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Journal articles on the topic 'Mesenchymal stem cells derived Chondrogenic Autologous Treatment'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Leal Reis, Inês, Bruna Lopes, Patrícia Sousa, et al. "Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies—Proof of Concept." Animals 13, no. 8 (2023): 1312. http://dx.doi.org/10.3390/ani13081312.

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Tendon and ligament injuries are frequent in sport horses and humans, and such injuries represent a significant therapeutic challenge. Tissue regeneration and function recovery are the paramount goals of tendon and ligament lesion management. Nowadays, several regenerative treatments are being developed, based on the use of stem cell and stem cell-based therapies. In the present study, the preparation of equine synovial membrane mesenchymal stem cells (eSM-MSCs) is described for clinical use, collection, transport, isolation, differentiation, characterization, and application. These cells are

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2

Smith, John Kelly. "Exercise as an Adjuvant to Cartilage Regeneration Therapy." International Journal of Molecular Sciences 21, no. 24 (2020): 9471. http://dx.doi.org/10.3390/ijms21249471.

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This article provides a brief review of the pathophysiology of osteoarthritis and the ontogeny of chondrocytes and details how physical exercise improves the health of osteoarthritic joints and enhances the potential of autologous chondrocyte implants, matrix-induced autologous chondrocyte implants, and mesenchymal stem cell implants for the successful treatment of damaged articular cartilage and subchondral bone. In response to exercise, articular chondrocytes increase their production of glycosaminoglycans, bone morphogenic proteins, and anti-inflammatory cytokines and decrease their product

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Diomede, Francesca, Thangavelu Soundara Rajan, Marco D’Aurora, et al. "Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study." Stem Cells International 2017 (December 14, 2017): 1–14. http://dx.doi.org/10.1155/2017/1606125.

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Multiple sclerosis (MS) is the most prevalent and progressive autoimmune disease that affects the central nervous system, and currently, no drug is available for the treatment. Stem cell therapy has received substantial attention in MS treatment. Recently, we demonstrated the immunosuppressive effects of mesenchymal stem cells derived from neural crest-originated human periodontal ligament tissue (hPDLSCs) in an in vivo model of MS. In the present study, we comparatively investigated the stemness properties of hPDLSCs derived from healthy donors and relapsing-remitting MS patients. Stem cell m

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Muñoz-Criado, Ignacio, Jose Meseguer-Ripolles, Maravillas Mellado-López, et al. "Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis." Stem Cells International 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/4758930.

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Cartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown to restore degenerated cartilage; however, regenerative differences of ASC would depend on the source of adipose tissue. The infra- and suprapatellar fat pads surrounding the knee offer a potential autologous source of ASC for patients after complete joint substitution. When infrapatellar- and supra

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Mata, Manuel, Lara Milian, Maria Oliver, et al. "In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study." Stem Cells International 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/8309256.

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Osteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage in particular, are affected. The poor regeneration capacity of this tissue together with the lack of pharmacological treatment has led to the development of regenerative medicine methodologies including microfracture and autologous chondrocyte implantation (ACI). The effectiveness of ACI has been shown in vitro and in vivo, but the use of other cell types, including bone marrow and adipose-derived mesenchymal stem cells, is necessary because of the poor proliferation rate of isolated articular ch

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Feng, Y., M. Zhu, S. Dangelmajer, et al. "Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer." Cell Death & Disease 5, no. 12 (2014): e1567-e1567. http://dx.doi.org/10.1038/cddis.2014.521.

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Abstract Adult human adipose-derived mesenchymal stem cells (hAMSCs) are multipotent cells, which are abundant, easily collected, and bypass the ethical concerns that plague embryonic stem cells. Their utility and accessibility have led to the rapid development of clinical investigations to explore their autologous and allogeneic cellular-based regenerative potential, tissue preservation capabilities, anti-inflammatory properties, and anticancer properties, among others. hAMSCs are typically cultured under ambient conditions with 21% oxygen. However, physiologically, hAMSCs exist in an environ

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Lubis, Andri Maruli Tua, Adrian Situmeang, Anissa Feby Canintika, et al. "Intra-articular injection of human umbilical cord-derived mesenchymal stem cells in sheep models of meniscectomy-induced osteoarthritis: An experimental study." Orthopaedic Journal of Sports Medicine 11, no. 2_suppl (2023): 2325967121S0091. http://dx.doi.org/10.1177/2325967121s00913.

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Introduction: Autologous mesenchymal stem cells (MSC) implantation suspended in hyaluronic acid (HA) has been proven to delay worsening of articular cartilage degeneration in the knee joint. This has been the treatment of choice for young patients with knee osteoarthritis (KOA); however, it is associated with morbidities such as requirement of multiple procedures, donor site morbidity and failure in achieving cultured cells with chondrogenic properties. Our study investigates the possibility of direct intra articular injection of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) f

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Francesco, Francesco De, Silvia Mannucci, Giamaica Conti, Elena Dai Prè, Andrea Sbarbati, and Michele Riccio. "A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results." International Journal of Molecular Sciences 19, no. 7 (2018): 2061. http://dx.doi.org/10.3390/ijms19072061.

