Mohamed, Abd El-Nasser G. El Gendy, and Abdelsamed I. Elshamy. "Chemical Composition, Allelopathic, Antioxidant, and Anti-Inflammatory Activities of Sesquiterpenes Rich Essential Oil of Cleome amblyocarpa Barratte & Murb." Plants 10, no. 7 (2021): 1294. http://dx.doi.org/10.3390/plants10071294.

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The integration of green natural chemical resources in agricultural, industrial, and pharmaceutical applications allures researchers and scientistic worldwide. Cleome amblyocarpa has been reported as an important medicinal plant. However, its essential oil (EO) has not been well studied; therefore, the present study aimed to characterize the chemical composition of the C. amblyocarpa, collected from Egypt, and assess the allelopathic, antioxidant, and anti-inflammatory activities of its EO. The EO of C. amblyocarpa was extracted by hydrodistillation and characterized via gas chromatography–mas

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Yapi, Thierry Acafou, Jean Brice Boti, Antoine Coffy Ahibo, et al. "Composition and Chemical Variability of Ivoirian Xylopia staudtii Leaf Oil." Natural Product Communications 10, no. 6 (2015): 1934578X1501000. http://dx.doi.org/10.1177/1934578x1501000666.

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The chemical composition of a leaf oil sample from Ivoirian Xylopia staudtii Engler & Diels (Annonaceae) has been investigated by a combination of chromatographic [GC(RI)] and spectroscopic (GC-MS, 13C NMR) techniques. Thirty-five components that accounted for 91.8% of the whole composition have been identified. The oil composition was dominated by the furanoguaiadienes furanoguaia-1,4-diene (39.0%) and furanoguaia-1,3-diene (7.5%), and by germacrene D (17.5%). The composition of twelve other leaf oil samples demonstrated qualitative homogeneity, but quantitative variability. Indeed, the c

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Luận, Vũ Quốc, Nguyễn Bá Nam, Vũ Thị Hiền, et al. "Influence of culture vessel volume and ventilation on in vitro culture and adenosine accumulation of Anoectochilus setaceus Blume." Vietnam Journal of Biotechnology 15, no. 2 (2018): 307–17. http://dx.doi.org/10.15625/1811-4989/15/2/12347.

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Anoectochilus setaceus is a medicinal plant used as a traditional medicine as well as nutritious products for health care in China and many Asian countries. In conventional propagation, the the vessel type, material and volume lead to differences in the moisture content between the internal and external conditions which result in great impact on the quality of in vitro plantlets as well as subsequent growth at nursery. In this study, several factors affecting the multiplication and regeneration of A. setaceus cultured in vitro were investigated. The highest percentage of shoot regeneration (52

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Junairiah, Junairiah, Nurul Sofi Amalia, Yosephine Sri Wulan Manuhara, Ni’matuzzahroh Ni’matuzahroh, and Lilis Sulistyorini. "PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM (Piper betle L. Var Nigra)." Jurnal Kimia Riset 4, no. 2 (2019): 121. http://dx.doi.org/10.20473/jkr.v4i2.16898.

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AbstrakPiper betle L. var Nigra (sirih hitam) merupakan salah satu jenis tanaman obat yang berpotensi untuk dikembangkan dengan metode kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh variasi zat pengatur tumbuh Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin terhadap senyawa metabolit sekunder yang terdapat kalus sirih hitam. Eksplan daun dari sirih hitam ditanam pada medium Murashige dan Skoog dengan 4 perlakuan kombinasi (I1,0K1,0; I1,0K1,5; I1B1,5; I0,5B0,5). Kalus dipelihara selama 8 minggu. Simplisia kalus sirih hitam di maserasi menggunakan pelarut metano

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Rastogi, Subha, Antariksha Katara, Madan M. Pandey, Sumit Arora, R. R. B. Singh, and A. K. S. Rawat. "Physical Stability and HPLC Analysis of Indian Kudzu (Pueraria tuberosaLinn.) Fortified Milk." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/368248.

