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Journal articles on the topic 'Membrane implicite'

Author: Grafiati

Published: 25 May 2024

Last updated: 31 July 2025

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1

Amrouche, Fethia, Bouziane Mahmah, Maiouf Belhamel, and Hocine Benmoussa. "Modélisation d’une pile à combustible PEMFC alimentée directement en hydrogène-oxygène et validation expérimentale." Journal of Renewable Energies 8, no. 2 (2005): 109–21. http://dx.doi.org/10.54966/jreen.v8i2.856.

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La pile à combustible (PAC) est connue depuis longtemps comme un convertisseur d’hydrogène en énergie (électrique + thermique) possédant de très bons rendements, les recherches sur cette technologie se développent partout dans le monde de manière considérable. Les raisons sont bien connues: la réponse aux contraintes environnementales, aux problèmes posés par la production centralisée d’électricité, la nécessité d’avoir des alternatives énergétiques (vecteur hydrogène) et certaines exigences technologiques spécifiques telles que les applications spatiales, sous-marines, électroniques portables

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Tian, Ye, Charles Schwieters, Stanley Opella, and Francesca Marassi. "NMR-Restrained Structure Calculations of Membrane Proteins in Implicit Lipid Bilayer Membranes." Biophysical Journal 108, no. 2 (2015): 251a. http://dx.doi.org/10.1016/j.bpj.2014.11.1389.

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3

Sáenz, James P., Daniel Grosser, Alexander S. Bradley, et al. "Hopanoids as functional analogues of cholesterol in bacterial membranes." Proceedings of the National Academy of Sciences 112, no. 38 (2015): 11971–76. http://dx.doi.org/10.1073/pnas.1515607112.

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The functionality of cellular membranes relies on the molecular order imparted by lipids. In eukaryotes, sterols such as cholesterol modulate membrane order, yet they are not typically found in prokaryotes. The structurally similar bacterial hopanoids exhibit similar ordering properties as sterols in vitro, but their exact physiological role in living bacteria is relatively uncharted. We present evidence that hopanoids interact with glycolipids in bacterial outer membranes to form a highly ordered bilayer in a manner analogous to the interaction of sterols with sphingolipids in eukaryotic plas

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Zelhof, Andrew C., Hong Bao, Robert W. Hardy, Azam Razzaq, Bing Zhang, and Chris Q. Doe. "DrosophilaAmphiphysin is implicated in protein localization and membrane morphogenesis but not in synaptic vesicle endocytosis." Development 128, no. 24 (2001): 5005–15. http://dx.doi.org/10.1242/dev.128.24.5005.

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Amphiphysin family members are implicated in synaptic vesicle endocytosis, actin localization and one isoform is an autoantigen in neurological autoimmune disorder; however, there has been no genetic analysis of Amphiphysin function in higher eukaryotes. We show that Drosophila Amphiphysin is localized to actin-rich membrane domains in many cell types, including apical epithelial membranes, the intricately folded apical rhabdomere membranes of photoreceptor neurons and the postsynaptic density of glutamatergic neuromuscular junctions. Flies that lack all Amphiphysin function are viable, lack a

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Petrie, Emma J., Richard W. Birkinshaw, Akiko Koide, et al. "Identification of MLKL membrane translocation as a checkpoint in necroptotic cell death using Monobodies." Proceedings of the National Academy of Sciences 117, no. 15 (2020): 8468–75. http://dx.doi.org/10.1073/pnas.1919960117.

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The necroptosis cell death pathway has been implicated in host defense and in the pathology of inflammatory diseases. While phosphorylation of the necroptotic effector pseudokinase Mixed Lineage Kinase Domain-Like (MLKL) by the upstream protein kinase RIPK3 is a hallmark of pathway activation, the precise checkpoints in necroptosis signaling are still unclear. Here we have developed monobodies, synthetic binding proteins, that bind the N-terminal four-helix bundle (4HB) “killer” domain and neighboring first brace helix of human MLKL with nanomolar affinity. When expressed as genetically encode

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Parodi, Emily M., Crystal S. Baker, Cayla Tetzlaff, Sasha Villahermosa, and Linda S. Huang. "SPO71 Mediates Prospore Membrane Size and Maturation in Saccharomyces cerevisiae." Eukaryotic Cell 11, no. 10 (2012): 1191–200. http://dx.doi.org/10.1128/ec.00076-12.

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ABSTRACT The mechanisms that control the size and shape of membranes are not well understood, despite the importance of these structures in determining organelle and cell morphology. The prospore membrane, a double lipid bilayer that is synthesized de novo during sporulation in S. cerevisiae , grows to surround the four meiotic products. This membrane determines the shape of the newly formed spores and serves as the template for spore wall deposition. Ultimately, the inner leaflet of the prospore membrane will become the new plasma membrane of the cell upon germination. Here we show that Spo71

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Mc Dermott, Ray, Umit Ziylan, Danièle Spehner, et al. "Birbeck Granules Are Subdomains of Endosomal Recycling Compartment in Human Epidermal Langerhans Cells, Which Form Where Langerin Accumulates." Molecular Biology of the Cell 13, no. 1 (2002): 317–35. http://dx.doi.org/10.1091/mbc.01-06-0300.

