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Journal articles on the topic 'Methylotrophy'

Author: Grafiati

Published: 4 June 2021

Last updated: 7 July 2024

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1

Jourand, Philippe, Adeline Renier, Sylvie Rapior, et al. "Role of Methylotrophy During Symbiosis Between Methylobacterium nodulans and Crotalaria podocarpa." Molecular Plant-Microbe Interactions® 18, no. 10 (2005): 1061–68. http://dx.doi.org/10.1094/mpmi-18-1061.

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Some rare leguminous plants of the genus Crotalaria are specifically nodulated by the methylotrophic bacterium Methylobacterium nodulans. In this study, the expression and role of bacterial methylotrophy were investigated during symbiosis between M. nodulans, strain ORS 2060T, and its host legume, Crotalaria podocarpa. Using lacZ fusion to the mxaF gene, we showed that the methylotroph genes are expressed in the root nodules, suggesting methylotrophic activity during symbiosis. In addition, loss of the bacterial methylotrophic function significantly affected plant development. Indeed, inoculat

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2

Yanpirat, Patcha, Yukari Nakatsuji, Shota Hiraga, et al. "Lanthanide-Dependent Methanol and Formaldehyde Oxidation in Methylobacterium aquaticum Strain 22A." Microorganisms 8, no. 6 (2020): 822. http://dx.doi.org/10.3390/microorganisms8060822.

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Lanthanides (Ln) are an essential cofactor for XoxF-type methanol dehydrogenases (MDHs) in Gram-negative methylotrophs. The Ln3+ dependency of XoxF has expanded knowledge and raised new questions in methylotrophy, including the differences in characteristics of XoxF-type MDHs, their regulation, and the methylotrophic metabolism including formaldehyde oxidation. In this study, we genetically identified one set of Ln3+- and Ca2+-dependent MDHs (XoxF1 and MxaFI), that are involved in methylotrophy, and an ExaF-type Ln3+-dependent ethanol dehydrogenase, among six MDH-like genes in Methylobacterium

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3

Yurimoto, Hiroya, Masahide Oku, and Yasuyoshi Sakai. "Yeast Methylotrophy: Metabolism, Gene Regulation and Peroxisome Homeostasis." International Journal of Microbiology 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/101298.

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Eukaryotic methylotrophs, which are able to obtain all the carbon and energy needed for growth from methanol, are restricted to a limited number of yeast species. When these yeasts are grown on methanol as the sole carbon and energy source, the enzymes involved in methanol metabolism are strongly induced, and the membrane-bound organelles, peroxisomes, which contain key enzymes of methanol metabolism, proliferate massively. These features have made methylotrophic yeasts attractive hosts for the production of heterologous proteins and useful model organisms for the study of peroxisome biogenesi

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4

CastilloVillanueva, Elizabeth, Jorge Valdivia-Anistro, Ariadnna CruzCórdova, Valeria Souza, and Irma Rosas-Pérez. "Diversity of cultivated methylotrophs from the extremely oligotrophic system in the Cuatro Cienegas Basin, Mexico: An unexplored ecological guild." Journal of Microbiology & Experimentation 10, no. 6 (2022): 208–14. http://dx.doi.org/10.15406/jmen.2022.10.00375.

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The simplest form of heterotrophy in the carbon cycle is to metabolize C1 compounds, this is a widely spread strategy that includes genus in different phyla inhabiting diverse environments that seem to have acquired the methanol dehydrogenase by horizontal gene transfer (HGT). The objective of this study was to isolate and explore the diversity of the ecological guild of methylotrophs in the water and riparian vegetation of the Churince system in the Cuatro Cienegas Basin (CCB), Coahuila, Mexico. Methylotrophy was verified by polymerase chain reaction (PCR) amplification of the mxaF gene that

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5

Chistoserdova, Ludmila, Alla Lapidus, Cliff Han, et al. "Genome of Methylobacillus flagellatus, Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy." Journal of Bacteriology 189, no. 11 (2007): 4020–27. http://dx.doi.org/10.1128/jb.00045-07.

