Journal articles: 'MET Matrix'

1

PARK, CHONGWON, and DANIEL TURNER. "When Richard met CG: reference-point and English copy-raising." Language and Cognition 9, no. 3 (October 3, 2016): 473–500. http://dx.doi.org/10.1017/langcog.2016.25.

Full text

Abstract:

abstractThe aim of this paper is to develop a Cognitive Grammar-based analysis of English Copy-raising (CR) constructions such as Richard seems like he is dancing. We argue that the notion of reference-point plays a crucial role in licensing the matrix-subject of the construction. In CR, with the epistemic verbs seem and appear, the matrix-subject functions as a reference-point in relation to the pronominal copy (if a copy exists) in the embedded clause. The aboutness topicality of the matrix-subject in CR is expected, owing to its reference-point property. The epistemic CR construction is acceptable without a pronominal copy if the matrix-subject functions as a reference-point in relation to the complement clause. The same type of analysis is applied to the CR construction with perceptual resemblance (PR) verbs – sound, look, feel, and smell – leading to the conclusion that the strong dichotomy between epistemic and PR verbs is illusory. It is further demonstrated that expletive there-raising in CR is motivated by the same reference-point phenomenon. The difference between there-raising and other CR examples stems from the role of there as a setting subject. Our reference-point-based analysis predicts a metonymic interpretation of the matrix-subject, which we attribute to the connection between reference-point and metonymy.

APA, Harvard, Vancouver, ISO, and other styles

2

朱, 全军. "Recognition of Structural Damping Coefficient of Thin Plate Based on the Frequency Response Matrix Method." Mechanical Engineering and Technology 06, no. 02 (2017): 134–40. http://dx.doi.org/10.12677/met.2017.62019.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Noriega-Guerra, Heydi, and Vanessa Freitas. "Extracellular Matrix Influencing HGF/c-MET Signaling Pathway: Impact on Cancer Progression." International Journal of Molecular Sciences 19, no. 11 (October 24, 2018): 3300. http://dx.doi.org/10.3390/ijms19113300.

Full text

Abstract:

The extracellular matrix (ECM) is a crucial component of the tumor microenvironment involved in numerous cellular processes that contribute to cancer progression. It is acknowledged that tumor–stromal cell communication is driven by a complex and dynamic network of cytokines, growth factors and proteases. Thus, the ECM works as a reservoir for bioactive molecules that modulate tumor cell behavior. The hepatocyte growth factor (HGF) produced by tumor and stromal cells acts as a multifunctional cytokine and activates the c-MET receptor, which is expressed in different tumor cell types. The HGF/c-MET signaling pathway is associated with several cellular processes, such as proliferation, survival, motility, angiogenesis, invasion and metastasis. Moreover, c-MET activation can be promoted by several ECM components, including proteoglycans and glycoproteins that act as bridging molecules and/or signal co-receptors. In contrast, c-MET activation can be inhibited by proteoglycans, matricellular proteins and/or proteases that bind and sequester HGF away from the cell surface. Therefore, understanding the effects of ECM components on HGF and c-MET may provide opportunities for novel therapeutic strategies. Here, we give a short overview of how certain ECM components regulate the distribution and activation of HGF and c-MET.

APA, Harvard, Vancouver, ISO, and other styles

4

Weimar, Iris S., Daphne de Jong, Egbert J. Muller, Toshikazu Nakamura, Joost M.H.H. van Gorp, Gijsbert C. de Gast, and Winald R. Gerritsen. "Hepatocyte Growth Factor/Scatter Factor Promotes Adhesion of Lymphoma Cells to Extracellular Matrix Molecules Via α4β1 and α5β1 Integrins." Blood 89, no. 3 (February 1, 1997): 990–1000. http://dx.doi.org/10.1182/blood.v89.3.990.

Full text

Abstract:

Abstract Hepatocyte growth factor (HGF )/scatter factor (SF ) is the ligand for a tyrosine kinase cell surface receptor encoded by the MET protooncogene (c-MET). HGF/SF can induce proliferation and motility in epithelial cells and promotes invasion of carcinoma cells and NIH3T3 fibroblasts transfected with both HGF/SF and c-MET genes. Our results show that HGF/SF and c-MET also play a role in adhesion and invasion of human lymphoma cells. c-MET mRNA is expressed in hemopoietic cells, such as hemopoietic progenitor cells (CD34+ cells) in bone marrow (BM) and mobilized peripheral blood, immature B cells in cord blood and BM, and germinal center B-centroblasts. In normal peripheral blood B cells, which are c-MET−, c-MET expression was induced by PMA, ConA, HGF/SF, and Epstein-Barr virus (EBV) infection. Using immunohistochemistry, we detected c-MET on the cell surface of large activated centroblasts in lymph nodes from patients with B-non–Hodgkin's lymphoma and Hodgkin's disease. In the latter group, c-MET expression correlated well with the presence of EBV. Because HGF/SF and c-MET promote metastasis of carcinoma cells, we studied the effects of c-MET stimulation by HGF/SF of B-lymphoma cells on properties relevant for metastasis, ie, adhesion, migration, and invasion. HGF/SF stimulated adhesion of the c-MET+ B-cell lines to the extracellular matrix molecules fibronectin (FN) and collagen (CN) in a dose dependent manner. However, adhesion to laminin was not affected by HGF/SF. Adhesion to FN was mediated by β1-integrins α4β1 (VLA4) and α5β1 (VLA5) since blocking antibodies against β1- (CD29), α4- (CD49d), or α5- (CD49e) integrin subunits, completely reversed the effect of HGF/SF. Furthermore, HGF/SF induced adhesion was abrogated by addition of genistein, which blocks protein tyrosine kinases, including c-MET. Addition of HGF/SF resulted in a sixfold increase in migration of c-MET B-lymphoma cells through Matrigel, compared to medium alone. In rat fibroblast cultures, HGF/SF doubled the number of c-MET+ B-lymphoma cells that invaded the fibroblast monolayer. In these adhesion, migration and invasion assays HGF/SF had no effect on c-MET− cell lines. In conclusion, c-MET is expressed or can be induced on immature, activated, and certain malignant B cells. HGF/SF increased adhesion of c-MET+ B-lymphoma cells to FN and CN, mediated via β1-integrins α4β1 and α5β1 , and furthermore promoted migration and invasion.

APA, Harvard, Vancouver, ISO, and other styles

5

Djordjevic, Bogdan, and Nebojsa Dincic. "Classification and approximation of solutions to Sylvester matrix equation." Filomat 33, no. 13 (2019): 4261–80. http://dx.doi.org/10.2298/fil1913261d.

Full text

Abstract:

In this paperwesolve Sylvester matrix equation with infinitely-many solutions and conduct their classification. If the conditions for their existence are not met, we provide a way for their approximation by least-squares minimal-norm method.

APA, Harvard, Vancouver, ISO, and other styles

6

Putri, Alyani Rahma, Nilda Tri Putri, Alizar Hasan, Ikhwan Arief, and Hayati Habibah Abdul Talib. "Halal Assessment Model Design in Bakery Industry." Indonesian Journal of Halal Research 3, no. 2 (August 31, 2021): 56–69. http://dx.doi.org/10.15575/ijhar.v3i2.13000.

Full text

Abstract:

Bakery product is a product with huge business opportunities in the domestic and international halal food market. Bakery companies are demanded to keep the trust of the consumers to the bakery product. The design of the halal assessment model is required to determine the critical point in the bakery-making business process. This assessment tool is intended to understand, know, and determine the critical point of the bakery production process from the halalness degree and is reviewed from all aspects such as the materials’ content, as well as the material acquisition and processing method based on 18 criteria of GMP principles (Good Manufacturing Practices). The halal assessment model designed in this research used QFD (Quality Function Deployment) approach which was integrated into the company’s business process and the halal critical bakery was grouped based on SCOR (Supply Chain Operations Reference) model. Matrix 1 integrated GMP (Good Manufacturing Practices) and business process (BP), matrix 2 integrated GMP (Good Manufacturing Practices), and halal critical bakery (HCB). The results of the design model implementation based on the standards set by the Halal Auditor of LPPOM MUI found that the standard matrix component 1 was met by the company by 47%, while the matrix component 2 was only able to meet the Auditor standards by 34% and the matrix component 3 standards were able to fulfilled by the company by 75%. The fulfillment value of each matrix is influenced by the negative gap that occurs, the negative gap occurs because of the standard criteria in the technical matrix that are not met. The result of this halal assessment model design is expected to help the company in evaluating and controlling critical points found in the business processes.

APA, Harvard, Vancouver, ISO, and other styles

7

Sudhir, Sweta, Darryl Lau, Harsh Wadhwa, Saket Jain, Ankush Chandra, Alan Nguyen, Jordan Spatz, et al. "CSIG-04. ROLE OF c-Met/β1 INTEGRIN COMPLEX IN THE METASTATIC CASCADE." Neuro-Oncology 22, Supplement_2 (November 2020): ii28. http://dx.doi.org/10.1093/neuonc/noaa215.116.

