Relevant bibliographies by topics / Medicinal plants (In vitro study)

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Academic literature on the topic 'Medicinal plants (In vitro study)'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 February 2022

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Journal articles on the topic "Medicinal plants (In vitro study)"

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Adukpo, Selorme, Doris Elewosi, Richard Harry Asmah, Alexander K. Nyarko, Patrick Kwaku Ekpe, Dominic Adotei Edoh, and Michael Fokua Ofori. "Antiplasmodial and Genotoxic Study of Selected Ghanaian Medicinal Plants." Evidence-Based Complementary and Alternative Medicine 2020 (September 23, 2020): 1–10. http://dx.doi.org/10.1155/2020/1582724.

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Ethnopharmacological Relevance. Development of resistance to antimalarial drugs by Plasmodium falciparum is still rampant, and there is an urgent need for novel drugs to either standalone or to partner artemisinin for treatment of malaria. Traditionally, plants have, over the years, been a good source of antimalarial drugs. Efficacy and safety of such plants need to be scientifically authenticated. Aims, Materials, and Method. This study investigated the in vitro antiplasmodial activity, cytotoxicity, and genotoxicity of aqueous extracts of Acanthospermum hispidum DC, Alstonia boone (De Wild), Cocos nucifera L, Cymbopogon citratus (DC.) Stapf, Morinda lucida Benth, Psidium guajava, Phyllanthus niruri L, and Senna siamea Lam. Results. Five out of the eight plants, A. boonei stem bark, S; siamea Lam root, M. lucida Benth leaves, P. niruri, and A. hispidum DC whole plants, showed varying degrees of antiplasmodial activity against the asexual stage of the parasite. The most active extract against chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) P. falciparum strains is the A. hispidum extract which yielded a mean inhibitory concentration at 50% (IC50) of 3.66 µg/ml and 3.71 µg/ml for 3D7 and Dd2, respectively. This was followed by S. siamea Lam with 3.95 µg/ml for 3D7 and 4.47 µg/ml for Dd2. The IC50 values of the A. boonei extract against 3D7 and Dd2 P. falciparum parasites were 5.13 µg/ml and 3.62 µg/ml, respectively. For the M. lucida Benth extract, the least IC50 value was 6.46 µg/ml. All five extracts exhibited dose-dependent antiplasmodial activity. Assessment of the genotoxic effects the A. hispidum extract by the comet assay revealed substantial damage to P. falciparum DNA. Conclusion. This study demonstrates that the crude extract of A. hispidum DC, one of the plants used traditionally to treat malaria, inhibits the growth of P. falciparum in vitro and could be a potential source of antimalarial drug. The report has highlighted genotoxic and cytotoxic effects of the selected plant extracts on human leukocytes as well.
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U, Gowthama Prabu, Anushri I, Kishor Kumar B, Saravanan M, and Baskar R. "Study on the in vitro antioxidant properties of selected traditional medicinal plants." Journal of Pharmacognosy and Phytochemistry 9, no. 4 (July 1, 2020): 1831–37. http://dx.doi.org/10.22271/phyto.2020.v9.i4y.12023.

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Kalekar, SamidhaA, RenukaP Munshi, SupriyaS Bhalerao, and UrmilaM Thatte. "Insulin sensitizing effect of 3 Indian medicinal plants: An in vitro study." Indian Journal of Pharmacology 45, no. 1 (2013): 30. http://dx.doi.org/10.4103/0253-7613.106431.

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Rengarajan, Sumathy, Vijayalakshmi Melanathuru, Deecaraman Munuswamy, Sankaranarayanan Sundaram, and Saravanan Thiruverkadu Selvaraj. "A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS." Asian Journal of Pharmaceutical and Clinical Research 9, no. 5 (September 1, 2016): 259. http://dx.doi.org/10.22159/ajpcr.2016.v9i5.13476.

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ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.
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Waller, Stefanie Bressan, Isabel Martins Madrid, Renata Osório de Faria, Marlete Brum Cleff, João Roberto Braga de Mello, and Mário Carlos Araújo Meireles. "Anti- Sporothrix spp. activity of medicinal plants." Brazilian Journal of Pharmaceutical Sciences 52, no. 2 (June 2016): 221–37. http://dx.doi.org/10.1590/s1984-82502016000200001.

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ABSTRACT Cases of sporotrichosis in humans and animals without satisfactory clinical response have increased, a warning sign of strains resistant to conventional antifungal agents. The urgent search for alternative therapies was an incentive for research on medicinal plants with anti-Sporothrix spp. properties. A bibliographic survey was performed based on scientific papers about in vitro and in vivo antifungal activity of essential oils and extracts of plants in differents solvents against the fungal of the Sporothrix schenckii complex. The study methodology consisted of a literature review in Google Scholar, Science Direct, Pubmed, Bireme and Springer link with papers from 1986 to 2015. We found 141 species of plants that were investigated, of which 100 species were concentrated in 39 botanical families that had confirmed anti-Sporothrix activity. Combretaceae, Asteraceae and Lamiaceae represented the botanical families with the greatest number of plants species with antifungal potential, using different methodologies. However, there are few studies with medicinal plants in experimental infection in animals that prove their activity in the treatment of sporotrichosis. It reinforces the need for further research related to standardization of in vitro methodologies and in vivo studies related to safety and to toxicity potential of these plants with anti-Sporothrix spp. activity.
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Chiang, Lien-Chai, Hua-Yew Cheng, Chi-Chain Chen, and Chun-Ching Lin. "In vitro Anti-leukemic and Antiviral Activities of Traditionally Used Medicinal Plants in Taiwan." American Journal of Chinese Medicine 32, no. 05 (January 2004): 695–704. http://dx.doi.org/10.1142/s0192415x04002284.

