Log in

Relevant bibliographies by topics / Metabolite extraction / Journal articles

To see the other types of publications on this topic, follow the link: Metabolite extraction.

Journal articles on the topic 'Metabolite extraction'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Metabolite extraction.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Xiong, Wenzheng, Florian Zirpel, M. Zameel Cader, Daniel C. Anthony, and Fay Probert. "Extraction Methods for Brain Biopsy NMR Metabolomics: Balancing Metabolite Stability and Protein Precipitation." Metabolites 14, no. 11 (2024): 609. http://dx.doi.org/10.3390/metabo14110609.

Full text

Abstract:

Background/Objectives: Metabolic profiling of tissue samples via liquid-state nuclear magnetic resonance (NMR) requires the extraction of polar metabolites in a suitable deuterated solvent. Such methods often prioritise metabolite recovery over protein removal due to the relatively low sensitivity of NMR metabolomics and the routine use of methods able to supress residual protein signals. However, residual protein may impact metabolite integrity and the metabolite stability after NMR sample preparation is often overlooked. This study aimed to investigate the effect of residual protein contamin

APA, Harvard, Vancouver, ISO, and other styles

2

Tong, Shi-Ruo, Ting-Hun Lee, Soon-Keng Cheong, and Yang-Mooi Lim. "Untargeted metabolite profiling on the water-soluble metabolites of edible bird's nest through liquid chromatography-mass spectrometry." February-2020 13, no. 2 (2020): 304–16. http://dx.doi.org/10.14202/vetworld.2020.304-316.

Full text

Abstract:

Background and Aim: Edible bird's nest (EBN) is the nutrient-rich salivary bioproduct produced by swiftlets in Southeast Asia. Currently, researchers are exploring the therapeutic effects of EBN, such as cell growth promotion, antioxidant content, antiviral effects, bone strengthening, eyes care, and neuroprotection bioactivities. The therapeutic effects of EBN have been studied through different extraction methods but the metabolites profile of the EBN in each extract has not yet been elucidated. This study aimed to profile the water-soluble metabolites of EBN prepared in different extraction

APA, Harvard, Vancouver, ISO, and other styles

3

Gines, Brandon, Willard Collier, Mohamed Abdalla, and Teshome Yehualaeshet. "Influence of Metabolite Extraction Methods on 1H-NMR-Based Metabolomic Profiling of Enteropathogenic Yersinia." Methods and Protocols 1, no. 4 (2018): 45. http://dx.doi.org/10.3390/mps1040045.

Full text

Abstract:

Metabolite extraction is one of the critical steps in microbial metabolome analysis. It affects both the observed metabolite content and biological interpretation of the data. Several methods exist for metabolite extraction of microbes, but the literature is not consistent regarding the sample model, adequacy, and performance of each method. In this study, an optimal extraction protocol for Yersinia intracellular metabolites was investigated. The effect of five extraction protocols consisting of different extraction solvent systems (60% methanol, 100% methanol, acetonitrile/methanol/water (2:2

APA, Harvard, Vancouver, ISO, and other styles

4

Dias, Daniela B., Raphaela Fritsche-Guenther, Friederike Gutmann, Georg N. Duda, Jennifer Kirwan, and Patrina S. P. Poh. "A Comparison of Solvent-Based Extraction Methods to Assess the Central Carbon Metabolites in Mouse Bone and Muscle." Metabolites 12, no. 5 (2022): 453. http://dx.doi.org/10.3390/metabo12050453.

Full text

Abstract:

The identification of endogenous metabolites has great potential for understanding the underlying tissue processes occurring in either a homeostatic or a diseased state. The application of gas chromatography-mass spectrometry (GC-MS)-based metabolomics on musculoskeletal tissue samples has gained traction. However, limited comparison studies exist evaluating the sensitivity, reproducibility, and robustness of the various existing extraction protocols for musculoskeletal tissues. Here, we evaluated polar metabolite extraction from bone and muscle of mouse origin. The extraction methods compared

APA, Harvard, Vancouver, ISO, and other styles

5

Jin, Zhaoxia, Ruyi Wan, Ruxue Yan, et al. "Microwave-Assisted Extraction of Multiple Trace Levels of Intermediate Metabolites for Camptothecin Biosynthesis in Camptotheca acuminata and Their Simultaneous Determination by HPLC-LTQ-Orbitrap-MS/MS and HPLC-TSQ-MS." Molecules 24, no. 4 (2019): 815. http://dx.doi.org/10.3390/molecules24040815.

Full text

Abstract:

Camptothecin (CPT) has strong antitumor activity and is used as an anticancer therapeutic agent. To better understand and decipher the pathway of CPT biosynthesis in Camptotheca acuminata, the main purpose here was focused on creating an effective extraction strategy for a rich intermediate metabolite profile. In the present study, a 70% aqueous acetonitrile was verified as an optimal extraction solvent for microwave-assisted extraction (MAE) of metabolites by spiking experiments. Based on multi-objective optimization, the best extraction conditions of a solid-liquid ratio of 1:20, microwave p

APA, Harvard, Vancouver, ISO, and other styles

6

Khoza, B. S., L. Chimuka, E. Mukwevho, P. A. Steenkamp, and N. E. Madala. "The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA." Evidence-Based Complementary and Alternative Medicine 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/914759.

Full text

Abstract:

Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial. In the current study, an organic solvent-free method, namely, pressurised hot water extraction (PHWE), was used to extract pharmacologically important metabolites from driedMoringa oleiferaleaves. Here, the temperature of the extraction solvent (pure water) was altered while keeping other factors constant using a homemade PHWE system. Samples extracted at different temperatures (50, 100, and 150°C) were a

APA, Harvard, Vancouver, ISO, and other styles

7

Woodward, Alison, Alina Pandele, Salah Abdelrazig, et al. "Integrated Metabolomics and Transcriptomics Using an Optimised Dual Extraction Process to Study Human Brain Cancer Cells and Tissues." Metabolites 11, no. 4 (2021): 240. http://dx.doi.org/10.3390/metabo11040240.

