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Dissertations / Theses on the topic 'Metody identifikace'
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Skřivanová, Veronika. "Metody identifikace PHA produkujících bakterií." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240529.
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This diploma thesis deals with testing, optimazing and comparing methods for the identification of bacteria producing polyhydroxyalkanoates. Work included cultivation and microscopy methods, wherein the bacterial cells were stained with lipophilic dyes Nile red and Sudan black. Further, we also used flow cytometry and spectroscopic methods - Raman spectroscopy and infrared spectroscopy with Fourier transformation, and molecular biological methods, which analyzed the presence of a gene encoding PHA synthase (phaC) by polymerase chain reaction (PCR). PCR assay consist of two reactions, the firt on eis based on amplification of phaC gene along with 16S rRNDA gene, which is common for all the bacteria (multiplex PCR). The second reaction is focused on specific amplification of PHA synthase catalyzing biosynthesis of mcl-PHA. In order to overcome false positive results typical for methods analyzing genotype and also to avoid false negative results occuring in fenotype analyzing methods, the best strategy is to combine both aproaches. According to our results, analysis of presence of phaC gene by PCR can be combined with methods capable of determining presence of PHA in bacterial cells. For this purpose, Raman microspectroscopy seems to be very promising tool, since it is able to detect low content of PHA in cells and PHA can not be confused with other lipid metabolites. The results provide an overview of test methods, their advantages and disadvantages and also to compare different criteria according to which it is possible to choose the method of identification in depending on the adjustable requirements.
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Povalač, Aleš. "Metody a systémy prostorové identifikace RFID etiket." Doctoral thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2013. http://www.nusl.cz/ntk/nusl-233612.
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Disertační práce je zaměřena na metody a systémy pro měření vzdálenosti a lokalizaci RFID tagů pracujících v pásmu UHF. Úvod je věnován popisu současného stavu vědeckého poznání v oblasti RFID prostorové identifikace a stručnému shrnutí problematiky modelování a návrhu prototypů těchto systémů. Po specifikaci cílů disertace pokračuje práce popisem teorie modelování degenerovaného kanálu pro RFID komunikaci. Detailně jsou rozebrány metody měření vzdálenosti a odhadu směru příchodu signálu založené na zpracování fázové informace. Pro účely lokalizace je navrženo několik scénářů rozmístění antén. Modely degenerovaného kanálu jsou simulovány v systému MATLAB. Významná část této práce je věnována konceptu softwarově definovaného rádia (SDR) a specifikům jeho adaptace na UHF RFID, která využití běžných SDR systémů značně omezují. Diskutována je zejména problematika průniku nosné vysílače do přijímací cesty a požadavky na signál lokálního oscilátoru používaný pro směšování. Prezentovány jsou tři vyvinuté prototypy: experimentální dotazovač EXIN-1, měřicí systém založený na platformě Ettus USRP a anténní přepínací matice pro emulaci SIMO systému. Závěrečná část je zaměřena na testování a zhodnocení popisovaných lokalizačních technik, založených na měření komplexní přenosové funkce RFID kanálu. Popisuje úzkopásmové/širokopásmové měření vzdálenosti a metody odhadu směru signálu. Oba navržené scénáře rozmístění antén jsou v závěru ověřeny lokalizačním měřením v reálných podmínkách.
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Minařík, Martin. "Strukturální metody identifikace objektů pro řízení průmyslového robotu." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2009. http://www.nusl.cz/ntk/nusl-233840.
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This PhD thesis deals with the use of structural methods of objects identification for industrial robots operation. First, the present state of knowledge in the field is described, i.e. the whole process of objects recognition with the aid of common methods of the syntactic analysis. The main disadvantage of these methods is that is impossible to recognize objects whose digitalized image is corrupted in some ways (due to excessive noise or image disturbances), objects are therefore deformed. Further, other methods for the recognition of deformed objects are described. These methods use structural description of objects for object recognition, i.e. methods which determine the distance between attribute descriptions of images. The core part of this PhD thesis begins in Chapter 5, where deformation grammars, capable of description of all possible object deformations, are described. The only complication in the analysis is the ambiguity of the deformation grammar, which lowers the effectiveness of the analysis. Further, PhD thesis deals with the selection and modification of a proper parser, which is able to analyze a deformation grammar effectively. Three parsers are described: the modified Earley parser, the modified Tomita parser and the modified hybrid LRE(k) parser. As for the modified Earley’s parser, ways of its effective implementation are described. One of the necessary parts of the object recognition is providing the invariances, which this PhD thesis covers in detail, too. Finally, the results of described algorithms are mentioned (successfulness and speed of deformed objects recognition) and suggested testing environment and implemented algorithms are described. In conclusion, all determined possibilities of deformation grammars and their results are summarized.
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Veselý, Ivo. "Identifikace parametrů synchronního motoru." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2009. http://www.nusl.cz/ntk/nusl-217757.
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We often use a vector control for controlling of synchronous motor with permanent magnets. To get this control more effective we need to know the parameters of controlled motor. This document describes several methods, which deal with this problem and they are trying to expand them. We are talking about method uses Ohm’s law and the knowledge of motor model and method uses improved frequency analysis. The text below includes the principles of these methods and also identification verification of parameters by means of simulations on a created motor model. The final result of this work is the library of functions, which identificate on the basis on the improved frequency analysis.
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Hradilová, Lenka. "Robustní regrese - identifikace odlehlých pozorování." Master's thesis, Vysoká škola ekonomická v Praze, 2017. http://www.nusl.cz/ntk/nusl-359261.
