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1

Vemuri, Jyothi G. Suryanarayana Murthy G. Nagaraj M. Khalilullah. "CUSCUTA SPECIES MEDICINAL APPLICATIONS AND EFFECTS BASED ON THEIR EXTRACTIVE COMPOUNDS." Journal of Pharma Research 10, no. 2 (2021): 05–11. https://doi.org/10.5281/zenodo.6473040.

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ABSTRACT Cuscuta species are plays a very robust and important role in the medicinal applications, this plant stems and seeds have highly important medicinal values. Some research studies say those Indian tribes and other traditional communities are used this plant as purgative, carminatives and external application for skin diseases. And it had antiviral and anti cancerousvactivities. Cuscuta reflexa Roxb, is prevalent in various regions of Bangladesh. And extracts of the stem reportedly demonstrated anti-steroidogenic and antibacterial activities The various pharmacological activities of who
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Aurang Zeb, Muhammad, Abdul Wahab, Najeeb Ullah, Sunil Pandey, and Taj Muhammad. "Antibacterial and Antifungal Activities of Micromeria biflora (Leaves)." International Journal of Medicine and Biomedical Sciences 1, no. 1 (2015): 28–34. http://dx.doi.org/10.55530/ijmbiosnepal.v1i1.7.

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 Background: Herbal medicines have been practiced for thousands of centuries by tribes all over the world. We aim to study extraction, fractionation, antibacterial and antifungal activity of micromeriabiflora.
 Methodology: Sample was washed properly with de-ionized water, solvents used (i.e. chloroform, ethyl acetate, n-hexane and methanol) were filtered and added. Some amounts of dehydrated reagents to purify the solvents before using. Shad dried plant powder was chopped and soaked in methanol for 10 days Dimethyl sulfoxide(DMSO) was used as solvent because it did not show any act
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3

Chukwuma, Michael, Uchenna E. Odoh, Christopher O. Ezugwu, Theodora C. Mba, and Austin Okorie. "Evaluation of gastroprotective and phytochemical properties of Terminalia superba engl. & diels (combretaceae) stem bark methanol extract." Journal of Current Biomedical Research 2, no. 6, November-December (2022): 600–616. http://dx.doi.org/10.54117/jcbr.v2i6.3.

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ABSTRACT Peptic ulcer is a disease that is affecting a large part of the population world-wide irrespective of the race and tribe. Advances made in the search for an ideal or perfect antiulcer drug has been eroded by cost, side effects and treatment failure. Search for cheap, safe, and effective drugs continues. It is the aim of the research to identify the secondary metabolites present in Terminalia superba Engl. & Diels (Combretaceae) stem bark methanol extract and investigate its gastroprotective properties. Methanol extract of T. superba was prepared by cold maceration extraction metho
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Murtiningsih, Titin Murtiningsih, Risa Supriningrum, and Henny Nurhasnawati. "IDENTIFICATION AND DETERMINATION OF SAPONIN CONTENT FROM EXTRACT OF Embelia borneensis BARK." AL ULUM: JURNAL SAINS DAN TEKNOLOGI 9, no. 3 (2023): 117. http://dx.doi.org/10.31602/jst.v9i3.12499.

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Abstract Embelia borneensis known sekilang used by the Dayak Kenyah tribe of Long Temuyat Village, Malinau, North Kalimantan as a fish poison and leech repellent. Sekilang bark extract contains alkaloids, tannins, saponins and flavonoids compounds. The purpose of this study was to determine the type and content of saponins in the methanol extract based on different maceration, reflux, and Soxhlet extraction methods. Saponin content was determined by the gravimetric method. The results showed that the type of saponin contained in sekilang bark extract was triterpenoid saponin. The average sapon
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Nitya Praptiwi, Nitya Praptiwi, and Bertha Mangallo. "Antidiabetic Activity of Methanol Extract of Bark of Alstonia scholaris from Papua." Jurnal Natural 18, no. 2 (2022): 67–74. http://dx.doi.org/10.30862/jn.v18i2.187.

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One of the biological wealth that grows in Papua is Alstonia scholaris, known by the Maybrat tribe as the swe plant and is used as a traditional medicine for antidiabetic. The Swe tree contains a lot of white sap and tastes very bitter. There is also a bitter taste in the roots, bark, and leaves so it is thought to contain a lot of bioactive compounds of alkaloids which have the potential as antidiabetic. This study aims to extract and determine of antidiabetic activity by the inhibition activity of the α-glucosidase of methanol extract of bark of Alstonia scholaris from Papua as a basis for t
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Ralte, Andrew Lalthasanga, Phaibiang Lapasam, Freddy Teilang Nongkhlaw, Pdiangmon Kyndait, and Zothanpuia. "Antioxidant and Analgesic Activity of Acer laevigatum Wall, a Traditional Mizo-tribe Medicine." International Journal of Pharmaceutical Sciences and Nanotechnology 13, no. 6 (2020): 5200–5207. http://dx.doi.org/10.37285/ijpsn.2020.13.6.4.

