Relevant bibliographies by topics / Metodo Delle Celle

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Metodo Delle Celle'

Author: Grafiati
Published: 11 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "Metodo Delle Celle"

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Bieńko, Karolina, Monika Leszcz, Justyna Białek, Marta Więckowska-Deroń, Dominika Ćwik-Błotnicka, and Joanna Borczyk. "2D and 3D culture of stem cells." Journal of Education, Health and Sport 16, no. 1 (2023): 62–67. http://dx.doi.org/10.12775/jehs.2023.16.01.007.

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Wstęp i cel: Komórki macierzyste są szeroko stosowane w wielu naukach, szczególnie w chorobach. Dzięki swoim właściwościom proliferacyjnym i zdolności do tworzenia się w innych elementach organizmu zastosowanie w mechanizmie regeneracyjnym, przy zachowaniu charakterystycznych parametrów oparzeń, odbudowie układu krwiotwórczego. później je pozyskać z wielu dzieci m.in. składników tłuszczowej, szpiku kostnego, krwi pępowinowej czy mleka kobiecego. 
 Obecny stan wiedzy: Dlatego ważne jest, aby wykorzystać metodę hodowli, dzięki której w krótkim czasie możemy uzyskać wyniki badań, które pozwo
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Tordera, Daniel, and Henk J. Bolink. "Perovskite solar cells." Metode Science Studies Journal 15, no. 2 (2025): e28390. https://doi.org/10.7203/metode.15.28390.

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At present, there is an urgent need to reduce greenhouse gas emissions to mitigate the climate change that threatens humanity and our planet’s ecosystems. A way to achieve this is by increasing renewable energy production, where solar photovoltaic plays a key role. However, the current commercial crystalline silicon photovoltaic technology might not be enough to achieve the required targets. In this work, we describe the latest advances of an emerging photovoltaic technology known as perovskites. In just ten years of development perovskite solar cells have matched the performance of current co
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RemyaV, RemyaV, Naveen Kumar, and Kutty M. V. H. Kutty M.V.H. "A Method for Cell Culture and RNA Extraction of Rabbit Bone Marrow Derived Mesenchymal Stem Cells." International Journal of Scientific Research 3, no. 7 (2012): 31–33. http://dx.doi.org/10.15373/22778179/july2014/11.

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Song, Hyo-won. "English Learners’ Perceptions of the Verb-Centered Learning Method based on Dik’s Functional Grammar." Convergence English Language & Literature Association 9, no. 3 (2024): 197–235. https://doi.org/10.55986/cell.2024.9.3.197.

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Zhen-Ning Wu, Zhen-Ning Wu, Hao-Sen Huang Zhen-Ning Wu, Jun-Jun Jiang Hao-Sen Huang, Zhong-Zhe Xiao Jun-Jun Jiang, and Min Huang Zhong-Zhe Xiao. "Intelligent Tumor Cell Detection Method Based on Circulating Tumor Cell (CTC) Technology." 電腦學刊 34, no. 1 (2023): 117–30. http://dx.doi.org/10.53106/199115992023023401009.

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<p>Cancer has become one of the greatest threats for human life. Doctors can get original images of the sick organs with the assistance of medical technology. However, manual interpretation of the original images is time consuming and labor consuming. Nowadays, the intelligent detection of tumor cell images is commonly adopted in cancer diagnosis. In this paper, we propose Imporved Selective Search (ISS) algorithm and CTCNet based on Circulating Tumor Cell (CTC) technology to improve the cancer images’ detection efficiency. CTCs can be collected by a sampling needle with EpCAM
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B Tushabe, Florence, Samuel Mwesige, Vicent Kasule, et al. "An Image-Based Sickle Cell Detection Method." Engineering and Applied Sciences Journal 2, no. 1 (2025): 01–04. https://doi.org/10.64030/3067-8005.02.01.05.

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This article presents a method of sickle cell detection from microscopic images. We extract five attribute values from the connected components of an image, and train machine learning classifiers to recognize the sickle cells. Four classifiers were experimented with and the vest one was the K-Nearest neighbor classifier with 97.3% accuracy. The other classifiers are the Neutral network, Decision tree and Naïve Bayesian classifiers which resulted in accuracy rates of between 89-96.3%. This method is applicable for use in low cost computers since it is computationally cheap. The findings of this
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Bolzhelarskyi, Yaroslav, Stepan Dovhaniuk, Oleksandr Urshuliak, and Paweł Daszkiewicz. "Traction tests of the special self-propelled rolling stock: aims, methods, experience and prospects." Rail Vehicles, no. 4 (November 2, 2020): 1–11. http://dx.doi.org/10.53502/rail-138557.