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Adipose tissue possesses phenotypic gene expression characteristics that are similar to human mesenchymal stem cells (hMSCs). Nevertheless, the multilineage potential may be inhibited, and cells may not expand adequately to satisfy the requirements of Good Manufacturing Practice (cGMP). An autologous hMSC-enriched fat product would fulfil the void from a biomedical and clinical perspective. In this study, we suggest a novel mechanism using a closed system without enzymes, additives or other modifications, which will produce non-expanded, accessible material. This decentralized fat product, unl

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Oliveros Anerillas, Luis, Paul J. Kingham, Mikko J. Lammi, Mikael Wiberg, and Peyman Kelk. "Three-Dimensional Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells Promotes Matrix Metallopeptidase 13 (MMP13) Expression in Type I Collagen Hydrogels." International Journal of Molecular Sciences 22, no. 24 (2021): 13594. http://dx.doi.org/10.3390/ijms222413594.

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Autologous bone transplantation is the principal method for reconstruction of large bone defects. This technique has limitations, such as donor site availability, amount of bone needed and morbidity. An alternative to this technique is tissue engineering with bone marrow-derived mesenchymal stem cells (BMSCs). In this study, our aim was to elucidate the benefits of culturing BMSCs in 3D compared with the traditional 2D culture. In an initial screening, we combined BMSCs with four different biogels: unmodified type I collagen (Col I), type I collagen methacrylate (ColMa), an alginate and cellul

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Miguelino, Maricel G., Christopher D. Pivetti, Lee Lankford, Jerry Sherman Powell, and Aijun Wang. "In Utero Chorionic Villus Stem Cell Transplantation for the Treatment of Hemophilia A." Blood 124, no. 21 (2014): 106. http://dx.doi.org/10.1182/blood.v124.21.106.106.

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Abstract Pregnancy epitomizes graft tolerance, where the intrauterine allogeneic fetus is protected from rejection by the maternal immune system. Fetal cells are able to enter the maternal circulation as early as six weeks of gestation, and persist for decades without graft rejection. We hypothesize that a window of opportunity exists prior to maturation of T cells where in utero transplantation of donor cells may be recognized as self, therefore inducing tolerance permissive for long term engraftment. Hemophilia A (HA) is an X-linked recessive coagulation disorder that results in bleeding dia

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Tolar, Jakub, In-Hyun Park, Lily Xia, et al. "Patient-Specific Induced Pluripotent Stem Cells in Hurler Syndrome." Blood 112, no. 11 (2008): 386. http://dx.doi.org/10.1182/blood.v112.11.386.386.

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Abstract Hurler syndrome (HS; mucopolysaccharidosis type I) is caused by severe mutations in the iduronidase (IDUA) gene, leading to multi-organ system dysfunction due to the toxic accumulation of glycosaminoglycans. Although allogeneic hematopoietic cell transplantation (HCT) has been shown to provide the IDUA protein and to reverse many of the manifestations of HS, allogeneic HCT is associated with significant morbidity and mortality. We hypothesized that an advantageous alternative strategy may be to induce gene-corrected autologous pluripotent cells to become hematopoietic stem cells, whic

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Hébraud, Benjamin, Elodie Labat, Nicolas Espagnolle, Jill Corre, Michel Attal, and Philippe Bourin. "Comparison of Stromal Cells of Adipose Tissue From Multiple Myeloma Patients and Healthy Donors." Blood 116, no. 21 (2010): 2979. http://dx.doi.org/10.1182/blood.v116.21.2979.2979.

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Abstract Abstract 2979 Introduction: Multiple myeloma (MM) is a B-cell neoplasia characterized by the accumulation of malignant plasma cells in the bone marrow (BM) patients causing severe bone disease (with osteolytic lesions, pain, pathological fractures) and cytopenias. These lesions are irreversible, even for patients in complete response to treatment. We have proposed to treat those lesions with mesenchymal stem cells (MSC) of the BM using their ability to differentiate into osteoblasts and to support haematopoiesis. But our previous results demonstrated that MM MSC are abnormal. We thus

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Rao, Shama, Siddharth Shetty, Narendra Nitilapura, Veena Shetty, Guruprasad Kanive Parashiva, and Mohana Kumar Basavarajappa. "Characterization of synovium-derived mesenchymal stem cells from joints with osteochondritis dissecans." Biomedical Research and Therapy 7, no. 10 (2020): 4075–85. http://dx.doi.org/10.15419/bmrat.v7i10.645.

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Introduction: Osteochondritis dissecans (OCD) is a pathologic condition that occurs in children as well as adults. OCD is often managed based on the extent of ischemia and the stage of the disease. Synovial tissue collected during an arthroscopic procedure might serve as an ideal source for autologous mesenchymal stem cells (MSCs) with a potential for regenerative medicine of cartilage. Therefore, the present in vitro study aimed to evaluate the potency characteristics of synovium-derived MSCs (SMSCs) from joints with OCD for prospective autologous therapy. Methods: Primary culture of SM

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Schumann, D., R. Kujat, J. Zellner, et al. "Treatment of human mesenchymal stem cells with pulsed low intensity ultrasound enhances the chondrogenic phenotype in vitro." Biorheology: The Official Journal of the International Society of Biorheology 43, no. 3-4 (2006): 431–43. http://dx.doi.org/10.1177/0006355x2006043003004026.

