Relevant bibliographies by topics / Mesophiles

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  2. Dissertations / Theses
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  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Mesophiles'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Mesophiles"

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Pollo, Stephen M. J., Olga Zhaxybayeva, and Camilla L. Nesbø. "Insights into thermoadaptation and the evolution of mesophily from the bacterial phylum Thermotogae." Canadian Journal of Microbiology 61, no. 9 (September 2015): 655–70. http://dx.doi.org/10.1139/cjm-2015-0073.

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Thermophiles are extremophiles that grow optimally at temperatures >45 °C. To survive and maintain function of their biological molecules, they have a suite of characteristics not found in organisms that grow at moderate temperature (mesophiles). At the cellular level, thermophiles have mechanisms for maintaining their membranes, nucleic acids, and other cellular structures. At the protein level, each of their proteins remains stable and retains activity at temperatures that would denature their mesophilic homologs. Conversely, cellular structures and proteins from thermophiles may not function optimally at moderate temperatures. These differences between thermophiles and mesophiles presumably present a barrier for evolutionary transitioning between the 2 lifestyles. Therefore, studying closely related thermophiles and mesophiles can help us determine how such lifestyle transitions may happen. The bacterial phylum Thermotogae contains hyperthermophiles, thermophiles, mesophiles, and organisms with temperature ranges wide enough to span both thermophilic and mesophilic temperatures. Genomic, proteomic, and physiological differences noted between other bacterial thermophiles and mesophiles are evident within the Thermotogae. We argue that the Thermotogae is an ideal group of organisms for understanding of the response to fluctuating temperature and of long-term evolutionary adaptation to a different growth temperature range.
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Korehi, Hananeh, and Axel Schippers. "Bioleaching of a Marine Hydrothermal Sulfide Ore with Mesophiles, Moderate Thermophiles and Thermophiles." Advanced Materials Research 825 (October 2013): 229–32. http://dx.doi.org/10.4028/www.scientific.net/amr.825.229.

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Marine hydrothermal polymetallic sulfide ores contain high amounts of valuable metals such as Cu, Pb, Zn, Au, Ag, as well as In, Ge, Bi, and Se. Samples from a site in the Indian Ocean were taken during a BGR ship cruise, crushed and sieved for bioleaching experiments to reveal the extraction of the various metals. Chalcopyrite was the main mineral, the total copper content was 38.5 %wt. Comparative bioleaching with mesophilic, moderate thermophilic and thermophilic acidophilic iron- and sulfur-oxidizing bacteria and archaea was investigated. Batch culture experiments were conducted at 2% (w/v) pulp density in shake flasks in the presence of Acidithiobacillus ferrooxidans, Acidiphilium sp. and Acidithiobacillus thiooxidans as mesophiles (30°C), a mixed culture of moderate thermopilic iron- and sulfur oxidizing bacteria (50°C) and the thermophile Acidianus brierleyi (70°C). The results after four weeks showed most effective dissolution of copper in the presence of A. brierleyi (up to 4.3 g/l), compared with moderate thermophiles and mesophiles (3.3 g/l and 2.5 g/l, respectively). Furthermore, the bioleaching performance was approved with dissolved iron concentrations. Conclusively, an increase in temperature from 30 °C to 70 °C had a major impact on bioleaching efficiency. Copper and iron extraction efficiency occurred in the order thermophiles, moderate thermophiles, mesophiles.
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Ramos, Cleusa Fernandes, Andreia Assuncao Soares, Isabela Carvalho dos Santos, Felipe Andre Pereira Ramos, Odair Alberton, Luciana Kazue Otutumi, Lidiane Nunes Barbosa, Ricardo de Melo Germano, Daniela Dib Goncalves, and Luiz Sergio Merlini. "Heat Treatment Reduces Bacterial Contamination of Medicinal Plants Grown in A Community Garden." Journal of Pure and Applied Microbiology 14, no. 3 (September 16, 2020): 1817–23. http://dx.doi.org/10.22207/jpam.14.3.20.

