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Journal articles on the topic 'MEGA7'

Author: Grafiati

Published: 3 June 2025

Last updated: 20 July 2025

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1

Kumar, Sudhir, Glen Stecher, and Koichiro Tamura. "MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets." Molecular Biology and Evolution 33, no. 7 (2016): 1870–74. http://dx.doi.org/10.1093/molbev/msw054.

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Wu, Shanshan, Jeffrey Haltom, Wenyi Zhao, Jingwen Yang, Zhan Zhou, and Xun Gu. "Evolution and functional divergence of the ERBB receptor family." Pharmacogenomics 22, no. 8 (2021): 473–84. http://dx.doi.org/10.2217/pgs-2020-0158.

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Aim: The ERBB gene family plays an important role in cell proliferation and differentiation, and aberrant activations could result in tumorigenesis, which makes this gene family an attractive drug target in the area of precision oncology. Materials & methods: Functional divergence analysis and conservation analysis were performed using ClustalW, MEGA7 and DIVERGE3 software. Results: One hundred and forty five functional divergence residues sites, 94 totally conserved sites and averagely 345 conserved sites of individual gene member were obtained. Some have been reported to play role in dru

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Makvandi, Manoochehr, Gholam Abbas Kaydani, Heshmatollah Shahbazian, Alireza Teimoori, Fakher Rahim, and Sajjad Aslani. "The complete genome sequence BK polyomavirus study in kidney transplanted patients." Journal of Nephropathology 8, no. 2 (2018): 15. http://dx.doi.org/10.15171/jnp.2019.15.

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Background: BK polyomavirus is a member of the Polyomaviridae. This virus has spread worldwide and up to 82% of the world populations are serologically positive. BK polyomavirus usually transmits through inhalation or fecal-oral way in childhood, as well as is likely to cause an asymptomatic disease. The virus can be reactivated in people who are immunocompromised. Objectives: In this study we aimed to determine the complete genome sequence of BK polyomavirus in 5 patients receiving kidney transplantation in Golestan hospital, Ahvaz, south west of Iran. Materials and Methods: The study was per

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Ніколаєнко, О. А. "ЛЕКСИКО-СЕМАНТИЧНИЙ АНАЛІЗ ЛАТИНСЬКОМОВНИХ ОДНОСЛІВНИХ КЛІНІЧНИХ ТЕРМІНІВ З ТЕРМІНОЕЛЕМЕНТАМИ MACR-, MEGA- (MEGAL-)". Nova fìlologìâ, № 86 (15 грудня 2022): 132–36. http://dx.doi.org/10.26661/2414-1135-2022-86-19.

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У статті проведено лексико-семантичний аналіз терміноелементів macr- та mega- (megal-) у однослівних клінічних термінах, з’ясовано, що зазначені терміноелементи походять від давньогрецьких прикметників і перекладаються як «великий, високий, глибокий, сильний, значимий». Однак в однослівних клінічних термінах досліджувані терміноелементи можуть проявляти різні лексико-семантичні відтінки. Для компоненту macr- характерне значення, що вказує на патологічне збільшення розміру, діаметра, довжини, ширини, підвищення гучності, множинність тощо. Терміноелемент mega- (megal-) також вказує на патологічн

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Fitria, Murni, Anggi Saputra, Rahmat Hidayat, and Niken Feladita. "Analisis Protein Gen LCYB pada Mikroalga Laut Phaeodactylum tricornutum menggunakan Pendekatan Bioinformatika." IJCA (Indonesian Journal of Chemical Analysis) 5, no. 2 (2022): 138–44. http://dx.doi.org/10.20885/ijca.vol5.iss2.art8.

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LCYB merupakan gen yang memproduksi enzim Lycopene Beta Cyclase. Enzim Lycopene Beta Cyclase beperan dalam mengubah likopen menjadi ß-karoten. Gen LCYB terdapat pada diatom maupun mikroalga hijau. Berbeda dengan mikroalga hijau, penelitian mengenai struktur protein Lycopene Beta Cyclase selaku produk gen LCYB pada diatom masih minim publikasi. Dalam penelitian ini dilakukan prediksi struktur produk gen LCYB pada diatom P. tricornutum secara bioinformatika. Untuk menganalisis perbedaan produk gen LCYB pada diatom dan mikroalga hijau, produk gen LCYB pada diatom P. tricornutum dibandingkan denga

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Lantang, Daniel, and Arsyam Mawardi. "Molecular Diversity of Bacillus thuringiensis and Bioinformatics Analysis of Local Isolate of Auky Island, Padaido District in Biak Numfor Papua as a Control of Anopheles Mosquito Larvae." Journal of Pure and Applied Microbiology 15, no. 4 (2021): 2295–301. http://dx.doi.org/10.22207/jpam.15.4.52.

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This research aims to analyze the level of similarity and diversity among local isolates of B. thuringiensis Auky Island Padaido District in Biak Numfor Regency with NCBI gene bank base, the basis of which is to obtain B. thuringiensis isolates from jayapura local isolates that can act as controllers of Anopheles mosquito larvae. Several steps in the research are 16s gene amplification, PCR product purification, cloning using pTA2 vectors and transformation into competent E. coli Zymo 5α cells, confirmation with PCR colonies, recombinant plasmid isolation, sequencing analysis and phylogenetic

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Zhou, Jia, Jiawei Xu, Rong Xu, et al. "Identifying and characterizing Stagonosporopsis cucurbitacearum causing spot blight on Pinellia ternata in China." PeerJ 10 (April 13, 2022): e13278. http://dx.doi.org/10.7717/peerj.13278.