Full text

Abstract:

Abstract Metastases cause 90% of human cancer deaths. The metastatic cascade involves 5 steps: local invasion, intravasation, extravasation, metastatic site colonization, and proliferation. While individual mediators of these processes have been investigated, interactions between these mediators remain less well defined. We previously identified a structural complex between receptor tyrosine kinase c-Met and β1 integrin in metastases that form under certain biological and therapeutic inducers, including bevacizumab. Using novel cell culture and in vivo assays, we found that c-Met/β1 complex induction promotes breast cancer intravasation and adhesion to the vessel wall but does not increase extravasation. These effects may be driven by the ability of the c-Met/β1 complex to increase mesenchymal and stem cell characteristics in breast cancer cells. Multiplex transcriptomic analysis revealed upregulated Wnt and hedgehog pathways after c-Met/β1 complex induction in breast cancer cells. We subsequently used CRISPR to introduce a β1 integrin point mutation that prevented binding to c-Met and led to reduced intravasation, confirming the importance of c-Met/β1 integrin binding for the metastatic cascade. OS2966, a therapeutic B1 integrin blocking antibody, disrupted c-Met/β1 binding as well, and decreased invasion, mesenchymal gene expression, and mesenchymal morphology of breast cancer cells. Bone-seeking breast cancer cells exhibited higher c-Met/β1 complex levels than parental controls and preferentially adhere to tissue-specific matrix. Patient bone metastases demonstrated higher c-Met/β1 complex levels than brain metastases. Thus, our research suggests the c-Met/β1 complex drives breast cancer cell intravasation and preferential affinity for bone tissue-specific matrix. Pharmacological targeting of the complex may prevent metastases, particularly osseous metastases.

APA, Harvard, Vancouver, ISO, and other styles

8

Macpherson, G. L., Thai T. Phan, and Brian W. Stewart. "Direct determination (without chromatographic separation) of lithium isotopes in saline fluids using MC-ICP-MS: establishing limits on water chemistry." Journal of Analytical Atomic Spectrometry 30, no. 7 (2015): 1673–78. http://dx.doi.org/10.1039/c5ja00060b.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Jung, Thorsten, Wolfgang Gross, and Margot Zöller. "CD44v6 Coordinates Tumor Matrix-triggered Motility and Apoptosis Resistance." Journal of Biological Chemistry 286, no. 18 (March 3, 2011): 15862–74. http://dx.doi.org/10.1074/jbc.m110.208421.

Full text

Abstract:

Tumor progression requires a crosstalk with the tumor surrounding, where the tumor matrix plays an essential role. We recently reported that only the matrix delivered by a CD44v6-competent (ASMLwt), but not that of a CD44v6-deficient (ASML-CD44vkd) rat pancreatic adenocarcinoma line supports metastasis formation. We here describe that this matrix provides an important feedback toward the tumor cell and that CD44v6 accounts for orchestrating signals received from the matrix. ASMLwt cells contain more hyaluronan synthase-3 and secrete higher amounts of >50 kDa HA than ASML-CD44vkd cells, which secrete more hyaluronidase. Only the ASMLwt-matrix supports migration and apoptosis resistance, which both can be initiated via CD44v6, c-Met, and α6β4 ligand binding and proceed via FAK, PI3K/Akt, and MAPK activation, respectively. However, c-Met- and α6β4-initiated signaling are strongly augmented by the association with CD44v6 as only very weak effects are observed in CD44v6-deficient cells. The same CD44v6-dependent convergence of motility- and apoptosis resistance-related signals also accounts for human tumor lines. Thus, CD44v6 promotes motility and apoptosis resistance via its involvement in assembling a matrix that, in turn, triggers activation of signaling cascades, which proceeds, independent of the initiating receptor-ligand interaction, in a concerted action via CD44v6.

APA, Harvard, Vancouver, ISO, and other styles

10

Tesfay, Lia, Veronique V. Schulz, Sander B. Frank, Laura E. Lamb, and Cindy K. Miranti. "Receptor tyrosine kinase Met promotes cell survival via kinase-independent maintenance of integrin α3β1." Molecular Biology of the Cell 27, no. 15 (August 2016): 2493–504. http://dx.doi.org/10.1091/mbc.e15-09-0649.

Full text

Abstract:

Matrix adhesion via integrins is required for cell survival. Adhesion of epithelial cells to laminin via integrin α3β1 was previously shown to activate at least two independent survival pathways. First, integrin α3β1 is required for autophagy-induced cell survival after growth factor deprivation. Second, integrin α3β1 independently activates two receptor tyrosine kinases, EGFR and Met, in the absence of ligands. EGFR signaling to Erk promotes survival independently of autophagy. To determine how Met promotes cell survival, we inhibited Met kinase activity or blocked its expression with RNA interference. Loss of Met expression, but not inhibition of Met kinase activity, induced apoptosis by reducing integrin α3β1 levels, activating anoikis, and blocking autophagy. Met was specifically required for the assembly of autophagosomes downstream of LC3II processing. Reexpression of wild-type Met, kinase-dead Met, or integrin α3 was sufficient to rescue death upon removal of endogenous Met. Integrin α3β1 coprecipitated and colocalized with Met in cells. The extracellular and transmembrane domain of Met was required to fully rescue cell death and restore integrin α3 expression. Thus Met promotes survival of laminin-adherent cells by maintaining integrin α3β1 via a kinase-independent mechanism.

APA, Harvard, Vancouver, ISO, and other styles

11

Mhawech-Fauceglia, Paulette, Michelle Afkhami, and Tanja Pejovic. "MET/HGF Signaling Pathway in Ovarian Carcinoma: Clinical Implications and Future Direction." Pathology Research International 2012 (December 25, 2012): 1–7. http://dx.doi.org/10.1155/2012/960327.

Full text

Abstract:

The HGF/MET signaling pathway is abnormal in numerous cancers including ovarian cancer. MET is expressed in 70% of human cancer and it is overexpressed in 30% of ovarian cases and cancer cell lines. The HGF/MET pathway plays a role in the initiation and progression of ovarian cancer through the most distinctive biologic program known as “invasive growth” which is accomplished through a coordinated activation of cell motility, invasiveness, degradation of extracellular matrix, survival, and proliferation. Because of its ubiquitous role in cancer, the MET axis seems to be an attractive target for cancer therapy. Numerous HGF/MET pathway inhibitor compounds are already in use in clinical trials in various solid tumors. In this paper, we will discuss the HGF/MET pathway in ovarian cancer, its clinical significance, and its potential use as a target therapy in the future.

APA, Harvard, Vancouver, ISO, and other styles

12

Wadhwa, Harsh, Darryl Lau, Ankush Chandra, Alan Nguyen, Sumedh Shah, Jordan Spatz, Michael Safaee, et al. "CSIG-10. ROLE OF c-Met/β1 INTEGRIN COMPLEX IN THE ESTABLISHMENT OF BRAIN METASTASES." Neuro-Oncology 21, Supplement_6 (November 2019): vi46. http://dx.doi.org/10.1093/neuonc/noz175.181.

Full text

Abstract:

Abstract INTRODUCTION Metastases cause 90% of human cancer deaths. The metastatic cascade involves five steps: invasion, intravasation, extravasation, colonization, and proliferation. While individual mediators of these processes have been investigated, their interactions remain undefined. We previously demonstrated increased formation of a structural complex between receptor tyrosine kinase c-Met and β1 integrin in metastases compared to primary tumors. We used novel cell culture models and in vivo assays to define the role of this complex in individual steps of the metastatic cascade. METHODS The iDimerize heterodimer system was inserted into MDA-MB-231 breast adenocarcinoma cells, allowing c-Met/β1 heterodimerization induction via A/C heterodimerizer treatment. Scratch assays and novel transwell assay modifications were used to measure migration, invasion, intravasation, and extravasation. Proximity ligation assay was performed to measure c-Met/β1 complex. Nanostring panel was used to transcriptionally profile cells. RESULTS c-Met/β1 complex induction promotes breast cancer invasion (p< 0.001), migration (p< 0.05), intravasation (p< 0.01), and adhesion to the vessel wall (p< 0.01). However, it does not increase extravasation in culture or in vivo. These effects may be driven by the ability of c-Met/β1 to increase mesenchymal character (p< 0.05) and stem cell population (p< 0.001). Nanostring analysis revealed upregulated Wnt and hedgehog pathways after c-Met/β1 complex induction, particularly WNT7B (p< 0.05). OS2966, an antibody preventing c-Met/β1 binding, decreased invasion (p< 0.05), intravasation (p< 0.05), and mesenchymal morphology (p< 0.001) and gene expression (p< 0.001). Brain- and bone-seeking breast cancer cells have higher c-Met/β1 complex than controls (p< 0.05) and preferentially adhere to tissue-specific matrix (p< 0.01). CONCLUSIONS The c-Met/β1 complex drives breast cancer cell intravasation. While extravasation is not affected by the complex, preferential affinity for tissue-specific matrix enables the c-Met/β1 complex to drive breast cancer metastases to brain and bone. Pharmacological targeting of the complex may prevent metastases, particularly to the brain and bone.

APA, Harvard, Vancouver, ISO, and other styles

13

Kellermann, Guillaume, Lyes Boudechiche, Anne Weber, and Michelle Hadchouel. "Increased Engraftment of Hepatic Progenitors After Activation of the Hepatocyte Growth Factor Signaling Pathway by Protein Transduction." Experimental Biology and Medicine 234, no. 9 (September 2009): 1102–8. http://dx.doi.org/10.3181/0901-rm-32.