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The verification of microorganisms that indicate contamination of medicinal plants cultivated in vegetable gardens is fundamental to contribute and improve the conditions and management of the garden, providing safer and healthier food for the consumer population. The study aims to evaluate the bacteriological quality of Cymbopogon citratus, Aloysia citriodora, Plectranthus barbalus, Aloe vera (L.) Burm. F., Sedum dendroideum, and Peumus boldus cultivated in community gardens. Six samples were randomly collected from each plant and sent to the Laboratory of Veterinary Preventive Medicine and Public Health at Universidade Paranaense-UNIPAR, for bacteriological analysis (aerobic mesophilic microorganisms, total coliforms and coliforms at 45ºC. The cold extract (room temperature) of the medicinal plants evaluated, the Plectranthus barbalus had the highest mean for aerobic mesophiles (1.26 CFU/g x 106) and the Cymbopogon citratus second highest mean for aerobic mesophiles (9.80 CFU/g x 105). In the coliform count at 45°C, the highest mean was found in the Aloysia citriodora (2.90 CFU/g x 103) and it presented the second lowest mean for coliforms at 45°C (0.63 CFU/g x 103) and was zero for total coliforms. Regarding the hot extract (95°C-100°C) of the medicinal plants evaluated, a significantly higher aerobic mesophiles count was found for Aloysia citriodora when compared with Aloe vera (L.) Burm. F., Sedum dendroideum and Peumus boldus, not differing statistically from Cymbopogon citratus and Plectranthus barbalus. It was found that medicinal plants are contaminated but, since they are indicated as teas (infusion), they are safe for their consumers because the risk of bacteriological contamination can be considerably eliminated or reduced due to the boiling process.
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FUJIKAWA, HIROSHI, and TAKESHI ITOH. "Thermal Inactivation Analysis of Mesophiles Using the Arrhenius and z-Value Models." Journal of Food Protection 61, no. 7 (July 1, 1998): 910–12. http://dx.doi.org/10.4315/0362-028x-61.7.910.

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The Arrhenius and z-value models were compared for thermal inactivation analysis of a mesophilic bacterium. The models produced a linear curve for the thermal inactivation data. Concerning the rate constant of inactivation, the D and k values predicted by the models at a constant temperature were similar. For extrapolated temperatures the z-value model predicted an insignificant decrease in the survival ratio compared to the Arrhenius model. The dynamic temperature survival curves predicted by the models were similar, and the models characterized the results. These results demonstrated that the models can be used for thermal inactivation analysis of mesophiles at various temperatures.
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Seel, Waldemar, Julia Derichs, and André Lipski. "Increased Biomass Production by Mesophilic Food-Associated Bacteria through Lowering the Growth Temperature from 30°C to 10°C." Applied and Environmental Microbiology 82, no. 13 (April 15, 2016): 3754–64. http://dx.doi.org/10.1128/aem.00211-16.

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ABSTRACTFive isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the speciesEscherichia coli,Listeria monocytogenes,Staphylococcus xylosus,Bacillus cereus,Pedobacter nutrimenti, andPedobacter panaciterraewere tested for the effect of growth temperature (30°C and 10°C) on biomass formation. Growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and defined media: viable cell count, total cell count, cell dry weight, whole-cell protein content, and cell morphology. According to the lack of growth at 1°C, all strains were assigned to the thermal class of mesophiles. Glucose and ammonium consumption related to cell yield were analyzed in defined media. Except for the protein content, temperature had a significant (ttest,P< 0.05) effect on all biomass formation parameters for each strain. The results show a significant difference between the isolates and the related reference strains. Isolates achieved an increase in biomass production between 20% and 110% at the 10°C temperature, which is 15 to 25°C lower than their maximum growth rate temperatures. In contrast, reference strains showed a maximum increase of only about 25%, and some reference strains showed no increase or a decrease of approximately 25%. As expected, growth rates for all strains were higher at 30°C than at 10°C, while biomass production for isolates was higher at 10°C than at 30°C. In contrast, the reference strains showed similar growth yields at the two temperatures. This also demonstrates for mesophilic bacterial strains more efficient nutrient assimilation during growth at low temperatures. Until now, this characteristic was attributed only to psychrophilic microorganisms.IMPORTANCEFor several psychrophilic species, increased biomass formation was described at temperatures lower than optimum growth temperatures, which are defined by the highest growth rate. This work shows increased biomass formation at low growth temperatures for mesophilic isolates. A comparison with closely related reference strains from culture collections showed a significantly smaller increase or no increase in biomass formation. This indicates a loss of specific adaptive mechanisms (e.g., cold adaptation) for mesophiles during long-term cultivation. The increased biomass production for mesophiles under low-temperature conditions opens new avenues for a more efficient biotechnological transformation of nutrients to microbial biomass. These findings may also be important for risk assessment of cooled foods since risk potential is often correlated with the cell numbers present in food samples.
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Herrera-Mejía, María Juliana, Alfonso Tullio Sarmiento, and Luz Indira Sotelo-Díaz. "Predictive model of microorganism mesophiles in processed meat products during storage under fluctuating temperatures1." DYNA 86, no. 208 (January 1, 2019): 46–52. http://dx.doi.org/10.15446/dyna.v86n208.66777.

