Academic literature on the topic 'Metaphase arrangement'

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Journal articles on the topic "Metaphase arrangement"

1

Beçak, Maria Luiza, Willy Beçak, and Alexandre Pereira. "Somatic pairing, endomitosis and chromosome aberrations in snakes (Viperidae and Colubridae)." Anais da Academia Brasileira de Ciências 75, no. 3 (2003): 285–300. http://dx.doi.org/10.1590/s0001-37652003000300004.

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The positioning of macrochromosomes of Bothrops jararaca and Bothrops insularis (Viperidae) was studied in undistorted radial metaphases of uncultured cells (spermatogonia and oogonia) not subjected to spindle inhibitors. Colchicinized metaphases from uncultured (spleen and intestine) and cultured tissues (blood) were also analyzed. We report two antagonic non-random chromosome arrangements in untreated premeiotic cells: the parallel configuration with homologue chromosomes associated side by side in the metaphase plate and the antiparallel configuration having homologue chromosomes with antipolar distribution in the metaphase ring. The antiparallel aspect also appeared in colchicinized cells. The spatial chromosome arrangement in both configurations is groupal size-dependent and maintained through meiosis. We also describe, in untreated gonia cells, endomitosis followed by reductional mitosis which restores the diploid number. In B. jararaca males we observed that some gonad regions present changes in the meiotic mechanism. In this case, endoreduplicated cells segregate the diplochromosomes to opposite poles forming directly endoreduplicated second metaphases of meiosis with the suppression of first meiosis. By a successive division, these cells form nuclei with one set of chromosomes. Chromosome doubling in oogonia is known in hybrid species and in parthenogenetic salamanders and lizards. This species also presented chromosome rearrangements leading to aneuploidies in mitosis and meiosis. It is suggested that somatic pairing, endomitosis, meiotic alterations, and chromosomal aberrations can be correlated processes. Similar aspects of nuclei configurations, endomitosis and reductional mitosis were found in other Viperidae and Colubridae species.
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2

Goicoechea, P. G., A. Roca, A. R. Linde, T. Naranjo, and R. Giraldez. "Independent arrangement of bivalents and (or) quadrivalents in linear meiotic metaphase plates of rye." Genome 34, no. 3 (1991): 421–29. http://dx.doi.org/10.1139/g91-064.

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The relative positioning of bivalents and (or) quadrivalents in flattened lateral views of metaphase I (linear metaphase plates) has been analyzed in three different plant types of rye: normal plants (type 1); heterozygotes for translocation T305W (type 2); and double heterozygotes for translocations T305W and TR01 (type 3). In all plant types all bivalents and (or) quadrivalents were identified using C-banding. The results indicate that quadrivalents show a preference towards being located in marginal positions of the linear plate, and there are also differences in position preferences between specific bivalents. Adjacently oriented quadrivalents and rod bivalents show a stronger preference for marginal positions than alternate quadrivalents and ring bivalents, respectively, but this does not indicate the existence of a fixed or ordered arrangement of chromosomes in the spindle since bivalents and (or) quadrivalents are independently located relative to each other.Key words: Secale, meiosis, metaphase, arrangement, multivalents, bivalents.
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3

Kaur, Harbhajan, and Vikas Suman. "Chromosomes and their meiotic behaviour in two species of Dieuches Dohrn, 1860 (Heteroptera: Lygaeidae: Rhyparochromini)." Comparative Cytogenetics 3, no. (1) (2009): 43–50. https://doi.org/10.3897/compcytogen.v3i1.7.