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Acer laevigatum is an evergreen tree growing to a height of 10–15 m or more, with a trunk up to 50 cm diameter belonging to the family Sapindaceae. In Mizoram, the decoction of the leaves is used as an external application in sprains. Extraction was carried out by drying the leaves and barks and extracted by using methanol as solvent using the Soxhlet apparatus. Preliminary phytochemical screening was carried out by methanolic extract of both leaves and barks to determine the chemical constituents present in the plant using a different phytochemical test, acute toxicity for leave and bark extr
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7

Nguyen, Minh Nhat, Thanh Dat Le, Bao Viet Nguyen, Thi Ngoc Lan Nguyen, Daniel Pioch, and Huynh Cang Mai. "Purification trials of Tamanu (Calophyllum inophyllum L.) oil." OCL 28 (2021): 53. http://dx.doi.org/10.1051/ocl/2021042.

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Tamanu (Calophyllum inophyllum L.) oil is a non-food oil used in traditional medicine, and with potential applications in the pharmaceutical and cosmetic industry. However, this oil, obtained by pressing the nuts, is being used as crude oil, in spite of a variable but large amount of non-lipids (called resin) being entrained. Although these should not be seen as impurities owing to their known bioactivity in many fields, not only they are responsible for the poisonous nature impeding human consumption in addition to bad smell, but they contribute to the poor oil quality, especially low stabili
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Reber, Eleanora A. "Comparison of Neutral Compound Extraction from Archaeological Residues in Pottery Using Two Methodologies: A Preliminary Study." Separations 8, no. 1 (2021): 6. http://dx.doi.org/10.3390/separations8010006.

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This study compares chloroform/methanol extraction and acidic methanol extraction of neutral compounds in absorbed lipid pottery residues from fourteen archaeological sherds. Previous studies have established that fatty acid extraction is more effective with acidic methanol extraction. This study suggests that acidic methanol extraction of neutral compounds, including sterols, alkanols, alkanes, and terpenoids, is more effective than or comparable to chloroform/methanol solvent extraction in most cases. The acidic methanol method extracts sterols, terpenoids, and alkanes more effectively than
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9

Liu, Shihong, Husam A. Abu Hajar, Guy Riefler, and Ben J. Stuart. "Lipid Extraction fromSpirulinasp. andSchizochytriumsp. Using Supercritical CO2with Methanol." BioMed Research International 2018 (December 9, 2018): 1–12. http://dx.doi.org/10.1155/2018/2720763.

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Microalgae are one of the most promising feedstocks for biodiesel production due to their high lipid content and easy farming. However, the extraction of lipids from microalgae is energy intensive and costly and involves the use of toxic organic solvents. Compared with organic solvent extraction, supercritical CO2(SCCO2) has demonstrated advantages through lower toxicity and no solvent-liquid separation. Due to the nonpolar nature of SCCO2, polar organic solvents such as methanol may need to be added as a modifier in order to increase the extraction ability of SCCO2. In this paper, pilot scale
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Gines, Brandon, Willard Collier, Mohamed Abdalla, and Teshome Yehualaeshet. "Influence of Metabolite Extraction Methods on 1H-NMR-Based Metabolomic Profiling of Enteropathogenic Yersinia." Methods and Protocols 1, no. 4 (2018): 45. http://dx.doi.org/10.3390/mps1040045.

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Metabolite extraction is one of the critical steps in microbial metabolome analysis. It affects both the observed metabolite content and biological interpretation of the data. Several methods exist for metabolite extraction of microbes, but the literature is not consistent regarding the sample model, adequacy, and performance of each method. In this study, an optimal extraction protocol for Yersinia intracellular metabolites was investigated. The effect of five extraction protocols consisting of different extraction solvent systems (60% methanol, 100% methanol, acetonitrile/methanol/water (2:2
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11

Wood, Lindsay W. "Chloroform–Methanol Extraction of Chlorophyll a." Canadian Journal of Fisheries and Aquatic Sciences 42, no. 1 (1985): 38–43. http://dx.doi.org/10.1139/f85-005.

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Chloroform–methanol (2:1, v/v) extracts significantly more (P < 0.001) chlorophyll a from algal cultures and from freshwater and seawater microcosms than does dimethyl sulfoxide, methanol, absolute methanol with hydrogen sulfide, or 90% acetone. For chlorophyte cultures it yields 97% or more of the chlorophyll a within a 4-h steeping period without grinding. It can be used with both calibrated spectrophotometry and fluorometric instrumentation. Sample filtration onto MgCO3-coated filters is recommended to ensure buffering of the extraction mixture. Holding the filters in chloroform–methanol
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12

NOUREEN, Shazia, Sobia NOREEN, Shazia Akram GHUMMAN, et al. "Seeds of Giant Dodder (Cuscuta reflexa) as a Function of Extract Procedure and Solvent Nature." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 46, no. 2 (2018): 653–62. http://dx.doi.org/10.15835/nbha46211088.

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Seeds of a renowned medicinal plant, giant dodder (Cuscuta reflexa), were assessed to appraise the effect of solvent and extraction technique variation on antioxidants potential. Dodder seed, also called cuscuta seed, has been considered superb tonic in traditional herbal medication for eyes, liver, spleen and kidney. Results show that selected solvent and procedure plays a key role in the composition and activity of extractable material. Three extraction procedures Orbital shaker, Decoction and Ultrasonic assisted extraction and five different solvents n-hexane, ethyl acetate, 100% methanol,
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13

Sela, Kong, Wiratni Budhijanto, and Arief Budiman. "Protein Extraction from Spirulina platensis by Using Ultrasound Assisted Extraction: Effect of Solvent Types and Extraction Time." Key Engineering Materials 872 (January 2021): 33–37. http://dx.doi.org/10.4028/www.scientific.net/kem.872.33.