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The aim of this investigation was to develop a predictive model of microorganism mesophiles in processed meat products during storage under fluctuating temperatures between 1°C to 7°C to establish the shelf removal date of the products based on mesophile limits established by Colombian Technical Standard NTC 1325 of 2008 for non-canned processed meat products. The variables used in the model were as follows: temperature, storage time and population of microorganisms at the beginning of storage. The S curve of the growth of the microorganisms was approximated by sections using a multivariate quadratic regression equation. The model achieved 91% accuracy for the prediction of the shelf removal date. In terms of practicality, the model offers a simpler alternative to traditional models for the prediction of microorganisms that require a greater amount of parameters and data.
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Vilcáez, Javier, Koichi Suto, and Chihiro Inoue. "Modeling the Auto-Thermal Performance of a Thermophilic Chalcopyrite Bioleaching Heap Employing Mesophilic and Thermophilic Microbes." Advanced Materials Research 20-21 (July 2007): 70–74. http://dx.doi.org/10.4028/www.scientific.net/amr.20-21.70.

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A model was prepared to study the performance of a thermophilic bioleaching heap that employs mixed mesophilic and thermophilic microbes for copper extraction from CuFeS2. Mesophiles’ preference for and ease of dissolving additional FeS2 provided to the heap enables the transition from a mesophilic to a thermophilic bioleaching state without the necessity of additional energy supply. In this sense, the mathematical description of the bioleaching process is done taking into consideration the dependency of both microbes’ biological states on physicochemical factors such as the temperature and O2 availability. With regard to the flow rates of the liquid and air phases, simulation results have shown that these flow rates govern not just the heat transfer and variation of cell distribution, but also the leaching rate regardless of the fraction of CuFeS2 per FeS2 leached (FCP) which is the other variable influencing to the heat accumulation in the heap.
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PRIETO, MIGUEL, MARÍA L. GARCÍA, MARÍA R. GARCÍA, ANDRÉS OTERO, and BENITO MORENO. "Distribution and Evolution of Bacteria on Lamb Carcasses During Aerobic Storage." Journal of Food Protection 54, no. 12 (December 1, 1991): 945–49. http://dx.doi.org/10.4315/0362-028x-54.12.945.

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The change of bacterial counts during storage life of chilled (3±1°C) lamb carcasses was determined by sampling at 0, 5, 10, and 15 d postslaughter and at the spoilage time. Three sites (neck, brisket, and leg) of each carcass were sampled using three sampling methods (swabbing, scraping, and excision of the swabbed and scraped areas). After slaughter, mean count of mesophiles (30°C) was 4.96 log10 CFU/cm2. The initial mean count of psychrotrophs (7°C) expressed as a percentage of the mean count of mesophiles was 33.4%. The mean values of mesophiles and psychrotrophs associated with spoilage were 7.4 log10/cm2 and 7.95 log10/cm2, respectively. Carcasses always had pH values above 5.8 and kept unspoiled 23–29 d. Brisket and leg were the most contaminated areas. Percentages of bacteria recovered by scraping were higher than those obtained by swabbing (ca. 20%). Bacterial counts were significantly affected by day of storage (p&lt;0.001), sampling point (p&lt;0.001), and temperature of incubation (p&lt;0.05). Interaction between sampling day and sampling site was also observed.
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THOMAS, M. Theresa, and K. Robert SCOPES. "The effects of temperature on the kinetics and stability of mesophilic and thermophilic 3-phosphoglycerate kinases." Biochemical Journal 330, no. 3 (March 15, 1998): 1087–95. http://dx.doi.org/10.1042/bj3301087.

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The effects of temperature on the kinetic parameters kcat and Km, for three isolates of the highly conserved monomeric enzyme 3-phosphoglycerate kinase (PGK), were investigated in detail using a rapid automated kinetics apparatus. PGK was purified from the thermophilic bacterium Thermoanaerobacter sp. Rt8.G4 (optimum growth temperature 68 °C), the mesophile Zymomonas mobilis (optimum growth temperature 32 °C) and a second, unidentified, soil mesophile designated unid A (optimum growth temperature 27 °C). The kinetic behaviour with temperature of each PGK preparation was distinct, despite the conserved nature of the enzyme. The kcat values increased with temperature, but not as rapidly exponentially, as might be expected from the Arrhenius equation. Maximum kcat values were at much higher temperatures than the optimum growth temperatures for the mesophiles, but for the thermophile the temperature of maximum kcat was close to its optimum growth temperature. Km values were in general nearly constant through the lower temperature ranges, but increased substantially as the optimum temperature (highest kcat) was passed. Thermal irreversible denaturation of the PGK proteins was also investigated by measuring loss of activity over time. In a dilute buffer, Arrhenius plots for denaturation were linear, and the calculated apparent energy of activation (Eact) for denaturation for the thermophilic PGK was 600 kJ·mol-1, whereas for the mesophilic enzymes the values were 200-250 kJ·mol-1. In the presence of substrates, a considerable stabilization occurred, and in the case of the Z. mobilis enzyme, the apparent Eact was increased to 480 kJ·mol-1. A theoretical explanation for these observations is presented. Comparing the kinetics data with irreversible denaturation rates determined at relevant temperatures, it was clear that kcat values reached a maximum, and then decreased with higher temperature before irreversible denaturation had any significant influence.
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Jaafar, Nardiah Rizwana, Dene Littler, Travis Beddoe, Jamie Rossjohn, Rosli Md Illias, Nor Muhammad Mahadi, Mukram Mohamed Mackeen, Abdul Munir Abdul Murad, and Farah Diba Abu Bakar. "Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeastGlaciozyma antarcticaPI12." Acta Crystallographica Section F Structural Biology Communications 72, no. 11 (October 27, 2016): 831–39. http://dx.doi.org/10.1107/s2053230x16015612.