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Medicinal plants have been historically used as treatment for different kinds of human diseases. In this study, hot water (HW) extract of five Taiwanese traditionally used medicinal plants was evaluated for their in vitro anti-leukemic (including anti-K562, L1210, P3HR1, Raji and U937 leukemia cells) and antiviral (including HSV-1 and HSV-2) activities. Results showed that Blumea lacera exhibited broad anti-leukemic activity at magnitudes ranging from moderate to mild and Ixeris chinensis is effective at inhibiting the proliferation of K562 cells. B. lacera and Tithonia diversifolia suppressed the replication of HSV-1 and HSV-2, and had IC 50 values below 100 μg/ml. The medicinal plants showed no cytotoxic effect at concentrations that inhibited HSV infection. It was, therefore, concluded that the HW extract of tested medicinal plants exhibited anti-leukemic and antiviral activities at different magnitudes of potency.
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Robert, P. Ethalsha, and A. Malar Retna. "QUANTITATIVE PHYTOCHEMICAL DETERMINATIONS AND IN-VITRO CYTOTOXIC ACTIVITY OF MEDICINAL PLANTS." Green Chemistry & Technology Letters 1, no. 01 (November 1, 2015): 17–21. http://dx.doi.org/10.18510/gctl.2015.113.

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The present study evaluates in-vitro cytotoxic activity of active extracts of medicinal plants. It evaluates anti-cancer activity of most active extracts of I. pes-caprae and C. roseus. This study focuses on the total phenol content and the total flavanoid content of the plant and searches into its medicinal potency based on the total phenol and total flavanoid content. The anti-cancer activity of the extract is found to be more significant than one another.The cytotoxic activity of Ipomoea pes-caprae and C. roseus showed better results. Methanol extracts of aerial and root of Ipomoea pes-caprae possess maximum anticancer activity is found to be 61.77% and 65.55%. Chloroform extract of Catharanthus roseus root was also active against MCF- 7 cells by exhibiting 64.34% of activity. These plants may be a source of new antibiotic compounds.These findings enriches our knowledge of the chemical constituents that are responsible for the medicinal uses of the plant and the anti-cancer potential of selected plants.
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Rizvi, Tania Shamim, Abdul Latif Khan, Liaqat Ali, Narjis Al-Mawali, Fazal Mabood, Javid Hussain, Muhammad Adnan, and Ahmed Al-Harrasi. "In vitro oxidative stress regulatory potential of Citrullus colocynthis and Tephrosia apollinea." Acta Pharmaceutica 68, no. 2 (June 1, 2018): 235–42. http://dx.doi.org/10.2478/acph-2018-0012.

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Abstract The present study investigates the potential role of medicinal plants Citrullus colocynthis and Tephrosia apollinea in ameliorating the oxidative stress developed during the generation of reactive oxygen species. Organic extracts of different organs (leaf, stem and root) of these medicinal plants obtained in n-hexane, chloroform, n-butanol and water were assayed for radical scavenging, total antioxidant capacity, anti-lipid peroxidation and reduced glutathione. The total phenolic content (TPC) of both selected medicinal plants was also evaluated. The results indicated that extracts of T. apollinea leaf, stem and root have higher TPC compared to those of C. colocynthis. Similarly, the results of the present study revealed higher bioactivity of C. colocynthis than that of T. apollinea in various antioxidant assays. Various plant parts of each plant were also compared.
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Dossymbetova, Symbat, Aigul Amirova, Taira Kurbangaliyeva, Khalima Abdresh, and Gulzhan Kurtibayeva. "Germination of seeds of medicinal plants Origanum vulgare L. and Salvia officinalis L. in vitro." Bulletin of the Karaganda University. “Biology, medicine, geography Series” 102, no. 2 (June 30, 2021): 23–29. http://dx.doi.org/10.31489/2021bmg2/23-29.

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The problem of biodiversity conservation and rational use of natural resources remains one of the most pressing problems for countries around the world. To maintain the stability of biological resources a sufficiently developed basis for their reproduction is required, which, in turn requires a preliminary study of the developmental features of individual organisms both in the natural environment and during their introduction into the cultural environment. A special place among the spice plants is occupied by the Lamiaceae Lindl. family. It is rich in medicinal and essential oil plants, which are widely used in family medicine, as well as in folk medicine and food industry, perfumery and cosmetic industries. Such plants include Origanum vulgare and Salvia officinalis. In addition, both plant species are added as phytobiotics to animal feed. The article deals with sterilization of seeds of medicinal plants Origanum vulgare L. and Salvia officinalis L. and the effect of sterilization methods on seed germination. For in vitro cultivation an aseptic, undamaged pure culture was obtained from the original plant material, and the most optimal method for sterilizing the seeds of these medicinal plants was chosen.
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Almeida, Carolina Medeiros, Eveline Angélica Lira de Sousa Sales Rocha, Érika Ponchet Alves, Rennaly De Freitas Lima, Yuri Wanderley Cavalcanti, Raquel Christina Barbosa Gomes, Jozinete Vieira Pereira, and Edja Maria Melo de Brito Costa. "In vitro Evaluation of the Antimicrobial Potential of Salvia officinalis L. against Oral Pathogens." Journal of Health Sciences 21, no. 2 (June 19, 2019): 129. http://dx.doi.org/10.17921/2447-8938.2019v21n2p129-133.