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This master thesis is focused on methods of outlier detection. The aim of this work is to assess the suitability of using robust methods on real data of EKO-KOM, a.s. The first part of the thesis provides an overview and a theoretical treatise on classic and robust methods of outlier detection. These methods are subsequently applied to the obtained data file of EKO-KOM, a.s. in the practical part of the thesis. At the conclusion of the thesis, there are recommendations about suitability of methods, which are based on comparison of classical and robust methods.
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Šťávová, Karolína. "Identifikace abnormálních EKG segmentů pomocí metody Multiple-Instance Learning." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442555.
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Heart arrhythmias are a very common heart disease whose incidence is rising. This thesis is focused on the detection of premature ventricular contractions from 12-lead ECG records by means of deep learning. The location of these arrhythmias (key instances) in the record was found using a technique based on Multiple-Instance Learning. In the theoretical part of the thesis, basic electrophysiology of the heart and deep learning with a focus on the convolutional neural networks are described. Afterward, a program was created using the Python programming language, which contains a model based on the InceptionTime architecture, using which classification of the signals into the selected classes was performed. Grad-CAM was implemented to find locations of the key instances in the ECGs. The evaluation of the arrhythmia detection quality was done using the F1 score and the results were discussed at the end of the thesis.
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Kupková, Kristýna. "Metody rychlého srovnání a identifikace sekvencí v metagenomických datech." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-242103.
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Předmětem této práce je vytvoření metody sloužící k identifikaci organismů z metagenomických dat. Doposud k tomuto účelu spolehlivě dostačovaly metody založené na zarovnání sekvencí s referenční databází. Množství dat ovšem s rozvojem sekvenačních technik rapidně roste a tyto metody se tak stávají díky své výpočetní náročnosti nevhodnými. V této diplomové práci je popsán postup nové techniky, která umožňuje klasifikaci metagenomických dat bez nutnosti zarovnání. Metoda spočívá v převedení sekvenovaných úseků na genomické signály ve formě fázových reprezentací, ze kterých jsou následně extrahovány vektory příznaků. Těmito příznaky jsou tři Hjorthovy deskriptory. Ty jsou dále vystaveny metodě maximalizace věrohodnosti směsi Gaussovských rozložení, která umožňuje spolehlivé roztřídění fragmentů podle jejich příslušnosti k organismu.
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Honza, Marek. "Postupy a metody identifikace podnikových rizik a jejich příčin." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2017. http://www.nusl.cz/ntk/nusl-367519.
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The diploma thesis is focused on a selection of company, which an identification of risks and their causes is elaborated for. The selected company is AB JET spol. s.r.o., where systematic identification of company's risks and their core causes, evaluation of discovered risks' weight and specification of possible negative consequences were performed by using method IPR (Identification of Processes and Risks). Processed results should be used as a template for suggestions of preventive measures.
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Veselý, Ivo. "Identifikace parametrů synchronního motoru s permanentními magnety." Doctoral thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-263353.
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The purpose of this dissertation is to design identification methods for identifying a permanent magnet synchronous motor. The whole identification and motor control is carried out in d-q coordinates, and the program used for processing and control was the matlab simulink, together with the real time platform DSpace. The work focuses on two main areas of identification, off-line identification and on-line identification. For offline identification the frequency analysis was used with the lock rotor test to get three main parameters. They are the quadrature and direct inductances and stator resistance. In the online mode, the identified parameters were extended to magnet flux _f identified by MRAS method. The remaining parameters were again identified by frequency analysis, which was adapted into online mode, and simultaneously applied to the identification of several part in one time. The next method is Newton method, which is used for estimating stator resistance of the motor, without the need to apply any signal.
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Krzyžanek, Jakub. "Identifikace osob pomocí bipedální lokomoce." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2010. http://www.nusl.cz/ntk/nusl-218361.
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The aim of this thesis is to recognize a walking person in a sequence of images by defining his or her reference points to compare the course of their movement and then to identify the scanned person. Methods „k-means“ and „mean shift“ are used to obtain the silhouette of the person. However “environment model estimation” method is used here before those mentioned above. It is a type of a difference method and it helps to specify the scanning area and shortens the time of segmentation. During the search for the reference points the thesis focuses on three areas: the centre of the head and both ankle joints. Those points are later determined on the previous image sequence and compared with the real locations of the centre of the head and ankle joints marked by the user. The thesis also focuses on comparing the movement courses of those points and tries to identify the people whose walks are being scanned. Problematic situations which occurred during the whole process are analyzed in the end. The result of the thesis is an algorithm which can locate a moving person in an image sequence (video) and determine the reference points (centre of the head and ankles) to compare them and identify the scanned person.
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Jurečková, Nela. "PCR identifikace nepatogenních bakterií izolovaných ze sýrů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-216587.
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Different species of genus Bifidobacterium are part of human and animal intestinal flora. These bacteria have benefit effects and therefore they are used in foods and pharmaceutical products as probiotics. Cheese is now suitable as a probiotic matrix except yoghurts and fermentated milks. This diploma thesis was focused on optimalization of DNA isolation from bacteria of genus Bifidobacterium. Magnetic microparticles (P(HEMA-co¬-GMA)) were used for DNA isolation in presence of 8% polyethyleneglycol PEG 6000 and 5 M sodium chloride. Phenol extraction weas also used as an isolation method. Isolated DNA was used for amplification in domain, genus and species specific PCRs. Optimized method was tested for detection of bacteria of genus Bifidobacterium in experimentaly prepared probiotic cheeses. These cheeses contained potential probiotic bacteria from Laktoflóra collection. Bacteria were identified into species using species specific PCR. Species Bifidobacterium animalis was identified in all samples of probiotic cheeses.
