Relevant bibliographies by topics / Mediterranean fruit-fly Sex determination, Genetic

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Mediterranean fruit-fly Sex determination, Genetic'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "Mediterranean fruit-fly Sex determination, Genetic"

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Basso, Alicia, and Ariane Sonvico. "Identification and in situ hybridization to mitotic chromosomes of a molecular marker linked to maleness in Anastrepha fraterculus (Wied.)." Journal of Applied Biotechnology & Bioengineering 7, no. 6 (December 7, 2020): 237–40. http://dx.doi.org/10.15406/jabb.2020.07.00239.

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The present report shows the molecular identification, isolation and citologically localization of a DNA-sequence from the South American fruit fly Anastrepha fraterculus (DIPTERA: Tephritidae) involved in sex- determination. It belongs to the Tephritidae family, the true fruit flies which are consider a pest of fruit crops. The sex determination system is of vital importance in the genetic control of the fruit fly pest: Sterile Insect Technique which unlike chemical control tactics, is environmentally friendly and does not pose any health concerns. We used in situ hybridization on mitotic chromosomes for localizing the primary sex determination factor in this fruit fly pest. Our results show that in Anastrepha fraterculus the Y chromosome is responsible for sex determination
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Franz, G., E. Gencheva, and Ph Kerremans. "Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata." Genome 37, no. 1 (February 1, 1994): 72–82. http://dx.doi.org/10.1139/g94-009.

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In the existing genetic sexing strains for the medfly, Ceratitis capitata, male recombination leads to breakdown of the sexing mechanism under mass rearing conditions. The rate of breakdown depends on the recombination frequency and on the fitness of the recombinants. We have tested two different sexing genes, white pupa and a temperature sensitive lethal, in combination with the translocation T(Y;5)30C. Both sexing strains broke down, although at very different rates. In the case of the white pupa strain, 3.5% recombinants were observed after rearing the strain for 15 generations. The second strain, utilizing white pupa and the temperature sensitive lethal as selectable markers, already reached a comparable level after six generations and was broken down completely in the ninth generation. In these strains the frequency of recombination is high because the breakpoint of T(Y;5)30C and the sexing gene(s) are far apart. To remedy the situation, we have isolated four new translocations with breakpoints located closer to the sexing genes. Mass rearing was simulated for several generations with strains based on these translocations and no breakdown was observed under the conditions used.Key words: medfly, sterile insect technique, genetic sexing, recombination.
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Kerremans, Ph, E. Gencheva, and G. Franz. "Genetic and cytogenetic analysis of Y-autosome translocations in the Mediterranean fruit fly, Ceratitis capitata." Genome 35, no. 2 (April 1, 1992): 264–72. http://dx.doi.org/10.1139/g92-041.

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Radiation-induced translocations in the Mediterranean fruit fly, Ceratitis capitata, linking the Y chromosome to either autosome 3 or 4 produced pseudolinkage between sex and the mutations dark pupa (dp) and apricot eye (ap), respectively. The genetic behaviour of six new strains is described and the structural basis of five of them is determined through analysis of polytene and mitotic chromosomes. Five strains exhibited low levels of recombination; however, one strain produced a larger number than expected of aberrant, wild-type females. We provide evidence that this is the consequence of the survival of adjacent-1 segregation products until adulthood.Key words: medfly, mass rearing, genetic sexing, recombination, segregation.
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Meccariello, Angela, Marco Salvemini, Pasquale Primo, Brantley Hall, Panagiota Koskinioti, Martina Dalíková, Andrea Gravina, et al. "Maleness-on-the-Y (MoY) orchestrates male sex determination in major agricultural fruit fly pests." Science 365, no. 6460 (August 29, 2019): 1457–60. http://dx.doi.org/10.1126/science.aax1318.

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In insects, rapidly evolving primary sex-determining signals are transduced by a conserved regulatory module controlling sexual differentiation. In the agricultural pest Ceratitis capitata (Mediterranean fruit fly, or Medfly), we identified a Y-linked gene, Maleness-on-the-Y (MoY), encoding a small protein that is necessary and sufficient for male development. Silencing or disruption of MoY in XY embryos causes feminization, whereas overexpression of MoY in XX embryos induces masculinization. Crosses between transformed XY females and XX males give rise to males and females, indicating that a Y chromosome can be transmitted by XY females. MoY is Y-linked and functionally conserved in other species of the Tephritidae family, highlighting its potential to serve as a tool for developing more effective control strategies against these major agricultural insect pests.
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Cladera, Jorge L., and M. Alejandra Delprat. "Genetic and cytological mapping of a "Y–2" translocation in the Mediterranean fruit fly Ceratitis capitata." Genome 38, no. 6 (December 1, 1995): 1091–97. http://dx.doi.org/10.1139/g95-145.

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In this paper we analyze genetically and cytologically a Y – chromosome 2 translocation with several markers, some of which are potentially useful for large scale sex separation. The breakpoint of this Y–2 translocation is located at region 6B on the trichogene polytene chromosome map. It was found that, in strains carrying this TY–2, only 40% of the fertilized eggs survived to the adult stage, 26% of them dying as embryos, 27% as larvae, and 7% as pupae. Early lethality is explained by the nonviability of adjacent-1 products of meiosis containing a deletion of section 1A–6B. The reciprocal segregation products, carrying this chromosome segment in triplicate, survive until late stages. By analyzing the phenotype of these individuals we conclude that all markers used in this study are located outside the triplicated region and that the male determining factor is not included in the piece of the Y chromosome translocated to chromosome 2. The male recombination frequencies of several genes located on chromosome 2 relative to the breakpoint of translocation T5038 have also been studied here.Key words: genetic sexing strain, Medfly polytene chromosome, adjacent-1 product, translocation breakpoint mapping.
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Wrischnik, Lisa A., John R. Timmer, Lisa A. Megna, and Thomas W. Cline. "Recruitment of the Proneural Gene scute to the Drosophila Sex-Determination Pathway." Genetics 165, no. 4 (December 1, 2003): 2007–27. http://dx.doi.org/10.1093/genetics/165.4.2007.

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Abstract In flies, scute (sc) works with its paralogs in the achaete-scute-complex (ASC) to direct neuronal development. However, in the family Drosophilidae, sc also acquired a role in the primary event of sex determination, X chromosome counting, by becoming an X chromosome signal element (XSE)—an evolutionary step shown here to have occurred after sc diverged from its closest paralog, achaete (ac). Two temperature-sensitive alleles, scsisB2 and scsisB3, which disrupt only sex determination, were recovered in a powerful F1 genetic selection and used to investigate how sc was recruited to the sex-determination pathway. scsisB2 revealed 3′ nontranscribed regulatory sequences likely to be involved. The scsisB2 lesion abolished XSE activity when combined with mutations engineered in a sequence upstream of all XSEs. In contrast, changes in Sc protein sequence seem not to have been important for recruitment. The observation that the other new allele, scsisB3, eliminates the C-terminal half of Sc without affecting neurogenesis and that scsisB1, the most XSE-specific allele previously available, is a nonsense mutant, would seem to suggest the opposite, but we show that housefly Sc can substitute for fruit fly Sc in sex determination, despite lacking Drosophilidae-specific conserved residues in its C-terminal half. Lack of synergistic lethality among mutations in sc, twist, and dorsal argue against a proposed role for sc in mesoderm formation that had seemed potentially relevant to sex-pathway recruitment. The screen that yielded new sc alleles also generated autosomal duplications that argue against the textbook view that fruit fly sex signal evolution recruited a set of autosomal signal elements comparable to the XSEs.
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Nguyen, Thu N. M., Amanda Choo, and Simon W. Baxter. "Lessons from Drosophila: Engineering Genetic Sexing Strains with Temperature-Sensitive Lethality for Sterile Insect Technique Applications." Insects 12, no. 3 (March 13, 2021): 243. http://dx.doi.org/10.3390/insects12030243.

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A major obstacle of sterile insect technique (SIT) programs is the availability of robust sex-separation systems for conditional removal of females. Sterilized male-only releases improve SIT efficiency and cost-effectiveness for agricultural pests, whereas it is critical to remove female disease-vector pests prior to release as they maintain the capacity to transmit disease. Some of the most successful Genetic Sexing Strains (GSS) reared and released for SIT control were developed for Mediterranean fruit fly (Medfly), Ceratitis capitata, and carry a temperature sensitive lethal (tsl) mutation that eliminates female but not male embryos when heat treated. The Medfly tsl mutation was generated by random mutagenesis and the genetic mechanism causing this valuable heat sensitive phenotype remains unknown. Conditional temperature sensitive lethal mutations have also been developed using random mutagenesis in the insect model, Drosophila melanogaster, and were used for some of the founding genetic research published in the fields of neuro- and developmental biology. Here we review mutations in select D. melanogaster genes shibire, Notch, RNA polymerase II 215kDa, pale, transformer-2, Dsor1 and CK2α that cause temperature sensitive phenotypes. Precise introduction of orthologous point mutations in pest insect species with CRISPR/Cas9 genome editing technology holds potential to establish GSSs with embryonic lethality to improve and advance SIT pest control.
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KaramiNejadRanjbar, Mohammad, Kolja N. Eckermann, Hassan M. M. Ahmed, Héctor M. Sánchez C., Stefan Dippel, John M. Marshall, and Ernst A. Wimmer. "Consequences of resistance evolution in a Cas9-based sex conversion-suppression gene drive for insect pest management." Proceedings of the National Academy of Sciences 115, no. 24 (May 29, 2018): 6189–94. http://dx.doi.org/10.1073/pnas.1713825115.

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The use of a site-specific homing-based gene drive for insect pest control has long been discussed, but the easy design of such systems has become possible only with the recent establishment of CRISPR/Cas9 technology. In this respect, novel targets for insect pest management are provided by new discoveries regarding sex determination. Here, we present a model for a suppression gene drive designed to cause an all-male population collapse in an agricultural pest insect. To evaluate the molecular details of such a sex conversion-based suppression gene drive experimentally, we implemented this strategy in Drosophila melanogaster to serve as a safe model organism. We generated a Cas9-based homing gene-drive element targeting the transformer gene and showed its high efficiency for sex conversion from females to males. However, nonhomologous end joining increased the rate of mutagenesis at the target site, which resulted in the emergence of drive-resistant alleles and therefore curbed the gene drive. This confirms previous studies that simple homing CRISPR/Cas9 gene-drive designs will be ineffective. Nevertheless, by performing population dynamics simulations using the parameters we obtained in D. melanogaster and by adjusting the model for the agricultural pest Ceratitis capitata, we were able to identify adequate modifications that could be successfully applied for the management of wild Mediterranean fruit fly populations using our proposed sex conversion-based suppression gene-drive strategy.
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Primo, Pasquale, Angela Meccariello, Maria Grazia Inghilterra, Andrea Gravina, Giuseppe Del Corsano, Gennaro Volpe, Germano Sollazzo, et al. "Targeting the autosomal Ceratitis capitata transformer gene using Cas9 or dCas9 to masculinize XX individuals without inducing mutations." BMC Genetics 21, S2 (December 2020). http://dx.doi.org/10.1186/s12863-020-00941-4.

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Abstract Background Females of the Mediterranean fruit fly Ceratitis capitata (Medfly) are major agricultural pests, as they lay eggs into the fruit crops of hundreds of plant species. In Medfly, female sex determination is based on the activation of Cctransformer (Cctra). A maternal contribution of Cctra is required to activate Cctra itself in the XX embryos and to start and epigenetically maintain a Cctra positive feedback loop, by female-specific alternative splicing, leading to female development. In XY embryos, the male determining Maleness-on-the-Y gene (MoY) blocks this activation and Cctra produces male-specific transcripts encoding truncated CcTRA isoforms and male differentiation occurs. Results With the aim of inducing frameshift mutations in the first coding exon to disrupt both female-specific and shorter male-specific CcTRA open reading frames (ORF), we injected Cas9 ribonucleoproteins (Cas9 and single guide RNA, sgRNA) in embryos. As this approach leads to mostly monoallelic mutations, masculinization was expected only in G1 XX individuals carrying biallelic mutations, following crosses of G0 injected individuals. Surprisingly, these injections into XX-only embryos led to G0 adults that included not only XX females but also 50% of reverted fertile XX males. The G0 XX males expressed male-specific Cctra transcripts, suggesting full masculinization. Interestingly, out of six G0 XX males, four displayed the Cctra wild type sequence. This finding suggests that masculinization by Cas9-sgRNA injections was independent from its mutagenic activity. In line with this observation, embryonic targeting of Cctra in XX embryos by a dead Cas9 (enzymatically inactive, dCas9) also favoured a male-specific splicing of Cctra, in both embryos and adults. Conclusions Our data suggest that the establishment of Cctra female-specific autoregulation during the early embryogenesis has been repressed in XX embryos by the transient binding of the Cas9-sgRNA on the first exon of the Cctra gene. This hypothesis is supported by the observation that the shift of Cctra splicing from female to male mode is induced also by dCas9. Collectively, the present findings corroborate the idea that a transient embryonic inactivation of Cctra is sufficient for male sex determination.
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Carraretto, Davide, Nidchaya Aketarawong, Alessandro Di Cosimo, Mosè Manni, Francesca Scolari, Federica Valerio, Anna R. Malacrida, Ludvik M. Gomulski, and Giuliano Gasperi. "Transcribed sex-specific markers on the Y chromosome of the oriental fruit fly, Bactrocera dorsalis." BMC Genetics 21, S2 (December 2020). http://dx.doi.org/10.1186/s12863-020-00938-z.

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Abstract Background The Oriental fruit fly, Bactrocera dorsalis, is a highly polyphagous invasive species with a high reproductive potential. In many tropical and subtropical parts of the world it ranks as one of the major pests of fruits and vegetables. Due to its economic importance, genetic, cytogenetic, genomic and biotechnological approaches have been applied to understand its biology and to implement the Sterile Insect Technique, currently a part of area-wide control programmes against this fly. Its chromosome complement includes five pairs of autosomes and the sex chromosomes. The X and Y sex chromosomes are heteromorphic and the highly heterochromatic and degenerate Y harbours the male factor BdMoY. The characterization of the Y chromosome in this fly apart from elucidating its role as primary sex determination system, it is also of crucial importance to understand its role in male biology. The repetitive nature of the Y chromosome makes it challenging to sequence and characterise. Results Using Representational Difference Analysis, fluorescent in situ hybridisation on mitotic chromosomes and in silico genome resources, we show that the B. dorsalis Y chromosome harbours transcribed sequences of gyf, (typo-gyf) a homologue of the Drosophila melanogaster Gigyf gene, and of a non-LTR retrotransposon R1. Similar sequences are also transcribed on the X chromosome. Paralogues of the Gigyf gene are also present on the Y and X chromosomes of the related species B. tryoni. Another identified Y-specific repetitive sequence linked to BdMoY appears to be specific to B. dorsalis. Conclusions Our random scan of the Y chromosome provides a broad picture of its general composition and represents a starting point for further applicative and evolutionary studies. The identified repetitive sequences can provide a useful Y-marking system for molecular karyotyping of single embryos. Having a robust diagnostic marker associated with BdMoY will facilitate studies on how BdMoY regulates the male sex determination cascade during the embryonic sex-determination window. The Y chromosome, despite its high degeneracy and heterochromatic nature, harbours transcribed sequences of typo-gyf that may maintain their important function in post-transcriptional mRNA regulation. That transcribed paralogous copies of Gigyf are present also on the X and that this genomic distribution is maintained also in B. tryoni raises questions on the evolution of sex chromosomes in Bactrocera and other tephritids.
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Dissertations / Theses on the topic "Mediterranean fruit-fly Sex determination, Genetic"

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Untalan, Pia Marie. "The use of suppression subtractive hybridization in the identification of a novel gene encoding a protein containing a BTB-POZ domain in the Mediterranean fruit fly, Ceratitis capitata." Thesis, University of Hawaii at Manoa, 2002. http://proquest.umi.com/pqdweb?index=0&did=765064591&SrchMode=1&sid=2&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1208568664&clientId=23440.

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Book chapters on the topic "Mediterranean fruit-fly Sex determination, Genetic"

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Franz, Gerald, and Philippe Kerremans. "Requirements and Strategies for the Development of Genetic Sex Separation Systems with Special Reference to the Mediterranean Fruit Fly Ceratitis Capitata." In Fruit Flies and the Sterile Insect Technique, 113–22. CRC Press, 2019. http://dx.doi.org/10.1201/9781351072168-9.

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Conference papers on the topic "Mediterranean fruit-fly Sex determination, Genetic"

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Saccone, Giuseppe. "Genetics of sex determination in the Mediterranean fruit fly: From basic to applied research." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.92765.

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