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Journal articles on the topic 'Merz'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Naguib, Martha M., Ahmed O. El-Gendy, and Ahmed S. Khairalla. "Microbial Diversity of Mer Operon Genes and Their Potential Rules in Mercury Bioremediation and Resistance." Open Biotechnology Journal 12, no. 1 (April 30, 2018): 56–77. http://dx.doi.org/10.2174/1874070701812010056.

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Background:Mercury is a toxic metal that is present in small amounts in the environment, but its level is rising steadily, due to different human activities, such as industrialization. It can reach humans through the food chain, amalgam fillings, and other sources, causing different neurological disorders, memory loss, vision impairment, and may even lead to death; making its detoxification an urgent task.Methods:Various physical and chemical mercury remediation techniques are available, which generally aim at: (i) reducing its mobility or solubility; (ii) causing its vaporization or condensation; (iii) its separation from contaminated soils. Biological remediation techniques, commonly known as bioremediation, are also another possible alternative, which is considered as cheaper than the conventional means and can be accomplished using either (i) organisms harboring themeroperon genes (merB,merA,merR,merP,merT,merD,merF,merC,merE,merHandmerG), or (ii) plants expressing metal-binding proteins. Recently, differentmerdeterminants have been genetically engineered into several organisms, including bacteria and plants, to aid in detoxification of both ionic and organic forms of mercury.Results:Bacteria that are resistant to mercury compounds have at least a mercuric reductase enzyme (MerA) that reduces Hg+2to volatile Hg0, a membrane-bound protein (MerT) for Hg+2uptake and an additional enzyme, MerB, that degrades organomercurials by protonolysis. Presence of bothmerA andmerB genes confer broad-spectrum mercury resistance. However,merA alone confers narrow spectrum inorganic mercury resistance.Conclusion:To conclude, this review discusses the importance of mercury-resistance genes in mercury bioremediation. Functional analysis ofmeroperon genes and the recent advances in genetic engineering techniques could provide the most environmental friendly, safe, effective and fantastic solution to overcome mercuric toxicity.

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2

Nagy, Božidar. "Vladimir Nazor u recepciji studenta Ivana Merza." Obnovljeni život 73., no. 1. (May 23, 2018): 39–52. http://dx.doi.org/10.31337/oz.73.1.3.

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Dvadesetogodišnji student Ivan Merz otkrio je veličinu Vladimira Nazora kao pjesnika i književnika za vrijeme Prvog svjetskog rata 1916. na počeku svojeg vojništva. Bilo je to u Slovenskoj Bistrici, kamo je bio poslan na časnički tečaj, gdje je susreo Nazorova prijatelja i suradnika Josipa Ribarića, jezikoslovca. Ribarić ga je potaknuo i oduševio za Nazora i njegova književna djela, koja je Merz čitao u slobodno vrijeme. Svoje dojmove o Nazorovim djelima Merz najprije priopćuje u pismima svojemu profesoru Ljubomiru Marakoviću. Istodobno u svojem ratnom dnevniku donosi prikaze Nazorovih djela koja je čitao te daje o njima svoje ocjene, mišljenje i pohvale. U ovom članku donose se uz uvodni komentar najvažniji citati iz pisama i dnevničkih zapisa Ivana Merza u kojima govori o Nazoru. Da se dobije kompletnija slika o Merzu, kao i o Nazoru, Merzovim tekstovima pridodali smo i navode o Nazoru iz Marakovićevih pisama upućenih Ivanu Merzu. Merz je bio oduševljen otkrićem Nazorove poezije i smatrao je Nazora kao pjesnika „hrvatskim velikanom“. Posebno je dojmljiva Merzova izjava da su „Hrvati slijepi što ne vide kako velikog pjesnika imaju, kao što je Nazor“. Smatra da će tek nakon njegove smrti „vidjeti da je bio velikan“.

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3

Lenman, Sören, Henry See, and Michael Century. "Merz." ACM SIGWEB Newsletter 5, no. 2 (June 1996): 10. http://dx.doi.org/10.1145/231738.232579.