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ABSTRACT Along with methane, methanol and methylated amines represent important biogenic atmospheric constituents; thus, not only methanotrophs but also nonmethanotrophic methylotrophs play a significant role in global carbon cycling. The complete genome of a model obligate methanol and methylamine utilizer, Methylobacillus flagellatus (strain KT) was sequenced. The genome is represented by a single circular chromosome of approximately 3 Mbp, potentially encoding a total of 2,766 proteins. Based on genome analysis as well as the results from previous genetic and mutational analyses, methylotro

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6

Islam, Tajul, Marcela Hernández, Amare Gessesse, J. Colin Murrell, and Lise Øvreås. "A Novel Moderately Thermophilic Facultative Methylotroph within the Class Alphaproteobacteria." Microorganisms 9, no. 3 (2021): 477. http://dx.doi.org/10.3390/microorganisms9030477.

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Methylotrophic bacteria (non-methanotrophic methanol oxidizers) consuming reduced carbon compounds containing no carbon–carbon bonds as their sole carbon and energy source have been found in a great variety of environments. Here, we report a unique moderately thermophilic methanol-oxidising bacterium (strain LS7-MT) that grows optimally at 55 °C (with a growth range spanning 30 to 60 °C). The pure isolate was recovered from a methane-utilizing mixed culture enrichment from an alkaline thermal spring in the Ethiopia Rift Valley, and utilized methanol, methylamine, glucose and a variety of multi

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7

Klein, Vivien Jessica, Marta Irla, Marina Gil López, Trygve Brautaset, and Luciana Fernandes Brito. "Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy." Microorganisms 10, no. 2 (2022): 220. http://dx.doi.org/10.3390/microorganisms10020220.

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Formaldehyde metabolism is prevalent in all organisms, where the accumulation of formaldehyde can be prevented through the activity of dissimilation pathways. Furthermore, formaldehyde assimilatory pathways play a fundamental role in many methylotrophs, which are microorganisms able to build biomass and obtain energy from single- and multicarbon compounds with no carbon–carbon bonds. Here, we describe how formaldehyde is formed in the environment, the mechanisms of its toxicity to the cells, and the cell’s strategies to circumvent it. While their importance is unquestionable for cell survival

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8

Henriques, Ana C., Rui M. S. Azevedo, and Paolo De Marco. "Metagenomic survey of methanesulfonic acid (MSA) catabolic genes in an Atlantic Ocean surface water sample and in a partial enrichment." PeerJ 4 (October 6, 2016): e2498. http://dx.doi.org/10.7717/peerj.2498.

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Methanesulfonic acid (MSA) is a relevant intermediate of the biogeochemical cycle of sulfur and environmental microorganisms assume an important role in the mineralization of this compound. Several methylotrophic bacterial strains able to grow on MSA have been isolated from soil or marine water and two conserved operons,msmABCDcoding for MSA monooxygenase andmsmEFGHcoding for a transport system, have been repeatedly encountered in most of these strains. Homologous sequences have also been amplified directly from the environment or observed in marine metagenomic data, but these showed a base co

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9

Brautaset, Trygve, Øyvind M. Jakobsen, Michael C. Flickinger, Svein Valla, and Trond E. Ellingsen. "Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus." Journal of Bacteriology 186, no. 5 (2004): 1229–38. http://dx.doi.org/10.1128/jb.186.5.1229-1238.2004.

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ABSTRACT Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50°C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph. Here we describe the DNA sequence and characterization of a 19,167-bp circular plasmid, designated pBM19, isolated from B. methanolicus MGA3. Sequence analysis of pBM19 demonstrated the presence of the methanol dehydrogenase gene, mdh, which is crucial for methanol consumption in this bacterium. In addition, f

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10

Sy, Abdoulaye, Antonius C. J. Timmers, Claudia Knief, and Julia A. Vorholt. "Methylotrophic Metabolism Is Advantageous for Methylobacterium extorquens during Colonization of Medicago truncatula under Competitive Conditions." Applied and Environmental Microbiology 71, no. 11 (2005): 7245–52. http://dx.doi.org/10.1128/aem.71.11.7245-7252.2005.