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W artykule podane zostały metody i wyniki eksperymentalnego określania dwóch najważniejszych parametrów do obliczeń trakcyjnych samobieżnego pojazdu typu DGK (spalinowy pojazd inspekcyjny). Pierwszym parametrem jest przyśpieszenie, tj. przyspieszenie liczone z działania jednostki siły na jednostkę masy pociągu. Drugim są opory ruchu. Wynik może być wykorzystany do określenia tych parametrów dla różnego typu OTM (on Track Machines).
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Wang Yidong, 王宜东, 杜永兆 Du Yongzhao, 黎玲 Li Ling, 傅玉青 Fu Yuqing та 刁勇 Diao Yong. "基于细胞核引导的明场显微图像细胞分割方法". Laser & Optoelectronics Progress 60, № 14 (2023): 1410015. http://dx.doi.org/10.3788/lop222437.

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Гудилин, Е. А., А. А. Семенова, О. Е. Ерёмина та ін. "Перспективные методы неинвазивной медицинской диагностики с использованием наноматериалов: спектроскопия гигантского комбинационного рассеяния в исследовании клеток, клеточных органелл, маркеров нейромедиаторного обмена". НАНОМЕДИЦИНА, № 6 (30 грудня 2018): 62–73. http://dx.doi.org/10.24075/vrgmu.2018.077.

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Использование достижений наномедицины и материаловедения в диагностике заболеваний является перспективным направлением научных исследований. Спектроскопия гигантского комбинационного рассеяния (ГКР) — инновационный метод анализа, связанный с применением наноматериалов на основе благородных металлов для неинвазивного исследования клеток, клеточных органелл, белковых молекул. В работе обобщены литературные данные по методам ранней клинической диагностики ряда нейродегенеративных и нейроэндокринных заболеваний. Обсуждены особенности, достоинства и ограничения различных методов диагностики по низк
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Ke Baosheng, 柯宝生, 李颖 Li Ying, 任振波 Ren Zhenbo, 邸江磊 Di Jianglei та 赵建林 Zhao Jianlin. "基于深度学习的活体细胞有丝分裂检测方法". Acta Optica Sinica 41, № 15 (2021): 1511001. http://dx.doi.org/10.3788/aos202141.1511001.

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Dissertations / Theses on the topic "Metodo Delle Celle"

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TIMO', GIANLUCA. "Un nuovo metodo per realizzare celle a multigiunzione ad alta efficienza e basso costo combinando gli elementi III-IV-V della tavola periodica." Doctoral thesis, Università degli studi di Pavia, 2021. http://hdl.handle.net/11571/1437335.

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This thesis assesses the potential of a new technological path for combining III-V and IV elements, in order to exploit the band gap engineering possibilities which this integration offers and set up the basis for realizing, at low cost, high efficiency - monolithically thin - InGaP/InGaAs/SiGeSn/Ge solar cells. A suitable model for evaluating the performance of the MJ solar cell is built up, as well as the growth of the SiGeSn alloy and its integration in the III-V structure, by utilizing a high throughput MOVPE equipment. TMM has then been applied in the modelling of a MJ cell structure desi
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Pani, Martino. "Sviluppo, valutazione ed applicazione di metodi numerici alternativi al medodo degli elementi finiti in problemi di biomeccanica ortopedica." Doctoral thesis, Università degli studi di Trieste, 2010. http://hdl.handle.net/10077/3674.

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2008/2009<br>La predizione del rischio di frattura costituisce un elemento di estremo interesse in ambito clinico quale supporto dei processi diagnostici, di prognosi e di cura; attualmente la valutazione è basata su inferenze statistiche rispetto al contenuto minerale del tessuto osseo in siti specifici a mezzo di speciali immagini radiografiche, realizzandosi valutazioni caratterizzate da un consistente margine di incertezza. Un significativo miglioramento è ragionevole pensare possa derivare dall'uso di modelli numerici tridimensionali subject specific che, fondandosi su immagini diagnostic
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Kanani, Chirantan. "Cell Printing: A novel method to seed cells onto biological scaffolds." Digital WPI, 2012. https://digitalcommons.wpi.edu/etd-theses/332.