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AbstractThe emergence of multidrug-resistant strains to antibiotics has motivated the search for new substances with antimicrobial activity, especially those derived from medicinal plants. Salvia officinalis L. is a medicinal plant that arouses scientific interest due to being associated with multiple therapeutic effects. The purpose of this study was to evaluate the in vitro antimicrobial potential of S. officinalis L. against pathogens in the oral cavity. The antimicrobial potential of the ethanol extract of leaf of S. officinalis L was evaluated by broth microdilution, with determination of minimum inhibitory concentration (MIC) and Minimum bactericidal/Fungicide concentration (MBC / MFC), against the species Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei and Candida tropicalis. The extract showed moderate antifungal potential before Candida species (MIC = 1 mg/mL). And for the species of Streptococcus, the antimicrobial activity was from moderate to strong whose MIC ranged from 0.25 to 1 mg/mL. In this study, the extract from the leaves of S. officinalis L. presented oral cavity antimicrobial activity against pathogens. These results point to S. officinalis as a possible source of active ingredients in the development of formulations with antimicrobial activity of dental use. Keywords: Plants, Medicinal. Salvia officinalis L. Products with Antimicrobial action.ResumoO surgimento de cepas multirresistentes a antibióticos tem motivado a busca de novas substâncias com atividade antimicrobiana, especialmente aquelas oriundas de plantas medicinais. A Salvia officinalis L. é uma planta medicinal que desperta interesse científico por estar associada a múltiplos efeitos terapêuticos. O objetivo do presente estudo foi avaliar o potencial antimicrobiano in vitro da S. officinalis L. frente a patógenos da cavidade bucal. O potencial antimicrobiano do extrato etanólico da folha da S. officinalis L foi avaliado por meio da microdiluição em caldo, com determinação da Concentração Inibitória Mínima (CIM) e Concentração Bactericida/Fungicida Mínima (CBM/CFM), frente as espécies Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei e Candida tropicalis. O extrato apresentou moderado potencial antifúngico frente às espécies de Candida (CIM = 1 mg/mL). Para as espécies de Streptococcus, o potencial antimicrobiano foi considerado forte a moderado, com valores de CIM variando entre 0,25 a 1 mg/mL. Neste estudo, o extrato da folha de S. officinalis L. apresentou potencial antimicrobiano contra patógenos da cavidade bucal. Esses resultados apontam a S. officinalis como uma possível fonte de princípios ativos no desenvolvimento de formulações com atividade antimicrobiana de uso odontológico. Palavras-chave: Plantas Medicinais. Salvia officinalis L. Produtos com Ação Antimicrobiana.
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Dissertations / Theses on the topic "Medicinal plants (In vitro study)"

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Odeyemi, Samuel Wale. "A comparative study of the in vitro antidiabetic properties, cytotoxicity and mechanism of action of Albuca bracteata and Albuca setosa bulb extracts." Thesis, University of Fort Hare, 2015. http://hdl.handle.net/10353/3154.

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The search for cheap, non toxic and readily available antidiabetic drugs has been a challenge for researchers and the pharmaceutical industries. Diabetes mellitus is a metabolic disease characterized by defects in the synthesis of insulin and/or insensitivity to the action of insulin at the target cells. The disease has been on the increase mostly in developing countries where large proportions of the population have little access to good medical care due to either accessibility or non availability of synthetic drugs. This has led to the use of medicinal plants to treat diabetes because it is safe, cheap and with few side effects. There is little scientific evidence on the dosages, active compounds, mechanisms of action and toxicity of these traditionally used plants. Two of the most frequently used plants; Albuca setosa and Albuca bracteata were investigated in this study. The qualitative analysis of different extractions of these plants revealed the presence of phenolics, alkaloids, tannins and saponins. The antioxidant properties of aqueous, acetone and methanollic extracts of Albuca setosa and Albuca bracteata were investigated using models such as Diphenyl-1-Picrylhydrazyl (DPPH), 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Ferric ion reducing antioxidant potential (FRAP), Nitric Oxide and Hydrogen Peroxide (H2O2). Both plants revealed inhibitions against DPPH in a concentration - dependent manner with Albuca setosa (0.330 mg/ml) showing higher activity than Albuca bracteata (0.647 mg/ml) determined from the IC50. The aqueous extract of Albuca setosa showed a higher inhibition against DPPH radical compared to the Albuca bracteata aqueous extract at all concentrations investigated. The isolated saponins from Albuca bracteata had a higher DPPH scavenging activity than the crude methanolic extract of the plant in a concentration - dependent manner but are significantly different from each other at 0.4, 0.6 and 1.0 mg/ml only. The IC50 of the saponins was also observed to be higher than the crude extracts and standards.The Albuca setosa aqueous extract showed a higher percentage inhibition of ABTS radicals than Albuca bracteata at all the concentrations investigated. Overall, the Albuca setosa aqueous extract (0.0809 mg/ml) showed maximum activity against ABTS radicals. The iron reducing power was significantly higher (P < 0.05) in the methanolic extract of both plants compared to the aqueous counterpart. Overall, the Albuca bracteata aqueous extract (0.344 mg/ml) showed maximum activity as indicated by the IC50. The aqueous extracts of both plants also revealed percentage inhibitions in a concentration - dependent manner against NO2. The aqueous extract of Albuca bracteata bulb was more active against nitric oxide and hydrogen peroxide inhibition. In this study, the cytotoxicity of the extracts was evaluated at a high dose of 100 μg/ml on Chang liver cells and determined using MTT, crystal violet, glucose consumption, lactate production and lactate dehydrogenase release and FRAP. The aqueous extracts of both Albuca setosa and Albuca bracteata were non-toxic on Chang liver cells at the concentrations investigated. The MTT revealed that the aqueous extract of Albuca setosa bulb had the optimum cell viability of 108.09 percent while the acetonic extract of Albuca bracteata showed the least cell viability (37.72 percent) compared with the control. The crystal violet test also revealed the acetone extract of Albuca bracteata to have the least percentage of cell viability at 31.47 percent, while the aqueous extract of Albuca setosa showed the maximum cell viability at 112.5 percent. The aqueous extracts of both plants showed higher percentage cell density on the second day of incubation from the proliferation assay. All the tested samples were observed to consume more glucose than the blank except for the methanollic and acetone extracts of Albuca bracteata bulb. The aqueous and methanolic extracts of Albuca setosa bulbs produced the highest lactate with 120.2 μg/ml and 113.7 μg/ml respectively. The acetone extracts of both Albuca setosa and Albuca bracteata revealed toxicity with a higher lactate dehydrogenase release compared to the control.
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Silva, Ana Mara de Oliveira e. "Efeito dos compostos fenólicos presentes no alecrim (Rosmarinus officinalis L.) sobre as enzimas antioxidantes e os parâmetros bioquímicos do sangue de ratos diabéticos induzidos por estreptozotocina (OU) Efeito dos compostos fenólicos presentes no alecrim (Rosmarinus officinalis L.) sobre as enzimas antioxidantes e os parâmetros bioquímicos de ratos diabéticos induzidos por estreptozotocina." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/9/9132/tde-26092017-161242/.