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Fuculose-1-phosphate aldolase (FucA) catalyses the reversible cleavage of L-fuculose 1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde. This enzyme from mesophiles and thermophiles has been extensively studied; however, there is no report on this enzyme from a psychrophile. In this study, the gene encoding FucA fromGlaciozyma antarcticaPI12 (GaFucA) was cloned and the enzyme was overexpressed inEscherichia coli, purified and crystallized. The tetrameric structure of GaFucA was determined to 1.34 Å resolution. The overall architecture of GaFucA and its catalytically essential histidine triad are highly conserved among other fuculose aldolases. Comparisons of structural features between GaFucA and its mesophilic and thermophilic homologues revealed that the enzyme has typical psychrophilic attributes, indicated by the presence of a high number of nonpolar residues at the surface and a lower number of arginine residues.
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Dissertations / Theses on the topic "Mesophiles"

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Ermel, Gwennola. "Etudes de bacteriophages virulents de streptocoques lactiques mesophiles." Rennes 1, 1989. http://www.theses.fr/1989REN10121.

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Etude des bacteriophages lytiques des bacteries lactiques. Les analyses morphologiques, de composition proteique et l'homologie adn-adn ont montre que les phages lytiques se repartissent en deux groupes, prolates et isometriques et qu'ils appartiennent a la famille des siphoviridae. L'analyse en microscopie electronique des genomes phagiques a permis d'emettre l'hypothese d'extremites cohesives. L'existence et la nature de celles-ci ont ete mise en evidence par le sequencage d'une partie du genome
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Jaffer, Ashraf. "An investigation into the mechanism of bioleaching of a predominantly-chalcopyrite concentrate with mesophiles." Master's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/5313.

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Includes bibliographical references.
Bioleaching is an established technology for the treatment of refractory gold ores and concentrates. The bioleaching of sulphide minerals is a complex process in which bacterial and chemical oxidation processes occur simultaneously. Recent studies has provided convincing evidence that the bioleaching of pyrite occurs via a two-step mechanism (Boon, 1996).
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Creuly, Catherine. "Selection d'une collection industrielle de streptocoques mesophiles et thermophiles en vue de leurs utilisations en rotation dans les ferments lactiques." Clermont-Ferrand 2, 1987. http://www.theses.fr/1987CLF2D206.

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Cette etude a contribue a creer une collection de streptocoques lactiques: st. Lactis subsp. Cremoris, st. Lactis subsp. Lactis, st. Lactis subsp. Diacetylactis (bacteries mesophiles) et st. Thermophilus. Onze souches de st. Cremoris appartenant a la collection existante ont ete selectionnees parmi 67 bacteries mesophiles apres des tests de lysotypie (sur 59 phages) et de lysogenie (par action de la mitomycine). Les memes tests ont ete realises sur 30 st. Thermophilus et 19 serums phagiques. Mais ces souches semblent posseder des systemes de restriction-modification puissants et sont donc moins sensibles aux phages. Le peu de phages recoltes n'ont pas permis d'etablir des groupes de lysotypie significatifs et les souches testees ne sont pas lysogenes. Afin d'ameliorer la collection, 554 bacteries mesophiles ont ete isolees; identifiees et leur pouvoir acidifiant determine
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MANSOUR, ET-TANI AHMED. "Role des proteases de paroi des bacteries lactiques mesophiles sur la texture et le gout des fromages a pate pressee." Clermont-Ferrand 2, 1989. http://www.theses.fr/1989CLF21192.