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4

le Vert, Rick. "Thomas Merz." Information Design Journal 9, no. 1 (January 1, 1998): 67–69. http://dx.doi.org/10.1075/idj.9.1.14ver.

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5

Ravel, Jacques, Jocelyne DiRuggiero, Frank T. Robb, and Russell T. Hill. "Cloning and Sequence Analysis of the Mercury Resistance Operon of Streptomyces sp. Strain CHR28 Reveals a Novel Putative Second Regulatory Gene." Journal of Bacteriology 182, no. 8 (April 15, 2000): 2345–49. http://dx.doi.org/10.1128/jb.182.8.2345-2349.2000.

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ABSTRACT A DNA library of pRJ28, a large linear plasmid encoding mercury resistance, was constructed, and the mercury resistance genes were cloned. The 5,921-bp sequence was analyzed and showed a high degree of similarity to the Streptomyces lividans 1326 mercury resistance operon. Genes merR, merT,merP, and orfIV were found in a similar order and in a single transcription unit. merA andmerB were found to be transcribed in the opposite direction to genes merR, merT, merP, andorfIV, as in S. lividans 1326. A novel putative regulatory gene, orfX, was found 22 bp downstream ofmerA. orfX encodes a 137-amino acid protein with a potential helix-turn-helix motif in the N-terminal domain, characteristic of the MerR family of transcriptional regulators. Transcriptional studies showed that orfX is cotranscribed with merA and merB. It is hypothesized thatorfX plays a role in the regulation of the mercury resistance operon, probably by binding at the MerR operator site.

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6

ALPER AKÇAY, Ayça. "DIŞAVURUMDAN YAPISALCILIĞA MERZ YAPISI." Journal of Social Sciences 3, no. 6 (January 1, 2016): 185. http://dx.doi.org/10.16990/sobider.153.

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7

Raposo, Pedro M. P. "Merz Telescopes in Italy." Journal for the History of Astronomy 49, no. 2 (May 2018): 265–66. http://dx.doi.org/10.1177/0021828618773232.

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8

Schwitters, Kurt, and Harriet Watts. "The Onion Merz Poem 8." Assemblage, no. 1 (October 1986): 84. http://dx.doi.org/10.2307/3171056.

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9

Patsko, Valerii, and Varvara Turova. "Antony Merz and His Works." Dynamic Games and Applications 10, no. 1 (July 9, 2019): 157–82. http://dx.doi.org/10.1007/s13235-019-00318-y.

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10

Knoppers, Bartha Maria. "Reply to Merz et al." American Journal of Human Genetics 63, no. 3 (September 1998): 900. http://dx.doi.org/10.1086/302030.

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11

Lindt, Grazia. "Klaus Merz: Der Argentinier. Novelle." Zeitschrift für interkulturelle Germanistik 1, no. 2 (December 1, 2010): 143–44. http://dx.doi.org/10.14361/zig.2010.0213.

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12

Chinnici, Ileana, and Paolo Brenni. "The Palermo Merz Equatorial Telescope." Nuncius 30, no. 1 (2015): 228–79. http://dx.doi.org/10.1163/18253911-03001009.

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A manuscript by Georg and Sigmund Merz dated 1862 and containing instructions for assembling the equatorial telescope acquired by the Palermo Observatory is conserved in the archives of the Museo Astronomico e Copernicano in Rome. It is a rare document that reveals “tricks of the trade” and technical knowledge not usually included in textbooks or treatises. It was sent to the Palermo Observatory as an aid to the installation of the telescope, which made a signal contribution to the development of solar physics in Italy in the 19th century. Based on the study of unpublished sources (consisting of texts and drawings), the history of the instrument has been retraced. This paper presents a detailed description of the Merz manuscript (including a complete transcript) and some technical drawings recently discovered in the archives of the Palermo Observatory.

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13

Häßler, Miriam. "Moscow Merz and Russian Rhythm." Experiment 23, no. 1 (October 11, 2017): 117–26. http://dx.doi.org/10.1163/2211730x-12341305.