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Background Pinellia ternata (Thunb.), a perennial herbal plant in the Araceae family, has great medicinal value and market demand. In August 2020, an outbreak of severe leaf spot blight disease resulted in a huge yield loss of P. ternata. It is necessary to isolate and identify the pathogens that cause spot blight on P. ternata. Methods In this study, we isolated and identified the pathogens by fulfilling Koch’s postulates. Disease samples with typical spot blight symptoms were collected and pathogens were isolated from the diseased tissues. The pathogen was identified based on its biological

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Mulyati, N., Y. G. M. Bonawati, R. R. Noor, M. F. Ulum, and Jakaria. "ND1 Gene Analysis in Beef Cattle for Sustainable Genetic Resource Management." IOP Conference Series: Earth and Environmental Science 1484, no. 1 (2025): 012003. https://doi.org/10.1088/1755-1315/1484/1/012003.

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Abstract The mitochondrial ND1 gene encodes the NADH-ubiquinone oxidoreductase chain and can be considered a candidate gene associated with adaptation to high-altitude environments. This study aimed to identify sequence variations in the ND1 gene and to assess the genetic relationships among Bali, PO, and Wagyu-Bali crossbred cattle. A total of 12 samples were analyzed, consisting of 2 Bali, 5 PO, and 5 Wagyu-Bali crossbred cattle. Identification of ND1 gene SNPs and phylogenetic analysis were performed using the Neighbor-Joining method with 1,000 bootstrap replicates in the MEGA7 software. Ge

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Korniyenko, M. V., та І. М. Кsyonz. "Визначення поліморфних варіантів фрагментів ДНК збудників хламідійних інфекцій сільськогосподарських тварин". Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 19, № 73 (2017): 66–70. http://dx.doi.org/10.15421/nvlvet7314.

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Under the currently existing classification, adopted at the Second European Symposium «Animal Chlamydioses and Zoonotic Implications (EMAC-2)», Chlamydia pathogens of animals and humans are intracellular gram-negative bacteria belonging to Chlamydiales order, Chlamydiaseae family, Chlamydia genus. The above mentioned genus includes 11 species: C. abortus, C. avium, C. caviae, C. felis, C. gallinacea, S. muridarum, C. pecorum, C. pneumoniae, C. psittaci, C. suis and C. trachomatis, 10 of them being pathogenic for animals and Chlamydia trachomatis being exclusively human Chlamydiosis agent. Deve

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Hartanti, Anastasia Tatik, Amelia Raharjo, and Agustin Wydia Gunawan. "Rhizopus Rotting on Agricultural Products in Jakarta." HAYATI Journal of Biosciences 27, no. 1 (2020): 37. http://dx.doi.org/10.4308/hjb.27.1.37.

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Agricultural products may easily perish if they are not well taken care of during postharvest treatment. One of the main causes in damaged products is the biological contamination of pathogenic fungi, such as Rhizopus spp. that gives rise to Rhizopus rot symptoms. The purpose of this research was to isolate Rhizopus spp. from different agricultural products showing Rhizopus rot symptoms as well as to identify them. Rhizopus pure cultures were isolated on potato dextrose agar. Identification was done through molecular techniques using PhytopureTM DNA Extraction Kit and Maxwell® RSC Plant DNA Ki

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Espiritu, Hector M., Lovelia L. Mamuad, Seon-ho Kim, et al. "Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing." Animals 10, no. 10 (2020): 1798. http://dx.doi.org/10.3390/ani10101798.

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This study analyzed the diversity and phylogenetic relationship of the microbiome of bovine digital dermatitis (BDD) lesions and normal skin from cattle foot by using 16S rRNA amplicon sequencing. Three BDD samples and a normal skin sample were pre-assessed for analysis. The Illumina Miseq platform was used for sequencing and sequences were assembled and were categorized to operational taxonomic units (OTUs) based on similarity, then the core microbiome was visualized. The phylogeny was inferred using MEGA7 (Molecular evolutionary genetics analysis version 7.0). A total of 129 and 185 OTUs wer

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Marnis, Huria, Evi Tahapari, and Jadmiko Darmawan. "GENETIC PERFORMANCE OF THE STRIPED CATFISH (Pangasianodon hypophthalmus Sauvage, 1878) POPULATION DERIVED FROM SELECTIVE BREEDING." Indonesian Aquaculture Journal 14, no. 1 (2019): 1. http://dx.doi.org/10.15578/iaj.14.1.2019.1-5.

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Selective breeding to improve growth trait in striped catfish (Pangasianodon hypophthalmus) need genetic performance data. This study was carried out to evaluate genetic performance of different generations of the striped catfish population derived from selective breeding. Fifty fish of each population from four generations selected striped catfish was analyzed using five microsatellite loci (Pg-1, Pg-2, Pg-3, Pg-13, and Pg-14). Microsatellite allele data were analyzed using Microsoft Excel, Arlequin, and Fstat software. A neighborhood joining dendrogram was constructed based on Nei’s distance

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Tsekhmister, H., E. Kopilov,, O. Nadkernychna, and A. Kyslynska. "Phylogeny of Plectosphaerella melonis strain 502 and varietal sensitivity of cucumber plants." Agricultural Science and Practice 9, no. 1 (2022): 3–14. http://dx.doi.org/10.15407/agrisp9.01.003.