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Functional foods provide health benefit beyond basic nutrition. Functional foods fortified with plant ingredients are well known. Ayurveda (Indian System of Medicine) has found several ways in which the medicinal benefits of herbs can be conveyed via certain foods as carriers. Milk is one such carrier which has been effectively used to deliver phytochemicals for targeted health benefits. Indian Kudzu orPueraria tuberosaLinn. (Fabaceae) is an important medicinal plant of Ayurveda, and experiments suggest that it enhances the health benefits of milk when taken with milk as a carrier. Different m

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Rai, Preeti, and Harsha Chatrath. "THE COMPARISON OF GC-MS DATA OF LEAVES OF ALOE VERA (A. BARBADENSIS MILL) PLANT GROWN IN DIFFERENT SOIL COMBINATIONS WITH CHEMICAL LAB WASTES." Green Chemistry & Technology Letters 6, no. 2 (2021): 25–30. http://dx.doi.org/10.18510/gctl.2020.623.

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Purpose of the study: The main purpose of this study is to do the comparison of the chemical constituents present in the leaves of Aloe Vera (A. barbadensis Mill) plant grown in different soil combinations using Gas Chromatography- Mass Spectrometry (GC-MS) data analysis. Plants were grown in two different combinations of soil, one in normal soil and the other in the combination of normal soil and solid chemical waste obtained from the chemistry laboratory of an educational institute. Methodology: One Aloe Vera (A. barbadensis Mill) plant was grown in normal soil without adding any compo

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Fabri, Rodrigo Luiz, Danielle Maria de Oliveira Aragão, Jônatas Rodrigues Florêncio, et al. "Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant SpeciesMitracarpus frigiduson AdultSchistosoma mansoniWorms." BioMed Research International 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/941318.

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The aims of this work were to evaluate thein vitroandin vivoschistosomicidal properties of the methanolic extract of the aerial parts ofMitracarpus frigidus(MFM) and to determine its HPLC profile. For thein vitroexperiment, four pairs of adult worms, obtained from infected mice, were exposed to different concentrations of MFM (100 to 400 μg/mL) for 24 and 48 h and analyzed under an inverted microscope. For thein vivoexperiment, mice were inoculated with cercariae and, 20 days after infection, MFM (100 and 300 mg/kg) was administered orally for the following 25 days. Mice were euthanized after

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Xiong, Yao Kun, Gen Hua Zhu, Ju Quan Zhang та ін. "Puriﬁcation of Ferulic Acid, Senkyunolide I and Senkyunolide H from the Volatile Oil of Rhizoma chuanxiong Using High-Speed Countercurrent Chromatography and Preparative Liquid Chromatography". Advanced Materials Research 1033-1034 (жовтень 2014): 259–64. http://dx.doi.org/10.4028/www.scientific.net/amr.1033-1034.259.

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A high-speed countercurrent chromatography and preparative liquid chromatography method was successfully applied to the separation and puriﬁcation of ferulic acid, senkyunolide I and senkyunolide H from the volatile oil of the traditional Chinese medicinal plant Rhizoma Chuanxiong. For the first time, using a two-phase solvent system composed of N-butanol–acetic acid–water (4:1:5, v/v) and a flow rate of 2 mL·min-1, a mixture of SEI and SEH was rapidly obtained by high-speed countercurrent chromatography (HSCCC). The mixture was separated by pre-HPLC with a methanol–water (50:50) mobile phase

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Bubueanu, Corina, Ioana Nicu, and Lucia Pirvu. "Chromatographic Fingerprint Analysis and Bioactivity of Origanum Vulgare Extracts." Malaysian Journal of Medical and Biological Research 3, no. 2 (2016): 95–98. http://dx.doi.org/10.18034/mjmbr.v3i2.413.

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A very important measure for any country, in terms of public health problems and commercial exploitation, must be the development of medicinal and aromatic plant resources. Long-term strategies for the conservation of medicinal and aromatic plants, personnel training, cultivation or passage in culture of valuable species, sustainable methods of harvesting and processing plants to promote scientific research and validation of traditional remedies are needed. In the present, the natural compounds research are mainly focused on their role in improving health. The extraction processes of the natur

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Yeo, Hyeon Ji, Chang Ha Park, Kyoung Bok Lee, et al. "Metabolic Analysis of Vigna unguiculata Sprouts Exposed to Different Light-Emitting Diodes." Natural Product Communications 13, no. 10 (2018): 1934578X1801301. http://dx.doi.org/10.1177/1934578x1801301029.