Full text

Abstract:

Cell transplantation has become a major focus in biomedical research. However, efficient engraftment in solid tissues remains a challenge. Hepatocyte growth factor (HGF) signaling increases survival, proliferation, migration, and invasion of many cell types through Met, its cell surface receptor. Therefore, activation of this signaling pathway may improve the ability of many cells to be transplanted. We constructed a constitutively activated form of Met (Tpr-Met) fused to the protein transduction domain of HIV-TAT to activate the HGF/Met pathway for a few hours following cell injection. Matrix-assisted refolding was used to renature TAT-Tpr-Met protein, which was efficiently delivered into cells and recapitulated several biological functions of Met in vitro. Furthermore, treatment of hepatic progenitors with this molecule for one hour before transplantation significantly improved engraftment efficiency (31% untreated cells, 58% treated cells). These findings suggest that the transient transfer of Tpr-Met may provide a new approach to increase the proportion of successfully engrafted cells.

APA, Harvard, Vancouver, ISO, and other styles

14

Dinh Hoe, Han, Tran Van Huong, Khoa Tran, Nguyen Van Hai, and Nguyen Thi Lien. "TAILORING EVIDENCE-BASED INTERVENTIONS TO REDUCE METHAMPHETAMINE USE AMONG HIV-POSITIVE PATIENTS ON METHADONE IN HO CHI MINH, VIETNAM." International Journal of Advanced Research 9, no. 07 (July 31, 2021): 383–90. http://dx.doi.org/10.21474/ijar01/13137.

Full text

Abstract:

This study evaluated the effectiveness of evidence-based intervention to reduce methamphetamine (MET) use among HIV-positive users in Ho Chi Minh. A total of 236 HIV-positive patients were screened, 71 (30.1%) met the inclusion criteria and 51 (71.8%) were enrolled. Of those enrolled, 49 completed 12 weeks of the intervention, most (?%) of whom used amphetamine-type substances at moderate or higher risk of dependence. The intervention reduced the rate of positive urine MET tests from 54.9% to 12.5%. Participants at intermediate risk for MET (93.1%) were significantly more likely to respond to positive behavior management during the first 6 weeks than participants at high-risk (6.9%, p=0.01). The responder group with positive behavioral management (n=29, 56.8%) received phone/SMS intervention during the latter six weeks of the study and were able to maintain negative urine drug tests. In the non-responder group (n=20), there was no significant difference between the group receiving the Matrix intervention and the group receiving the Matrix intervention in combination with positive behavior management. At the end of the study, 31% of responders and 35% of non-responders had viral loads below the detectable threshold. The results of the study suggest that an evidence-based intervention can reduce the MET use among HIV-positive methadone patients. More studies with larger sample size are needed to better evaluate the effectiveness of interventions as well as their feasibility for widespread adoption.

APA, Harvard, Vancouver, ISO, and other styles

15

Schoedel, Karen E., Valerie Zajac Tyner, Tae-Hyoung Kim, George K. Michalopoulos, and Wendy M. Mars. "HGF, MET, and Matrix-Related Proteases in Hepatocellular Carcinoma, Fibrolamellar Variant, Cirrhotic and Normal Liver." Modern Pathology 16, no. 1 (January 2003): 14–21. http://dx.doi.org/10.1097/01.mp.0000043521.96995.db.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

TERUYA, N., M. KYOTOKU, and H. DIAS. "REAL SOLUTION OF THE COMPLEX RANDOM PHASE APPROXIMATION EQUATION." International Journal of Modern Physics E 02, no. 01 (March 1993): 265–71. http://dx.doi.org/10.1142/s0218301393000091.

Full text

Abstract:

The description of several physical phenomena may require the solution of a complex eigenvalue problem of a non-Hermitian matrix. This problem may be met in the description of some nuclear and plasmon collective excitations using the linear-response theory. It is shown that there exists a related real matrix which satisfies the usual standard real eigenvalue problem whose solution yields directly the solution of the original problem.

APA, Harvard, Vancouver, ISO, and other styles

17

MILLER, J. J., D. F. ACTON, and R. J. ST. ARNAUD. "EVALUATION OF CRITERIA FOR THE CLASSIFICATION OF GLEYSOLIC SOILS IN SASKATCHEWAN." Canadian Journal of Soil Science 69, no. 1 (February 1, 1989): 153–64. http://dx.doi.org/10.4141/cjss89-014.

Full text

Abstract:

Six soils within and adjacent to two willow-ring depressions in hummocky moraine of the Dark Brown soil zone were monitored for seasonal changes in water table levels, redox status, and soil temperature to evaluate color citeria for identifying saturated and reduced soils of the Gleysolic order. Soils in the center of the willow-ring depressions had water table levels close to the soil surface, Ept values of < 100 mV, and were covered by hydrophytic vegetation. Soils near the perimeter of the willow-ring depression had water table levels generally below 1 m, Ept values > 400 mV, and were covered by hydrophytic vegetation. Three of the depressional soils had matrix chromas of 1 in the Ae and/or Bt (Btj) horizons, with prominent rusty mottles, and met the color criteria of Humic Luvic Gleysols. The fourth depressional soil had matrix chromas of 1 in the Btj horizon, but no mottles were present within 50 cm of the soil surface, and met the color criteria of an Orthic Humic Gleysol. Two nondepressional soils, on lower slope positions just outside the willow-ring depressions, exhibited water table levels generally below 1.5 m, had an Ept of > 400 mV, and lacked hydrophytic vegetation. Of these, one soil had matrix chromas of 2 and prominent rusty mottles within 50 cm of the soil surface and met the color criteria of a Rego Humic Gleysol. The other had mottles of low chroma within 1 m of the surface and met the color criteria of a Gleyed Rego Dark Brown. Based on this study, the color criteria are adequate for depressional soils but are inadequate for the classification of soils beyond the willow-ring. Key words: Water table level, redox potential, soil temperature, morphological criteria, gleying

APA, Harvard, Vancouver, ISO, and other styles

18

Mora-Gonzalez, Jose, Isaac J. Pérez-López, Irene Esteban-Cornejo, and Manuel Delgado-Fernández. "A Gamification-Based Intervention Program that Encourages Physical Activity Improves Cardiorespiratory Fitness of College Students: ‘The Matrix rEFvolution Program’." International Journal of Environmental Research and Public Health 17, no. 3 (January 30, 2020): 877. http://dx.doi.org/10.3390/ijerph17030877.

Full text

Abstract:

The aim of the present study was to examine the effects of a gamification-based program on cardiorespiratory fitness (CRF) levels of college students. We divided 112 college students into an intervention group (IG) and a control group (CG). IG college students followed a 15-week gamification-based program, whereas CG followed traditional lectures. CRF was assessed using the 20-meter shuttle-run test. CRF significantly improved after the program in the IG compared to CG (d ≤ 0.94, p < 0.001). Only participants of IG had significant CRF improvements (d ≤ 0.87, p < 0.001) between pre- and post-assessments. In the IG, from the students who attended 100% of lectures, 87.8% met physical activity recommendations for 100% of weeks, whereas from those who attended <100%, only 26.7% met them them for 100% of weeks (p < 0.001). Participants who met recommendations 100% of weeks had a significant CRF improvement (p < 0.001). Motivating college students throughout innovative teaching methods (e.g., gamification) can lead to health improvements.

APA, Harvard, Vancouver, ISO, and other styles

19

Xie, Hongbin, Donald E. Watson, and E. Ray Brown. "Evaluation of Two Compaction Levels for Designing Stone Matrix Asphalt." Transportation Research Record: Journal of the Transportation Research Board 1929, no. 1 (January 2005): 149–56. http://dx.doi.org/10.1177/0361198105192900118.

Full text

Abstract:

Current stone matrix asphalt (SMA) design guidelines list two compaction options to design SMA, 50 blows Marshall or 100 gyrations with the Superpave® gyratory compactor (SGC). However, some states have found that 100 gyrations with the SGC is excessive for their materials. In this study a lower compaction level of 65 gyrations was used to compare with the standard 100 gyrations to design SMA mixtures. Results showed that mixtures designed by 65 gyrations had an average of 0.7% higher optimum asphalt content and 1.5% higher voids in mineral aggregate (VMA) than those designed by 100 gyrations. All mixtures designed by 65 gyrations met the minimum asphalt content and VMA requirements for SMA, whereas only eight of 15 mixtures designed by 100 gyrations met those two requirements. Compaction at 100 gyrations resulted in an additional 0.62% average aggregate breakdown at the critical sieve as compared with 65 gyrations. SMA mixtures designed by 65 gyrations and 100 gyrations had an average asphalt pavement analyzer rut depth of 3.9 mm and 3.1 mm, respectively. Thirteen of 15 mixtures designed by 65 gyrations performed well if 5.0 mm was set as the maximum allowed rut depth. On the basis of this study, 65 gyrations can be used to design a more durable SMA mixture, while still maintaining the good rutting resistance that SMA mixtures are noted for. The successful design by 65 gyrations for all five aggregates in this study indicates that a lower design compaction level may allow the use of more aggregate sources for SMA mixtures.