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Aim. To investigate the phylogenetic relations of P. melonis strain 502 and to study the varietal sensitivity of cu- cumber plants to P. melonis strain 502. Methods. DNA was extracted using the enzymatic lysis buffer. The PCR was conducted following White et al. protocol (1990). The obtained PCR-products were determined by sequencing on the automatic capillary sequencer Applied Biosystems ABI Prism 3130. The sequence of the gene 5.8S rRNA of P. melonis strain 502 was compared to the sequences from the GenBank database using the BLAST analysis. The phy- logenetic analysis was conducted by the n

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Khrabrova, L. A., N. V. Blohina, R. B. Chysyma, B. Z. Bazaron, and T. N. Khamiruev. "Assessment of mtDNA variability and phylogenetic relationships of Siberian local horse breeds." IOP Conference Series: Earth and Environmental Science 839, no. 5 (2021): 052009. http://dx.doi.org/10.1088/1755-1315/839/5/052009.

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Abstract Local horse breeds of Siberia have universal economic characteristics and good adaptive qualities. The most valuable gene pool of these breeds has been formed over the centuries, and is of considerable interest for study at the molecular genetic level. The aim of our research was to study the sequence of the hypervariable region of the D-loop of mtDNA in 88 horses of the Buraytskaya, Zabaikalskaya, Tuvinckaya, and Yakutskaya breeds. Analysis of the sequenced D-loop sequence of 530 bp was performed using the Neighbor-Joining method in combination with bootstrap analysis in the MEGA7 pr

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Kolli Guna Ranjan, Girija Sankar G, and Satyanarayana Raju DVV. "Isolation and Characterization of Vibrio parahaemolyticus in the hepatopancreas of cultured white pacific shrimp - Litopenaeus vannamei." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (2020): 797–805. http://dx.doi.org/10.26452/ijrps.v11i1.1897.

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Vibrio parahaemolyticus is the most common species among crustaceans, often causing various diseases and significant losses in aquaculture. Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. This species of bacteria is associated with gastrointestinal illness in humans and has been implicated in foodborne disease. The present study carried out, isolation and characterization of pathogenic bacterial flora isolated from the infected hepatopancreas of vannamei, obtained from various aquafarms in Andhra Pradesh,

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Kolli Guna Ranjan, Girija Sankar G, and Satyanarayana Raju D V V. "Isolation and Characterization of Commercial Probiotics." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (2020): 818–25. http://dx.doi.org/10.26452/ijrps.v11i1.1900.

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Commercial probiotics have bacteria which offer scope for specific treatment as a probiotic against diarrhoea, boosting immune response and relieving stress. Research in probiotics and aquaculture has been done to promote sustainable aquaculture. Studies on microbial ecology in aquaculture and their benefits on human systems are to be assessed. Hence, the present study was aimed at the isolation and characterization of these bacteria obtained from various commercial probiotics. The commercial probiotic samples were collected for isolation using MRS agar. The colonies were selected on the basis

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Zhao, Ning, Kai Li, Huifen Ma, Lianrong Hu, Yingxue Yang, and Ling Liu. "Molecular Characterization of Odorant-Binding Protein Genes Associated with Host-Seeking Behavior in Oides leucomelaena." International Journal of Molecular Sciences 25, no. 17 (2024): 9436. http://dx.doi.org/10.3390/ijms25179436.

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The identification of odorant-binding proteins (OBPs) involved in host location by Oides leucomelaena (O. leucomelaena Weise, 1922, Coleoptera, Galerucinae) is significant for its biological control. Tools in the NCBI database were used to compare and analyze the transcriptome sequences of O. leucomelaena with OBP and other chemosensory-related proteins of other Coleoptera insects. Subsequently, MEGA7 was utilized for OBP sequence alignment and the construction of a phylogenetic tree, combined with expression profiling to screen for candidate antennae-specific OBPs. In addition, fumigation exp

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Wahyudi, Didik, Ihda Ummu Aufa, and Lia Hapsari. "Phylogenetic analysis and molecular characterization of BBTV DNA-R of wild and cultivated banana isolates from East Java, Indonesia." Revista Facultad Nacional de Agronomía Medellín 76, no. 3 (2023): 10437–44. http://dx.doi.org/10.15446/rfnam.v76n3.104708.

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Banana bunchy top virus (BBTV) molecular detection and understanding its origin are important issues for mitigating future spread. The aim of this study was to molecularly detect BBTV infection and analyze the characteristic also phylogenetic of banana isolates from East Java Indonesia. Two BBTV asymptomatic wild bananas and two BBTV symptomatic banana cultivars were examined. PCR amplifications were accomplished using BBTV DNA-R primers for master replication initiation protein. Sequences evaluations were conducted in SeqScanner. Sequences identification was performed in nucleotide BLAST. Tra

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Waqar Mazhar, Muhammad, Hamza Ali, Javeria Umber, Mahrosh Anjam, Sara Batool, and Hassan Raffique. "In-silico structural and phylogenetic analysis of human beta globin gene family." International Journal of Immunology and Microbiology 1, no. 1 (2022): 1–8. http://dx.doi.org/10.55124/ijim.v1i1.156.