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Bioprinting, defined as depositing cells, extracellular matrices and other biologically relevant materials in user-defined patterns to build tissue constructs de novo or to build upon pre-fabricated scaffolds, is among one of the most promising techniques in tissue engineering. Among the various technologies used for Bioprinting, pressure driven systems are most conducive to preserving cell viability. Herein, we explore the abilities of a novel bioprinter - Digilab, Inc.'s prototype cell printer. The prototype cell printer (Digilab Inc., Holliston, MA) is an automated liquid handling device ca
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Genshaft, Alexander S. "Methods to interrogate cells and their interactions with single-cell resolution." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/127892.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, May, 2020<br>Cataloged from the PDF of thesis.<br>Includes bibliographical references.<br>Only recently have molecular methods achieved high-quality and unbiased representations of diverse intracellular molecules at the single-cell level. With this technological advancement, researchers have begun deconvolving population-level measurements to understand whether prior observations were homo- or heterogeneous across the sample. In order to make multi-omics workflows compatible with low input samples comprising a few
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Timmins, Nicholas E. "Extending the third dimension : novel methods and applications for 3D multicellular spheroids /." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18289.pdf.

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Hillis, Yingli, and Yingli Hillis. "Validation of a Semi-Automatic Cell Segmentation Method to the Manual Cell Counting Method on Identifying Proliferating Cells in 3-D Confocal Microscope Images." Thesis, The University of Arizona, 2017. http://hdl.handle.net/10150/626739.

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Sphere-forming assay is an in-vitro technique to assess the self-renewal and differentiation potential of a homogenous or heterogenous population of cells. This technique is commonly used in the stem cell and cancer biology fields to assess the ability of a cell that is capable of self-proliferation and differentiation. (Schmitt, 2011, Lombaert et al., 2008) To detect proliferative growth, Ki-67, a marker of proliferation, is used in immunofluorescence staining of sphere-forming cells. The current gold standard methodology to quantify cell proliferation is to manually count the cells on images
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Lähdesmäki, Ilkka Johannes. "Flow injection methods for drug-receptor interaction studies, based on probing cell metabolism /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/8590.

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Postigo, Ivan. "Fingerprinting methods for positioning: A study on the adaptive enhanced cell identity method." Thesis, Linköpings universitet, Kommunikations- och transportsystem, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-153314.

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Fingerprinting methods for positioning is an area of great interest, this thesis presents a study on the Adaptive Enhanced Cell Identity (AECID) fingerprinting method for positioning. By creating a map of the radio characteristics in a geographical region, the AECID method is able to locate a UE by gathering information of the radio conditions of its current location. By performing positioning in this manner, there is no need for additional signaling, which is a better usage of the radio resources. This thesis presents a new approach for the creation of fingerprints together with alternative m
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Phipps, Laura Ellen. "Targeting cell adhesion as a method of sensitising metastatic tumour cells to TRAIL-induced apoptosis." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:93198943-a7fa-4b30-aaed-c92d7e0a4ba6.

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Due to its selectivity in killing cancer cells, Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) has provided a potential new agent for cancer treatment. However, despite promising pre-clinical results, it appears that TRAIL therapies will be most effective when used in combination with a sensitizing agent. In light of previous evidence suggesting that cell adhesion could influence sensitivity to Tumour necrosis factor family ligands, this thesis presents a study of the effects of disrupting matrix adhesion on the sensitivity of human MDA-MB-231 breast and 1205Lu melanoma cell
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Liu, Hong. "Optimization for Fuel Cells/Fuel Cell Stacks Using Combined Methods---CFD Modeling Analysis, and Experiments." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/297027.

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Fuel cells are one of most environmental friendly energy sources; they have many advantages and may be used in many applications from portable electronic devices to automotive components. Proton exchange membrane (PEM) fuel cells are one of most reliable fuel cells and have advantage such as rapid-startup and ease of operation. This dissertation focuses on PEM fuel cell stack optimization based on operation experimental research and numerical modeling study. This dissertation presents three major research activities and the obtained results by the Ph.D candidate. A novel stack architecture des
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Books on the topic "Metodo Delle Celle"

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Golka, Marian. Nauczanie sztuki: Cele, formy, metody. Państwowa Wyższa Szkoła Sztuk Plastycznych, 1991.

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Vithoulkas, George. The Celle seminars. Ulrich Burgdorf, Homoeopathic Pub. House, 1992.

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Tabaczyński, Stanisław. Archeologia średniowieczna: Problemy, źródła, metody, cele badawcze. Zakład Narodowy im. Ossolińskich, 1987.

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Dr, Sullivan Stephen, Eggan Kevin, and Cowan Chad, eds. Human embryonic stem cells: The practical handbook. John Wiley & Sons, 2007.