Full text

Abstract:

The integration of untargeted metabolomics and transcriptomics from the same population of cells or tissue enhances the confidence in the identified metabolic pathways and understanding of the enzyme–metabolite relationship. Here, we optimised a simultaneous extraction method of metabolites/lipids and RNA from ependymoma cells (BXD-1425). Relative to established RNA (mirVana kit) or metabolite (sequential solvent addition and shaking) single extraction methods, four dual-extraction techniques were evaluated and compared (methanol:water:chloroform ratios): cryomill/mirVana (1:1:2); cryomill-was

APA, Harvard, Vancouver, ISO, and other styles

8

Altenbuchinger, Michael, Henry Berndt, Robin Kosch, et al. "Bucket Fuser: Statistical Signal Extraction for 1D 1H NMR Metabolomic Data." Metabolites 12, no. 9 (2022): 812. http://dx.doi.org/10.3390/metabo12090812.

Full text

Abstract:

Untargeted metabolomics is a promising tool for identifying novel disease biomarkers and unraveling underlying pathomechanisms. Nuclear magnetic resonance (NMR) spectroscopy is particularly suited for large-scale untargeted metabolomics studies due to its high reproducibility and cost effectiveness. Here, one-dimensional (1D) 1H NMR experiments offer good sensitivity at reasonable measurement times. Their subsequent data analysis requires sophisticated data preprocessing steps, including the extraction of NMR features corresponding to specific metabolites. We developed a novel 1D NMR feature e

APA, Harvard, Vancouver, ISO, and other styles

9

Karomah, Alfi Hudatul, Mohamad Rafi, Dewi Anggraini Septaningsih, et al. "UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts." HAYATI Journal of Biosciences 30, no. 4 (2023): 770–78. http://dx.doi.org/10.4308/hjb.30.4.770-778.

Full text

Abstract:

Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 3

APA, Harvard, Vancouver, ISO, and other styles

10

Mohamad Rafi, Mohamad Rafi, Triyani Hasanah Triyani Hasanah, Alfi Hudatul Karomah Alfi Hudatul Karomah, et al. "FTIR- and UHPLC-Q-Orbitrap HRMS-Based Metabolomics of Sonchus arvensis Extracts and Evaluation of Their Free Radical Scavenging Activity." Sains Malaysiana 51, no. 10 (2022): 3261–69. http://dx.doi.org/10.17576/jsm-2022-5110-12.

Full text

Abstract:

Sonchus arvensis, known as sowthistle, belongs to Asteraceae and contains numerous phenolic acids and flavonoids that exhibit antioxidants. The type of extraction solvent will affect biological activity level, resulting in different metabolite profiles that metabolomics approaches can evaluate by fingerprint analysis using FTIR spectrophotometry and UHPLC-Q-Orbitrap HRMS. Furthermore, the FTIR spectra and the metabolite profile of UHPLC-Q-Orbitrap HRMS were grouped using principal component analysis (PCA). This study aimed to clustering S. arvensis extracts based on different extracting solven

APA, Harvard, Vancouver, ISO, and other styles

11

Yang, Yang, Yanqiang Yin, Xianglan Tang, et al. "Evaluating Different Extraction Approaches for GC-MS Based Metabolomics Analysis of the Giant Pandas’ Fur." Toxics 10, no. 11 (2022): 688. http://dx.doi.org/10.3390/toxics10110688.

Full text

Abstract:

Giant pandas in zoo captivity are situated in residential areas, where environmental pollutants and anthropogenic factors have an impact on their health. Hair metabolomics has been applied in numerous environmental toxicological studies. Therefore, the panda fur metabolome could be a reliable approach to reflect endogenous and exogenous metabolic changes related to environmental exposure. However, there is no established extraction protocol to study the fur metabolome of pandas. The aim of this research was to optimize the extraction of panda fur metabolome for high-throughput metabolomics ana

APA, Harvard, Vancouver, ISO, and other styles

12

Kim, Hyeong Jun, Min Sun Choi, Shaheed Ur Rehman, et al. "Determination of Urinary Caffeine Metabolites as Biomarkers for Drug Metabolic Enzyme Activities." Nutrients 11, no. 8 (2019): 1947. http://dx.doi.org/10.3390/nu11081947.

Full text

Abstract:

Caffeine is commonly taken via the daily dietary consumption of caffeine-containing foods. The absorbed caffeine is metabolized to yield various metabolites by drug-metabolizing enzymes, and measuring the levels of each caffeine metabolite can provide useful information for evaluating the phenotypes of those enzymes. In this study, the urinary concentrations of caffeine and its 13 metabolites were determined, and the phenotypes of drug metabolic enzymes were investigated based on the caffeine metabolite ratios. Human urine samples were pretreated using solid phase extraction, and caffeine and

APA, Harvard, Vancouver, ISO, and other styles

13

Higgs, Richard E., James A. Zahn, Jeffrey D. Gygi, and Matthew D. Hilton. "Rapid Method To Estimate the Presence of Secondary Metabolites in Microbial Extracts." Applied and Environmental Microbiology 67, no. 1 (2001): 371–76. http://dx.doi.org/10.1128/aem.67.1.371-376.2001.