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Etude de differents clones (protease+ et protease) isoles de souches de l. Lactis. Ssp. Cremoris ou l. Lactis ssp lactis. Effets de ces clones sur les proprietes sensorielles du fromage. Etude de l'evolution de la composition chimique, jusqu'a l'affinage (acidite, matiere seche, caseine, par exemple). La maitrise de la proportion de variant proteane+ et protease permettent d'envisager de limiter les defauts de fabrication
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Alves, Matheus Bocchini Rodrigues. "Viabilidade de Campylobacter jejuni e microrganismos indicadores em ração de frangos de corte." Universidade Federal de Uberlândia, 2013. https://repositorio.ufu.br/handle/123456789/13100.

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The objective of this study was to evaluate the viability of Campylobacter jejuni and quantify indicator microorganisms (mesophilic bacteria, total coliforms and E. coli) in initial and final broiler feed artificially contaminated with 103 and 105 CFU of C. jejuni per gram of ration, kept at two different temperatures of storage (25 and 370C) and analyzed on four different storage periods 0, 24, 72 and 120 hours. C. jejuni survived throughout the study period and multiplied when inoculated with 103UFC, with the highest counts observed when the feed was kept at a temperature of 370C. Overall, there was a multiplication of mesophilic microorganisms, but the amount of coliforms didn´t increase with time. This work shows that the importance of feed in the epidemiology of C. jejuni in broilers should be better assessed and instigates other studies to verify the possible symbiotic relationship between C. jejuni and mesophilic.
O objetivo desse estudo foi avaliar a viabilidade de Campylobacter jejuni e quantificar microrganismos indicadores (bactérias mesófilas, coliformes totais e E. coli) em rações inicial e final de frangos de corte artificialmente contaminadas com 103 e 105 UFC de C. jejuni por grama de ração, mantidas em duas diferentes temperaturas de armazenamento (25 e 370C) e analisadas em quatro períodos de armazenamento distintos 0, 24, 72 e 120 horas. A C. jejuni sobreviveu durante todo o período avaliado e se multiplicou quando inoculada 103 UFC, sendo observadas as maiores contagens quando a ração foi mantida na temperatura de 370C. Houve multiplicação de microrganismos mesófilos, mas a quantidade de coliformes não aumentou com o tempo. Este trabalho alerta para a necessidade de melhores investigações sobre a importância da ração na epidemiologia da C. jejuni em frangos de corte e a relação mesófilo e Campylobacter.
Mestre em Ciências Veterinárias
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Menezes, Tila de Alcantara. "Avaliação da temperatura de armazenamento e uso de antimicrobianos na qualidade de doses seminais de suínos." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/179696.