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ABSTRACT Facultative methylotrophic bacteria of the genus Methylobacterium are commonly found in association with plants. Inoculation experiments were performed to study the importance of methylotrophic metabolism for colonization of the model legume Medicago truncatula. Competition experiments with Methylobacterium extorquens wild-type strain AM1 and methylotrophy mutants revealed that the ability to use methanol as a carbon and energy source provides a selective advantage during colonization of M. truncatula. Differences in the fitness of mutants defective in different stages of methylotroph

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11

Marx, Christopher J., Brooke N. O'Brien, Jennifer Breezee, and Mary E. Lidstrom. "Novel Methylotrophy Genes of Methylobacterium extorquens AM1 Identified by using Transposon Mutagenesis Including a Putative Dihydromethanopterin Reductase." Journal of Bacteriology 185, no. 2 (2003): 669–73. http://dx.doi.org/10.1128/jb.185.2.669-673.2003.

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ABSTRACT Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR). This mutant has a C1-defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (H4MPT)-dependent formaldehyde oxidation. These results suggest that this gene, dmrA, may encode dihydromethanopterin reductase, an activity analogous to that of DHFR that is required for the final ste

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12

Funk, Michael A. "Dawn of methylotrophy." Science 366, no. 6461 (2019): 69.6–70. http://dx.doi.org/10.1126/science.366.6461.69-f.

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13

Wackett, Lawrence P. "Methanotrophy and methylotrophy." Environmental Microbiology Reports 4, no. 1 (2012): 156–57. http://dx.doi.org/10.1111/j.1758-2229.2011.00321.x.

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14

Martinez-Gomez, N. Cecilia, Nathan M. Good, and Mary E. Lidstrom. "Methenyl-Dephosphotetrahydromethanopterin Is a Regulatory Signal for Acclimation to Changes in Substrate Availability in Methylobacterium extorquens AM1." Journal of Bacteriology 197, no. 12 (2015): 2020–26. http://dx.doi.org/10.1128/jb.02595-14.

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ABSTRACTDuring an environmental perturbation, the survival of a cell and its response to the perturbation depend on both the robustness and functionality of the metabolic network. The regulatory mechanisms that allow the facultative methylotrophic bacteriumMethylobacterium extorquensAM1 to effect the metabolic transition from succinate to methanol growth are not well understood. Methenyl-dephosphotetrahydromethanopterin (methenyl-dH4MPT), an early intermediate during methanol metabolism, transiently accumulated 7- to 11-fold after addition of methanol to a succinate-limited culture. This accum

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15

Chistoserdova, Ludmila, and Marina G. Kalyuzhnaya. "Current Trends in Methylotrophy." Trends in Microbiology 26, no. 8 (2018): 703–14. http://dx.doi.org/10.1016/j.tim.2018.01.011.

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16

Chistoserdova, Ludmila. "Modularity of methylotrophy, revisited." Environmental Microbiology 13, no. 10 (2011): 2603–22. http://dx.doi.org/10.1111/j.1462-2920.2011.02464.x.

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17

Zhang, Wenming, Ting Zhang, Sihua Wu, et al. "Guidance for engineering of synthetic methylotrophy based on methanol metabolism in methylotrophy." RSC Advances 7, no. 7 (2017): 4083–91. http://dx.doi.org/10.1039/c6ra27038g.

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Methanol represents an attractive non-food raw material in biotechnological processes from an economic and process point of view. It is vital to elucidate methanol metabolic pathways, which will help to genetically construct non-native methylotrophs.