Full text

Abstract:

ABSTRACT Screening microbial secondary metabolites is an established method to identify novel biologically active molecules. Preparation of biological screening samples from microbial fermentation extracts requires growth conditions that promote synthesis of secondary metabolites and extraction procedures that capture the secondary metabolites produced. High-performance liquid chromatography (HPLC) analysis of fermentation extracts can be used to estimate the number of secondary metabolites produced by microorganisms under various growth conditions but is slow. In this study we report on a rap

APA, Harvard, Vancouver, ISO, and other styles

14

Bae, Hansol, Magnus Paludan, Jan Knoblauch, and Kaare H. Jensen. "Neural networks and robotic microneedles enable autonomous extraction of plant metabolites." Plant Physiology 186, no. 3 (2021): 1435–41. http://dx.doi.org/10.1093/plphys/kiab178.

Full text

Abstract:

Abstract Plant metabolites comprise a wide range of extremely important chemicals. In many cases, like savory spices, they combine distinctive functional properties—deterrence against herbivory—with an unmistakable flavor. Others have remarkable therapeutic qualities, for instance, the malaria drug artemisinin, or mechanical properties, for example natural rubber. We present a breakthrough in plant metabolite extraction technology. Using a neural network, we teach a computer how to recognize metabolite-rich cells of the herbal plant rosemary (Rosmarinus officinalis) and automatically extract t

APA, Harvard, Vancouver, ISO, and other styles

15

Sianturi, Parulian, Nur Asyiah Dalimunthe, Angga Ade Sahfitra, Rizal Aziz, and Saipul Sihotang. "IDENTIFIKASI KANDUNGAN SENYAWA METABOLIT SEKUNDER PADA BATANG DAN DAUN SURAT DIBATA (Macodes petola) MENGGUNAKAN GAS CHROMATOGRAPHY SPECTROMETRY (GCMS)." Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian 8, no. 2 (2024): 38–44. https://doi.org/10.31289/agr.v8i2.12725.

Full text

Abstract:

This study, entitled Identification of Secondary Metabolite Compounds in the Stems and Leaves of Surat Dibata (Macodes petola) Using Gas Chromatography Mass Spectrometry (GCMS) aims to determine the compounds and their concentrations of secondary metabolites present in the stems and leaves of Surat Dibata (Macodes petola) using Gas Chromatography Mass Spectrometry (GCMS). The methods used in this study include maceration extraction to identify secondary metabolite compounds and Gas Chromatography Mass Spectrometry (GCMS) to determine the concentrations of these compounds in the stems and leave

APA, Harvard, Vancouver, ISO, and other styles

16

Capece, Bettencourt P. S., Belén Pérez, Eugeni Castells, Margarita Arboix, and Carles Cristòfol. "Liquid Chromatographic Determination of Fenbendazole Residues in Pig Tissues after Treatment with Medicated Feed." Journal of AOAC INTERNATIONAL 82, no. 5 (1999): 1007–16. http://dx.doi.org/10.1093/jaoac/82.5.1007.

Full text

Abstract:

Abstract Fenbendazole (FBZ) is an anthelmintic widely used in farm animals to treat parasitic infestations, in pigs, it is administered in the food. The aim of this study was to validate an analytical method for the determination of FBZ and its metabolites in pig tissues. This method is based on oxidation of FBZ and its sulfoxide metabolite to the sulfone metabolite (FBZSO2). The limit of quantitation for this method is 20 ng FBZSO2/g for all tissues. The maximum residue limits (MRLs) established for FBZ and its metabolites in pig tissues are 50 ng/g for muscle, fat, and kidney and 500 ng/g fo

APA, Harvard, Vancouver, ISO, and other styles

17

Maya Dian Rakhmawatie, Maya Dian Rakhmawatie, Mustofa Mustofa, Puspita Lisdiyanti Puspita Lisdiyanti, Woro Rukmi Pratiwi Woro Rukmi Pratiwi, and Tri Wibawa Tri Wibawa. "Identification of Antimycobacterial from Actinobacteria (INACC A758) Secondary Metabolites using Metabolomics Data." Sains Malaysiana 51, no. 5 (2022): 1465–73. http://dx.doi.org/10.17576/jsm-2022-5105-16.

Full text

Abstract:

Actinobacteria produce active secondary metabolite with medicinal properties, such as antibacterial or anticancer. However, there are some reports about the difficulties in discovering novel secondary metabolites. Therefore, the need for a new approach is obvious. Several factors such as types of nutrients in the culture media or different solvents used for extraction have been proven to influence the Actinobacteria secondary metabolite production. In this study, a combination of culture media optimization and metabolites fingerprint analysis were applied to identify antimycobacterial active c

APA, Harvard, Vancouver, ISO, and other styles

18

Gerhard, Ute, Steven Thomas, and Russell Mortishire-Smith. "Accelerated metabolite identification by “Extraction-NMR”." Journal of Pharmaceutical and Biomedical Analysis 32, no. 3 (2003): 531–38. http://dx.doi.org/10.1016/s0731-7085(03)00218-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Floter, A., J. Nicolas, T. Schaub, and J. Selbig. "Threshold extraction in metabolite concentration data." Bioinformatics 20, no. 10 (2004): 1491–94. http://dx.doi.org/10.1093/bioinformatics/bth107.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Zhou, Mjn, Gui-Yun Li, and Stephanie A. Whalen. "Determination of Metsulfuron Methyl and Its Two Metabolites in Crops by Liquid Chromatography with Ultraviolet Detection." Journal of AOAC INTERNATIONAL 77, no. 6 (1994): 1654–59. http://dx.doi.org/10.1093/jaoac/77.6.1654.