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A bacteriospermia pode prejudicar a qualidade das doses de sêmen suíno. Desta forma, a adição de antimicrobianos (ATM) aos diluentes de sêmen é imprescindível para a manutenção da qualidade das doses inseminantes. Contudo, a crescente ocorrência de resistência bacteriana tem impulsionado a redução do uso de ATM na suinocultura. Nesse sentido, o armazenamento das doses inseminantes em baixas temperaturas pode ser uma alternativa para a remoção dos ATM dos diluentes comerciais. Sendo assim, no presente estudo, foram realizados dois experimentos para avaliar a qualidade espermática e a contagem de unidades formadoras de colônias (UFC/mL) de doses de sêmen suíno submetidas a baixas temperaturas de armazenamento, na ausência ou presença de ATM. No experimento 1, as motilidades (total e progressiva) das doses com ATM foram maiores conforme aumentou a temperatura de armazenamento (P<0,01). Nas doses sem ATM, as motilidades foram inferiores nas mantidas a 5 °C do que nas demais (P<0,05). O número de UFC/mL foi menor nas doses sem ATM mantidas a 5 e 10 °C do que a 17 °C (P<0,05), mas não houve diferença entre as temperaturas de armazenamento nas doses com ATM (P>0,05). As integridades de acrossoma e de membrana plasmática não foram afetadas (P>0,05) pelo uso de ATM, mas foram influenciadas pela temperatura de armazenamento (P<0,0001) No experimento 2, os machos foram categorizados em BONS e RUINS de acordo com a motilidade progressiva das doses com ATM armazenadas a 5 °C nas 120 h, sendo investigado o efeito dessas categorias sobre as variáveis estudadas. A motilidade total das doses armazenadas a 17 °C foi superior à das mantidas a 5 °C diluídas sem ATM (P<0,05). Os percentuais de motilidade progressiva e de acrossomas normais foram superiores nas doses mantidas a 17 °C do que nas mantidas a 5 °C, com ou sem ATM (P<0,05). O número de UFC/ml foi maior nas doses diluídas sem ATM do que nas demais (P<0,05). Após a categorização dos machos, as motilidades (total e progressiva) foram maiores nos machos BONS do que nos RUINS (P<0,05), sem diferença significativa (P>0,05) nas integridades (acrossomal e de membrana plasmática). Apesar de a qualidade espermática ter sido afetada negativamente pelas baixas temperaturas, o armazenamento das doses de sêmen suíno a 5 °C é possível, uma vez que foi mantida a viabilidade espermática in vitro, por até 5 dias, acima do nível mínimo considerado adequado para a inseminação artificial. Contudo, o uso de doses sem antimicrobianos ainda precisa de otimização, posto que que as baixas temperaturas de armazenamento reduzem, mas não inibem por completo o crescimento bacteriano.
Bacteriospermia can impair boar semen dose quality. Thus, the addition of antibiotics (ATB) is indispensable for maintaining semen doses quality. Nevertheless, growing bacterial resistance occurrence have had driven to a reduction in use of ATB in pig industry. In this sense, storage of semen doses at low temperature may be an alternative to removal ATB of commercial semen extenders. Therefore, the aim of the present study was to assess sperm quality and number of colony-forming units (CFU mL-1) in boar semen doses stored at low storage temperatures with or without ATB, in two experiments. In experiment 1, in semen doses with ATB, total and progressive motility increased as the storage temperature increased (P<0.01). In semen doses without ATB, total and progressive motility were observed to be lower when stored at 5 °C than at 10 and 17 °C (P<0.05). The number of CFU mL-1 was lower in semen doses without ATB stored at 5 and 10 °C than at 17 °C (P<0.05), but there was no difference among storage temperatures in doses with ATB (P>0.05). Acrosome and sperm membrane integrity were not influenced (P>0.05) by using ATB, but they were influenced by storage temperature (P<0,0001) In experiment 2, boars were grouped in GOOD and POOR according to progressive motility in doses stored for up to 120 h at 5 °C. So, the effect of this classification on assessed variables, was investigated. Total motility was higher in doses stored at 17 °C than in doses without ATB stored at 5 °C (P<0.05). The percentages of progressive motility and normal acrosomes were higher in doses stored at 17 °C than in doses stored at 5 °C, with or without ATB (P<0.05). The number of CFU mL-1 was higher in doses without ATB than in remaining ones (P<0.05). Total and progressive motility were observed to be higher in GOOD than in POOR boars (P<0.05). There was no difference between groups of boars in acrosome and membrane integrity (P>0.05). Despite sperm quality was negatively affected by low temperatures, the storage of boar semen doses at 5 °C is possible, since sperm viability in vitro was maintained for up to 5 days, fulfilling the requirements of semen quality to be used in artificial insemination. Nevertheless, the use of semen doses without ATB will need optimization, since low storage temperatures decreased bacterial growth, but not completely inhibit it.
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Hussy, Ines. "Mesophilic fermentative hydrogen production from biomass." Thesis, University of South Wales, 2005. https://pure.southwales.ac.uk/en/studentthesis/mesophilic-fermentative-hydrogen-production-from-biomass(c099e92e-5777-48e9-bf1f-a1f72d91254e).html.

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Hydrogen is considered a possible alternative to fossil fuels. Hydrogen can be produced through dark fermentation with 1 mol hexose yielding a maximum of 4 mol hydrogen in association with acetate production, and 2 mol hydrogen in association with butyrate production. However, an economically and technically feasible process is yet to be established. So far research into fermentative hydrogen production has focused on pure and soluble carbohydrates, particularly glucose. To reduce substrate costs, use of more complex low-cost co- and waste products of the food industry or biomass crops which have undergone minimum pre-treatment would be desirable. Also, whilst much research to date has focused on use of pure bacterial strains, an easily obtainable mixed microflora would be preferable to avoid costs of substrate sterilisation. Therefore this research project focused on fermentative hydrogen production from three abundant (in the UK) low cost substrates, namely a wheat starch co-product, sugarbeet and perennial ryegrass. Anaerobic digester sludge obtained from the local sewage works was used as inoculum in a continuously stirred tank reactor. Production of hydrogen and other fermentation products was measured to gain information about the main metabolic pathways used. To lower hydrogen partial pressure the reactor was sparged with nitrogen and the effect on hydrogen production observed. It was demonstrated that stable fermentative hydrogen production from the wheat starch co-product and sugarbeet water extract was possible in continuous operation. Hydrogen production from grass extract was demonstrated in batch mode. Sparging with nitrogen significantly increased hydrogen yields, by 46% for the wheat starch co-product, by 67% for sugarbeet water extract, and by 184% for ryegrass extract. Maximum yields achieved were 1.9 mol hydrogen per mol hexose converted for 16 days on starch, 1.7 mol per mol hexose converted for 5 days on sugarbeet water extract and 0.8 mol hydrogen per mol hexose converted in batch from grass extract. Therefore up to 48% of the maximum theoretical hydrogen yield was produced. Various factors were identified as preventing higher hydrogen yields. Hydrogen production was more closely related to butyrate than acetate concentration. Also, lactate, ethanol and propionate, which are products of carbohydrate fermenting metabolic pathways that do not produce hydrogen, were detected, as were signs of hydrogen consuming homoacetogenesis in continuous operation.
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He, Lin. "Studies on xylan depolymerisation by the mesophile Streptomyces A451." Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303667.