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Abstract The Erste Russische Kunstausstellung [First Russian Art Exhibition] of 1922 was a remarkable event not only for Berlin’s art lovers at that time, but also for the history of twentieth century art. Held at Galerie van Diemen, the show gave a comprehensive overview of Russia’s artistic achievements from late Tsardom to the Russian Civil War. Of all styles in the exhibition, the non-objective art movements of suprematism and constructivism provoked the greatest sensation among the visitors, many of whom were Western artists. Relating Russia’s variations of non-objectivity with their—assumed—political notions, Western modernists reacted in various ways. This article aims at tracking the long-lasting vestiges of the Erste Russische Kunstausstellung in the personal and artistic developments of two key-figures of Germany’s modern art scene: Kurt Schwitters and Hans Richter. While the role of El Lissitzky, who designed the catalogue’s cover, has already been canonized, this article wants to highlight lesser-regarded aspects.

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14

Schwarz, Dieter. "The Irony of Marisa Merz." October 124 (May 2008): 157–68. http://dx.doi.org/10.1162/octo.2008.124.1.157.

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15

Verhoeven, Paul. "Contra Merz. Een repliek op A. Merz’ artikel ‘Ein Jesus ohne “spin” und “special effects” ’." NTT Journal for Theology and the Study of Religion 63, no. 3 (August 18, 2009): 205–20. http://dx.doi.org/10.5117/ntt2009.63.205.verh.

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This is a reply to a paper by A. Merz, Ein Jesus ohne ‘spin’ und ‘special effects’, in which she states that my 2008 book Jezus van Nazaret is a methodological failure. I want to show that Merz reaches this conclusion by unfair methods. First: she characterizes my argumentation in a general way as ‘implausible’ but never mentions the content of my arguments, let alone discusses them. Second: whenever I quote established theologians whose ideas I used, Merz systematically avoids mentioning their names, instead proceeds as if these ideas are mine ‐ which gives her the academic freedom to belittle them. Third: she paraphrases my text in mocking hyperboles, thereby misleading the reader. Merz’s goal is apologetic: she tries to undermine the image of Jesus I presented in my book.

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16

Sone, Yuka, Ryosuke Nakamura, Hidemitsu Pan-Hou, Tomoo Itoh, and Masako Kiyono. "Role of MerC, MerE, MerF, MerT, and/or MerP in Resistance to Mercurials and the Transport of Mercurials in Escherichia coli." Biological and Pharmaceutical Bulletin 36, no. 11 (2013): 1835–41. http://dx.doi.org/10.1248/bpb.b13-00554.

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17

Ritchie, J. M., and Gwendolen Webster. "Kurt Merz Schwitters: A Biographical Study." Modern Language Review 95, no. 2 (April 2000): 570. http://dx.doi.org/10.2307/3736237.

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18

Matić, Zdravko, and Krešimir Lagetar. "Ivan Merz, student na Vojnoj akademiji u Bečkom Novom Mjestu 1914. godine." Obnovljeni život 71, no. 3 (November 9, 2016): 321–33. http://dx.doi.org/10.31337/oz.71.3.3.

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Autori na temelju arhivske građe, novinskih članaka i recentne literature istražuju boravak Ivana Merza na Vojnoj akademiji u Bečkom Novom Mjestu. Iako akademija nije bio njegov izbor, nakon mature po želji roditelja mladi Merz se upisao na Vojnu akademiju u Bečkom Novom Mjestu. Bilo je to teško razdoblje u njegovu životu, kako i sam priznaje najprije u svojem Dnevniku a kasnije se povjerio i svojemu gimnazijskomu profesoru Ljubomiru Marakoviću. Grubost i nemoral na Vojnoj akademiji bili su svakodnevica, a uz činjenicu da će čitav život morati raditi posao koji nije želio bili su mu posve oprečni. Njegova želja i afinitet bili su da studira književnost i postane profesor, te je nakon tri mjeseca, uz suglasnost roditelja, napustio Vojnu akademiju, na kojoj je boravio od 12. rujna do 22. prosinca 1914. godine. Ivan Merz je odlukom Pape Ivana Pavla II. 22. lipnja 2003. u Banjoj Luci proglašen blaženikom Katoličke Crkve.