Full text

Abstract:

Abstract Metsulfuron methyl, its hydroxy metabolite (A1), and its glucose conjugate metabolite (A) were determined in several crops by liquid chromatography (LC) using a simple extraction and cleanup scheme. After the parent and 2 major metabolites were extracted from crops with methanol, metabolite A was enzymatically hydrolyzed to metabolite A1 with β-glucosidase. The treated extracts were cleaned up chromatographically, and then metsulfuron methyl and metabolite A1 were quantitatively determined by reversed-phase LC with UV detection at 254 nm. Recovery of metsulfuron methyl and its 2 metab

APA, Harvard, Vancouver, ISO, and other styles

21

Valu, Mihai-Vlad, Liliana Cristina Soare, Nicoleta Anca Sutan, et al. "Optimization of Ultrasonic Extraction to Obtain Erinacine A and Polyphenols with Antioxidant Activity from the Fungal Biomass of Hericium erinaceus." Foods 9, no. 12 (2020): 1889. http://dx.doi.org/10.3390/foods9121889.

Full text

Abstract:

Hericium erinaceus is a medicinal fungal species that produces the active biological metabolite erinacine A with strong antioxidant activity. The classical extraction techniques used to date to obtain metabolites from this fungal species require high consumption of resources and energy and, in the end, prove to be expensive and inefficient, especially on a biomedical scale. The aim of this research is based on the development of an ultrasonic extraction (UE) method for the identification and extraction of biological compounds with high antioxidant activity from the mycelia of H. erinaceus biom

APA, Harvard, Vancouver, ISO, and other styles

22

Sellick, Christopher A., Rasmus Hansen, Gill M. Stephens, Royston Goodacre, and Alan J. Dickson. "Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling." Nature Protocols 6, no. 8 (2011): 1241–49. http://dx.doi.org/10.1038/nprot.2011.366.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Nickmilder, Marc J. M., Dominique Latinne, Jean-Paul De Houx, Roger K. Verbeeck, and Georges J. J. Lhoëst. "Isolation and identification of a C39 demethylated metabolite of rapamycin from pig liver microsomes and evaluation of its immunosuppressive activity." Clinical Chemistry 44, no. 3 (1998): 532–38. http://dx.doi.org/10.1093/clinchem/44.3.532.

Full text

Abstract:

Abstract We studied in vitro metabolism of rapamycin using pig liver microsomes. After extraction of the metabolites from the incubation medium, the crude metabolite extract was submitted to normal and subsequently to reversed-phase HPLC chromatography. We describe in the current study a metabolite of retention time 23.2 min collected from reversed-phase HPLC and identified by fast atom bombardment mass spectrometry (MS) and electrospray MS-MS as a C39 demethylated rapamycin metabolite. In vitro immunosuppressive activity of this metabolite, determined by the mixed lymphocyte reaction, was neg

APA, Harvard, Vancouver, ISO, and other styles

24

Kaleta, Alyona, Nadezhda Frolova, Anastasia Orlova, et al. "The Effects of Selected Extraction Methods and Natural Deep Eutectic Solvents on the Recovery of Active Principles from Aralia elata var. mandshurica (Rupr. & Maxim.) J. Wen: A Non-Targeted Metabolomics Approach." Pharmaceuticals 17, no. 3 (2024): 355. http://dx.doi.org/10.3390/ph17030355.

Full text

Abstract:

The methods and solvents employed in routine extraction protocols essentially impact the composition of the resulting extracts, i.e., the relative abundances of individual biologically active metabolites and the quality and stability of the isolates. Natural deep eutectic solvents (NADESs) represent a new class of environmentally friendly solvents, which are recognized as promising extractants alternative to conventional organic liquids. However, their relative efficiencies when applied in different extraction workflows are still poorly characterized. Therefore, here, we compare the potential

APA, Harvard, Vancouver, ISO, and other styles

25

Lia Fikayuniar, Adinda Khairun Nissa, Adiva Nafila Zulfa, et al. "Comparison of Metabolite Content between Water Extract and Ethanol Extract of Moringa Leaves (Moringa oleifera): A Systematic Literature Review." Eureka Herba Indonesia 4, no. 2 (2023): 237–41. http://dx.doi.org/10.37275/ehi.v4i2.75.

Full text

Abstract:

Extraction with water and ethanol are two common methods used to isolate secondary metabolites from Moringa oleifera. Extraction with water usually produces extracts rich in polar compounds, while extraction with ethanol tends to be better at isolating non-polar compounds. This study aimed to carry out a systematic review related to the comparative study of metabolite content between aqueous extracts and ethanol extracts of Moringa oleifera. The literature search process was carried out on various databases (PubMed, Web of Sciences, EMBASE, Cochrane Libraries, and Google Scholar) regarding the

APA, Harvard, Vancouver, ISO, and other styles

26

Singh, Vivek Vikram, Aisha Naseer, Gothandapani Sellamuthu, and Rastislav Jakuš. "An Optimized and Cost-Effective RNA Extraction Method for Secondary Metabolite-Enriched Tissues of Norway Spruce (Picea abies)." Plants 13, no. 3 (2024): 389. http://dx.doi.org/10.3390/plants13030389.

Full text

Abstract:

Since the development of next-generation sequencing techniques and with the growing interest in transcriptomic studies, there is a demand for high-throughput RNA extraction techniques. General RNA extraction protocols are unreliable when it comes to the quality and quantity of isolated RNA obtained from different tissue types of different plant species. Despite Norway spruce (Picea abies) being one of the most significant and commercially valuable tree species in European forests, only limited genetic research is available. In this study, we developed a cetyltrimethylammonium bromide (CTAB) pr

APA, Harvard, Vancouver, ISO, and other styles

27

Dasí-Navarro, Nuria, Sonia Lombardi, Pilar Vila-Donat, et al. "Metabolomic Profiling of Human Urine Related to Mycotoxin Exposure." Toxins 17, no. 2 (2025): 75. https://doi.org/10.3390/toxins17020075.