APA, Harvard, Vancouver, ISO, and other styles

20

Mukai, Shoichiro, Koji Yamasaki, Masato Fujii, Takahiro Nagai, Naoki Terada, Hiroaki Kataoka, and Toshiyuki Kamoto. "Dysregulation of Type II Transmembrane Serine Proteases and Ligand-Dependent Activation of MET in Urological Cancers." International Journal of Molecular Sciences 21, no. 8 (April 11, 2020): 2663. http://dx.doi.org/10.3390/ijms21082663.

Full text

Abstract:

Unlike in normal epithelium, dysregulated overactivation of various proteases have been reported in cancers. Degradation of pericancerous extracellular matrix leading to cancer cell invasion by matrix metalloproteases is well known evidence. On the other hand, several cell-surface proteases, including type II transmembrane serine proteases (TTSPs), also induce progression through activation of growth factors, protease activating receptors and other proteases. Hepatocyte growth factor (HGF) known as a multifunctional growth factor that upregulates cancer cell motility, invasiveness, proliferative, and anti-apoptotic activities through phosphorylation of MET (a specific receptor of HGF). HGF secreted as inactive zymogen (pro-HGF) from cancer associated stromal fibroblasts, and the proteolytic activation by several TTSPs including matriptase and hepsin is required. The activation is strictly regulated by HGF activator inhibitors (HAIs) in physiological condition. However, downregulation is frequently observed in cancers. Indeed, overactivation of MET by upregulation of matriptase and hepsin accompanied by the downregulation of HAIs in urological cancers (prostate cancer, renal cell carcinoma, and bladder cancer) are also reported, a phenomenon observed in cancer cells with malignant phenotype, and correlated with poor prognosis. In this review, we summarized current reports focusing on TTSPs, HAIs, and MET signaling axis in urological cancers.

APA, Harvard, Vancouver, ISO, and other styles

21

Atchonouglo, K., and K. Nwuitcha. "MATRIX STUDY OF THE EQUATION OF SOLID RIGID MOTIONS." Advances in Mathematics: Scientific Journal 10, no. 9 (September 13, 2021): 3195–207. http://dx.doi.org/10.37418/amsj.10.9.9.

Full text

Abstract:

In this article, we described the equations of motion of a rigid solid by a matrix formulation. The matrices contained in our movement description are homogeneous to the same unit. Inertial characteristics are met in a 4x4 positive definite symmetric matrix called "tensor generalized Poinsot." This matrix consists of 3x3 positive definite symmetric matrix called "inertia tensor Poinsot", the coordinates of the center of mass multiplied by the total body mass and the total mass of the rigid body. The equations of motion are formulated as a gender skew 4x4 matrices. They summarize the "principle of fundamental dynamics". The Poinsot generalized tensor appears linearly in this equality as required by the linear dependence of the equations of motion with the ten characteristics inertia of the rigid solid.

APA, Harvard, Vancouver, ISO, and other styles

22

Wilson, Doug, Don Beckley, and Joseph H. Koo. "Development of silicone matrix-based advanced composites for thermal protection." High Performance Polymers 6, no. 2 (April 1994): 165–81. http://dx.doi.org/10.1177/095400839400600207.

Full text

Abstract:

A new class of silicone matrix-based composites has been developed, particularly for use in the thermal protection of missile launch structures. In this application. screening tests have shown that these materials, coded SM8000, offer three-fold performance improvement over conventional phenolic-based composites. Initial investigations designed to identify commercial opportunities for the material have also met with some success. Promising applications as diverse as commercial jet-engine fire shields and high-temperature automotive gaskets have illustrated the potential widespread use of this type of material. This paper will examine the development, properties and applications of these silicone matrix composites.

APA, Harvard, Vancouver, ISO, and other styles

23

Rodríguez-Cantó, Pedro J., Rafael Abargues, Henry Gordillo, Isaac Suárez, Vladimir Chirvony, Sandra Albert, and Juan Martínez-Pastor. "UV-patternable nanocomposite containing CdSe and PbS quantum dots as miniaturized luminescent chemo-sensors." RSC Advances 5, no. 26 (2015): 19874–83. http://dx.doi.org/10.1039/c4ra02812k.

Full text

Abstract:

We have developed a patternable nanocomposite sensor based on luminescent CdSe QDs and a polyisoprene-based photoresist (PIP) as host matrix that showed chemosensing response against MET and EDA in vapour with a LOD around 0.1 pg and 15 ng, respectively.

APA, Harvard, Vancouver, ISO, and other styles

24

Steiner, A. K., G. Kirchengast, and H. P. Ladreiter. "Inversion, error analysis, and validation of GPS/MET occultation data." Annales Geophysicae 17, no. 1 (January 31, 1999): 122–38. http://dx.doi.org/10.1007/s00585-999-0122-5.

Full text

Abstract:

Abstract. The global positioning system meteorology (GPS/MET) experiment was the first practical demonstration of global navigation satellite system (GNSS)-based active limb sounding employing the radio occultation technique. This method measures, as principal observable and with millimetric accuracy, the excess phase path (relative to propagation in vacuum) of GNSS-transmitted radio waves caused by refraction during passage through the Earth's neutral atmosphere and ionosphere in limb geometry. It shows great potential utility for weather and climate system studies in providing an unique combination of global coverage, high vertical resolution and accuracy, long-term stability, and all-weather capability. We first describe our GPS/MET data processing scheme from excess phases via bending angles to the neutral atmospheric parameters refractivity, density, pressure and temperature. Special emphasis is given to ionospheric correction methodology and the inversion of bending angles to refractivities, where we introduce a matrix inversion technique (instead of the usual integral inversion). The matrix technique is shown to lead to identical results as integral inversion but is more directly extendable to inversion by optimal estimation. The quality of GPS/MET-derived profiles is analyzed with an error estimation analysis employing a Monte Carlo technique. We consider statistical errors together with systematic errors due to upper-boundary initialization of the retrieval by a priori bending angles. Perfect initialization and properly smoothed statistical errors allow for better than 1 K temperature retrieval accuracy up to the stratopause. No initialization and statistical errors yield better than 1 K accuracy up to 30 km but less than 3 K accuracy above 40 km. Given imperfect initialization, biases >2 K propagate down to below 30 km height in unfavorable realistic cases. Furthermore, results of a statistical validation of GPS/MET profiles through comparison with atmospheric analyses of the European Centre for Medium-range Weather Forecasts (ECMWF) are presented. The comparisons indicate the high utility of the occultation data in that very good agreement of upper troposphere/lower stratosphere temperature (better than 1.5 K rms, <0.5 K bias) is found for a region (Europe+USA) where the ECMWF analyses are known to be good, but poorer agreement for a region (Southern Pacific) where the analyses are known to be degraded.Key words. Atmospheric composition and structure (pressure; density and temperature), Meteorology and atmospheric dynamics (instruments and techniques), Radio science (remote sensing)

APA, Harvard, Vancouver, ISO, and other styles

25

Naka, Takahiko, Carsten Boltze, Amir Samii, Madjid Samii, Christian Herold, Helmut Ostertag, Yukihide Iwamoto, et al. "Expression of c-MET, low-molecular-weight cytokeratin, matrix metalloproteinases-1 and -2 in spinal chordoma." Histopathology 54, no. 5 (April 2009): 607–13. http://dx.doi.org/10.1111/j.1365-2559.2009.03278.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Al-Toukhy s, Al-Toukhy s. "Particles Matters Accumulation and Anatomical Leaf Properties of Three Tree Species Growing in the Industrial Area in Jeddah, Saudi Arabia." journal of King Abdulaziz University - Meteorology, Environment and Arid Land Agriculture Sciences 26, no. 2 (August 24, 2015): 23–32. http://dx.doi.org/10.4197/met.26-2.3.

Full text

Abstract:

Particles matters accumulation and anatomical leaf properties of Camphor (Cinnamomum camphora), Henna (Lawsonia inermis), and Bougainvillea (Bougainvillea spectabilis) trees growing in the industrial zone in Jeddah - Saudi Arabia and Hada Al-Shame area (control) was done. The leaf properties of all tree species growing in the industrial and control showed that each stoma had a raised edge over the guard cell region. The guard cells appeared more shrunken on the polluted leaves as compared with unpolluted leave. The results indicated that the most deposition particles on leaf surfaces of all tree species were: soot (C) and soil dust with characteristic matrix elements (Si, Al, Mg, Ca, K); fuel oil particles rich in Al, Si, Ca, and Pb; coal ash particles containing C, Al, Si, K, Ca, S; and Pb. As a result, leaves of those plant species may be used as bio-indicators for the assessment of particular matters in the industrial areas.

APA, Harvard, Vancouver, ISO, and other styles

27

Modica, Chiara, Martina Olivero, Francesca Zuppini, Melissa Milan, Cristina Basilico, and Elisa Vigna. "HGF/MET Axis Induces Tumor Secretion of Tenascin-C and Promotes Stromal Rewiring in Pancreatic Cancer." Cancers 13, no. 14 (July 14, 2021): 3519. http://dx.doi.org/10.3390/cancers13143519.