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Drábková, Pavlína. "Identifikace faktorů výkonnosti podniku působícího v oboru ICT." Master's thesis, Vysoké učení technické v Brně. Fakulta podnikatelská, 2012. http://www.nusl.cz/ntk/nusl-223581.
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The diploma thesis focuses on finding and identification of performance factors of the company by means of intercompany comparisons methods. It analyzes the company which operates in the field of information and communication technologies and is compared with companies in related field of national economy. The performance factors of the company are selected by means of correlation analysis, which influences the performance of the company, afterwards there are compared the positions selected of companies by means of intercompany comparison methods in particular years.
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Jakubíček, Roman. "Metody segmentace a identifikace deformovaných obratlů ve 3D CT datech onkologických pacientů." Doctoral thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2020. http://www.nusl.cz/ntk/nusl-433053.
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In this doctoral thesis, the design of algorithms enabling the implementation of a fully automatic system for vertebrae segmentation in 3D computed tomography (CT) image data of possibly incomplete spines, in patients with bone metastases and vertebral compressions is presented. The proposed algorithm consists of several fundamental problems: spine detection and its axis determination, individual vertebra localization and identification (labeling), and finally, precise segmentation of vertebrae. The detection of the spine, specifically identifying its ends, and determining the course of the spinal canal, combines several advanced methods, including deep learning-based approaches. A novel growing circle method has been designed for tracing the spinal cord canal. Further, the innovative spatially variant filtering of brightness profiles along the spine axis leading to intervertebral disc localization has been proposed and implemented. The discs thus obtained are subsequently identified via comparing the tested vertebrae and model of vertebrae provided by a machine-learning process and optimized by dynamic programming. The final vertebrae segmentation is provided by the deformation of the complete-spine intensity model, utilizing a proposed multilevel registration technique. The complete proposed algorithm has been validated on testing databases, including also publicly available datasets. This way, it has been proven that the newly proposed algorithms provide results at least comparable to other author’s algorithms, and in some cases, even better. The main strengths of the algorithms lie in high reliability of the results and in the robustness to even strongly distorted vertebrae of oncological patients and to the occurrence of artifacts in data; moreover, they are capable of identifying the vertebra labels even in incomplete spinal CT scans. The strength is also in the complete automation of the processing and in its relatively low computational complexity enabling implementation on standard PC hardware. The system for fully automatic localization and labeling of distorted vertebrae in possibly incomplete spinal CT data is presented in this doctoral thesis. The design of algorithms enabling the implementation utilizes several novel approaches, which were presented at international conferences and published in the journal Jakubicek et al. (2020). Based on the results of the experimental validation, the proposed algorithms seem to be routinely usable and capable of providing fully acceptable input data (identified and precisely segmented vertebrae) as needed in the subsequent automatic spine bone lesion analysis.
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Strnadová, Michala. "Identifikace faktorů ovlivňujících hodnotu strojírenských podniků v ČR." Doctoral thesis, Vysoké učení technické v Brně. Fakulta podnikatelská, 2016. http://www.nusl.cz/ntk/nusl-254195.
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This doctoral thesis is focused on value based management. The basic principle of value based management states that the main object in an organization is value enhancement. Within an organization the concept of value based management connects activities and people participating in business processes to maintain that the resources the owners put into the business are evaluated. The essential task of value based management is to find and identify factors which contribute increasing performance and creating the value of a company, known as value drivers. The purpose of this thesis is to identify the factors which by significant amount affect the value of the mechanical engineering enterprises in the Czech Republic. In the first part of the thesis an analysis of current academic knowledge in area of value drivers is made. The base of the analysis is created by foreign resources because there is only limited amount of information in domestic literature. Besides the determination of value approach and its benefits attention is mainly paid to organizing results from value generators area. Findings of secondary resources analysis are used for own research problem. The second part of the thesis includes determination of the main objects and the research problem as well as formulation of the research questions and tested hypotheses. The third part is focused on describing methods which were used in the thesis. In the thesis was applied logical (induction – deduction, analysis – synthesis, generalization – concretaization) and empirical methods (comparsion method, quantitative research methods). Primary data was collected by using a questionnaire. Obtained data was analyzed using logarithmic decomposition of the return on equity, Boosted Trees and descriptive statistics (for example measures of location, measures of variability) and more statistical methods for analysis of statistical hypothesis (Kolmogorov-Smirnov test, Lilliefors test for normality, F-test, Student’s t-test, Chi-squared test, Fisher’s exact test, Spearman’s rank correlation coefficient). In the fourth part of the thesis the branch of manufacturing industry is characterized and the identification of performance factors is accomplished applying logarithmic decomposition of the return on equity. The other subjects of research were companies in a branch called “Manufacturing of machines and devices”. The fifth part of the thesis includes summary of findings of the prime research and its discussion. Primary data was obtained by a questionnaire survey and completed with accounting information of respondents. The findings of the research show that main determinants affecting the value of engineering companies in the Czech Republic are return on assets, profit margin, consumption, sales profitability, personnel costs and value added. The qualitative value drivers are good reputation, human resources (employees) and its characteristics, business and flexibility, performance and productivity, assets, competency responsible staff, attentiveness to requirement of customers, innovation and quality.
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Zemanová, Lucie. "Identifikace únavového poškození Al slitiny zpracované technologií SLM s využitím metody akustické emise." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2016. http://www.nusl.cz/ntk/nusl-241701.