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Birbeck granules are unusual rod-shaped structures specific to epidermal Langerhans cells, whose origin and function remain undetermined. We investigated the intracellular location and fate of Langerin, a protein implicated in Birbeck granule biogenesis, in human epidermal Langerhans cells. In the steady state, Langerin is predominantly found in the endosomal recycling compartment and in Birbeck granules. Langerin internalizes by classical receptor-mediated endocytosis and the first Birbeck granules accessible to endocytosed Langerin are those connected to recycling endosomes in the pericentri

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8

Wilson, D. W., S. W. Whiteheart, M. Wiedmann, M. Brunner, and J. E. Rothman. "A multisubunit particle implicated in membrane fusion." Journal of Cell Biology 117, no. 3 (1992): 531–38. http://dx.doi.org/10.1083/jcb.117.3.531.

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The N-ethylmaleimide sensitive fusion protein (NSF) is required for fusion of lipid bilayers at many locations within eukaryotic cells. Binding of NSF to Golgi membranes is known to require an integral membrane receptor and one or more members of a family of related soluble NSF attachment proteins (alpha-, beta-, and gamma-SNAPs). Here we demonstrate the direct interaction of NSF, SNAPs and an integral membrane component in a detergent solubilized system. We show that NSF only binds to SNAPs in the presence of the integral receptor, resulting in the formation of a multisubunit protein complex

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Boyd, RB, JP Burke, J. Atkin, VW Thompson, and JF Nugent. "Significance of capillary basement membrane changes in diabetes mellitus." Journal of the American Podiatric Medical Association 80, no. 6 (1990): 307–13. http://dx.doi.org/10.7547/87507315-80-6-307.

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Diabetes mellitus is a disease in which the capillary basement membranes are substantially altered. This diabetic microangiopathy is characterized by a thickening of the basement membrane and changes in its permeability characteristic due to a disturbance in the production and distribution of its functional components. Glucose metabolism and insulin imbalance have been implicated in these basement membrane modifications. The authors describe normal capillary basement membrane architecture and then discuss how pathologic changes caused by diabetes mellitus are related to diabetic foot pathology

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GILLOOLY, David J., Anne SIMONSEN, and Harald STENMARK. "Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins." Biochemical Journal 355, no. 2 (2001): 249–58. http://dx.doi.org/10.1042/bj3550249.

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PtdIns3P is a phosphoinositide 3-kinase product that has been strongly implicated in regulating membrane trafficking in both mammalian and yeast cells. PtdIns3P has been shown to be specifically located on membranes associated with the endocytic pathway. Proteins that contain FYVE zinc-finger domains are recruited to PtdIns3P-containing membranes. Structural information is now available concerning the interaction between FYVE domains and PtdIns3P. A number of proteins have been identified which contain a FYVE domain, and in this review we discuss the functions of PtdIns3P and its FYVE-domain-c

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Seychell, Raina Marie, Adam El Saghir, Gianluca Farrugia, and Neville Vassallo. "Coenzyme Q10 Enhances Resilience of Mitochondrial-Like Membranes Against Amyloidogenic Peptides." Membranes 15, no. 5 (2025): 148. https://doi.org/10.3390/membranes15050148.

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Mitochondria possess a double-membrane envelope which is susceptible to insult by pathogenic intracellular aggregates of amyloid-forming peptides, such as the amyloid-beta (1-42) (Aβ42) peptide and the human islet amyloid polypeptide (hIAPP). The molecular composition of membranes plays a pivotal role in regulating peptide aggregation and cytotoxicity. Therefore, we hypothesized that modifying the physicochemical properties of mitochondrial model membranes with a small molecule might act as a countermeasure against the formation of, and damage by, membrane-active amyloid peptides. To investiga

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He, Yi, Lidia Prieto, and Themis Lazaridis. "Electrostatic Interactions between Antimicrobial Peptides and Anionic Membranes: Insights from an Implicit Membrane Model." Biophysical Journal 100, no. 3 (2011): 497a. http://dx.doi.org/10.1016/j.bpj.2010.12.2914.

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Bean, Björn D. M., Samantha K. Dziurdzik, Kathleen L. Kolehmainen, et al. "Competitive organelle-specific adaptors recruit Vps13 to membrane contact sites." Journal of Cell Biology 217, no. 10 (2018): 3593–607. http://dx.doi.org/10.1083/jcb.201804111.

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The regulated expansion of membrane contact sites, which mediate the nonvesicular exchange of lipids between organelles, requires the recruitment of additional contact site proteins. Yeast Vps13 dynamically localizes to membrane contacts that connect the ER, mitochondria, endosomes, and vacuoles and is recruited to the prospore membrane in meiosis, but its targeting mechanism is unclear. In this study, we identify the sorting nexin Ypt35 as a novel adaptor that recruits Vps13 to endosomal and vacuolar membranes. We characterize an interaction motif in the Ypt35 N terminus and identify related

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14

Saller, Manfred J., Zht Cheng Wu, Jeanine de Keyzer, and Arnold J. M. Driessen. "The YidC/Oxa1/Alb3 protein family: common principles and distinct features." Biological Chemistry 393, no. 11 (2012): 1279–90. http://dx.doi.org/10.1515/hsz-2012-0199.