Full text

Abstract:

Pancreatic ductal adenocarcinoma is an aggressive tumor characterized by the presence of an abundant stromal compartment contributing significantly to the malignant phenotype. Pancreatic stellate cells are peculiar fibroblasts present in the stroma and represent the predominant source of extracellular matrix proteins, pro-inflammatory cytokines, and growth factors, including hepatocyte growth factor (HGF). Exploiting a co-culture system of human pancreatic stellate cells and cancer cells, we demonstrated that fibroblast activation was reduced upon HGF/MET axis inhibition. To unveil the signaling pathways sustaining stroma modulation orchestrated by MET activation in the tumor, we analyzed the gene expression profile in pancreatic cancer cells stimulated with HGF and treated with HGF/MET inhibitors. Transcriptome analysis showed that, among all the genes modulated by HGF, a subset of 125 genes was restored to the basal level following treatment with the inhibitors. By examining these genes via ingenuity pathway analysis, tenascin C emerged as a promising candidate linking MET signaling and tumor microenvironment. MET-dependent tenascin C modulation in pancreatic cancer cells was validated at RNA and protein levels both in vitro and in vivo. In conclusion, this work identifies tenascin C as a gene modulated by MET activation, suggesting a role in MET-mediated tumor-stroma interplay occurring during pancreatic tumor progression.

APA, Harvard, Vancouver, ISO, and other styles

28

Dias, Benjamin, and Bart Weimer. "Conversion of Methionine to Thiols by Lactococci, Lactobacilli, and Brevibacteria." Applied and Environmental Microbiology 64, no. 9 (September 1, 1998): 3320–26. http://dx.doi.org/10.1128/aem.64.9.3320-3326.1998.

Full text

Abstract:

ABSTRACT Methanethiol has been strongly associated with desirable Cheddar cheese flavor and can be formed from the degradation of methionine (Met) via a number of microbial enzymes. Methionine γ-lyase is thought to play a major role in the catabolism of Met and generation of methanethiol in several species of bacteria. Other enzymes that have been reported to be capable of producing methanethiol from Met in lactic acid bacteria include cystathionine β-lyase and cystathionine γ-lyase. The objective of this study was to determine the production, stability, and activities of the enzymes involved in methanethiol generation in bacteria associated with cheese making. Lactococci and lactobacilli were observed to contain high levels of enzymes that acted primarily on cystathionine. Enzyme activity was dependent on the concentration of sulfur amino acids in the growth medium. Met aminotransferase activity was detected in all of the lactic acid bacteria tested and α-ketoglutarate was used as the amino group acceptor. In Lactococcus lactis subsp. cremorisS2, Met aminotransferase was repressed with increasing concentrations of Met in the growth medium. While no Met aminotransferase activity was detected in Brevibacterium linens BL2, it possessed high levels of l-methionine γ-lyase that was induced by addition of Met to the growth medium. Met demethiolation activity at pH 5.2 with 4% NaCl was not detected in cell extracts but was detected in whole cells. These data suggest that Met degradation in Cheddar cheese will depend on the organism used in production, the amount of enzyme released during aging, and the amount of Met in the matrix.

APA, Harvard, Vancouver, ISO, and other styles

29

Suthiram, Janine, Thomas Ebenhan, Biljana Marjanovic-Painter, Mike M. Sathekge, and Jan Rijn Zeevaart. "Towards Facile Radiolabeling and Preparation of Gallium-68-/Bismuth-213-DOTA-[Thi8, Met(O2)11]-Substance P for Future Clinical Application: First Experiences." Pharmaceutics 13, no. 9 (August 25, 2021): 1326. http://dx.doi.org/10.3390/pharmaceutics13091326.

Full text

Abstract:

Substance P (SP) is a small peptide commonly known as a preferential endogenous ligand for the transmembrane neurokinin-1 receptor. Nuclear Medicine procedures currently involve radiolabeled SP derivatives in peptide radioligand endotherapy of inoperable glioblastoma. Promising clinical results sparked the demand for facile production strategies for a functionalized 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid-[Thi8, Met(O2)11]-SP to allow for rapid Gallium-68 or Bismuth-213 complexation. Therefore, we provide a simple kit-like radiotracer preparation method that caters for the gallium-68 activity eluted from a SnO2 generator matrix as well as preliminary results on the adaptability to produce [213Bi]Bi-DOTA-[Thi8, Met(O2)11]SP from the same vials containing the same starting material. Following a phase of radioanalysis for complexation of gallium-68 to DOTA-[Thi8, Met(O2)11]SP and assessing the radiolabeling parameters, the vials containing appropriate kit-prototype material were produced in freeze-dried batches. The facile radiolabeling performance was tested and parameters for future human application were calculated to meet the criteria for theranostic loco-regional co-administration of activity doses comprising [68Ga]Ga-DOTA-[Thi8, Met(O2)11]SP mixed with [213Bi]Bi-DOTA-[Thi8, Met(O2)11]SP. [68Ga]Ga-DOTA-[Thi8, Met(O2)11]SP was prepared quantitatively from lyophilized starting material within 25 min providing the required molar activity (18 ± 4 GBq/µmol) and activity concentration (98 ± 24 MBq/mL), radiochemical purity (>95%) and sustained radiolabeling performance (4 months at >95% LE) as well as acceptable product quality (>95% for 120 min). Additionally, vials of the same starting materials were successfully adapted to a labeling strategy available for preparation of [213Bi]Bi-DOTA-[Thi8, Met(O2)11]SP providing sufficient activity for 1–2 human doses. The resultant formulation of [68Ga]Ga-/[213Bi]Bi-DOTA-[Thi8, Met(O2)11]SP activity doses was considered of adequate radiochemical quality for administration. This investigation proposes a simple kit-like formulation of DOTA-[Thi8, Met(O2)11]SP—a first-line investigation into a user friendly, straightforward tracer preparation that would warrant efficient clinical investigations in the future. Quantitative radiolabeling was accomplished for [68Ga]Ga-DOTA-[Thi8, Met(O2)11]SP and [213Bi]Bi-DOTA-[Thi8, Met(O2)11]SP preparations; a key requirement when addressing the specific route of catheter-assisted co-injection directly into the intratumoral cavities.

APA, Harvard, Vancouver, ISO, and other styles

30

Lau, Darryl, Harsh Wadhwa, Alan Nguyen, Ankush Chandra, and Manish Aghi. "BSCI-01. ACTIVATION OF c-Met/β1-INTEGRIN COMPLEX RESULTS IN INCREASE OF MESENCHYMAL GENE EXPRESSION AND STEM CELL POPULATION IN METASTATIC BREAST CANCER TO THE BRAIN AND SPINE." Neuro-Oncology Advances 1, Supplement_1 (August 2019): i1. http://dx.doi.org/10.1093/noajnl/vdz014.000.

Full text

Abstract:

Abstract INTRODUCTION: C-met and β-integrins play a central role in nearly all stages of cancer metastasis. They bind at the cell surface, driving ligand independent co-activation of downstream pathways. Greater complex is seen in metastatic tumors vs. its primary tumor counterparts in patients. The molecular, cellular, and clinical effects of complex formation in metastatic breast cancer are investigated. METHODS: Utilizing variations of the MDA-231 breast cancer cell lines (standard MDA-231, inducible complex formation MDA-231, brain seeking MDA 231, lung seeking MDA 231, and bone seeking MDA-231), in vitro and in vivo studies were performed. Clinical correlates from patient samples were studied. RESULTS: Induction of c-Met/β1 complex promotes breast cancer invasion (p< 0.001), migration (p< 0.05), circulation intravasation (p< 0.01), and adhesion (p< 0.01). These effects may be driven by the increased mesenchymal character (p< 0.05) and larger stem cell population (p< 0.001) caused by inducing c-Met/β1 complex formation. OS2966 (a therapeutic β1 integrin blocking antibody) decreases invasion (p< 0.05), intravasation (p< 0.05), and mesenchymal form factor (p< 0.001) and gene expression (p< 0.001) in MDA-MB-231 cells. Brain- and bone-seeking breast cancer cells have higher c-Met/β1 complex than parental controls and preferentially adhere to tissue-specific matrix (p< 0.01). In intracardiac metastasis models, complex formation resulted in significantly higher metastatic burden and shorter survival times (p< 0.001). qPCR data suggests that complex formation may drive exiting and colonization of cancer cells (micrometastasis) rather than tumor growth. Patient brain and bone metastases demonstrated high β1/c-Met levels. CONCLUSIONS: The c-Met/β1 complex drives intravasation and extravasation of breast cancer cells from the circulation. Preferential affinity for tissue-specific matrix enables the c-Met/β1 complex to drive formation of breast cancer metastases to the brain and bone. Pharmacological and genetic targeting of the complex with agents may provide therapeutic approaches to prevent metastases, particularly to the brain and bone.

APA, Harvard, Vancouver, ISO, and other styles

31

Rozova, Vlada S., Ayad G. Anwer, Anna E. Guller, Hamidreza Aboulkheyr Es, Zahra Khabir, Anastasiya I. Sokolova, Maxim U. Gavrilov, et al. "Machine learning reveals mesenchymal breast carcinoma cell adaptation in response to matrix stiffness." PLOS Computational Biology 17, no. 7 (July 23, 2021): e1009193. http://dx.doi.org/10.1371/journal.pcbi.1009193.