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Giovannini, Fabio. "Chirale Synthesebausteine durch enantioselektive Reduktion mit mesophilen und thermophilen Mikroorganismen /." [S.l.] : [s.n.], 1986. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=8004.

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Massanet-Nicolau, Jaime. "Mesophilic fermentative hydrogen production from sewage biosolids." Thesis, University of South Wales, 2009. https://pure.southwales.ac.uk/en/studentthesis/mesophilic-fermentative-hydrogen-production-from-sewage-biosolids(45d910c7-f8d1-4c9d-bc46-ca0b80de8361).html.

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Abstract:
The increasing cost of fossil fuels, combined with concerns about their impact on our environment has led to a renewed interest in hydrogen as a clean, sustainable, alternative energy vector. Using sewage biosolids as the substrate for fermentative hydrogen production offers several advantages over the use of other biomass sources. It is available at little or no cost and is abundant, being produced wherever there are human settlements, with 1.3 million tonnes (dry solids) per year currently being produced in the U.K alone. This research demonstrated the feasibility of hydrogen production from sewage biosolids via anaerobic fermentation. To do this a number of issues specifically relating to the nature of sewage biosolids had to be addressed. Firstly, the solids content and rheology made automatic feeding difficult. The feedstock also contained high levels of indigenous microorganisms and a high ratio of insoluble to soluble carbohydrate. To address these challenges, a novel reactor design using wide bore tubing and computer controlled pumping equipment was successfully used to construct a working continuously fed bio-reactor. A combination of heat treatment at 70°C for one hour and pre-treatment with a commercially available food processing enzyme mixture was found to be the most efficient method of inactivating competing microorganisms and improving substrate quality. Hydrogen was successfully produced via batch fermentation of primary sewage biosolids which had undergone heat treatment and enzymatic digestion. When fermentation took place at pH 5.5 a peak hydrogen production rate of 3.75 cm3 min"1 was observed. At this pH the hydrogen yield was 0.37 mol H2 mol~ : carbohydrate, equivalent to 18.14 L H2 kg"1 dry solids. Fermentative hydrogen production from sewage biosolids was also demonstrated in a five litre, continuously fed bio-reactor for the first time. A comparison of different hydraulic retention times showed that hydrogen production was most stable at a HRT of 24 hours. A hydrogen producing fermenter was successfully linked to a methanogenic bio-reactor in a two stage digestion process.
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Books on the topic "Mesophiles"

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Kugelman, Irwin J. Comparative evaluation of mesophilic and thermophilic anaerobic digestion. Cincinnati, OH: U.S. Environmental Protection Agency, Risk Reduction Engineering Laboratory, 1989.

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Palfrey, R. N. The thermophilic aerobic/mesophilic anaerobic sludge digestion process. Swindon: Foundation for Water Research, 1993.

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Kugelman, Irwin J. Comparative evaluation of mesophilic and thermophilic anaerobic digestion. Cincinnati, OH: U.S. Environmental Protection Agency, Risk Reduction Engineering Laboratory, 1989.

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Ahn, Johng-Hwa. A comparison of mesophilic and thermophilic upflow anaerobic filters. Birmingham: University of Birmingham, 2001.

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Roberts, Ruth. An evaluation of anaerobic thermophilic/mesophilic dual digestion of sludge. Birmingham: University of Birmingham, 1998.

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Levén, Lotta. Anaerobic digestion at mesophilic and thermophilic temperature: With emphasis on degradation of phenols and structures of microbial communities. Uppsala: Swedish University of Agricultural Sciences, 2006.

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Smith, K. Enzymes of L-malate metabolism. Malate dehydrogenase from porcine heart mesophilic bacteria andthermophilic bacteria and malate synthase from thermophilic bacteria. Manchester: UMIST, 1985.

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Dinsdale, Richard Mark. The mesophilic and thermophilic anaerobic digestion of instant coffee waste waters. 1998.

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Morgan-Sagastume, Fernando. Effect of mesophilic-thermophilic temperature transients on aerobic biological treatment of wastewater. 2003.