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Abstract The members of the YidC/Oxa1/Alb3 protein family are evolutionary conserved in all three domains of life. They facilitate the insertion of membrane proteins into bacterial, mitochondrial, and thylakoid membranes and have been implicated in membrane protein folding and complex formation. The major classes of substrates are small hydrophobic subunits of large energy-transducing complexes involved in respiration and light capturing. All YidC-like proteins share a conserved membrane region, whereas the N- and C-terminal regions are diverse and fulfill accessory functions in protein target

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Chae, Gyung Joon, Sang Bae Lee, Ui Won Jung, Yong Keun Lee, Chong Kwan Kim, and Seong Ho Choi. "The Effects of Antibiotics Blended Chitosan Membranes on the Calvarial Critical Size Defect in Sprague Dawley Rats." Key Engineering Materials 342-343 (July 2007): 857–60. http://dx.doi.org/10.4028/www.scientific.net/kem.342-343.857.

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The purpose of this study was to evaluate the osteogenesis of tetracycline blended chitosan membranes on the calvarial critical size defect in Sprague Dawley. An 8 mm surgical defect was created with a trephine bur in the area of the midsagittal suture. Forty rats were divided into four groups: negative control group, positive control group and two experimental groups. Three types of membranes were made and a comparative study was done. One type of non-woven membrane was made by chitosan for positive control. The other two types of non-woven membranes were made by immersing non-woven chitosan

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16

Titorenko, Vladimir I., and Richard A. Rachubinski. "Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p." Journal of Cell Biology 150, no. 4 (2000): 881–86. http://dx.doi.org/10.1083/jcb.150.4.881.

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Two AAA family ATPases, NSF and p97, have been implicated in membrane fusion during assembly and inheritance of organelles of the secretory pathway. We have now investigated the roles of AAA ATPases in membrane fusion during assembly of the peroxisome, an organelle outside the classical secretory system. Here, we show that peroxisomal membrane fusion in the yeast Yarrowia lipolytica requires two AAA ATPases, Pex1p and Pex6p. Release of membrane- associated Pex1p and Pex6p drives the asymmetric priming of two fusion partners. The next step, peroxisome docking, requires release of Pex1p from one

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17

Jost, M., D. Zeuschner, J. Seemann, K. Weber, and V. Gerke. "Identification and characterization of a novel type of annexin-membrane interaction: Ca2+ is not required for the association of annexin II with early endosomes." Journal of Cell Science 110, no. 2 (1997): 221–28. http://dx.doi.org/10.1242/jcs.110.2.221.

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Annexin II, a member of a family of Ca2+ and membrane binding proteins, has been implicated in regulating membrane organization and membrane transport during endocytosis and Ca2+ regulated secretion. To characterize the mechanistic aspects of the annexin. II action we studied parameters which determine the endosomal association of annexin II. Immunoblot analysis of subcellular membrane fractions prepared from BHK cells in the presence of a Ca2+ chelating agent reveals that annexin II remains associated with endosomal membranes under such conditions. This annexin II behaviour is atypical for th

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Hitt, A. L., J. H. Hartwig, and E. J. Luna. "Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium." Journal of Cell Biology 126, no. 6 (1994): 1433–44. http://dx.doi.org/10.1083/jcb.126.6.1433.

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Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization and stability, the control of cell shape, and various motile processes. To ascertain the function of high affinity actin-membrane associations, we have disrupted by homologous recombination the gene encoding ponticulin, the major high affinity actin-membrane link in Dictyostelium discoideum amoebae. Cells lacking detectable amounts of ponticulin message and protein also are deficient in high affinity actin-membrane binding by several criteria. First, only 10-13% as much endo

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Yuzlenko, Olga, and Themis Lazaridis. "Membrane protein native state discrimination by implicit membrane models." Journal of Computational Chemistry 34, no. 9 (2012): 731–38. http://dx.doi.org/10.1002/jcc.23189.

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20

Quinones, B., K. Riento, V. M. Olkkonen, S. Hardy, and M. K. Bennett. "Syntaxin 2 splice variants exhibit differential expression patterns, biochemical properties and subcellular localizations." Journal of Cell Science 112, no. 23 (1999): 4291–304. http://dx.doi.org/10.1242/jcs.112.23.4291.

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The syntaxins are a large protein family implicated in the targeting and fusion of intracellular transport vesicles. A subset of proteins of this family are the four syntaxin 2 splice variants, syntaxins 2A (2), 2B (2′), 2C (2″) and 2D. Each syntaxin 2 variant contains an identical, or nearly identical, amino-terminal cytoplasmic domain followed by a distinct hydrophobic (syntaxins 2A and 2B) or hydrophilic (syntaxins 2C and 2D) carboxyl-terminal domain. To investigate whether the difference among the syntaxin 2 variants is functionally important, we have examined comparatively their RNA trans

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21

Feher, J. J., and G. D. Ford. "A simple student laboratory on osmotic flow, osmotic pressure, and the reflection coefficient." Advances in Physiology Education 268, no. 6 (1995): S10. http://dx.doi.org/10.1152/advances.1995.268.6.s10.