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18

De Marco, Paolo. "Methylotrophy versus heterotrophy: a misconception." Microbiology 150, no. 6 (2004): 1606–7. http://dx.doi.org/10.1099/mic.0.27165-0.

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19

Hendrickson, Erik L., David A. C. Beck, Tiansong Wang, Mary E. Lidstrom, Murray Hackett, and Ludmila Chistoserdova. "Expressed Genome of Methylobacillus flagellatus as Defined through Comprehensive Proteomics and New Insights into Methylotrophy." Journal of Bacteriology 192, no. 19 (2010): 4859–67. http://dx.doi.org/10.1128/jb.00512-10.

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ABSTRACT In recent years, techniques have been developed and perfected for high-throughput identification of proteins and their accurate partial sequencing by shotgun nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS), making it feasible to assess global protein expression profiles in organisms with sequenced genomes. We implemented comprehensive proteomics to assess the expressed portion of the genome of Methylobacillus flagellatus during methylotrophic growth. We detected a total of 1,671 proteins (64% of the inferred proteome), including all the predicted essential proteins

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20

Fukala, Ivo, and Igor Kučera. "Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods." Molecules 29, no. 10 (2024): 2293. http://dx.doi.org/10.3390/molecules29102293.

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Polyhydroxyalkanoates (PHAs) are intracellular biopolymers that microorganisms use for energy and carbon storage. They are mechanically similar to petrochemical plastics when chemically extracted, but are completely biodegradable. While they have potential as a replacement for petrochemical plastics, their high production cost using traditional carbon sources remains a significant challenge. One potential solution is to modify heterotrophic PHA-producing strains to utilize alternative carbon sources. An alternative approach is to utilize methylotrophic or autotrophic strains. This article prov

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21

Le, Thien-Kim, Su-Bin Ju, Hyewon Lee, et al. "Biosensor-Based Directed Evolution of Methanol Dehydrogenase from Lysinibacillus xylanilyticus." International Journal of Molecular Sciences 22, no. 3 (2021): 1471. http://dx.doi.org/10.3390/ijms22031471.

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Methanol dehydrogenase (Mdh), is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy. Engineering of Mdh, especially NAD-dependent Mdh, has thus been actively investigated to enhance methanol conversion. However, its poor catalytic activity and low methanol affinity limit its wider application. In this study, we applied a transcriptional factor-based biosensor for the direct evolution of Mdh from Lysinibacillus xylanilyticus (Lxmdh), which has a relatively high turnover rate and low KM value compared to other wild-type N

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22

Breuer, Uta, Jörg-Uwe Ackermann, and Wolfgang Babel. "Accumulation of poly(3-hydroxybutyric acid) and overproduction of exopolysaccharides in a mutant of a methylotrophic bacterium." Canadian Journal of Microbiology 41, no. 13 (1995): 55–59. http://dx.doi.org/10.1139/m95-168.

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The pink-pigmented facultatively methylotrophic bacterium Methylobacterium rhodesianum MB 126 is able to grow on methanol as the sole source of carbon and energy. Under certain conditions, e.g., limitation of ammonium, phosphate, or oxygen, carbon from methanol is channeled into poly(3-hydroxybutyric acid) (PHB) whereas other polymers or metabolites are hardly overproduced. A mutant of this strain, which we isolated after chemical mutagenesis, is impaired in its ability to synthesize PHB. Under the conditions mentioned above, the mutant still accumulated PHB, but in the absence of ammonium it

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23

Jewell, Talia, Sherry L. Huston, and Douglas C. Nelson. "Methylotrophy in Freshwater Beggiatoa alba Strains." Applied and Environmental Microbiology 74, no. 17 (2008): 5575–78. http://dx.doi.org/10.1128/aem.00379-08.

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ABSTRACT Two freshwater strains of the gammaproteobacterium Beggiatoa alba, B18LD and OH75-2a, are able to use methanol as a sole carbon and energy source under microoxic conditions. Genes encoding a methanol dehydrogenase large-subunit homolog and four enzymes of the tetrahydromethanopterin-dependent C1 oxidation pathway were identified in B18LD. No evidence of methanotrophy was detected.