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This study examined the effects of low intensity pulsed ultrasound (LIPUS) on human bone marrow-derived mesenchymal stem cells undergoing chondrogenic differentiation. Aggregates of mesenchymal stem cells and mesenchymal stem cells seeded in three dimensional matrices were cultured in a defined chondrogenic medium and subjected to LIPUS for the first 7 days of culture. At 1, 7, 14 and 21 days, samples were harvested for histology, immunohistochemistry, RT-PCR, and quantitative DNA and matrix macromolecule analysis. Cell aggregates with daily treatment for 20 minutes showed no significant diffe

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Jiang, Tongmeng, Guojie Xu, Qiuyan Wang, et al. "In vitro expansion impaired the stemness of early passage mesenchymal stem cells for treatment of cartilage defects." Cell Death & Disease 8, no. 6 (2017): e2851-e2851. http://dx.doi.org/10.1038/cddis.2017.215.

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Abstract In vitro cultured autologous mesenchymal stem cells (MSCs) within passage 5 have been approved for clinical application in stem cell-based treatment of cartilage defects. However, their chondrogenic potential has not yet been questioned or verified. In this study, the chondrogenic potential of bone marrow MSCs at passage 3 (P3 BMSCs) was investigated both in cartilage repair and in vitro, with freshly isolated bone marrow mononuclear cells (BMMNCs) as controls. The results showed that P3 BMSCs were inferior to BMMNCs not only in their chondrogenic differentiation ability but also as c

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Huang, Liangjie, Lingxian Yi, Chunli Zhang та ін. "Synergistic Effects of FGF-18 and TGF-β3 on the Chondrogenesis of Human Adipose-Derived Mesenchymal Stem Cells in the Pellet Culture". Stem Cells International 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/7139485.

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Cell-based therapy serves as an effective way for cartilage repair. Compared with a limited source of autologous chondrocytes, adipose-derived stem cells (ADSCs) are proposed as an attractive cell source for cartilage regeneration. How to drive chondrogenic differentiation of ADSCs efficiently remains to be further investigated. TGF-β3 has shown a strong chondrogenic action on ADSCs. Recently, fibroblast growth factor 18 (FGF-18) has gained marked attention due to its anabolic effects on cartilage metabolism, but existing data regarding the role of FGF-18 on the chondrogenic potential of mesen

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Na, Kyung-Hwan, Hyun-Jin Lee, Ju-Hwan Kim, et al. "Enhancement of Chondrogenic Differentiation in Bone Marrow-Derived Stem Cell Spheroids by Cuminum cyminum Methanolic Extract: Insights into Concentration-Dependent mRNA Expression and Gene Clustering Analysis." Journal of Personalized Medicine 14, no. 12 (2024): 1142. https://doi.org/10.3390/jpm14121142.

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Background/Objectives: Cuminum cyminum L. has been utilized as a medicinal plant for centuries. This research sought to examine the effects of cumin methanolic extract (CMT) on the chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells. Methods: Spheroids were generated using human stem cells and cultured with CMT at concentrations between 0 and 1 µg/mL. Morphological assessments and cell viability tests were conducted on days 1 and 3. Chondrogenic differentiation expression was evaluated through quantitative polymerase chain reaction, Western blot, and RNA sequencing

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Wu, Jiang, Guang Hui Wang, Hong Zhang, et al. "Chondrogenic Ability of Bone Marrow Mesenchymal Stem Cells in Alginate and Collagen Sponge." Key Engineering Materials 474-476 (April 2011): 1935–38. http://dx.doi.org/10.4028/www.scientific.net/kem.474-476.1935.

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In the present study, we have demonstrated that alginate and collagen sponge can act as scaffolds in order to support 3-dimensional structure for the differentiated bone marrow derived mesenchymal stem cells (BMSCs) during chondrogenesis in vitro and in vivo. The chondrogenic induced BMSCs were well distributed and differentiation in scaffolds system before implantation, then they produced sufficient ECM in the implants to form chondroid aggregates in vivo. In our opinion, well-differentiated BMSCs is a crucial feature of cartilage repair and only can be achieved in scaffold matrix. Furthermor

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SAFSHEKAN, FARZANEH, MOHAMMAD TAFAZZOLI SHADPOUR, MOHAMMAD ALI SHOKRGOZAR, NOOSHIN HAGHIGHIPOUR, and SEYED HAMED ALAVI. "EFFECTS OF SHORT-TERM CYCLIC HYDROSTATIC PRESSURE ON INITIATING AND ENHANCING THE EXPRESSION OF CHONDROGENIC GENES IN HUMAN ADIPOSE-DERIVED MESENCHYMAL STEM CELLS." Journal of Mechanics in Medicine and Biology 14, no. 04 (2014): 1450054. http://dx.doi.org/10.1142/s0219519414500547.