Full text

Abstract:

Human exposure to mycotoxins is a global concern since several mycotoxins, such as enniatins and aflatoxins, have shown carcinogenic and neurotoxic effects, and the toxicologic mechanisms of most of them still need to be clarified. This study aims to investigate the metabolic pathways affected by mycotoxin exposure by evaluating metabolite alterations in urine. The participants were 540 women from the Spanish Childhood and Environment Project (INMA). For metabolite identification, a dilute and shoot extraction, followed by HPLC-Q-TOF-MS identification analysis, was performed. Data were process

APA, Harvard, Vancouver, ISO, and other styles

28

Nováková, Slavomíra, Eva Baranovičová, Zuzana Hatoková, et al. "Comparison of Various Extraction Approaches for Optimized Preparation of Intracellular Metabolites from Human Mesenchymal Stem Cells and Fibroblasts for NMR-Based Study." Metabolites 14, no. 5 (2024): 268. http://dx.doi.org/10.3390/metabo14050268.

Full text

Abstract:

Metabolomics has proven to be a sensitive tool for monitoring biochemical processes in cell culture. It enables multi-analysis, clarifying the correlation between numerous metabolic pathways. Together with other analysis, it thus provides a global view of a cell’s physiological state. A comprehensive analysis of molecular changes is also required in the case of mesenchymal stem cells (MSCs), which currently represent an essential portion of cells used in regenerative medicine. Reproducibility and correct measurement are closely connected to careful metabolite extraction, and sample preparation

APA, Harvard, Vancouver, ISO, and other styles

29

Agustina, S., S. Bella, S. Karina, I. Irwan, and M. Ulfah. "Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique." IOP Conference Series: Earth and Environmental Science 869, no. 1 (2021): 012010. http://dx.doi.org/10.1088/1755-1315/869/1/012010.

Full text

Abstract:

Abstract Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary met

APA, Harvard, Vancouver, ISO, and other styles

30

Salazar-González, Claudia, Carolina Mendoza Ramos, Hugo A. Martínez-Correa, and Hugo Fabián Lobatón García. "Extraction and Concentration of Spirulina Water-Soluble Metabolites by Ultrafiltration." Plants 13, no. 19 (2024): 2770. http://dx.doi.org/10.3390/plants13192770.

Full text

Abstract:

Spirulina (Arthospira platensis) is known for its rich content of natural compounds like phycocyanin, chlorophylls, carotenoids, and high protein levels, making it a nutrient-dense food. Over the past decade, research has aimed to optimize the extraction, separation, and purification of these valuable metabolites, focusing on technologies such as high-pressure processing, ultrasound-assisted extraction, and microwave-assisted extraction as well as enzymatic treatments, chromatographic precipitation, and membrane separation. In this study, various extraction methods (conventional vs. ultrasound

APA, Harvard, Vancouver, ISO, and other styles

31

Srivastava, Nidhi, Vishal Dubey, Madhumita Sengar, and Rastogi Sameer. "Identification and Characterization of Metabolites of Irinotecan in-vivo and in-vitro matrixes by HPLC/LC-MS." Research in Pharmacy and Health Sciences 2, no. 3 (2016): 211–18. http://dx.doi.org/10.32463/rphs.2016.v02i03.42.

Full text

Abstract:

In the present study metabolite identification and characterization has done by using HPLC and LC-MS. During method development various mobile phases have tried for identification of metabolites. The matrixes selected for in- vivo study were urine because nearly all the metabolites of irinotecan were obtained in it. The extraction mixtures have selected to retain maximum amount of analyte with less effort. During experiment four extraction solvents were used in six different concentrations out of which TBME suit our method. In-vitro study done by Human Liver microsomes by using Phosphate buffe

APA, Harvard, Vancouver, ISO, and other styles

32

Medina, Jessica, Vera van der Velpen, Tony Teav, Yann Guitton, Hector Gallart-Ayala, and Julijana Ivanisevic. "Single-Step Extraction Coupled with Targeted HILIC-MS/MS Approach for Comprehensive Analysis of Human Plasma Lipidome and Polar Metabolome." Metabolites 10, no. 12 (2020): 495. http://dx.doi.org/10.3390/metabo10120495.

Full text

Abstract:

Expanding metabolome coverage to include complex lipids and polar metabolites is essential in the generation of well-founded hypotheses in biological assays. Traditionally, lipid extraction is performed by liquid-liquid extraction using either methyl-tert-butyl ether (MTBE) or chloroform, and polar metabolite extraction using methanol. Here, we evaluated the performance of single-step sample preparation methods for simultaneous extraction of the complex lipidome and polar metabolome from human plasma. The method performance was evaluated using high-coverage Hydrophilic Interaction Liquid Chrom

APA, Harvard, Vancouver, ISO, and other styles

33

Andiarna, Funsu, Mei Lina Fitri Kumalasari, Esti Tyastirin, Eko Teguh Pribadi, Romyun Alvy Khoiriyah, and Sarita Oktorina. "IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA EKSTRAK METHANOL BATANG KEMANGI (Ocimum bacilicum L)." GEMA KESEHATAN 15, no. 2 (2023): 103–9. http://dx.doi.org/10.47539/gk.v15i2.420.