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Protein is a substantial nutrition that essentially required by human. Spirulina platensis (Spp), well known as protein source could be a significant source to be used for many industrial applications. This study was investigated the effectiveness of ultrasound assisted extraction (UAE) method for protein extraction from Spp at various composition of solvent mixture and extraction time. Ethanol and mixture of methanol-ethanol were used as solvent. Extraction was conducted by varying ratios of solvent to biomass at 10:1, 12.5:1, and 15:1 (v/w), and extraction time (20, 35, and 50 min). Optimum
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Sela, Kong, Wiratni Budhijanto, and Arief Budiman. "Protein Extraction from Spirulina platensis by Using Ultrasound Assisted Extraction: Effect of Solvent Types and Extraction Time." Key Engineering Materials 872 (January 2021): 33–37. http://dx.doi.org/10.4028/www.scientific.net/kem.872.33.

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Protein is a substantial nutrition that essentially required by human. Spirulina platensis (Spp), well known as protein source could be a significant source to be used for many industrial applications. This study was investigated the effectiveness of ultrasound assisted extraction (UAE) method for protein extraction from Spp at various composition of solvent mixture and extraction time. Ethanol and mixture of methanol-ethanol were used as solvent. Extraction was conducted by varying ratios of solvent to biomass at 10:1, 12.5:1, and 15:1 (v/w), and extraction time (20, 35, and 50 min). Optimum
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15

Makić, Halid, Samira Hotić, Elvisa Hodžić, et al. "INFLUENCE OF MACERATION,SOLVENT TYPES,AND EXTRACTION DURATIONS ON THE YIELD OF MILK THISTLE SEEDS(SILYBUM MARIANUM)EXTRACTION." Technologica acta 17, no. 1 (2024): 67–76. http://dx.doi.org/10.51558/2232-7568.2023.17.1.67.

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The study investigated the extraction yield of defatted Silybum marianum seed samples using maceration as the sole extraction technique. Different solvent types (methanol, ethanol, and water) and extraction durations were tested. Prior to extraction, the samples were ground and defatted with n-hexane. For each combinationofsolvent type, and extraction duration, the extracted mass (g of extract/g of defatted sample) was determined. The impact of each parameter on the yield was analyzed, revealing significant effects.Results showed that water-based maceration for 4 hours yielded the highest aver
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16

Riyadi, P. H., E. Susanto, A. D. Anggo, M. H. Arifin, and L. Rizki. "Effect of methanol solvent concentration on the extraction of bioactive compounds using ultrasonic-assisted extraction (UAE) from Spirulina platensis." Food Research 7, Supplementary 3 (2023): 59–66. http://dx.doi.org/10.26656/fr.2017.7(s3).9.

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Spirulina platensis is a blue-green algae that contains bioactive compounds as a source of antioxidants. Methanol is a polar solvent; it has similarities to the content of the bioactive compound Spirulina platensis, which is polar. This study aimed to determine the effect of different concentrations of methanol solvent (50%, 70%, 96%) using UAE on the content of bioactive antioxidant compounds from Spirulina platensis. The parameters observed were yield, antioxidant activity, phytochemical screening, and GC-MS. The results showed that the difference in the concentration of methanol solvent usi
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17

WU, PEI-YIN, YUE-HORNG YEN, and ROBIN Y. Y. CHIOU. "Extraction of Aflatoxin from Peanut Meal and Kernels by CO2-Methanol Using a Highly Pressurized Fluid Extraction Process." Journal of Food Protection 58, no. 7 (1995): 800–803. http://dx.doi.org/10.4315/0362-028x-58.7.800.

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Peanut kernels infected with Aspergillus parasiticus after milling into meals were used as toxin-contaminated material for aflatoxin extraction using CO2–methanol. The percentage of aflatoxin recovery increased with CO2–60% methanol and decreased with CO2–100% methanol with an increase in the methanol/peanut meal ratio. One gram of peanut meal and 6 ml of 60% methanol were deposited in an extraction chamber (ca. 13 ml), pressurized with CO2 to 3,000 psi at 50°C, and held for 15 min, followed by releasing and collecting the extraction medium. The extracted solution was applied to an affinity co
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Kanda, Hideki, Kaito Kusumi, Li Zhu та Tao Wang. "Direct Extraction of Lipids, β-Carotene, and Polyphenolic Compounds from Wet Microalga Dunaliella salina by Liquefied Dimethyl Ether". Marine Drugs 22, № 10 (2024): 438. http://dx.doi.org/10.3390/md22100438.

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Extraction of lipids and high-value products from highly wet microalgae requires significant energy for the drying pretreatment. In this study, we examined the direct extraction of lipids, β-carotene, and polyphenolic compounds from wet Dunaliella salina using liquefied dimethyl ether (DME), which is effective in lipid extraction for biofuel production. The amount of DME-extracted β-carotene was 7.0 mg/g, which was higher than that obtained from the chloroform–methanol extraction. Moreover, the total phenolic content extracted with DME and its antioxidant capacity were slightly higher than tho
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Ngibad, Khoirul, Muhammad Sungging Pradana, Juli Afifah, and Aulia Afiatunnisa. "Optimization of yellow passion fruit peel extraction method using methanol solvent to increase antioxidant activity." Jurnal Pijar Mipa 18, no. 1 (2023): 77–83. http://dx.doi.org/10.29303/jpm.v18i1.4434.