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In order to determine the effect of light-emitting diodes (LEDs) on plant metabolism, the present study examined the primary and secondary metabolite profiles of Vigna unguiculata L. Walp. sprouts that were exposed to red, blue, white, or a combination of red and blue LEDs using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). A total of 39 hydrophilic compounds were identified and quantitated using GC-TOF-MS, and six phenylpropano

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Kuzmenko, A. N., I. I. Krasnyuk, and A. V. Pirogov. "An algorithm for selecting marker substances in gas chromatographic analysis of medicinal-plant raw materials." Moscow University Chemistry Bulletin 69, no. 4 (2014): 163–67. http://dx.doi.org/10.3103/s0027131414040051.

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Thella, Prabhat Kumar, and V. Ulagamuthalvi. "A Comparative Analysis on Machine Learning Models for Accurate Identification of Medical Plants." Revista Gestão Inovação e Tecnologias 11, no. 4 (2021): 2678–702. http://dx.doi.org/10.47059/revistageintec.v11i4.2309.

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Plants are essential for human life. They help people breathe, provide food, clothing, medicine, and fuel, and also safeguard the environment. Plants can be loaded with medicinal properties and possess active substances that can be used for medical purposes. Several beneficial plant species are disappearing as a result of such factors as global warming, increasing population, professional secrecy, insufficient government support for research efforts, and the lack of public understanding of medicinal plants. It takes time to identify medicinal plants, therefore use professionals to assist you.

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Nyiredy, Szabolcs. "The Role of Planar Chromatography in Medicinal Plant Research." Journal of AOAC INTERNATIONAL 84, no. 4 (2001): 1219–31. http://dx.doi.org/10.1093/jaoac/84.4.1219.

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Abstract This paper summarizes the role of planar chromatography (PC) in medicinal and aromatic plant (MAP) research and development, and demonstrates the importance of the technique, after extraction, in the analysis of MAP (identification and quantitative determination of the separated compound/s), in the purification and isolation process, and in different types of screening procedure. Special attention is paid to analytical, micropreparative and preparative forced-flow techniques, for example overpressured-layer chromatography (OPLC) and rotation planar chromatography (RPC). The special fe

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Kongkiatpaiboon, Sumet, Vichien Keeratinijakal, and Wandee Gritsanapan. "HPLC Analysis of Stemokerrine and Oxystemokerrine in the Thai Medicinal Plant Stemona kerrii." Natural Product Communications 7, no. 10 (2012): 1934578X1200701. http://dx.doi.org/10.1177/1934578x1200701007.

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A HPLC-UV method was developed and validated for determination of stemokerrine and oxystemokerrine in Stemona kerrii roots. The chromatographic separation was performed on a Hypersil BDS C18-column eluted with methanol: 50 mM ammonium acetate solution using a gradient system with a flow rate of 1 mL/min and detection at 300 nm. Stemokerrine and oxystemokerrine showed a linear relationship within the range of 0.5-100 μg/mL. The method was shown to be precise with a RSD <2%. The average percent recovery of stemokerrine was 101.6% and for oxystemokerrine 99.5%. Two samples of S. kerrii were an

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Gomaa Darwish, Ahmed Gomaa, Mamdouh Nabil Samy, Sachiko Sugimoto, Hideaki Otsuka, and Katsuyoshi Matsunami. "A New Macrolactone, Racemolide Along With Seven Known Compounds With Biological Activities From Mangrove Plant,Lumnitzera racemosa." Natural Product Communications 14, no. 6 (2019): 1934578X1986125. http://dx.doi.org/10.1177/1934578x19861255.