Full text

Abstract:

Tanaman kemangi (Ocimum bacilicum L) adalah salah satu tanaman obat tradisional yang paling banyak dikembangkan di negara Indonesia. Tanaman kemangi memiliki banyak manfaat seperti mengobati demam, diare, batuk, pusing, mual, dan lain sebagainya. Tujuan dari penelitian ini untuk mengidentifikasi senyawa metabolit sekunder pada batang tanaman kemangi. Ekstrak kemangi menggunakan pelarut metanol 96%. Batang daun kemangi dilakukan proses penyarian dengan metode maserasi. Ekstrak kemangi selanjutnya dilakukan uji fitokimia untuk mengetahui kandungan senyawa metabolit sekunder. Hasil dari penelitia

APA, Harvard, Vancouver, ISO, and other styles

34

Mutiah, Roihatul, Weka Sidha Bhagawan, Burhan Ma'arif, and Jauhar Maknun Septaza Rahmandika. "Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS." Majalah Obat Tradisional 24, no. 3 (2019): 139. http://dx.doi.org/10.22146/mot.44883.

Full text

Abstract:

Eleutherine palmifolia (L.) Merr. (E. palmifolia) is an Indonesian native plant that has the potential to be developed into phytopharmaca. The differences in growth locations are thought to cause variation in the content of metabolite compounds which affect differences in pharmacological activity. This study aims to determine the profile of metabolites E. palmifolia bulb from several regions in Indonesia. The samples were collected from six different locations, namely East Java, Central Java, West Java, East Borneo, Central Borneo, and South Borneo. Sample extraction was carried out using Ultr

APA, Harvard, Vancouver, ISO, and other styles

35

K., Sathiyamurthy, and Bavithra H. "Bioactive potential of Pseudomonas alcaliphila isolated from a marine sponge against human pathogens." International Journal of Bio-Pharma Research 8, no. 3 (2019): 2494–22503. http://dx.doi.org/10.21746/ijbpr.2019.8.3.2.

Full text

Abstract:

Metabolite extraction is considered as one of the important steps in metabolomics, the marine metabolite are the new source of the most antimicrobial agents used in both pharmacological and biological applications. In the present study, sponge associated bacterial metabolites was investigated. A total of 20 bacterial strains were isolated from the sponge Haliclona sp., All the strains were screened primarily with cross streaking method against human bacterial pathogens. The potent isolate was chosen based on the good inhibitory activity and metabolite extraction was achieved using chloroform:

APA, Harvard, Vancouver, ISO, and other styles

36

Lukito, Evul Winoto, Dyah Iswantini, Budhi Antariksa, Mohamad Rafi, and Setyanto Tri Wahyudi. "Metabolite Profiling and Inhibitory Effects of Nitric Oxide on Andrographis paniculata Burm. F Nees Extract using Different Solvents as a Potential Candidate in COVID-19 Therapy." Asian Journal of Chemistry 36, no. 6 (2024): 1301–7. http://dx.doi.org/10.14233/ajchem.2024.31387.

Full text

Abstract:

Andrographis paniculata Burm. F Nees used to treat COVID-19 in several Asian countries because it has metabolites with pharmacological activity that may decrease the pathogenesis features of COVID-19. The extraction solvent significantly affected the composition and concentration of the metabolites. Therefore, the inhibitory effects of nitric oxide of A. paniculata extract based on differences in extraction solvent concentrations were investigare and also identified its metabolite profile. The leaves of A. paniculata were extracted with three different solvents like water solvent, 50% ethanol

APA, Harvard, Vancouver, ISO, and other styles

37

Dias, Eduardo Antunes, Marcilio Nichi, and Marcelo A. B. V. Guimarães. "Comparison of two commercial kits and two extraction methods for fecal glucocorticoid analysis in ocelots (Leopardus pardalis) submitted to ACTH challenge." Pesquisa Veterinária Brasileira 28, no. 7 (2008): 329–34. http://dx.doi.org/10.1590/s0100-736x2008000700002.

Full text

Abstract:

The ocelot (Leopardus pardalis) is included in list of wild felid species protected by CITES and is part of conservation strategies that necessarily involve the use of assisted reproduction techniques, which requires practical and minimally invasive techniques of high reproducibility that permit the study of animal reproductive physiology. The objective of this study was to compare and validate two commercial assays: ImmuChem Double Antibody Corticosterone 125I RIA from ICN Biomedicals, Costa Mesa, CA, USA; and Coat-a-Count Cortisol 125I RIA from DPC, Los Angeles, CA, USA, for assessment of fe

APA, Harvard, Vancouver, ISO, and other styles

38

Li, Jing, Ju Liu, Jennifer Enders, et al. "Discovery of a novel deaminated metabolite of a single-stranded oligonucleotide in vivo by mass spectrometry." Bioanalysis 11, no. 21 (2019): 1955–65. http://dx.doi.org/10.4155/bio-2019-0118.

Full text

Abstract:

Aim: A novel single-stranded deaminated oligonucleotide metabolite resulting from a REVERSIR™ oligonucleotide was discovered and identified in monkey liver after subcutaneous administration. Results & methodology: REVERSIR-A and its metabolites were extracted from biological matrices by solid phase extraction and analyzed using LC coupled with high-resolution MS under negative ionization mode. A novel 9-mer metabolite of REVERSIR-A, resulting from deamination of the 3′ terminal 2′- O-methyl-adenosine nucleotide to 2′- O-methyl-inosine, was discovered at significant levels in monkey liver.

APA, Harvard, Vancouver, ISO, and other styles

39

Erum, Saira, Sadia Sultan, Syed Adnan Ali Shah, Muhammad Ashraf, and Muhammad Iqbal Choudhary. "MICROBIAL OXIDATION OF FINASTERIDE WITH MACROPHOMINA PHASEOLINA(KUCC 730)." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 10 (2017): 17. http://dx.doi.org/10.22159/ijpps.2017v9i11.13576.