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V, Polevoĭ V., Maksimov G. B, Sini͡u︡tina N. F та Leningradskiĭ gosudarstvennyĭ universitet imeni A.A. Zhdanova., ред. Metody izuchenii͡a︡ membran rastitelʹnykh kletok: Uchebnoe posobie. Izd-vo Leningradskogo universiteta, 1986.

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L, Sorensen Mikkel, ed. Stem cell applications in diseases. Nova Biomedical Books, 2008.

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Masters, John R. Human Adult Stem Cells. Springer Netherlands, 2009.

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Bain, Barbara J. A Beginner's Guide to Blood Cells. John Wiley & Sons, Ltd., 2007.

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Antonella, Macagnano, ed. Advanced topics in cell model systems. Nova Science Publishers, 2009.

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Basin, A. S. Poluchenie kremnievykh plastin dli͡a solnechnoĭ ėnergetiki: Metody i tekhnologii. In-t teplofiziki SO RAN, 2000.

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Book chapters on the topic "Metodo Delle Celle"

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Vermeersch, Lieselotte, Abbas Jariani, Jana Helsen, Benjamin M. Heineike, and Kevin J. Verstrepen. "Single-Cell RNA Sequencing in Yeast Using the 10× Genomics Chromium Device." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2257-5_1.

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AbstractSingle-cell RNA sequencing (scRNA-seq) is emerging as an essential technique for studying the physiology of individual cells in populations. Although well-established and optimized for mammalian cells, research of microorganisms has been faced with major technical challenges for using scRNA-seq, because of their rigid cell wall, smaller cell size and overall lower total RNA content per cell. Here, we describe an easy-to-implement adaptation of the protocol for the yeast Saccharomyces cerevisiae using the 10× Genomics platform, originally optimized for mammalian cells. Introducing Zymol
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Logette, Emmanuelle, and Rajnish Ranjan. "Ready-to-Record Cells for Kinetic Screening of VGICs." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3818-7_5.

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AbstractVoltage-gated ion channels (VGICs) are integral membrane proteins crucial for transmitting electrical signals in excitable cells. Understanding the kinetics of these ion channels requires conducting patch-clamp experiments using genetically modified cell lines that express a single type of ion channel gene. However, this process relies on the continuous maintenance of cell lines to ensure an adequate supply of sample cells for patch-clamp experiments. Advancements in automated patch-clamp methods have enabled researchers to significantly increase the number of patch-clamped cells per e
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Lehmann, Alexander A., Diana R. Roen, Zoltán Megyesi, and Paul V. Lehmann. "Reagent Tracker™ Platform Verifies and Provides Audit Trails for the Error-Free Implementation of T-Cell ImmunoSpot® Assays." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3690-9_7.

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AbstractELISPOT and FluoroSpot assays, collectively called ImmunoSpot assays, permit to reliable detection of rare antigen-specific T cells in freshly isolated cell material, such as peripheral blood mononuclear cells (PBMC). Establishing their frequency within all PBMC permits to assess the magnitude of antigen-specific T-cell immunity; the simultaneous measurement of their cytokine signatures reveals these T-cells’ lineage and effector functions, that is, the quality of T-cell-mediated immunity. Because of their unparalleled sensitivity, ease of implementation, robustness, and frugality in P
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Elvevold, Kjetil, Ingelin Kyrrestad, and Bård Smedsrød. "Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_27.

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AbstractDevelopment of the new generation of drugs (e.g., oligo- and polynucleotides administered intravascularly either as free compounds or as nano-formulations) frequently encounters major challenges such as lack of control of targeting and/or delivery. Uncontrolled or unwanted clearance by the liver is a well-known and particularly important hurdle in this respect. Hence, reliable techniques are needed to identify the type(s) of liver cells, receptors, and metabolic mechanisms that are responsible for unwanted clearance of these compounds.We describe here a method for the isolation and cul
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Karulin, Alexey Y., Melinda Katona, Zoltán Megyesi, Greg A. Kirchenbaum, and Paul V. Lehmann. "Artificial Intelligence-Based Counting Algorithm Enables Accurate and Detailed Analysis of the Broad Spectrum of Spot Morphologies Observed in Antigen-Specific B-Cell ELISPOT and FluoroSpot Assays." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3690-9_5.

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AbstractAntigen-specific B-cell ELISPOT and multicolor FluoroSpot assays, in which the membrane-bound antigen itself serves as the capture reagent for the antibodies that B cells secrete, inherently result in a broad range of spot sizes and intensities. The diversity of secretory footprint morphologies reflects the polyclonal nature of the antigen-specific B cell repertoire, with individual antibody-secreting B cells in the test sample differing in their affinity for the antigen, fine epitope specificity, and activation/secretion kinetics. To account for these heterogeneous spot morphologies,
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Pirincci Ercan, Deniz, and Frank Uhlmann. "Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1538-6_19.