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The aim of the diploma thesis is to study fatigue properties of EN AW-2618A aluminium alloy produced by selective laser melting (SLM). S-N diagrams of reference alloy and SLM material depending on manufacturing conditions were constructed. Reference alloy had the same chemical composition as the SLM material, but it was made by traditional technology (by extruding). While testing, acoustic emission (AE) and resonant frequency of loading was monitored. Fractographic analyses of fracture surfaces were performed. It was found, that SLM material has significantly worse fatigue performance. Fatigue properties are strongly dependent on SLM process parameters settings and precise observance of procedure. The most significant differences in cyclic degradation were found in the length of the stages of fatigue. The more homogeneous the material is, the longer the nucleation stage compared to the total lifetime is. The main source of AE in case of the reference material is plastic deformation on the tip of the crack. For SLM testing, stronger acoustic activity was recorded, because brittle fracture of the material between discontinuities, which is stronger AE source, is more frequent.
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Olivová, Radana. "Optimalizace metody PCR-RFLP pro taxonomické zařazení kvasinek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216560.
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This thesis deal with optimalization method PCR – RFLP for taxonomy enlistment of yeasts. Conventional identification methods for yeasts are time-consuming. Molecular biological method based on PCR are instrumental towards fast and precise identification as compared to conventional phenotypic methods. In this thesis molecular biological method PCR – RFLP was used for identification and enlistment of yeasts. This metod follow repeating spacers of ribozomal DNA of yeast, characteristic for each species and strain. By the help of PCR were amplified specific partitions of DNA. These fragments of DNA were split by restriction endonucleases and identified by horizontal electroforesis. In background of this thesis there are information about yeasts, their taxonomy and molecular biological methods.
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Jeník, Ivan. "Identifikace parametrů elasto-plastických modelů materiálu z experimentálních dat." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2015. http://www.nusl.cz/ntk/nusl-231979.
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This master's thesis deals with the identification of the material flow curve from record of tensile test of smooth cylindrical specimen. First, necessary theory background is presented. Basic terms of incremental theory of plasticity, tensile test procedure and processing its outputs are described. Furthermore, possibilities of mathematical expression of the elastic-plastic material constitutive law, thus mathematical expression of the material flow curve itself. Mechanism of ductile damage of material is explained briefly as well. Overview of recent methods of the flow curve identification is given, focused on cases, when the stress distribution in a specimen is not uniaxial. That is either kind of analytic correction of basic formulas derived for uniaxial stress state, or application of mathematical optimization techniques combined with numerical simulation of the tensile test. Also unusual method of neural network is mentioned. For 8 given materials, the flow curve identification was performed using different methods. Namely by analytic correction, optimization, sequential identification and neural network. Algorithms of the last two methods were modified. Based on assessment of obtained results, application field and adjusting the parameters of single algorithms was recommended. It showed up, that an effective way to the accurate and credible results is the combination of different methods during flow curve identification procedure.
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Zátopek, Michal. "Metody strojového vidění pro rozpoznání dopravního značení." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-221305.
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This diploma thesis is dealing with an issue of machine vision in the field of traffic signs recognition. The first part is devoted to machine vision in general traffic situations. Together with traffic applications there is paid attention to a possible autonomous vehicle and applications for the traffic signs recognition. The main part of this work is devoted to a description and an implementation of several methods for colour and pattern localisation of traffic signs in the scene and to identification algorithms. Apart from the implementation itself, these algorithms are submitted to several experiments for a valorisation of their success. The thesis also includes a gallery of images with traffic signs including a file with descriptive annotation for an automatic testing of algorithms.
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Houfek, Martin. "Návrh a vývoj experimentálního zařízení pro testování kloubní jamky TEP kyčelního kloubu a identifikace otěru pomocí optické metody." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2011. http://www.nusl.cz/ntk/nusl-233946.
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Today is the implantation of joint replacements, although a radical, but very functional restoration frequent way ill joint. The implants are placed high demands on their biocompatible and long-term function, which is directly related to wear of joint replacements in the human body. Therefore, analysis of the complex behavior of the implant in the human body is a fundamental problem of biomechanics of implants. This issue is dedicated, this dissertation thesis. Dissertation thesis deals with the solution design and development of experimental device for testing the TEP acetabula and wear identification using optical method. The proposed device allows the joint burden hole TEP physiological manner, but by working with the acetabulum burdened by Power resultant joint axis passes through the hole. The physiological loading of articular wells and evaluating the size of the loss of polyethylene is currently intensively addressed. The results of this study may in the near future, serving as information for assessing the conduct of wear prosthetic hip joint in vivo. The work is devoted to solving the problem of the size required to detect the loss of polyethylene for joint replacement arthroplasty: Design and development of experimental equipment for testing the acetabulum of the hip joint arthroplasty. Identify wear using optical methods. Design and development of experimental equipment was made in the system Inventor Professional 2010 at the level of 3D modeling. Great attention was paid to the design fit into the acetabulum TEP testing machines and gripping the acetabulum into an optical device. Management options were tested with combinations of experimental equipment engines. Suggestions were made and then tests for fixing the acetabulum into an optical device. Has the possibility of depth analysis and identification of wear joined with innovative solutions to evaluate the loss of polyethylene acetabulum arthroplasty. Identification of abrasion (loss of polyethylene) joint arthroplasty holes were carried out experimental measurements using an optical holographic interferometry. The comprehensive analysis of the results of experimental measurement of the loss of polyethylene shows that this method can identify both the size but also an area where there is even to small changes in the surface, which depend on the abrasion. One can also say that using this method we are able to affect the will or displacements that affect the size of the loss of polyethylene in joint arthroplasty hole. Despite the number of known and lessons learned arising from the results of solving the problem of design and development of experimental equipment for testing the acetabulum and hip arthroplasty wear identification using optical methods, this work is that the initial study to identify problematics wear of joint replacements. Complete resolution of this issue is beyond the scope of one doctoral thesis.