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Book chapters on the topic "Mesophiles"

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Da Costa, Milton S., and Nuno Empadinhas. "Biosynthesis of the Compatible Solute Mannosylglycerate from Hyperthermophiles to Mesophiles." In Cellular Origin, Life in Extreme Habitats and Astrobiology, 311–25. Dordrecht: Springer Netherlands, 2005. http://dx.doi.org/10.1007/1-4020-3633-7_21.

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Gomez, Felipe. "Mesophile." In Encyclopedia of Astrobiology, 1015. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-11274-4_518.

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Gomez, Felipe. "Mesophile." In Encyclopedia of Astrobiology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27833-4_518-2.

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Gooch, Jan W. "Mesophile." In Encyclopedic Dictionary of Polymers, 906. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14201.

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Gomez, Felipe. "Mesophile." In Encyclopedia of Astrobiology, 1520–21. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-44185-5_518.

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Gooch, Jan W. "Mesophilic Spoilage." In Encyclopedic Dictionary of Polymers, 906. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14202.

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Schiraldi, Chiara, and Mario De Rosa. "Mesophilic Organisms." In Encyclopedia of Membranes, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-40872-4_1610-2.

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Doi, Roy H. "Cellulosomes from Mesophilic Bacteria." In Bioenergy, 97–106. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815547.ch7.

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Conley, Catharine A. "Aerobic Mesophilic Bacterial Spores." In Encyclopedia of Astrobiology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27833-4_35-2.

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Conley, Catharine A. "Aerobic Mesophilic Bacterial Spores." In Encyclopedia of Astrobiology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-642-27833-4_35-3.

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Conference papers on the topic "Mesophiles"

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Ma, Jingwei, Shuizhou Ke, and Yinguang Chen. "Mesophilic Biohydrogen Production from Food Waste." In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.1042.

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Taylor, Todd J. "Discrimination of thermophilic and mesophilic proteins." In 2009 IEEE International Conference on Bioinformatics and Biomedicine Workshop, BIBMW. IEEE, 2009. http://dx.doi.org/10.1109/bibmw.2009.5332120.

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Taylor, Todd J., and Iosif I. Vaisman. "Discrimination and Classification of Thermophilic and Mesophilic Proteins." In 4th International Symposium on Voronoi Diagrams in Science and Engineering (ISVD 2007). IEEE, 2007. http://dx.doi.org/10.1109/isvd.2007.18.

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Ogbonna, Emmanuel C., Rashid Ali, and Georgios Pissanidis. "Simulation model for mesophilic anaerobic digestion heating system." In 2013 International Conference on Renewable Energy Research and Applications (ICRERA). IEEE, 2013. http://dx.doi.org/10.1109/icrera.2013.6749807.

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Ogbonna, Emmanuel C., Rajnish K. Calay, and Mohamad Y. Mustafa. "Nonlinear black-box model for mesophilic anaerobic digestion." In 2016 International Symposium on Small-scale Intelligent Manufacturing Systems (SIMS). IEEE, 2016. http://dx.doi.org/10.1109/sims.2016.7802904.

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Wang, Yi, and Pramod Pandey. "MESOPHILIC ANAEROBIC DIGESTION OF ANIMAL WASTE AND E.COLI REDUCTION." In International Conference on Engineering and Technology Innovations (ICETI). Volkson Press, 2017. http://dx.doi.org/10.26480/iceti.01.2017.169.171.

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Nath, Abhigyan, Radha Chaube, and Subbiah Karthikeyan. "Discrimination of Psychrophilic and Mesophilic Proteins Using Random Forest Algorithm." In 2012 International Conference on Biomedical Engineering and Biotechnology (iCBEB). IEEE, 2012. http://dx.doi.org/10.1109/icbeb.2012.151.

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Hu, Xuehai, Jing-Bo Xia, Xiaohui Niu, Xuan Ma, Chao-Hong Song, and Feng Shi. "Chaos Game Representation for Discriminating Thermophilic from Mesophilic Protein Sequences." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162487.

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Ademola Oyejide ADEBAYO, Bernd - Linke, and Simeon Olatayo JEKAYINFA. "Investigations on Biogas Potential of Maize Stalk under Mesophilic Condition." In 2011 Louisville, Kentucky, August 7 - August 10, 2011. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2011. http://dx.doi.org/10.13031/2013.38513.

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Lim, Y. G., C. Niwa, N. Nagao, and T. Toda. "Mesophilic Anaerobic Digestion as a Post-treatment for Biofouling Blue Mussels." In OCEANS 2007 - Europe. IEEE, 2007. http://dx.doi.org/10.1109/oceanse.2007.4302273.

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