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AbstractThe cell cycle is an ordered series of events by which cells grow and divide to give rise to two daughter cells. In eukaryotes, cyclin–cyclin-dependent kinase (cyclin–Cdk) complexes act as master regulators of the cell division cycle by phosphorylating numerous substrates. Their activity and expression profiles are regulated in time. The budding yeast S. cerevisiae was one of the pioneering model organisms to study the cell cycle. Its genetic amenability continues to make it a favorite model to decipher the principles of how changes in cyclin-Cdk activity translate into the intricate s
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Mukherjee, Nabanita, Karoline A. Lambert, David A. Norris, and Yiqun G. Shellman. "Enrichment of Melanoma Stem-Like Cells via Sphere Assays." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1205-7_14.

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AbstractSphere assays are widely used in vitro techniques to enrich and evaluate the stem-like cell behavior of both normal and cancer cells. Utilizing three-dimensional in vitro sphere culture conditions provide a better representation of tumor growth in vivo than the more common monolayer cultures. We describe how to perform primary and secondary sphere assays, used for the enrichment and self-renewability studies of melanoma/melanocyte stem-like cells. Spheres are generated by growing melanoma cells at low density in nonadherent conditions with stem cell media. We provide protocols for prep
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Stacey, Glyn N., and Otto-Wilhelm Merten. "Host Cells and Cell Banking." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-095-9_3.

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Krüger, Lara K., Joseph L. Watson, and Emmanuel Derivery. "Synthetic Engineering of Cortical Polarity During Mitosis Using Designed Proteins." In Methods in Molecular Biology. Springer US, 2024. https://doi.org/10.1007/978-1-0716-4224-5_17.

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AbstractDuring asymmetric cell division, cortical polarity cues drive the polarization of the microtubule cytoskeleton to ensure the generation of two daughter cells with different fates. While this a critical process for development and tissue homeostasis, the underlying molecular mechanisms orchestrating those processes are not completely understood, especially in mammals. Here, we present an assay that allows the study of the molecular mechanisms driving mammalian asymmetric cell division in a high-throughput manner by capitalizing on protein design to engineer cortical polarity of virtuall
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Buzgariu, Wanda, Jean-Pierre Aubry-Lachainaye, and Brigitte Galliot. "Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2172-1_20.

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AbstractThe freshwater Hydra polyp is a versatile model to study whole-body regeneration from a developmental as well as a cellular point of view. The outstanding regenerative capacities of Hydra are based on its three populations of adult stem cells located in the central body column of the animal. There, these three populations, gastrodermal epithelial, epidermal epithelial, and interstitial, continuously cycle in homeostatic conditions, and their activity is locally regulated after mid-gastric bisection. Moreover, they present an unusual cycling behavior with a short G1 phase and a pausing
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Conference papers on the topic "Metodo Delle Celle"

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Inose, Tomoko. "Plasmonic nanowire based intracellular material delivery." In JSAP-Optica Joint Symposia. Optica Publishing Group, 2024. https://doi.org/10.1364/jsapo.2024.16p_b4_1.

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The technology for introducing biomolecules such as proteins and DNA into cells is widely used as a method to artificially control cell functions, ranging from basic biology to the pharmaceutical field. Methods employing liposomes, viral vectors, and the electroporation have been currently widely used, although these methods show low introduction efficiency or cell toxicity to some cell types. Another method for introducing biomolecules into cells is microinjection.1) This method physically introduces micro/nano needles directly into the cells, ensuring that biomolecules are reliably delivered
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Wu, Weishu, Yu Zhang, Xiaotian Tan, Sunitha Nagrath, and Xudong Fan. "Antigen-independent single-cell detection of circulating tumor cells using biolaser." In CLEO: Applications and Technology. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_at.2024.atu3b.6.

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We developed a deep learning-powered biolaser platform that can differentiate cell types on a single-cell basis without immuno-staining. This method can achieve around 95% accuracy differentiating circulating tumor cells from white blood cells.
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Pace, Joshua, Nicole Rueb, Gauri Malankar, et al. "Development and Evaluation of Fluorescence Contrast Agents for in Vivo Counting of Circulating Cancer Cells." In Optical Molecular Probes, Imaging and Drug Delivery. Optica Publishing Group, 2025. https://doi.org/10.1364/omp.2025.oth1d.4.