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This study aims to determine the concentration of methanol solvent, the temperature, and the time of the shaker in the best extraction. It can produce the best antioxidant activity of the peel of the yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg) using the DPPH method. The maceration process is carried out using methanol solvents. The filtrate is concentrated with a rotary evaporator until a concentrated extract is obtained. Variations in methanol concentrations include 60, 80, and 100% (v/v) in methanol-water solvent mixtures. Shaker time variations in the extraction process
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Ashraf-Khorassani, Mehdi, Larry T. Taylor, and Frank K. Schweighardt. "Comparison of Supercritical CHF3 and CO2 and Methanol-Modified CHF3 and CO2 for Extraction of Sulfonamides from Chicken Liver." Journal of AOAC INTERNATIONAL 79, no. 5 (1996): 1043–49. http://dx.doi.org/10.1093/jaoac/79.5.1043.

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Abstract Supercritical CHF3 and methanol-modified CHF3 were compared with supercritical CO2 and methanol- modified CO2 for extraction of sulfonamides from fortified chicken liver admixed with Hydromatrix. Results showed that solvating power and selectivity were higher for supercritical and methanolmodified CHF3 than for supercritical and methanolmodified C02. Visual observation showed that chicken liver extract obtained with methanol-modified CHF3 was cleaner than that obtained with methanol-modified C02. Fat precipitated in the solvent trap when CO2 was used as the extraction medium. Also, si
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Satrianegara, Fais, Nurshalati Tahar, Rusmadi Rukmana, et al. "The Effect of Various Extraction Methods and Solvents on the Phytochemical Contents and Antioxidant Capacities of Safflower Florets (Carthamus Tinctorius L.) from South Sulawesi." Trends in Sciences 21, no. 7 (2024): 7576. http://dx.doi.org/10.48048/tis.2024.7576.

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Safflower is a traditional medicine commonly applied for the treatment of varicella in South Sulawesi. The aim of this study is to study the effect of extraction methods and solvents on phytochemical content and antioxidant capacity in the extraction of safflower. The study began with sample extraction with various solvents, i.e., water, ethanol 70, methanol, ethyl acetate and hexane and various methods, i.e., reflux, Soxhlet, hot maceration, cold maceration and room temperature maceration. Each extract was standardized for its phytochemical content, i.e., phenolics, flavonoids and carotenoids
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Ahmetović, Emina, Aida Smajlagić, Samir Ćurić, et al. "Evaluation of Different Extraction Techniques and Solvents on Yield of Polyphenolic Compounds from Pomegranate Peel (lat. Punica granatum)." Journal of Scientific Research and Reports 31, no. 5 (2025): 773–83. https://doi.org/10.9734/jsrr/2025/v31i53072.

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In this study, polyphenolic compounds from pomegranate peel (Punica granatum) were extracted using different extraction methods. Three techniques were applied for polyphenol extraction: Soxhlet extraction, ultrasound-assisted extraction, and maceration. These methods varied in the time required for extraction and the yield of dry extract. For Soxhlet and ultrasound-assisted extraction, two solvents were used: methanol and ethanol. While maceration is simple and cost-effective, it was found to be the least efficient method for extraction. The removal of ethanol and methanol from the extracts wa
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Bat Özmatara, Merve, and Şule Dinç Zor. "Achievement of antioxidants from Abelmoschus esculentus L. seeds using ultrasound-assisted extraction optimized by the help of experimental design approach." Journal of Chemical Metrology 14, no. 2 (2020): 160–68. http://dx.doi.org/10.25135/jcm.49.20.07.1742.

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Developments of food supplements and nutraceuticals from different natural origins require the use of some extraction methods. The success of the procedure relies on theefficiency of the extraction methods. Therefore, extraction methods need to be optimized. This work aimed for the determination of optimal conditions of ultrasonic-assisted extraction (UAE) of antioxidant constituents from okra (Abelmoschus esculentus L.) seeds which contain high contents of polysaccharides, polyphenols and flavonoids using response-surface methodology based on Box-Behnken experimental design (BBD). The indepen
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Bowles, N. Dean, Hans W. Paerl, and Jane Tucker. "Effective Solvents and Extraction Periods Employed in Phytoplankton Carotenoid and Chlorophyll Determinations." Canadian Journal of Fisheries and Aquatic Sciences 42, no. 6 (1985): 1127–31. http://dx.doi.org/10.1139/f85-139.

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Using high-performance liquid chromatography (HPLC), we showed that spectrophotometric determinations of algal lipophilic pigments are often biased by the choice of extraction solvent. Several commonly used extraction solvents and extraction periods were tested on diverse phytoplankton species isolated from the Neuse River, NC. Extraction solvents included 100% acetone, 90% acetone – 10% distilled deionized water (DDW), 50% acetone – 50% methanol, and 90% methanol – 10% DDW. Extraction efficiencies varied significantly among genera. For the diatom Cyclotella meneghiniana, at least three solven
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Tsirigka, Asimina, Maria Ntoula, Konstantinos N. Kontogiannopoulos, Anastasios J. Karabelas, and Sotiris I. Patsios. "Optimization of Solvent Extraction of Lipids from Yarrowia lipolytica towards Industrial Applications." Fermentation 9, no. 1 (2022): 35. http://dx.doi.org/10.3390/fermentation9010035.