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24

Ochsner, Andrea M., Frank Sonntag, Markus Buchhaupt, Jens Schrader, and Julia A. Vorholt. "Methylobacterium extorquens: methylotrophy and biotechnological applications." Applied Microbiology and Biotechnology 99, no. 2 (2014): 517–34. http://dx.doi.org/10.1007/s00253-014-6240-3.

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25

Hennig, Guido, Carsten Haupka, Luciana F. Brito, et al. "Methanol-Essential Growth of Corynebacterium glutamicum: Adaptive Laboratory Evolution Overcomes Limitation due to Methanethiol Assimilation Pathway." International Journal of Molecular Sciences 21, no. 10 (2020): 3617. http://dx.doi.org/10.3390/ijms21103617.

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Methanol is a sustainable substrate for biotechnology. In addition to natural methylotrophs, metabolic engineering has gained attention for transfer of methylotrophy. Here, we engineered Corynebacterium glutamicum for methanol-dependent growth with a sugar co-substrate. Heterologous expression of genes for methanol dehydrogenase from Bacillus methanolicus and of ribulose monophosphate pathway genes for hexulose phosphate synthase and isomerase from Bacillus subtilis enabled methanol-dependent growth of mutants carrying one of two independent metabolic cut-offs, i.e., either lacking ribose-5-ph

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26

Hung, Wei-Lian, William G. Wade, Rich Boden, Donovan P. Kelly, and Ann P. Wood. "Facultative methylotrophs from the human oral cavity and methylotrophy in strains of Gordonia, Leifsonia, and Microbacterium." Archives of Microbiology 193, no. 6 (2011): 407–17. http://dx.doi.org/10.1007/s00203-011-0689-6.

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27

Yomantas, Yurgis A. V., Irina L. Tokmakova, Natalya V. Gorshkova, et al. "Aromatic Amino Acid Auxotrophs Constructed by Recombinant Marker Exchange in Methylophilus methylotrophus AS1 Cells Expressing the aroP-Encoded Transporter of Escherichia coli." Applied and Environmental Microbiology 76, no. 1 (2009): 75–83. http://dx.doi.org/10.1128/aem.02217-09.

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ABSTRACT The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxotrophic for aromatic amino acids. The procedure begins with the Mu-driven integration of the Escherichia coli gene aroP, which encodes the common aromatic amino acid transporter, into the genome of M. methylotrophus. The resulting recombinant strain, with improved permeability to certain ami

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28

McTaggart, Tami, David Beck, Usanisa Setboonsarng, et al. "Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria." Microorganisms 3, no. 1 (2015): 94–112. http://dx.doi.org/10.3390/microorganisms3010094.

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29

Takeya, Tomoyuki, Miyabi Yamakita, Daisuke Hayashi, Kento Fujisawa, Yasuyoshi Sakai, and Hiroya Yurimoto. "Methanol production by reversed methylotrophy constructed in Escherichia coli." Bioscience, Biotechnology, and Biochemistry 84, no. 5 (2020): 1062–68. http://dx.doi.org/10.1080/09168451.2020.1715202.

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30

Wang, Yu, Liwen Fan, Philibert Tuyishime, Ping Zheng, and Jibin Sun. "Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing." Trends in Biotechnology 38, no. 6 (2020): 650–66. http://dx.doi.org/10.1016/j.tibtech.2019.12.013.

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31

Lv, Haoxin, and Akio Tani. "Genomic characterization of methylotrophy of Oharaeibacter diazotrophicus strain SM30T." Journal of Bioscience and Bioengineering 126, no. 6 (2018): 667–75. http://dx.doi.org/10.1016/j.jbiosc.2018.05.023.