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As ervas e especiarias são fontes de antioxidantes. Essa capacidade antioxidante está relacionada aos compostos fenólicos que apresentam papel importante nos processos de inibição da peroxidação e podem atuar sobre o estresse oxidativo, relacionado com diversas patologias, inclusive o diabetes. O alecrim é bastante apreciado por seu aroma e sabor, tendo como constituintes os seguintes compostos fenólicos: ácido carnósico, carnosol, ácido rosmarínico, ácido caféico e éster do ácido hidroxicinâmico. Objetivou-se avaliar a capacidade antioxidante in vitro de extratos e frações de ácidos fenólicos obtidos das folhas de alecrim e seu efeito sobre ratos diabéticos induzidos por estreptozotocina. Constatou-se que tanto os extratos como as frações apresentaram altos teores de compostos fenólicos totais e expressiva atividade antioxidante in vitro nos três métodos utilizados: β-caroteno/ácido linoléico, varredura do radical DPPH e ORAC. No ensaio in vivo, foi utilizado modelo de diabetes tipo 1, apresentando as características principais: poliúria, polifagia, polidipsia e perda de peso. Observaram-se alterações na atividade das enzimas antioxidantes, nos parâmetros bioquímicos do sangue e aumento significativo (p<0,05) da glicemia, no percentual de hemoglobina glicosilada (Hb-G), nos níveis de triglicérides, de colesterol total, de creatinina, de AL T e de AST. O extrato aquoso de alecrim administrado por 30 dias na concentração de 50 mg/Kg aumentou a atividade das enzimas CAT e GPx, no fígado, e da SOD no cérebro de ratos diabéticos, diminuindo também o percentual de Hb-G. A mesma dose, quando administrada por 60 dias, reduziu o percentual de Hb-G, nos lipídios circulantes, na creatinina, na atividade das enzimas SOD e GPx dos tecidos avaliados e manteve os valores normais das enzimas de função hepática AL T e AST. Não foi observado efeito dose resposta nos parâmetros analisados, sugerindo que a maior dose (100mg/Kg) apresentou níveis de toxicidade que devem ser melhor caracterizados. Portanto, o extrato aquoso de alecrim apresentou capacidade antioxidante in vitro significativa e quando administrado na concentração de 50 mg/Kg pode ter papel importante sobre o estresse oxidativo presente no diabetes experimental.
Herbs and spices are potential sources of antioxidants. This antioxidant capacity is related to the presence of phenolic compounds that play an important role in the peroxidation inhibition processes, that can act on the oxidative stress, which is related to various diseases, including the diabetes. Rosemary is much appreciated due to its aroma and flavor properties, and has the following constituents as phenolic compounds: carnosic acid, carnosol, rosmannlc acid, cafeic acid, and hydroxycinnamic ester. This study aimed to evaluate the in vitro antioxidant capacity of the extracts and fractions of phenolic acids obtained from the leaves of rosemary and its effect on diabetic rats induced by streptozotocin. The results showed that both extract and fractions contains high leveis of total phenolic compounds, and a significant in vitro antioxidative activity evaluated by three methods: β-carotene / Linoleic acid system, DPPH radical scavenging and ORAC. For in vivo tests, it was used a type 1 diabetes model, which presented the main clinicai features: polyuria, polyfagia, polydipsia, and weight loss. It was also observed the following changes in biochemical markers: an altered antioxidant enzymatic activity; a significant increase (p<0.05) of blood glucose, percentage of glycosylated hemoglobin (Hb-G), triglycerides, total cholesterol, creatinine, and enzymes ALT and AST. The aqueous extract of rosemary administered daily for 30 days at dose of 50 mg/kg increased the activity of enzymes CAT and GPx in the liver and SOD in the brain of diabetic rats, also decreased the percentage of Hb-G. The same dose, when administered for 60 days, led to a reduction of percentage of Hb-G, circulating lipids, and creatinine, also reducing the activity of enzymes SOD and GPx in the analyzed tissues, and maintaining the normal function of liver enzymes AL T and AST. There was no dose¬response effect on the studied parameters. Some toxic effect was observed when higher doses were used (100 mg/kg) but this effect must be better characterized. Therefore, aqueous extracts of rosemary at dose of 50 mg/kg presented a significant in vitro antioxidant capacity that can be an important role on the oxidative stress in experimental diabetes.
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Matongo, Kudakwashe. "Conservation and use-values of medicinal plants in rural eastern Zimbabwe: A study of selected medicinal plants." Thesis, University of the Western Cape, 2012. http://hdl.handle.net/11394/4065.

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Magister Artium (Development Studies) - MA(DVS)
Medicinal plants remain a very important natural resource used as traditional medicines for health needs in many developing countries. In the current deepening economic and political crises in Zimbabwe a significant number of the population has inevitably relied more on natural resources which has led to receding population and scarcity of many medicinal plant species in their natural habitat. It is against this background that this research, using Rural Eastern Zimbabwe that this study explored the extent to which use values of medicinal plants increased since the Zimbabwean crises and the different use values of these species among men and women. The rational choice theory, use value approach and concept of utility constituted a theoretical grounding of the research process. The study essentially used qualitative research methods with some quantitative data. A mix of interviews and focus group discussions were employed for this study. Interviews were conducted with community leaders, traditional healers, NGOs in the similar field and Government stakeholders eliciting their views on use values of medicinal plants and sustainable interventions that can be enacted in conserving these species. The findings of the study were shown through using tables, charts and the quantitative data was presented using STATA. The calculated total usevalues of the 11 medicinal plants showed that Kirkia ancuminata Oliv, Dicoma anomala Sond, Syzgium guineense DC, Zingiber offinale, Acacia Karoo Hayne were found to have “high total use-values” and Lannea edulis Engl, Aloe, Lippia javanica Spreng, Virtex payos merril, parinari curatelli and Coleochloa setiflora have “low total use-values”
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Siriwatanametanon, Nisarat. "Traditionally used Thai medicinal plants : in vitro anti-inflammatory, anticancer and antioxidant activities." Thesis, University College London (University of London), 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522827.