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19

Yu, Chenxu, and Sundaram Gunasekaran. "Correlation of Dynamic and Steady Flow Viscosities of Food Materials." Applied Rheology 11, no. 3 (June 1, 2001): 134–40. http://dx.doi.org/10.1515/arh-2001-0008.

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AbstractEight commercial foods representing a wide range of viscosities (i.e. honey, condensed milk, mayonnaise, tomato ketchup, cream cheese, yogurt, process and Mozzarella cheeses) were investigated. Their steady shear viscosity and dynamic complex viscosity were determined by rheological measurements at two temperatures using a Bohlin-CVO rheometer. Based on experimental data, shear rate dependence of steady flow apparent viscosity and frequency dependence of dynamic viscosity was established and compared. It was determined that for condensed milk, tomato ketchup and mayonnaise, a modified Cox-Merz relation could be established. For cream cheese, a generalized Cox-Merz relation was proposed; and for yogurt, a deviation from the Cox-Merz rule was found. For Mozzarella and process cheeses a sharp drop in steady shear viscosity was noticed between 1~10 s-1 shear rate range. The Cox-Merz rule was not applicable for these cheese samples.

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20

Dietrich, Dorothea, and Kurt Schwitters. "The Fragment Reframed: Kurt Schwitters's "Merz-Column"." Assemblage, no. 14 (April 1991): 82. http://dx.doi.org/10.2307/3171101.

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21

Hudson, Martyn. "Schwitters’s Ursonate and the Merz Barn Wall." Leonardo Music Journal 25 (December 2015): 89–92. http://dx.doi.org/10.1162/lmj_a_00942.

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This paper notes the importance of Kurt Schwitters’s Merz project to the modernist politics and poetics of exile of the 20th century. Placing the sound work of Schwitters within his full Merz project, the author assesses the relations between the Ursonate and the final Merzbau. He discusses three of these relations—collage, found objects and the structures of building materiality in language and sculpture—and presents Schwitters’s work as culminating in a vision of sound and building structures in what Brandon Taylor has called “intrusive new entities” of collage and assemblage that are themselves analogous to the “intrusive new entities” of human material itself.

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22

Richmond, C. "Lucky Hans and Other Merz Fairy Tales." Common Knowledge 16, no. 3 (September 1, 2010): 562. http://dx.doi.org/10.1215/0961754x-2010-024.

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23

Rathner, Raffael, Wolfgang Roland, Hanny Albrecht, Franz Ruemer, and Jürgen Miethlinger. "Applicability of the Cox-Merz Rule to High-Density Polyethylene Materials with Various Molecular Masses." Polymers 13, no. 8 (April 9, 2021): 1218. http://dx.doi.org/10.3390/polym13081218.

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The Cox-Merz rule is an empirical relationship that is commonly used in science and industry to determine shear viscosity on the basis of an oscillatory rheometry test. However, it does not apply to all polymer melts. Rheological data are of major importance in the design and dimensioning of polymer-processing equipment. In this work, we investigated whether the Cox-Merz rule is suitable for determining the shear-rate-dependent viscosity of several commercially available high-density polyethylene (HDPE) pipe grades with various molecular masses. We compared the results of parallel-plate oscillatory shear rheometry using the Cox-Merz empirical relation with those of high-pressure capillary and extrusion rheometry. To assess the validity of these techniques, we used the shear viscosities obtained by these methods to numerically simulate the pressure drop of a pipe head and compared the results to experimental measurements. We found that, for the HDPE grades tested, the viscosity data based on capillary pressure flow of the high molecular weight HDPE describes the pressure drop inside the pipe head significantly better than do data based on parallel-plate rheometry applying the Cox-Merz rule. For the lower molecular weight HDPE, both measurement techniques are in good accordance. Hence, we conclude that, while the Cox-Merz relationship is applicable to lower-molecular HDPE grades, it does not apply to certain HDPE grades with high molecular weight.