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Osmosis is usually taught from the point of view of the osmotic pressure developed when solutions of different concentrations of solute are separated by an ideal semipermeable membrane. The osmotic pressure is defined at equilibrium when there is no net flow, and it takes some time to reach this equilibrium. Although the osmotic pressure is certainly important, teaching only this point of view implicitly diminishes the importance of osmotic flow, which begins almost instantaneously across a membrane. A device was constructed with which students could measure the flow across a model membrane (d

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Angelova, Plamena R., Minee L. Choi, Alexey V. Berezhnov, et al. "Alpha synuclein aggregation drives ferroptosis: an interplay of iron, calcium and lipid peroxidation." Cell Death & Differentiation 27, no. 10 (2020): 2781–96. http://dx.doi.org/10.1038/s41418-020-0542-z.

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Abstract Protein aggregation and abnormal lipid homeostasis are both implicated in neurodegeneration through unknown mechanisms. Here we demonstrate that aggregate-membrane interaction is critical to induce a form of cell death called ferroptosis. Importantly, the aggregate-membrane interaction that drives ferroptosis depends both on the conformational structure of the aggregate, as well as the oxidation state of the lipid membrane. We generated human stem cell-derived models of synucleinopathy, characterized by the intracellular formation of α-synuclein aggregates that bind to membranes. In h

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Krausser, Johannes, Tuomas P. J. Knowles, and Anđela Šarić. "Physical mechanisms of amyloid nucleation on fluid membranes." Proceedings of the National Academy of Sciences 117, no. 52 (2020): 33090–98. http://dx.doi.org/10.1073/pnas.2007694117.

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Biological membranes can dramatically accelerate the aggregation of normally soluble protein molecules into amyloid fibrils and alter the fibril morphologies, yet the molecular mechanisms through which this accelerated nucleation takes place are not yet understood. Here, we develop a coarse-grained model to systematically explore the effect that the structural properties of the lipid membrane and the nature of protein–membrane interactions have on the nucleation rates of amyloid fibrils. We identify two physically distinct nucleation pathways—protein-rich and lipid-rich—and quantify how the me

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Ríos-Medina, Yolanda, Pedro Rico-Chávez, Ivette Martínez-Vieyra, et al. "Altered Plasma Membrane Lipid Composition in Hypertensive Neutrophils Impacts Epithelial Sodium Channel (ENaC) Endocytosis." International Journal of Molecular Sciences 25, no. 9 (2024): 4939. http://dx.doi.org/10.3390/ijms25094939.

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Biological membranes are composed of a lipid bilayer with embedded proteins, including ion channels like the epithelial sodium channel (ENaC), which are critical for sodium homeostasis and implicated in arterial hypertension (HTN). Changes in the lipid composition of the plasma membrane can significantly impact cellular processes related to physiological functions. We hypothesized that the observed overexpression of ENaC in neutrophils from HTN patients might result from alterations in the structuring domains within the plasma membrane, disrupting the endocytic processes responsible for ENaC r

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Schulz, Timothy A., Mal-Gi Choi, Sumana Raychaudhuri, et al. "Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues." Journal of Cell Biology 187, no. 6 (2009): 889–903. http://dx.doi.org/10.1083/jcb.200905007.

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Sterols are transferred between cellular membranes by vesicular and poorly understood nonvesicular pathways. Oxysterol-binding protein–related proteins (ORPs) have been implicated in sterol sensing and nonvesicular transport. In this study, we show that yeast ORPs use a novel mechanism that allows regulated sterol transfer between closely apposed membranes, such as organelle contact sites. We find that the core lipid-binding domain found in all ORPs can simultaneously bind two membranes. Using Osh4p/Kes1p as a representative ORP, we show that ORPs have at least two membrane-binding surfaces; o

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Jovanovic, Olivera A., Fraser D. Brown, and Julie G. Donaldson. "An Effector Domain Mutant of Arf6 Implicates Phospholipase D in Endosomal Membrane Recycling." Molecular Biology of the Cell 17, no. 1 (2006): 327–35. http://dx.doi.org/10.1091/mbc.e05-06-0523.

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In this study, we investigated the role of phospholipase D (PLD) in mediating Arf6 function in cells. Expression of Arf6 mutants that are defective in activating PLD, Arf6N48R and Arf6N48I, inhibited membrane recycling to the plasma membrane (PM), resulting in an accumulation of tubular endosomal membranes. Additionally, unlike wild-type Arf6, neither Arf6 mutant could generate protrusions or recruit the Arf6 GTPase activating protein (GAP) ACAP1 onto the endosome in the presence of aluminum fluoride. Remarkably, all of these phenotypes, including accumulated tubular endosomes, blocked recycli

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Ilangumaran, Subburaj, Stephan Arni, Gerhild van Echten-Deckert, Bettina Borisch, and Daniel C. Hoessli. "Microdomain-dependent Regulation of Lck and Fyn Protein-Tyrosine Kinases in T Lymphocyte Plasma Membranes." Molecular Biology of the Cell 10, no. 4 (1999): 891–905. http://dx.doi.org/10.1091/mbc.10.4.891.