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There are 5 members of the human beta globin gene family. They have blood transportation function. There are some diseases related to the mutation in these gene. the common disease related to mutation these genes are sickle cell anemia, thalassemia etc. The structural and phylogenetic analysis is performed by using some bioinformatics tools. The gene sequence is retrieved and run under some tools to perform the specific analysis. The analysis performed include are similarity with other organism, determination of conserved domains and motifs, multiple sequence alignment, generation of phylogene

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SINGH, SANDEEP, RANBIR SINGH, and C. S. MUKHOPADHYAY. "PCR amplification, sequencing, and in silico analysis of holandric genes TSPYand SRY in Nili Ravi buffalo." Indian Journal of Animal Sciences 94, no. 5 (2024): 426–30. http://dx.doi.org/10.56093/ijans.v94i5.123881.

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The present work has been designed to study the evolutionary perspectives of two important holandric genes, namely, testis-specific protein, Y-encoded (TSPY), and sex determining region, Y-encoded (SRY) in Nili Ravi buffalo. These Y-chromosomal genes are responsible for male sex development. The evolutionary aspects of these genes have not been studied in the Nili Ravi buffaloes from India. A study was carried out on the peripheral blood samples of three adult Nili Ravi bulls belonging to university farm. DNA was extracted using the phenol-chloroform isoamyl alcohol (PCI) technique and reporte

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Shah, Aftab Ali, Mushtaq Ahmad, and Taqweem Ul-Haq. "Deciphering conserved identical sequences of mature miRNAs among six members of great apes." Zoosystematics and Evolution 94, no. 2 (2018): 401–8. http://dx.doi.org/10.3897/zse.94.28099.

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MicroRNAs (miRNAs) are a group of small RNA molecules which act as negative regulators of gene expression by controlling post-transcriptional regulation through binding to their corresponding mRNAs. Due to their small size, their nucleotide compositions are expected to be similar, but until now, the extent of similarity has not been reported in humans and their six phylogenetically closely related members of hominids. The present study allows direct comparison among six members of hominid species (Homosapiens, Gorillagorilla, Panpaniscus, Pongopygmaeus, Pantroglodytes and Symphalangussyndactyl

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Obuhov, Yu, K. Neverov, Yu Maleeva та M. Krickij. "ИССЛЕДОВАНИЕ ФОТОХИМИЧЕСКИХ СВОЙСТВ И ФИЛОГЕНИИ РАСТИТЕЛЬНЫХ БЕЛКОВ СЕМЕЙСТВА WSCP". EurasianUnionScientists 3, № 11(80) (2020): 38–43. http://dx.doi.org/10.31618/esu.2413-9335.2020.3.80.1118.

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Here we show the ability of the chlorophyll associated with proteins of the WSCP family (Water-Soluble Chlorophyll-binding Proteins) to photosensitize oxidative redox reactions. Irradiation with red light of the recombinant chlorophyll-protein complexes WSCP subclasses IIa and IIb in the presence of an electron donor (NADH) led to oxidation of the donor, i.e., these pigment-protein complexes showed photochemical activity. Meanwhile there was no photodestruction of chlorophyll associated with WSCP, which indicates the photocatalytic nature of the detected redox reaction. The kinetic constants o

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Ding, He, Tang, Du, and Wang. "Identification of Maize CC-Type Glutaredoxins That Are Associated with Response to Drought Stress." Genes 10, no. 8 (2019): 610. http://dx.doi.org/10.3390/genes10080610.

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Global maize cultivation is often adversely affected by drought stress. The CC-type glutaredoxin (GRX) genes form a plant-specific subfamily that regulate plant growth and respond to environmental stresses. However, how maize CC-type GRX (ZmGRXCC) genes respond to drought stress remains unclear. We performed a TBLASTN search to identify ZmGRXCCs in the maize genome and verified the identified sequences using the NCBI conservative domain database (CDD). We further established a phylogenetic tree using Mega7 and surveyed known cis-elements in the promoters of ZmGRXCCs using the PlantCARE databas

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E, Batmagnai, Ariunbold G, Erdenechimeg D, et al. "The result of propagation of sheep and goat pox viral vaccine strains into vero cells." Mongolian Journal of Agricultural Sciences 26, no. 01 (2019): 3–9. http://dx.doi.org/10.5564/mjas.v26i01.1190.

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Background: Sheep and goat pox viral disease, which affects negatively to our country’s economy by prevalence and infection, has high mortality and morbidity rate. Although our country manufactures sheep and goat pox viral vaccine using lamb’s testicle tissue in the Biocombinat (Bio-factory), in winter, there is high number of diseased animals, lamb testicle is scarce, therefore there is a need to produce cell-culture based sheep and goat pox vaccine. Materials and methods: Russian VNIIZJ strain type 2 sheep pox vaccine and Chinese goat pox live vaccine (serial number 010030) antigens we

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Arifudin Arifudin, Alwi Alwi, and Aris Munandar. "Pengaruh Earning Per Share (EPS) dan Dividen Per Share (DPS) Terhadap Harga Saham PT Indo Tambangraya Megah Tbk." Journal Economic Excellence Ibnu Sina 2, no. 3 (2024): 152–68. http://dx.doi.org/10.59841/excellence.v2i3.1712.

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This research aims to determine whether there is an influence of Earning Per Share (EPS) and Dividend Per Share (DPS) on the share price of PT Indo Tambangraya Megah Tbk. The research method used is associative research with a quantitative approach. The population used in this research is all of PT's financial reports. Indo Tambang Raya Mega Tbk. Since being listed on the IDX from 2007 to 2023, which is 17 years. The sample used in this research uses financial report data from PT. Indo Tambang Raya Mega Tbk. in the form of reports of net income, dividends and share prices, for 12 years from 20

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Tamura, K., J. Dudley, M. Nei, and S. Kumar. "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0." Molecular Biology and Evolution 24, no. 8 (2007): 1596–99. http://dx.doi.org/10.1093/molbev/msm092.