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24

Snow, A. "Ferranti and Merz: power transmission system design engineers." Engineering Science & Education Journal 7, no. 1 (February 1, 1998): 5–10. http://dx.doi.org/10.1049/esej:19980102.

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25

Tofield, A. "C. Noel Bairey Merz MD FACC FAHA FESC." European Heart Journal 40, no. 10 (March 6, 2019): 795–96. http://dx.doi.org/10.1093/eurheartj/ehz061.

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26

Winter, H. Henning. "Three views of viscoelasticity for Cox–Merz materials." Rheologica Acta 48, no. 3 (December 3, 2008): 241–43. http://dx.doi.org/10.1007/s00397-008-0329-5.

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27

Chae, Byeong S., Alan M. Lane, and John M. Wiest. "On the Cox-Merz rule for magnetic dispersions." Rheologica Acta 40, no. 6 (November 1, 2001): 599–602. http://dx.doi.org/10.1007/s003970100190.

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28

Peucker, Henning. "Philippe Merz: Werterfahrung und Wahrheit. Phänomenologische Ethikbegründung nach Husserl." Philosophische Rundschau 64, no. 3 (2017): 272. http://dx.doi.org/10.1628/003181517x15167161095381.

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29

Manero, O., F. Bautista, J. F. A. Soltero, and J. E. Puig. "Dynamics of worm-like micelles: the Cox–Merz rule." Journal of Non-Newtonian Fluid Mechanics 106, no. 1 (August 2002): 1–15. http://dx.doi.org/10.1016/s0377-0257(02)00082-4.

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30

Ishii, Masahiko, and Hiroshi Nakamura. "Applicability of Modified Cox-Merz Rule to Concentrated Suspensions." Journal of Non-Newtonian Fluid Mechanics 282 (August 2020): 104322. http://dx.doi.org/10.1016/j.jnnfm.2020.104322.

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31

Krylova, Katya. "Der Herr Karl by Helmut Qualtinger and Carl Merz." Journal of Austrian Studies 52, no. 4 (2019): 131–34. http://dx.doi.org/10.1353/oas.2019.0064.

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32

Moers, Stephanie. "Interview mit Philippe Merz – „Es wird zu wenig geatmet“." physiopraxis 15, no. 04 (April 21, 2017): 34–37. http://dx.doi.org/10.1055/s-0043-101659.

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33

Makela, Maria. "Making Lemonade out of Lemons: Merz and Material Poverty." Art History 42, no. 4 (August 12, 2019): 652–77. http://dx.doi.org/10.1111/1467-8365.12455.

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34

Gleissle, W., and B. Hochstein. "Validity of the Cox–Merz rule for concentrated suspensions." Journal of Rheology 47, no. 4 (July 2003): 897–910. http://dx.doi.org/10.1122/1.1574020.

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35

Collier, Helena. "Merz Aesthetics Expert Summit, Paris 2013: ‘an unforgettable event’." Journal of Aesthetic Nursing 2, no. 10 (January 2014): 506. http://dx.doi.org/10.12968/joan.2013.2.10.506.

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36

Shan, Liyan, Yiqiu Tan, and Y. Richard Kim. "Applicability of the Cox–Merz relationship for asphalt binder." Construction and Building Materials 37 (December 2012): 716–22. http://dx.doi.org/10.1016/j.conbuildmat.2012.07.020.

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37

Schluchter, Jan-René. "Jan-René Schluchter im Gespräch mit Kerstin Merz-Atalik." Ludwigsburger Beiträge zur Medienpädagogik 15 (January 1, 2012): 1–5. http://dx.doi.org/10.21240/lbzm/15/11.

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38

Korneyev, S. V., and A. Klasa. "New Records and a Revised Checklist of Fruit Flies of the Genus Tephritis (Diptera, Tephritidae) from Ukraine." Vestnik Zoologii 50, no. 3 (June 1, 2016): 219–24. http://dx.doi.org/10.1515/vzoo-2016-0025.