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The Lygaeidae (Heteroptera) are a large and diverse family in which the male diploid chromosomal complement ranges from 10 to 30. Diploid numbers of 14 and 16 are taken as two modal numbers of the family. The Rhyparochrominae, one of the largest subfamilies of the Lygaeidae, are known to be heterogeneous both cytologically and morphologically. Available data on the tribe Rhyparochromini reveal that all species are characterized by the presence of a pair of microchromosomes (m-chromosomes) and have an XY/XX (♂/♀) sex chromosome determining system.<em> Dieuches</em> <em>coloratus</em> (Distant, 1909) and <em>D. insignis </em>(Distant, 1918) belonging to Rhyparochromini, have 2n=14=10A+2m+XY and<em> </em>2n=12=8A+2m+XY respectively. Both the species are similar inone pair of distinctly large autosomes in their chromosome complements. The metaphase plate arrangement of autosomes, sex chromosomes and m-chromosomes in <em>D. coloratus</em> is similar to the common condition observed in the tribe Rhyparochromini. In <em>D. insignis,</em> however, the arrangement is different. Here<em>, </em>metaphase I<em> </em>is usual in showing peripheral position of autosomes and central position of sex chromosomes and m-chromosomes. At metaphase II, however, autosomes, sex chromosomes and m-chromosomes are peripherally placed, an arrangement, which is not reported earlier in the tribe Rhyparochromini.
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4

Schubert, Veit, Mateusz Zelkowski, Sonja Klemme, and Andreas Houben. "Similar Sister Chromatid Arrangement in Mono- and Holocentric Plant Chromosomes." Cytogenetic and Genome Research 149, no. 3 (2016): 218–25. http://dx.doi.org/10.1159/000447681.

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Due to the X-shape formation at somatic metaphase, the arrangement of the sister chromatids is obvious in monocentric chromosomes. In contrast, the sister chromatids of holocentric chromosomes cannot be distinguished even at mitotic metaphase. To clarify their organization, we differentially labelled the sister chromatids of holocentric Luzula and monocentric rye chromosomes by incorporating the base analogue EdU during replication. Using super-resolution structured illumination microscopy (SIM) and 3D rendering, we found that holocentric sister chromatids attach to each other at their contact surfaces similar to those of monocentrics in prometaphase. We found that sister chromatid exchanges (SCEs) are distributed homogeneously along the whole holocentric chromosomes of Luzula, and that their occurrence is increased compared to monocentric rye chromosomes. The SCE frequency of supernumerary B chromosomes, present additionally to the essential A chromosome complement of rye, does not differ from that of A chromosomes. Based on these results, models of the sister chromatid arrangement in mono- and holocentric plant chromosomes are presented.
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5

Zhao, Jian, Shaobo Jin, and Shui Hao. "The substructural organization of the chromosome core (scaffold) in meiotic chromosomes of Trilophidia annulata." Genetical Research 64, no. 3 (1994): 209–15. http://dx.doi.org/10.1017/s0016672300032869.

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SummaryThe substructural organization of chromosome cores or nonhistone proteins was studied within intact metaphase chromosomes at the second meiotic division in the grasshopper Trilophidia annulata by silver staining as well as light microscopy and whole mount electron microscopy of squash chromosomes. Our results revealed that the metaphase II chromosome contains a longitudinal, helical coiling core structure. Probably the two last organizational levels of the core packaging are achieved by helical coiling. The core structure retains the morphological characteristics of the original metaphase chromosome, surrounded by a halo of dispersed materials, which may be composed mainly of nonhistone proteins. The kinetochore is found to be connected with the chromosome core. The present findings combined with our previous observations on the helical structure of metaphase II chromosomes suggest that the folding path of the internal core structure in metaphase chromosomes is consistent with the final helical arrangement of the chromosome itself. These observations also imply that in condensed metaphase chromosomes nonhistone protein may form a compact network structure with helical appearance, which extends throughout the entire chromosome.
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6

Harrison, C. J., E. M. Jack, T. D. Allen, and R. Harris. "Light and scanning electron microscopy of the same human metaphase chromosomes." Journal of Cell Science 77, no. 1 (1985): 143–53. http://dx.doi.org/10.1242/jcs.77.1.143.