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Šuranská, Hana. "Izolace, identifikace a charakterizace mikroflóry vína a vybraných potravin." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-233385.
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Proposed dissertation thesis deals with wine and artisanal cheeses microbiology. The first part is focused on identification of yeasts isolated from grapes and musts during production of white and red wines. The grape varieties were grown under the integrated and organic farming on Moravian vineyard. Yeasts were identified by ITS-PCR-RFLP method (amplifying internal transcribed spacer ITS: ITS1, ITS2 and 5.8S rDNA) and unknown species were subjected to partial sequencing of ITS rDNA region. In total, 524 isolates were divided into 14 different species belonging to six genus were identified from. The first stages of fermentation process were characterised by predominance of non-saccharomyces species especially H. uvarum. Due to increased ethanol concentration strains of S. cerevisiae prevailed in the later phases of the process. Further, partial aim of this study was to isolate and to apply selected autochthonous S. cerevisiae strains as starter culture during controlled industrial wine fermentation process. Genus Saccharomyces was distinguished from other non-saccharomyces species by ITS-PCR-RFLP. Further, in order to distinguish Saccharomyces genus at the species and the strain level, several molecular methods were applied including PCR-fingerprinting (rep- and RAPD-PCR), species-specific primers (multiplex and touchdown PCR), LSU-DGGE and interdelta PCR. Species-specific primers enabled us to distinguish some species of the Saccharomyces sensu stricto complex. Furthermore, interdelta PCR seems to be useful tool for S. cerevisiae strains identification. Among 120 isolated autochthonous strains belonging to Saccharomyces genus, 45 different strains were identified. Based on its sufficient technological properties (osmo- and ethanol tolerance, low H2S production etc.), S. cerevisiae 1-09 strain isolated from grape berries coming from moravian vineyard was chosen. Strain S. cerevisiae 1-09 was tested in small amount of must and after that also during industrial fermentation of red and white wine production. Based on the results of chemical and sensorial analysis, the strain seems to be suitable for application as the starter culture for winemaking process. The final part of this thesis is focused on quantification and identification of the yeasts isolated from artisanal cheeses and their by-products coming from Western Balkan Countries. Isolated species were identified by ITS-PCR-RFLP, partial sequencing and by physiological tests. Among the 20 yeast species found, D. hansenii, C. zeylanoides and Y. lipolytica were found to be predominant. Moreover, we developed culture-independent, semi-quantitative technique based on construction of ITS-clone library from metagenomic DNA to investigate complex fungal communities associated with artisanal cheeses and their by-products. Novel technique is based on direct extraction of total DNA from the sample. This was compared with culture-dependent ITS-PCR-RFLP and culture-independent LSU-DGGE methods. The results highlighted the discrepancies among these methods. Finally, the divergences among applied methods were confirmed by correlation analysis and by indices of general biodiversity and dominance of species. ITS-clone library approach combines the advantages of cultivation-based analysis and LSU-DGGE with semi-quantification of fungal species without the requirement of their cultivation. This study might open new perspectives in direct and complex analysis of yeasts and moulds in food matrices.
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Tomaštík, Filip. "Vybrané postupy a metody vedoucí k rozpoznání rizik vybraného subjektu podnikajícího v oblasti služeb." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2019. http://www.nusl.cz/ntk/nusl-402660.
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The thesis deals with strategic and financial analysis in the company Účetnictví a daně OSNOVA, s.r.o. It is divided into four parts. In the first part, the thesis deals with the theoretical basis according to the literature. The analytical part introduces the company. In the practical part, strategic and financial analyzes are performed. In the last part, conclusion and suggestions, concrete measures to reduce the value of risks resulting from the performed analyzes are then discussed.
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Cipín, Radoslav. "Moderní matematické metody syntézy a analýzy střídavých strojů." Doctoral thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-233566.
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The doctoral thesis is divided into three main parts with a focus on induction motors. The first part deals with the electromagnetic design and analysis of windings of AC ma-chines. There is introduced the density of conductors, which describes the distribution of the windings in the slots. There is derived general formula for calculation of the total winding factor applicable to any type of winding. The second part of this work deals with the acceleration method of the induction motors torque-speed characteristics measurement. The measured characteristics are used for exact identification of the equivalent circuit parameters of induction motor. The third section is focused on a very precise identification of the equivalent circuit parameters of induction motor in the form of -circuit. The identification is based on mutual comparison of measured and calculated torque-speed and current-speed characteristics.
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Janošík, Petr. "Rozhodovací metody v managementu rizik." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2011. http://www.nusl.cz/ntk/nusl-236999.
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This thesis deals with the matter of risk managament in IT projects. It explains the importance of risk management in such projects and shows different ways and methods of managing and analyzing the risks. After explaining the basic concepts and the various phases of risk management the text focuses on two methods of risk analysis - the fault tree analysis of event tree analysis. Use of both methods is explained for both quantitative and qualitative analyses. The second half of the work includes the design of an application for the support of risk analysis employing the methods of fault tree analysis and event tree analysis. This is followed by a description of the implementation of the proposed system in a web environment using jQuery, Nette Framework and Dibi.
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Černá, Jana. "Metody a nástroje znalostního managementu." Master's thesis, Vysoká škola ekonomická v Praze, 2012. http://www.nusl.cz/ntk/nusl-194191.
