Academic literature on the topic 'Metabolic pathway manipulation'

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Journal articles on the topic "Metabolic pathway manipulation"

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Moreno-Sánchez, Rafael, Emma Saavedra, Sara Rodríguez-Enríquez, and Viridiana Olín-Sandoval. "Metabolic Control Analysis: A Tool for Designing Strategies to Manipulate Metabolic Pathways." Journal of Biomedicine and Biotechnology 2008 (2008): 1–30. http://dx.doi.org/10.1155/2008/597913.

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The traditional experimental approaches used for changing the flux or the concentration of a particular metabolite of a metabolic pathway have been mostly based on the inhibition or over-expression of the presumed rate-limiting step. However, the attempts to manipulate a metabolic pathway by following such approach have proved to be unsuccessful. Metabolic Control Analysis (MCA) establishes how to determine, quantitatively, the degree of control that a given enzyme exerts on flux and on the concentration of metabolites, thus substituting the intuitive, qualitative concept of rate limiting step
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Marchesi, Federica, Debora Vignali, Beatrice Manini, Alessandra Rigamonti, and Paolo Monti. "Manipulation of Glucose Availability to Boost Cancer Immunotherapies." Cancers 12, no. 10 (2020): 2940. http://dx.doi.org/10.3390/cancers12102940.

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The orchestration of T cell responses is intimately linked to the execution of metabolic processes, both in homeostasis and disease. In cancer tissues, metabolic alterations that characterize malignant transformation profoundly affect the composition of the immune microenvironment and the accomplishment of an effective anti-tumor response. The growing understanding of the metabolic regulation of immune cell function has shed light on the possibility to manipulate metabolic pathways as a strategy to improve T cell function in cancer. Among others, glucose metabolism through the glycolytic pathw
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TEATHER, RON M. "APPLICATION OF GENE MANIPULATION TO RUMEN MICROFLORA." Canadian Journal of Animal Science 65, no. 3 (1985): 563–74. http://dx.doi.org/10.4141/cjas85-067.

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The potential impact of genetic manipulation of microorganisms has been fundamentally altered by the development of gene cloning and DNA synthesis techniques. Classical microbial biochemistry and genetics provided a rationale and methodology for the development of industrial fermentations in which an existing metabolic pathway of an organism could be exploited, directing a major part of the organism's synthetic capacity toward the production of a desired end product. Strains developed for these purposes, however, are able to survive only under controlled conditions in which competition from no
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Nouwen, Lonneke V., and Bart Everts. "Pathogens MenTORing Macrophages and Dendritic Cells: Manipulation of mTOR and Cellular Metabolism to Promote Immune Escape." Cells 9, no. 1 (2020): 161. http://dx.doi.org/10.3390/cells9010161.

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Myeloid cells, including macrophages and dendritic cells, represent an important first line of defense against infections. Upon recognition of pathogens, these cells undergo a metabolic reprogramming that supports their activation and ability to respond to the invading pathogens. An important metabolic regulator of these cells is mammalian target of rapamycin (mTOR). During infection, pathogens use host metabolic pathways to scavenge host nutrients, as well as target metabolic pathways for subversion of the host immune response that together facilitate pathogen survival. Given the pivotal role
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Hayashi, Shunya, Mutsumi Watanabe, Makoto Kobayashi, Takayuki Tohge, Takashi Hashimoto, and Tsubasa Shoji. "Genetic Manipulation of Transcriptional Regulators Alters Nicotine Biosynthesis in Tobacco." Plant and Cell Physiology 61, no. 6 (2020): 1041–53. http://dx.doi.org/10.1093/pcp/pcaa036.

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Abstract The toxic alkaloid nicotine is produced in the roots of Nicotiana species and primarily accumulates in leaves as a specialized metabolite. A series of metabolic and transport genes involved in the nicotine pathway are coordinately upregulated by a pair of jasmonate-responsive AP2/ERF-family transcription factors, NtERF189 and NtERF199, in the roots of Nicotiana tabacum (tobacco). In this study, we explored the potential of manipulating the expression of these transcriptional regulators to alter nicotine biosynthesis in tobacco. The transient overexpression of NtERF189 led to alkaloid
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Ananga, Anthony, Vasil Georgiev, and Violeta Tsolova. "Manipulation and Engineering of Metabolic and Biosynthetic Pathway of Plant Polyphenols." Current Pharmaceutical Design 19, no. 34 (2013): 6186–206. http://dx.doi.org/10.2174/1381612811319340011.