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The goal of this research is to develop a method to label and detect circulating tumor cells in vivo with different types of injectable cell targeted fluorescent molecular contrast agents while limiting immune cell labeling.
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Ringwelski, Beth, Vidura Jayasooriya, and Dharmakeerthi Nawarathna. "Label Free Cell Purification Following Electroporation." In 2020 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/dmd2020-9037.

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Abstract Cell transfection by electroporation is a biological assay that has been utilized to inject exogenous molecules (e.g.: RNA, DNA and protein) into live cells. Recently, electroporation has been utilized in developing cell therapy for cancer (e.g., CAR T-cell). One of the major drawbacks in current electroporation methods is the cell death during the process. These dead cells can be detrimental, if injected back to the patients. Current cell filtering methods are unable purify T-cells following electroporation, this is due to the lack of unique biomarkers that target the apoptosis and n
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Su, Fengmin, Yiming Fan, He Xu, et al. "Ultra-High Speed Vitrification of Prostate Cancer Cells Based on Thin Film Evaporation." In ASME 2019 6th International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/mnhmt2019-3910.

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Abstract Thin film evaporation is an efficient phase change heat transfer style, and could achieve ultra-high cooling rate if it was applied for cells vitrification. In this paper, an experimental study for prostate cancer cells vitrification was done. The cells ultra-high speed freezing method was based on thin film evaporation of liquid nitrogen. In order to examine the feasibility of the new method, the comparison experiments, in which the other two generic approaches of cell cryopreservation were used, were done. The methods were respectively the equilibrium freezing method and the open pu
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Cobb, Jessica A., Alison C. Dunn, Jiwoon Kwan, Malisa Sarntinoranont, Gregory Sawyer, and Roger Tran-Son-Tay. "A Novel Method for Frictional Testing on Adherent Cells." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176552.

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Understanding the physical interaction between biomaterials and tissues within the body is critical for the development of medical devices. For instance, the coefficient of friction between the two must be low enough to avoid damaging the cells that make up the tissue. Therefore, a method for obtaining friction data on cells is needed. In the present work, human corneal epithelial (HCE-T) cells are cultured within specialized cell holders and friction experiments are performed with a micro-tribometer. Cell damage is assessed via microscopy and a trypan blue viability assay. Preliminary experim
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Lapenko, A. K., E. R. Ol’hovaya, P. S. Kupcova, E. R. Lyapunova, and L. N. Komarova. "METHOD FOR ESTIMATING THE NUMBER OF VIABLE CELLS IN A CELL SPHEROID USING THE MTT ASSAY." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-27.

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One of the methods forstudying the viability of cell spheroids isthe MTT test. However, this test does not permit the determination of the number of viable cells within the spheroid at different stages of cultivation. Instead, it provides an indirect indication of the metabolic activity of cells. In this study, we propose a method for estimating the number of viable cells in a cell spheroid using the MTT test.
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Amontree, Jacob, Kangfu Chen, Jose Varillas, and Z. Hugh Fan. "Capillary Force Driven Single-Cell Spiking Apparatus for Studying Circulating Tumor Cells." In ASME 2018 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/imece2018-87109.

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The characterization of single cells within heterogeneous populations has great impact on both biomedical sciences and cancer research. By investigating cellular compositions on a broad scale, pertinent outliers may be lost in the sample set. Alternatively, an investigation focused on the behavior of specific cells, such as circulating tumor cells (CTCs), will reveal genetic biomarkers or phenotypic characteristics associated with cancer and metastasis. On average, CTC concentration in peripheral blood is extremely low, as few as one to two per billion of healthy blood cells. Consequently, the
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Han, Taehee, Tessa A. Haagenson, Hossein Salehfar, Samir Dahal, and Mike D. Mann. "An Efficient Approximation Method for an Individual Fuel Cell Impedance Characterization." In ASME 2010 8th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2010. http://dx.doi.org/10.1115/fuelcell2010-33345.

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In this study, an efficient method of approximating individual fuel cell impedances in a stack is proposed and experimentally verified. Two different proton exchange membrane (PEM) fuel cell stacks (600 W with 24 cells and 1.2 kW with 47 cells) were used to develop and verify the method. Both PEM fuel cell stacks were operated using room air and pure hydrogen (99.999%). Impedance and current - voltage (I-V) data were collected for stack and individual cell levels under various operating conditions. The experimental result shows that the individual cell impedance is directly proportional to the
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Gupta, Vijay K., and Charles D. Eggleton. "A Numerical Method for Coupling Nano-Scale Molecular Binding With Mesoscale Cellular Deformation." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13296.