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Cartilage tissue engineering is a promising treatment for damaged or diseased cartilage that requires thorough understanding of influential parameters involved in chondrogenic differentiation. This study examined how 4-h application of cyclic hydrostatic pressure (CHP) of 5 MPa at 0.5 Hz could modulate chondroinduction of human adipose-derived mesenchymal stem cells (hAMSCs) in vitro. Four groups were examined including a negative control group, a chemical group treated by growth factor for 10 days, a mechanical group exposed to 4-h loading on the 10th day of pellet culture without any chondro

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Ryu, Jae-Sung, Sang Young Seo, Eun-Jeong Jeong, et al. "Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors." International Journal of Molecular Sciences 21, no. 6 (2020): 1967. http://dx.doi.org/10.3390/ijms21061967.

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Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage. Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules. These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition. Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation. However, the role of gangliosides in chondrogenesis is not understood. In this

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Bagge, Jasmin, Haider Mahmood, Jennifer Janes, et al. "Chondrogenic and Osteogenic In Vitro Differentiation Performance of Unsorted and Sorted CD34+, CD146+, and CD271+ Stem Cells Derived from Microfragmented Adipose Tissue of Patients with Knee Osteoarthritis." Journal of Clinical Medicine 14, no. 4 (2025): 1184. https://doi.org/10.3390/jcm14041184.

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Background/Objectives: Treatment of knee osteoarthritis (OA) with autologous stem cells from microfragmented adipose tissue (MFAT) has shown promising but varying results. Multiple stem cell types, including CD34+, CD146+, and CD271+ stem cells, have been identified within MFAT. Patient-specific heterogeneity in stem cell populations and the content of highly potent cells may be determining factors for a successful treatment outcome. The current study aimed to identify the most promising stem cell type in MFAT to treat OA, focusing on their chondrogenic and osteogenic differentiation performan

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Bräunig, P., W. G. Glanzner, V. B. Rissi, and P. B. D. Gonçalves. "The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70, no. 1 (2018): 160–68. http://dx.doi.org/10.1590/1678-4162-9132.

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ABSTRACT The adipose tissue is a reliable source of Mesenchymal stem cells (MSCs) showing a higher plasticity and transdifferentiation potential into multilineage cells. In the present study, adipose tissue-derived mesenchymal stem cells (AT-MSCs) were isolated from mice omentum and epididymis fat depots. The AT-MSCs were initially compared based on stem cell surface markers and on the mesodermal trilineage differentiation potential. Additionally, AT-MSCs, from both sources, were cultured with differentiation media containing retinoic acid (RA) and/or testicular cell-conditioned medium (TCC).

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Facchini, Andrea, Gina Lisignoli, Sandra Cristino, et al. "Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold." Biorheology: The Official Journal of the International Society of Biorheology 43, no. 3-4 (2006): 471–80. http://dx.doi.org/10.1177/0006355x2006043003004029.

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The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treat

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Garcia, John, Claire Mennan, Helen S. McCarthy, Sally Roberts, James B. Richardson, and Karina T. Wright. "Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat." Stem Cells International 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/6969726.

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Autologous chondrocyte implantation (ACI) is a cell-based therapy that has been used clinically for over 20 years to treat cartilage injuries more efficiently in order to negate or delay the need for joint replacement surgery. In this time, very little has changed in the ACI procedure, but now many centres are considering or using alternative cell sources for cartilage repair, in particular mesenchymal stem cells (MSCs). In this study, we have tested the chondrogenic potential of donor-matched MSCs derived from bone marrow (BM), infrapatellar fat pad (FP), and subcutaneous fat (SCF), compared

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Altaie, A., E. Jones, O. Wall, and D. Mcgonagle. "FRI0389 TOWARDS ONE-STAGE ARTICULAR CARTILAGE REPAIR USING DEVICE BASED ARTHROSCOPIC MECHANICAL RELEASE OF SYNOVIAL MESENCHYMAL STEM CELLS WITH CHONDROGENESIS AUGMENTATION WITH AUTOLOGOUS PLATELET CONCENTRATE WITHOUT CELL CULTURE EXPANSION." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 792–93. http://dx.doi.org/10.1136/annrheumdis-2020-eular.5717.

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Background:Synovial fluid contains resident mesenchymal stem cells (SF-MSCs) that are derived from the synovial membrane and may interact with superficial cartilage injury sites. We previously reported on a novel methodology for increasing the number of MSCs in the knee joints using synovial brushing combined with platelet lysate (PL) as a chondrogenic inducer [1, 2].Objectives:The purpose of this study was to evaluate autologous and allogenic PL as a chondrogenic inducer and the chondrogenic potential of the mobilised MSCs without further ex vivo expansion. The desired goal of the study was t

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Vakhrushev, Igor V., Yulia B. Basok, Konstantin K. Baskaev, et al. "Cartilage-Specific Gene Expression and Extracellular Matrix Deposition in the Course of Mesenchymal Stromal Cell Chondrogenic Differentiation in 3D Spheroid Culture." International Journal of Molecular Sciences 25, no. 11 (2024): 5695. http://dx.doi.org/10.3390/ijms25115695.