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Zezekalo, Viktoriya K., Konstantin F. Pochernyaev, Vasyl M. Voloshchuk, Liudmyla V. Zasukha, Natalia S. Shcherbakova, and Serhii M. Kulynych. "MULTIPLEX PCR ASSAY FOR CHLAMYDIA-LIKE BACTERIA DETECTION." Wiadomości Lekarskie 72, no. 5 (2019): 851–55. http://dx.doi.org/10.36740/wlek201905123.

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Introduction: Waddlia chondrophila and Parachlamydia acanthamoebae are well-known and best-studied representatives of Сhlamydia-related bacteria carrying a potential zoonotic threat. These bacteria are associated with miscarriage, ectopic pregnancy, diseases of the respiratory system in both humans and animals. Despite the importance of these Сhlamydia-like organisms for human medicine along with veterinary medicine, studies on their prevalence in Ukraine were not conducted due to the lack of available tests. The aim of our work was to create relatively cheap and easy method for detection Wadd

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Lozano, Carlos Hernando Parga, and Nohemí Esther Santodomingo Guerrero. "Model for the Study of Drug Hypersensitivity Based on Bucilamine and the HLA-DRB1*08:02 Allele in Colombian Amerindian Populations." Revista de Gestão Social e Ambiental 18, no. 6 (2024): e05743. http://dx.doi.org/10.24857/rgsa.v18n6-191.

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Backgrounds: Allergic diseases and hypersensitivity reactions are common disorders that in turn consist of an extensive genetic component in which the molecules of the Major Histocompatibility Complex (MHC) are included, which have certain alleles associated with the development of hypersensitivity to certain drugs, among which is the allele HLA-DRB1 * 08: 02 as a predisposing factor of hypersensitivity to Bucillamine; this drug is the starting point for the study of the relationship between hypersensitivity reactions to medications and the expression of certain alleles of MHC. Objective: Find

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Shah, Aftab Ali, Mushtaq Ahmad, and Taqweem Ul-Haq. "Deciphering conserved identical sequences of mature miRNAs among six members of great apes." Zoosystematics and Evolution 94, no. (2) (2018): 401–8. https://doi.org/10.3897/zse.94.28099.

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MicroRNAs (miRNAs) are a group of small RNA molecules which act as negative regulators of gene expression by controlling post-transcriptional regulation through binding to their corresponding mRNAs. Due to their small size, their nucleotide compositions are expected to be similar, but until now, the extent of similarity has not been reported in humans and their six phylogenetically closely related members of hominids. The present study allows direct comparison among six members of hominid species (Homo sapiens, Gorilla gorilla, Pan paniscus, Pongo pygmaeus, Pan troglodytes and Symphalangus syn

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Coila Añasco, Pedro Ubaldo, Julio César Bernabé Ortiz, and Domingo Alberto Ruelas. "BACTERIAS ENDOFÍTICAS DE ELODEA POTAMOGETON (“LLACHU”) DEL LAGO TITICACA." SCIENTIARVM 1, no. 1 (2015): 35–38. http://dx.doi.org/10.26696/sci.epg.0128.

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ABTRACT: The “llachu” (Elodea potamogeton) is an aquatic plant from Lake Titicaca, food for the cattle of the circumlacustrine ring. Some areas of the lake have been eutrophied by pollution; even so, the plant grows. Being one of the survival mechanisms, the symbiotic interaction with microorganisms, the objective of the study was to isolate and molecularly characterize some endophytic bacteria of the plant. For isolation, the samples were washed and disinfected with 70% alcohol, 0.5% sodium hypochlorite and 80% Tween; then, it was homogenized and an aliquot seeded in Luria-Bertani (LB) medium

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Kwaśnik, Małgorzata, Ilona M. Góra, Jan F. Żmudziński, Jerzy Rola, Mirosław P. Polak, and Wojciech Rożek. "Genetic analysis of the M gene of equine influenza virus strains isolated in Poland, in the context of the Asian-like group formation." Journal of Veterinary Research 62, no. 4 (2018): 405–12. http://dx.doi.org/10.2478/jvetres-2018-0057.

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AbstractIntroduction:Traditionally, evolutionary analysis of equine influenza virus (EIV) is based on the HA gene. However, the specificity of the influenza virus enables the classification of viral strains into different phylogenetic groups, depending on the gene being analysed. The aim of the study was to analyse phylogenetic paths of EIV based on M gene with reference to the HA gene.Material and Methods:M gene of Polish isolates has been sequenced and analysed along with all M sequences of EIV available in GenBank database. Phylogenetic analysis was performed using BioEdit, ClustalW, and ME

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Andriyono, S., A. Damora, and A. A. Hidayani. "Identificación molecular y reconstrucción filogenética de peces marinos de la estación de desembarque de pescado de Kutaradja, Banda Aceh." Archivos de Zootecnia 73, no. 283 (2024): 124–32. https://doi.org/10.21071/az.v73i283.5851.