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Src family protein-tyrosine kinases are implicated in signaling via glycosylphosphatidylinositol (GPI)-anchored receptors. Both kinds of molecules reside in opposite leaflets of the same sphingolipid-enriched microdomains in the lymphocyte plasma membrane without making direct contact. Under detergent-free conditions, we isolated a GPI-enriched plasma membrane fraction, also containing transmembrane proteins, selectively associated with sphingolipid microdomains. Nonionic detergents released the transmembrane proteins, yielding core sphingolipid microdomains, limited amounts of which could als

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Bandorowicz-Pikuła, J., and S. Pikuła. "Annexins and ATP in membrane traffic: a comparison with membrane fusion machinery." Acta Biochimica Polonica 45, no. 3 (1998): 721–33. http://dx.doi.org/10.18388/abp.1998_4211.

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Annexins, calcium- and membrane-binding multifunctional proteins, have been implicated in N-ethylmaleimide (NEM)-independent fusion of vesicular structures involved in membrane traffic. This view is based on intracellular localization of annexins, which are frequently associated with endosomes, chromaffin granules, caveolae, clathrin-coated pits, and other membrane compartments, engaged in endo- and exocytosis. Moreover, annexins were found to modulate budding and aggregation of vesicle membranes, to interact with cytoskeletal proteins, and, upon binding to membranes, to change the structure o

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Zhang, Guangzhi, and Hélène Sanfaçon. "Characterization of Membrane Association Domains within the Tomato Ringspot Nepovirus X2 Protein, an Endoplasmic Reticulum-Targeted Polytopic MembraneProtein." Journal of Virology 80, no. 21 (2006): 10847–57. http://dx.doi.org/10.1128/jvi.00789-06.

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ABSTRACT Replication of nepoviruses (family Comoviridae) occurs in association with endoplasmic reticulum (ER)-derived membranes. We have previously shown that the putative nucleoside triphosphate-binding protein (NTB) of Tomato ringspot nepovirus is an integral membrane protein with two ER-targeting sequences and have suggested that it anchors the viral replication complex (VRC) to the membranes. A second highly hydrophobic protein domain (X2) is located immediately upstream of the NTB domain in the RNA1-encoded polyprotein. X2 shares conserved sequence motifs with the comovirus 32-kDa protei

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Hinshaw, J. E., and K. R. Miller. "Localization of light-harvesting complex II to the occluded surfaces of photosynthetic membranes." Journal of Cell Biology 109, no. 4 (1989): 1725–31. http://dx.doi.org/10.1083/jcb.109.4.1725.

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The photosynthetic membranes of green plants are organized into stacked regions interconnected by nonstacked regions that have been shown to be biochemically and structurally distinct. Because the stacking process occludes the surfaces of appressed membranes, it has been impossible to conduct structural or biochemical studies of the outer surfaces of the photosynthetic membrane in regions of membrane stacking. Although stacking is mediated at this surface, it has not been possible to determine whether membrane components implicated in the stacking process, including a major light-harvesting co

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Beraki, Tsebaot, Xiaoyu Hu, Malgorzata Broncel, et al. "Divergent kinase regulates membrane ultrastructure of theToxoplasmaparasitophorous vacuole." Proceedings of the National Academy of Sciences 116, no. 13 (2019): 6361–70. http://dx.doi.org/10.1073/pnas.1816161116.

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Apicomplexan parasites replicate within a protective organelle, called the parasitophorous vacuole (PV). TheToxoplasma gondiiPV is filled with a network of tubulated membranes, which are thought to facilitate trafficking of effectors and nutrients. Despite being critical to parasite virulence, there is scant mechanistic understanding of the network’s functions. Here, we identify the parasite-secreted kinase WNG1 (With-No-Gly-loop) as a critical regulator of tubular membrane biogenesis. WNG1 family members adopt an atypical protein kinase fold lacking the glycine rich ATP-binding loop that is r

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Fan, Weiliang, Jia Guo, Beichen Gao, et al. "Flotillin-mediated endocytosis and ALIX–syntenin-1–mediated exocytosis protect the cell membrane from damage caused by necroptosis." Science Signaling 12, no. 583 (2019): eaaw3423. http://dx.doi.org/10.1126/scisignal.aaw3423.

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Necroptosis is a form of regulated necrosis that is implicated in various human diseases including Alzheimer’s disease. Necroptosis requires the translocation of the pseudokinase MLKL from the cytosol to the plasma membrane after its phosphorylation by the kinase RIPK3. Using protein cross-linking followed by affinity purification, we detected the lipid raft–associated proteins flotillin-1 and flotillin-2 and the ESCRT-associated proteins ALIX and syntenin-1 in membrane-localized MLKL immunoprecipitates. Phosphorylated MLKL was removed from membranes through either flotillin-mediated endocytos

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Lanrezac, André, Benoist Laurent, Hubert Santuz, Nicolas Férey, and Marc Baaden. "Fast and Interactive Positioning of Proteins within Membranes." Algorithms 15, no. 11 (2022): 415. http://dx.doi.org/10.3390/a15110415.