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One new macrolide, racemolide (1), was isolated from an MeOH extract of the leaves of Lumnitzera racemosa with 7 known compounds (2-8) by various chromatographic techniques via silica gel, octadecylsilyl column chromatography and high-performance liquid chromatography. The structure of the new compound was determined through a combination of spectroscopic and chemical analyses. The isolated compounds were evaluated for their antileishmanial, hepatoprotective, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Compound 1 showed potent antileishmanial activity at inhibition

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Vincent, Delphine, Simone Rochfort, and German Spangenberg. "Optimisation of Protein Extraction from Medicinal Cannabis Mature Buds for Bottom-Up Proteomics." Molecules 24, no. 4 (2019): 659. http://dx.doi.org/10.3390/molecules24040659.

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Medicinal cannabis is used to relieve the symptoms of certain medical conditions, such as epilepsy. Cannabis is a controlled substance and until recently was illegal in many jurisdictions. Consequently, the study of this plant has been restricted. Proteomics studies on Cannabis sativa reported so far have been primarily based on plant organs and tissues other than buds, such as roots, hypocotyl, leaves, hempseeds and flour. As far as we know, no optimisation of protein extraction from cannabis reproductive tissues has been attempted. Therefore, we set out to assess different protein extraction

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Miladinović, Dragoljub L., Budimir S. Ilić, Tatjana M. Mihajilov-Krstev, Jovana L. Jović, and Marija S. Marković. "In vitro Antibacterial Activity of Libanotis montana Essential Oil in Combination with Conventional Antibiotics." Natural Product Communications 9, no. 2 (2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900238.

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The chemical composition and antibacterial activity were examined of Libanotis montana Crantz subsp. leiocarpa (Heuff.) Soó. (Apiaceae) essential oil. Gas chromatography and gas chromatography/mass spectrometry were used to analyze the chemical composition of the oil. The antibacterial activity was investigated by the broth microdilution method against thirteen bacterial strains. The interactions of the essential oil and three standard antibiotics: tetracycline, streptomycin and chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combinatio

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Juszczak, Zovko-Končić, and Tomczyk. "Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives." Biomolecules 9, no. 11 (2019): 731. http://dx.doi.org/10.3390/biom9110731.

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Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quan

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Botrel, P. P., M. A. Rodrigues, S. V. Bertolucci, A. F. Lima, I. C. A. Alvarenga, and J. P. Pinto. "FACTORS AFFECTING IN VITRO PROPAGATION AND CHROMATOGRAPHIC ANALYSIS OF COMPOUNDS IN HYPTIS MARRUBIOIDES EPL., A THREATENED MEDICINAL PLANT." Acta Horticulturae, no. 1083 (May 2015): 319–25. http://dx.doi.org/10.17660/actahortic.2015.1083.40.

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Durón, Rosalba Ramírez, Luca Ceniceros Almaguer, Norma Cecilia Cavazos Rocha, Perla Giovanna Silva Flores, and Noem Waksman De Torres. "Comparison of High-Performance Liquid Chromatographic and Thin-Layer Chromatographic Methods for Determination of Aloin in Herbal Products Containing Aloe vera." Journal of AOAC INTERNATIONAL 91, no. 6 (2008): 1265–70. http://dx.doi.org/10.1093/jaoac/91.6.1265.

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Abstract Aloe vera is a medicinal plant used worldwide to treat a variety of conditions and, as such, has important commercial value. Aloin is a principal component of aloe vera leaves and is used for quality control of products containing it. A semiquantitative thin-layer chromatographic (TLC) method for determining the concentration of aloin in aloe-based products was validated. The results were similar to those of a validated high-performance liquid chromatographic method; therefore, TLC, which is a simple, sensitive, specific, rapid, and cheap method, may be ideal for use in any laboratory

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CLAUDINO, JOSIANE C., LUIS V. S. DO SACRAMENTO, INGRID KOCH, et al. "Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz." Anais da Academia Brasileira de Ciências 85, no. 4 (2013): 1253–65. http://dx.doi.org/10.1590/0001-3765201393812.