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Ajaz, Saima, Mark J. McPhail, Keshav K. Singh, et al. "Mitochondrial metabolic manipulation by SARS-CoV-2 in peripheral blood mononuclear cells of patients with COVID-19." American Journal of Physiology-Cell Physiology 320, no. 1 (2021): C57—C65. http://dx.doi.org/10.1152/ajpcell.00426.2020.

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The COVID-19 pandemic has been the primary global health issue since its outbreak in December 2019. Patients with metabolic syndrome suffer from severe complications and a higher mortality rate due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We recently proposed that SARS-CoV-2 can hijack host mitochondrial function and manipulate metabolic pathways for their own advantage. The aim of the current study was to investigate functional mitochondrial changes in live peripheral blood mononuclear cells (PBMCs) from patients with COVID-19 and to decipher the pathways of
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Clark, Teresa J., Longyun Guo, John Morgan, and Jorg Schwender. "Modeling Plant Metabolism: From Network Reconstruction to Mechanistic Models." Annual Review of Plant Biology 71, no. 1 (2020): 303–26. http://dx.doi.org/10.1146/annurev-arplant-050718-100221.

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Mathematical modeling of plant metabolism enables the plant science community to understand the organization of plant metabolism, obtain quantitative insights into metabolic functions, and derive engineering strategies for manipulation of metabolism. Among the various modeling approaches, metabolic pathway analysis can dissect the basic functional modes of subsections of core metabolism, such as photorespiration, and reveal how classical definitions of metabolic pathways have overlapping functionality. In the many studies using constraint-based modeling in plants, numerous computational tools
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Ramos, J., A. Wasserfallen, K. Rose, and K. Timmis. "Redesigning metabolic routes: manipulation of TOL plasmid pathway for catabolism of alkylbenzoates." Science 235, no. 4788 (1987): 593–96. http://dx.doi.org/10.1126/science.3468623.

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Fornazier, Ricardo Francisco, Ricardo Antunes Azevedo, Renato Rodrigues Ferreira, and Vanderlei Aparecido Varisi. "Lysine catabolism: flow, metabolic role and regulation." Brazilian Journal of Plant Physiology 15, no. 1 (2003): 9–18. http://dx.doi.org/10.1590/s1677-04202003000100002.

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Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent r
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Dissertations / Theses on the topic "Metabolic pathway manipulation"

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zuller, charles d. "MANIPULATION OF MICROBIAL METABOLIC PATHWAYS FOR THE REDUCTION OF SULFIDE PRODUCTION." MSSTATE, 2003. http://sun.library.msstate.edu/ETD-db/theses/available/etd-03252003-142544/.

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A bacterial additives treatment experiment was conducted to assess the microbial and biochemical changes in stored swine manure. Nitrate salt was added to a slurry of swine waste collected from a waste storage pit to identify the effects of varying levels of nitrate upon the microbial community and the resulting metabolic changes. This research was an attempt to reduce the formation of odorous sulfur-containing compounds and to increase the formation of odorless nitrogen gas by manipulating the metabolic pathways in anaerobic decomposition of the organic matter within manure. Sulfide productio
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Makaula, Siyanda S. S. "Preconditioning and augmented preconditioning via manipulation of metabolic and signalling pathways in the rat heart." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/2789.

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Bibliographical references.<br>Cardiac ischaemic preconditioning (IPC) describes a biological phenomenon whereby a short ischaemic stimulus confers protection to the heart against subsequent prolonged ischaemia and reperfusion injury. Understanding this survival programme will enable us to augment tissue tolerance against cell death. Ischaemic preconditioning is poorly understood, however, certain metabolic events and activation intracellular signalling events are known to trigger this cardioprotection. The purpose of this study was to investigate the metabolic and intracellular signalling eve
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Merali, Zara. "Genetic manipulation of the phenylpropanoid pathway : an investigation into the interface between phenylpropanoid metabolism, cell wall and tissue structure." Thesis, University of East Anglia, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405906.

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Cai, Guiqin. "Understanding the regulation of the metabolic network associated with fermentative hydrogen production in Clostridium butyricum." Thesis, 2012. http://hdl.handle.net/2440/79589.