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Abstract Thirty-one Tephritis species are recorded from Ukraine. Six species (T. brachyura Loew, T. conyzifoliae Merz, T. heliophila Hendel, T. mutabilis Merz, T. separata Rondani, T. tanaceti Hering, and T. valida Loew) are recorded for the first time from Ukraine; two species (T. crepidis Hendel and T. leontodontis De Geer) listed before without any references to material or locality are confirmed to occur in Ukraine, and two species on the list (T. conura Loew and T. hendeliana Hering) have not been confirmed yet by any available material, but possibly present in western and southern regions.

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39

Kokovina, Yu, E. Yu Pavlova, and E. A. Antonova. "“Hepatogenic diabetes” is an old term and new meaning." Meditsinskiy sovet = Medical Council, no. 7 (May 29, 2020): 19–24. http://dx.doi.org/10.21518/2079-701x-2020-7-19-24.

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Introduction. Today, non-alcoholic fatty liver disease (NAFLD) is increasingly associated with the presence or risk of developing type 2 diabetes (D2). The term “hepatogenic diabetes”, proposed to refer to SD2 in patients with cirrhosis of the liver (CP), has acquired a new meaning, since this combination is of growing interest. The authors analyzed the current literature and summarized data on the pathogenesis, risk factors, and possible therapy of NAFLD.Objective: to evaluate the effectiveness of L-ornithine-L-aspartate (HEPA-Merz, Merz Pharma GmbH & Co) in combination with biguanides in the treatment of NAFLD patients in combination with D2.Materials and methods. The study included 30 patients aged 26 to 60 years with a verified diagnosis of NAFLD of varying degrees of activity in combination with D2. All patients were prescribed combination therapy with the drug HEPA-Merz (“Merz Pharma GmbH and Co”) at a dose of 3 grams 3 times a day in combination with biguanides. The examination was performed on the 1st, 28-th and 56-th days of treatment. In order to determine the effectiveness of therapy, we evaluated the dynamics of clinical symptoms (asthenovegetative, dyspeptic syndromes, pain syndrome in the ball system), biochemical parameters of liver function (changes in markers of cytolysis, cholestasis), lipidogram indicators, glucose levels, glycated hemoglobin, and ultrasound results of abdominal organs.Results. On day 56, asthenovegetative, dyspeptic and pain syndrome were stopped during therapy. Most patients showed a decrease in body weight from 3 to 5 kg. When evaluating changes in biochemical parameters on the 28-th day, the activity of ALT, AST, GGTP and glucose levels significantly decreased against the background of the therapy. On the 56th day of treatment, the activity of transaminases, bilirubin, GGTP and GFR in all patients were within the reference values.Conclusion. Understanding the multifactorial nature of NAFLD and the mechanisms of associated diseases, including D2, will allow us to assess the prognosis of the disease and prescribe adequate timely therapy. The effectiveness of the original ornithineaspartate (Merz Pharma GmbH & Co.) is manifested by a decrease in the processes of cytolysis of hepatocytes, normalization of lipid and carbohydrate metabolism.

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40

Vlokh, I., L. Zhivotovska, S. Mikhnyak, and N. Petryna. "Hepa-Merz in treatment of alcohol and drug-induced encephalopathy." European Psychiatry 17 (May 2002): 210. http://dx.doi.org/10.1016/s0924-9338(02)80897-5.

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41

Yamaguchi, Tsuyoshi, and Tatsuro Matsuoka. "Translation-orientation coupling and Cox-Merz rule of liquid hexane." Journal of Chemical Physics 149, no. 20 (November 28, 2018): 204502. http://dx.doi.org/10.1063/1.5051680.

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42

&NA;. "The efficacy of Merz??s memantine in dementia is confirmed." Inpharma Weekly &NA;, no. 805 (September 1991): 14–15. http://dx.doi.org/10.2165/00128413-199108050-00037.