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Articular cartilage damage still remains a major problem in orthopedical surgery. The development of tissue engineering techniques such as autologous chondrocyte implantation is a promising way to improve clinical outcomes. On the other hand, the clinical application of autologous chondrocytes has considerable limitations. Mesenchymal stromal cells (MSCs) from various tissues have been shown to possess chondrogenic differentiation potential, although to different degrees. In the present study, we assessed the alterations in chondrogenesis-related gene transcription rates and extracellular matr

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Malik, H., V. Sharma, S. Saini, S. Guha, and D. Malakar. "225 AUTOLOGOUS TRANSPLANTATION OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE IN ANIMAL." Reproduction, Fertility and Development 28, no. 2 (2016): 244. http://dx.doi.org/10.1071/rdv28n2ab225.

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The present study was carried out for isolation and culture of adipose tissue-derived mesenchymal stem cells of goat (gADSC) and dogs (1 dog was suffering from hip dysplasia and another dog from paraplegia) and their characterisation with different markers. Adipose tissue of goat and dog were aseptically isolated and treated with collagenase for 2 h in a CO2 incubator. The enzymatic digested cells were filtered through a 41-µm filter and cells were resuspended in cell culture flask containing medium DMEM/F12, 10% fetal bovine serum, and 50 μg mL–1 gentamycin. In vitro-cultured ADSC were charac

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Lange-Consiglio, Anna, Filippo Tagliasacchi, Fausto Cremonesi, et al. "Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD)." Animals 15, no. 2 (2025): 242. https://doi.org/10.3390/ani15020242.

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Urine-derived mesenchymal stromal/stem cells (USCs) could be a valuable source of cells in regenerative medicine because urine can be easily collected non-invasively. In this paper, USCs were isolated from both healthy dogs and dogs affected by chronic kidney disease (CKD), and the efficacy of collection methods (spontaneous micturition, bladder catheterization, and cystocentesis) were compared. Isolated cells were cultured in the presence of platelet-rich plasma and studied for their proliferative capacity (growth curve, doubling time, and colony forming unit), differentiation properties, exp

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Rim, Yeri Alice, Yoojun Nam, Narae Park, et al. "Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors." Cells 9, no. 3 (2020): 582. http://dx.doi.org/10.3390/cells9030582.

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Human degenerative cartilage has low regenerative potential. Chondrocyte transplantation offers a promising strategy for cartilage treatment and regeneration. Currently, chondrogenesis using human pluripotent stem cells (hiPSCs) is accomplished using human recombinant growth factors. Here, we differentiate hiPSCs into chondrogenic pellets using minicircle vectors. Minicircles are a non-viral gene delivery system that can produce growth factors without integration into the host genome. We generated minicircle vectors containing bone morphogenetic protein 2 (BMP2) and transforming growth factor

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Marędziak, Monika, Krzysztof Marycz, Krzysztof A. Tomaszewski, Katarzyna Kornicka, and Brandon Michael Henry. "The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells." Stem Cells International 2016 (2016): 1–15. http://dx.doi.org/10.1155/2016/2152435.

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Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic differentiation potential of mesenchymal stem cells. The aim of this study was to assess the age-related maintenance of physiological function and differentiation potential of hASCs in vitro. hASCs were isolated from patients of four different age groups: (1) >20 years (n=7), (2) >50 years (n=7),

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Jakob, Yvonne, Johann Kern, David Gvaramia, et al. "Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction." Cells 13, no. 2 (2024): 141. http://dx.doi.org/10.3390/cells13020141.

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Tissue engineering (TE) techniques offer solutions for tissue regeneration but require large quantities of cells. For microtia patients, TE methods represent a unique opportunity for therapies with low donor-site morbidity and reliance on the surgeon’s individual expertise. Microtia-derived chondrocytes and perichondrocytes are considered a valuable cell source for autologous reconstruction of the pinna. The aim of this study was to investigate the suitability of perichondrocytes from microtia patients for autologous reconstruction in comparison to healthy perichondrocytes and microtia chondro

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Pershina, Polina A., Yuri A. Novosad, Kristina N. Rodionova, et al. "Use of autologous mesenchymal stem cells derived from adipose tissue for the treatment of hyaline cartilage defects in laboratory animals: a literature review." Pediatric Traumatology, Orthopaedics and Reconstructive Surgery 12, no. 4 (2024): 499–510. https://doi.org/10.17816/ptors635941.

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BACKGROUND: Degenerative/dystrophic cartilage diseases are an epidemiologically important challenge in modern traumatology and orthopedics. The use of autologous mesenchymal stem cells to repair cartilage defects is currently the most promising treatment option. AIM: The aim of this paper was to review the literature on the use of adipose tissue-derived mesenchymal stem cells to repair articular cartilage defects in laboratory animals. MATERIALS AND METHODS: Recent and historical Russian and English literature was searched in Google Scholar, Cyberleninka, PubMed, eLibrary, Mendeley, Science Di

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Nicpoń, J., K. Marycz, and J. Grzesiak. "Therapeutic effect of adipose-derived mesenchymal stem cell injection in horses suffering from bone spavin." Polish Journal of Veterinary Sciences 16, no. 4 (2013): 753–54. http://dx.doi.org/10.2478/pjvs-2013-0107.