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A technique has been developed to examine the same G-banded human metaphase chromosomes, first in the light microscope and then in the scanning electron microscope (SEM). A structural involvement in chromosome banding was confirmed by a positional correlation between the G-positive bands observed in the light microscope and the circumferential grooves between the quaternary coils of the metaphase chromosomes, observed in the SEM. In further support of this the regions between the grooves showed a positional relationship with the G-negative or reverse (R) bands. The examination of slightly extended metaphase chromosomes in the light microscope demonstrated that the G-banding pattern corresponded to that described by the Paris nomenclature for metaphase chromosomes. The arrangement of the circumferential grooves of the same chromosomes, observed in the SEM, was shown to relate to that described by the Paris nomenclature for prometaphase chromosomes. Therefore, using the SEM it is possible to demonstrate the details of prometaphase banding in metaphase chromosomes.
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7

Lukhtanov, Vladimir A. "Two types of highly ordered micro- and macrochromosome arrangement in metaphase plates of butterflies (Lepidoptera)." Comparative Cytogenetics 13, no. 1 (2019): 19–25. http://dx.doi.org/10.3897/compcytogen.v13i1.32614.

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In karyotype of many organisms, chromosomes form two distinct size groups: macrochromosomes and microchromosomes. During cell divisions, the position of the macro- and microchromosomes is often ordered within metaphase plate. In many reptiles, amphibians, birds, insects of the orthopteran family Tettigoniidae and in some plants, a so called “reptilian” type organization is found, with microchromosomes situated in the center of metaphase plate and with macrochromosomes situated at the periphery. An opposite, “lepidopteran” type is known in butterflies and moths (i.e. in the order Lepidoptera) and is characterized by macrochromosomes situated in the center and by microchromosomes situated at the periphery. The anomalous arrangement found in Lepidoptera was previously explained by holocentric organization of their chromosomes. Here I analyse the structure of meiotic metaphase I plates in ithomiine butterfly, Forbestraolivencia (H. Bates, 1862) (Nymphalidae, Danainae, Ithomiini) which has a clear “reptilian” organization, contrary to previous observations in Lepidoptera. In this species large bivalents (i.e. macrochromosomes) form a regular peripheral circle, whereas the minute bivalents (i.e. microchromosomes) occupy the center of this circle. The reasons and possible mechanisms resulting in two drastically different spatial chromosome organization in butterflies are discussed.
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8

Lukhtanov, Vladimir A. "Two types of highly ordered micro- and macrochromosome arrangement in metaphase plates of butterflies (Lepidoptera)." Comparative Cytogenetics 13, no. (1) (2019): 19–25. https://doi.org/10.3897/CompCytogen.v13i1.32614.

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Abstract:
In karyotype of many organisms, chromosomes form two distinct size groups: macrochromosomes and microchromosomes. During cell divisions, the position of the macro- and microchromosomes is often ordered within metaphase plate. In many reptiles, amphibians, birds, insects of the orthopteran family Tettigoniidae and in some plants, a so called "reptilian" type organization is found, with microchromosomes situated in the center of metaphase plate and with macrochromosomes situated at the periphery. An opposite, "lepidopteran" type is known in butterflies and moths (i.e. in the order Lepidoptera) and is characterized by macrochromosomes situated in the center and by microchromosomes situated at the periphery. The anomalous arrangement found in Lepidoptera was previously explained by holocentric organization of their chromosomes. Here I analyse the structure of meiotic metaphase I plates in ithomiine butterfly, Forbestra olivencia (H. Bates, 1862) (Nymphalidae, Danainae, Ithomiini) which has a clear "reptilian" organization, contrary to previous observations in Lepidoptera. In this species large bivalents (i.e. macrochromosomes) form a regular peripheral circle, whereas the minute bivalents (i.e. microchromosomes) occupy the center of this circle. The reasons and possible mechanisms resulting in two drastically different spatial chromosome organization in butterflies are discussed.
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9

Yacobi, Y. Z., H. Levanony, and M. Feldman. "An ordered arrangement of bivalents at first meiotic metaphase of wheat." Chromosoma 91, no. 5 (1985): 347–54. http://dx.doi.org/10.1007/bf00291006.

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10

Yacobi, Y. Z., H. Levanony, and M. Feldman. "An ordered arrangement of bivalents at first meiotic metaphase of wheat." Chromosoma 91, no. 5 (1985): 355–58. http://dx.doi.org/10.1007/bf00291007.

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