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Extraction of intracellular lipids of the oleaginous yeast Yarrowia lipolytica has been systematically studied aiming towards a sustainable extraction process for lipid recovery. Selection of suitable industrial (bulk) solvents and extraction parameters that lead to maximization of lipid recovery are significant issues to be addressed, with industrial applications motivating this study. Biomass from fermentation of Yarrowia lipolytica (MUCL 28849) was used in small laboratory tests to assess different solvent mixtures (i.e., methanol/hexane, isopropanol/hexane, and methanol/ethyl acetate), imp
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Dewi, Kurnia Harlina, Markom Masturah, and Wan Ramli Wan Daud. "Parameter Optimization in the Extraction of Sea Cucumber (Holothuria scabra j) as a Source of Testosterone." Advanced Materials Research 233-235 (May 2011): 1358–65. http://dx.doi.org/10.4028/www.scientific.net/amr.233-235.1358.

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Testosterone, the steroid hormone used in sex reversal and as an aphrodisiac, is produced not only by sea cucumber, but many other organisms too. This research was aimed at optimizing conventional extraction of testosterone from sea cucumber, looking at method, type of solvent, solvent ratio, and temperature for isolation. The results showed that extraction by reflux produced the highest testosterone content, followed by soxhlet extraction. The lowest yield was produced by maceration extraction. The solvents selected were acetone, methanol, methanol/chloroform mixture (1:2) and chloroform. The
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Chutrtong, Jaruwan, and Kunyanuth Kularbphettong. "Study on optimal conditions of lemongrass extraction." E3S Web of Conferences 100 (2019): 00008. http://dx.doi.org/10.1051/e3sconf/201910000008.

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This research was conducted to find the optimal conditions to extract active ingredients from lemongrass for using to inhibit microorganisms. The solvents selected for testing are safe chemicals such as water, ethanol and methanol. We also studied the ratio of the lemongrass and the extract solvent and extract time which make the most extraction. Using water as an extract solvent, whether it is heating or not, the extract cannot inhibit the microbes. While using ethanol and methanol as extract solvent, no heat, the extract showed effectiveness in microorganisms inhibition at lemongrass extract
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Heal, Hussein Hamed, Zainab Tuama Al-Dallee, and Enas J. Khadim. "Extraction, Isolation and Identification of Luteolin Flavonoid from Vitex pseudonegundo leaves." IOP Conference Series: Earth and Environmental Science 1262, no. 5 (2023): 052016. http://dx.doi.org/10.1088/1755-1315/1262/5/052016.

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Abstract Objective: Using HPLC as a quantitative estimate technique, the optimal extraction solvent for luteolin from Vitex pseudo-negundo was identified. Methods: leaves V. pseudonegundo were gathered, washed, and dried. employeing methanol and ethanol as the extraction solvents (both with varied strengths), as well as the extraction techniques of maceration, reflux, and Soxhlet. The amount of luteolin in each sample extract was measured using a preparative HPLC technique Results: Methanol was the best solvent, and the best extraction method was the reflex extraction method, based on HPLC res
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Jeffus, Marshall T., and James G. Stewart. "Formulas for Calculation of Extraction Volumes for Commonly Used Pesticide Residue Extraction Procedures." Journal of AOAC INTERNATIONAL 68, no. 3 (1985): 437–39. http://dx.doi.org/10.1093/jaoac/68.3.437.

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Abstract Formulas are presented for the calculation of extraction volume for pesticide residue procedures that use a single extraction with acetone, acetonitrile, or methanol, with or without prior dilution of these solvents with water. These formulas account for the volume change on mixing and for the volume contribution from the soluble sugars sucrose, glucose, and fructose. Results using these formulas are in agreement with the observed volumes of such mixtures within 0.3% throughout the following ranges, expressed as percent water in the mixtures: 15- 38% for acetone, 4.8-59% for acetonitr
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Lin, Li Jing, Xiao Bing Huang, Ji Hua Li, Xu Ran, and Yong Fu Tang. "Ultrasonic Extraction of Phenolics from Longan Seed by Response Surface Methodology." Advanced Materials Research 634-638 (January 2013): 1302–8. http://dx.doi.org/10.4028/www.scientific.net/amr.634-638.1302.

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A Box-Behnken experimental design and response surface methodology (RSM) was employed to optimize the parameters of ultrasonic extraction of phenolics from longan seed based on the single-factor experiments. The effects of extraction temperature, methanol concentration and extraction time on the extraction yield of phenolics from longan seed were investigated. The major parameter influencing the extraction was the extraction temperature. In this experiment, the maximal extraction yield of phenolics was obtained with 5.931 ± 0.016% (n = 3) at extraction temperature 82°C, methanol concentration
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Petreska Stanoeva, Jasmina, Elena Balshikevska, Marina Stefova, Oliver Tusevski, and Sonja G. Simic. "Comparison of the Effect of Acids in Solvent Mixtures for Extraction of Phenolic Compounds From Aronia melanocarpa." Natural Product Communications 15, no. 7 (2020): 1934578X2093467. http://dx.doi.org/10.1177/1934578x20934675.