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Hydrogen is an environmentally friendly and high energy caffier that could be a potential replacement for depleting fossil fuels. Fermentative hydrogen production (FHP) has received great interest in recent decades, as it offers a potential means of producing H₂ from a variety of renewable and waste resources via a low energy process. However, the commercial application of the FHP process has been hampered by its low yields. It becomes important to obtain a better understanding of metabolic pathways and their regulation in H₂-producing microorganisms. The aim of this work was to improve fundam
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"Stimulation of pentose phosphate pathway activity by salinity and dietary manipulations of silver sea bream, sparus sarba." 2004. http://library.cuhk.edu.hk/record=b5892173.

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Leung Ling Yan.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2004.<br>Includes bibliographical references (leaves 117-138).<br>Abstracts in English and Chinese.<br>Abstract (English) --- p.ii<br>Abstract (Chinese) --- p.iv<br>Acknowledgements --- p.vi<br>List of Abbreviation --- p.vii<br>List of Figures and Tables --- p.viii<br>Chapter Chapter 1 --- General Introduction --- p.1<br>Chapter Chapter 2 --- The effect of salinity on pentose phosphate pathway activity and red blood cells resistance to oxidative stress in silver seabream (Sparus sarba) --- p.4<br>Abstract --- p.7<br>
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CASILI, GIOVANNA. "The in vivo pharmacological manipulation of the Nrf-2 pathway and its therapeutic significance in brain diseases, with a possible implication in H2S-regulated lipidic metabolism." Doctoral thesis, 2019. http://hdl.handle.net/11570/3146673.

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Nuclear factor erythroid 2-related factor 2 (Nrf2), a redox-sensitive transcription factor, plays a critical role in the regulation of cellular defence and contributes to a number of cellular processes. Nrf2 is regulated through an interplay of complex transcriptional and post-translational mechanisms that modulates its activity during cellular perturbations or other biological processes thereby ensuring cellular homeostasis is maintained through the orchestration of adaptive responses. Therefore, an ability to modulate the activity of the Nrf2 pathway holds promise as a therapeutic strategy i
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Books on the topic "Metabolic pathway manipulation"

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Stafstrom, Carl E. Dietary Therapy for Neurological Disorders. Edited by Jong M. Rho. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190497996.003.0018.

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Dietary and metabolic therapies such as the high-fat, low-carbohydrate ketogenic diet (KD) are best known for the treatment of intractable epilepsy. Yet, dietary and metabolic approaches have also found some efficacy in a wide variety of other neurological diseases, including autism spectrum disorder, brain trauma, Alzheimer’s disease, sleep disorders, brain tumors, pain, and multiple sclerosis, as discussed in other chapters of this volume. This chapter provides an overview of clinical and experimental studies using the KD in an array of other neurologic disorders: amyotrophic lateral scleros
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Nielsen, Niklas, and David B. Seder. Non-pharmacological neuroprotection in the ICU. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199600830.003.0230.

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After control of the primary process causing acute neurological injury, further control of secondary injury pathways can be achieved by manipulating brain temperature, and achieving biochemical and metabolic homeostasis. Surgical techniques are routinely used to remove blood or trapped cerebrospinal fluid, control mass effect, or repair unstable vascular abnormalities. Therapeutic temperature management to a defined target can be achieved and maintained using cold fluids, ice packs, body surface cooling pads, and surface and intravascular devices with servo (feedback) mechanisms. Successful te
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Bentham, James R. The genetics of congenital heart disease. Edited by José Maria Pérez-Pomares, Robert G. Kelly, Maurice van den Hoff, et al. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780198757269.003.0022.

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Congenital heart disease (CHD) is defined as a structural cardiac malformation resulting from an abnormality of development; 8% of CHD is inherited in a Mendelian fashion and 12% results from chromosomal imbalance. Recurrence risk and new research suggest that even the remaining 80% of patients without an identifiable familial or syndromic basis for disease may have an identifiable genetic cause. The potential to understand these mechanisms is increasing with the advent of new sequencing techniques which have identified multiple or single rare variants and/or copy number variants clustering in
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Book chapters on the topic "Metabolic pathway manipulation"

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Anderson, Nickolas A., and Clint Chapple. "Perturbing Lignin Biosynthesis: Metabolic Changes in Response to Manipulation of the Phenylpropanoid Pathway." In Recent Advances in Polyphenol Research. John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118329634.ch2.