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Abstract In this article we demonstrate the efficiency of autologous transplantations of adipose-derived mesenchymal stem cells for equine bone spavin treatment. Horses qualified to the study were divided into three groups: (i) research - treated with intra-articular injections of autologous stem cells, (ii) comparison treated with steroid drugs and (iii) control - untreated. All animals underwent comprehensive clinical examination before and after treatment. Our research confirms the long-term beneficial influence resulting from stem cell therapy in horse bone spavin treatment, in contrast to

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Mallam, Elizabeth, Kevin Kemp, Alastair Wilkins, Claire Rice, and Neil Scolding. "Characterization of in vitro expanded bone marrow-derived mesenchymal stem cells from patients with multiple sclerosis." Multiple Sclerosis Journal 16, no. 8 (2010): 909–18. http://dx.doi.org/10.1177/1352458510371959.

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Recent studies have investigated the potential of autologous bone marrow-derived mesenchymal stem cells (MSCs) as a therapy for multiple sclerosis. Whether MSCs from individuals with multiple sclerosis are functionally and/or phenotypically abnormal has received less attention. Through our Phase I clinical trial, SIAMMS, we were able to isolate and characterize MSCs from individuals with multiple sclerosis. The objective of the study was to demonstrate that MSCs from individuals with multiple sclerosis show no significant differences from MSCs derived from individuals without multiple sclerosi

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Miletic, Maja, S. Mojsilovic, Ivana Okic-Djordjevic, et al. "Mesenchymal stem cells isolated from human periodontal ligament." Archives of Biological Sciences 66, no. 1 (2014): 261–71. http://dx.doi.org/10.2298/abs1401261m.

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Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, v

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Dinh, Han Van. "ID3015 Mesenchymal stem cells for treatment of chronic wounds: A Study with autologous transplantation of adipose-derived stem cells." Biomedical Research and Therapy 4, S (2017): 27. http://dx.doi.org/10.15419/bmrat.v4is.236.

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Objective: This study was to determine the effects of adipose-derived stem cells (ADSCs) on dermal fibroblasts responses to injury including migration and proliferation in vitro. We also evaluated the autologous transplantation of ADSCs on treatment of human chronic wounds. Subjects and methods: The proliferation and migration of fibroblast was evaluated by co-culture ADSCs with allogenic dermal fibroblast and by the scratch assay. In clinical study, autologous ADSCs were transplanted on to chronic wounds of 25 patients, who were hospitalized into the Wound Healing Department of the Nati

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Jakob, Mark, Mario Hambrecht, Jennifer L. Spiegel, et al. "Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Show Comparable Functionality to Their Autologous Origin." Cells 10, no. 1 (2020): 33. http://dx.doi.org/10.3390/cells10010033.

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A multimodal therapeutic approach involving radiotherapy is required when treating head and neck squamous cell carcinoma. However, radiotherapy is restricted due to its high risk for damages to the surrounding healthy tissue of the treated area. Tissue regeneration and wound healing is promoted by the survival and regenerative capacities of tissue-resident or invading stem cells. Mesenchymal stem cells (MSCs) exhibit a promising therapeutic potential in the field of cell-based tissue engineering and regenerative medicine due to their immunomodulatory properties and differentiation capacity. Ho

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Dao, Thuy Thi Thanh, Liem Hieu Phan, Ha Thi Ngan Le, Lan Thi Phi, Phuc Van Pham, and Ngoc Bich Vu. "ID: 1076 Establishing an artificial cartilage from rabbit bone marrow derived mesenchymal stem cell and polycaprolactone scaffold." Biomedical Research and Therapy 4, S (2017): 161. http://dx.doi.org/10.15419/bmrat.v4is.350.

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Nowaday, the regenerative medicine based on the use of biomaterial scaffold combine to stem cells was considered as a necessary treatment strategy for injury or diseases involving cartilage. In this study, we investigated the adherence, proliferation and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (rBM-MSCs) on biodegradable polycaprolactone scaffold (PCL). MSCs were isolated from bone marrow of rabbit. rBM-MSC (2x105cell/scaffold) were seeded on PCL and collagen-coated PCL (PCL/col) scaffolds. The cell adhesion and proliferation abilities on the PCL and P

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Giglio, Pedro Nogueira, Débora Levy, Phelipe Oliveira Favaron, et al. "Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients." Cells 14, no. 11 (2025): 830. https://doi.org/10.3390/cells14110830.