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Thirty-two different solvent mixtures containing methanol and acid (acetic, formic, and hydrochloric); methanol, water, and acid; and pure methanol were tested for their efficiency for extraction of phenolic compounds from aronia ( Aronia melanocarpa) belonging to the groups of anthocyanins, flavonols, and hydroxycinnamic acid derivatives. Thirteen compounds were detected and quantified in the extracts using HPLC/DAD/ESI-MS n. The yield of each compound and group was evaluated with regard to the extraction solvent composition. Extraction mixtures containing HCl were superior to the ones contai
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Patel, Geetha, Harshita M., Sathi D., and Padmaa M. Paarakh. "ANTICANCER STUDIES ON THE LEAVES OF TINOSPORA CORDIFOLIA (WILD) MIERS." International Journal of Advanced Research 10, no. 10 (2022): 1029–35. http://dx.doi.org/10.21474/ijar01/15578.

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The aim of the study was to evaluate anticancer activity of the methanol and aqueous extracts of leaves of Tinosporacordifolia Wild Miers against a Human Breast Carcinoma Cell line(MDAMB231) by MTT assay. The coarse powder of leaf were subjected to Soxhletextraction and microwave assisted extraction using methanol and water as solvent. The IC50 values of aqueous extract of leaf followed by soxhlation and methanol extract of leaf by microwave extraction were found to be 59.85μg/mL and 249μg/mLrespectively in MDAMB231 cells in comparison to Standard Vincristine 14.41µM whereas methanol extrac
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Baeshen, Nabih A., Yaaser Q. Almulaiky, Mohamed Afifi, et al. "GC-MS Analysis of Bioactive Compounds Extracted from Plant Rhazya stricta Using Various Solvents." Plants 12, no. 4 (2023): 960. http://dx.doi.org/10.3390/plants12040960.

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Worldwide, human beings have traditionally employed many folkloric herbal resources as complementary and alternative remedies, and these remedies have played a pivotal role in modern medicines for many decades, as scientists have used them to develop drugs. We studied the effects of employing solvents with varying polarity on the yields of phytochemical components extracted from the plant Rhazya stricta. We used chloroform–methanol (1:1), methanol, ethanol, diethyl ether, and ethyl acetate as extraction solvents. The results showed that the efficiencies of the solvents at extracting phytochemi
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Toqeer Ahmed. "Between Past and Present: Unravelling Colonialism’s Enduring Impact in The Wandering Falcon." Journal of Contemporary Poetics 7, no. 1 (2023): 15–31. http://dx.doi.org/10.54487/jcp.v7i1.3103.

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In my exploration of the profound and enduring impacts of colonialism on the nomadic tribes of Baluchistan and the tribal areas of Pakistan, I draw from Jamil Ahmad’s The Wandering Falcon and insights from postcolonial theorists. My study illuminates the tribes’ persistent struggles for recognition, representation, and basic rights. These tribes, once proud custodians of their distinct cultural heritage, now confront challenges from modern encroachments, political marginalisation, and economic hardships, all deeply rooted in colonial legacies. The imposition of foreign legal systems, combined
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Afandi, Asrul, Shazani Sarijan, and Ranajit Kumar Shaha. "Optimization of Rebaudioside A Extraction from Stevia Rebaudiana (Bertoni) and Quantification by High Performance Liquid Chromatography Analysis." Journal of Tropical Resources and Sustainable Science (JTRSS) 1, no. 1 (2021): 62–70. http://dx.doi.org/10.47253/jtrss.v1i1.671.

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A solid-liquid extraction and an HPLC method for determination of rebaudioside A from the leave parts of Stevia rebaudiana were developed. Separation method consisted of solvent extraction of leaf powder using various solvents like petroleum ether, methanol, diethyl ether and butanol followed by its purification using high performance liquid chromatography in order to obtain bioactive compound rebaudioside A. This solvent selection is very important prior to alternative extraction methods since it can be used as a pre-extraction solvents, main solvents, or co-solvents. The problem of hydrolysa
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Zenati, Ruba A., Alexander D. Giddey, Hamza M. Al-Hroub, et al. "Evaluation of Two Simultaneous Metabolomic and Proteomic Extraction Protocols Assessed by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry." International Journal of Molecular Sciences 24, no. 2 (2023): 1354. http://dx.doi.org/10.3390/ijms24021354.

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Untargeted multi-omics analysis of plasma is an emerging tool for the identification of novel biomarkers for evaluating disease prognosis, and for developing a better understanding of molecular mechanisms underlying human disease. The successful application of metabolomic and proteomic approaches relies on reproducibly quantifying a wide range of metabolites and proteins. Herein, we report the results of untargeted metabolomic and proteomic analyses from blood plasma samples following analyte extraction by two frequently-used solvent systems: chloroform/methanol and methanol-only. Whole blood
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Senevirathna, Nuwanthi, Morteza Hassanpour, Ian O’Hara, and Azharul Karim. "Sustainable Extraction of Fresh Banana Inflorescence by ASE: Optimization and Characterization of Anthocyanin Rich Extracts by LC-UV-MS/MS." Foods 14, no. 8 (2025): 1299. https://doi.org/10.3390/foods14081299.