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(1) Background: We developed an algorithm to perform interactive molecular simulations (IMS) of protein alignment in membranes, allowing on-the-fly monitoring and manipulation of such molecular systems at various scales. (2) Methods: UnityMol, an advanced molecular visualization software; MDDriver, a socket for data communication; and BioSpring, a Spring network simulation engine, were extended to perform IMS. These components are designed to easily communicate with each other, adapt to other molecular simulation software, and provide a development framework for adding new interaction models t

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Schulman, G., R. Hakim, R. Arias, M. Silverberg, A. P. Kaplan, and L. Arbeit. "Bradykinin generation by dialysis membranes: possible role in anaphylactic reaction." Journal of the American Society of Nephrology 3, no. 9 (1993): 1563–69. http://dx.doi.org/10.1681/asn.v391563.

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Several recent reports have described a high incidence of anaphylactic reactions in patients being dialyzed with high-flux membranes while simultaneously using angiotensin-converting enzyme inhibitors. Many of these reports implicate polyacrylonitrile (PAN) as the membrane commonly involved in these reactions. To elucidate potential mechanisms of these anaphylactic reactions, whether dialysis membranes can activate the Hageman factor-dependent (contact) pathways as assessed by the in vitro generation of activated Hageman factor (Hfa), as well as the formation of kallikrein and subsequent brady

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Sharma, Mahak, Sai Srinivas Panapakkam Giridharan, Juliati Rahajeng, Naava Naslavsky, and Steve Caplan. "MICAL-L1 Links EHD1 to Tubular Recycling Endosomes and Regulates Receptor Recycling." Molecular Biology of the Cell 20, no. 24 (2009): 5181–94. http://dx.doi.org/10.1091/mbc.e09-06-0535.

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Endocytic recycling of receptors and lipids occurs via a complex network of tubular and vesicular membranes. EHD1 is a key regulator of endocytosis and associates with tubular membranes to facilitate recycling. Although EHD proteins tubulate membranes in vitro, EHD1 primarily associates with preexisting tubules in vivo. How EHD1 is recruited to these tubular endosomes remains unclear. We have determined that the Rab8-interacting protein, MICAL-L1, associates with EHD1, with both proteins colocalizing to long tubular membranes, in vitro and in live cells. MICAL-L1 is a largely uncharacterized m

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Klotz, K. N., K. L. Krotec, J. Gripentrog, and A. J. Jesaitis. "Regulatory interaction of N-formyl peptide chemoattractant receptors with the membrane skeleton in human neutrophils." Journal of Immunology 152, no. 2 (1994): 801–10. http://dx.doi.org/10.4049/jimmunol.152.2.801.

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Abstract The cytoskeleton and/or membrane skeleton has been implicated in the regulation of N-formyl peptide receptors. The coupling of these chemotactic receptors to the membrane skeleton was investigated in plasma membranes from unstimulated and desensitized human neutrophils using the photoreactive agonist N-formyl-met-leu-phe-lys-N epsilon-[125I]2(p-azidosalicylamido)ethyl-1,3'- dithiopropionate (fMLFK-[125I]ASD). When membranes of unstimulated cells were solubilized in Triton-X 100, a detergent that does not disrupt actin filaments, only 50% of the photoaffinity-labeled receptors were sol

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Levi, Valeria, Ana M. Villamil Giraldo, Pablo R. Castello, Juan P. F. C. Rossi, and F. Luis González Flecha. "Effects of phosphatidylethanolamine glycation on lipid–protein interactions and membrane protein thermal stability." Biochemical Journal 416, no. 1 (2008): 145–52. http://dx.doi.org/10.1042/bj20080618.

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Non-enzymatic glycation of biomolecules has been implicated in the pathophysiology of aging and diabetes. Among the potential targets for glycation are biological membranes, characterized by a complex organization of lipids and proteins interacting and forming domains of different size and stability. In the present study, we analyse the effects of glycation on the interactions between membrane proteins and lipids. The phospholipid affinity for the transmembrane surface of the PMCA (plasma-membrane Ca2+-ATPase) was determined after incubating the protein or the phospholipids with glucose. Resul

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Ahn, Anna, Don L. Gibbons, and Margaret Kielian. "The Fusion Peptide of Semliki Forest Virus Associates with Sterol-Rich Membrane Domains." Journal of Virology 76, no. 7 (2002): 3267–75. http://dx.doi.org/10.1128/jvi.76.7.3267-3275.2002.

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ABSTRACT Semliki Forest virus (SFV) is an enveloped alphavirus whose membrane fusion is triggered by low pH and promoted by cholesterol and sphingolipid in the target membrane. Fusion is mediated by E1, a viral membrane protein containing the putative fusion peptide. Virus mutant studies indicate that SFV's cholesterol dependence is controlled by regions of E1 outside of the fusion peptide. Both E1 and E1*, a soluble ectodomain form of E1, interact with membranes in a reaction dependent on low pH, cholesterol, and sphingolipid and form highly stable homotrimers. Here we have used detergent ext

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Wall, J., F. Ayoub, and P. O'Shea. "Interactions of macromolecules with the mammalian cell surface." Journal of Cell Science 108, no. 7 (1995): 2673–82. http://dx.doi.org/10.1242/jcs.108.7.2673.