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Cartilage-derived migratory cells show great potential for autologous use in cartilage repair surgery. However, their collection through arthroscopic biopsy has not been previously reported in individuals without osteoarthritis. This study aimed to characterize migratory cartilage cells isolated from arthroscopic biopsies of volunteers without osteoarthritis and compare them with cells obtained by enzymatic digestion. Cell cultures were successfully established using both methods—enzymatic digestion and cell migration—from cartilage explants, with no significant differences observed in stem ce

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Mediano, Diego R., David Sanz-Rubio, Rosa Bolea, et al. "Characterization of mesenchymal stem cells in sheep naturally infected with scrapie." Journal of General Virology 96, no. 12 (2015): 3715–26. http://dx.doi.org/10.1099/jgv.0.000292.

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Mesenchymal stem cells (MSCs) can be infected with prions and have been proposed as in vitro cell-based models for prion replication. In addition, autologous MSCs are of interest for cell therapy in neurodegenerative diseases. To the best of our knowledge, the effect of prion diseases on the characteristics of these cells has never been investigated. Here, we analysed the properties of MSCs obtained from bone marrow (BM-MSCs) and peripheral blood (PB-MSCs) of sheep naturally infected with scrapie — a large mammal model for the study of prion diseases. After three passages of expansion, MSCs de

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Barlian, Anggraini, Rizka Musdalifah Amsar, Salindri Prawitasari, Christofora Hanny Wijaya, Ika Dewi Ana, and Triati Dewi Kencana Wungu. "The Properties of Exosomes Derived from Mesenchymal Stem Cells Preconditioned with L-Ascorbic Acid and Cobalt (II) Chloride." HAYATI Journal of Biosciences 30, no. 6 (2023): 1100–1110. http://dx.doi.org/10.4308/hjb.30.6.1100-1110.

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Extracellular vesicles including exosomes, are produced by cells for intracellular communication. Preconditioning of parental cells influences exosome properties. The purpose of this study was to examine the effects of L-ascorbic acid (LAA) and cobalt (II) chloride (CoCl2) on human Wharton’s jelly mesenchymal stem cell (hWJ-MSC)-derived exosomes and their ability to promote stem cell differentiation into chondrocytes. The cells were isolated from the umbilical cord and characterized according to the criteria for mesenchymal stem cell. The cells were cultured in a serum-free medium containing L

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Hudita, Ariana, Bianca Galateanu, Sorina Dinescu, et al. "In Vitro Effects of Cetylated Fatty Acids Mixture from Celadrin on Chondrogenesis and Inflammation with Impact on Osteoarthritis." CARTILAGE 11, no. 1 (2018): 88–97. http://dx.doi.org/10.1177/1947603518775798.

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Objective Cetylated fatty acids are a group of naturally occurring fats of plant and/or animal origin. Cetyl myristoleate, in particular, was initially involved in osteoarthritis related research as its therapeutic administration prevented experimentally induced arthritis in Swiss Albino mice. In this context, the aim of our study was to investigate the possible mechanisms of Celadrin cetylated fatty acids action at the cellular level inflammation related pain relief and chondrogenesis. Design For this, we tested the effects of the cetylated fatty acids mixture from Celadrin on an in vitro sca

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Liao, Hsiu-Jung, Chih-Hung Chang, Chi-Ying F. Huang, and Hui-Ting Chen. "Potential of Using Infrapatellar–Fat–Pad–Derived Mesenchymal Stem Cells for Therapy in Degenerative Arthritis: Chondrogenesis, Exosomes, and Transcription Regulation." Biomolecules 12, no. 3 (2022): 386. http://dx.doi.org/10.3390/biom12030386.

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Infrapatellar fat pad–derived mesenchymal stem cells (IPFP-MSCs) are a type of adipose-derived stem cell (ADSC). They potentially contribute to cartilage regeneration and modulation of the immune microenvironment in patients with osteoarthritis (OA). The ability of IPFP-MSCs to increase chondrogenic capacity has been reported to be greater, less age dependent, and less affected by inflammatory changes than that of other MSCs. Transcription-regulatory factors strictly regulate the cartilage differentiation of MSCs. However, few studies have explored the effect of transcriptional factors on IPFP

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Pawar, Madhura, Vivek Pawar, Apathsakayan Renugalakshmi, et al. "Glucose and Serum Deprivation Led to Altered Proliferation, Differentiation Potential and AMPK Activation in Stem Cells from Human Deciduous Tooth." Journal of Personalized Medicine 12, no. 1 (2021): 18. http://dx.doi.org/10.3390/jpm12010018.

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Stem cell therapy is an evolving treatment strategy in regenerative medicine. Recent studies report stem cells from human exfoliated deciduous teeth could complement the traditional mesenchymal stem cell sources. Stem cells from human exfoliated deciduous teeth exhibit mesenchymal characteristics with multilineage differentiation potential. Mesenchymal stem cells are widely investigated for cell therapy and disease modeling. Although many research are being conducted to address the challenges of mesenchymal stem cell therapy in clinics, most of the studies are still in infancy. Host cell micro

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Purwati, Sony Wibisono, Ari Sutjahjo, T. J. Askandar, and Fedik Abdul Rantam. "Adipose-Derived Mesenchymal Stem Cells for Treatment Tertiary Failure Diabetes Mellitus Type 2." Journal of Biomimetics, Biomaterials and Biomedical Engineering 31 (March 2017): 91–95. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.31.91.