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Cell adhesion plays a pivotal role in diverse biological processes, including inflammation and thrombosis. Changes in cell adhesion can be the defining event in a wide range of diseases, including cancer, osteoporosis, atherosclerosis, and arthritis. Cells are exposed constantly to hemodynamic/hydrodynamic forces and the balance between the dispersive hydrodynamic forces and the adhesive forces generated by the interactions of membrane-bound receptors and their ligands determines cell adhesion. The ultimate objective of our work is to develop software that can simulate the adhesion of cells co
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Reports on the topic "Metodo Delle Celle"

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Singh, Anjali. Ultimate Guide to Automated Cell Counter: Plus Purchasing Tips. ConductScience, 2022. http://dx.doi.org/10.55157/cs20220614.

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An automated cell counter is a machine that uses either image analysis or electrical impedance principles to count cells automatically. The electrical impedance principle involves measuring changes in electrical resistance as cells pass through an aperture, while the light-scattering principle observes how cells scatter light when exposed to it. There are four main types of automated cell counting methods: Coulter Counter, Image Analysis Method, Flow Cytometry, and Stereological Cell Counting. Each method has its benefits and limitations, offering faster and more objective cell counting compar
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หังสสูต, ปกรัฐ, та ญาดา ตันสิริ. การวิเคราะห์การยับยั้งการเพิ่มจำนวนของไวรัสเอชไอวีโดยทีเซลล์ : การทดสอบที่ใช้วิเคราะห์ภูมิคุ้มกันที่ได้จากการกระตุ้นด้วยวัคซีนป้องกัน HIV : รายงานการวิจัย. คณะแพทยศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2013. https://doi.org/10.58837/chula.res.2013.14.

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Introduction and Objective: Among HIV infected donors, the natural history of HIV infection is different and HIV load is the one of factors resulting in the different clinical outcome. We have been characterized HIV infected donors into 2 groups: controllers (HIV loads&lt; 2,000 copies/ml) and noncontrollers (HIV loads &gt; 2,000 copies/ml). In our previous study, we found that controllers who naturally control HIV infection have the higher number of HIV-gag p24 specific T cells than noncontrollers significantly. Thus, we hypothesized that these polyfunctional T cells can effectively suppress
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หังสสูต, ปกรัฐ, та ญาดา ตันสิริ. การวิเคราะห์การยับยั้งการเพิ่มจำนวนของไวรัสเอชไอวีโดยทีเซลล์ : การทดสอบที่ใช้วิเคราะห์ภูมิคุ้มกันที่ได้จากการกระตุ้นด้วยวัคซีนป้องกัน HIV : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2014. https://doi.org/10.58837/chula.res.2014.19.

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Introduction and Objective: Among HIV infected donors, the natural history of HIV infection is different and HIV load is the one of factors resulting in the different clinical outcome. We have been characterized HIV infected donors into 2 groups: controllers (HIV loads &lt;2,000 copies/ml) and noncontrollers (HIV loads &gt;2,000 copies/ml).In our previous study, we founded that controllers who naturally control HIV infection have the higher number of HIV-gag p24 specific T cells than noncontrollers significantly. Thus, We hypothesized that these polyfunctional T cells can effectively suppress
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Styles, Richard, Rachel Bain, and Anthony Priestas. Method to evaluate vessel wake forces on wetland scarps. Engineer Research and Development Center (U.S.), 2022. http://dx.doi.org/10.21079/11681/45304.

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This Coastal and Hydraulics engineering technical note (CHETN) presents a methodology to compute normal forces on wetland perimeters with vertically scarped edges. The approach uses an empirical algorithm that predicts the normal force given the offshore vessel wake height, period, and water depth at a given point. Wave impact forces are measured using load cells, which have not been applied previously to marsh settings. Load cell and vessel wake measurements from two field sites are combined to generate an empirical transfer function relating forces to incoming vessel wake characteristics.
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Calomeni, Alyssa, Andrew McQueen, Ciera Kinley-Baird, and Gerard Clyde. Identification and preventative treatment of overwintering cyanobacteria in sediments : a literature review. Engineer Research and Development Center (U.S.), 2022. http://dx.doi.org/10.21079/11681/45063.