Full text

Abstract:

Objectives: New microbial oxidative derivatives of Finasteride [17β-(N-tert-butylcarbamoyl)-4-aza-5α-androst-1-en-3-one] (1) has been investigated with Macrophomina phaseolina (ATCC730).Methods: Fermented media of Macrophomina phaseolina (ATCC730) was prepared to cultivate the fungal cultures . Substrate 1 was incubated in liquid media for 16 days. After sixteen days, filtration and extraction of the fermented media was carried out with 9 L DCM in three portions. Resulting organic extract was dried using anhydrous (Na2SO4), and evaporated to afford a brown gum (950 mg). This on chromatographic

APA, Harvard, Vancouver, ISO, and other styles

40

Maxim, Claudia, Alexandra Cristina Blaga, Ramona-Elena Tataru-Farmus, and Daniela Suteu. "Acmella oleracea Metabolite Extraction Using Natural Deep Eutectic Solvents." Processes 12, no. 8 (2024): 1686. http://dx.doi.org/10.3390/pr12081686.

Full text

Abstract:

For plant metabolite extraction, natural deep eutectic solvents (NADESs) have many benefits over conventional solvents and ionic liquids. These advantages include high solubility and extraction ability, a low melting point (<100 °C), low toxicity, environmental friendliness, recyclability, and better biodegradability. This study analyses a natural deep eutectic solvent for Acmella oleracea (A. oleracea) metabolite extraction, considering the following process parameters: temperature, component ratio in the eutectic solvent, water addition, solid/liquid ratio, and extraction duration. NADESs

APA, Harvard, Vancouver, ISO, and other styles

41

Zhao, Jing, Ju Tang, Zhandi Wang, et al. "Extraction, analysis, and antifungal activity study of algae antibiotic active substances in plateau lakes." PLOS One 20, no. 5 (2025): e0319853. https://doi.org/10.1371/journal.pone.0319853.

Full text

Abstract:

This study was carried out to assess the inhibitory activity of algae in plateau lakes against plant pathogenic fungi, and further conduct preliminary research and analysis on their antifungal active ingredients, in order to provide a certain basis for the development and utilization of algal secondary metabolite as anti-plant pathogenic fungal agents. Different solvent extraction methods using water, ethyl acetate, ethanol, and methanol were conducted to extract polyphenol metabolites from Ulothrix, Chlorella vulgaris, and Microcystis pseudofilamentosa Crow. The composition of the extracts wa

APA, Harvard, Vancouver, ISO, and other styles

42

Rashid, Faraz, Sudeep Ghimire, Ashutosh K. Mangalam, and Shailendra Giri. "A UPLC-MS/MS Based Rapid, Sensitive, and Non-Enzymatic Methodology for Quantitation of Dietary Isoflavones in Biological Fluids." Molecules 28, no. 18 (2023): 6729. http://dx.doi.org/10.3390/molecules28186729.

Full text

Abstract:

Dietary isoflavones, a type of phytoestrogens, have gained importance owing to their health-promoting benefits. However, the beneficial effects of isoflavones are mediated by smaller metabolites produced with the help of gut bacteria that are known to metabolize these phytoestrogenic compounds into Daidzein and Genistein and biologically active molecules such as S-Equol. Identifying and measuring these phytoestrogens and their metabolites is an important step towards understanding the significance of diet and gut microbiota in human health and diseases. We have overcome the reported difficulti

APA, Harvard, Vancouver, ISO, and other styles

43

Wahid, Abdul Rahman, and Safwan Safwan. "Skrining Fitokimia Senyawa Metabolit Sekunder Terhadap Ekstrak Tanaman Ranting Patah Tulang (Euphorbia tirucalli L.)." Lumbung Farmasi: Jurnal Ilmu Kefarmasian 1, no. 1 (2020): 24. http://dx.doi.org/10.31764/lf.v1i1.1208.

Full text

Abstract:

ABSTRAKTanaman patah tulang termasuk dalam famili Euphorbiaceae, merupakan jenis tanaman kebun yang tumbuh tegak hingga setinggi 2-6m. Kandungan senyawa metabolit sekunder tanaman Euphorbia tirucalli L yaitu flavonid, fenol, saponin, dan tanin Penelitian ini dilakukan untuk mengetahui kandungan senyawa metabolit sekunder dan aktivitas antioksidan daun ranting tanaman patah tulang. Senyawa metabolit sekunder diperoleh dari proses ekstraksi dengan pelarut etanol menggunakan metode maserasi dan partisi. Metode penelitian skrining fitokimia dilakukan dengan uji warna menggunakan berbagai pereaksi.

APA, Harvard, Vancouver, ISO, and other styles

44

Rathore, Madhurjit Singh, Sushma Tiwari, Neeta Dwivedi, and Nimmy MS. "Optimized DNA Isolation Protocol from Pearl Millet (Pennisetum glaucum) Leaves with High Phenolic and Metabolite Content." PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY 26, no. 5-6 (2025): 67–75. https://doi.org/10.56557/pcbmb/2025/v26i5-69292.

Full text

Abstract:

Pearl millet (Pennisetum glaucum [L.] R. Br.) is a vital cereal crop recognized for its resilience to harsh environmental conditions. However, extracting high-quality DNA from pearl millet leaves presents a significant challenge due to the presence of phenolic compounds and secondary metabolites that interfere with molecular biology procedures. This study optimized a DNA isolation protocol tailored for pearl millet genotypes with high phenol and metabolite content to ensure the extraction of pure and intact DNA suitable for downstream molecular applications. Fresh leaf tissues from ten pearl m

APA, Harvard, Vancouver, ISO, and other styles

45

Ma'arif, Burhan, Agnis Aditama, Roihatul Muti'ah, Weka Sidha Bagawan, Reyhan Amiruddin, and Rukiana Rukiana. "PROFIL METABOLIT BERBAGAI EKSTRAK DAUN Chrysophyllum cainito L. MENGGUNAKAN UPLC-QTOF-MS/MS." Jurnal Tumbuhan Obat Indonesia 12, no. 1 (2019): 10–24. http://dx.doi.org/10.22435/jtoi.v12i1.672.

Full text

Abstract:

ABSTRACT Chrysophyllum cainito L. is a plant which empirically used as traditional medicine. The pharmacological effect of C. cainito is caused by secondary metabolite activity contain in the leaves. The aim of this research was to know the metabolites profile in n-hexane extract, ethyl acetate extract, and methanol extract of C. cainito leaves using UPLC-QToF-MS/MS. Dried powder of C. cainito leaves was extracted with n-hexane, ethyl acetate, and methanol with gradual extraction using Ultrasonic Assisted Extraction (UAE). Each extract was prepared with methanol and DCM solvent then inje

APA, Harvard, Vancouver, ISO, and other styles

46

Shen, Huihui, Xiuxian Song, Yue Zhang, et al. "Profiling of Brevetoxin Metabolites Produced by Karenia brevis 165 Based on Liquid Chromatography-Mass Spectrometry." Toxins 13, no. 5 (2021): 354. http://dx.doi.org/10.3390/toxins13050354.

Full text

Abstract:

In this study, Karenia brevis 165 (K. brevis 165), a Chinese strain, was used to research brevetoxin (BTX) metabolites. The sample pretreatment method for the enrichment of BTX metabolites in an algal culture medium was improved here. The method for screening and identifying intracellular and extracellular BTX metabolites was established based on liquid chromatography-time-of-flight mass spectrometry (LC-ToF-MS) and liquid chromatography triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS). The results show that the recovery rates for BTX toxins enriched by a hydrophilic–lipophilic balanc

APA, Harvard, Vancouver, ISO, and other styles

47

El-Shafei, Rasha, Hala Hegazy, and Bishnu Acharya. "A Review of Antiviral and Antioxidant Activity of Bioactive Metabolite of Macroalgae within an Optimized Extraction Method." Energies 14, no. 11 (2021): 3092. http://dx.doi.org/10.3390/en14113092.

Full text

Abstract:

Non-conventional extraction of bioactive metabolites could provide sustainable alternative techniques to preserve the potency of antioxidants and antiviral compounds extracted from macro-algae. In this paper, we first reviewed the antioxidant and antiviral potential of the active metabolites that exist in the three known macro-algae classes; Phaeophyceae, Rhodophyceae, and Chlorophyceae, and a comparison between their activities is discussed. Secondly, a review of conventional and non-conventional extraction methods is undertaken. The review then focused on identifying the optimal extraction m

APA, Harvard, Vancouver, ISO, and other styles

48

Adfa, Morina, Dina Erliana, Khafit Wiradimafan, et al. "Application of FTIR Spectroscopy and Chemometric to Differentiate Azadirachta excelsa (Jack.) Jacobs Leaves Extracts Based on Solvent Polarity and Assessment of Antibacterial Activity." Molekul 19, no. 2 (2024): 309. http://dx.doi.org/10.20884/1.jm.2024.19.2.10790.

Full text

Abstract:

ABSTRACT. Azadirachta excelsa is a plant belonging to the same genus as the Indian neem (Azadirachta indica) which is expected to have similar biological activities. However, its active components and pharmacological effects are limited. The composition and quantity of these metabolites in A. excelsa may differ due to different polarities of extracting solvents, so selecting an effective extractive solvent with a high level of biological activity is important. Therefore, in this study, we examined differences in the metabolite finger printing using FTIR-based metabolomics, as well as evaluated

APA, Harvard, Vancouver, ISO, and other styles

49

Cedillo-Portillo, José Juan, Wendy Yaneth Villastrigo-López, Adali Oliva Castañeda-Facio, Sandra Cecilia Esparza-González, Elia Martha Múzquiz-Ramos, and Aidé Sáenz-Galindo. "Salvia rosmarinus Spenn. Main Applications and Ultrasonic Extraction of Secondary Metabolites: a General Review." Revista Mexicana de Ingeniería Biomédica 45, no. 2 (2024): 35–61. http://dx.doi.org/10.17488/rmib.45.2.3.

Full text

Abstract:

This paper provides an overview of the various applications of the bioactive compounds found in S. rosmarinus. at present. Additionally, it explores emerging technologies for its extraction, such as ultrasound, which is an effective, fast, and sustainable technology for obtaining these secondary metabolites from this millenary plant. S. rosmarinus has gained considerable importance due to its beneficial properties, including antimicrobial, antioxidant, hepatoprotective, anti-inflammatory, and anticarcinogenic effects. These effects result from the different metabolites, which, without the use

APA, Harvard, Vancouver, ISO, and other styles

50

Kelly, Patricia E., H. Jene Ng, Gillian Farrell, et al. "An Optimised Monophasic Faecal Extraction Method for LC-MS Analysis and Its Application in Gastrointestinal Disease." Metabolites 12, no. 11 (2022): 1110. http://dx.doi.org/10.3390/metabo12111110.

Full text

Abstract:

Liquid chromatography coupled with mass spectrometry (LC-MS) metabolomic approaches are widely used to investigate underlying pathogenesis of gastrointestinal disease and mechanism of action of treatments. However, there is an unmet requirement to assess faecal metabolite extraction methods for large-scale metabolomics studies. Current methods often rely on biphasic extractions using harmful halogenated solvents, making automation and large-scale studies challenging. The present study reports an optimised monophasic faecal extraction protocol that is suitable for untargeted and targeted LC-MS

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!