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Ntutela, Siyabulela Calvin Sibusiso. "In vitro efficacy tests against Mycobacterium species of South African traditional medicinal plants." Doctoral thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/3445.

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Includes bibliographical references.
Tuberculosis is the leading cause of death due to a single organism; with a mortality of more than 3million people each year, worldwide. The emergence of multi-drug resistance and HIV/AIDS are the major causes of this problem. New therapeutic agents with a different mode of action, and thereby of resistance to Mycobacterium tuberculosis, the causative agent, are needed urgently. Amongst the methods used, the area of ethnopharmacology is explored in this study. Visits were performed to collect the plants used by traditional healers in 7 provinces of South Africa for the treatment of tuberculosis.
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Van, Rooyen Anzel. "The effect of in vitro digestion on selected biological activities of Hypoxis sobolifera corms." Thesis, Nelson Mandela Metropolitan University, 2013. http://hdl.handle.net/10948/d1020058.

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In South Africa part of the cultural and religious beliefs of the African people is the use of traditional remedies to treat diseases. These remedies are obtained from medicinal plants (Steenkamp, 2003). One of the most frequently traded plants in the Eastern Cape is Hypoxis, commonly known as Afrika patat, or African potato. South African traditional healers instruct patients to brew the fresh Hypoxis corm as a tea and then ingest it (Steenkamp, 2006a). This prompted an investigation into the digestive stability of a traditionally prepared Hypoxis extract. The H. sobolifera extracts were digested using a simulated gastric/small intestinal digestion and their biological activity determined. The hot water H. sobolifera extract before digestion only showed cytotoxic activity against cancer cell lines at very high concentrations which are not likely to be achieved under normal ingestion circumstances. In Chang liver cells on the other hand, chronic exposure to the hot water H. sobolifera extract increased glucose uptake in amounts similar to that of metformin. On the negative side, the glucose utilization stimulation was lost due to the simulated digestion process. The significant inhibition of AGEs by hot water H. sobolifera extract (IC50 of 6.3 Ig/ml) is a very encouraging result as treatment in the management of diabetes. This activity was only slightly reduced by the in vitro digestion process. Also observed was enzyme inhibition activity by traditionally prepared H. sobolifera, with ∝-amylase being inhibited (IC50 of approximately 250 Ig/ml) and therefore preventing or limiting starch breakdown. From the DPPH results it was clear that H. sobolifera, even when digested, is a potent anti-oxidant (IC50 of 134.4 Ig/ml when undigested compared to 162.9 when digested with β-glucosidase added to stomach digestive step). HPLC and TLC experiments revealed that rooperol which has previously been thought to be the compound responsible for the anti-oxidant activity in Hypoxis extracts, was absent from the traditional extract of H. sobolifera and therefore cannot be the sole compound exhibiting anti-oxidant activity; other compounds such as phenolics may be contributing. The phenolic and flavonoid content results revealed very highconcentrations of these compounds in the traditionally prepared H. sobolifera extract. These compounds may therefore play major roles in all of the biological activities observed from treatment with Hypoxis spp. The ROS results yielded interesting and promising results. Using standard or traditionally prepared H. sobolifera extracts, activation of differentiated U937 cells with PMA was greatly enhanced by cotreatment with the extracts, while extracts on their own did not cause significant activation. Future studies should investigate this property of the extracts as a promising immune boosterThe HPLC results showed that hypoxoside was undetectable in the hot water traditional extract and the TLC anti-oxidant experiment proved that rooperol is not present in the hot water traditional extract after treatment with β-glucosidase. This indicates that neither one of the Hypoxis compounds previously believed to be responsible for the biological activities observed are present in the extract when prepared the traditional way. Therefore, the biological activities observed in this study can be attributed to other phytochemical compounds.
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Luseba, D., ME Letsoalo, and Katerere. "A comparative study of antibacterial activities of wild and cultivated plants used in ethnoveterinary medicine." African Journal of Biotechnology, 2010. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1000679.

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Farmers generally collect fresh plant materials from the wild for ethnoveterinary uses. They are encouraged to harvest with caution and dry or cultivate important materials in order to protect the biodiversity. These recommendations are not validated scientifically. The microplate method for minimum inhibitory concentration (MIC) determination was used to compare wild with cultivated, and fresh with dry plant materials. The MIC values obtained ranged from 1.25 to 0.01 mg/ml. MIC values ≤0.3 mg/ml were considered as cut off point between effective and none effective inhibition. The multilevel linear models (hierarchical linear models), both unadjusted and adjusted models were employed. The plant (name) was considered as level-2 or higher level, while the actual observation was level-1 or lower level. The crude estimates of the odds ratio indicated that wild is significantly 0.57 times less likely than garden to yield MIC values of more than 0.3 mg/ml (p-value = 0.005). Also, fresh are about 4.195 times more likely than dry to yield MIC scores of more than 0.3 mg/ml (p-value < 0.001). Adjusting for conditions “dry and fresh”, microbe and solvent; wild is significantly 0.52 times less likely than garden to yield MIC values of more than 0.3 mg/ml (p-value = 0.003). On the other hand, when adjusting for “wild or garden”, type of solvents and type of microbes; fresh is significantly 4.202 times more likely than dry to yield MIC values of more than 0.3 mg/ml (p-value < 0.001). These results partially support farmers claiming that wild plant materials are more potent than the grown ones. On the contrary, the results are in favour of drying plant materials.
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Gutu, Ketema Tolossa. "Bioassay-guided phytochemical study of indigenous medicinal plants of Ethiopia." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/28964.