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The characterisation of fluoresceinphosphatidylethanolamine (FPE) as a real-time indicator of the electrostatic nature of the cell membrane surface is described. The conditions appropriate for the labelling of cell membranes and the implementation of FPE as a tool to monitor the interactions of various proteins and peptides with membranes are outlined. Some complications attributed to the erythrocyte glycocalyx are examined. In addition it is shown using neuraminidase as an example, that some types of enzyme-catalysed reactions on the cell surface may be monitored in real time. It is also show

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Pavlov, Evgeny V., Muriel Priault, Dawn Pietkiewicz, et al. "A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast." Journal of Cell Biology 155, no. 5 (2001): 725–32. http://dx.doi.org/10.1083/jcb.200107057.

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During apoptosis, proapoptotic factors are released from mitochondria by as yet undefined mechanisms. Patch-clamping of mitochondria and proteoliposomes formed from mitochondrial outer membranes of mammalian (FL5.12) cells has uncovered a novel ion channel whose activity correlates with onset of apoptosis. The pore diameter inferred from the largest conductance state of this channel is ∼4 nm, sufficient to allow diffusion of cytochrome c and even larger proteins. The activity of the channel is affected by Bcl-2 family proteins in a manner consistent with their pro- or antiapoptotic properties.

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Ngo, Mike H., Terry R. Colbourne, and Neale D. Ridgway. "Functional implications of sterol transport by the oxysterol-binding protein gene family." Biochemical Journal 429, no. 1 (2010): 13–24. http://dx.doi.org/10.1042/bj20100263.

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Cholesterol and its numerous oxygenated derivatives (oxysterols) profoundly affect the biophysical properties of membranes, and positively and negatively regulate sterol homoeostasis through interaction with effector proteins. As the bulk of cellular sterols are segregated from the sensory machinery that controls homoeostatic responses, an important regulatory step involves sterol transport or signalling between membrane compartments. Evidence for rapid, energy-independent transport between organelles has implicated transport proteins, such as the eukaryotic family of OSBP (oxysterol-binding p

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Liu, Hualan, W. Keith Ray, Richard F. Helm, David L. Popham, and Stephen B. Melville. "Analysis of the Spore Membrane Proteome in Clostridium perfringens Implicates Cyanophycin in Spore Assembly." Journal of Bacteriology 198, no. 12 (2016): 1773–82. http://dx.doi.org/10.1128/jb.00212-16.

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ABSTRACTHeat-resistant endospore formation plays an important role inClostridium perfringens-associated foodborne illnesses. The spores allow the bacterium to survive heating during normal cooking processes, followed by germination and outgrowth of the bacterium in contaminated foods. To identify proteins associated with germination and other spore functions, a comparative spore membrane proteome analysis of dormant and germinated spores ofC. perfringensstrain SM101 was performed by using gel-based protein separation and liquid chromatography coupled with matrix-assisted laser desorption ioniz

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Kweon, Youngseok, Anca Rothe, Elizabeth Conibear, and Tom H. Stevens. "Ykt6p Is a Multifunctional Yeast R-SNARE That Is Required for Multiple Membrane Transport Pathways to the Vacuole." Molecular Biology of the Cell 14, no. 5 (2003): 1868–81. http://dx.doi.org/10.1091/mbc.e02-10-0687.

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Intracellular membrane fusion requires that membrane-bound soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins on both vesicle and target membranes form a highly specific complex necessary to bring the membranes close in space. Ykt6p is a yeast R-SNARE protein that has been implicated in retrograde transport to the cis-Golgi compartment. Ykt6p has been also been found to fractionate with vacuole membranes and participate in a vacuolar SNARE complex in homotypic vacuole fusion. To investigate the role of Ykt6p in membrane traffic to the vacuole we generated te

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Sorek, Nadav, Limor Poraty, Hasana Sternberg, Enat Bar, Efraim Lewinsohn, and Shaul Yalovsky. "Activation Status-Coupled Transient S Acylation Determines MembranePartitioning of a Plant Rho-Related GTPase." Molecular and Cellular Biology 27, no. 6 (2007): 2144–54. http://dx.doi.org/10.1128/mcb.02347-06.

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ABSTRACT ROPs or RACs are plant Rho-related GTPases implicated in the regulation of a multitude of signaling pathways that function at the plasma membrane by virtue of posttranslational lipid modifications. The relationship between ROP activation status and membrane localization has not been established. Here we demonstrate that endogenous ROPs, as well as a transgenic His6-green fluorescent protein (GFP)-AtROP6 fusion protein, were partitioned between Triton X-100-soluble and -insoluble membranes. In contrast, an activated His6-GFP-Atrop6CA mutant protein accumulated exclusively in detergent-

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Ponmalar, Ilanila I., Ramesh Cheerla, K. Ganapathy Ayappa, and Jaydeep K. Basu. "Correlated protein conformational states and membrane dynamics during attack by pore-forming toxins." Proceedings of the National Academy of Sciences 116, no. 26 (2019): 12839–44. http://dx.doi.org/10.1073/pnas.1821897116.

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Pore-forming toxins (PFTs) are a class of proteins implicated in a wide range of virulent bacterial infections and diseases. These toxins bind to target membranes and subsequently oligomerize to form functional pores that eventually lead to cell lysis. While the protein undergoes large conformational changes on the bilayer, the connection between intermediate oligomeric states and lipid reorganization during pore formation is largely unexplored. Cholesterol-dependent cytolysins (CDCs) are a subclass of PFTs widely implicated in food poisoning and other related infections. Using a prototypical

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Mayer, A., I. E. Ivanov, D. Gravotta, M. Adesnik, and D. D. Sabatini. "Cell-free reconstitution of the transport of viral glycoproteins from the TGN to the basolateral plasma membrane of MDCK cells." Journal of Cell Science 109, no. 7 (1996): 1667–76. http://dx.doi.org/10.1242/jcs.109.7.1667.

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An in vitro system to study the transport of plasma membrane proteins from the TGN to the basolateral plasma membrane of polarized MDCK cells has been developed in which purified cell fractions are combined and transport between them is studied under controlled conditions. In this system, a donor Golgi fraction derived from VSV or influenza virus-infected MDCK cells, in which 35S-labeled viral glycoproteins were allowed to accumulate in the TGN during a low temperature block, is incubated with purified immobilized basolateral plasma membranes that have their cytoplasmic face exposed and are ob

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Georgatos, S. D., D. C. Weaver, and V. T. Marchesi. "Site specificity in vimentin-membrane interactions: intermediate filament subunits associate with the plasma membrane via their head domains." Journal of Cell Biology 100, no. 6 (1985): 1962–67. http://dx.doi.org/10.1083/jcb.100.6.1962.

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Fragments of vimentin, generated by chemical or enzymatic cleavages, were analyzed for their capacity to bind to human inverted erythrocyte membrane vesicles. Only peptides comprising the amino-terminal head domain of vimentin molecules were competent in associating with the membranes. In vitro studies also demonstrated that isolated ankyrin (the major vimentin acceptor site on the membrane) binds to an oligomeric species of vimentin and prevents the formation of characteristic 10-nm filaments. These data, taken together with the observation that the NH2-terminal end of vimentin is implicated

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Bkaily, Ghassan, Yanick Simon, Ashley Jazzar, Houssein Najibeddine, Alexandre Normand, and Danielle Jacques. "High Na+ Salt Diet and Remodeling of Vascular Smooth Muscle and Endothelial Cells." Biomedicines 9, no. 8 (2021): 883. http://dx.doi.org/10.3390/biomedicines9080883.

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Our knowledge on essential hypertension is vast, and its treatment is well known. Not all hypertensives are salt-sensitive. The available evidence suggests that even normotensive individuals are at high cardiovascular risk and lower survival rate, as blood pressure eventually rises later in life with a high salt diet. In addition, little is known about high sodium (Na+) salt diet-sensitive hypertension. There is no doubt that direct and indirect Na+ transporters, such as the Na/Ca exchanger and the Na/H exchanger, and the Na/K pump could be implicated in the development of high salt-induced hy

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Deretic, D., L. A. Huber, N. Ransom, M. Mancini, K. Simons, and D. S. Papermaster. "rab8 in retinal photoreceptors may participate in rhodopsin transport and in rod outer segment disk morphogenesis." Journal of Cell Science 108, no. 1 (1995): 215–24. http://dx.doi.org/10.1242/jcs.108.1.215.

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Small GTP-binding protein rab8 regulates transport from the TGN to the basolateral plasma membrane in epithelial cells and to the dendritic plasma membrane in cultured hippocampal neurons. In our approach to identify proteins involved in rhodopsin transport and sorting in retinal photoreceptors, we have found, using [32P]GTP overlays of 2D gel blots, that six small GTP-binding proteins are tightly bound to the post-Golgi membranes immunoisolated with a mAb to the cytoplasmic domain of frog rhodopsin. We report here that one of these proteins is rab8. About 50% of photoreceptor rab8 is membrane

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Siess, Katharina M., and Thomas A. Leonard. "Lipid-dependent Akt-ivity: where, when, and how." Biochemical Society Transactions 47, no. 3 (2019): 897–908. http://dx.doi.org/10.1042/bst20190013.

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Abstract Akt is an essential protein kinase activated downstream of phosphoinositide 3-kinase and frequently hyperactivated in cancer. Canonically, Akt is activated by phosphoinositide-dependent kinase 1 and mechanistic target of rapamycin complex 2, which phosphorylate it on two regulatory residues in its kinase domain upon targeting of Akt to the plasma membrane by PI(3,4,5)P3. Recent evidence, however, has shown that, in addition to phosphorylation, Akt activity is allosterically coupled to the engagement of PI(3,4,5)P3 or PI(3,4)P2 in cellular membranes. Furthermore, the active membrane-bo

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