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Sustainable and environmentally friendly extraction methods for natural bioactive compounds are gaining significant attention in the food, beverage, and nutraceutical industries. Among these bioactive compounds, anthocyanins, which are potent antioxidants, have garnered particular interest due to their health-promoting properties. Banana inflorescence, an underutilized agricultural by-product, is a rich source of bioactive compounds. However, the extraction of bioactive compounds is often energy-intensive, which raises concerns about environmental sustainability. Accelerated solvent extraction
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Mohammad Azmin, Siti Nuurul Huda, Nor Alafiza Yunus, Sharifah Rafidah Wan Alwi, and Mohd Shukri Mat Nor. "Stability Analysis of Binary Solvent Mixtures for Herbal Phytochemical Extraction." Journal of Tropical Resources and Sustainable Science (JTRSS) 5, no. 3 (2017): 128–33. http://dx.doi.org/10.47253/jtrss.v5i3.657.

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Most of the extraction processes of herbal phytochemicals use solvent mixtures as a phytochemical transfer mediums. It is very important to predict the stability of solvent mixtures before it is used to extract herbal phytochemicals. In order to prevent any disturbance in the herbal extraction, the solvent mixtures must be in a single liquid phase (miscible to each other). In this study, the stabilities of five binary solvents (methanol-water, methanol-ethyl acetate, methanol-acetic acid, methanol-n-propionaldehyde, and methanol-isobutyraldehyde mixtures) that could be used in the current extr
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Major, Nikola, Bernard Prekalj, Josipa Perković, Dean Ban, Zoran Užila, and Smiljana Ban. "The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile." Plants 9, no. 12 (2020): 1792. http://dx.doi.org/10.3390/plants9121792.

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The extraction of glucosinolates in boiling aqueous methanol from freeze dried leaf tissues is the most common method for myrosinase inactivation but can be hazardous because of methanol toxicity. Although freeze drying is the best dehydration method in terms of nutritional quality preservation, the main drawbacks are a limited sample quantity that can be processed simultaneously, a long processing time, and high energy consumption. Therefore, the aim of this study is to evaluate the effects of applying high temperature for myrosinase inactivation via hot air drying prior to the extraction ste
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Ushie, O. A., B. D. Longbap, T. I. Azuaga, S. I. Iyen, D. I. Ugwuja, and R. F. Ijoko. "Phytochemical screening and proximate analysis of the leaf extracts of Vitex doniana." Scientia Africana 21, no. 1 (2022): 149–58. http://dx.doi.org/10.4314/sa.v21i1.13.

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The method of cold maceration was used in the extraction by serial exhaustive extraction method which involves successive extraction with solvents of increasing polarity from a nonpolar (hexane) to a more polar solvent (methanol) to ensure that a wide polarity range of compound could be extracted. The phytochemical screening of the crude extract of Vitex doniana showed that flavonoids, steroids, phlobatannins, alkaloids, saponins were found to be present. Alkaloids were detected in the methanol, n-Hexane, acetone and ethyl acetate extracts when Wagner`s reagent was used. Flavonoids were detect
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OZAKI, Jun-ichi, Subagijo KASTRIA INGWANG DJAJA, and Asao OYA. "Supercritical Methanol Extraction of Loy Yang Coal." Journal of the Japan Institute of Energy 80, no. 4 (2001): 274–77. http://dx.doi.org/10.3775/jie.80.274.

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Mittova, Valentina, Marina Pirtskhalava, Roza Bidzinashvili, Malkhaz Vakhania, Tornike Mindiashvili, and Mariam Kobiashvili. "Effects of different drying, extraction methods, and solvent polarity on the antioxidant properties of Paeonia daurica subsp. mlokosewitschii leaves." Modern Issues of Medicine and Management 26, no. 2 (2023): 1–15. http://dx.doi.org/10.56580/geomedi39.

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The study presents the effect of drying methods (microwave-drying and freeze-drying), different solvents (80% methanol, 99% methanol, 80% ethanol), and different extraction methods on the antioxidant activity of Paeonia daurica subsp. mlokosewitschii leaves, estimated based on DPPH free radical scavenging activity. The highest antioxidant activity was revealed in the freeze-dried leaves. The drying method significantly influenced the antioxidant activity: the thermal microwave-drying resulted in lower antioxidant potential. The solvent polarity also played a significant role in the determinati
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43

V. González de Peredo, Ana, Mercedes Vázquez-Espinosa, Estrella Espada-Bellido, et al. "Alternative Ultrasound-Assisted Method for the Extraction of the Bioactive Compounds Present in Myrtle (Myrtus communis L.)." Molecules 24, no. 5 (2019): 882. http://dx.doi.org/10.3390/molecules24050882.

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The bioactive compounds in myrtle berries, such as phenolic compounds and anthocyanins, have shown a potentially positive effect on human health. Efficient extraction methods are to be used to obtain maximum amounts of such beneficial compounds from myrtle. For that reason, this study evaluates the effectiveness of a rapid ultrasound-assisted method (UAE) to extract anthocyanins and phenolic compounds from myrtle berries. The influence of solvent composition, as well as pH, temperature, ultrasound amplitude, cycle and solvent-sample ratio on the total phenolic compounds and anthocyanins conten
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Wang, Xiaokang, Yuanyuan Cui, Yingying Song, et al. "Studies on the Prediction and Extraction of Methanol and Dimethyl Carbonate by Hydroxyl Ammonium Ionic Liquids." Molecules 28, no. 5 (2023): 2312. http://dx.doi.org/10.3390/molecules28052312.