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In many developing countries, farmers and pastoralists still rely on their indigenous knowledge, practices and locally available plants to control nematode parasitic infections, both in livestock and humans. The overall aim of my thesis was to undertake bioassay-guided phyto-chemical study of extracts and their constituents from Ethiopian anti-parasitic plants used by healers to control gastrointestinal nematode parasites in livestock to validate their ethno-medicinal use and to characterise and identify their active ingredients. As a first experiment (Chapter Three), four types of crude extracts (water, 70% methyl-alcohol, absolute methanol and acetone) of four indigenous Ethiopian medicinal plants (Adenia species, Cissus ruspolii, Ipomoea eriocarpa and Euphorbia thymifolia) were screened against Teladorsagia circumcincta egg hatching in vitro, not only as a first step to validate the traditional healers claim but also to choose the most promising plant extract(s) for further phyto-chemical studies. The egg hatching inhibition (EHI) test results revealed that the anti-parasitic properties of these plants depended on plant species, dose, and solvent polarity. The water extracts of both C. ruspolii and Adenia sp. exhibited largest, up to 100% EHI but also larval migration inhibition activities, and were selected for further studies. The second experiment (Chapter Four) assessed the nature of active constituents in these extracts by physico-chemical methods. It was observed that the major constituents of both plant extracts responsible for the EHI activities are likely highly polar, water-soluble, small and moderately heat-labile molecules. The third and fourth experiments (Chapters Five and Six) consisted of separating Cissus ruspolii and Adenia sp. water extracts into discrete fractions by gel-permeation chromatography, EHI tests of Bio-Gel P-2 fractions followed by thin layer chromatography (TLC) profiling of these fractions to detect separated spots (in day light, under UV-light or after staining with various staining reagents) and also to see how elution patterns of separated spots affected by column parameters. The EHI tests on the fractions obtained revealed that the active constituents of C. ruspolii and Adenia sp. water crude extracts were eluted into few fractions based on their molecular sizes. The TLC profilings of these fractions identified spot patterns of active and inactive fractions, which allowed pooling of active constituents based on their EHI and TLC profiling into three pools for each plant. The fifth experiment (Chapter Seven) was to isolate and purify compounds from these pools using various preparative planar and column chromatographic methods. Sequential applications of column chromatography followed by preparative thin layer chromatography isolated and purified five active compounds from C. ruspolii and two active compounds from Adenia sp. The sixth experiment (Chapter Eight) was to characterize and propose/elucidate structures of compounds from the active fractions using chromatographic, analytical and spectroscopic methods. In this regard, the structures of two oleanane type triterpenoid saponins isolated from one of active fractions of Adenia sp. were proposed based on their mass spectrometry (MS) and nuclear magnetic resonance (NMR) data with support of compounds property, TLC and literature. Similar outcomes for C. ruspolii were not achieved due to lack of sufficient sample to run 13C-nuclear magnetic resonance spectroscopy and distortionless enhancement by polarization transfer (DEPT), contamination of some purified compounds with ill-characterised substance from the preparative TLC matrix and in some cases mass spectrometry (MS) and nuclear magnetic resonance (NMR) data did not support each other. The last experiment (Chapter Nine) was to assess anthelmintic efficacy and safety of C. ruspolii and Adenia sp. crude water extracts in Heligmosomoides bakeri infected mice. This in vivo test revealed that both plant extracts exhibited significant reduction in worm burdens and worm egg excretion, with moderate effects on haematology and organ weights at tolerated dosages. In conclusion, both in vitro and in vivo data revealed that Adenia sp. and C. ruspolii have anthelmintic properties, thus validating traditional healer claims and supporting ethno-medicinal use. The bioassay-guided phytochemical study resulted in the isolation of a number of active compounds from these plants, for some of which a structure has been proposed.
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Boukes, Gerhardt Johannes. "The in vitro biological activities of three Hypoxis species and their active compounds." Thesis, Nelson Mandela Metropolitan University, 2010. http://hdl.handle.net/10948/1228.