Full text

Abstract:

Epithelial-mesenchymal transition (EMT) and its reverse process, mesenchymal-epithelial transition (MET), are believed to play key roles in facilitating the metastatic cascade. Metastatic lesions often exhibit a similar epithelial-like state to that of the primary tumour, in particular, by forming carcinoma cell clusters via E-cadherin-mediated junctional complexes. However, the factors enabling mesenchymal-like micrometastatic cells to resume growth and reacquire an epithelial phenotype in the target organ microenvironment remain elusive. In this study, we developed a workflow using image-based cell profiling and machine learning to examine morphological, contextual and molecular states of individual breast carcinoma cells (MDA-MB-231). MDA-MB-231 heterogeneous response to the host organ microenvironment was modelled by substrates with controllable stiffness varying from 0.2kPa (soft tissues) to 64kPa (bone tissues). We identified 3 distinct morphological cell types (morphs) varying from compact round-shaped to flattened irregular-shaped cells with lamellipodia, predominantly populating 2-kPa and >16kPa substrates, respectively. These observations were accompanied by significant changes in E-cadherin and vimentin expression. Furthermore, we demonstrate that the bone-mimicking substrate (64kPa) induced multicellular cluster formation accompanied by E-cadherin cell surface localisation. MDA-MB-231 cells responded to different substrate stiffness by morphological adaptation, changes in proliferation rate and cytoskeleton markers, and cluster formation on bone-mimicking substrate. Our results suggest that the stiffest microenvironment can induce MET.

APA, Harvard, Vancouver, ISO, and other styles

32

Jalili, Ali, Neeta Shirvaikar, Sara Ilnitsky, A. Robert Turner, Mariusz Z. Ratajczak, and Anna Janowska-Wieczorek. "G-CSF Induces Expression of Both Hepatocyte Growth Factor (HGF) and Its Receptor (c-Met) in Human Hematopoietic Stem/Progenitor Cells and Mature Myeloid Cells - Novel Evidence That during Mobilization the HGF-c-Met Axis Counterbalances G-CSF-Induced Attenuation of the SDF-1-CXCR4 Axis." Blood 110, no. 11 (November 16, 2007): 2203. http://dx.doi.org/10.1182/blood.v110.11.2203.2203.

Full text

Abstract:

Abstract The stromal-derived factor-1 (SDF-1)-CXCR4 axis plays an important role in stem cell trafficking, and G-CSF- induced mobilization decreases SDF-1 expression in the bone marrow (BM) microenvironment and CXCR4 expression by CD34+ hematopoietic stem/progenitor cells (HSPC). Alternatively, the tyrosine kinase c-Met receptor-HGF axis was recently postulated to play an important role in the trafficking of non-hematopoietic cells; however, our previous research demonstrated that while HGF is an important constituent of the BM microenvironment, c-Met is not expressed by BM-derived steady state HSPC (Br J Haem1997;99:228). Recently we observed that both c-Met and HGF are upregulated during tissue organ/injury in a hypoxia-inducible factor-1α-dependent manner (Circ Res2004;95:1191). To determine whether G-CSF-induced mobilization affects the c-Met-HGF axis in HSPC, we isolated human CD34+ cells from BM and mobilized peripheral blood (mPB), mature myeloid cells and stromal cells and evaluated expression of c-Met and HGF by hematopoietic and BM-derived stromal cells without and after exposure to G-CSF, and the chemotactic responses of steady state and mobilized hematopoietic cells to HGF. We confirmed using RT-PCR and FACS analysis that the c-Met receptor is not expressed by steady-state BM CD34+ cells and mature mononuclear cells (MNC), but to our surprise we found that c-Met is expressed in G-CSF-mobilized CD34+ cells and MNC obtained from leukapheresis products. Supporting this was our finding that mPB but not steady state CD34+ cells responded to HGF stimulation by phosphorylation of MAPKp42/44 and AKT. HGF was found to highly expressed in mPB CD34+ cells and MNC but in steady state BM MNC. Moreover, G-CSF stimulation induced HGF expression in steady state BM MNC and BM-derived fibroblastic and mesenchymal stem cells. Importantly, when we compared c-Met expression on circulating PB leukocytes from patients during early and late stages of G-CSF mobilization we found that it increases in the later stages. When we compared the expression of CXCR4 and c-Met on leukocytes from the leukapheresis product vs circulating PB from the same patient we found that CXCR4 expression is similar but expression of c-Met is higher in the leukapheresis product. HGF was also found to be a strong chemoattractant for mPB leukocytes, but not for steady state leukocytes, and the chemotactic activity of HGF was totally inhibited by a c-Met antagonist. Additionally, we demonstrated that c-Met is incorporated into lipid rafts as shown by confocal microscopy and that G-CSF stimulation increases the secretion of matrix metalloproteinase (MMP)-9 from BM MNC, which was inhibited by the c-Met antagonist. A combination of G-CSF and HGF also upregulated membrane-type (MT)-MMPs such as MT1-MMP and MT6-MMP on leukocytes. Thus we demonstrate for the first time that G-CSF induces expression of functional c-Met and HGF in HSPC and leukocytes, and that the HGF-c-Met axis could play an important role in their mobilization and maintenance of high expression of matrix-degrading enzymes, allowing egress of HSPC from the BM.

APA, Harvard, Vancouver, ISO, and other styles

33

Jeffers, M., S. Rong, and G. F. Vande Woude. "Enhanced tumorigenicity and invasion-metastasis by hepatocyte growth factor/scatter factor-met signalling in human cells concomitant with induction of the urokinase proteolysis network." Molecular and Cellular Biology 16, no. 3 (March 1996): 1115–25. http://dx.doi.org/10.1128/mcb.16.3.1115.

Full text

Abstract:

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic effector of cells expressing the Met tyrosine kinase receptor. Although HGF/SF is synthesized by mesenchymal cells and acts predominantly on epithelial cells, we have recently demonstrated that human sarcoma cell lines often inappropriately express high levels of Met and respond mitogenically to HGF/SF. In the present report we show that HGF/SF-Met signalling in the human leiomyosarcoma cell line SK-LMS-1 enhances its in vivo tumorigenicity, an effect for which the mitogenicity of this signalling pathway is likely to play a role. In addition, we found that HGF/SF-Met signalling dramatically induces the in vitro invasiveness and in vivo metastatic potential of these cells. We have studied the molecular basis by which HGFSF-Met signalling mediates the invasive phenotype. A strong correlation has previously been demonstrated between the activation of the urokinase plasminogen activator (uPA) proteolysis network and the acquisition of the invasive-metastatic phenotype, and we show here that HGF/SF-Met signalling significantly increases the protein levels of both uPA and its cellular receptor in SK-LMS-1 cells. This results in elevated levels of cell-associated uPA and enhanced plasmin-generating ability by these cells. These studies couple HGF/SF-Met signalling to the activation of proteases that mediate dissolution of the extracellular matrix-basement membrane, and important property for cellular invasion-metastasis.

APA, Harvard, Vancouver, ISO, and other styles

34

Corona, Oscar, and Franklin Méndez. "A BRIEF METHOD FOR THE SYNTHESIS AND CHARACTERIZATION OF A HYBRID MATERIAL BASED ON CARBON NANOTUBES AND ALUMINA." Revista de Investigación Talentos 7, no. 2 (December 30, 2020): 84–90. http://dx.doi.org/10.33789/talentos.7.2.138.

Full text

Abstract:

This research is presented in the area of composite materials; whose matrix is alumina with carbon nanotubes. The synthesis of CNT-based materials in alumina is driven by the exceptional chemical and mechanical properties of both materials. For this proposal, critical aspects such as the processing technique, the interfacial mechanisms between the Al2O3matrix and CNTs were revised, without losing the focus on the application of this composite in the structural area and determining the strengthening of the matrix due to the presence of reinforcements. This study deals with the synthesis and characterization of a hybrid material based on multi-walled carbon nanotubes (CNTs) and alumina. A simple and efficient methodology is proposed, in which the composite material CNTs/alumina is prepared from the combination of nanofilaments with an aluminum-rich material. Characterization by MET gave evidence of typical carbon nanotube structures supported on aluminous material. Furthermore, MEB-EDS analysis of hybrid composite material confirmed the presence of carbon nanofilaments embedded in the alumina matrix. This study as an initial phase of the research allowed to identify a simple and novel methodology that will be extended in characterization and determination of structural relationships with processing parameters, and it is considering to perform multiscale modeling to know the mechanisms inherent in the compatibility between the matrix and nanofilaments, and thus the phenomenon of reinforcement

APA, Harvard, Vancouver, ISO, and other styles

35

Kajiwara, Kentaro, Shotaro Yamano, Kazuhiro Aoki, Daisuke Okuzaki, Kunio Matsumoto, and Masato Okada. "CDCP1 promotes compensatory renal growth by integrating Src and Met signaling." Life Science Alliance 4, no. 4 (February 11, 2021): e202000832. http://dx.doi.org/10.26508/lsa.202000832.

Full text

Abstract:

Compensatory growth of organs after loss of their mass and/or function is controlled by hepatocyte growth factor (HGF), but the underlying regulatory mechanisms remain elusive. Here, we show that CUB domain-containing protein 1 (CDCP1) promotes HGF-induced compensatory renal growth. Using canine kidney cells as a model of renal tubules, we found that HGF-induced temporal up-regulation of Src activity and its scaffold protein, CDCP1, and that the ablation of CDCP1 robustly abrogated HGF-induced phenotypic changes, such as morphological changes and cell growth/proliferation. Mechanistic analyses revealed that up-regulated CDCP1 recruits Src into lipid rafts to activate STAT3 associated with the HGF receptor Met, and activated STAT3 induces the expression of matrix metalloproteinases and mitogenic factors. After unilateral nephrectomy in mice, the Met-STAT3 signaling is transiently up-regulated in the renal tubules of the remaining kidney, whereas CDCP1 ablation attenuates regenerative signaling and significantly suppresses compensatory growth. These findings demonstrate that CDCP1 plays a crucial role in controlling compensatory renal growth by focally and temporally integrating Src and Met signaling.