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The separation of dimethyl carbonate (DMC) and methanol is of great significance in industry. In this study, ionic liquids (ILs) were employed as extractants for the efficient separation of methanol from DMC. Using the COSMO-RS model, the extraction performance of ILs consisting of 22 anions and 15 cations was calculated, and the results showed that the extraction performance of ILs with hydroxylamine as the cation was much better. The extraction mechanism of these functionalized ILs was analyzed by molecular interaction and the σ-profile method. The results showed that the hydrogen bonding en
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Tesfaye, Bereket, Tilahun Tefera, Misikir O, and Tsegaye G. "EXTRACTION AND COMPARISON OF ESSENTIAL OIL FROM NEEM SEED BY USING SOXHLET EXTRACTION AND SIMPLE DISTILLATION METHODS." International Journal of Engineering Technologies and Management Research 5, no. 9 (2020): 74–81. http://dx.doi.org/10.29121/ijetmr.v5.i9.2018.291.

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Extraction of essential neem oil from neem seed were carried by soxhelt extraction and simple distillation method using different types of solvents and parameters. Physico-chemical characteristics of the extracted oil were also determined by using classical wet chemical method. Results were generated in both techniques. Soxhlet extraction using hexane has 40.35%, using ethanol-hexane mixture of 60:40% volume proportions have 43.71%, using ethanol 42.65% and using methanol 42.89%. In simple distillation method results reveal that using hexane 42.35%, in methanol 39.5%, using ethanol 40.72% and
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46

Bereket, Tesfaye, Tefera Tilahun, O. Misikir, and G. Tsegaye. "EXTRACTION AND COMPARISON OF ESSENTIAL OIL FROM NEEM SEED BY USING SOXHLET EXTRACTION AND SIMPLE DISTILLATION METHODS." International Journal of Engineering Technologies and Management Research 5, no. 9 (2018): 74–81. https://doi.org/10.5281/zenodo.1455798.

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Extraction of essential neem oil from neem seed were carried by soxhelt extraction and simple distillation method using different types of solvents and parameters. Physico-chemical characteristics of the extracted oil were also determined by using classical wet chemical method. Results were generated in both techniques. Soxhlet extraction using hexane has 40.35%, using ethanol-hexane mixture of 60:40% volume proportions have 43.71%, using ethanol 42.65% and using methanol 42.89%. In simple distillation method results reveal that using hexane 42.35%, in methanol 39.5%, using ethanol 40.72% and
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47

Zhang, Wen Yi, Ning Han, Li Rong Yao, Xiao Lan Qiu, and Xiao Liang Chen. "Study on the Extraction and Purification of Microcystins (MC-LR)." Advanced Materials Research 340 (September 2011): 318–23. http://dx.doi.org/10.4028/www.scientific.net/amr.340.318.

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The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high effi
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Cho, In-Ho, Eberhard Hohaus, Axel Lehnen, and Harro Lentz. "Extraktionen von Ginsenosiden aus Ginseng-Wurzeln mit flüssigem Ammoniak, Methanol-Wasser oder Wasser/ Extractions of Ginsenosidesj from Ginseng Roots with Liquid Ammonia, Methanol-Water or Water." Zeitschrift für Naturforschung B 55, no. 3-4 (2000): 326–32. http://dx.doi.org/10.1515/znb-2000-3-415.

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Abstract Extractions of ginsenosides from ginseng roots with liquid ammonia, methanol/water (60:40; v/v) or water were carried out. The extracts have been analyzed qualitatively and quantitatively to valuate yield and selectivity of extractions of ginsenosides. Water supplied the lowest yield. The yields of extracts with liquid ammonia were higher than those with m ethanol-water (60%). Yields of the ginsenosides Rb1, Rb2, Rc and Rd by extracting with liquid ammonia are about twice as much as those of the extraction with methanol-water (60%). It was proved by HPLC that malonyl-ginsenosides m -R
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TAYLOR, T. MATTHEW, P. MICHAEL DAVIDSON, and QIXIN ZHONG. "Extraction of Nisin from a 2.5% Commercial Nisin Product Using Methanol and Ethanol Solutions." Journal of Food Protection 70, no. 5 (2007): 1272–76. http://dx.doi.org/10.4315/0362-028x-70.5.1272.

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Nisin is a class Ia bacteriocin used widely in the food industry to inhibit a number of gram-positive pathogens. Although this peptide exhibits activity against many gram-positive bacteria, its effectiveness can vary significantly depending upon the food application. Encapsulation is one method that has been investigated for improving the activity of nisin. Improvement of the encapsulation efficiency of nisin requires purification of the compound, which can be accomplished utilizing organic solvents. The objective of this study was to use methanol and ethanol solutions to extract and concentra
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Lancaster, Sarah, Scott Senseman, and Katherine Carson. "Accelerated Solvent Extraction of Fluometuron from Selected Soils." Journal of AOAC INTERNATIONAL 90, no. 4 (2007): 1142–45. http://dx.doi.org/10.1093/jaoac/90.4.1142.

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Abstract Accelerated solvent extraction (ASE) is a recently developed extraction technique that is more rapid and produces less waste than do conventional liquid/liquid extraction methods. Optimal conditions were determined for ASE of fluometuron from 2 Weswood clay loam soils. Two solvents (acetonitrile and methanol), 2 temperatures (50 and 100C), and the number of static cycles (1, 2, and 3) were evaluated. The most efficient and reproducible extractions were obtained when methanol was combined with a 50C extraction temperature and the static cycle was repeated 3 times. These experiments ind
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