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Recent progress in diabetes mellitus therapy has focused attention on generation of surrogate β-cells from mesenchymal stem cells (MSCs) derived adult tissues. In this study, we conducted a phase I clinical trial in patients with type 2 diabetes mellitus in various age between 30-79 years old who were treated with autologous adipose-derived MSCs transplantation. MSCs injected into diabetic patients through catheterization then we measured levels of fasting blood glucose, 2h postprandial blood glucose, insulin, c-peptide pre and post stem cell transplantation, also HbA1c. Levels of blood glucos

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Gabriel, Kareeba Leefoon, Mohamed Sami, Olumide Ijishakin, et al. "Efficacy and Safety of Stem Cell Therapy in the Treatment of Fistulizing Crohn's Disease and the Role of Autologous Stem Cell Transplantation: A Systematic Review." Journal of Advances in Medicine and Medical Research 35, no. 23 (2023): 189–201. http://dx.doi.org/10.9734/jammr/2023/v35i235294.

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Background: Crohn's Disease (CD), especially its fistulizing form, presents significant treatment challenges. Stem cell transplantation, particularly autologous stem cell transplantation, has emerged as a novel approach for these difficult-to-treat cases. This systematic review concentrates on evaluating the efficacy and safety of stem cell transplantation in treating fistulizing CD. Methods: We searched PubMed, Embase, and Cochrane Library up to June 2023. Both randomized controlled trials and non-randomized clinical trials involving adult patients (18 years or older) with CD and utiliz

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Miyagi-Shiohira, Chika, Yoshiki Nakashima, Naoya Kobayashi, et al. "Induction of Expandable Adipose-Derived Mesenchymal Stem Cells from Aged Mesenchymal Stem Cells by a Synthetic Self-Replicating RNA." International Journal of Molecular Sciences 19, no. 11 (2018): 3489. http://dx.doi.org/10.3390/ijms19113489.

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Adipose-derived mesenchymal stem cells (ADSCs) have attracted attention due to their potential for use in the treatment of various diseases. However, the self-renewal capacity of ADSCs is restricted and their function diminishes during passage. We previously generated induced tissue-specific stem cells from mouse pancreatic cells using a single synthetic self-replicating Venezuelan Equine Encephalitis (VEE)-reprogramming factor (RF) RNA replicon (SR-RNA) expressing the reprogramming factors POU class 5 homeobox 1 (OCT4), Krueppel-like factor 4 (KLF4), Sex determining region Y-box 2 (SOX2), and

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Soleimani, Saleh, Habibollah Nazem, Mohammad Fazilati, Mohammad Pezeshki Modarres, and Seyed Mohammad Atyabi. "Survey differentiation of mesenchymal stem cell to chondrocyte using synovial fluid and coldatmospheric plasma treated nanofibrous pcl substrate." MOJ Biology and Medicine 6, no. 2 (2021): 52–56. http://dx.doi.org/10.15406/mojbm.2021.06.00129.

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Tissue engineering has developed strategy for the repair and regeneration of a variety of tissues. The demand for effective treatment strategies of cartilage cause to autologous human Mesenchymal stem cells (hMSC) used for the repair of cartilage defects. In this study effect of some factors on human MSCs for differentiation to chondrocyte were surveyed such as Present of Poly Ɛ-caprolactone (PCL) nanofibrous scaffold and cold atmospheric plasma (CAP), also effect and amount of synovial fluid were evaluated. In this experimental study cell culture for differentiation assay performed in 6 well

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Roato, Ilaria, Daniela Alotto, Dimas Carolina Belisario, et al. "Adipose Derived-Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue." Stem Cells International 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/4968724.

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Osteoarthritis is characterized by loss of articular cartilage also due to reduced chondrogenic activity of mesenchymal stem cells (MSCs) from patients. Adipose tissue is an attractive source of MSCs (ATD-MSCs), representing an effective tool for reparative medicine, particularly for treatment of osteoarthritis, due to their chondrogenic and osteogenic differentiation capability. The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion, but multiple infusions could be also more efficacious. Here we studied some crucial aspects of adipose tissue banking

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Kreulen, Christopher, Eric Giza, Alvin Shieh, et al. "Effects of Micronized Cartilage Matrix on Cartilage Repair in Osteochondral Lesions of the Talus." Foot & Ankle Orthopaedics 2, no. 3 (2017): 2473011417S0000. http://dx.doi.org/10.1177/2473011417s000055.

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Category: Ankle, Arthroscopy, Sports Introduction/Purpose: A promising new technique in the treatment of osteochondral lesions of the talus (OLT) involves the use of an acellular micronized cartilage matrix (MCM), BioCartilage, to fill the lesions. The micronized cartilage matrix is thought to improve the production of hyaline-like cartilage by resident cells in a cartilage defect, but its effect on bone marrow cells remains untested. Here we hypothesized that adding bone-marrow derived stem cells to the BioCartilage would result in the chondrogenic differentiation of the stem cells. We design

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