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32

Agafonova, N. V., N. V. Doronina, E. N. Kaparullina, et al. "A novel Delftia plant symbiont capable of autotrophic methylotrophy." Microbiology 86, no. 1 (2017): 96–105. http://dx.doi.org/10.1134/s0026261717010039.

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33

Martinez-Gomez, N. Cecilia, Nathan Good, Alexa Zytnick, and Morgan Su. "Abstract 2174: The surprising connection between lanthanides and methylotrophy." Journal of Biological Chemistry 299, no. 3 (2023): S333. http://dx.doi.org/10.1016/j.jbc.2023.103642.

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34

Karaseva, Tatiana, Dmitry Fedorov, Sophia Baklagina, et al. "Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from Methylorubrum extorquens AM1 for the Development of Bioelectrocatalytical Systems." International Journal of Molecular Sciences 23, no. 18 (2022): 10337. http://dx.doi.org/10.3390/ijms231810337.

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(Ca2+)-dependent pyrroloquinolinequinone (PQQ)-dependent methanol dehydrogenase (MDH) (EC: 1.1.2.7) is one of the key enzymes of primary C1-compound metabolism in methylotrophy. PQQ-MDH is a promising catalyst for electrochemical biosensors and biofuel cells. However, the large-scale use of PQQ-MDH in bioelectrocatalysis is not possible due to the low yield of the native enzyme. Homologously overexpressed MDH was obtained from methylotrophic bacterium Methylorubrum extorquens AM1 by cloning the gene of only one subunit, mxaF. The His-tagged enzyme was easily purified by immobilized metal ion a

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35

Chistoserdova, Ludmila. "Methylotrophy in a Lake: from Metagenomics to Single-Organism Physiology." Applied and Environmental Microbiology 77, no. 14 (2011): 4705–11. http://dx.doi.org/10.1128/aem.00314-11.

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ABSTRACTThis review provides a brief summary of ongoing studies in Lake Washington (Seattle, WA) directed at an understanding of the content and activities of microbial communities involved in methylotrophy. One of the findings from culture-independent approaches, including functional metagenomics, is the prominent presence ofMethyloteneraspecies in the site and their inferred activity in C1metabolism, highlighting the local environmental importance of this group. Comparative analyses of individual genomes ofMethylophilaceaefrom Lake Washington provide insights into their genomic divergence an

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36

Woolston, Benjamin M., Timothy Roth, Ishwar Kohale, David R. Liu, and Gregory Stephanopoulos. "Development of a formaldehyde biosensor with application to synthetic methylotrophy." Biotechnology and Bioengineering 115, no. 1 (2017): 206–15. http://dx.doi.org/10.1002/bit.26455.

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37

Krüsemann, Jan L., Vittorio Rainaldi, Charles AR Cotton, Nico J. Claassens, and Steffen N. Lindner. "The cofactor challenge in synthetic methylotrophy: bioengineering and industrial applications." Current Opinion in Biotechnology 82 (August 2023): 102953. http://dx.doi.org/10.1016/j.copbio.2023.102953.

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38

Kalyuzhnaya, Marina G., Krassimira R. Hristova, Mary E. Lidstrom, and Ludmila Chistoserdova. "Characterization of a Novel Methanol Dehydrogenase in Representatives of Burkholderiales: Implications for Environmental Detection of Methylotrophy and Evidence for Convergent Evolution." Journal of Bacteriology 190, no. 11 (2008): 3817–23. http://dx.doi.org/10.1128/jb.00180-08.

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ABSTRACT Some members of Burkholderiales are able to grow on methanol but lack the genes (mxaFI) responsible for the well-characterized two-subunit pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenase that is widespread in methylotrophic Proteobacteria. Here, we characterized novel, mono-subunit enzymes responsible for methanol oxidation in four strains, Methyloversatilis universalis FAM5, Methylibium petroleiphilum PM1, and unclassified Burkholderiales strains RZ18-153 and FAM1. The enzyme from M. universalis FAM5 was partially purified and subjected to matrix-assisted laser

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39

Chistoserdova, Ludmila, Sung-Wei Chen, Alla Lapidus, and Mary E. Lidstrom. "Methylotrophy in Methylobacterium extorquens AM1 from a Genomic Point of View." Journal of Bacteriology 185, no. 10 (2003): 2980–87. http://dx.doi.org/10.1128/jb.185.10.2980-2987.2003.