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The Master's Thesis is focused on methods and tools in knowledge management. The paper introduce knowledge management its history and present. Meanwhile history is presented as a list of important events present view is supported by current trends in field of knowledge management, business analysis of selected companies and evaluation of available publications on the topic of knowledge management. The main aim the paper is to present suitable methodology for the initial phase of Mitroff's model - conceptualization. The selected approach is then applied to the model situation. It is the decision of the city government for the construction and expansion of local factory. The last part is about Business Model Canvas which aims to outline the possible application of the methodology in practice.
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Zimek, Tomáš. "Software pro identifikaci dynamických systémů." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2019. http://www.nusl.cz/ntk/nusl-399369.
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The thesis deals with methods of identification of dynamic systems in time and frequency domain. Nonparametric and parametric methods of identification are analyzed. Selected methods are implemented in Matlab & Simulink sotware. Finally, the user guide for the created Python application is introduced.
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Křivánková, Petra. "Řízení rizik společnosti Energopoint, spol. s r.o." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2015. http://www.nusl.cz/ntk/nusl-234433.
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The thesis deals with risk management in the company Energopoint Ltd. The theoretical part explains important concepts related to the issue. This primarily involves the identification, analysis and follow-up methods of risk reduction. In the analytical part are identified all possible risks a company that has been detected by analysis of macro environment, micro environment and internal analysis of the company. To evaluate the significance of risks is used scoring method. Based on the findings of the analytical part I proposed measures to reduce risks. The contribution of the work is to gain the advantages of introducing risk management in the company.
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Grygarová, Lucie. "Řízení rizik podnikatelského subjektu." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2016. http://www.nusl.cz/ntk/nusl-241253.
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The thesis deals with risk management of business entity, which provides services in field of education officials. Risk management proces consists of several parts, such as identification, analysis and risks reduction. All of these parts, including basic concepts, are described in the theoretical part. In the analytical part there are methods, which are using for identification potential risks. It include SLEPT analysis, Porter analysis and 7S model, then comes SWOT analysis. Based on these analyzes are proposed measures and recommendations that will help reduce risks.
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Zámečník, Josef. "Řízení rizik podnikatelského subjektu." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2017. http://www.nusl.cz/ntk/nusl-318121.
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The thesis deals with the risk management in Motortec s.r.o., a company that operates in the field of sales and maintenance service of passenger and commercial vehicles. The thesis is divided into the theoretical part, in which all the essential terms are defined that relate to the thesis itself, and an analytical part that employs the process of risk management. The process includes identification, analysis and subsequent remedies to risks identified. The identification of risks has been carried out using Porter's five forces analysis, SLEPT analysis and McKinsey 7S Framework. SWOT analysis has been carried out subsequently. The risks defined have been assessed using a scoring method and grouped into categories based on their severity. The last part includes measures to reduce the major risks that threaten the examined company.
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Lenková, Petra. "Vzdělávání a rozvoj pracovníků." Master's thesis, Vysoká škola ekonomická v Praze, 2011. http://www.nusl.cz/ntk/nusl-113294.
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The Master's Thesis deals with the problematic of education and development of employees. The thesis is divided into theoretical and practical part. The theoretical part introduces the background for the efficient functioning of the system of education and development of employees worked out on literature. Practical part compares the theory with reality. The aim of this thesis is to analyse the system of staff education and development in Henkel company and propose suggestions for improvement of current system.
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Herman, Tomáš. "Identifikace klopné tuhosti nápravy automobilu." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-378016.
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This diploma thesis deals with roll stiffness of twist beam axle. There are described experi-mental and analytic methods of measuring, its applications and comparison on a particular type of axle. There is also construction plan of system usable for this measuring.
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Macenauer, Oto. "Identifikace obličeje." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2010. http://www.nusl.cz/ntk/nusl-237221.
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This document introduces the reader to area of face recognition. Miscellaneous methods are mentioned and categorized to be able to understand the process of face recognition. Main focus of this document is on issues of current face recognition and possibilities do solve these inconveniences in order to be able to massively spread face recognition. The second part of this work is focused on implementation of selected methods, which are Linear Discriminant Analysis and Principal Component Analysis. Those methods are compared to each other and results are given at the end of work.
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Dulesov, Egor. "Identifikace významných spektrálních složek ve stresovém řečovém signálu." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-241000.
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The aim of this master’s thesis is to learn the problem of analysis and identification of significant spectral components in speech signal. Based on learning a special literature chooses the suitable methods of spectrum estimate. Does learning the literature in specification of testing of spectral components significate. Makes a procedure for identification of chosen speech formants. Does this procedure for audio signals both of in stress and in normal state. Estimates the results, compares efficiency of chosen methods and determine threshold for chosen formant of analyzed stress signal. States the recommendations for speech spectral analysis in stress situation.
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Průša, David. "Identifikace tepelné vodivosti a tepelné kapacity stavebních látek metodou „Hot Wire Method“." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2019. http://www.nusl.cz/ntk/nusl-392340.
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This aim of task deals with study of heat dissipation mechanisms and the description of physical phenomena, which is accompanied by non-stationary measurement of thermal characteristics by the method "hot-wire method". In particular, we observe the coefficient of thermal conductivity and its dependence on various variables such as the temperature of the measured sample, its moisture state, the volume of the sample and its porosity. The above mentioned findings are used for the invention of the measuring device of a nonstationary gauge, which is based on regular heating and is dedicated to measuring the thermal conductivity coefficient and the heat capacity by the "hot-wire method" method. In the last part of the thesis is verified functionality of the proposed measuring device, the suitability of the created algorithm for the processing of the measured data and the evaluation of the results was verified. The reproducibility of the measurements was verified and the measured results were compared with the measurement methods, which are commonly used. the influence of humidity on the coefficient of thermal conductivity.