APA, Harvard, Vancouver, ISO, and other styles

36

Khorram, O., T. D. Chuang, and W. J. Pearce. "Long-term effects of maternal undernutrition on offspring carotid artery remodeling: role of miR-29c." Journal of Developmental Origins of Health and Disease 6, no. 4 (May 26, 2015): 342–49. http://dx.doi.org/10.1017/s2040174415001208.

Full text

Abstract:

The purpose of this study was to examine the hypothesis that excess maternal glucocorticoids in response to maternal undernutrition programs the expression of extracellular matrix (ECM) components potentially by miR-29c. We measured the expression of mRNA (qRT-PCR) and protein (Western blot) for collagen 3A1, collagen 4A5 and matrix metalloproteinase 2 (MMP2) in offspring carotid arteries from three groups of dams: 50% food-restricted in latter half of gestation [maternal undernutrition (MUN)], MUN dams who received metyrapone (MET) (500 mg/ml ) in drinking water from day 10 of gestation to term, and control dams fed an ad libitum diet. The expression of miR-29c was significantly decreased at 3 weeks, 3 months and 9 months in MUN carotid arteries, and these decreases in expression were partially blocked by treatment of dams with MET. The expression pattern of ECM genes that are targets of miR-29c correlated with miR-29c expression. Expression of mRNA was increased for elastin (ELN) and MMP2 mRNA in 3-week MUN carotids; in 9-month carotids there were also significant increases in expression of Col3A1 and Col4A5. These changes in mRNA expression of ECM genes at 3 weeks and 9 months were blocked by MET treatment. Similarly, the expression of ELN and MMP2 proteins at 3 weeks were increased in MUN carotids, and by 9 months there were also increases in expression of Col3A1 and Col4A5, which were blocked by MET in MUN carotids. Overall, the results demonstrate a close correlation between expression of miR-29c and the ECM proteins that are its targets thus supporting our central hypothesis.

APA, Harvard, Vancouver, ISO, and other styles

37

Hui, Angela Y., Jalna A. Meens, Colleen Schick, Shawna L. Organ, Hui Qiao, Eric A. Tremblay, Erik Schaeffer, Shashi Uniyal, Bosco M. C. Chan, and Bruce E. Elliott. "Src and FAK mediate cell-matrix adhesion-dependent activation of met during transformation of breast epithelial cells." Journal of Cellular Biochemistry 107, no. 6 (August 15, 2009): 1168–81. http://dx.doi.org/10.1002/jcb.22219.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Zhao, Lan, Yan Wu, Xu-Dong Xie, Yi-Fan Chu, Jin-Qing Li, and Limin Zheng. "c-Met identifies a population of matrix metalloproteinase 9-producing monocytes in peritumoural stroma of hepatocellular carcinoma." Journal of Pathology 237, no. 3 (August 3, 2015): 319–29. http://dx.doi.org/10.1002/path.4578.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Tonomura, Hitoshi, Masateru Nagae, Ryota Takatori, Hidenobu Ishibashi, Tomonori Itsuji, and Kenji Takahashi. "The Potential Role of Hepatocyte Growth Factor in Degenerative Disorders of the Synovial Joint and Spine." International Journal of Molecular Sciences 21, no. 22 (November 18, 2020): 8717. http://dx.doi.org/10.3390/ijms21228717.

Full text

Abstract:

This paper aims to provide a comprehensive review of the changing role of hepatocyte growth factor (HGF) signaling in the healthy and diseased synovial joint and spine. HGF is a multifunctional growth factor that, like its specific receptor c-Met, is widely expressed in several bone and joint tissues. HGF has profound effects on cell survival and proliferation, matrix metabolism, inflammatory response, and neurotrophic action. HGF plays an important role in normal bone and cartilage turnover. Changes in HGF/c-Met have also been linked to pathophysiological changes in degenerative joint diseases, such as osteoarthritis (OA) and intervertebral disc degeneration (IDD). A therapeutic role of HGF has been proposed in the regeneration of osteoarticular tissues. HGF also influences bone remodeling and peripheral nerve activity. Studies aimed at elucidating the changing role of HGF/c-Met signaling in OA and IDD at different pathophysiological stages, and their specific molecular mechanisms are needed. Such studies will contribute to safe and effective HGF/c-Met signaling-based treatments for OA and IDD.

APA, Harvard, Vancouver, ISO, and other styles

40

Higuchi, Toshio, Takuya Orita, Ken Katsuya, Yoshiki Yamasaki, Kiyotaka Akiyama, Huiping Li, Tadashi Yamamoto, Yutaka Saito, and Motonao Nakamura. "MUC20 Suppresses the Hepatocyte Growth Factor-Induced Grb2-Ras Pathway by Binding to a Multifunctional Docking Site of Met." Molecular and Cellular Biology 24, no. 17 (September 1, 2004): 7456–68. http://dx.doi.org/10.1128/mcb.24.17.7456-7468.2004.

Full text

Abstract:

ABSTRACT A cDNA encoding a novel mucin protein, MUC20, was isolated as a gene that is up-regulated in the renal tissues of patients with immunoglobulin A nephropathy. We demonstrate here that the C terminus of MUC20 associates with the multifunctional docking site of Met without ligand activation, preventing Grb2 recruitment to Met and thus attenuating hepatocyte growth factor (HGF)-induced transient extracellular signal-regulated kinase-1 and -2 activation. Production of MUC20 reduced HGF-induced matrix metalloproteinase expression and proliferation, which require the Grb2-Ras pathway, whereas cell scattering, branching morphogenesis, and survival via the Gab1/phosphatidylinositol 3-kinase (PI3K) pathways was not affected. Thus, MUC20 reduces HGF-induced activation of the Grb2-Ras pathway but not the Gab1/PI3K pathways. We further demonstrate that the cytoplasmic domain of MUC20 has the ability to oligomerize and that the oligomerization augments its affinity for Met. Taken together, these results suggest that MUC20 is a novel regulator of the Met signaling cascade which has a role in suppression of the Grb2-Ras pathway.

APA, Harvard, Vancouver, ISO, and other styles

41

Nath, D., N. J. Williamson, R. Jarvis, and G. Murphy. "Shedding of c-Met is regulated by crosstalk between a G-protein coupled receptor and the EGF receptor and is mediated by a TIMP-3 sensitive metalloproteinase." Journal of Cell Science 114, no. 6 (March 15, 2001): 1213–20. http://dx.doi.org/10.1242/jcs.114.6.1213.

Full text

Abstract:

A wide repertoire of transmembrane proteins are proteolytically released from the cell surface by a process known as ‘ectodomain shedding’, under both normal and pathophysiological conditions. Little is known about the physiological mechanisms that regulate this process. As a model system, we have investigated the metalloproteinase-mediated cleavage of the hepatocyte growth factor receptor, Met. We show that epidermal growth factor (EGF) receptor activation, either directly by EGF or indirectly via the G-protein coupled receptor (GPCR) agonist lysophosphatidic acid (LPA), induces cleavage of Met through activation of the Erk MAP kinase signalling cascade. The tyrosine kinase activity of the EGFR was a prerequisite for this stimulation, since treatment of cells with a synthetic inhibitor of this receptor, AG1478, completely abrogated shedding. The metalloproteinase mediating Met cleavage was specifically inhibited by the tissue inhibitor of metalloproteinases (TIMP)-3, but not by TIMP-1 or TIMP-2. Furthermore, the level of Met shedding could be modulated by different cell-matrix interactions. Our results indicate that ectodomain shedding is a highly regulated process that can be stimulated by EGFR signalling pathways and integrin ligation.

APA, Harvard, Vancouver, ISO, and other styles

42

Mirman, R. "Tensors of symmetric and unitary groups." Canadian Journal of Physics 65, no. 2 (February 1, 1987): 193–97. http://dx.doi.org/10.1139/p87-030.

Full text

Abstract:

A realization of unitary-group basis states by multinomials (tensors) that realize symmetric-group representation basis states requires that there be the proper set of numbers of these tensors and that unitary-group transformation properties be obtainable from their labels. That these requirements are met is shown here, allowing the actual construction of the unitary states and matrix elements.

APA, Harvard, Vancouver, ISO, and other styles

43

Nan, Ligang, Tao Qin, Ying Xiao, Weikun Qian, Jie Li, Zheng Wang, Jiguang Ma, Qingyong Ma, and Zheng Wu. "Pancreatic Stellate Cells Facilitate Perineural Invasion of Pancreatic Cancer via HGF/c-Met Pathway." Cell Transplantation 28, no. 9-10 (June 4, 2019): 1289–98. http://dx.doi.org/10.1177/0963689719851772.