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Casearia sylvestris Swartz (Salicaceae) has been used in traditional medicine and its leaf extracts have been exhibited important pharmacological activities. The species presents morphological, chemical and genetic variation. Two varieties are considered due external morphological differences: C. sylvestris var. sylvestris and var. lingua. There are difficulties in definition of these varieties. The objective of this work is to evaluate chemical and morpho-anatomical differences between C. sylvestris varieties that can be applied in their distinction for pharmaceutical or botanical purposes. T

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Balogun, O. S., I. A. Oladosu, and Zhiqiang Liu. "Isolation of 2, 5-dithia-3, 6-diazabicyclo [2.2.1] heptane and GC-MS analysis of silylated extract from Tragia benthamii." Ife Journal of Science 22, no. 2 (2020): 075–80. http://dx.doi.org/10.4314/ijs.v22i2.7.

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Tragia benthamii is a medicinal plant of repute endemic in West Africa. However, despite the traditional uses of the plant its secondary metabolites are yet to be profiled. Thus, this study aimed at identifying the chemical constituents of T. benthamii. Air-dried samples (600 g) of the plant were extracted with aqueous ethanol (95 %) and thereafter, portions of the crude extract obtained were used separately for chromatographic purifications, GC-MS analysis and spectrophotometric quantitation of the secondary metabolites. The chromatographic purification of the extract afforded a pure compound

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Jirovetz, Leopold, Gerhard Buchbauer, Christiane Puschmann, Wilhelm Fleischhacker, P. Mohamed Shafi, and M. K. Rosamma. "Analysis of the Essential Oil of the Aerial Parts of the Medicinal Plant Aristolochia indica Linn. (Aristolochiaceae) from South-India." Scientia Pharmaceutica 68, no. 3 (2000): 309–16. http://dx.doi.org/10.3797/scipharm.aut-00-28.

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The essential oil of the aerial parts of Aristolochia indica Linn. (Anstolochiaceae) from South-India was analyzed by gas chromatographic-spectroscopic (GC-FID and GC-MS) and olfactoric methods to identify those compounds responsible for the characteristic odor as well as partly for the folk medicinal use of this plant. Especially sesqui- and monoterpenes were found to be dominating constituents of this essential oil, such as: β-caryophyllene, α-humulene, ishwarone, caryophyllene oxide I, ishwarol, ishwarane and aristolochene as well as linalool and α-terpinolene.The odor impression of the sam

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Mazivila, Sarmento J., Joaquim C. G. Esteves da Silva, Ricardo N. M. J. Páscoa, and João M. M. Leitão. "Simultaneous Determination of Medicinal Drugs with Overlapping Profiles Contained in Low Chromatographic Resolution Data using HPLC-DAD and Multivariate Curve Resolution." Current Analytical Chemistry 16, no. 7 (2020): 843–53. http://dx.doi.org/10.2174/1573411015666191104155725.

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Background: The increasing demand of effective pharmaceutical products directed to fight against malaria lead to the combination of at least two antimalarial drugs. This combination aims to minimize the Plasmodium falciparum resistance which is found when the most frequently used drugs are taken individually. Within this context, proguanil hydrochloride and chloroquine phosphate which have independent modes of action are taken together to prevent malaria. This paper aims to develop a fast and powerful analytical method for the simultaneous determination of proguanil hydrochloride and chloroqui

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Behzadifar, Faegheh, Sayeh Marandi, Ahmad Majd, Fahimeh Salimpour, and Sedigheh Arbabian. "Genetic diversity analysis of the medicinal plant Heracleum persicum desf. ex fischer." Genetika 53, no. 2 (2021): 757–68. http://dx.doi.org/10.2298/gensr2102757b.

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Persian hogweed (Heracleum persicum Desf. ex Fischer) is native to Iran but was introduced to Europe as an invasive plant. It has medicinal and edible values. Its fruits have been used to relieve flatulence, stomach aches as well as a flavoring as a digestive and an antiseptic. This medicinal plant forms many geographical populations in the country, particularly in mountainous regions; however, we have no data on the genetic structure and genetic diversity of this plant species. Such information can be used in gene pool identification and future genetic conservation and breeding programs. Ther

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