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Lebeda, Aleš. "Model soustavy motorů s pružným členem." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-219693.
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This thesis deals with problem of experimental identification using principles of artificial intelligence and development of nonlinear models. It shows how to estimate parameters of nonlinear models and it compares different types of nonlinear models based on analytical analysis which were developed from measured data in simulation and real system motors with flexible component.
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Dušek, Martin. "Identifikace parametrů synchronních motorů s permanentními magnety." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218942.
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This work deals with the on-line identification of permanent magnet synchronous motor parameters. There is discussed the use of four different identification algorithms based on the least squares method and MRAS. The functionality of the algorithms is verified in Matlab - Simulink environment. Simulation results are compared in terms of rate and accuracy of identification, resistance to noise and other factors.
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Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.
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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after repurifying by the ethanol and drying submitted to the restriction analysis. With use of the restriction endonuklases DNA is chopped into the specific segments typical for the particular genus. The chopped fragments can be separated in the electric field in the agarose gel and subsequently evaluated. In this thesis together 63 type yeasts were used. These yeasts were analysed by applying of the seven restriction endonuklases – HaeIII, HhaI, HinfI, HpaII, TaqI, AluI a MseI. The final image of type yeasts splitting was compared to the results of splitting of already identified wine yeasts and these yeasts were subsequently taxonomically classified. Evaluation of genetic similarity was conducted by program BioNumerics and as the results the dendrograms that were created with use of Jaccard‘s coefficients are obtained.
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Jenča, Pavol. "Identifikace parametrů elektrických motorů metodou podprostorů." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-219678.
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The electrical motors parameters identification is solved in this master’s thesis using subspace based methods. Electrical motors are simulated in Matlab/Simulink interactive environment, specifically permanent magnet DC motor and permanent magnet synchronous motor. Identification is developed in Matlab interactive environment. Different types of subspace algorithms are used for the estimation of parameters. Results of subspace parameters estimation are compared with least squares parameters estimation. The thesis describes subspace method, types of subspace algorithms, used electrical motors, nonlinear approach of identification and comparation of parameters identification.
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Macalík, Radek. "Identifikace regulované soustavy, jednotka pro spalování biomasy." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2008. http://www.nusl.cz/ntk/nusl-228075.
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This thesis dissertates the identification of existing experimental unit of biomass combustion. It analyses particular methods of identification, the selection of a model and methods of the estimation of parameters, especially by using last square method and analysis of time-response characteristic. It describes common identification procedure and particular algorithms which were used in practice for obtaining model’s parameters. The thesis also mentions popular software tools used for the modeling and the identification of systems. It comprises the implementation of obtained mathematic models using programming environment Borland Delphi as well.
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Tůma, Milan. "Identifikace a hodnocení preferencí zákazníků u vybraného produktu." Master's thesis, Vysoké učení technické v Brně. Fakulta podnikatelská, 2019. http://www.nusl.cz/ntk/nusl-401995.
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Finding customer preferences when deciding about buying a tractor in the 201-300 horsepower category. Attributes will be gained through questionnaire survey, and will be used in the QFD method, where there will also be comparison of major competitors in a given market segment. The evaluation of tractors will also include the voice of the customer, gained from KANO model. KANO model will be second survey that will be used in this thesis. The conclusion is to build an ideal tractor structure that matches the customer's wishes.
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Šubrt, Stanislav. "Návrh přístroje pro analýzu vzniku a šíření trhlin." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-231511.
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The main goal of this thesis is to get an insight into a field of non-destructive testing using potential drop techniques that have nowadays become the standard not only in the fatigue and loading tests but also in the industry. These methods can serve to non-destructively and continuously measure material specimens, thickness, corrosion losses, deformations, spectroscopy and detection and analysis of crack geometry. They can help to identify materials and measure material changes over time. The second part of this thesis deals with designing the aperture for detection of cracks in steam and product piping using potential drop technique modified by Ing. Ladislav Korec, CSc. Last part deals with extensive testing, experimenting and evaluation of the aperture.
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Dokoupil, Jakub. "Rozšířená kvadraticky optimální identifikace a filtrace." Doctoral thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-233562.
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Simultaneous evaluation of the whole set of the model parameters of different orders together with an ability to track unmodeled dynamics are desired features in the tasks of parameter estimation. A technique handling with the factors produced by an augmented covariance (ACM) or information (AIM) matrices is considered to be an appropriate tool for designing multiple model estimation. This is where the name augmented identification (AI) by using the least-squares method was taken. The method AI attains numerical stability of the calculation of the conventional least squares method while in the same time, fully extracts information contained in the observation. In order to track time varying parameters can be found that all the information pertinent to recursive identification and thus to data driven forgetting is concentrated in ACM as well as in AIM. In this thesis will be introduced how to selective forgetting to ACM should be applied in an effective way. It means forget only a portion of accumulated information which will be further modified by the newest data included in the regressor. In the estimation problems the knowledge of the inner states of the identified system is often required. Because the augmented identification belongs within the class so called prediction error method (PEM), some rational requirements can be deduced. As a result, state filter should constitute optimization procedure minimizing the predicted error of given state space model representation with respect to the vector of states. The proposed scheme will considerably extend the family of algorithms based on processing of ACM (AIM) about augmented filtering (AF). This all will establish a comprehensive concept of parametric estimation that compared with conventional approaches is characterized by versatility, low demands on a priori process information and by excellent numerical properties (robust against overparametrization, capable solving the multiple model problem).