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Freshwaters can experience growths of toxin-producing cyanobacteria or harmful algal blooms (HABs). HAB-producing cyanobacteria can develop akinetes, which are thick-enveloped quiescent cells akin to seeds in vascular plants or quiescent colonies that overwinter in sediment. Overwintering cells produce viable “seed beds” for HAB resurgences and preventative treatments may diminish HAB intensity. The purpose of this literature review was to identify (1) environmental factors triggering germination and growth of overwintering cells, (2) sampling, identification, and enumeration methods, and (3)
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กาญจนบุษย์, เถลิงศักดิ์, กุลวรา เมฆสวรรค์, อังคณา ตันติธุวานนท์, วิภาวี กิตติโกวิท, พรทิพย์สวรรค์ นวลทอง та พรเพ็ญ พนมวัลย์. การพัฒนาและการศึกษาความเป็นไปได้ในการนำน้ำตาลเชิงซ้อน-แคลวิตินมาผลิตเป็นน้ำยาฟอกไตทางช่องท้องทดแทนน้ำยาฟอกไตมาตรฐาน. คณะแพทยศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2010. https://doi.org/10.58837/chula.res.2010.17.

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Background. The optimal formula of calvitrin based peritoneal dialysis was composing of calvitrin 6.25 g LL~:: electrolytes such as sodium chloride 540 g, sodium lactate 448 mg, calcium chloride 25.7mg and magnesium chloride 5.08 mg Methods. In vitro studies; the cytotoxicity was investigated. Human peritoneal mesothelial cells (HPMC) were isolated and characterized as described in tetail elsewhere (29). Cells were depleated serum and treated with 15% Cal-PD, 7.5% lcodextrin, 1.5% Dextose and media controls for 36 hrs. Cells morphology changes were examined under microscopy. The results found
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Archived), Richard Eckert (. PR-186-04301-R01 Development of a Bench Test Method for Microbiologically Influenced Corrosion (MIC). Pipeline Research Council International, Inc. (PRCI), 2005. https://doi.org/10.55274/r0000112.

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The objectives of the research were to: (1) Define laboratory test parameters and select equipment that can consistently produce MIC initiation, (2) Establish clear protocols for conducting the testing and performing analysis of the colonized/ corroded surfaces, (3) Communicate these protocols in a straightforward manner, (4) Establish test control standards for biotic and abiotic coupon exposure results under various flow regimes, (5) Investigate precursors to cell attachment on steel surfaces for the test conditions specified, and (6) Correlate lab test coupons with corrosion observed on cou
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Coulson, McGrath, and McCarthy. PR-312-14206-R01 Considerations for Developing a New Electrochemical Cell Portable Analyzer Test Method. Pipeline Research Council International, Inc. (PRCI), 2015. http://dx.doi.org/10.55274/r0010156.

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The basis of electrochemical cell gas sensor technology is presented in the context of electrochemical cell portable analyzers for emissions testing of NOx and CO. This paper presents a modified test method approach and QA/QC criteria that supplement the project goal of assessing existing portable analyzer test methods and protocols for appropriate QA/QC requirements. The goal of a modified method is to minimize testing costs while ensuring data quality. The modified method leverages the inherent linearity of electrochemical cell technology, based on fundamental scientific principles, to emplo
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Semaan, Dima, and Linda Scobie. Feasibility study for in vitro analysis of infectious foodborne HEV. Food Standards Agency, 2022. http://dx.doi.org/10.46756/sci.fsa.wfa626.

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Hepatitis E virus (HEV) is a member of the Hepeviridae family capable of infecting humans producing a range of symptoms from mild disease to kidney failure. Epidemiological evidence suggests that hepatitis E genotype III and IV cases may be associated with the consumption of undercooked pork meat, offal and processed products such as sausages [1]. A study carried out by the Animal Health and Veterinary Laboratories Agency (AHVLA), found hepatitis E virus contamination in the UK pork production chain and that 10% of a small sample of retail pork sausages were contaminated with the virus [2]. Fu
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Author, Unknown. PR-138-907-R01 Test Kit Manual Field Sampling and Analysis of Contaminant Ions on Pipe Surfaces. Pipeline Research Council International, Inc. (PRCI), 1995. http://dx.doi.org/10.55274/r0012142.

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This document contains instructions for the sampling of steel pipe surfaces for contaminant ions using electrolytic limpet cell extraction or direct contact extraction with chromatographic papers, and analytical test methods for chloride, sulfate, nitrate/nitrite, and ferrous iron ions. The electrolytic limpet cell sampling method is the preferred technique for the recovery of ion contaminants from all pipe surfaces. Contact paper sampling may be used when operating conditions make the use of electrolytic limpet cell sampling impractical or inconvenient. Examples of sampling and analysis data
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