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The African potato is used as an African traditional medicine for its nutritional and medicinal properties. Most research has been carried out on H. hemerocallidea, with very little or nothing on other Hypoxis spp. The main aim of this project was to provide scientific data on the anticancer, anti-inflammatory and antioxidant properties of H. hemerocallidea, H. stellipilis and H. sobolifera chloroform extracts and their active compounds. The hypoxoside and phytosterol contents of the three Hypoxis spp. were determined using TLC, HPLC and GC. H. hemerocallidea and H. sobolifera chloroform extracts contained the highest amounts of hypoxoside and β-sitosterol, respectively. For the anticancer properties, cytotoxicity of the Hypoxis extracts and its purified compounds were determined against the HeLa, HT-29 and MCF-7 cancer cell lines (using MTT), and PBMCs (using CellTiter-Blue®). H. sobolifera had the best cytotoxicity against the three cancer cell lines, whereas H. stellipilis stimulated HeLa and HT-29 cancer cell growth. IC50 values of hypoxoside and rooperol were determined. DNA cell cycle arrest (using PI staining) occurred in the late G1/early S (confirmed by increased p21Waf1/Cip1 expression) and G2/M phases after 15 and 48 hrs, respectively, when treated with Hypoxis extracts and rooperol. H. sobolifera and rooperol activated caspase-3 and -7 (using fluorescently labelled antibodies) in HeLa and HT-29 cancer cells, and caspase-7 in MCF-7 cancer cells after 48 hrs. Annexin V binding to phosphatidylserines in rooperol treated U937 cells confirmed early apoptosis after 15 hrs. The TUNEL assay showed DNA fragmentation in the three cancer cell lines when treated with H. sobolifera and rooperol for 48 hrs. A shift pass the G2/M phase has led to the investigation of endoreduplication, which was confirmed by cell/nucleus size, and anti-apoptotic proteins (Akt, phospho-Akt, phospho-Bcl-2 and p21Waf1/Cip1). U937 cell differentiation to monocyte-macrophages was optimized using PMA and 1,25(OH)2D3, which was confirmed by morphological and biochemical changes. For the anti-inflammatory properties, Hypoxis extracts and rooperol significantly increased NO production in monocyte-macrophages (pre-loaded with DAF-2 DA) and phagocytosis of pHrodoTM E. coli BioParticles®. The treatments had no effect on COX-2 expression in monocyte-macrophages. The phytosterols significantly increased IL-1β and IL-6 secretion xv (using the FlowCytomix Multiplex human Th1/Th2 10plex Kit I) in the PBMCs of one donor. For the antioxidant properties, Hypoxis extracts and rooperol significantly increased ROS production in undifferentiated and differentiated U937 cells, which were pre-loaded with DCFH-DA. Hypoxis extracts and purified compounds had ferric reducing activities, but only rooperol had ferric reducing activities significantly greater than ascorbic acid. β-sitosterol, campesterol and cholesterol significantly increased SOD activity in Chang liver cells, while H. stellipilis, H. sobolifera and rooperol decreased SOD activity. Anticancer, anti-inflammatory and antioxidant properties of the Hypoxis extracts may be attributed to the β-sitosterol content, because Hypoxis chloroform extracts contained very little or no hypoxoside. Unidentified compounds, and synergistic and additive effects of the compounds may have contributed to the biological effects. This study confirms previous reports that rooperol is the active compound. Results provide scientific data on the medicinal properties of one of the most frequently used medicinal plants in South Africa.
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Mulubwe, Ngosa. "A comparative study of the Phosphodiesterase 4 inhibitory activity of Artemisia Afra, Leonotis Leonorus and mentha longifolia plant medicines. /." Thesis, Online Access, 2007. http://etd.uwc.ac.za/usrfiles/modules/etd/docs/etd_gen8Srv25Nme4_7163_1257926263.pdf.

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Books on the topic "Medicinal plants (In vitro study)"

1

India, Export-Import Bank of. Indian medicinal plants: A sector study. Mumbai: Export-Import Bank of India, 1997.

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Protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants. New York: Humana Press, 2009.

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Jain, S. Mohan, and Praveen K. Saxena, eds. Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-287-2.

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India, Export-Import Bank of. Pharmaceuticals & herbal medicines: A sector study. [Mumbai]: Export-Import Bank of India, 1997.

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Cousins, D. J. Medicinal plants - metabolite production in vitro 1973-1993: A bibliography compiled from the CAB ABSTRACTS database. Oxon: CAB International, 1994.

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Farooq, S. 555 medicinal plants: Field and laboratory manual, identification with its phytochemical and in vitro studies data. Dehradun: International Book Distributors, 2005.

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Jain, S. Mohan, ed. Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3332-7.

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Gertsson, Ulla E. Development of a micropropagation method for Senecio x hybridus Hyl. and study of the progeny plants. Alnarp: Swedish University of Agricultural Sciences, Dept. of Horticultural Science, 1987.

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Jinju, Muhammadu Hambali. African traditional medicine: A case study of Hausa medicinal plants and therapy. Zaria, Nigeria: M.H. Jinju, 1990.

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Saltzman, Mark. Chinese herbal medicine: Materia medica study guide. Brookline, Mass: Complementary Medicine Press, 2000.

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Book chapters on the topic "Medicinal plants (In vitro study)"

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Verma, Peeyushi, and Rakhi Chaturvedi. "In Vitro Plant Cell Cultures: A Route to Production of Natural Molecules and Systematic In Vitro Assays for their Biological Properties." In Medicinal Plants, 215–41. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-31269-5_10.

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Verma, Virendra M. "Black Pepper: Health Benefits, In Vitro Multiplication, and Commercial Cultivation." In Medicinal Plants, 111–27. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-31269-5_5.

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Sri Devi, Masilamani, Krishnamoorthy Vinothini, Blassan P. George, Sudharshan Sekar, Heidi Abrahamse, Bettine van Vuuren, and Arjun Pandian. "In Vitro Calli Induction, Biomass Accumulation and Different Biological Activity of Leucas aspera (willd.) Linn." In Medicinal Plants, 297–316. Boca Raton : Taylor & Francis, 2018. | “A CRC title, part of the Taylor & Francis imprint, a member of the Taylor & Francis Group, the academic division of T&F Informa plc.”: CRC Press, 2018. http://dx.doi.org/10.1201/9781351046510-16.

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Vinothini, Krishnamoorthy, Masilamani Sri Devi, Sudharshan Sekar, Blassan P. George, Heidi Abrahamse, Bettine van Vuuren, and Arjun Pandian. "In Vitro Plant Regeneration, Comparative Biochemical and Antioxidant Potential of Calli and Seeds of Sesbania grandiflora (L.) Poiret." In Medicinal Plants, 355–78. Boca Raton : Taylor & Francis, 2018. | “A CRC title, part of the Taylor & Francis imprint, a member of the Taylor & Francis Group, the academic division of T&F Informa plc.”: CRC Press, 2018. http://dx.doi.org/10.1201/9781351046510-19.

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Arunachalam, Karuppusamy, Puthanpura Sasidharan Sreeja, Domingos Tabajara de Oliveira Martins, and Parimelazhagan Thangaraj. "Antidiabetic Activity by the In Vitro α-Amylase and α-Glucosidase Inhibitory Action of Indian Ayurvedic Medicinal Plants." In Medicinal Plants, 101–10. Boca Raton : Taylor & Francis, 2018. | “A CRC title, part of the Taylor & Francis imprint, a member of the Taylor & Francis Group, the academic division of T&F Informa plc.”: CRC Press, 2018. http://dx.doi.org/10.1201/9781351046510-6.