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Bor, Melike, and Filiz Özdemir. "Manipulating Metabolic Pathways for Development of Salt-Tolerant Crops." In Salinity Responses and Tolerance in Plants, Volume 1. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-75671-4_9.

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McCown, Brent H., Eric L. Zeldin, Hamilton A. Pinkalla, and Richard R. Dedolph. "Nodule Culture: A Developmental Pathway with High Potential for Regeneration, Automated Micropropagation, and Plant Metabolite Production from Woody Plants." In Genetic Manipulation of Woody Plants. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1661-9_9.

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Georgiev, Vasil, and Atanas Pavlov. "Genetic Engineering and Manipulation of Metabolite Pathways in Salvia Spp." In Salvia Biotechnology. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-73900-7_10.

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Arun, Muthukrishnan, Jayachandran Halka, and Kumaresan Kowsalya. "CRISPR/Cas9: A Novel Genetic Tool to Manipulate Plant Secondary Metabolite Pathways." In Genetic Manipulation of Secondary Metabolites in Medicinal Plant. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-4939-7_3.

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Oakley, C. Elizabeth, Heather Edgerton-Morgan, and Berl R. Oakley. "Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans." In Fungal Secondary Metabolism. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-122-6_10.

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Holden, M. "Prospects for the Genetic Manipulation of Metabolic Pathways Leading to Secondary Products." In The Impact of Biotechnology on Agriculture. Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0587-0_25.

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Ambrin, Ghazala, and Rashke Eram. "Manipulation of Metabolic Pathways in Regulation and Development of Nutritionally Enriched Crops under Abiotic Stress Factors." In Optimizing Plant Health under Abiotic-Stress Environments. CRC Press, 2025. https://doi.org/10.1201/9781032630380-13.

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Rieger, Don. "Metabolic Pathway Activity." In A Laboratory Guide To The Mammalian Embryo. Oxford University PressNew York, NY, 2004. http://dx.doi.org/10.1093/oso/9780195142266.003.0009.

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Abstract The early development of the mammalian embryo is directed by its inherent genetic programming and affected by its environment. Both the genetic program and the environment act as stimuli to the early embryo to induce developmental responses, initially in its intracellular biochemical activity, and ultimately in cell division, differentiation, and function. Techniques for manipulating gene expression and development, such as gene transfer, cloning, IVF, ICSI, and culture, similarly act as stimuli to induce the desired biochemical and developmental responses.
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Halford, Nigel G. "SNF1-related protein kinase genes in cereals." In Seed Storage Compounds. Oxford University PressOxford, 1990. http://dx.doi.org/10.1093/oso/9780198577683.003.0019.

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Abstract Considerable advances have been made in the study of the regulation of plant gene expression in response to stimuli such as light, temperature, the action of pathogens, and developmental signals. However, relatively little is known about the effects of metabolite levels on the activation of genes encoding enzymes, including those involved in the pathways leading to the accumulation of storage products. Evidence is beginning to emerge that complicated signal transduction pathways exist to enable the plant cell to respond to its metabolic status by activating or inactivating gene expres
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Conference papers on the topic "Metabolic pathway manipulation"

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Balan, Ion, Dinu Gavojdian, Alexandr Budevici, et al. "Proprietățile celulelor stem spermatogoniale în mediul nișelor fiziologice și metabolice testiculare." In "Fiziologia şi sănătatea", congresul fiziologilor. Bulletin of the Academy of Sciences of Moldova. Medical Sciences, 2025. https://doi.org/10.52692/cfzl2024.05.

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Spermatogenesis is a continuous process, maintained by the self-renewal and differentiation of spermatogonial stem cells (SSC), which arise from undifferentiated precursors (gonocytes) in the physiological conditions of the testicular cell niche. SSC serve as the foundation for the physiology of continuous spermatogenesis, they have the ability to self-renew by differentiation to produce spermatozoa suitable for fertilization, therefore the knowledge of the regulatory processes of SSC is of great importance for understanding the fundamental molecular mechanisms of the function of these cells i
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SALAMONSEN, WAYNE, KEVIN YEE CHUEN MOK, PRASANNA KOLATKAR, and S. SUBBIAH. "BioJAKE: A TOOL FOR THE CREATION, VISUALIZATION AND MANIPULATION OF METABOLIC PATHWAYS." In Proceedings of the Pacific Symposium. WORLD SCIENTIFIC, 1998. http://dx.doi.org/10.1142/9789814447300_0038.