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40

Ochsner, Andrea M., Matthias Christen, Lucas Hemmerle, Rémi Peyraud, Beat Christen, and Julia A. Vorholt. "Transposon Sequencing Uncovers an Essential Regulatory Function of Phosphoribulokinase for Methylotrophy." Current Biology 27, no. 17 (2017): 2579–88. http://dx.doi.org/10.1016/j.cub.2017.07.025.

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41

Antoniewicz, Maciek R. "Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production." Current Opinion in Biotechnology 59 (October 2019): 165–74. http://dx.doi.org/10.1016/j.copbio.2019.07.001.

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42

Dubey, Abhishek Anil, and Vikas Jain. "Mycofactocin is essential for the establishment of methylotrophy in Mycobacterium smegmatis." Biochemical and Biophysical Research Communications 516, no. 4 (2019): 1073–77. http://dx.doi.org/10.1016/j.bbrc.2019.07.008.

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43

Krause, Sascha M. B., Timothy Johnson, Yasodara Samadhi Karunaratne, et al. "Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions." Proceedings of the National Academy of Sciences 114, no. 2 (2016): 358–63. http://dx.doi.org/10.1073/pnas.1619871114.

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The utilization of methane, a potent greenhouse gas, is an important component of local and global carbon cycles that is characterized by tight linkages between methane-utilizing (methanotrophic) and nonmethanotrophic bacteria. It has been suggested that the methanotroph sustains these nonmethanotrophs by cross-feeding, because subsequent products of the methane oxidation pathway, such as methanol, represent alternative carbon sources. We established cocultures in a microcosm model system to determine the mechanism and substrate that underlay the observed cross-feeding in the environment. Lant

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44

Kane, Staci R., Anu Y. Chakicherla, Patrick S. G. Chain, et al. "Whole-Genome Analysis of the Methyl tert-Butyl Ether-Degrading Beta-Proteobacterium Methylibium petroleiphilum PM1." Journal of Bacteriology 189, no. 5 (2006): 1931–45. http://dx.doi.org/10.1128/jb.01259-06.

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ABSTRACT Methylibium petroleiphilum PM1 is a methylotroph distinguished by its ability to completely metabolize the fuel oxygenate methyl tert-butyl ether (MTBE). Strain PM1 also degrades aromatic (benzene, toluene, and xylene) and straight-chain (C5 to C12) hydrocarbons present in petroleum products. Whole-genome analysis of PM1 revealed an ∼4-Mb circular chromosome and an ∼600-kb megaplasmid, containing 3,831 and 646 genes, respectively. Aromatic hydrocarbon and alkane degradation, metal resistance, and methylotrophy are encoded on the chromosome. The megaplasmid contains an unusual t-RNA is

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Strovas, Tim J., Linda M. Sauter, Xiaofeng Guo, and Mary E. Lidstrom. "Cell-to-Cell Heterogeneity in Growth Rate and Gene Expression in Methylobacterium extorquens AM1." Journal of Bacteriology 189, no. 19 (2007): 7127–33. http://dx.doi.org/10.1128/jb.00746-07.

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ABSTRACT Cell-to-cell heterogeneity in gene expression and growth parameters was assessed in the facultative methylotroph Methylobacterium extorquens AM1. A transcriptional fusion between a well-characterized methylotrophy promoter (PmxaF ) and gfpuv (encoding a variant of green fluorescent protein [GFPuv]) was used to assess single-cell gene expression. Using a flowthrough culture system and laser scanning microscopy, data on fluorescence and cell size were obtained over time through several growth cycles for cells grown on succinate or methanol. Cells were grown continuously with no discerni

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Butterfield, Cristina N., Zhou Li, Peter F. Andeer, et al. "Proteogenomic analyses indicate bacterial methylotrophy and archaeal heterotrophy are prevalent below the grass root zone." PeerJ 4 (November 8, 2016): e2687. http://dx.doi.org/10.7717/peerj.2687.