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Hujňák, Jaroslav. "Identifikace 3D objektů pro robotické aplikace." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-416605.
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This thesis focuses on robotic 3D vision for application in Bin Picking. The new method based on Conformal Geometric Algebra (CGA) is proposed and tested for identification of spheres in Pointclouds created with 3D scanner. The speed, precision and scalability of this method is compared to traditional descriptors based method. It is proved that CGA maintains the same precision as the traditional method in much shorter time. The CGA based approach seems promising for the use in the future of robotic 3D vision for identification and localization of spheres.
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Kokeš, Miroslav. "Porovnání metod pro identifikaci poruch valivých ložisek." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442447.
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The aim of this master thesis is the comparison of selected methods and parameters for roller bearings diagnostics. Selected statistical parameters are kurtosis, crest factor, and parameter K(t). The other selected methods are envelope analysis, cepstral analysis, and ACEP method. These methods are implemented in LabVIEW software and compared based on noise resistance, computation speed, and overall capability of identifying roller bearing faults.
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Škvařil, Ondřej. "Jemné částice produkované krbovými kamny." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-417853.
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This master thesis deals with problematics of fine particles produced during combustion in fireplace stoves. The theoretical part is focused on the analysis of the atmosphere, health consequences, solid pollutants, the formation of particles (origin), particle separation and how they are regulated by laws. Theoretical section of the thesis addresses analysis of biomass and its combustion. In the applied section are listed given methods of the particles measuring according to regulations, common errors during measurement and methods of identification of measured fine particles. Then follow specific measurements of the formed very fine particles produced by the SMPS method. Subsequently, the results of the measurement are evaluated and summarized in the conclusion.
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Vaňková, Tereza. "Adaptivní regulátory s principy umělé inteligence a jejich porovnání s klasickými metodami identifikace." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218930.
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Master’s thesis is focused on the adaptive controllers. The first theoretic part mainly describes the parametric identification, which belongs to the most important part of the adaptive controller’s structure. Classical identification methods (the recursive least squares methods) are firstly mentioned and afterwards the identification methods based on the neural network (the Marquardt-Levenberg algorithm and the new identification algorithm NIA inspired by the neural networks) are described. At the conclusion of the theoretic part there are mentioned the algorithm of the adaptive controller’s tuning which uses the identification parameters (the modified Z-N method) and the tested types of adaptive controllers. Particular results, which were found out by verifying of the adaptive controllers on the simulation and real models, are contained in second, the practical, part of the thesis. Finally, achieved results are compared with the classical discrete PID controller and with the adaptive controller of the B&R company.
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Zikmund, Pavel. "Identifikace aerodynamických charakteristik atmosférického letadla z výsledků letových měření." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2013. http://www.nusl.cz/ntk/nusl-234162.
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The thesis deals with aerodynamic characteristics identification from flight measurement. The topic is part of flight mechanic – handling qualities. The first theoretic part consists of three identification methods description: Error equation method, Output error method and Filter error method. Mathematical model of an airplane is defined and restricted to the motion with 3 degree of freedom. There is also introduced simulation of flight measurement for identification software validation. Practical part is focused on experiment preparation, execution and evaluation. The airplane VUT 700 Specto had been chosen to carry out flight tests. The airplane was modified to the new electric powered VUT 700e Specto after first measurement flights with combustion engine. Data record from on-board measurement unit was completed by telemetric data from autopilot and remote control system. Flight tests were carried out in stabilised mode of autopilot in symmetric flight. The results were confronted with analytical analysis results and DATCOM+ software parameter estimation.
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Czabeová, Ivana. "Identifikace rizik prodejce automobilů pomocí vybraných metod ekonomické a strategické analýzy." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2017. http://www.nusl.cz/ntk/nusl-317083.
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The master´s thesis deals with the economic and strategic analysis in selected company. The aim is to identify risks that arise from the analyzes and suggest measures that will lead to the elimination of identified risks.
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Končír, Oliver. "Identifikace rizik podnikatelského subjektu pomocí vybraných metod ekonomické a strategické analýzy." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2017. http://www.nusl.cz/ntk/nusl-318549.
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This master´s thesis is focused on the analysis of risks, nowadays it is important that companies implement preventive identification and analysis of risk and eliminate these risks. In this thesis will be performed selected analyzes of the company, identyfying the risks that resulting form the analysis and design of measures to reduce certain risks.
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Kavková, Renáta. "Identifikace rizik webového studia pomocí vybraných metod ekonomické a strategické analýzy." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-382689.
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The subject of the diploma thesis is the analysis of Poski.com, s.r.o. Risk identification and proposal of measures. The work is divided into four parts. The first part focuses on the theoretical basis of work. The second part puts theory into practice using the given methods and analyzes. This followed by results assessment by risk analysis and RIPRAN method. The last part discusses appropriate measures.
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Režný, Tomáš. "Identifikace rizik podnikatelského subjektu pomocí vybraných metod ekonomické a strategické analýzy." Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-382712.
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This Master’s thesis deals with a strategic and economic analysis of a chosen company with objective to identify risks and formulate proposals and recommendations resulting in decreasing of risks or their potential elimination. The thesis is composed of three parts. First part contains theoretical framework needed for practical application which includes chosen methods of external and internal analysis with their evaluation and analysis of risks using RIPRAN method. Last part contains formulation of precautions based on the risks found.