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Reports on the topic "Metabolic pathway manipulation"

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Eyal, Yoram, Gloria Moore, and Efraim Lewinsohn. Study and Manipulation of the Flavanoid Biosynthetic Pathway in Citrus for Flavor Engineering and Seedless Fruit. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7570547.bard.

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The proposal was aimed to identify and functionally characterize key genes/enzymes in the citrus flavanone neohesperidoside biosynthetic pathway and to use them as tools for metabolic engineering to decrease bitterness levels in grapefruit. The proposed section on fruit seediness was dropped as suggested by the reviewers of the proposal. Citrus flavor and aroma is composed of complex combinations of soluble and volatile compounds. The former includes mainly sugars, acids and flavanones, a subgroup of flavonoids that includes bitter compounds responsible for the bitter flavor of grapefruit and
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Galili, Gad, Harry J. Klee, and Asaph Aharoni. Elucidating the impact of enhanced conversion of primary to secondary metabolism on phenylpropanoids secondary metabolites associated with flavor, aroma and health in tomato fruits. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597920.bard.

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• Targeted manipulating Phenylalanine (Phe) synthesis is one of the most powerful strategies to boost the biologically and economically important secondary metabolites, including phenylpropaniods, aromatic volatiles and specialized secondary metabolites. • Over-expression of the petunia MYB transcript factor, ODORANT1 (ODO1), results in significant alterations of the levels of specific phenylpropanoid compounds in plants. • Our previous studies indicated that ectopic expression of the feedback-insensitive AroG could break the bottleneck between primary and secondary metabolisms in tomato, ther
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Stern, David, and Gadi Schuster. Manipulating Chloroplast Gene Expression: A Genetic and Mechanistic Analysis of Processes that Control RNA Stability. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7586541.bard.

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New potential for engineering chloroplasts to express novel traits has stimulated research into relevant techniques and genetic processes, including plastid transformation and gene regulation. This BARD-funded research dealt with the mechanisms that influence chloroplast RNA accumulation, and thus gene expression. Previous work on cpRNA catabolism has elucidated a pathway initiated by endonucleolytic cleavage, followed by polyadenylation and exonucleolytic degradation. A major player in this process is the nucleus-encoded exoribo-nuclease/polymerase polynucleotide phosphorylase (PNPase). Bioch
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Fridman, Eyal, and Eran Pichersky. Tomato Natural Insecticides: Elucidation of the Complex Pathway of Methylketone Biosynthesis. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7696543.bard.

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Plant species synthesize a multitude of specialized compounds 10 help ward off pests. and these in turn may well serve as an alternative to synthetic pesticides to reduce environmental damage and health risks to humans. The general goal of this research was to perform a genetic and biochemical dissection of the natural-insecticides methylketone pathway that is specific to the glandular trichomes of the wild species of tomato, Solanumhabrochaites f. glabratum (accession PI126449). Previous study conducted by us have demonstrated that these compounds are synthesized de novo as a derivate pathway
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Or, Etti, David Galbraith, and Anne Fennell. Exploring mechanisms involved in grape bud dormancy: Large-scale analysis of expression reprogramming following controlled dormancy induction and dormancy release. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7587232.bard.

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The timing of dormancy induction and release is very important to the economic production of table grape. Advances in manipulation of dormancy induction and dormancy release are dependent on the establishment of a comprehensive understanding of biological mechanisms involved in bud dormancy. To gain insight into these mechanisms we initiated the research that had two main objectives: A. Analyzing the expression profiles of large subsets of genes, following controlled dormancy induction and dormancy release, and assessing the role of known metabolic pathways, known regulatory genes and novel se
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Brown Horowitz, Sigal, Eric L. Davis, and Axel Elling. Dissecting interactions between root-knot nematode effectors and lipid signaling involved in plant defense. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7598167.bard.

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Root-knot nematodes, Meloidogynespp., are extremely destructive pathogens with a cosmopolitan distribution and a host range that affects most crops. Safety and environmental concerns related to the toxicity of nematicides along with a lack of natural resistance sources threaten most crops in Israel and the U.S. This emphasizes the need to identify genes and signal mechanisms that could provide novel nematode control tactics and resistance breeding targets. The sedentary root-knot nematode (RKN) Meloidogynespp. secrete effectors in a spatial and temporal manner to interfere with and mimic multi
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