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43

Schulz, S. "Corrosion Books: Langenscheidt/Routledge Fachwörterbuch Kompakt Englisch. By: Ludwig Merz." Materials and Corrosion 55, no. 4 (April 2004): 319. http://dx.doi.org/10.1002/maco.200490031.

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44

Kleinwechter, H. "Antwort der Leitlinien-Expertengruppe zum Kommentar von Merz und Gembruch." Diabetologie und Stoffwechsel 7, no. 05 (November 14, 2012): 395–96. http://dx.doi.org/10.1055/s-0032-1325430.

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45

FRIEDMAN, TRUDY. "Merz Aesthetics Partnership in Practice conference: The King's Fund, London." Journal of Aesthetic Nursing 3, no. 4 (May 2014): 198. http://dx.doi.org/10.12968/joan.2014.3.4.198.

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46

Tam, K. C., and C. Tiu. "Modified cox-merz rule for charged polymer systems in solution." Journal of Macromolecular Science, Part B 33, no. 2 (May 1994): 173–84. http://dx.doi.org/10.1080/00222349408248086.

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47

Vernon-Carter, E. J., G. Avila-de la Rosa, H. Carrillo-Navas, Y. Carrera, and J. Alvarez-Ramirez. "Cox–Merz rules from phenomenological Kelvin–Voigt and Maxwell models." Journal of Food Engineering 169 (January 2016): 18–26. http://dx.doi.org/10.1016/j.jfoodeng.2015.08.005.

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Lange, Barbara. "Leonie Krutzinna: Der norwegische Schwitters. Die Merz-Kunst im Exil." European Journal of Scandinavian Studies 51, no. 1 (April 1, 2021): 186–90. http://dx.doi.org/10.1515/ejss-2020-2025.

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Schelert, James, Melissa Drozda, Vidula Dixit, Amanda Dillman, and Paul Blum. "Regulation of Mercury Resistance in the Crenarchaeote Sulfolobus solfataricus." Journal of Bacteriology 188, no. 20 (October 1, 2006): 7141–50. http://dx.doi.org/10.1128/jb.00558-06.

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Abstract:

ABSTRACT Mercuric ion, Hg(II), inactivates generalized transcription in the crenarchaeote Sulfolobus solfataricus. Metal challenge simultaneously derepresses transcription of mercuric reductase (merA) by interacting with the archaeal transcription factor aMerR. Northern blot and primer extension analyses identified two additional Hg(II)-inducible S. solfataricus genes, merH and merI (SSO2690), located on either side of merA. Transcription initiating upstream of merH at promoter merHp was metal inducible and extended through merA and merI, producing a merHAI transcript. Northern analysis of a merRA double mutant produced by linear DNA recombination demonstrated merHp promoter activity was dependent on aMerR to overcome Hg(II) transcriptional inhibition. Unexpectedly, in a merA disruption mutant, the merH transcript was transiently induced after an initial period of Hg(II)-mediated transcription inhibition, indicating continued Hg(II) detoxification. Metal challenge experiments using mutants created by markerless exchange verified the identity of the MerR binding site as an inverted repeat (IR) sequence overlapping the transcription factor B binding recognition element of merHp. The interaction of recombinant aMerR with merHp DNA, studied using electrophoretic mobility shift analysis, demonstrated that complex formation was template specific and dependent on the presence of the IR sequence but insensitive to Hg(II) addition and site-specific IR mutations that relieved in vivo merHp repression. Despite containing a motif resembling a distant ArsR homolog, these results indicate aMerR remains continuously DNA bound to protect and coordinate Hg(II)-responsive control over merHAI transcription. The new genetic methods developed in this work will promote experimental studies on S. solfataricus and other Crenarchaeota.

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Gisler, Alois. "The Estimation Error in the Chain-Ladder Reserving Method: A Bayesian Approach." ASTIN Bulletin 36, no. 02 (November 2006): 554–65. http://dx.doi.org/10.2143/ast.36.2.2017939.

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