Full text

Abstract:

Pancreatic cancer (PC) is a highly lethal cancer that has a strong ability for invasion and metastasis, poor prognosis, and a stubbornly high death rate due to late diagnosis and early metastasis. Therefore, a better understanding of the mechanisms of metastasis should provide novel opportunities for therapeutic purposes. As a route of metastasis in PC, perineural invasion (PNI) occurs frequently; however, the molecular mechanism of PNI is still poorly understood. In this study, we show that the hepatocyte growth factor (HGF)/c-Met pathway plays a vital role in the PNI of PC. We found that HGF promotes PC cell migration and invasion by activating the HGF/c-Met pathway, and enhances the expression of nerve growth factor (NGF) and matrix metalloproteinase-9 (MMP9) in vitro. Furthermore, HGF significantly increased PC cell invasion of the dorsal root ganglia (DRG) and promoted the outgrowth of DRG in cocultured models of PC cells and DRG. In contrast, the capacity for invasion and the phenomenon of PNI in PC cells were reduced when the HGF/c-Met pathway was blocked by siRNA. In conclusion, PSCs facilitate PC cell PNI via the HGF/c-Met pathway. Targeting the HGF/c-Met signaling pathway could be a promising therapeutic strategy for PC.

APA, Harvard, Vancouver, ISO, and other styles

44

KAMBLE, VIKRAM G., SAGAR G. KAMBLE, and RAMESH K. D. "PREDICTION OF FRICTIONAL AND WEAR BEHAVIOR OF ALUMINIUM MATRIX COMPOSITES BY ARTIFICIAL NEURAL NETWORK." Journal of Molecular and Engineering Materials 02, no. 03n04 (September 2014): 1450004. http://dx.doi.org/10.1142/s225123731450004x.

Full text

Abstract:

Modern technologies require materials with unusual combination of properties that cannot be met by conventional metal alloys, ceramic, etc. In our work, we prepared the samples of metal alloys such as Al 356- TiB 2. Processing of samples is done by artificial neural network (ANN) which is one of the promising fields of research in predicting experimental results. In our investigation we worked on grain size analysis, micro hardness, regression analysis, friction test, wear test and microstructure analysis of samples to describe the materials properties of Al 356- TiB 2.

APA, Harvard, Vancouver, ISO, and other styles

45

Amirifar, R., and N. Sadati. "A Low-Order H∞ Controller Design for an Active Suspension System via Linear Matrix Inequalities." Journal of Vibration and Control 10, no. 8 (August 2004): 1181–97. http://dx.doi.org/10.1177/1077546304044617.

Full text

Abstract:

We present an application of a new controller order reduction technique with stability and performance preservation based on linear matrix inequality optimization to an active suspension system. In this technique, the rank of the residue matrix of a proper rational approximation of a high-order H ∞ controller subject to the H ∞-norm of a frequency-weighted error between the approximated controller and high-order H ∞ controller is minimized. However, since solving this matrix rank minimization problem is very difficult, the rank objective function is replaced with the nuclear-norm that can be reduced to a semidefinite program, so that it can be solved efficiently Application to the active suspension system of the Automatic Laboratory of Grenoble provides a fourth-order controller. The experimental results show the control specifications are met to a large extent.

APA, Harvard, Vancouver, ISO, and other styles

46

Shintani, Terumichi, Yoshito Kusuhara, Kei Daizumoto, Tsogt-Ochir Dondoo, Hiroki Yamamoto, Hidehisa Mori, Tomoya Fukawa, et al. "The Involvement of Hepatocyte Growth Factor-MET-Matrix Metalloproteinase 1 Signaling in Bladder Cancer Invasiveness and Proliferation. Effect of the MET Inhibitor, Cabozantinib (XL184), on Bladder Cancer Cells." Urology 101 (March 2017): 169.e7–169.e13. http://dx.doi.org/10.1016/j.urology.2016.12.006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Nie, Xiangrong, Qingwen Wang, and Yang Zhang. "A System of Matrix Equations over the Quaternion Algebra with Applications." Algebra Colloquium 24, no. 02 (April 23, 2017): 233–53. http://dx.doi.org/10.1142/s100538671700013x.

Full text

Abstract:

We in this paper give necessary and sufficient conditions for the existence of the general solution to the system of matrix equations [Formula: see text] and [Formula: see text] over the quaternion algebra ℍ, and present an expression of the general solution to this system when it is solvable. Using the results, we give some necessary and sufficient conditions for the system of matrix equations [Formula: see text] over ℍ to have a reducible solution as well as the representation of such solution to the system when the consistency conditions are met. A numerical example is also given to illustrate our results. As another application, we give the necessary and sufficient conditions for two associated electronic networks to have the same branch current and branch voltage and give the expressions of the same branch current and branch voltage when the conditions are satisfied.

APA, Harvard, Vancouver, ISO, and other styles

48

Eweida, A. M., and M. K. Marei. "Naturally Occurring Extracellular Matrix Scaffolds for Dermal Regeneration: Do They Really Need Cells?" BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/839694.

Full text

Abstract:

The pronounced effect of extracellular matrix (ECM) scaffolds in supporting tissue regeneration is related mainly to their maintained 3D structure and their bioactive components. These decellularized matrix scaffolds could be revitalized before grafting via adding stem cells, fibroblasts, or keratinocytes to promote wound healing. We reviewed the online published literature in the last five years for the studies that performed ECM revitalization and discussed the results of these studies and the related literature. Eighteen articles met the search criteria. Twelve studies included adding cells to acellular dermal matrix (ADM), 3 studies were on small intestinal mucosa (SIS), one study was on urinary bladder matrix (UBM), one study was on amniotic membrane, and one study included both SIS and ADM loaded constructs. We believe that, in chronic and difficult-to-heal wounds, revitalizing the ECM scaffolds would be beneficial to overcome the defective host tissue interaction. This belief still has to be verified by high quality randomised clinical trials, which are still lacking in literature.

APA, Harvard, Vancouver, ISO, and other styles

49

Kumpugdee-Vollrath, Mont, and Mario Helmis. "Controlled Release of Resveratrol and Lignan by Matrix Tableting." Advanced Materials Research 746 (August 2013): 330–36. http://dx.doi.org/10.4028/www.scientific.net/amr.746.330.

Full text

Abstract:

The aim of this research work was to develop the controlled release of two model drugs i.e. water insoluble drug - resveratrol and water soluble drug - lignan by matrix tableting with an eccentric tablet machine. For this purpose different kinds of polymers i.e. Metolose 90 SH-4000® (HMPC), Fetocel RT-N-100® (EC) and Eudragit RLPO® (polymethacrylate) were used. The matrix tablets containing 2 %wt of a model drug which were mixed with 5, 10, 20, 30 and 50 %wt of the polymers mentioned above. In addition, a glidant composed of 1 %wt talc and 1 %wt magnesium stearate as well as a filler Ludipress® were processed. Different physical properties of the powder mixtures (e.g. flowability) and of the tablets (e.g. hardness, uniformity of mass or drug content, drug release, etc.) were determined. Most of the tablets met the physical requirements. If the polymer content got higher the release was slower, which can be confirmed by the lower values of k. The release kinetics were described by three typical mathematic models i.e. biphasic, Noyes-Whitney and KorsmeyerPeppas. The best fitting results were ordered as follows: biphasic > Noyes-Witney > KorsmeyerPeppas.

APA, Harvard, Vancouver, ISO, and other styles

50

Maroun, Christiane R., Monica A. Naujokas, Marina Holgado-Madruga, Albert J. Wong, and Morag Park. "The Tyrosine Phosphatase SHP-2 Is Required for Sustained Activation of Extracellular Signal-Regulated Kinase and Epithelial Morphogenesis Downstream from the Met Receptor Tyrosine Kinase." Molecular and Cellular Biology 20, no. 22 (November 15, 2000): 8513–25. http://dx.doi.org/10.1128/mcb.20.22.8513-8525.2000.

Full text

Abstract:

ABSTRACT Epithelial morphogenesis is critical during development and wound healing, and alterations in this program contribute to neoplasia. Met, the hepatocyte growth factor (HGF) receptor, promotes a morphogenic program in epithelial cell lines in matrix cultures. Previous studies have identified Gab1, the major phosphorylated protein following Met activation, as important for the morphogenic response. Gab1 is a docking protein that couples the Met receptor with multiple signaling proteins, including phosphatidylinositol-3 kinase, phospholipase Cγ, the adapter protein Crk, and the tyrosine specific phosphatase SHP-2. HGF induces sustained phosphorylation of Gab1 and sustained activation of extracellular signal-regulated kinase (Erk) in epithelial Madin-Darby canine kidney cells. In contrast, epidermal growth factor fails to promote a morphogenic program and induces transient Gab1 phosphorylation and Erk activation. To elucidate the Gab1-dependent signals required for epithelial morphogenesis, we undertook a structure-function approach and demonstrate that association of Gab1 with the tyrosine phosphatase SHP-2 is required for sustained Erk activation and for epithelial morphogenesis downstream from the Met receptor. Epithelial cells expressing a Gab1 mutant protein unable to recruit SHP-2 elicit a transient activation of Erk in response to HGF. Moreover, SHP-2 catalytic activity is required, since the expression of a catalytically inactive SHP-2 mutant, C/S, abrogates sustained activation of Erk and epithelial morphogenesis by the Met receptor. These data identify SHP-2 as a positive modulator of Erk activity and epithelial morphogenesis downstream from the Met receptor.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'MET Matrix'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles