Academic literature on the topic 'Metacyclic Trypomastigotes'

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Journal articles on the topic "Metacyclic Trypomastigotes"

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Yoshida, N., M. M. G. Teixeira, and C. A. Sbravate. "Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi." Revista do Instituto de Medicina Tropical de São Paulo 28, no. 2 (1986): 80–86. http://dx.doi.org/10.1590/s0036-46651986000200003.

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Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein
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Bahia, Maria Terezinha, Washington Luiz Tafuri, Marcelo Vidigal Caliari, et al. "Comparison of Trypanosoma cruzi infection in dogs inoculated with blood or metacyclic trypomastigotes of Berenice-62 and Berenice-78 strains via intraperitoneal and conjunctival routes." Revista da Sociedade Brasileira de Medicina Tropical 35, no. 4 (2002): 339–45. http://dx.doi.org/10.1590/s0037-86822002000400010.

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This paper aimed to verify the influence of the inoculum source (blood or metacyclic trypomastigote) and the route of inoculation (intraperitoneal or conjunctival) on the course of T. cruzi infection in dogs, using comparatively the T. cruzi strains Berenice-62 and Berenice-78. All dogs inoculated intraperitoneally became infected independently of the T. cruzi strain and source of trypomastigotes used. High level of infectivity was also observed when metacyclic trypomastigotes of both strains were inoculated by conjunctival route. However, when blood trypomastigotes were inoculated by conjunct
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VIDAL, JULIANA C., CAROLINA DE L. ALCANTARA, WANDERLEY DE SOUZA, and NARCISA L. CUNHA-E-SILVA. "Lysosome-like compartments of Trypanosoma cruzi trypomastigotes may originate directly from epimastigote reservosomes." Parasitology 144, no. 6 (2017): 841–50. http://dx.doi.org/10.1017/s0031182016002602.

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SUMMARYTrypanosoma cruzi epimastigote reservosomes store nutrients taken up during the intense endocytic activity exhibited by this developmental form. Reservosomes were classified as pre-lysosomal compartments. In contrast, trypomastigote forms are not able to take up nutrients from the medium. Interestingly, trypomastigotes also have acidic organelles with the same proteases contained in epimastigote reservosomes. Nevertheless, the origin and function of these organelles have not been disclosed so far. Given the similarities between the compartments of epimastigotes and trypomastigotes, the
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de Souza, Wanderley, Tecia Maria Ulisses de Carvalho, and Emile Santos Barrias. "Review onTrypanosoma cruzi: Host Cell Interaction." International Journal of Cell Biology 2010 (2010): 1–18. http://dx.doi.org/10.1155/2010/295394.

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Trypanosoma cruzi, the causative agent of Chagas' disease, which affects a large number of individuals in Central and South America, is transmitted to vertebrate hosts by blood-sucking insects. This protozoan is an obligate intracellular parasite. The infective forms of the parasite are metacyclic and bloodstream trypomastigote and amastigote. Metacyclic trypomastigotes are released with the feces of the insect while amastigotes and bloodstream trypomastigotes are released from the infected host cells of the vertebrate host after a complex intracellular life cycle. The recognition between para
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Contreras, Victor T., Tania C. de Araújo-Jorge, Myrna C. Bonaldo, et al. "Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media." Memórias do Instituto Oswaldo Cruz 83, no. 1 (1988): 123–33. http://dx.doi.org/10.1590/s0074-02761988000100016.

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The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to
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Zeledon, Rodrigo, Rodolfo Bolaños, M. R. Espejo Navarro, and Miguel Rojas. "Morphological evidence by scanning electron microscopy of excretion of metacyclic forms of Trypanosoma cruzi in vector's urine." Memórias do Instituto Oswaldo Cruz 83, no. 3 (1988): 361–65. http://dx.doi.org/10.1590/s0074-02761988000300014.

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Comparision by scanning electron microscopy (SEM) of Trypanosoma cruzi flagellates attached to the cuticle of the rectal gland of infected Dipetalogaster maxima nymphs, showed marked differences before amd after feeding. Before feeding numerous metacyclic trypomastigotes were observed among the abundant epimastigotes that formed the carpet of flagellates. On the other hand, in insects that were allowed to urinate for 24 hours after a meal, the metacyclics were scarce,indicating that they had been detached by the urine flow. An asymetric type of cell division, probably originating both an epi-a
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Neira, Ivan, Fernando A. Silva, Mauro Cortez, and Nobuko Yoshida. "Involvement of Trypanosoma cruzi Metacyclic Trypomastigote Surface Molecule gp82 in Adhesion to Gastric Mucin and Invasion of Epithelial Cells." Infection and Immunity 71, no. 1 (2003): 557–61. http://dx.doi.org/10.1128/iai.71.1.557-561.2003.

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ABSTRACT Upon oral infection, Trypanosoma cruzi metacyclic trypomastigotes invade and replicate in the gastric mucosal epithelium, being apparently uniquely specialized for adhesion to mucin and mucosal invasion. Here we investigated the involvement of gp82, the metacyclic-stage-specific surface glycoprotein implicated in host cell entry, in both adhesion to gastric mucin and invasion of the mucosal epithelium upon oral challenge. Metacyclic forms, preincubated with a control monoclonal antibody (MAb) or with MAb 3F6 directed to gp82, were administered orally to BALB/c mice, and parasitemia wa
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Kulkarni, Manjusha M., Cheryl L. Olson, David M. Engman, and Bradford S. McGwire. "Trypanosoma cruzi GP63 Proteins Undergo Stage-Specific Differential Posttranslational Modification and Are Important for Host Cell Infection." Infection and Immunity 77, no. 5 (2009): 2193–200. http://dx.doi.org/10.1128/iai.01542-08.

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ABSTRACT The protozoan Trypanosoma cruzi expresses multiple isoforms of the GP63 family of metalloproteases. Polyclonal antiserum against recombinant GP63 of T. cruzi (TcGP63) was used to study TcGP63 expression and localization in this organism. Western blot analysis revealed that TcGP63 is 61 kDa in epimastigotes, amastigotes, and tissue culture-derived trypomastigotes but 55 kDa in metacyclic trypomastigotes. Antiserum specific for Leishmania amazonensis GP63 specifically reacted with a 55-kDa TcGP63 form in metacyclic trypomastigotes, suggesting stage-specific expression of different isofo
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Nardelli, Sheila Cristina, Andréa Rodrigues Ávila, Aline Freund, et al. "Small-Subunit rRNA Processome Proteins Are Translationally Regulated during Differentiation of Trypanosoma cruzi." Eukaryotic Cell 6, no. 2 (2006): 337–45. http://dx.doi.org/10.1128/ec.00279-06.

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ABSTRACT We used differential display to select genes differentially expressed during differentiation of epimastigotes into metacyclic trypomastigotes in the protozoan parasite Trypanosoma cruzi. One of the selected clones had a sequence similar to that of the small-subunit (SSU) processome protein Sof1p, which is involved in rRNA processing. The corresponding T. cruzi protein, TcSof1, displayed a nuclear localization and is downregulated during metacyclogenesis. Heterologous RNA interference assays showed that depletion of this protein impaired growth but did not affect progression through th
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Ferreira, Ludmila R. P., Fernando de M. Dossin, Thiago C. Ramos, Edna Freymüller, and Sergio Schenkman. "Active transcription and ultrastructural changes during Trypanosoma cruzi metacyclogenesis." Anais da Academia Brasileira de Ciências 80, no. 1 (2008): 157–66. http://dx.doi.org/10.1590/s0001-37652008000100011.

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The differentiation of proliferating epimastigote forms of Trypanosoma cruzi , the protozoan parasite that causes Chagas’ disease, into the infective and non-proliferating metacyclic forms can be reproduced in the laboratory by incubating the cells in a chemically-defined medium that mimics the urine of the insect vector. Epimastigotes have a spherical nucleus, a flagellum protruding from the middle of the protozoan cell, and a disk-shaped kinetoplast - an organelle that corresponds to the mitochondrial DNA. Metacyclic trypomastigotes have an elongated shape with the flagellum protruding from
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Dissertations / Theses on the topic "Metacyclic Trypomastigotes"

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Ramos, Bruno Dias. "Caracterização molecular de proteínas hipotéticas diferencialmente expressas em Tripamastigota Metacíclico." Instituto Carlos Chagas, 2014. https://www.arca.fiocruz.br/handle/icict/8948.

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Submitted by Andrea Hartke (andreamh@fiocruz.br) on 2014-11-24T13:25:54Z No. of bitstreams: 1 Dissertação Bruno Dias Ramos.pdf: 4916835 bytes, checksum: fa6000cbfecb89598eef24d308186fc7 (MD5)<br>Approved for entry into archive by Andrea Hartke (andreamh@fiocruz.br) on 2014-11-24T17:25:03Z (GMT) No. of bitstreams: 1 Dissertação Bruno Dias Ramos.pdf: 4916835 bytes, checksum: fa6000cbfecb89598eef24d308186fc7 (MD5)<br>Made available in DSpace on 2014-11-24T17:25:03Z (GMT). No. of bitstreams: 1 Dissertação Bruno Dias Ramos.pdf: 4916835 bytes, checksum: fa6000cbfecb89598eef24d308186fc7 (MD5) Previ
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