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Se espera que el gran potencial de los recursos marinos que posee la provincia de Banda Aceh se utilice de manera óptima. La identificación completa de los recursos de peces marinos es un requisito importante para apoyar su utilización y preservación en la provincia de Banda Aceh. En este estudio, se lleva a cabo un enfoque de identificación molecular, además de realizar una identificación morfológica que ha sido comúnmente utilizada. En este estudio de identificación molecular, el ADN objetivo es la región de la subunidad I de la citocromo c oxidasa (COI) ubicada en las mitocondrias. El métod

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Poleshchuk, E. M., D. N. Tagakova, G. N. Sidorov, T. S. Orlova, N. S. Gordeiko, and A. Zh Kaisarov. "Lethal cases of lyssavirus encephalitis in humans after contact with bats in the Russian Far East in 2019–2021." Problems of Virology 68, no. 1 (2023): 45–58. http://dx.doi.org/10.36233/0507-4088-156.

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Introduction. On the territory of Russia four species of lyssaviruses (genus Lyssavirus) were identified, three of them caused human deaths. The aim of work: to characterize fatal cases in humans after contacts with bats in the Far East in 20182021 and to perform typing of isolated pathogens. Materials and methods. Lyssavirus infection was confirmed in samples of sectional material from people who died in the Amur Region in 2019, in the Primorsky Krai in 2019 and 2021. Diagnostics was performed by fluorescent antibody test (FAT) and RT-PCR using diagnostic kits of domestic producti

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Khrabrova, Liudmila A., Anna A. Nikolaeva, Nina V. Blohina, and Sergey I. Sorokin. "GENETIC DIVERSITY OF MITOCHONDRIAL DNA HAPLOGROUPS IN THE DON HORSE BREED." Siberian Journal of Life Sciences and Agriculture 15, no. 4 (2023): 278–90. http://dx.doi.org/10.12731/2658-6649-2023-15-4-278-290.

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The Don breed of horses, which became famous in Russia already in the middle of the 18th century, was obtained by long-term crossing of nomadic horses with cultured oriental breeds. Due to the reduction in the number of Don queens to 200 broodmares, the research of the genetic characteristics of this breed is of particular relevance. The purpose of the research was to study the matrilineal structure of the Don horse breed based on phylogenetic analysis of 530 nucleotide pairs [n.p.] of the hypervariable section of the D-loop of mitochondrial DNA [mtDNA] in 26 mares representing the main female

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Kotlyarov, Stanislav N., and Anna A. Kotlyarova. "Role of lipid metabolism and systemic inflammation in the development of atherosclerosis in animal models." I.P. Pavlov Russian Medical Biological Herald 29, no. 1 (2021): 134–46. http://dx.doi.org/10.23888/pavlovj2021291134-146.

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Systemic inflammation makes a significant contribution to the pathogenesis of atherosclerosis and has been the subject of numerous studies. Works aiming to analyze the mechanisms of atherosclerosis development often include experiments on animals. A primary task of such research is the characterization, justification, and selection of an adequate model. Aim. To evaluate the peculiarities of lipid metabolism and systemic inflammation in chronic obstructive pulmonary disease (COPD) in the development of atherosclerosis in animal models. Materials and Methods. Analyses of cross-links

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Mindiarova, V. O., D. S. Savenkova, Yu O. Filippova, and A. V. Milovanov. "ANALYSIS OF THE GENETIC MATERIAL OF NATIVE GRAPE VARIETIES OF THE RUSSIAN AMPELOGRAPHIC COLLECTION." Bulletin of Agrarian Science 5, no. 86 (2020): 51–58. http://dx.doi.org/10.17238/issn2587-666x.2020.5.51.

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One of the main places in the selection of grapes today belongs to native varieties. Native varieties possess characteristics valuable in economic relations, such as a high-quality berry crop, from which it is subsequently possible to obtain the highest quality wine, as well as resistance to harsh climatic conditions. However, before starting breeding work, it is necessary to conduct various pre-breeding studies, including genetic analysis. This type of analysis is the most informative of all, because it gives the most complete picture of all the genetic predispositions of any organism, as wel

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Al-Zahrani, Hassan S., Tarek A. A. Moussa, Hameed Alsamadany, Rehab M. Hafez, and Michael P. Fuller. "Phylogenetic and Expression Studies of Small GTP-Binding Proteins in Solanum lycopersicum Super Strain B." Plants 11, no. 5 (2022): 641. http://dx.doi.org/10.3390/plants11050641.

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This investigation involved a comparative analysis of the small GTPase superfamily in S. lycopersicum super strain B compared to their analogues in leguminous and other non-leguminous species. The small GTPases superfamily members were recognized by tBLASTn searches. The sequences of amino acid were aligned using Clustal Omega and the analysis of phylogeny was performed with the MEGA7 package. Protein alignments were applied for all studied species. Three-dimensional models of RABA2, ROP9, and ROP10 from Solanum lycopersicum “Super strain B” were performed. The levels of mRNA of the Rab, Arf,

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Ding, Shuangcheng, Xin Feng, Hewei Du, and Hongwei Wang. "Genome-wide analysis of maize OSCA family members and their involvement in drought stress." PeerJ 7 (April 11, 2019): e6765. http://dx.doi.org/10.7717/peerj.6765.

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Background Worldwide cultivation of maize is often impacted negatively by drought stress. Hyperosmolality-gated calcium-permeable channels (OSCA) have been characterized as osmosensors in Arabidopsis. However, the involvement of members of the maize OSCA (ZmOSCA) gene family in response to drought stress is unknown. It is furthermore unclear which ZmOSCA gene plays a major role in genetic improvement of drought tolerance in Maize. Methods We predicted the protein domain structure and transmembrane regions by using the NCBI Conserved Domain Database database and TMHMM server separately. The phy

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Hidayani, Andi Aliah, Asmi Citra Malina A. R. Tasakka, Widyastuti Umar, Md Jobaidul Alam, Amit Kumer Neogi, and Sapto Andriyono. "Low Genetic Diversity Study on Leopard Coral Grouper Plectropomus leopardus (Perciformes: Serranidae) from Sulawesi, Indonesia." Jurnal Ilmiah Perikanan dan Kelautan 14, no. 2 (2022): 349–59. http://dx.doi.org/10.20473/jipk.v14i2.32815.

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Highlight ResearchThe leopard coral grouper Plectropomus leopardus was identified and analysed based on molecular approach.Genetic diversity within two regions in Gorontalo, Sulawesi successfully performed using connectivity analysis.Three haplotypes of Plectropomus leopardus from two region in Gorontalo as one of economical important marine fish species. AbstractBar-cheek coral trout (P. leopardus) is the flagship of the grouper in the live fish market in Asia. Unfortunately, the potential of the grouper is still partly produced from natural catches. Even though hybridisation activities have

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Tanni, Afroza Akter, Md Mahbub Hasan, Nahid Sultana, Wazir Ahmed, and Adnan Mannan. "Prevalence and molecular characterization of antibiotic resistance and associated genes in Klebsiella pneumoniae isolates: A clinical observational study in different hospitals in Chattogram, Bangladesh." PLOS ONE 16, no. 9 (2021): e0257419. http://dx.doi.org/10.1371/journal.pone.0257419.

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Objective This study was performed to investigate the prevalence of multidrug resistance and molecular characterization of Klebsiella pneumoniae (KPN) from clinical isolates in the southern region of Bangladesh. Additional analysis of the prevalence of blaNDM-1, blaSHV-11, uge genes of KPN was also carried out among these clinical isolates. Method The study was carried out using 1000 clinical isolates collected from two different hospitals of Chattogram. A drug susceptibility test was performed by the disk diffusion method to detect KPN’s response to 16 antibiotics. The presence of antibiotic-

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Sasongko, N. D., A. Yuniati, and L. A. Gayo Billmora. "Genetic profiles of three Cinchona species in Junghuhn Natural Reserve, Indonesia." SABRAO Journal of Breeding and Genetics 53, no. 4 (2021): 697–709. http://dx.doi.org/10.54910/sabrao2021.53.4.12.

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Cinchona species were widely used as ancient medicines for different diseases because they contain the active component quinine and its derivatives. However, studies on the molecular aspects of cinchona, including its genetic diversity, have not been reported because most previous works focused on the administration of the antimalarial cinchona alkaloid. Quinine is also being tested as alternative compound for the treatment of Covid-19. The Junghuhn Natural Reserve in Indonesia contains three different types of cinchona plants, namely, Cinchona calisaya, Cinchona pubescens, and Cinchona sp. Gi

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Mokaddas, Eiman, Suhail Ahmad, and Noura Al-Mutairi. "759. Molecular Characterization and Epidemiology of Multidrug-Resistant Mycobacterium tuberculosis (MDR-TB) and Identification of Possible Cases of Local Transmission of MDR-TB in Kuwait." Open Forum Infectious Diseases 5, suppl_1 (2018): S272. http://dx.doi.org/10.1093/ofid/ofy210.766.

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Abstract Background Increasing incidence of multidrug-resistant tuberculosis (MDR-TB) is hampering efforts to control TB. Kuwait is a low (25/100,000) TB incidence country and ~1% of Mycobacterium tuberculosis strains are resistant to rifampin, RIF and isoniazid, INH (MDR-TB). Analysis of resistance conferring mutations in seven genes was combined with spoligotyping for detecting local transmission of MDR-TB in Kuwait. Methods MDR-TB strains (n = 131) from 88 TB patients and 50 susceptible strains were used. Susceptibility testing was done by MGIT 960 system, gMTBDRplus assay and PCR-sequencin

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Gradoboeva, E. A., Zh S. Tyulko, A. V. Fadeev, A. G. Yakimenko, and V. V. Yakimenko. "Comparative Analysis of the Diversity of SARS-CoV-2 Lines Circulating in Omsk Region in 2020–2022." Epidemiology and Vaccinal Prevention 21, no. 6 (2023): 24–33. http://dx.doi.org/10.31631/2073-3046-2022-6-24-33.

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Relevance. To date, no detailed analysis of the variants of the pathogen circulating at different times on the territory of the Omsk region has been carried out.Aim. Comparative analysis of the diversity of circulating variants of SARSCoV-2 based on molecular genetic data, determine the lines and time of their appearance, compare the data obtained with data from the GISAID database.Materials and methods. Genomewide sequencing of 222 primary and 5 culture (passages on Vero E6 and SPEV cell cultures) samples of SARS-CoV-2 from the Omsk region, collected from April 2020 to February 2022, on

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Hajimohammadi, B., G. Eslami, L. Manafi, SS Athari, and MJ Boozhmehrani. "Sarcocystis sinensis in slaughtered cattle from Central of Iran." Journal of the Hellenic Veterinary Medical Society 73, no. 2 (2022): 4147–52. http://dx.doi.org/10.12681/jhvms.26793.

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Sarcocystis is a food borne parasite as an intracellular parasite with two-host life cycle. Humans serve as either intermediate or definitive hosts among different species. Sarcocystosis is a very important disease because of its widespread prevalence with an impact on production and animal health. We aimed to assess S. sinensis in cattle from the desert area of Iran, using conventional PCR, targeting 18S rRNA. Also, the phylogenic analysis was done to mention isolate and the related ones in Genbank.From the 108 beef samples of heart, esophagus, intercostal muscle, diaphragm, and tongue harbor

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Shazia, Irfan, Shabbir Ahmed Rana Shajahan, Ali Sani Imran, et al. "Characterization of the Complete Chloroplast Genome Sequence of Juniperus polycarpos K. Koch (Cupressaceae), from Ziarat, Pakistan." Indian Journal of Science and Technology 14, no. 36 (2021): 2806–14. https://doi.org/10.17485/IJST/v14i36.151.

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Abstract <strong>Objectives:</strong> To broaden the genetic information base of Juniperus and resolve phylogeny of Juniperus polycarpos through sequencing and characterization of its chloroplast genome. <strong>Methods:</strong> The chloroplast (cp) genome of J. polycarpos was sequenced and assembled using the Next-Generation Sequencing pairedend reads platform of BGISEQ-500 and annotated using CpGAVAS. The phylogenetic analysis was performed in MEGA7.<strong> Findings:</strong> Here, we report the complete cp genome sequence of J. polycarpos. The cp genome size is 12

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Zhao, Xinyang, Ngoc-Tung Tran, Dewang Zhou, Hairui Su, Li Zhang, and Tim M. Townes. "Transcription Activation of an Antisense Long Noncoding RNA (lnc-Mega1) of RBM15 By RUNX1 Enhances Megakaryocyte Differentiation." Blood 124, no. 21 (2014): 4305. http://dx.doi.org/10.1182/blood.v124.21.4305.4305.

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Abstract RBM15 encodes an RNA binding protein, which regulates self-renewal and differentiation of hematopoietic stem cells. RBM15 knock-out promotes the proliferation of Mk progenitor cells but blocks the maturation of Mk progenitor cells as measured by polyploidy. RBM15-MKL1 fusion causes pediatric acute megakaryoblastic leukemia without Down’s syndrome. Here, we cloned a long non-coding RNA (we called Lnc-Mega1), which is transcribed in the opposite direction of the human RBM15 gene. Lnc-Mega1 is not transcribed in mice in the RBM15 locus. Blast search also indicates there are no homologues

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Costa Neto, Pedro Leão da. "SOBRE A RECEPÇÃO DAS OBRAS DE MARX E ENGELS ATÉ 1989." Revista Ideação 1, no. 39 (2019): 149. http://dx.doi.org/10.13102/ideac.v1i39.4569.

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O objetivo do presente artigo e, por um lado, analisar a história da constituição docorpus das obras de Karl Marx e Friedrich Engels e das sucessivas tentativas de publicação das suas Obras Completas (MEGA, MEW, MEGA2), procurando identificar os vários obstáculos de natureza política e teórica que retardaram a sua realização. Por outro lado, procura-se, igualmente, evidenciar a relação deste processo com a história da difusão e recepção das obras de Marx e Engels.

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CERQUEIRA, HUGO E. A. DA GAMA. "Breve história da edição crítica das obras de Karl Marx." Revista de Economia Política 35, no. 4 (2015): 825–44. http://dx.doi.org/10.1590/0101-31572015v35n04a08.

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RESUMOEste artigo discute as tentativas de publicar uma edição histórico-crítica das obras de Karl Marx, o Marx-Engels Gesamtausgabe (MEGA): a primeira, que foi liderada por David Riazanov na décadas de 1920 e 1930, e o segundo, a MEGA2, projeto que começou na década de 1970 e ainda está em curso de publicação. O artigo apresenta essas duas edições e discute o seu impacto sobre a interpretação do pensamento econômico e filosófico de Marx.

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Idris, Abeer Babiker, Hadeel Gassim Hassan, Maryam Atif Salaheldin Ali, et al. "Molecular Phylogenetic Analysis of 16S rRNA Sequences Identified Two Lineages of Helicobacter pylori Strains Detected from Different Regions in Sudan Suggestive of Differential Evolution." International Journal of Microbiology 2020 (October 27, 2020): 1–12. http://dx.doi.org/10.1155/2020/8825718.

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Background. Helicobacter pylori (H. pylori) is ubiquitous among humans and one of the best-studied examples of an intimate association between bacteria and humans. Phylogeny and Phylogeography of H. pylori strains are known to mirror human migration patterns and reflect significant demographic events in human prehistory. In this study, we analyzed the molecular evolution of H. pylori strains detected from different tribes and regions of Sudan using 16S rRNA gene and the phylogenetic approach. Materials and methods. A total of 75 gastric biopsies were taken from patients who had been referred f

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Stapleton, Patrick, Alireza Eshaghi, Eddie Chong-King, et al. "Molecular Investigation of an Ontario Mumps Outbreak using Whole Genome Sequencing." Open Forum Infectious Diseases 4, suppl_1 (2017): S359. http://dx.doi.org/10.1093/ofid/ofx163.870.

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Abstract Background In early 2017 an outbreak of Mumps virus affected over 100 individuals in the province of Ontario, concurrent with multiple mumps virus outbreaks across North America. Traditional genotyping of mumps outbreaks relies on sequencing a portion of the small hydrophobic (SH) gene, but has limited capability to distinguish between strains of the same genotype. Most mumps cases in Ontario in recent years are of genotype G. We used a novel whole genome sequencing (WGS) protocol to perform a molecular epidemiological investigation of the outbreak. Methods Throat (n = 5) and buccal (

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