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Srivastava, S., and A. K. Srivastava. "In Vitro Azadirachtin Production." In Bioactive Molecules and Medicinal Plants, 233–54. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-74603-4_12.

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Tripathi, Prachi, Lubana Shahin, Ankush Sangra, Richa Bajaj, Alok Arun, and Juan A. Negron Berrios. "Current Status and Future Prospects for Select Underutilized Medicinally Valuable Plants of Puerto Rico: A Case Study." In Medicinal Plants, 81–110. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-31269-5_4.

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Kukułczanka, K., and J. Budzianowski. "Dionaea muscipula Ellis (Venus Flytrap): In Vitro Cultures and in Vitro Production of Secondary Metabolites." In Medicinal and Aromatic Plants XII, 50–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-662-08616-2_4.

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Kato, M. "Camellia sinensis L. (Tea): In Vitro Regeneration." In Medicinal and Aromatic Plants II, 82–98. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73617-9_5.

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Williams, P. D. "Capsicum spp. (Peppers): In Vitro Production of Capsaicin." In Medicinal and Aromatic Plants IV, 51–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77004-3_4.

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Conference papers on the topic "Medicinal plants (In vitro study)"

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Khair, Nedaa Kamalalden. "Activity of Antibiotic Producing Bacteria Isolated from Rhizosphere Soil Region of Different Medicinal Plants." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0093.

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The rhizosphere soil of medicinal plants is rich in microorganisms that develop antibiotics as natural mechanism of protection against other microbes that live in their vicinity. The present study aims to explore the production of antibacterial agents from rhizosphere soil bacteria of 11 medicinal plants and determine their activity against Gram-negative (Pseudomonas aeruginosa, Escherichia coli) and Gram-positive (Bacillus cereus, Staphylococcus aureus) bacteria. Soil samples were collected and used to isolate antibiotic producing bacteria (APB). Those isolates (108) were first tested using Cross-streak method against test bacteria. Then, isolates that showed a positive antibacterial effect (12) were tested by antibiotic susceptibility test (AST) of their cell free supernatant (CFS) and their extracellular and intracellular secondary metabolites extraction which gave positive results. Staphylococcus aureus found to be the most sensitive test bacteria with inhibitory zones ranging from 13.5 - 19 mm. Moreover, combinatorial effect of isolates CFS with two organic acids (3% Acetic acid and 0.4 mg/ml Acetylsalicylic acid), two commercial antibiotics (0.016 mg/ml Augmentin and 0.128 mg/ml Doxycycline), and two pure antibiotics (10 mcg/disk Penicillin and 25mcg/disk Carbenicillin) was in vitro evaluated using AST. The combinations of CFS-carbenicillin showed a marked synergistic activity against all test bacteria. The presence of possible antibacterial agents as acetic acid, lactic acid and citric acid in CFS of APB was confirmed by HPLC analysis. Ultimately, in vitro antibacterial study for rhizosphere soil bacteria in this work suggests the possibility of using these bacterial metabolites in clinical infections caused by selected test bacteria, especially when they combine with antibiotics or organic acids.
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Erland, LAE, and PK Saxena. "A New Approach to Optimizing Propagation and Study of Medicinal Plants In Vitro: Profiling of Endogenous Growth Regulators and Human Neurotransmitters by LC-MS." In Abstracts of the NHPRS – The 15th Annual Meeting of the Natural Health Products Research Society of Canada (NHPRS). Georg Thieme Verlag KG, 2018. http://dx.doi.org/10.1055/s-0038-1644963.

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Saowakon, N., Y. Camchuen, N. Changklungmoa, P. Kueakhai, and P. Sobhon. "In vitro study of the Plumbago indica root extract on Fasciola gigantica." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399742.

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Hilmi, Yasmin, and Muna Abushama. "In-vitro Cytotoxicity Of Selected Sudanese Medicinal Plants With Potential Antitumor Activity." In Qatar Foundation Annual Research Conference Proceedings. Hamad bin Khalifa University Press (HBKU Press), 2014. http://dx.doi.org/10.5339/qfarc.2014.hbpp0213.

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Cutcovschi-Mustuc, A., N. Ciorchina, and M. Trofim. "CULTIVATION METHOD IN VITRO OF MEDICINAL PLANT WITHANIA SOMNIFERA L. (DUNAL)." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1299-1302.

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Mahmoud, A., P. Mäser, M. Kaiser, M. Hamburger, and S. Khalid. "Screening of Selected Sudanese Medicinal Plants for In vitro Activity Against Protozoal Neglected Tropical Diseases." In GA 2017 – Book of Abstracts. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1608461.

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Shanker, Dr Karuna, and Pooja Gaur. "In vitro Screening of alcoholic and hydroalcoholic extracts of Ayurvedic medicinal plants for the management of hyperlipidemia." In 5th International Electronic Conference on Medicinal Chemistry. Basel, Switzerland: MDPI, 2019. http://dx.doi.org/10.3390/ecmc2019-06409.

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Gao, Liwen, and Xiaohua Lin. "A study on the automatic recognition system of medicinal plants." In 2012 2nd International Conference on Consumer Electronics, Communications and Networks (CECNet). IEEE, 2012. http://dx.doi.org/10.1109/cecnet.2012.6201425.

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Wang, Xiuying. "Study on Continuous Cropping Obstacle and Control Strategy of Medicinal Plants." In 2017 3rd International Conference on Economics, Social Science, Arts, Education and Management Engineering (ESSAEME 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/essaeme-17.2017.172.

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Khamidullina, L. A., P. D. Tobysheva, E. A. Rybina, O. E. Cherepanova, and A. V. Pestov. "Plant growth biostimulants based on synthetic polyaminosaccharides." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.117.

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