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Annually, half of all plant-derived carbon is added to soil where it is microbially respired to CO2. However, understanding of the microbiology of this process is limited because most culture-independent methods cannot link metabolic processes to the organisms present, and this link to causative agents is necessary to predict the results of perturbations on the system. We collected soil samples at two sub-root depths (10–20 cm and 30–40 cm) before and after a rainfall-driven nutrient perturbation event in a Northern California grassland that experiences a Mediterranean climate. From ten sample

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Fassel, T. A., M. J. Schaller, M. E. Lidstrom, and C. C. Remsen. "Comparative surface and ultrastructural views of methylotrophic bacteria by TEM, STEM, SE, and freeze-etch electron microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 792–93. http://dx.doi.org/10.1017/s0424820100128274.

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Methylotrophic bacteria play an Important role in the environment in the oxidation of methane and methanol. Extensive intracytoplasmic membranes (ICM) have been associated with the oxidation processes in methylotrophs and chemolithotrophic bacteria. Classification on the basis of ICM arrangement distinguishes 2 types of methylotrophs. Bundles or vesicular stacks of ICM located away from the cytoplasmic membrane and extending into the cytoplasm are present in Type I methylotrophs. In Type II methylotrophs, the ICM form pairs of peripheral membranes located parallel to the cytoplasmic membrane.

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Beck, David A. C., Tami L. McTaggart, Usanisa Setboonsarng, et al. "Multiphyletic origins of methylotrophy inAlphaproteobacteria, exemplified by comparative genomics of Lake Washington isolates." Environmental Microbiology 17, no. 3 (2015): 547–54. http://dx.doi.org/10.1111/1462-2920.12736.

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Nercessian, Olivier, Emma Noyes, Marina G. Kalyuzhnaya, Mary E. Lidstrom, and Ludmila Chistoserdova. "Bacterial Populations Active in Metabolism of C1 Compounds in the Sediment of Lake Washington, a Freshwater Lake." Applied and Environmental Microbiology 71, no. 11 (2005): 6885–99. http://dx.doi.org/10.1128/aem.71.11.6885-6899.2005.

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ABSTRACT Active members of the bacterial community in the sediment of Lake Washington, with special emphasis on C1 utilizers, were identified by employing two complementary culture-independent approaches: reverse transcription of environmental mRNA and 16S rRNA combined with PCR (RT-PCR) and stable-isotope probing (SIP) of DNA with the 13C-labeled C1 substrates methanol, methylamine, formaldehyde, and formate. Analysis of RT-PCR-amplified fragments of 16S rRNA-encoding genes revealed that gammaproteobacterial methanotrophs belonging to Methylobacter and Methylomonas dominate the active methylo

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Yurimoto, Hiroya, Kosuke Shiraishi, and Yasuyoshi Sakai. "Physiology of Methylotrophs Living in the Phyllosphere." Microorganisms 9, no. 4 (2021): 809. http://dx.doi.org/10.3390/microorganisms9040809.

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Methanol is abundant in the phyllosphere, the surface of the above-ground parts of plants, and its concentration oscillates diurnally. The phyllosphere is one of the major habitats for a group of microorganisms, the so-called methylotrophs, that utilize one-carbon (C1) compounds, such as methanol and methane, as their sole source of carbon and energy. Among phyllospheric microorganisms, methanol-utilizing methylotrophic bacteria, known as pink-pigmented facultative methylotrophs (PPFMs), are the dominant colonizers of the phyllosphere, and some of them have recently been shown to have the abil

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